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38 pages, 10567 KiB  
Article
A Bionic-Based Multi-Objective Optimization for a Compact HVAC System with Integrated Air Conditioning, Purification, and Humidification
by He Li, Bozhi Yang, Xinyu Gu, Wen Xu and Xuan Liu
Biomimetics 2025, 10(3), 159; https://doi.org/10.3390/biomimetics10030159 - 3 Mar 2025
Viewed by 296
Abstract
This study is dedicated to the development of a multifunctional device that integrates air conditioning, humidification, and air purification functions, aimed at meeting the demands for energy efficiency, space-saving, and comfortable indoor environments in modern residential and commercial settings. The research focuses on [...] Read more.
This study is dedicated to the development of a multifunctional device that integrates air conditioning, humidification, and air purification functions, aimed at meeting the demands for energy efficiency, space-saving, and comfortable indoor environments in modern residential and commercial settings. The research focuses on achieving a balance between performance, energy consumption, and noise levels by combining bionic design principles with advanced optimization algorithms to propose innovative design and optimization methods. Specific methods include the establishment and optimization of mathematical models for air conditioning, air purification, and humidification functions. The air conditioning module employs a nonlinear programming model optimized through the Parrot Optimizer (PO) Algorithm to achieve uniform temperature distribution and minimal energy consumption. The air purification function is based on a bionic model and optimized using the Deep ACO Algorithm to ensure high efficiency and low noise levels. The humidification function utilizes a mist diffusion model optimized through the Slime Mold Algorithm (SMA) to enhance performance. Ultimately, a multi-objective optimization model is constructed using the Beluga Whale Optimization (BWO), successfully integrating the three main functions and designing a compact segmented cylindrical device that achieves a balance of high efficiency and multifunctionality. The optimization results indicate that the device exhibits superior performance, with a Clean Air Delivery Rate (CADR) of 400 m3/h, a humidification rate of 1.2 kg/h, a temperature uniformity index of 0.08, and a total power consumption controlled within 1600 W. This study demonstrates the significant potential of bionic design and optimization technology in the development of multifunctional indoor environment control devices, enhancing not only the overall performance of the device but also the comfort and sustainability of the indoor environment. Future work will focus on system scalability, experimental validation, and further optimization of bionic characteristics to expand the device’s applicability and enhance its environmental adaptability. Full article
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<p>Flowchart of the PO algorithm.</p>
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<p>Flowchart of the DeepACO algorithm.</p>
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<p>Flowchart of the Slime Mold Algorithm.</p>
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<p>Flowchart of the BWO algorithm.</p>
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<p>Convergence of the PO algorithm.</p>
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<p>Impact of outlet parameters on air conditioning efficiency.</p>
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<p>Indoor temperature dynamics: summer vs. winter simulation.</p>
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<p>Impact of air conditioning dimensions on temperature distribution.</p>
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<p>Sensitivity analysis for air conditioning partial modeling.</p>
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<p>DeepACO model iteration process.</p>
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<p>Air purifier design drawing.</p>
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<p>Sensitivity analysis for air purifier partial modeling.</p>
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<p>SMA iteration process.</p>
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<p>Air humidifier visualization.</p>
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<p>Room humidity distribution and dynamics.</p>
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<p>Sensitivity analysis for humidifier partial modeling.</p>
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<p>BWO algorithm iteration process.</p>
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<p>The design of an all-in-one device.</p>
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<p>The effect visualization of the all-in-one device.</p>
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<p>Sensitivity analysis for tri-unit air conditioner modeling.</p>
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37 pages, 2739 KiB  
Review
Biologically Active Compounds in True Slime Molds and Their Prospects for Sustainable Pest and Pathogen Control
by Tomasz Pawłowicz, Konrad Wilamowski, Monika Puchlik, Igor Żebrowski, Gabriel Michał Micewicz, Karolina Anna Gabrysiak, Piotr Borowik, Tadeusz Malewski, Ewa Zapora, Marek Wołkowycki and Tomasz Oszako
Int. J. Mol. Sci. 2025, 26(5), 1951; https://doi.org/10.3390/ijms26051951 - 24 Feb 2025
Viewed by 196
Abstract
True slime molds (Eumycetozoa) represent a monophyletic clade within the phylum Amoebozoa, comprising the lineages Myxogastria, Dictyostelia, and Protosporangiida. Although historically misclassified as fungi, recent molecular and biochemical studies underscore their distinct evolutionary trajectories and rich metabolomic [...] Read more.
True slime molds (Eumycetozoa) represent a monophyletic clade within the phylum Amoebozoa, comprising the lineages Myxogastria, Dictyostelia, and Protosporangiida. Although historically misclassified as fungi, recent molecular and biochemical studies underscore their distinct evolutionary trajectories and rich metabolomic profiles. In this review, we synthesize current knowledge on Eumycetozoa as a reservoir of bioactive compounds, detailing how secondary metabolites—including polysaccharides, amino acids, unsaturated fatty acids, terpenoids, and glycosides—vary across plasmodia, fruiting bodies, and spores. A systematic literature search in major scientific databases accounted for legacy nomenclature and leveraged chemoinformatic tools for compound verification. Our findings reveal 298 distinct metabolites that serve ecological roles in nutrient recycling and interspecies interactions, while also showing promise for controlling agricultural pests and pathogens. Notably, certain glycosides, lectins, and polyketides exhibit antimicrobial or cytotoxic activities, indicating their potential utility in managing these biological challenges. By consolidating current data and emphasizing the wide taxonomic range of Eumycetozoa, this review highlights the critical need for comprehensive biochemical and genomic investigations. Such efforts will not only advance our understanding of slime mold metabolomes and their evolutionary significance but also pave the way for innovative, eco-friendly applications. Full article
(This article belongs to the Section Bioactives and Nutraceuticals)
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<p>Simplified classification of <span class="html-italic">Amoebozoa</span> highlighting the placement of <span class="html-italic">Eumycetozoa</span> and its main lineages (<span class="html-italic">Dictyostelia</span>, <span class="html-italic">Myxogastria</span>, and <span class="html-italic">Protosporangiida</span>). Original artwork by Tomasz Pawłowicz, based on the phylogenomic analyses of Tekle et al. (2022) [<a href="#B1-ijms-26-01951" class="html-bibr">1</a>], who used 824 single-copy genes (113,910 sites) from 113 taxa, analyzed with the maximum-likelihood (ML) software IQ-TREE (LG + G4 + C60 + F; 1000 ultrafast bootstrap replicates) and RAxML (Randomized Axelerated Maximum Likelihood; PROTGAMMALG4X).</p>
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<p>Diverse forms of plasmodia and fruiting bodies observed in various <span class="html-italic">Eumycetozoa</span>. (<b>A</b>) <span class="html-italic">Lycogala epidendrum</span>; (<b>B</b>) <span class="html-italic">Fuligo septica</span>; (<b>C</b>) <span class="html-italic">Comatricha nigra</span>; (<b>D</b>) <span class="html-italic">Arcyria cinerea</span>. Photos by Tomasz Pawłowicz.</p>
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<p>Life cycle of a plasmodial slime mold (<span class="html-italic">Myxogastria</span>) based on observations of <span class="html-italic">Comatricha nigra</span>. Original work by Igor Żebrowski and Tomasz Pawłowicz, based on Clark et al. [<a href="#B23-ijms-26-01951" class="html-bibr">23</a>] and Stephenson et al. [<a href="#B10-ijms-26-01951" class="html-bibr">10</a>].</p>
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20 pages, 4706 KiB  
Article
A SMA-SVM-Based Prediction Model for the Tailings Discharge Volume After Tailings Dam Failure
by Gaolin Liu, Bing Zhao, Xiangyun Kong, Yingming Xin, Mingqiang Wang and Yonggang Zhang
Water 2025, 17(4), 604; https://doi.org/10.3390/w17040604 - 19 Feb 2025
Viewed by 301
Abstract
Tailings ponds can recycle water resources through the water recirculation system by clarifying and purifying the wastewater discharged from the mining production process. Due to factors such as flooding and heavy rainfall, once a tailings dams burst, the spread of heavy metals in [...] Read more.
Tailings ponds can recycle water resources through the water recirculation system by clarifying and purifying the wastewater discharged from the mining production process. Due to factors such as flooding and heavy rainfall, once a tailings dams burst, the spread of heavy metals in the tailings causes underground and surface water pollution, endangering the lives and properties of people downstream. To effectively assess the potential impact of tailings dams bursting, many problems such as the difficulty of taking values in predicting the volume of silt penetration through empirical formulae, model testing, and numerical simulation need to be solved. In this study, 65 engineering cases were collected to develop a sample dataset containing dam height and storage capacity. The Support Vector Machine (SVM) algorithm was used to develop a nonlinear regression model for tailings discharge volume after tailings dam failure. In addition, the model penalty parameter C and kernel function g were optimized using the powerful global search capability of the Slime Mold Algorithm (SMA) to develop an SMA–SVM prediction model for tailings discharge volume. The results indicate that the volume of tailings discharged increases nonlinearly with increasing dam height and tailings storage capacity. The SMA-SVM model showed higher prediction accuracy compared to the predictions made by the Random Forest (RF), Radial Basis Function (RBF), and Least Squares SVM (LS-SVM) algorithms. The average absolute error in tailings discharge volume compared to actual values was 30,000 m3, with an average relative error of less than 25%. This is very close to practical engineering scenarios. The ability of the SMA-SVM optimization algorithm to produce predictions with minimal error relative to actual values was further confirmed by the combination of numerical simulations. In addition, the numerical simulations revealed the flow characteristics and inundation area of the discharged sediment during tailings dam failure, and the research results can provide reference for water resource protection and downstream safety prevention and control of tailings ponds. Full article
(This article belongs to the Section Hydraulics and Hydrodynamics)
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<p>Principle of the SVM Algorithm.</p>
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<p>SMA optimization process.</p>
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<p>Flowchart of the SMA-optimized SVM algorithm.</p>
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<p>Adaptation curve.</p>
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<p>Absolute error of prediction results for tailings discharge volume.</p>
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<p>Predicted results from different algorithms.</p>
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<p>Error analysis of prediction results from SMA-SVM algorithm.</p>
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<p>Numerical simulation model of overtopping dam failure (Dam height: 120.5 m).</p>
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<p>Influence range of tailings dam failure for different scenarios.</p>
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<p>Flow velocity at monitoring point M1 for different dam-failure scenarios.</p>
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<p>Flow velocity at monitoring point M2 for different dam-failure scenarios.</p>
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<p>Flow velocity at monitoring point M3 for different dam-failure scenarios.</p>
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<p>Flow velocity at monitoring point M4 for different dam-failure scenarios.</p>
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<p>Flow velocity at monitoring point M5 for different dam-failure scenarios.</p>
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<p>Flow velocity at monitoring point M6 for different dam-failure scenarios.</p>
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33 pages, 18193 KiB  
Article
Research on Traversal Path Planning and Collaborative Scheduling for Corn Harvesting and Transportation in Hilly Areas Based on Dijkstra’s Algorithm and Improved Harris Hawk Optimization
by Huanyu Liu, Jiahao Luo, Lihan Zhang, Hao Yu, Xiangnan Liu and Shuang Wang
Agriculture 2025, 15(3), 233; https://doi.org/10.3390/agriculture15030233 - 22 Jan 2025
Viewed by 697
Abstract
This study addresses the challenges of long traversal paths, low efficiency, high fuel consumption, and costs in the collaborative harvesting of corn by harvesters and grain transport vehicles in hilly areas. A path-planning and collaborative scheduling method is proposed, combining Dijkstra’s algorithm with [...] Read more.
This study addresses the challenges of long traversal paths, low efficiency, high fuel consumption, and costs in the collaborative harvesting of corn by harvesters and grain transport vehicles in hilly areas. A path-planning and collaborative scheduling method is proposed, combining Dijkstra’s algorithm with the Improved Harris Hawk Optimization (IHHO) algorithm. A field model based on Digital Elevation Model (DEM) data is created for full coverage path planning, reducing traversal path length. A field transfer road network is established, and Dijkstra’s algorithm is used to calculate distances between fields. A multi-objective collaborative scheduling model is then developed to minimize fuel consumption, scheduling costs, and time. The IHHO algorithm enhances search performance by introducing quantum initialization to improve the initial population, integrating the slime mold algorithm for better exploration, and applying an average differential mutation strategy and nonlinear energy factor updates to strengthen both global and local search. Non-dominated sorting and crowding distance techniques are incorporated to enhance solution diversity and quality. The results show that compared to traditional HHO and HHO algorithms, the IHHO algorithm reduces average scheduling costs by 4.2% and 14.5%, scheduling time by 4.5% and 8.1%, and fuel consumption by 3.5% and 3.2%, respectively. This approach effectively reduces transfer path costs, saves energy, and improves operational efficiency, providing valuable insights for path planning and collaborative scheduling in multi-field harvesting and transportation in hilly areas. Full article
(This article belongs to the Special Issue New Energy-Powered Agricultural Machinery and Equipment)
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<p>Geometric method for constructing field simulation model.</p>
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<p>Full coverage path planning for harvester field operations.</p>
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<p>Road network graph design.</p>
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<p>Weighted undirected graph.</p>
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<p>Distance matrix.</p>
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<p>Modification of the shortest path.</p>
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<p>Schematic diagram of field collaborative scheduling process.</p>
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<p>Technical roadmap.</p>
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<p>E behaviour before and after improvements.</p>
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<p>Behavior of El during two runs and 1000 iterations.</p>
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<p>The field operation path of corn harvester 1 is A → 1 → 11 → 6 → 17 → 18 → 19 → 12 → 16 → A.</p>
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<p>The field operation path of corn harvester 2 is A → 13 → 20 → 14 → 15 → A.</p>
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<p>The field operation path of corn harvester 3 is A → 3 → 2 → 7 → 8 → 4 → 9 → 5 → 10 → A.</p>
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<p>The field scheduling path of grain transporter 1 is A → 3 → 11 → 8 → 20 → 19 → 12 → A.</p>
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<p>The field scheduling path of grain transporter 2 is A → 1 → 7 → 17 → 18 → 14 → A → 10 → 16 → A.</p>
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<p>The field scheduling path of grain transporter 3 is A → 2 → 13 → 6 → 4 → A → 9 → 5 → 15 → A.</p>
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<p>The Gantt chart comparison between corn harvesters and grain transporters.</p>
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<p>Gantt chart of the HHO algorithm.</p>
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<p>Gantt chart from the literature [<a href="#B32-agriculture-15-00233" class="html-bibr">32</a>].</p>
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<p>(<b>a</b>–<b>c</b>) Comparison of objective functions of the three algorithms. Li, C. (2024) denotes literature [<a href="#B32-agriculture-15-00233" class="html-bibr">32</a>].</p>
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<p>Comparison of the cost box plots. Li, C(2024) denotes literature [<a href="#B32-agriculture-15-00233" class="html-bibr">32</a>].</p>
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<p>Comparison of the time box plots. Li, C(2024) denotes literature [<a href="#B32-agriculture-15-00233" class="html-bibr">32</a>].</p>
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<p>Comparison of the fuel consumption box plots. Li, C(2024) denotes literature [<a href="#B32-agriculture-15-00233" class="html-bibr">32</a>].</p>
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23 pages, 3967 KiB  
Article
Distribution and Diversity of Myxomycetes Along the Elevational Belt of Mt. Calavite Wildlife Sanctuary (MCWS), Occidental Mindoro, Philippines
by Christon Jairus M. Racoma, John Carlo Redeña-Santos and Nikki Heherson A. Dagamac
Ecologies 2025, 6(1), 7; https://doi.org/10.3390/ecologies6010007 - 17 Jan 2025
Viewed by 800
Abstract
Myxomycetes are protists that predate microbial communities in soil and are heavily affected by changing climate conditions. As seen in a more distinct guild of myxomycete, their fructification diversity depends not only on the heterogeneity of vegetation but also on temperature and precipitation. [...] Read more.
Myxomycetes are protists that predate microbial communities in soil and are heavily affected by changing climate conditions. As seen in a more distinct guild of myxomycete, their fructification diversity depends not only on the heterogeneity of vegetation but also on temperature and precipitation. To determine the reverse pattern of microbial diversity established in temperate ecozones, foliar and lignicolous litters were collected along a tropical montane site in the Philippines. Fifty-seven (57) morphospecies of myxomycetes from 15 genera were determined. Alpha-diversity analysis revealed a significant decline in species richness and diversity with increasing elevation. Beta-diversity analysis, integrating non-metric multidimensional scaling (NMDS), PERMANOVA, and hierarchical clustering, revealed the complex relationships between species turnover and community composition across elevational gradients. These results conform to the hypothesis that species richness decreases as elevation increases, supporting that tropical ecozones follow the general trend of myxomycete diversity that was first observed in the temperate ecozones. The strong role of elevation in shaping myxomycete community structure is further emphasized. This indicates that conservation management efforts should become more stringent in the areas found at the lower elevation of a tropical montane forest, which are more ecologically sensitive to human-induced stressors and climate-related pressures. Full article
(This article belongs to the Special Issue Feature Papers of Ecologies 2024)
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<p>Study area map of Mount Calavite Wildlife Sanctuary, Occidental Mindoro, showing the elevational gradient and the sampling points collected during the wet season in the months of August to October and the dry season in the months of March to May.</p>
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<p>Species accumulation curves (SACs) of myxomycetes composition on MWCS across different (<b>a</b>) seasonal collections, (<b>b</b>) substrate types, and (<b>c</b>) elevational gradients. Shaded areas indicate confidence intervals (conf = 0.95) for the estimates. Sampling completeness—seasonal collection: dry (63.43%) and wet (54.83%); substrate types: aerial (38.16%), ground (61.10%), and wood (42.83%); elevational gradient: elevation 1 (23.22%), elevation 2 (39.37%), elevation 3 (70.86%), elevation 4 (73.18%), and elevation five (95.02%).</p>
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<p>Rank abundance curve plots based on the abundance of species for different substrate types (aerial, ground, and wood), seasons (wet and dry), and elevational ranges (elevations 1, 2, 3, 4, and 5) testing five distribution models; thickened line represents best-fitting curve—Zipf model: shows how few species dominate in abundance, while many are rare; Mandelbrot model: shows where dominant species flatten in abundance and rare species decline sharply [<a href="#B51-ecologies-06-00007" class="html-bibr">51</a>]; Preemption model: competitive species dominate initially due to abiotic factors, while subsequent species’ abundances are influenced by competition [<a href="#B52-ecologies-06-00007" class="html-bibr">52</a>,<a href="#B53-ecologies-06-00007" class="html-bibr">53</a>].</p>
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<p>Alpha-diversity violin plots showing the comparison of four different diversity indices (Shannon’s index; Simpson’s index; Shannon Exponential; Inverse Simpson) in relation to season (<b>left column</b>), substrate type (<b>middle column</b>), and elevational range (<b>right column</b>).</p>
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<p>Non-metric multidimensional scaling (NMDS) ordination plot of myxomycetes species composition across different seasons, substrate types, and elevational ranges; ellipses represent 95% confidence intervals around per group; stress plot showing the relationship between observed dissimilarities and ordination distances from the NMDS analysis (k = 2); red line shows a smoothed fit, non-metric fit (R<sup>2</sup> = 0.976), and linear fit (R<sup>2</sup> = 0.93).</p>
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<p>Neighbor-joining clustering tree based on species composition (species and abundances) utilizing Bray–Curtis dissimilarity (CCC = 0.9678) and Jaccard similarity distances (CCC = 0.8226); Monte-Carlo plot simulating the hypotheses—species decreases as elevation increases—and mid-domain hypothesis.</p>
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12 pages, 4669 KiB  
Article
Metagenomic Insight into the Associated Microbiome in Plasmodia of Myxomycetes
by Xueyan Peng, Shu Li, Wenjun Dou, Mingxin Li, Andrey A. Gontcharov, Zhanwu Peng, Bao Qi, Qi Wang and Yu Li
Microorganisms 2024, 12(12), 2540; https://doi.org/10.3390/microorganisms12122540 - 10 Dec 2024
Viewed by 799
Abstract
During the trophic period of myxomycetes, the plasmodia of myxomycetes can perform crawling feeding and phagocytosis of bacteria, fungi, and organic matter. Culture-based studies have suggested that plasmodia are associated with one or several species of bacteria; however, by amplicon sequencing, it was [...] Read more.
During the trophic period of myxomycetes, the plasmodia of myxomycetes can perform crawling feeding and phagocytosis of bacteria, fungi, and organic matter. Culture-based studies have suggested that plasmodia are associated with one or several species of bacteria; however, by amplicon sequencing, it was shown that up to 31–52 bacteria species could be detected in one myxomycete, suggesting that the bacterial diversity associated with myxomycetes was likely to be underestimated. To fill this gap and characterize myxomycetes’ microbiota and functional traits, the diversity and functional characteristics of microbiota associated with the plasmodia of six myxomycetes species were investigated by metagenomic sequencing. The results indicate that the plasmodia harbored diverse microbial communities, including eukaryotes, viruses, archaea, and the dominant bacteria. The associated microbiomes represented more than 22.27% of the plasmodia genome, suggesting that these microbes may not merely be parasitic or present as food but rather may play functional roles within the plasmodium. The six myxomycetes contained similar bacteria, but the bacteria community compositions in each myxomycete were species-specific. Functional analysis revealed a highly conserved microbial functional profile across the six plasmodia, suggesting they may serve a specific function for the myxomycetes. While the host-specific selection may shape the microbial community compositions within plasmodia, functional redundancy ensures functional stability across different myxomycetes. Full article
(This article belongs to the Section Microbiomes)
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<p>Six plasmodia of myxomycetes cultured on water agar media: phaneroplasmodium (<b>a</b>) <span class="html-italic">D. squamulosum</span>, (<b>b</b>) <span class="html-italic">D. nigripes</span>, (<b>c</b>) <span class="html-italic">F. gyrosa</span>, (<b>d</b>) <span class="html-italic">B. melanospora</span>, and aphanoplasmodium (<b>e</b>) <span class="html-italic">A. cinerea</span>, (<b>f</b>) <span class="html-italic">M. scintillans</span>.</p>
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<p>Relative abundance of microbial communities in each plasmodium. The domain level (<b>a</b>) and top 15 phylum level (<b>b</b>) are displayed at community compositions. Phylum outside the top 15 samples was assigned as “Others”.</p>
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<p>Bacterial community composition and diversity analysis of each plasmodium. (<b>a</b>) Hierarchical clustering analysis (weighted Unifrac UPGMA) and relative abundance of bacterial communities associated with each plasmodium and Venn diagrams show the number and abundance of shared and unique bacteria in each plasmodium at the genus level. (<b>b</b>) The α diversity (Chao, Shannon index, and Pielou_e) and (<b>c</b>) PCoA analysis using the Bray–Curtis distance metric showed the plasmodia bacterial communities’ diversity. (<b>d</b>) Relative abundances of the top 10 phylum levels of bacterial communities. (<b>e</b>) Venn diagram shows the shared and unique bacteria at the species level in each plasmodia sample. The differences were considered significant when <span class="html-italic">p</span> values &lt; 0.05. *: <span class="html-italic">p</span> &lt; 0.05.</p>
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<p>Relative abundance of Gram-positive/Gram-negative bacteria in each plasmodium.</p>
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<p>Functional analysis of plasmodia-associated bacteria. (<b>a</b>) A comparison of the top 25 COG functional categories in the six plasmodia. (<b>b</b>) Gene count and relative abundance of CAZy class categories. (<b>c</b>) Functional KEGG level 1 and (<b>d</b>) KEGG level 2 pathway descriptions, and (<b>e</b>) relative abundance of top 20 KEGG level 3 pathway categories.</p>
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<p>Composition and functional analysis of probiotics in six myxomycetes. Relative abundance of probiotics at genus level (<b>a</b>) and top 25 of functional composition of probiotics (<b>b</b>) was exhibited in each plasmodium.</p>
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23 pages, 8896 KiB  
Article
New Species and Records Expand the Checklist of Cellular Slime Molds (Dictyostelids) in Jilin Province, China
by Zhaojuan Zhang, Liang He, Yuqing Sun, Zhuang Li, Yingkun Yang, Chao Zhai, Steven L. Stephenson, Xiangrui Xie, Yu Li and Pu Liu
J. Fungi 2024, 10(12), 834; https://doi.org/10.3390/jof10120834 - 2 Dec 2024
Viewed by 775
Abstract
Dictyostelids represent a crucial element in the protist community, and their abundant presence in Jilin Province underscores their indispensable role in biodiversity conservation. In the present study, a resource survey of dictyostelids used random sampling to collect 28 soil samples from five localities [...] Read more.
Dictyostelids represent a crucial element in the protist community, and their abundant presence in Jilin Province underscores their indispensable role in biodiversity conservation. In the present study, a resource survey of dictyostelids used random sampling to collect 28 soil samples from five localities in Changbai Korean Autonomous County, Jilin Province. In addition, a compilation of dictyostelid species reported from Jilin Province was developed, based on a thorough review of the literature. The survey yielded fifteen isolates of dictyostelids, comprising six species from four genera. Notably, two new species (Dictyostelium longigracilis sp. nov. and Dictyostelium macrosoriobrevipes sp. nov.) were described using morphological characteristics and SSU gene-based phylogenetic analyses. One other species (Polysphondylium patagonicum) was recorded as new for China, while another (Cavenderia aureostipes) was recorded as a new record for Jilin Province. The dictyostelid assemblage in Jilin Province is dominated by the genus Dictyostelium (51.4%), with a total of 35 species, which represent 59.3% of the current total known for all of China. These findings provide a scientific basis for the protection of species diversity and resource utilization of dictyostelids in Jilin Province. Full article
(This article belongs to the Special Issue Fungal Communities in Various Environments)
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<p>Study area and location of the sampling sites in Changbai Korean Autonomous County, Jilin Province, China (<b>a</b>). Outline of the experimental workflow used to isolate dictyostelids from the collected soil samples (<b>b</b>), the arrow indicates the step-by-step progression of the experiment. Note: the map was drawn using ArcGIS 10.8 [<a href="#B28-jof-10-00834" class="html-bibr">28</a>] software. Details of the samples (e.g., sample location, number of soil samples, and vegetation types) are shown in <a href="#jof-10-00834-t001" class="html-table">Table 1</a>.</p>
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<p>Morphological features of <span class="html-italic">Dictyostelium longigracilis</span>: (<b>a</b>,<b>b</b>) sorocarps; (<b>c</b>) aggregation; (<b>d</b>,<b>e</b>) pseudoplasmodia; (<b>f</b>) spores; (<b>g</b>) sorophores; (<b>h</b>,<b>i</b>) sorophore tips; and (<b>j</b>) sorophore base. Scale bars: (<b>a</b>–<b>c</b>) = 2 mm; (<b>d</b>,<b>e</b>) = 1 mm; (<b>f</b>,<b>i</b>,<b>j</b>) = 20 μm; (<b>g</b>) = 50 μm; and (<b>h</b>) = 10 μm. The red arrows refer to the key distinguishing characteristics.</p>
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<p>Morphological features of <span class="html-italic">Dictyostelium macrosoriobrevipes</span>: (<b>a</b>–<b>c</b>) sorocarps; (<b>d</b>) aggregations; (<b>e</b>) pseudoplasmodia; (<b>f</b>) sorogens; (<b>g</b>) spores; (<b>h</b>,<b>i</b>) sorophores; (<b>j</b>) tip of sorophore; and (<b>k</b>) base of sorophore. Scale bars: (<b>a</b>,<b>c</b>) = 2 mm; (<b>b</b>) = 200 μm; (<b>d</b>,<b>f</b>) = 500 μm; (<b>e</b>) = 1 mm; (<b>g</b>–<b>j</b>) = 50 μm; and (<b>k</b>) = 20 μm. The red arrows refer to the key distinguishing characteristics.</p>
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<p>Morphological features of <span class="html-italic">Dictyostelium robusticaule</span>: (<b>a</b>) sorocarps; (<b>b</b>) aggregations; (<b>c</b>) pseudoplasmodia; (<b>d</b>) spores; (<b>e</b>) sorophore; (<b>f</b>) sorophore tip; and (<b>g</b>) sorophore base. Scale bars: (<b>a</b>) = 2 mm; (<b>b</b>,<b>c</b>) = 1 mm; and (<b>d</b>–<b>g</b>) = 20 μm. The red arrows refer to the key distinguishing characteristics.</p>
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<p>Morphological features of <span class="html-italic">Polysphondylium patagonicum</span>: (<b>a</b>,<b>b</b>) sorocarps; (<b>c</b>,<b>d</b>) aggregations; (<b>e</b>) pseudoplasmodia; (<b>f</b>) sorophore; (<b>g</b>) branches; (<b>h</b>) spores; (<b>i</b>) sorophore tip; and (<b>j</b>) sorophore base. Scale bars: (<b>a</b>,<b>c</b>) = 1 mm; (<b>b</b>,<b>e</b>) = 500 μm; (<b>d</b>) =2 mm; and (<b>f</b>–<b>j</b>) = 20 μm. The red arrows refer to the key distinguishing characteristics.</p>
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<p>Morphological features of <span class="html-italic">Heterostelium candidum</span>: (<b>a</b>) sorocarps; (<b>b</b>) aggregations; (<b>c</b>) pseudoplasmodia; (<b>d</b>) spores; (<b>e</b>) sorophore; (<b>f</b>) sorophore tip; and (<b>g</b>) sorophore base. Scale bars: (<b>a</b>,<b>b</b>) = 1 mm; (<b>c</b>) = 500 μm; (<b>d</b>) = 20 μm; (<b>e</b>) = 10 μm; (<b>f</b>) = 50 μm; and (<b>g</b>) = 20 μm. The red arrows refer to the key distinguishing characteristics.</p>
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<p>Morphological features of <span class="html-italic">Cavenderia aureostipes</span>: (<b>a</b>) sorocarps; (<b>b</b>) aggregations; (<b>c</b>) pseudoplasmodia; (<b>d</b>) spores; (<b>e</b>) sorophore tip; (<b>f</b>) sorophore; and (<b>g</b>) sorophore base. Scale bars: (<b>a</b>,<b>c</b>) = 1 mm; (<b>b</b>) = 500 μm; and (<b>d</b>–<b>g</b>) = 20 μm. The red arrows refer to the key distinguishing characteristics.</p>
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<p>Phylogeny of all known species of dictyostelids based on SSU rDNA. Newly generated sequences are indicated in red.</p>
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<p>SSU phylogeny of <span class="html-italic">Dictyostelium</span> (<b>a</b>) and <span class="html-italic">Polysphondylium</span> (<b>b</b>) sequences in the order Dictyosteliales and the family Dictyosteliaceae. Numbers in parentheses are SH-aLRT support (%)/ultrafast bootstrap support (%). Newly generated sequences are indicated in red and the new species are framed in black.</p>
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<p>SSU phylogeny of <span class="html-italic">Heterostelium</span> sequences in the order Acytosteliales and the family Acytosteliaceae. Numbers in parentheses are SH-aLRT support (%)/ultrafast bootstrap support (%). Newly generated sequences are indicated in red.</p>
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<p>SSU phylogeny of <span class="html-italic">Cavenderia</span> sequences in the order Acytosteliales and the family Cavenderiaceae. Numbers in parentheses are SH-aLRT support (%)/ultrafast bootstrap support (%). Newly generated sequences are indicated in red.</p>
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<p>The new classification as indicated by nuclear small subunit (18S) [<a href="#B25-jof-10-00834" class="html-bibr">25</a>] rDNA sequences, displayed with a black border (<b>a</b>) and numbers of dictyostelid species within the order (<b>b</b>), family (<b>c</b>), and genus (<b>d</b>) in Jilin Province, China.</p>
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15 pages, 2833 KiB  
Article
Morphological and Phylogenetic Analyses Reveal Dictyostelids (Cellular Slime Molds) Colonizing the Ascocarp of Morchella
by Wen-Shu Hu, Lin-Lin Jiang, Pu Liu, Xiao-Yan Zhang, Wei Wei and Xi-Hui Du
J. Fungi 2024, 10(10), 678; https://doi.org/10.3390/jof10100678 - 28 Sep 2024
Viewed by 1196
Abstract
Morchella spp. (true morels) are precious edible mushrooms consumed around the world, with a delicious taste, rich nutritional value, and unique healthcare effects. Various fungi and bacteria have been reported to colonize the ascocarps of Morchella, damaging their fruiting bodies and leading [...] Read more.
Morchella spp. (true morels) are precious edible mushrooms consumed around the world, with a delicious taste, rich nutritional value, and unique healthcare effects. Various fungi and bacteria have been reported to colonize the ascocarps of Morchella, damaging their fruiting bodies and leading to serious economic losses in cultivation. The species identification of these colonizing organisms is crucial for understanding their colonization mechanisms on morels. Slime molds, which have characteristics of both “fungi” and “animals”, can occasionally colonize crops and edible fungi. However, there have been no reports of dictyostelid cellular slime molds (dictyostelids) colonizing plants and fungi to date. In this study, we discovered that dictyostelids colonized the surface of one wild ascoma of Morchella in the forest of Chongqing, China, with the tissues being black and rotten. Macro- and micro-morphological observations, along with molecular phylogenetic analyses, identified the specimens investigated in this study as Dictyostelium implicatum and Morchella sp. Mel-21. The results provide new knowledge of dictyostelid colonization on organisms and contribute to the diversity of species colonizing true morels. Moreover, this is also the first report of dictyostelids distributed in Chongqing, China. This study enhances our insights into the life history and potential ecological significance of dictyostelids and updates their distribution area in China. Further research will be conducted to uncover the mechanisms behind the colonization observed in this study. Full article
(This article belongs to the Special Issue Diversity, Phylogeny and Ecology of Forest Fungi)
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<p>Slime molds colonizing the ascoma of <span class="html-italic">Morchella</span> in the field. (<b>A</b>) Distant view; (<b>B</b>,<b>C</b>) close-up view. Slime molds indicated by white arrows.</p>
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<p>The phylogenetic tree of 28 <span class="html-italic">Morchella</span> species inferred from ML analyses based on the concatenated dataset (ITS, <span class="html-italic">EF1-a</span>, <span class="html-italic">RPB1</span>, and <span class="html-italic">RPB2</span>). Bootstrap values over 75% and Bayesian posterior probabilities over 0.95 shown on the branches. The new specimen of <span class="html-italic">Morchella</span> used in this study indicated in bold.</p>
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<p>Morphological characteristics of <span class="html-italic">Dictyostelium</span> sp. investigated in this study under a stereo microscope. (<b>A</b>) Cell aggregation; (<b>B</b>) pseudoplasmodium; (<b>C</b>–<b>E</b>) mexican-hat-like protrusion; (<b>F</b>–<b>H</b>) the sorocarp formation period with mastoid structure; (<b>I</b>) sorocarps. Scale bars = 200 μm.</p>
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<p>Microscopic morphological characteristics of <span class="html-italic">Dictyostelium</span> sp. observed under a light microscope. (<b>A</b>) Spores; (<b>B</b>) spore germination; (<b>C</b>,<b>D</b>) microcysts; (<b>E</b>) base of sorophores; (<b>F</b>) tip of sorophores. Scale bars = 5 μm.</p>
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<p>The phylogenetic tree of 57 species of dictyostelids inferred from ML analyses based on 18S rRNA. Bootstrap values over 75% and Bayesian posterior probabilities over 0.95 reported on the branches. The new collection of <span class="html-italic">Dictyostelium</span> used in this study indicated in bold.</p>
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19 pages, 1460 KiB  
Article
Azimuthal Solar Synchronization and Aerodynamic Neuro-Optimization: An Empirical Study on Slime-Mold-Inspired Neural Networks for Solar UAV Range Optimization
by Graheeth Hazare, Mohamed Thariq Hameed Sultan, Dariusz Mika, Farah Syazwani Shahar, Grzegorz Skorulski, Marek Nowakowski, Andriy Holovatyy, Ile Mircheski and Wojciech Giernacki
Appl. Sci. 2024, 14(18), 8265; https://doi.org/10.3390/app14188265 - 13 Sep 2024
Viewed by 1009
Abstract
This study introduces a novel methodology for enhancing the efficiency of solar-powered unmanned aerial vehicles (UAVs) through azimuthal solar synchronization and aerodynamic neuro-optimization, leveraging the principles of slime mold neural networks. The objective is to broaden the operational capabilities of solar UAVs, enabling [...] Read more.
This study introduces a novel methodology for enhancing the efficiency of solar-powered unmanned aerial vehicles (UAVs) through azimuthal solar synchronization and aerodynamic neuro-optimization, leveraging the principles of slime mold neural networks. The objective is to broaden the operational capabilities of solar UAVs, enabling them to perform over extended ranges and in varied weather conditions. Our approach integrates a computational model of slime mold networks with a simulation environment to optimize both the solar energy collection and the aerodynamic performance of UAVs. Specifically, we focus on improving the UAVs’ aerodynamic efficiency in flight, aligning it with energy optimization strategies to ensure sustained operation. The findings demonstrated significant improvements in the UAVs’ range and weather resilience, thereby enhancing their utility for a variety of missions, including environmental monitoring and search and rescue operations. These advancements underscore the potential of integrating biomimicry and neural-network-based optimization in expanding the functional scope of solar UAVs. Full article
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<p>Process flow charts. (<b>a</b>) The neural network optimization process using Slime Mold Optimization (SMO). (<b>b</b>) The environmental interaction process for terrain navigation and obstacle avoidance.</p>
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<p>Neural network layout.</p>
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<p>VLM discretization scheme (x, y, z in meters).</p>
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<p>Optimization results: (<b>a</b>) exploration vs. exploitation and (<b>b</b>) runtime.</p>
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19 pages, 2944 KiB  
Article
Gradient Boosting Regression Tree Optimized with Slime Mould Algorithm to Predict the Higher Heating Value of Municipal Solid Waste
by Esraa Q. Shehab, Farah Faaq Taha, Sabih Hashim Muhodir, Hamza Imran, Krzysztof Adam Ostrowski and Marcin Piechaczek
Energies 2024, 17(17), 4213; https://doi.org/10.3390/en17174213 - 23 Aug 2024
Cited by 1 | Viewed by 970
Abstract
The production of municipal solid waste (MSW) has led to an unprecedented level of environmental pollution, worsening the global challenges posed by climate change. Researchers and policymakers have recently made significant strides in the field of sustainable and renewable energy sources, which are [...] Read more.
The production of municipal solid waste (MSW) has led to an unprecedented level of environmental pollution, worsening the global challenges posed by climate change. Researchers and policymakers have recently made significant strides in the field of sustainable and renewable energy sources, which are viable from technological, environmental, and economic perspectives. Consequently, the waste-to-energy programs enhance nations’ socioeconomic status while positively impacting the environment. To predict the higher heating value (HHV) of MSW fuel based on carbon, hydrogen, oxygen, nitrogen, and sulfur content, the current study introduces a Gradient Boosting Regression Tree (GBRT) model optimized with the Slime Mold Algorithm (SMA). This model was evaluated using an additional 50 data points after being trained with 202 MSW biomass data points. The performance of the model was assessed using three metrics: root mean square error (RMSE), mean absolute error (MAE), and the coefficient of determination (R2). The results indicated that our model outperformed previously developed models in terms of accuracy and reliability. Additionally, a graphical user interface (GUI) was developed to facilitate the practical application of the model, allowing users to easily input data and receive predictions on the enthalpy of the combustion of MSW fuel. Full article
(This article belongs to the Special Issue Advances in Renewable Energy Power Forecasting and Integration)
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<p>Global treatment and disposal of waste (percent) [<a href="#B16-energies-17-04213" class="html-bibr">16</a>].</p>
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<p>Visualization of the incidences of individual keywords and their relationships (accessed on 20 June 2024).</p>
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<p>Research methodology.</p>
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<p>Workflow of Gradient Boosting Regression Trees (GBRTs).</p>
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<p>Pearson correlations between input and output variables.</p>
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<p>Five-Fold cross-validation RMSE results for the SMA-GBRT model.</p>
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<p>The approximate relationship between the actual and predicted (<b>a</b>) training set and (<b>b</b>) testing set.</p>
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<p>Scatter plots of the actual and predicted (<b>a</b>) training set and (<b>b</b>) testing set.</p>
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<p>Performance comparison between the model developed in this study and previously developed models for (<b>a</b>) the training dataset, (<b>b</b>) the testing dataset, and (<b>c</b>) the entire dataset.</p>
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<p>Residual normal distribution for our model and previously developed model: (<b>a</b>) training; (<b>b</b>) testing; and (<b>c</b>) all data.</p>
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18 pages, 2392 KiB  
Article
Robot Motion Planning Based on an Adaptive Slime Mold Algorithm and Motion Constraints
by Rong Chen, Huashan Song, Ling Zheng and Bo Wang
World Electr. Veh. J. 2024, 15(7), 296; https://doi.org/10.3390/wevj15070296 - 3 Jul 2024
Cited by 2 | Viewed by 1067
Abstract
The rapid advancement of artificial intelligence technology has significantly enhanced the intelligence of mobile robots, facilitating their widespread utilization in unmanned driving, smart home systems, and various other domains. As the scope, scale, and complexity of robot deployment continue to expand, there arises [...] Read more.
The rapid advancement of artificial intelligence technology has significantly enhanced the intelligence of mobile robots, facilitating their widespread utilization in unmanned driving, smart home systems, and various other domains. As the scope, scale, and complexity of robot deployment continue to expand, there arises a heightened demand for enhanced computational power and real-time performance, with path planning emerging as a prominent research focus. In this study, we present an adaptive Lévy flight–rotation slime mold algorithm (LRSMA) for global robot motion planning, which incorporates LRSMA with the cubic Hermite interpolation. Unlike traditional methods, the algorithm eliminates the need for a priori knowledge of appropriate interpolation points. Instead, it autonomously detects the convergence status of LRSMA, dynamically increasing interpolation points to enhance the curvature of the motion curve when it surpasses the predefined threshold. Subsequently, it compares path lengths resulting from two different objective functions to determine the optimal number of interpolation points and the best path. Compared to LRSMA, this algorithm reduced the minimum path length and average processing time by (2.52%, 3.56%) and (38.89%, 62.46%), respectively, along with minimum processing times. Our findings demonstrate that this method effectively generates collision-free, smooth, and curvature-constrained motion curves with the least processing time. Full article
(This article belongs to the Special Issue Design Theory, Method and Control of Intelligent and Safe Vehicles)
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<p>The flow chart of the Lévy flight–rotation slime mold algorithm (LRSMA).</p>
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<p>The flow chart of path planning based on an adaptive LRSMA.</p>
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<p>The optimal path generated by two algorithms in scenario 1: (<b>a</b>) combining cubic B-spline interpolation with LRSMA; (<b>b</b>) combining cubic B-spline interpolation with LRSMA; (<b>c</b>) combining cubic Hermite interpolation with adaptive LRSMA.</p>
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<p>The optimal path generated by two algorithms in scenario 2: (<b>a</b>) combining cubic B-spline interpolation with LRSMA; (<b>b</b>) combining cubic B-spline interpolation with LRSMA; (<b>c</b>) combining cubic Hermite interpolation with adaptive LRSMA.</p>
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<p>Comparison of iterative curves generated by the algorithm combining cubic Hermite interpolation with adaptive LRSMA: (<b>a</b>) the iterative curve in scenario 1; and (<b>b</b>) the iterative curve in scenario 2.</p>
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23 pages, 2335 KiB  
Article
Enhanced Target Localization in the Internet of Underwater Things through Quantum-Behaved Metaheuristic Optimization with Multi-Strategy Integration
by Xiaojun Mei, Fahui Miao, Weijun Wang, Huafeng Wu, Bing Han, Zhongdai Wu, Xinqiang Chen, Jiangfeng Xian, Yuanyuan Zhang and Yining Zang
J. Mar. Sci. Eng. 2024, 12(6), 1024; https://doi.org/10.3390/jmse12061024 - 19 Jun 2024
Cited by 2 | Viewed by 1372
Abstract
Underwater localization is considered a critical technique in the Internet of Underwater Things (IoUTs). However, acquiring accurate location information is challenging due to the heterogeneous underwater environment and the hostile propagation of acoustic signals, especially when using received signal strength (RSS)-based techniques. Additionally, [...] Read more.
Underwater localization is considered a critical technique in the Internet of Underwater Things (IoUTs). However, acquiring accurate location information is challenging due to the heterogeneous underwater environment and the hostile propagation of acoustic signals, especially when using received signal strength (RSS)-based techniques. Additionally, most current solutions rely on strict mathematical expressions, which limits their effectiveness in certain scenarios. To address these challenges, this study develops a quantum-behaved meta-heuristic algorithm, called quantum enhanced Harris hawks optimization (QEHHO), to solve the localization problem without requiring strict mathematical assumptions. The algorithm builds on the original Harris hawks optimization (HHO) by integrating four strategies into various phases to avoid local minima. The initiation phase incorporates good point set theory and quantum computing to enhance the population quality, while a random nonlinear technique is introduced in the transition phase to expand the exploration region in the early stages. A correction mechanism and exploration enhancement combining the slime mold algorithm (SMA) and quasi-oppositional learning (QOL) are further developed to find an optimal solution. Furthermore, the RSS-based Cramér–Raolower bound (CRLB) is derived to evaluate the effectiveness of QEHHO. Simulation results demonstrate the superior performance of QEHHO under various conditions compared to other state-of-the-art closed-form-expression- and meta-heuristic-based solutions. Full article
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<p>The stratified propagation of the acoustic signal.</p>
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<p>Flowchart of underwater localization using QEHHO.</p>
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<p>RMSE versus variable <math display="inline"><semantics> <msub> <mi>Max</mi> <mi>t</mi> </msub> </semantics></math> with <math display="inline"><semantics> <mrow> <mi>N</mi> <mo>=</mo> <mn>8</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <mi>M</mi> <mo>=</mo> <mn>30</mn> </mrow> </semantics></math>, and <math display="inline"><semantics> <mrow> <msubsup> <mi>σ</mi> <mi>i</mi> <mn>2</mn> </msubsup> <mo>=</mo> <mn>3</mn> </mrow> </semantics></math> dB.</p>
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<p>CDF when <math display="inline"><semantics> <mrow> <msub> <mi>Max</mi> <mi>t</mi> </msub> <mo>=</mo> <mn>10</mn> </mrow> </semantics></math> with <math display="inline"><semantics> <mrow> <mi>N</mi> <mo>=</mo> <mn>8</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <mi>M</mi> <mo>=</mo> <mn>30</mn> </mrow> </semantics></math>, and <math display="inline"><semantics> <mrow> <msubsup> <mi>σ</mi> <mi>i</mi> <mn>2</mn> </msubsup> <mo>=</mo> <mn>3</mn> </mrow> </semantics></math> dB.</p>
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<p>RMSE versus variable <span class="html-italic">M</span> with <math display="inline"><semantics> <mrow> <mi>N</mi> <mo>=</mo> <mn>8</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>Max</mi> <mi>t</mi> </msub> <mo>=</mo> <mn>10</mn> </mrow> </semantics></math>, and <math display="inline"><semantics> <mrow> <msubsup> <mi>σ</mi> <mi>i</mi> <mn>2</mn> </msubsup> <mo>=</mo> <mn>3</mn> </mrow> </semantics></math> dB.</p>
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<p>CDF when <math display="inline"><semantics> <mrow> <mi>M</mi> <mo>=</mo> <mn>30</mn> </mrow> </semantics></math> with <math display="inline"><semantics> <mrow> <mi>N</mi> <mo>=</mo> <mn>8</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>Max</mi> <mi>t</mi> </msub> <mo>=</mo> <mn>10</mn> </mrow> </semantics></math>, and <math display="inline"><semantics> <mrow> <msubsup> <mi>σ</mi> <mi>i</mi> <mn>2</mn> </msubsup> <mo>=</mo> <mn>3</mn> </mrow> </semantics></math> dB.</p>
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<p>RMSE versus variable <span class="html-italic">N</span> with <math display="inline"><semantics> <mrow> <mi>M</mi> <mo>=</mo> <mn>30</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>Max</mi> <mi>t</mi> </msub> <mo>=</mo> <mn>10</mn> </mrow> </semantics></math>, and <math display="inline"><semantics> <mrow> <msubsup> <mi>σ</mi> <mi>i</mi> <mn>2</mn> </msubsup> <mo>=</mo> <mn>3</mn> </mrow> </semantics></math> dB.</p>
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<p>CDF when <math display="inline"><semantics> <mrow> <mi>N</mi> <mo>=</mo> <mn>12</mn> </mrow> </semantics></math> with <math display="inline"><semantics> <mrow> <mi>M</mi> <mo>=</mo> <mn>30</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>Max</mi> <mi>t</mi> </msub> <mo>=</mo> <mn>10</mn> </mrow> </semantics></math>, and <math display="inline"><semantics> <mrow> <msubsup> <mi>σ</mi> <mi>i</mi> <mn>2</mn> </msubsup> <mo>=</mo> <mn>3</mn> </mrow> </semantics></math> dB.</p>
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<p>RMSE versus variable <math display="inline"><semantics> <msubsup> <mi>σ</mi> <mi>i</mi> <mn>2</mn> </msubsup> </semantics></math> with <math display="inline"><semantics> <mrow> <mi>M</mi> <mo>=</mo> <mn>30</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>Max</mi> <mi>t</mi> </msub> <mo>=</mo> <mn>10</mn> </mrow> </semantics></math>, and <math display="inline"><semantics> <mrow> <mi>N</mi> <mo>=</mo> <mn>8</mn> </mrow> </semantics></math>.</p>
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<p>CDF when <math display="inline"><semantics> <mrow> <msubsup> <mi>σ</mi> <mi>i</mi> <mn>2</mn> </msubsup> <mo>=</mo> <mn>12</mn> </mrow> </semantics></math> dB with <math display="inline"><semantics> <mrow> <mi>M</mi> <mo>=</mo> <mn>30</mn> </mrow> </semantics></math>, <math display="inline"><semantics> <mrow> <msub> <mi>Max</mi> <mi>t</mi> </msub> <mo>=</mo> <mn>10</mn> </mrow> </semantics></math>, and <math display="inline"><semantics> <mrow> <mi>N</mi> <mo>=</mo> <mn>8</mn> </mrow> </semantics></math>.</p>
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<p>Computational time for different scenarios.</p>
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19 pages, 7985 KiB  
Article
Diversity of Cellular Slime Molds (Dictyostelids) in the Fanjing Mountain Nature Reserve and Geographical Distribution Comparisons with Other Representative Nature Reserves in Different Climate Zones of China
by Zhaojuan Zhang, Meng Li, Shufei Zhang, Yue Qin, Jing Zhao, Yu Li, Steven L. Stephenson, Junzhi Qiu and Pu Liu
Microorganisms 2024, 12(6), 1061; https://doi.org/10.3390/microorganisms12061061 - 24 May 2024
Cited by 2 | Viewed by 1191
Abstract
Protected areas are widely considered an essential strategy for biodiversity conservation. Dictyostelids are unique protists known to have important ecological functions in promoting soil and plant health through their top-down regulation of ecosystem processes, such as decomposition, that involve bacterial populations. But the [...] Read more.
Protected areas are widely considered an essential strategy for biodiversity conservation. Dictyostelids are unique protists known to have important ecological functions in promoting soil and plant health through their top-down regulation of ecosystem processes, such as decomposition, that involve bacterial populations. But the relationship between dictyostelid diversity within protected areas remains poorly understood, especially on a large scale. Herein, we report data on the distribution of dictyostelids, identified with ITS + SSU rRNA molecular and morphology-based taxonomy, from soil samples collected in the Fanjing Mountain protected area of Guizhou Province, Southwest China. We compared the biodiversity data of dictyostelids in Fanjing Mountain with similar data from previously sampled sites in four other protected areas, including Changbai Mountain (CB), Gushan Mountain (GS), Baiyun Mountain (BY), and Qinghai–Tibet Plateau (QT) in China. We identified four species of dictyostelids belonging to three genera (Dictyostelium, Heterostelium, and Polysphondylium) and herein provide information on the taxonomy of these species. Two species (Heterostelium pallidum and Dictyostelium purpureum) are common and widely distributed throughout the world, but one species (Polysphondylium fuscans) was new to China. Our data indicate that there is no distinguishable significant correlation between the dictyostelid species studied and environmental factors. Overall, the similarity index between Baiyun Mountain in Henan Province and Fanjing Mountain in Guizhou Province, located at approximately the same longitude, is the highest, and the Jaccard similarity coefficients (Jaccard index) of family, genus, and species are 100%, 100%, and 12.5%, respectively. From a species perspective, species in the same climate zone are not closely related, but obvious geographical distributions are evident in different climate zones. This preliminary study provided evidence of the ecological adaptation of dictyostelids to different biological niches. Full article
(This article belongs to the Section Environmental Microbiology)
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<p>Changes in the distribution of sampling sites used to assess dictyostelid communities in China and the life cycles of Amoebozoan groups related to the dictyostelids. (<b>A</b>) The dictyostelids show both asexual and sexual cycles. (<b>B</b>) The legend of “sampling sites” is based on a literature analysis (i.e., China National Knowledge Infrastructure, CNKI).</p>
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<p>Location of the Fanjing Mountain Nature Reserve in China. (<b>A</b>) Map of the Fanjing Mountain Nature Reserve in the northeast of Guizhou Province, China. (<b>B</b>) Study area (nine-point sampling method) used in the Fanjing Mountain Nature Reserve. (<b>C</b>) Image of the Fanjing Mountain Nature Reserve. (<b>D</b>) Mixed forest. (<b>E</b>) Broadleaf forest. (<b>F</b>) Coniferous forest.</p>
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<p>Outline of the experimental workflow used to isolate dictyostelids from the collected soil samples. (<b>A</b>) Sample processing. (<b>B</b>) Morphological observations. (<b>C</b>) DNA isolation, PCR amplification, sequencing.</p>
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<p>Comparison of the community diversity of dictyostelids along the different dimensional diversity levels. (<b>A</b>) Density in the plots. Abbreviations: broadleaf forest (BF), coniferous forest (CF), mixed forest (MF). (<b>B</b>) The relative abundance and frequency of species.</p>
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<p>Morphological features of <span class="html-italic">Polysphondylium fuscans</span> Perrigo and Romeralo (HMJAU A241). (<b>A</b>,<b>B</b>) Sorocarps. (<b>C</b>) Aggregations. (<b>D</b>) Pseudoplasmodia. (<b>E</b>) Clustered sorogens. (<b>F</b>) Spores. (<b>G</b>,<b>H</b>) Sorophore tips. (<b>I</b>,<b>J</b>) Sorophore bases. (<b>K</b>,<b>L</b>) Sorus (SEM). (<b>M</b>–<b>O</b>) Sorophores (SEM). (<b>P</b>) Sorophore base (SEM). The red arrows refer to the key distinguishing characteristics.</p>
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<p>Morphological features of <span class="html-italic">Dictyostelium robusticaule</span> Y. Li, P. Liu, Y. Zou (HMJAU B341, B321). (<b>A</b>,<b>B</b>) Sorocarps. (<b>C</b>) Aggregations. (<b>D</b>,<b>E</b>) Pseudoplasmodia. (<b>F</b>) Spores. (<b>G</b>) Sorophore. (<b>H</b>,<b>I</b>) Sorophore tips. (<b>J</b>) Sorophore base. The red arrows refer to the key distinguishing characteristics.</p>
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<p>Morphological features of <span class="html-italic">Dictyostelium purpureum</span> Olive (HMJAU C211). (<b>A</b>) Sorocarps. (<b>B</b>) Aggregations. (<b>C</b>) Pseudoplasmodia. (<b>D</b>) Sorophore. (<b>E</b>) Spores. (<b>F</b>) Sorophore tip. (<b>G</b>) Sorophore base. The red arrows refer to the key distinguishing characteristics.</p>
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<p>Morphological features of <span class="html-italic">Heterostelium pallidum</span> (Olive) S. Baldauf, S. Sheikh, and Thulin (HMJAU C231; C311-C315; C321-C324; C331-C336; C341-C347; C351-C357). (<b>A</b>) Sorocarps. (<b>B</b>) Aggregations. (<b>C</b>) Clustered sorogens. (<b>D</b>) Pseudoplasmodia. (<b>E</b>) Spores. (<b>F</b>) Sorophores. (<b>G</b>) Sorophore tips. (<b>H</b>) Sorophore base. The red arrows refer to the key distinguishing characteristics.</p>
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<p>Phylogeny of all known dictyostelids based on SSU rDNA (<b>A</b>) and closely related species of dictyostelids based on ITS rDNA, newly generated sequences are indicated in bold (<b>B</b>).</p>
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<p>SSU phylogeny of <span class="html-italic">Dictyostelium</span> (<b>A</b>) and <span class="html-italic">Polysphondylium</span> (<b>B</b>) sequences in the order Dictyosteliales and the family Dictyosteliaceae. Numbers in parentheses are SH-aLRT support (%)/ultrafast bootstrap support (%). Newly generated sequences are indicated in bold.</p>
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<p>SSU phylogeny of <span class="html-italic">Heterostelium</span> sequences in the order Acytosteliales, family Acytosteliaceae. Numbers in parentheses are SH-aLRT support (%)/ultrafast bootstrap support (%). Newly generated sequences are indicated in bold.</p>
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<p>The edaphic, biotic, spatial, and climatic factors explaining the diversity of dictyostelid communities with the variables analyzed by RDA. Groups are as follows: Fanjing Mountain Nature Reserve Plot A, Plot B, and Plot C. The significance of variables was tested using ANOVA. The variation partitioning analysis was computed using the significant variables identified within each category. Significance levels are * <span class="html-italic">p</span> &lt; 0.05.</p>
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<p>Analysis of dictyostelid diversity comparisons and geographical characteristics in different protected areas. (<b>A</b>) Map of the protected area. (<b>B</b>) Jaccard similarity coefficients (S<sub>J</sub> %) of dictyostelids in family, genus, and species within Changbai Mountain in Jilin Province (CB), Gushan Mountain in Fujian Province (GS), Baiyun Mountain in Henan Province (BY), Qinghai–Tibet Plateau in Tibet (QT), and Fanjing Mountain in Guizhou Province (FJ). (<b>C</b>,<b>D</b>) Beta diversity was analyzed using PCA; bar chart clustered by Bray–Curtis similarities calculated based on the dictyostelid species of the three climate zones.</p>
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11 pages, 1102 KiB  
Article
Isolation and Structure Determination of New Pyrones from Dictyostelium spp. Cellular Slime Molds Coincubated with Pseudomonas spp.
by Takehiro Nishimura, Takuya Murotani, Hitomi Sasaki, Yoshinori Uekusa, Hiromi Eguchi, Hirotaka Ishigaki, Katsunori Takahashi, Yuzuru Kubohara and Haruhisa Kikuchi
Molecules 2024, 29(9), 2143; https://doi.org/10.3390/molecules29092143 - 5 May 2024
Viewed by 1206
Abstract
Cellular slime molds are excellent model organisms in the field of cell and developmental biology because of their simple developmental patterns. During our studies on the identification of bioactive molecules from secondary metabolites of cellular slime molds toward the development of novel pharmaceuticals, [...] Read more.
Cellular slime molds are excellent model organisms in the field of cell and developmental biology because of their simple developmental patterns. During our studies on the identification of bioactive molecules from secondary metabolites of cellular slime molds toward the development of novel pharmaceuticals, we revealed the structural diversity of secondary metabolites. Cellular slime molds grow by feeding on bacteria, such as Klebsiella aerogenes and Escherichia coli, without using medium components. Although changing the feeding bacteria is expected to affect dramatically the secondary metabolite production, the effect of the feeding bacteria on the production of secondary metabolites is not known. Herein, we report the isolation and structure elucidation of clavapyrone (1) from Dictyostelium clavatum, intermedipyrone (2) from D. magnum, and magnumiol (3) from D. intermedium. These compounds are not obtained from usual cultural conditions with Klebsiella aerogenes but obtained from coincubated conditions with Pseudomonas spp. The results demonstrate the diversity of the secondary metabolites of cellular slime molds and suggest that widening the range of feeding bacteria for cellular slime molds would increase their application potential in drug discovery. Full article
(This article belongs to the Special Issue Discovery of Bioactive Ingredients from Natural Products, 5th Edition)
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Graphical abstract

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<p>Structures of clavapyrone (<b>1</b>), intermedipyrone (<b>2</b>), and magnumiol (<b>3</b>).</p>
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<p>Structure of <b>1</b> and representative <sup>1</sup>H–<sup>1</sup>H COSY and HMBC correlations.</p>
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<p>Structure of <b>2</b> and representative <sup>1</sup>H–<sup>1</sup>H COSY and HMBC correlations.</p>
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<p>Structure of <b>3</b> and representative <sup>1</sup>H–<sup>1</sup>H COSY and HMBC correlations.</p>
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<p>Antiproliferative activity of <b>1</b> against K562 cells.</p>
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25 pages, 62083 KiB  
Article
Optimizing Built Environment in Urban Negative Spaces Using Parametric Methods—Research on a High-Density City in China
by Wenqi Bai, Yudi Wu, Yiwei He, Li Wang, Zining Qiu and Yuqi Ye
Buildings 2024, 14(4), 1081; https://doi.org/10.3390/buildings14041081 - 12 Apr 2024
Cited by 2 | Viewed by 1675
Abstract
In the early stage of architectural design, addressing the challenges posed by negative spaces in high-density urban environments is crucial for enhancing spatial efficiency and building sustainability. Multiple studies employed digital methods and tools to address these issues, such as parametric design, simulation, [...] Read more.
In the early stage of architectural design, addressing the challenges posed by negative spaces in high-density urban environments is crucial for enhancing spatial efficiency and building sustainability. Multiple studies employed digital methods and tools to address these issues, such as parametric design, simulation, and genetic algorithms, to investigate architectural generation approaches for urban negative spaces. This article proposes an integrated design process that involves finding the location and form of negative spaces, generating solutions using slime mold and wasp algorithms, and optimizing and analyzing solutions using the Wallacei plugin in Grasshopper. This comprehensive approach underscores the potential of parametric design to yield a multitude of solutions while also acknowledging the convergence challenges encountered during simulations, particularly in optimizing for optimal sunlight exposure during the winter solstice and minimal solar radiation in the summer. Analyzing the optimization goals and parameter values of the 15th Pareto optimal solution in the 100th generation reveals: (1) a higher number of units leads to positive correlation growth in both objectives; (2) within a certain number of units, parametrically generated solutions facilitate the convergence of optimization goals, yielding optimal outcomes. Therefore, factors such as the range of unit quantities and proportions need consideration during early-stage parametric design and simulation. This study explores a design methodology for negative spaces in high-density urban cities, validating the feasibility of various mainstream generation methods and offering insights for future research. Full article
(This article belongs to the Special Issue Architectural Design Supported by Information Technology: 2nd Edition)
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<p>The relationship between research on negative space and parametric design.</p>
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<p>Methodological approach.</p>
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<p>Diagrams illustrate the content of step 3. (<b>a</b>) Optimal path generated by the slime mould algorithm; (<b>b</b>) aggregated units generated by the Wasp algorithm; (<b>c</b>) volume centroid of each unit; (<b>d</b>) vertical shortest distance from the volume centroid of each unit to the optimal path (D).</p>
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<p>Research site information; (<b>a</b>) the satellite map of the Tianhe district; (<b>b</b>) selected site location; (<b>c</b>) the related parameters of the research site; (<b>d</b>) the 3D model of the research site.</p>
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<p>Research site information; (<b>a</b>) the satellite map of the Tianhe district; (<b>b</b>) selected site location; (<b>c</b>) the related parameters of the research site; (<b>d</b>) the 3D model of the research site.</p>
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<p>Connection rules between units. (<b>a</b>) Constraint points for connection; (<b>b</b>) Constraint lines for connection.</p>
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<p>The multi-objective optimization process.</p>
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<p>Unit quantity (N) statistics plot.</p>
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<p>Spatial distribution plot of all solutions during optimization.</p>
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<p>D value statistics plot.</p>
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<p>Statical analysis. (<b>a</b>) The relation between Pareto solutions and statics of WSD and SSR; (<b>b</b>) The relationship between Pareto solutions and statics of WSD and N value.</p>
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