Search Results (1,583)

The present study investigated the dynamics changes in physicochemical properties and non-volatile metabolites during Bulang pickled tea fermentation. A combination of artificial sensory evaluation, chemical-physical analysis, ultra performance liquid chromatography coupled with quadrupole/time-of-flight mass spectrometry (UPLC-QTOF-MS), and multivariate statistical analysis were employed to examine the differences among four fermentation stages of Bulang pickled tea. The bitterness, astringency, sweetness after taste, sourness and fermentation taste tended to increase with fermentation. The highest lactic acid bacteria, aerobic bacteria, total titratable acidity, total soluble sugar, total polyphenols, and total flavonoids were recorded at the second month of fermentation, while fungi, total free amino acids, total antioxidant capacity and hydroxyl free radical scavenging capacity increased with fermentation. Mantel test demonstrated significant associations between lactic acid bacteria /fungal communities and taste characteristics. UPLC-QTOF-MS analysis led to the identification of 35 differential non-volatile metabolites, predominantly comprising heterocyclic compounds, organic acids with their derivatives, and flavonoids. Nine non-volatile metabolites are related to antioxidant activity, and morin, malvidin and 7-methylxanthine exhibit relatively strong antioxidant activity. This study provides comprehensive insights into the non-volatile metabolites and antioxidant function of Bulang pickled tea. Full article

It is estimated that approximately 25% of waste remains after the apple juice pressing process. Combining this waste biomass with valuable compounds creates the potential for reuse. To create a cost-efficient ecological process without any expensive steps, the aim of this research was to examine the potential of using non-Saccharomyces yeasts (Kazachstania barnettii D1, Hanseniaspora uvarum D9, and Wickerhamomyces anomalus D11) for the low-temperature valorisation of apple pomace. The scope encompassed characteristics of apple pomace and the evaluation of yeast growth and metabolic activity, including carbohydrate consumption, enzymatic activity, and the biosynthesis of volatile organic compounds. Moreover, the effect of inoculum size on biomass increases and the productivity of metabolites during the fermentation of apple pomace were evaluated. To investigate the potential intensification of the process, the experiment was performed on hydrolysed and untreated apple pomace. The obtained results indicate that yeast growth was satisfactory regardless of the inoculum size in both fermentation media. Various activities of peptidases, esterases, phosphatases, and glucosidases were observed. The yeast isolates presented metabolic activity during the process which was confirmed by the production of ethanol and acetic acid. Moreover, a significant amount of volatile organic compounds, especially esters, were synthesised, which have a positive impact on the sensory profile of fermented apple pomace. In general, the hydrolysis of apple pomace did not result in better yeast activity and the formation of aroma compounds. Full article

Kiwifruit is widely recognized for its rich nutritional composition and potential health benefits, yet comparative studies on different cultivars remain limited. In this study, we investigated the physicochemical properties, free sugar and organic acid content, and bioactive compounds in four kiwifruit cultivars: Hayward (HW), Halla Gold (HG), Jecy Gold (JG), and Sweet Gold (SG). This study aimed to determine variations in the composition of these cultivars and assess their antioxidant potential. The pH did not significantly differ among the kiwifruit cultivars. Sweetness and acidity are key sensory attributes in fruit, and SG exhibited the highest acidity, soluble solid content, and reducing sugar content. Accordingly, SG had the highest free sugar (11.25 g/100 mL) and organic acid (13.08 g/100 mL) levels. Phenolic acid (473.01 μg/mL) and flavonol (96.43 μg/mL) contents were most abundant in SG. In this cultivar, chlorogenic acid and epigallocatechin levels were the highest, while epicatechin and naringenin were detected only in SG. Finally, antioxidant activities (i.e., DPPH, ABTS, and hydroxyl radical scavenging activities and FRAP) were highest in SG, followed by HG, JG, and HW. The SG cultivar used in this study exhibits strong antioxidant activity, disease-suppressing effects, skin protection properties, and the potential to reduce the risk of chronic diseases due to its high phenolic compound content. These findings suggest that SG, which possesses excellent taste and functional properties, may serve as a promising candidate for the development of high-quality kiwifruit-based products. Full article

Background and Objective: The global population is struggling with significant health challenges, among which overweight and obesity stand out. Currently, 61% of adults and 7.5% of children and adolescents are affected, underscoring the urgent need for effective solutions. This study evaluated appetite-reducing prototypes related with food products, focusing on their ability to influence appetite through the sense of smell. The objective was to determine the effectiveness of these prototypes and identify the most promising candidates for further research. Methods: A questionnaire-based consumer survey was performed for six appetite-reducing agents. Forty-five participants with elevated body mass index values (BMI ≥ 25) were asked to verify the samples in terms of aroma intensity, pleasure, and potential for appetite reduction. Also, qualitative parameters such as the identification of the samples’ food associations was performed within the questionnaire. The questionnaire results were further compared with headspace solid-phase microextraction (HS-SPME Arrow) analysis results to identify volatile organic compounds associated with appetite-reducing properties. Results: The proof-of-concept study revealed that prototypes with unpleasant and irritating aromas demonstrated the highest appetite-reducing potential, scoring approximately 24 out of 35 points. Conversely, prototypes with pleasant, dessert-like aromas showed lower effectiveness, scoring between 14 and 18 points. Conclusions: By linking consumer perceptions to chemical analyses, we identified effective prototypes for further investigation, including studies measuring actual food intake. These findings contribute to developing innovative, non-invasive strategies to address overweight and obesity, offering a new dimension to appetite control through sensory modulation. Full article

Background: Autism Spectrum Disorder (ASD) is a neurodevelopmental disorder characterized by persistent difficulties in social interaction, communication, and repetitive behaviors. Neuroimaging studies have revealed structural and functional neural changes in individuals with ASD compared to healthy subjects. Objectives: This study aimed to investigate brain network structural connectivity in ASD using Morphometric Similarity Network (MSN) analysis. Methods: Data from the Autism Brain Imaging Data Exchange (ABIDE) were analyzed, comprising 597 individuals with ASD and 644 healthy controls. Structural connectivity was assessed using cortical morphometric features. Global and regional network indices, including the density index, node degree, node strength, and clustering coefficients, were evaluated. Results: Among the global network indices, when using a threshold value of 0.4, ASD patients compared to HCs showed a lower density (p = 0.041) and higher negative clustering (p = 0.0051) coefficients. For regional network indices, ASD patients showed a lower bilateral superior frontal cortices degree (left hemisphere: p = 0.014; right hemisphere: p = 0.0038) and strength (left: p = 0.017; right: p = 0.018). Additionally, they showed higher negative clustering coefficients in the bilateral superior frontal cortices (left, p = 0.0088; right, p = 0.0056) and bilateral pars orbitalis (left, p = 0.016; right, p = 0.0006), as well as lower positive clustering in the bilateral frontal pole (left, p = 0.03; right, p = 0.044). Conclusions: These findings highlight significant alterations in both global and regional brain network organization in ASD, which may contribute to the disorder’s cognitive and behavioral manifestations. Future studies are needed to investigate the pathophysiological mechanisms underlying these structural connectivity changes, to inform the development of more targeted and individualized therapeutic interventions for individuals with ASD. Full article

The flavour of foxtail millet (Setaria italica (L.) P. Beauv.) is an important indicator for evaluating the quality of the millet. The volatile components in steamed millet porridge samples were analysed using electronic nose (E-Nose) and gas chromatography–ion mobility spectrometry (GC-IMS) techniques, and characteristic volatile fingerprints were constructed to clarify the differences in the main flavour substances in different foxtail millet varieties (two hybrids and two conventional foxtail millets). After sensory evaluation by judges, Jingu 21 (JG) scored significantly higher than the other varieties, and the others were, in order, Jinmiao K1 (JM), Changzagu 466 (CZ) and Zhangzagu 3 (ZZ). E-Nose analysis showed differences in sulphides and terpenoids, nitrogen oxides, organosulphides and aromatic compounds in different varieties of millet porridge. A total of 59 volatile components were determined by GC-IMS in the four varieties of millet porridge, including 23 aldehydes, 17 alcohols, 9 ketones, 4 esters, 2 acids, 3 furans and 1 pyrazine. Comparative analyses of the volatile components in JG, JM, ZZ and CZ revealed that the contents of octanal, nonanal and 3-methyl-2-butenal were higher in JG; the contents of trans-2-butenal, 2-methyl-1-propanol, trans-2-heptenal and trans-2-pentenal were higher in JM; and the contents of 2-octanone, hexanol, 1-octen-3-ol, 2-pentanone and butyraldehyde were higher in ZZ. The contents of 2-butanol, propionic acid and acetic acid were higher in CZ. A prediction model with good stability was established by orthogonal partial least squares discriminant analysis (OPLS-DA), and 25 potential characteristic markers (VIP > 1) were screened out from 59 volatile organic compounds (VOCs). These volatile components can be used to distinguish the different varieties of millet porridge samples. Moreover, we found conventional foxtail millet contained more aldehydes than the hybridised foxtail millet; especially decanal, 1-nonanal-D, heptanal-D, 1-octanal-M, 1-octanal-D and 1-nonanal-M were significantly higher in JG than in the other varieties. These results indicate that the E-Nose combined with GC-IMS can be used to characterise the flavour volatiles of different foxtail millet, and the results of this study may provide some information for future understanding of the aroma characteristics of foxtail millet and the genetic improvement of hybrid grains. Full article

The growing global focus on the adverse health conditions associated with excessive sugar consumption has prompted health and policy organizations as well as the public to take a more mindful approach to health and wellness. In response, food and beverage companies have proactively innovated and reformulated their product portfolios to incorporate low and no-calorie sweeteners (LNCSs) as viable alternatives to sugar. LNCSs offer an effective and safe approach to delivering sweetness to foods and beverages and reducing calories and sugar intake while contributing to the enjoyment of eating. The objective of this paper is to enhance the understanding of LNCSs segmentation and definitions, dietary consumption and reduction guidance, front-of-package labeling, taste and sensory perception and physiology, metabolic efficacy and impact, as well as the overall safety of LNCSs and sugar. Full article

Endometriosis is a chronic, inflammatory condition where endometrial-like tissue grows outside the uterus, affecting around 10% of women of reproductive age. This condition is associated with debilitating symptoms, including dysmenorrhea, dyspareunia, chronic pelvic pain, fatigue, and infertility. Adolescents with endometriosis face unique challenges, as the disease is often misdiagnosed or undiagnosed for an average of 7–10 years due to its complex and multifactorial nature. Consequently, patients frequently suffer from worsening symptoms and significant psychological distress, including anxiety, depression, and social withdrawal. While there is no definitive cure for endometriosis, treatment approaches typically involve hormonal therapies, lifestyle adjustments (such as diet and exercise), and psychological support. Recent studies emphasize the profound impact of endometriosis on the mental health of adolescents, highlighting the need for a more holistic treatment approach that integrates both medical and psychological care. This narrative review explores the psychological and psychosocial effects of endometriosis in adolescents, examining the biological and psychological mechanisms linking the disease to mental health outcomes. It also discusses current therapeutic strategies, such as cognitive behavioral therapy, mindfulness, and peer support, and underscores the importance of early diagnosis and multidisciplinary care to mitigate both the physical and emotional burdens of the condition. This integrated approach is critical in improving the overall well-being and quality of life for adolescents living with endometriosis. Full article

Early diagnosis of subclinical ketosis is fundamental in the production management of dairy cattle. Without evident clinical signs, this pathological condition causes important economic losses for the farmer and significant health repercussions for the cattle that could develop an altered immune function. Laboratory techniques, although accurate, are expensive, invasive, and cannot be used for real-time monitoring of the entire herd. On the contrary, the analysis of volatile organic compounds (VOCs) contained in the breath of dairy cattle affected by ketosis could represent a key biomarker of the ketogenic process. For this reason, we developed a sensory device, tested in the laboratory, to detect acetone concentrations ranging from 1 to 10 ppm (concentrations typically detected in the cow’s breath), and we look to verify the electronic nose’s potential as a non-invasive diagnostic tool for ketosis. Experimental results show the high sensitivity of the instrument in differentiating acetone solutions. Principal component analysis (PCA) showed a clear separation of samples in the score plot, while classification using linear discriminant analysis (LDA) and quadratic discriminant analysis (QDA) achieved accuracy rates above 70% and 85%, respectively. These findings suggest the potential application of the electronic nose as a non-invasive diagnostic tool in veterinary diagnostic studies. In particular, its ability to detect and discriminate low acetone concentrations could help the farmer to improve the overall management of the herd, optimising monitoring strategies and ketosis diagnosis before the appearance of the clinical signs of the disease. Full article

Introduction: In patients refractory to vestibular rehabilitation in the management of bilateral vestibulopathy, the cochleo-vestibular implant has emerged as a viable alternative to enhance both audiovestibular function and quality of life. The main objective of this study is to pioneer the use of PET to assess cortical modifications in patients with cochleo-vestibular implants, aiming to evaluate the safety and functional improvements in individuals with bilateral vestibulopathy and severe to profound hearing loss. Methods: A phase I pilot clinical trial was conducted with participants who received a BIONIC-VEST CI24RE cochleo-vestibular implant, with pre- and post-implantation assessments conducted for twelve months. Audiovestibular testing and two PET studies with 18F-FDG under baseline conditions and with active stimulus to observe cortical-level differences were performed. Results: Five patients were included in the study, all of them treated with a cochleo-vestibular implant, none of whom presented postoperative adverse effects. Audiologically, the mean post-implant gain was 56.63 ± 14.53 dB and 50.40 ± 35.54% in terms of speech intelligibility. From a vestibular perspective, the most remarkable findings were observed at the graviceptive pathway level, where a mean posturographic improvement was observed, with a sensory organization test score of 24.20 ± 13.74 and a subjective visual vertical of 1.57° ± 0.79°, achieving, in most cases, results within the normal range (<2.3°) by the end of the follow-up. PET images confirmed that with the electrical stimulus active (implant ON), there was a supratentorial activation pattern, particularly in areas related to somatosensory integration, emotional regulation, and autonomic control. Conclusions: The BIONIC-VEST implant significantly improved the vestibular system, particularly the graviceptive pathway, enhancing balance and SVV and reducing fall risk. PET revealed distinct uptake patterns in baseline and activated conditions, highlighting a cortical-level response with the use of the cochleo-vestibular implant. Full article

Two different diets able to induce dietary hyperammonaemia (a methionine–choline-deficient (MCD) diet and a methionine-deficient diet enriched with ammonium acetate (MAD + 20% ammonium acetate)) were tested in a rat model. The diets were shown to have different modes of action, inducing significant hyperammonaemia (HA) and growth retardation in the rats, with different metabolic consequences. The MCD diet resulted in the development of endogenous HA, with a decrease in bilirubin levels and an increase in hepatic fat content. In contrast, the MAD + 20% ammonium acetate diet increased circulating ALP and haptoglobin levels and decreased liver mass. The above results suggest that the MCD diet deteriorated the liver function of the rats, resulting in the development of endogenous HA, while the MAD diet caused moderate changes in liver metabolism, resulting in the development of exogenous HA. Interestingly, the commonly used oral treatments Lactulose and Rifaximin did not ameliorate hyperammonaemia during or after the treatment period. In conclusion, even though the diets used in the current study caused somewhat similar hyperammonaemia, they seemed to provoke different metabolic consequences. The latter can have an impact on the severity of the resulting hyperammonaemia and thus on the hyperammonaemia-induced encephalopathy, resulting in the development of distinguishing cognitive and metabolic (liver) effects compared to other forms of encephalopathy. We hypothesized that these rat models, with significantly increased serum ammonia levels, along with different liver injuries, could serve as a suitable double animal model for the testing of new, oral enzyme therapies for hepatic encephalopathy in future studies. Full article

Cocoa quality serves as a differentiating factor that provides monetary and non-monetary benefits to farmers, defined by the genotype, agroecological conditions of cultivation, and the post-harvest processes involved in transforming seeds into cocoa beans, including harvesting, pre-conditioning, fermentation, and drying. Drying plays a crucial role in ensuring the sensory, chemical, and microbiological quality of the beans, as simultaneous mass and heat transfer phenomena occur during this process, along with chemical reactions (both enzymatic and non-enzymatic) that influence the concentration and dynamics of phenolic compounds, organic acids, methylxanthines, and the formation of volatiles, directly impacting flavor development in cocoa beans. This paper comprehensively reviews cocoa drying methods, variables, and equipment and analyzes their impact on these flavor-determining compounds. The findings highlight that drying significantly contributes to the production of differentiated and specialty quality traits. An integral relationship between the methods, operating variables, and drying equipment applied to cocoa and their implications for the volatile and non-volatile compounds is described. Full article

This study aimed to investigate the flavor formation and meat quality differences among different beef cuts in Chinese Wagyu cattle. The metabolites and gene expression profiles of chuck, neck, rump, tenderloin, and longissimus lumborum cuts were analyzed. The results revealed that a total of 240 volatile organic compounds and 779 lipid molecules were detected among the beef cuts, with hydrocarbons (accounting for 29.71%) and triglycerides (representing 41.21%) emerging as the most prominent compounds, respectively. The sensory-directed analysis highlighted the significance of sweet and fruity aroma compounds, which contributed to the distinct aroma profiles among different beef cuts. Additionally, a total of 60 key lipid molecular markers, including FA(18:1), PC(40:5), TG(18:0_16:1_18:1), and TG(36:0_18:1), etc., were identified as playing crucial roles in the generation of essential lipid compounds across five different beef cuts. Integrative analysis of multi-omics data pinpointed a cluster of differentially expressed genes (e.g., DLD, ACADM, PCCA, SCD), which were involved in the regulation of valine, leucine, and isoleucine degradation pathways and lipid metabolism. Taken together, this study has identified key metabolites and candidate genes influencing meat quality across different beef cuts, providing a valuable resource for the molecular breeding of high-quality traits in beef cattle. Full article

As an important crop in the world, dried pepper is widely used in various foods. However, the sensory quality, fruit shape index, edible index, nutrition index, and volatile components of dried pepper have not been comprehensively analyzed. This study elucidated the differences between different varieties of dried pepper and provided the basis for the selection of raw materials for different varieties of dried pepper products. The varieties with high scores in sensory evaluation were Henan new generation, Neihuang new generation, Chengdu Erjingtiao, India S17, and Honglong 12. The varieties with the highest fruit shape index, edible rate, and nutrition index were Chengdu Erjingtiao and Guizhou Erjingtiao. A total of 380 volatile organic compounds were identified by comprehensive two-dimensional gas chromatography–time-of-flight mass spectrometry with headspace sampling (HS-GC-TOF MS), including 62 alcohols, 50 aldehydes, 68 ketones, 60 hydrocarbons, 99 esters, 18 acids, and 23 other substances such as pyrazoles and ethers. Full article

Postural abnormalities in the sagittal plane are common in Parkinson’s disease (PD) and increase the risk of falls. Previous studies have reported short-term benefit of corrective corsets in PD patients assessed by clinical and instrumental methods, while long-term effects on sagittal posture in upright standing and during walking remain unexplored. Fifteen PD patients with postural abnormalities on the sagittal plane, evaluated via the NeuroPostureApp, and ten healthy subjects matched for age and BMI were assessed by 3D motion analysis in upright posture. Then, the PD patients were evaluated with and without the K1 Posture Keeper during standing and walking at baseline (T0) and after three months of use (T1). The results showed an anteriorization of the head–cervical region with respect to the trunk and a whole-body misalignment in PD patients compared to healthy controls. The use of the K1 Posture Keeper induced a back shift of the nasion with a better alignment of the head with respect to the trunk, pelvis, and feet in upright standing and during walking, underlining an improvement in the sagittal alignment of the entire body in PD. These findings showed the therapeutical role of K1 Posture Keeper on sagittal posture in static and dynamic conditions, potentially due to proprioceptive reorganization. Full article