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17 pages, 830 KiB  
Systematic Review
Laser Applications in Metal Orthodontic Bracket Debonding: A Systematic Review
by Patryk Woś, Sylwia Kiryk, Tomasz Dyl, Jan Kiryk, Tomasz Horodniczy, Magdalena Szablińska, Magdalena Aleksandra Dubowik, Wojciech Dobrzyński, Marcin Mikulewicz, Jacek Matys and Maciej Dobrzyński
Appl. Sci. 2025, 15(2), 927; https://doi.org/10.3390/app15020927 (registering DOI) - 18 Jan 2025
Viewed by 135
Abstract
Objective: The aim of this systematic review was to evaluate the effectiveness and safety of various laser wavelengths for debonding orthodontic metal brackets compared to traditional plier-based methods. The primary outcomes assessed were enamel damage, pulp temperature changes, adhesive remnant index (ARI), and [...] Read more.
Objective: The aim of this systematic review was to evaluate the effectiveness and safety of various laser wavelengths for debonding orthodontic metal brackets compared to traditional plier-based methods. The primary outcomes assessed were enamel damage, pulp temperature changes, adhesive remnant index (ARI), and shear bond strength (SBS). Materials and Methods: In September 2024, an electronic search was performed across the PubMed, Web of Science (WoS), and Scopus databases, adhering to the PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-Analyses) guidelines and the PICO framework. The initial search yielded 453 records. After eliminating 256 duplicates, 197 unique records were left for screening, which ultimately led to the qualification of 8 articles that met the inclusion criteria for both qualitative and quantitative analyses. The risk of bias in the articles was assessed by two independent reviewers. Results: The included studies demonstrated that laser-assisted debonding generally resulted in less adhesive residue on the enamel surface compared to conventional methods, as evidenced by the reductions in ARI scores reported in two studies. Temperature increases during laser use varied depending on the laser type and power settings. The Nd:YAG (neodymium-yttrium, aluminum, garnet) laser was found to cause significant temperature rises, posing a potential risk to pulp tissue, while the Er:YAG (erbium—yttrium, aluminum, garnet) and Er,Cr:YSGG (erbium, chromium—yttrium, scandium, gallium, garnet) lasers produced only negligible increases in pulp temperature. SBS comparisons revealed no significant differences between the laser-assisted and traditional debonding methods. Additionally, diode lasers demonstrated the potential to minimize enamel damage, particularly when operated at lower power settings. Four publications were assessed as high quality (low risk of bias), and another four as moderate quality (average risk of bias). Conclusions: In conclusion, laser-assisted orthodontic metal bracket debonding, when conducted with appropriately calibrated parameters, is a safe method for preserving tooth tissue. However, its advantages appear to be minimal compared to conventional plier-based methods, highlighting the need for further research to justify its broader clinical application. Full article
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<p>Concepts describing the mechanism of debonding using lasers.</p>
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<p>The PRISMA protocol.</p>
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19 pages, 7054 KiB  
Article
Effect of Gradient Transition Layer on the Cracking Behavior of Ni60B (NiCrBSi) Coatings by Laser Cladding
by Qi Sun, Weiming Bi, Shan Yao, Wenxu Zhu, Wenjian Ma, Bing Hu, Cuimin Bao, Yong Zhang and Fangyong Niu
Materials 2025, 18(2), 419; https://doi.org/10.3390/ma18020419 - 17 Jan 2025
Viewed by 180
Abstract
Laser cladding technology is an effective method for producing wear-resistant coatings on damaged substrates, improving both wear and corrosion resistance, which extends the service life of components. However, the fabrication of hard and brittle materials is highly susceptible to the problem of cracking. [...] Read more.
Laser cladding technology is an effective method for producing wear-resistant coatings on damaged substrates, improving both wear and corrosion resistance, which extends the service life of components. However, the fabrication of hard and brittle materials is highly susceptible to the problem of cracking. Using gradient transition layers is an effective strategy to mitigate the challenge of achieving crack-free laser-melted wear-resistant coatings. This study presents the cracking issue of laser cladding Ni60B (NiCrBSi) coatings on 38CrMoAl (18CrNiMo7-6) steel by designing a gradient transition layer infused with varying amounts of Ni powder. We examine how different levels of Ni doping in the transition layer influence the fabrication of the Ni60B coating. The results indicate that the cracking mechanism of Ni60B is primarily due to the brittleness and hardness of the fusion cladding layer, which can result in cold cracks under residual tensile stress. Increasing the nickel content in the transition layer reduces the difference in thermal expansion coefficients between the cladding layer and the substrate. Additionally, the nickel in the transition layer permeates the cladding layer due to the laser remelting effect. The physical phase within the cladding layer transitions from the initial CrB, M7C3, and γ-Ni solid solution to γ-Ni solid solution and Ni-B-Si eutectic, with a small amount of boride and carbide hard phases. As the nickel doping in the transition layer increases, the proportion of the toughness phase dominated by Ni elements significantly rises, leading to a decrease in the hardness of the fused cladding layer. However, the average hardness of the fusion cladding layer in crack-free samples was measured at 397.5 ± 5.7 HV0.2, which is 91% higher than that of the substrate. Full article
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<p>Schematic illustrations of laser cladding forming system.</p>
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<p>Experimental material: (<b>a</b>) Ni60B powder, (<b>b</b>) Ni powder, (<b>c</b>) 38CrMoAl substrate.</p>
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<p>Experimental schematic.</p>
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<p>Penetrant inspection results of coating.</p>
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<p>Number of cracks statistics.</p>
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<p>Single-layer multi-beads coatings and penetrant inspection result: (<b>a</b>,<b>d</b>) P = 1600, V = 2 mm/s, and H = 0.6 mm. (<b>b</b>,<b>e</b>) P = 1600 W, V = 6 mm/s, and H = 0.2 mm. (<b>c</b>,<b>f</b>) P = 1600, V = 6 mm/s, and H = 0.6 mm.</p>
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<p>Fracture morphology and micro-crack morphology diagram: (<b>a</b>) macroscopic fracture morphology, (<b>b</b>) high-magnification fracture morphology, and (<b>c</b>) micro-crack morphology.</p>
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<p>XRD of Ni60B coatings.</p>
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<p>Phase and EDS analysis of Ni60B coating: (<b>a</b>) macro phases, (<b>b</b>) Microstructure of the upper part of the coating, (<b>c</b>) Microstructure of the middle part of the coating, (<b>d</b>) Microstructure of the upper part of the coating, (<b>e</b>) EDS analysis of the middle part of the coating, (<b>f</b>) EDS analysis of the middle part of the coating, (<b>g</b>) EDS analysis of the bottom part of the coating.</p>
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<p>Phase of Ni60B coating: (<b>a</b>) upper part of coating, (<b>b</b>) middle part of coating, and (<b>c</b>) bottom part of coating.</p>
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<p>Penetration inspection results of different transition schemes (Red lines are cracks showing colour): (<b>a</b>) transition scheme a. (<b>b</b>) Transition scheme b. (<b>c</b>) Transition plan c. (<b>d</b>) Transition plan d. (<b>e</b>) Transition plan e.</p>
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<p>XRD patterns of different Ni doping qualities.</p>
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<p>Physical phase and EDS analysis of cladding layer with different transition schemes: (<b>a</b>) Microstructure of scheme a, (<b>b</b>) Microstructure of scheme b, (<b>c</b>) Microstructure of scheme c, (<b>d</b>) Microstructure of scheme d, and (<b>e</b>) Microstructure of scheme e, (<b>f</b>) Analysis of scheme a, (<b>g</b>) Analysis of scheme b, (<b>h</b>) Analysis of scheme c, (<b>i</b>) Analysis of scheme d, (<b>j</b>) Analysis of scheme e.</p>
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<p>Physical phase and EDS analysis of transition layer with different transition schemes: (<b>a</b>) Microstructure of scheme a, (<b>b</b>) Microstructure of scheme b, (<b>c</b>) Microstructure of scheme c, (<b>d</b>) Microstructure of scheme d, and (<b>e</b>) Microstructure of scheme e, (<b>f</b>) Analysis of scheme a, (<b>g</b>) Analysis of scheme b, (<b>h</b>) Analysis of scheme c, (<b>i</b>) Analysis of scheme d, (<b>j</b>) Analysis of scheme e.</p>
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<p>Microhardness of different transition schemes.</p>
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10 pages, 3582 KiB  
Article
Clinical Efficacy of Three-Dimensional-Printed Pure Titanium Fracture Plates with Locking Screw Systems in Distal Tibia Fractures
by Ji Hye Choi, Jun Hyoung Lee, Seung Hyeop Lee and Woo Young Jang
Medicina 2025, 61(1), 137; https://doi.org/10.3390/medicina61010137 - 15 Jan 2025
Viewed by 455
Abstract
Background and Objectives: Distal tibia fractures are high-energy injuries characterized by a mismatch between standard plate designs and the patient’s specific anatomical bone structure, which can lead to severe soft tissue damage. Recent advancements have focused on the development of customized metal plates [...] Read more.
Background and Objectives: Distal tibia fractures are high-energy injuries characterized by a mismatch between standard plate designs and the patient’s specific anatomical bone structure, which can lead to severe soft tissue damage. Recent advancements have focused on the development of customized metal plates using three-dimensional (3D) printing technology. However, 3D-printed metal plates using titanium alloys have not incorporated a locking system due to the brittleness of these alloys. Therefore, this study aimed to determine whether a locking mechanism can be effectively implemented using 3D-printed pure titanium and further evaluate the clinical outcomes of such implants in patients with distal tibia fractures. Materials and Methods: Between March 2021 and June 2022, nine patients who underwent open reduction and internal fixation for distal tibia fractures using 3D-printed pure titanium plates were enrolled. Pure titanium powder (Ti Gr.2, Type A, 3D Systems, USA) was spread to a thickness of 30 μm and partially sintered using a 500 W laser to produce the 3D-printed metal plates. The locking screws were fabricated using a milling process. Open reduction and internal fixation were performed on the nine patients using 10 customized plates. The clinical efficacy was analyzed using the union rate, and complications, such as infection and skin irritation, were evaluated to ensure a comprehensive outcome assessment. Results: Surgical treatment was successfully performed on nine patients, with nine of ten plates remaining stable and undamaged. However, one patient with neurofibromatosis experienced a fractured metal plate, which necessitated revision surgery using a metal rod. No screw loosening or surgical wound complications occurred. Conclusions: This study showed that 3D-printed pure titanium plates with integrated locking screw systems provide a viable and effective solution for managing distal tibia fractures. Three-dimensional printing and pure titanium show promise for orthopedic advancements. Full article
(This article belongs to the Section Orthopedics)
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Figure 1
<p>(<b>A</b>) Simulation model of the fractured tibia aligned correctly; (<b>B</b>) Custom fracture plate design upon simulation model; (<b>C</b>) Individual fixation plates designed with positioning screw holes for cortical and locking screws.</p>
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<p>(<b>A</b>) Fracture plate fabricated through metal 3D printing; (<b>B</b>) Jig for locking screw thread; (<b>C</b>) Locking screw hole.</p>
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<p>(<b>A1</b>) Test method according to ASTM F382-17DP; (<b>A2</b>) Fracture plate under actual test according to ASTM F382-17 test standard; (<b>A3</b>) Test results.</p>
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<p>Failed fracture plate case: A 59-year-old female pedestrian was diagnosed with an open tibiofibular shaft fracture and subdural hematoma (SDH) following a traffic accident in which she was hit by a taxi. The patient had a medical history of neurofibromatosis. After massive irrigation and confirmed wound stabilization, minimally invasive percutaneous plating (MIPO) using a 3D-printed plate was performed 4 days after the trauma. The wound healed well without an additional coverage operation. Partial weight bearing was initiated 6 weeks post-operation. Three months post-operation, the patient presented to the outpatient clinic with lower leg pain; a plain radiograph confirmed plate breakage. She subsequently underwent reoperation with plate removal and conversion to an intramedullary (IM) nail fixation. An intraoperative biopsy of the nonunion site revealed findings consistent with chronic inflammation with fibrosis and the presence of dead bone fragments. After tibia IM nail fixation, fracture union was achieved, and the patient’s AOFAS score was 70. R means right sided.</p>
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<p>Customized fracture plate designed to be combined using a tibia intramedullary nail. (<b>A</b>) A 68-year-old male presented to the emergency department after sustaining an injury to his left lower leg with a heavy load. On arrival, the patient was diagnosed with an open tibiofibular shaft fracture. The patient had a medical history of hypertension. An additional fracture line was identified in the distal tibia, raising concerns that adequate stability could not be achieved with an intramedullary nail alone. (<b>B</b>) Therefore, supplementary plating was considered. (<b>C</b>) After massive irrigation and confirmed wound stabilization, closed reduction and internal fixation with a tibia intramedullary (IM) nail and additional 3D-printed plate fixation were performed 1 week after the trauma. The soft tissue healed well without an additional coverage operation. Partial weight bearing was initiated 6 weeks post-operation. A successful union was achieved with an AOFAS score of 70.</p>
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11 pages, 5329 KiB  
Communication
Radiation-Induced Wavelength Shifts in Fiber Bragg Gratings Exposed to Gamma Rays and Neutrons in a Nuclear Reactor
by G. Berkovic, S. Zilberman, Y. London, M. Rosenfeld, E. Shafir, O. Ozeri, K. Ben-Meir, A. Krakovich and T. Makmal
Sensors 2025, 25(2), 323; https://doi.org/10.3390/s25020323 - 8 Jan 2025
Viewed by 340
Abstract
Fiber Bragg gratings (FBGs) inscribed by UV light and different femtosecond laser techniques (phase mask, point-by-point, and plane-by-plane) were exposed—in several irradiation cycles—to accumulated high doses of gamma rays (up to 124 MGy) and neutron fluence (8.7 × 1018/cm2) [...] Read more.
Fiber Bragg gratings (FBGs) inscribed by UV light and different femtosecond laser techniques (phase mask, point-by-point, and plane-by-plane) were exposed—in several irradiation cycles—to accumulated high doses of gamma rays (up to 124 MGy) and neutron fluence (8.7 × 1018/cm2) in a research-grade nuclear reactor. The FBG peak wavelengths were measured continuously in order to monitor radiation-induced shifts. Gratings inscribed on pure silica core fibers using near-IR femtosecond pulses through a phase mask showed the smallest shifts (<30 pm), indicating that these FBGs are suitable for temperature measurement even under extreme ionizing radiation. In contrast, the pointwise inscribed femtosecond gratings and a UV-inscribed grating showed maximal shifts of around 100 pm and 400 pm, respectively. Radiation-induced red shifts are believed to arise from gamma radiation damage, which may partially recover after irradiation is stopped. At the highest neutron exposures, grating peak blue shifts started to appear, apparently due to fiber compaction. Full article
(This article belongs to the Special Issue Optical Fiber Sensors in Radiation Environments: 2nd Edition)
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Figure 1
<p>A visual demonstration of the difference between femtosecond phase mask and femtosecond point-by-point FBGs. (<b>left</b>) The green spool is a connectorized fiber containing point-by-point FBGs, and the smaller blue spool is a fiber with phase mask FBGs. Both fibers are illuminated using red light from fiber fault locators. In a bright room, scattering is visible from the point-by-point FBGs but not from the phase mask FBGs. (<b>right</b>) Weak scattering from the phase mask FBGs can only be observed when the room is completely darkened.</p>
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<p>The reflection spectra of the FBGs used in this study, as measured by an Optical Spectrum Analyzer (OSA). All graphs are presented with the FBG reflected power normalized with respect to its maximum. In all graphs, the full scale along the x-axis is the same (5 nm), enabling an easy comparison of the bandwidths of the different FBGs.</p>
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<p>(<b>a</b>) A photograph of fibers on an aluminum bar and the irradiation tube into which they are placed and lowered into the reactor. The five FBGs and the thermocouple sensing head are all positioned (to within 1 cm) at the same location on the bar to ensure equal exposures during the experiment. (<b>b</b>) Experimental set-up for monitoring FBG peaks and temperature during the experiment.</p>
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<p>Changes in the FBG peak wavelengths (corrected for temperature variations) during and after the first reactor operation shift.</p>
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<p>Accumulated changes in the FBG peak wavelengths (corrected for temperature variations) before, during, and after the second reactor operation shift.</p>
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<p>Changes in the FBG peak wavelengths (corrected for temperature variations) during and after the fifth reactor operation shift (first operation in the stronger irradiation site). The inset at the bottom also shows the partial recovery of the peak shift after shutdown and rapid erasure at the start of the next shift.</p>
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<p>Cumulative shifts in FBG peak wavelengths at the conclusions of all reactor operations expressed as a function of radiation exposure. The bottom horizontal axis is the accumulated dose of γ-rays, and the neutron doses accumulated in parallel are given on the top axis and in the table at bottom.</p>
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15 pages, 9871 KiB  
Article
Study on the Tribological Behavior of Laser Surface Texturing on Silicon Nitride Ceramic Under Water Lubrication
by Hong-Jian Wang, Jing-De Huang, Bo Wang, Yang Zhang and Jin Wang
Lubricants 2025, 13(1), 21; https://doi.org/10.3390/lubricants13010021 - 8 Jan 2025
Viewed by 395
Abstract
The tribological behavior of silicon nitride (Si3N4) ceramic with textured patterns under water lubrication was investigated in this paper. Different textured patterns were fabricated using laser surface texturing (LST). Surface wettability was characterized by contact angle. The original surface [...] Read more.
The tribological behavior of silicon nitride (Si3N4) ceramic with textured patterns under water lubrication was investigated in this paper. Different textured patterns were fabricated using laser surface texturing (LST). Surface wettability was characterized by contact angle. The original surface and textured Si3N4 ceramic with triangular patterns presented as hydrophobic. However, the textured Si3N4 ceramic with hexagonal patterns presented as hydrophilic. Surface wettability and textured patterns were important factors affecting the friction performance of the Si3N4 ceramic. Our results indicated that symmetrical textured patterns were more beneficial for decreasing the coefficient of friction (COF) at lower reciprocating frequencies. In contrast, better surface wettability played a more important role in reducing the COF at higher reciprocating frequencies. The most severe damage observed on the untextured Si3N4 ceramic led to a higher wear rate. The symmetrical structure of hexagonal patterns was more conducive to decreasing the wear rate than triangular patterns. However, the Si3N4 ceramic with triangular patterns was more suitable for use at high-speed frictions due to better lubrication. The textured patterns had the function of storing lubricants and capturing and cutting debris. Thus, friction performance was improved by introducing textured patterns onto the surface of the Si3N4 ceramic. The friction and wear mechanisms are also discussed in this study. Full article
(This article belongs to the Special Issue Anti-wear Lubricating Materials)
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<p>The schematic diagram of different patterns on Si<sub>3</sub>N<sub>4</sub> ceramic surface: (<b>a</b>) triangles and (<b>b</b>) hexagons.</p>
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<p>The optical image (<b>a</b>) and transverse profile (<b>b</b>) of textured patterns after LST.</p>
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<p>The wetting state and CA of the Si<sub>3</sub>N<sub>4</sub> ceramic: (<b>a</b>) UP, (<b>b</b>) TP, and (<b>c</b>) HP.</p>
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<p>COF of the Si<sub>3</sub>N<sub>4</sub> ceramic with different textured patterns as a function of sliding time: (<b>a</b>) 0.5 Hz, (<b>b</b>) 1.5 Hz and (<b>c</b>) 2.5 Hz.</p>
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<p>COF of the Si<sub>3</sub>N<sub>4</sub> ceramic under different reciprocating frequencies.</p>
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<p>SEM of the wear morphology of the Si<sub>3</sub>N<sub>4</sub> ceramic at the reciprocating frequency of 0.5 Hz: (<b>a</b>) UP; (<b>b</b>) TP; (<b>c</b>) HP; (<b>d</b>–<b>f</b>) are enlarged views of UP, TP, and HP, respectively.</p>
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<p>SEM of the wear morphology of the Si<sub>3</sub>N<sub>4</sub> ceramic at the reciprocating frequency of 1.5 Hz: (<b>a</b>) UP; (<b>b</b>) TP; (<b>c</b>) HP; (<b>d</b>–<b>f</b>) are enlarged views of UP, TP, and HP, respectively.</p>
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<p>SEM of the wear morphology of the Si<sub>3</sub>N<sub>4</sub> ceramic at the reciprocating frequency of 2.5 Hz: (<b>a</b>) UP; (<b>b</b>) TP; (<b>c</b>) HP; (<b>d</b>–<b>f</b>) are enlarged views of UP, TP, and HP, respectively.</p>
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<p>EDS analysis from HP at the reciprocating frequency of 2.5 Hz: (<b>a</b>) SEM of debris on the wear track, and (<b>b</b>) EDS at area A and (<b>c</b>) EDS at area B in (<b>a</b>), respectively.</p>
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<p>The wear rate of the WC ball under different reciprocating frequencies.</p>
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<p>Schematic diagram of a water droplet sliding on the Si<sub>3</sub>N<sub>4</sub> ceramic: (<b>a</b>) hydrophobic surface and (<b>b</b>) hydrophilic surface.</p>
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<p>Schematic diagram of the frictional process on the surface of the Si<sub>3</sub>N<sub>4</sub> ceramic: initial stage on the untextured (<b>a</b>) and textured surfaces and (<b>b</b>) frictional stage of UP (<b>c</b>), TP (<b>d</b>), and HP (<b>e</b>).</p>
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11 pages, 4719 KiB  
Article
TBC Development on Ti-6Al-4V for Aerospace Application
by Renata Jesuina Takahashi, João Marcos Kruszynski de Assis, Leonardo Henrique Fazan, Laura Angélica Ardila Rodríguez, Aline Gonçalves Capella and Danieli Aparecida Pereira Reis
Coatings 2025, 15(1), 47; https://doi.org/10.3390/coatings15010047 - 3 Jan 2025
Viewed by 465
Abstract
The Ti-6Al-4V alloy is widely utilized in the aerospace industry for applications such as turbine blades, where it is valued for its mechanical strength at high temperatures, low specific gravity, and resistance to corrosion and oxidation. This alloy provides crucial protection against oxidation [...] Read more.
The Ti-6Al-4V alloy is widely utilized in the aerospace industry for applications such as turbine blades, where it is valued for its mechanical strength at high temperatures, low specific gravity, and resistance to corrosion and oxidation. This alloy provides crucial protection against oxidation and thermal damage. A thermal barrier coating (TBC) typically consists of a metallic substrate, a bond coating (BC), a thermally grown oxide (TGO), and a topcoat ceramic (TC). This study aimed to investigate laser parameters for forming a TBC with a NiCrAlY bond coating and a zirconia ceramic topcoat, which contains 16.0% equimolar yttria and niobia. The coatings were initially deposited in powder form and then irradiated using a CO2 laser. The parameters of laser power and beam scanning speed were evaluated using scanning electron microscopy and X-ray diffraction. The results indicated that the optimal laser scanning speed and power for achieving the best metallurgical bonding between the substrate/BC and the BC-TGO/TC layers were 70 mm/s at 100 W and 550 mm/s at 70 W, respectively. Laser-based layer formation has proven to be a promising technique for the application of TBC. Full article
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<p>Condition of NiCrAlY layer formation on Ti-6Al-4V with increasing CO<sub>2</sub> laser irradiation speed.</p>
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<p>Images obtained by SEM of the NiCrAlY layer on Ti-6Al-4V by CO<sub>2</sub> laser: (<b>A</b>) 20 mm/s; (<b>B</b>) 40 mm/s; (<b>C</b>) 70 mm/s; (<b>D</b>) 80 mm/s; (<b>E</b>) 120 mm/s; (<b>F</b>) 140 mm/s.</p>
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<p>EDX analysis of the NiCrAlY layer on Ti-6Al-4V by CO<sub>2</sub> laser irradiation at a speed of 70 mm/s: (<b>A</b>) Result of in-line EDX analysis for the cross-section. (<b>B</b>) EDX map for the top surface of the sample.</p>
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<p>X-ray diffractograms of NiCrAlY powder: NiCrAlY layer on Ti-6Al-4V by CO<sub>2</sub> laser irradiation at 70 mm/s and after thermal treatment.</p>
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<p>Images obtained by SEM of the TBC coating with ZrYNb-16 ceramic deposition with 550 mm/s of CO<sub>2</sub> laser irradiation speed: (<b>A</b>) 100 W; (<b>B</b>) 70 W; (<b>C</b>) 60 W.</p>
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<p>EDX analysis on TBC sample map with ZrYNb-16 ceramic with 550 mm/s irradiation velocity and 70 W CO<sub>2</sub> laser power.</p>
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<p>X-ray diffractograms of ZrYNb-16 and TBC ceramic powder.</p>
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17 pages, 5024 KiB  
Article
Comparative Study of γ Radiation-Induced Effects on Fiber Bragg Gratings by Femtosecond Laser Point-by-Point Method and Line-by-Line Method
by Mingyang Hou, Yumin Zhang, Xin Xiong and Lianqing Zhu
Photonics 2025, 12(1), 32; https://doi.org/10.3390/photonics12010032 - 3 Jan 2025
Viewed by 419
Abstract
In the realm of advanced optical fiber sensing (OFS) technologies, Fiber Bragg Grating (FBG) has garnered widespread application in the monitoring of temperature, strain, and external refractive indices, particularly within high-radiation environments such as high-energy physics laboratories, nuclear facilities, and space satellites. Notably, [...] Read more.
In the realm of advanced optical fiber sensing (OFS) technologies, Fiber Bragg Grating (FBG) has garnered widespread application in the monitoring of temperature, strain, and external refractive indices, particularly within high-radiation environments such as high-energy physics laboratories, nuclear facilities, and space satellites. Notably, FBGs inscribed using femtosecond lasers are favored for their superior radiation resistance. Among various inscription techniques, the point-by-point (PbP) and line-by-line (LbL) methods are predominant; however, their comparative impacts on radiation durability have not been adequately explored. In this research, FBGs were inscribed on a single-mode fiber using both the PbP and LbL methods, and subsequently subjected to a total irradiation dose of 5.04 kGy (radiation flux of 2 rad/s) over 70 h in a 60Co-γ radiation environment. By evaluating the changes in temperature- and strain-sensing performance of the FBG pre-irradiation and post-irradiation, this study identifies a more favorable technique for writing anti-irradiation FBG sensors. Moreover, an analysis into the radiation damage mechanisms in optical fibers, alongside the principles of femtosecond laser inscription, provides insights into the enhanced radiation resistance observed in femtosecond laser-written FBGs. This study thus furnishes significant guidance for the development of highly radiation-resistant FBG sensors, serving as a critical reference in the field of high-performance optical fiber sensing technologies. Full article
(This article belongs to the Special Issue Emerging Trends in Optical Fiber Sensors and Sensing Techniques)
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<p>Schematic diagram of FBG sensing principle.</p>
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<p>Schematic of femtosecond inscription FBG.</p>
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<p>Micrograph of writing FBG by femtosecond laser.</p>
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<p>Schematic of sensing experiment.</p>
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<p>Sensing experiment setup.</p>
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<p>Schematic of radiation experiment.</p>
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<p>Temperature-sensing spectral diagram and central wavelength drift diagram written by the PbP method before irradiation: (<b>a</b>) 3d spectrum of heating; (<b>b</b>) center wavelength drift diagram of heating; (<b>c</b>) 3d spectrum of cooling; (<b>d</b>) center wavelength drift diagram of cooling.</p>
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<p>Temperature-sensing spectral diagram and central wavelength drift diagram written by the PbP method after irradiation: (<b>a</b>) 3d spectrum of heating; (<b>b</b>) center wavelength drift diagram of heating; (<b>c</b>) 3d spectrum of cooling; (<b>d</b>) center wavelength drift diagram of cooling.</p>
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<p>The strain-sensing spectrum and the central wavelength drift diagram written by the PbP method before irradiation: (<b>a</b>) 3d spectrum of applying strain; (<b>b</b>) center wavelength drift diagram of applying strain; (<b>c</b>) 3d spectrum of unloading strain; (<b>d</b>) center wavelength drift diagram of unloading strain.</p>
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<p>The strain-sensing spectrum and the central wavelength drift diagram written by the PbP method after irradiation: (<b>a</b>) 3d spectrum of applying strain; (<b>b</b>) center wavelength drift diagram of applying strain; (<b>c</b>) 3d spectrum of unloading strain; (<b>d</b>) center wavelength drift diagram of unloading strain.</p>
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10 pages, 1375 KiB  
Article
Quantifying Uncertainty in Laser-Induced Damage Threshold for Cylindrical Gratings
by Yuan Li, Junqi Xu, Guoliang Yang, Lihong Yang and Junhong Su
Micromachines 2025, 16(1), 45; https://doi.org/10.3390/mi16010045 - 30 Dec 2024
Viewed by 459
Abstract
The laser-induced damage threshold (LIDT) is a key measure of an optical component’s resistance to laser damage, making its accurate determination crucial. Following the ISO 21254 standards, we studied the measurement strategy and uncertainty fitting method for laser damage, establishing a calculation model [...] Read more.
The laser-induced damage threshold (LIDT) is a key measure of an optical component’s resistance to laser damage, making its accurate determination crucial. Following the ISO 21254 standards, we studied the measurement strategy and uncertainty fitting method for laser damage, establishing a calculation model for uncertainty. Research indicates that precise LIDT measurement can be achieved by using a small energy level difference and conducting multiple measurements. The LIDT values for the cylindrical grating are 15.34 ± 0.00052 J/cm2 (95% confidence) and 15.34 ± 0.00078 J/cm2 (99% confidence), demonstrating low uncertainty and reliable results. This strategy effectively measures the LIDT and uncertainty of various grating surface shapes, offering reliable data for assessing their anti-laser-damage performance. Full article
(This article belongs to the Section A:Physics)
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<p>SEM images of the optical element with periodic cylindrical structured surfaces. (<b>a</b>) Top view; (<b>b</b>) profile view.</p>
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<p>Schematic diagram of the laser-induced damage test system.</p>
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<p>Typical morphology of damaged spots.</p>
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<p>The result of the LIDT measurement for the sample.</p>
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21 pages, 2472 KiB  
Article
A Randomized, Double-Blind, Placebo-Controlled Study Evaluating a Novel Skin Care Cream with Olea europaea Stem Cell Extract Following Nd:YAG 1064 nm Laser Epilation
by Vassiliki Gardiki, Athanasia Varvaresou, Spyridon Papageorgiou, Evangelia Protopapa, Panagoula Pavlou, Efstathios Rallis, Apostolos Papadopoulos and Dimitrios Chaniotis
Cosmetics 2025, 12(1), 2; https://doi.org/10.3390/cosmetics12010002 - 30 Dec 2024
Viewed by 599
Abstract
The European Society for Laser Dermatology (ELSD) has established recommendations for safe and effective photo epilation; however, short-term common adverse effects occur as a result of laser treatment, such as edema and perifollicular erythema. Post-inflammatory hyperpigmentation also appears in certain skin types. Very [...] Read more.
The European Society for Laser Dermatology (ELSD) has established recommendations for safe and effective photo epilation; however, short-term common adverse effects occur as a result of laser treatment, such as edema and perifollicular erythema. Post-inflammatory hyperpigmentation also appears in certain skin types. Very few clinical studies have been conducted on the topical application of cosmetic skin care products aimed at decreasing the adverse effects on the skin epidermis following laser-assisted epilation procedures. Stem cells are found in plant and animal organisms and are responsible for the growth and restoration of damaged tissues. Plant stem cells divide throughout the life of the plant, creating new plant parts. Our aim was to develop a new cosmetic cream to decrease the intensity of some of the side effects of laser epilation and thus reduce the administration of topical medication. We developed a formulation with the active substance Olea europaea (Olive) Callus Culture Lysate (OLEA VITAE™ 02), which is derived from plant stem cells of the Mediterranean wild variety of Olea europaea, for application following laser epilation with an Nd:YAG 1064 nm laser. The new skin care cream was tested for its physicochemical and microbiological stability, according to the European Pharmacopoeia. The impacts of this substance on the potential side effects of Nd:YAG 1064 nm application, i.e., trans-epidermal water loss, keratin hydration, melanin, erythema, and skin elasticity, in comparison with the appropriate placebo, were investigated using biophysical measurements and a self-assessment questionnaire. Skin biopsies were also performed to evaluate the influence of the procedure and the application of the products on the epidermis and papillary dermis thickness. According to our findings, the incorporation of the plant stem cell extract of Olea europaea into our cream resulted in a stable cream with an appealing texture. Furthermore, the activity of erythema and hyperpigmentation was decreased when the cream was applied after Nd:YAG 1064 nm laser epilation. Full article
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<p>CONSORT 2010 FLOW DIAGRAM 1. Flow chart of participation in the self-assessment questionnaire study. * Health issues independent from the study.</p>
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<p>CONSORT 2010 FLOW DIAGRAM 2. Flow chart of participation in biophysical measurements and skin biopsies. * Personal reasons and health issues independent from the study.</p>
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<p>Test for the efficacy of antimicrobial evaluation according to Eur. Pharm. for the OLEA VITAE™ 02 cream. Subfigures (<b>a</b>–<b>e</b>): log reduction charts for (<b>a</b>). <span class="html-italic">Staphylococcus aureus</span>, (<b>b</b>). <span class="html-italic">Escherichia coli</span>, (<b>c</b>). <span class="html-italic">Pseudomonas aeruginosa</span>, (<b>d</b>). <span class="html-italic">Candida albicans</span>, (<b>e</b>). <span class="html-italic">Aspergillus brasiliensis</span>.</p>
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<p>Test for the efficacy of antimicrobial evaluation according to Eur. Pharm. for the OLEA VITAE™ 02 cream. Subfigures (<b>a</b>–<b>e</b>): log reduction charts for (<b>a</b>). <span class="html-italic">Staphylococcus aureus</span>, (<b>b</b>). <span class="html-italic">Escherichia coli</span>, (<b>c</b>). <span class="html-italic">Pseudomonas aeruginosa</span>, (<b>d</b>). <span class="html-italic">Candida albicans</span>, (<b>e</b>). <span class="html-italic">Aspergillus brasiliensis</span>.</p>
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<p>Mean percentage change in the measured values (D0–D14) for the tested creams OLEA VITAE™ 02 (referred to as OLEA in <a href="#cosmetics-12-00002-f004" class="html-fig">Figure 4</a>) and the placebo.</p>
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<p>Representative hematoxylin/eosin-stained skin tissue punch biopsies from the placebo and OLEA VITAE™ 02 cream groups, respectively (<b>A</b>,<b>C</b>). Representative Masson trichrome-stained skin tissue punch biopsies from untreated and treated volunteers (<b>B</b>,<b>D</b>). Images (<b>A</b>,<b>B</b>) represent the same case (case, coded VG10.1) as (<b>C</b>,<b>D</b>) (case, coded VG7.2). All magnifications are 4×. Scale bars of 0.1 mm are shown. Epidermis depth was calculated in multiple random positions and is shown in (<b>A</b>,<b>C</b>).</p>
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<p>Self-assessment questionnaire: Comparison of the effectiveness between the groups.</p>
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18 pages, 9596 KiB  
Article
Poly(Acrylic Acid)/TiO2 Nanocomposite Hydrogels for Paper Artwork Cleaning and Protection
by Sabina Botti, Francesca Bonfigli, Rosaria D’Amato, Jasmine Rodesi and Maria Gabriella Santonicola
Molecules 2025, 30(1), 75; https://doi.org/10.3390/molecules30010075 - 28 Dec 2024
Viewed by 407
Abstract
Paper-based artworks are prone to natural aging processes driven by chemical and biological processes. Numerous treatments have been developed to mitigate deterioration and prevent irreversible damage. In this study, we investigated the use of poly(acrylic acid)/TiO2 composite hydrogels, combining their cleaning and [...] Read more.
Paper-based artworks are prone to natural aging processes driven by chemical and biological processes. Numerous treatments have been developed to mitigate deterioration and prevent irreversible damage. In this study, we investigated the use of poly(acrylic acid)/TiO2 composite hydrogels, combining their cleaning and protective functions in a minimally invasive treatment. Hydrogels allow for controlled water flow and photocatalytic TiO2 nanoparticles enhance the hydrogel’s efficacy by enabling the removal of oxidation products and inactivating biological contaminants. Furthermore, this innovative material can act as a protective coating against UV-induced aging, preserving both color and stability of the paper. Raman spectroscopy and confocal laser scanning microscopy imaging techniques were employed to evaluate the treatments, allowing for us to differentiate between hydrolytic and oxidative aging processes. Our findings demonstrate that papers coated with poly(acrylic acid)/TiO2 composite hydrogels exhibit significant reductions in oxidative markers, an enhanced color stability, and an overall improved resistance to degradation compared to uncoated samples. Full article
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<p>Optical images in bright field of XIX paper. The green rectangle indicates the area of 200 µm × 200 µm scanned with a step size of 5 µm in Raman spectral imaging mode using green excitation (λ = 532 nm) and 10X objective (<b>a</b>) before the cleaning treatment; (<b>b</b>) after the cleaning treatment; (<b>c</b>) examples Raman spectra acquired before and after the cleaning treatment.</p>
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<p>Optical images of XIX paper (<b>upper</b> panels) and <span class="html-italic">O</span><sub>T</sub> marker value colocalized maps. (<b>a</b>) Before and (<b>b</b>) after 1 h CBP cleaning treatment. The color code is blue, green, red, yellow for increasing value of <span class="html-italic">O</span><sub>T</sub> marker.</p>
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<p>(<b>a</b>) Ageing marker variation in percentage for the XIX century paper, (<b>b</b>) the same for the XXI century paper. This laser printer 2021 paper was exposed to ambient light for three years.</p>
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<p>CLSM images (objective 4×) of a selected spot on Brehm paper. (<b>a</b>) Before cleaning treatment; (<b>b</b>) after cleaning treatment with CBP/TiO<sub>2</sub>. The figures show red and the green fluorescence channels and their overlay in yellow color (<b>c</b>) Graph of the red fluorescence signal intensity profiles detected along the yellow arrows of the spot before and after CBP/TiO<sub>2</sub> treatment.</p>
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<p>(<b>a</b>) Raman spectra of CBP/TiO<sub>2</sub> coated XIX century paper (red curve), XIX century paper sample after gel removal (light green curve), XIX century paper (green curve), and CBP/TiO<sub>2</sub> gel (brown curve); (<b>b</b>) score plot related to map of CBP/TiO<sub>2</sub> coated XIX century paper (red diamonds); CBP/TiO<sub>2</sub> coated XIX century paper after a first run of gel removal (orange dots) and a second run of gel removal (green squares). Ellipses are only a guide for the eyes.</p>
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<p>Optical images of 2024 modern paper: (<b>a</b>) uncoated and non-irradiated; (<b>b</b>) coated with a thin layer of CBP/TiO<sub>2</sub>; (<b>c</b>) uncoated and UVA irradiated; (<b>d</b>) coated with a thin layer of CBP/TiO<sub>2</sub> and UVA irradiated.</p>
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<p>Red–green–blue value distributions of 2024 modern paper comparing (<b>a</b>) uncoated (solid lines) and CBP/TiO<sub>2</sub> coated paper (dashed line); (<b>b</b>) uncoated paper (solid lines) and uncoated paper UVA-irradiated (dotted lines); (<b>c</b>) uncoated paper (solid lines), uncoated UVA-irradiated paper (dotted lines), CBP/TiO<sub>2</sub> coated UVA-irradiated paper (brown, light green, and cyan solid lines). (<b>d</b>) Magnification of red value distribution of uncoated paper (solid line), uncoated UVA-irradiated paper (dotted line), uncoated UVA irradiated paper (brown solid line).</p>
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<p>(<b>a</b>) Raman spectra acquired from 2024 paper before and after 31 h of UVA irradiation. (<b>b</b>,<b>c</b>) <span class="html-italic">C</span><sub>I</sub> marker value maps of uncoated 2024 paper non-irradiated and UVA-irradiated, respectively. (<b>d</b>) Raman spectra acquired from 2024 paper CBP/TiO<sub>2</sub> coated before and after 31 h of UVA irradiation. (<b>e</b>,<b>f</b>) <span class="html-italic">C</span><sub>I</sub> marker value maps of CBP/TiO<sub>2</sub> coated 2024 paper, non-irradiated and UVA-irradiated, respectively.</p>
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<p>(<b>a</b>,<b>b</b>) O<sub>T</sub> marker value maps of uncoated 2024 paper before and after 31 h of UVA irradiation, respectively. (<b>c</b>,<b>d</b>) O<sub>T</sub> marker value maps of CBP/TiO<sub>2</sub> coated 2024 paper before and after 31 h of UVA irradiation, respectively.</p>
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<p>(<b>a</b>) Marker variation in percentage after 31 h of UVA irradiation for the uncoated and coated 2024 paper. (<b>b</b>) Score plots of coated paper before (blue points) and after irradiation (fuchsia points). The PC1 component is the CBP/TiO<sub>2</sub> gel Raman spectrum, while PC2 component is the paper one.</p>
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<p>Optical images of 2021 paper naturally aged in laboratory: (<b>a</b>) uncoated; (<b>b</b>) coated with a thin layer of CBP/TiO<sub>2</sub>. (<b>c</b>) Red value distributions of 2021 paper naturally aged in laboratory: uncoated paper (solid line), CBP/TiO<sub>2</sub> coated paper (dashed line), uncoated UVA-irradiated paper (dotted line), CBP/TiO<sub>2</sub> coated UVA-irradiated paper.</p>
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<p>O<sub>T</sub> marker value maps for (<b>a</b>) uncoated paper, (<b>b</b>) UVA-irradiated uncoated paper, (<b>c</b>) coated paper, (<b>d</b>) UVA-irradiated coated paper. (<b>e</b>) Marker variation in percentage after 31 h of UVA irradiation for the uncoated and CBP/TiO<sub>2</sub> coated 2021 paper exposed to ambient light of laboratory for three years.</p>
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<p>CLSM images obtained in reflection mode of the 2021 paper: (<b>a</b>) uncoated; (<b>b</b>) CBP/TiO<sub>2</sub>-coated; (<b>c</b>) the intensity profiles measured along the yellow arrow; (<b>d</b>) marker variation percentage after 30 and 60 min of outdoor sun irradiation.</p>
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<p>Photo of hydrogel. On the left CBP gel (1–2–3) on the right CBP/TiO<sub>2</sub> gel (4–5–6).</p>
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17 pages, 6981 KiB  
Article
Influence of Different Spot Pattern Lasers on Cleaning Effect of TC4 Titanium Alloy
by Xinqiang Ma, Tengchao Liu, Yuan Ren, Yanlu Zhang, Zifa Xu, Wei Cheng, Zhenzhen Zhang, Yongmei Zhu and Qinhe Zhang
Materials 2025, 18(1), 61; https://doi.org/10.3390/ma18010061 - 27 Dec 2024
Viewed by 418
Abstract
This study employed different spot pattern lasers to clean the oxide film on the surface of a TC4 titanium alloy. The variation in temperature field and ablation depth during the laser cleaning process was simulated by establishing a finite element model. The effects [...] Read more.
This study employed different spot pattern lasers to clean the oxide film on the surface of a TC4 titanium alloy. The variation in temperature field and ablation depth during the laser cleaning process was simulated by establishing a finite element model. The effects of various laser processing parameters on the micromorphology, elemental composition, and surface roughness of the TC4 titanium alloy were analyzed. The results show that as the laser energy density increases, both the temperature field and ablation depth increase as well. Under optimal laser processing parameters, the laser energy density is 5.27 J/cm2, with a repetition frequency of 300 kHz and a scanning speed of 6000 mm/s. A comparison of the cleaning effects of Gaussian pulse lasers and Flat-top pulse lasers reveals that the Gaussian pulse laser causes less damage to the TC4 titanium alloy, resulting in lower oxygen content and roughness values after cleaning compared to Flat-top pulse laser cleaning. Full article
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<p>Micromorphology and composition of the TC4 titanium alloy oxide film: (<b>a</b>) SEM morphology at 250×; (<b>b</b>) EDS spectrum; and (<b>c</b>) thickness of the oxide film.</p>
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<p>Laser cleaning system and scanning path; (<b>a</b>) laser cleaning system; (<b>b</b>) Gaussian spot scanning path; (<b>c</b>) Flat-top spot scanning path.</p>
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<p>Geometry and mesh model for laser cleaning.</p>
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<p>Schematic diagram of thermal boundary conditions.</p>
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<p>Energy distribution of laser spots: (<b>a</b>) Gaussian spot; (<b>b</b>) circular Flat-top spot.</p>
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<p>Temperature field distribution: (<b>a</b>) Gaussian pulse laser; (<b>b</b>) Flat-top pulse laser.</p>
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<p>Temperature variation with time at different laser energy densities: (<b>a</b>) Gaussian pulse laser; (<b>b</b>) Flat-top pulse laser.</p>
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<p>Ablation depth of Gaussian pulse laser: (<b>a</b>) 4.52 J/cm<sup>2</sup>; (<b>b</b>) 4.90 J/cm<sup>2</sup>; (<b>c</b>) 5.27 J/cm<sup>2</sup>; (<b>d</b>) 5.65 J/cm<sup>2</sup>.</p>
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<p>Ablation depth of Flat-top pulse laser: (<b>a</b>) 4.52 J/cm<sup>2</sup>; (<b>b</b>) 4.90 J/cm<sup>2</sup>; (<b>c</b>) 5.27 J/cm<sup>2</sup>; (<b>d</b>) 5.65 J/cm<sup>2</sup>.</p>
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<p>Microstructure of Gaussian pulse laser at different laser energy densities. (<b>a</b>,<b>a1</b>) Before cleaning; (<b>b</b>,<b>b1</b>) 4.52 J/cm<sup>2</sup>; (<b>c</b>,<b>c1</b>) 4.90 J/cm<sup>2</sup>; (<b>d</b>,<b>d1</b>) 5.27 J/cm<sup>2</sup>; (<b>e</b>,<b>e1</b>) 5.65 J/cm<sup>2</sup>; (<b>f</b>,<b>f1</b>) 6.03 J/cm<sup>2</sup>.</p>
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<p>Microstructure of Flat-top pulse laser at different laser energy densities: (<b>a</b>,<b>a1</b>) Before cleaning; (<b>b</b>,<b>b1</b>) 4.52 J/cm<sup>2</sup>; (<b>c</b>,<b>c1</b>) 4.90 J/cm<sup>2</sup>; (<b>d</b>,<b>d1</b>) 5.27 J/cm<sup>2</sup>; (<b>e</b>,<b>e1</b>) 5.65 J/cm<sup>2</sup> (<b>f</b>,<b>f1</b>) 6.03 J/cm<sup>2</sup>.</p>
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<p>Schematic of laser cleaning of TC4 titanium alloy oxide film. (<b>a</b>) low fluence; (<b>b</b>) appropriate fluence; (<b>c</b>) high fluence.</p>
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<p>Energy spectrum analysis after Gaussian pulse laser cleaning. (<b>a</b>) Before laser cleaning; (<b>b</b>) 4.52 J/cm<sup>2</sup>; (<b>c</b>) 4.90 J/cm<sup>2</sup>; (<b>d</b>) 5.27 J/cm<sup>2</sup>; (<b>e</b>) 5.65 J/cm<sup>2</sup>; (<b>f</b>) 6.03 J/cm<sup>2</sup>.</p>
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<p>EDS analysis after cleaning with Flat-top pulse laser. (<b>a</b>) Before laser cleaning; (<b>b</b>) 4.52 J/cm<sup>2</sup>; (<b>c</b>) 4.90 J/cm<sup>2</sup>; (<b>d</b>) 5.27 J/cm<sup>2</sup>; (<b>e</b>) 5.65 J/cm<sup>2</sup>; (<b>f</b>) 6.03 J/cm<sup>2</sup>.</p>
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<p>Trends in elemental content variation. (<b>a</b>) Gaussian pulse laser; (<b>b</b>) Flat-top pulse laser.</p>
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<p>Surface Ra values under different laser energy densities. (<b>a</b>) Gaussian pulse laser; (<b>b</b>) Flat-top pulse laser.</p>
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16 pages, 4367 KiB  
Article
Laser Welding of Micro-Wire Stent Electrode as a Minimally Invasive Endovascular Neural Interface
by Bo Wen, Liang Shen and Xiaoyang Kang
Micromachines 2025, 16(1), 21; https://doi.org/10.3390/mi16010021 - 26 Dec 2024
Viewed by 533
Abstract
Minimally invasive endovascular stent electrodes are an emerging technology in neural engineering, designed to minimize the damage to neural tissue. However, conventional stent electrodes often rely on resistive welding and are relatively bulky, restricting their use primarily to large animals or thick blood [...] Read more.
Minimally invasive endovascular stent electrodes are an emerging technology in neural engineering, designed to minimize the damage to neural tissue. However, conventional stent electrodes often rely on resistive welding and are relatively bulky, restricting their use primarily to large animals or thick blood vessels. In this study, the feasibility is explored of fabricating a laser welding stent electrode as small as 300 μm. A high-precision laser welding technique was developed to join micro-wire electrodes without compromising structural integrity or performance. To ensure consistent results, a novel micro-wire welding with platinum pad method was introduced during the welding process. The fabricated electrodes were integrated with stent structures and subjected to detailed electrochemical performance testing to evaluate their potential as neural interface components. The laser-welded endovascular stent electrodes exhibited excellent electrochemical properties, including low impedance and stable charge transfer capabilities. At the same time, in this study, a simulation is conducted of the electrode distribution and arrangement on the stent structure, optimizing the utilization of the available surface area for enhanced functionality. These results demonstrate the potential of the fabricated electrodes for high-performance neural interfacing in endovascular applications. The approach provided a promising solution for advancing endovascular neural engineering technologies, particularly in applications requiring compact electrode designs. Full article
(This article belongs to the Section B:Biology and Biomedicine)
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<p>The simulation of the relative position design for the micro-wire stent electrode employed three different models to evaluate electrode configurations. (<b>A</b>,<b>B</b>): the blue “×” and disks represent the positions of the working electrodes. In models A and B, the grounding electrode is configured as a large rectangular pad measuring 7 <math display="inline"><semantics> <mrow> <mi mathvariant="normal">m</mi> <mi mathvariant="normal">m</mi> </mrow> </semantics></math> × 2 <math display="inline"><semantics> <mrow> <mi mathvariant="normal">m</mi> <mi mathvariant="normal">m</mi> </mrow> </semantics></math> × 30 <math display="inline"><semantics> <mrow> <mi mathvariant="normal">m</mi> <mi mathvariant="normal">m</mi> <mo>;</mo> </mrow> </semantics></math> (<b>C</b>,<b>D</b>): the 3D surface streamline potential maps display the potential distribution after electrical stimulation in each of the three models; (<b>E</b>,<b>F</b>): the potential distribution maps correspond to the working electrode plane, which is defined as a circular area with a radius of 10 mm.</p>
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<p>(<b>A</b>–<b>C</b>) illustrate the three-dimensional heat-affected zone and melt pool dimensions during laser welding on a platinum disk, whereas (<b>D</b>–<b>F</b>) present two-dimensional cross-sectional views of the melt pool. It is observed that with an increasing number of laser cycles, the melt pool size on the platinum disk progressively expands.</p>
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<p>The main steps (<b>A</b>–<b>D</b>) of the fabrication of the laser welding endovascular stent electrode.</p>
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<p>The images of the fabricated laser welding endovascular stent electrode. (<b>A</b>) present the microscopic image of the bonding effect of the laser welding electrode with the stent. (<b>B</b>) present the normal image of the bonding effect.</p>
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<p>(<b>A</b>,<b>B</b>) Averaged EIS at different processing parameters (n = 6). It indicates the optimal choice for laser ablating the micro-wire. The impedance decreases correspondingly with the decrease in laser energy and the increase in processing times. At the same time, increasing the laser cutting marking speed was equivalent to increasing the laser energy.</p>
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<p>The laser ablation image of the micro-wire under the optimal parameter.</p>
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<p>The laser welding image of the micro-wire with the platinum disk under the optimal parameter.</p>
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<p>Averaged EIS of the laser welding endovascular stent electrodes at different current intensity (n = 6) (<b>A</b>) present the impedance of the electrode after the laser welding. (<b>B</b>) present the phase angle of the electrode after laser welding.</p>
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<p>(<b>A</b>) Averaged CV curve of the electrode. (<b>B</b>) Averaged voltage transient curve of electrodes (n = 6).</p>
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21 pages, 3723 KiB  
Review
Advances in Deep Brain Imaging with Quantum Dots: Structural, Functional, and Disease-Specific Roles
by Tenesha Connor, Hemal Weerasinghe, Justin Lathia, Clemens Burda and Murat Yildirim
Photonics 2025, 12(1), 3; https://doi.org/10.3390/photonics12010003 - 24 Dec 2024
Viewed by 1026
Abstract
Quantum dots (QDs) have emerged as promising tools in advancing multiphoton microscopy (MPM) for deep brain imaging, addressing long-standing challenges in resolution, penetration depth, and light–tissue interactions. MPM, which relies on nonlinear photon absorption, enables fluorescence imaging within defined volumes, effectively reducing background [...] Read more.
Quantum dots (QDs) have emerged as promising tools in advancing multiphoton microscopy (MPM) for deep brain imaging, addressing long-standing challenges in resolution, penetration depth, and light–tissue interactions. MPM, which relies on nonlinear photon absorption, enables fluorescence imaging within defined volumes, effectively reducing background noise and photobleaching. However, achieving greater depths remains limited by light scattering and absorption, compounded by the need for balanced laser power to avoid tissue damage. QDs, nanoscale semiconductor particles with unique optical properties, offer substantial advantages over traditional fluorophores, including high quantum yields, large absorption cross-sections, superior photostability, and tunable emission spectra. These properties enhance signal to background ratio at increased depths and reduce scattering effects, making QDs ideal for imaging subcortical regions like the hippocampus without extensive microscope modifications. Studies have demonstrated the capability of QDs to achieve imaging depths up to 2100 μm, far exceeding that of conventional fluorophores. Beyond structural imaging, QDs facilitate functional imaging applications, such as high-resolution tracking of hemodynamic responses and neural activity, supporting investigations of neuronal dynamics and blood flow in vivo. Their stability enables long-term, targeted drug delivery and photodynamic therapy, presenting potential therapeutic applications in treating brain tumors, Alzheimer’s disease, and traumatic brain injury. This review highlights the impact of QDs on MPM, their effectiveness in overcoming light attenuation in deep tissue, and their expanding role in diagnosing and treating neurological disorders, positioning them as transformative agents for both brain imaging and intervention. Full article
(This article belongs to the Special Issue Emerging Trends in Multi-photon Microscopy)
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<p>Optical properties of various fluorophores used in deep brain imaging. (<b>A</b>) Comparison of absorption crosssection (η<span class="html-italic">σ<sub>3</sub></span>) for commercial QDs (Qtracker) and traditional dyes (Texas Red and SR101) across wavelength 1600 to 1840 nm. Adapted from [<a href="#B25-photonics-12-00003" class="html-bibr">25</a>]. (<b>B</b>) η<span class="html-italic">σ<sub>3</sub></span> for lab-created CdTe QDs, showing increased values with additional shell layers (CdSe, CdSe/ZnS) under 1600 nm excitation. Adapted from [<a href="#B36-photonics-12-00003" class="html-bibr">36</a>]. (<b>C</b>) Fluorescence decay comparison between common fluorophores and CdSe/ZnS QDs. Cy5 and Nile Red exhibit mono-exponential decays with lifetimes of 1.5 ns and 3.6 ns, respectively, while QD displays multi-exponential decay with mean lifetime of 10.3 ns, demonstrating significantly longer fluorescence duration. Adapted from [<a href="#B34-photonics-12-00003" class="html-bibr">34</a>].</p>
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<p>Structural multiphoton imaging (in vivo) of mouse brain vasculature at various depths using quantum dots (QDs) as fluorescent probe. (<b>A</b>) Images acquired at depths measured up to ~500 µm below dura-mater utilizing 2P modified microscopy systems comparing 800 nm NLO or 1050 nm OPO excitation in healthy mice intravenously injected with Qtracker655. Adapted from [<a href="#B44-photonics-12-00003" class="html-bibr">44</a>]. (<b>B</b>) Three-dimensional reconstruction of 3P imaging of vasculature labeled in red with Qtracker655 illustrating depths deep into the hippocampus (~2100 μm) obtained. Highlighted in green are third-harmonic generation (THG) signals outlining the white matter (860–100 μm below surface). Adapted from [<a href="#B25-photonics-12-00003" class="html-bibr">25</a>]. (<b>C</b>) The yellow color highlights mouse brain blood vessels labeled by QDs excited at 2200 nm. The cyan color shows THG imaging of white matter excited at 1700 nm. (<b>Left</b>): Three-dimensional reconstruction of 3P imaging of mouse vasculature labeled with Qtracker800 extending to ~1060 μm below surface. (<b>Right</b>): Three-dimensional reconstruction of 4P imaging of Qtracker655-labeled mouse vasculature extending to ~940 μm below surface. Adapted from [<a href="#B24-photonics-12-00003" class="html-bibr">24</a>]. (<b>D</b>) Schematic showing 3P imaging of PEGylated CdSe/5.8Cd/ZnS QD-labeled vasculature excited with 1600 nm in healthy mouse brain. Left: Schematic of mouse injected with QDs into the tail before brain imaging a craniotomy. A 3D reconstruction of the blood vessels shows imaging acquired without the skull achieved depths ~1550 μm. Fluorescence intensity and SBR measurements were also obtained at 1300 nm (SBR = 2.7) and 1550 nm (SBR = 1.7) imaging depths as shown in the two graphs below the schematic. Right: Schematic showing 3P imaging acquired through intact skull. Lower imagining depth is achieved though the skull (~850 μm). A 3D reconstruction of the vasculature is presented in the far right. Fluorescence intensity and SBR measurement were obtained at 850 μm (SBR = 2.3). Adapted from [<a href="#B26-photonics-12-00003" class="html-bibr">26</a>].</p>
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<p>QDs in various functional imaging applications in the brain. (<b>A</b>) In vivo 3PFM images of Qtracker655-labeled blood vessels at (<b>a</b>) 950 μm, (<b>c</b>) 1330 μm, (<b>e</b>) and 1660 μm below the brain surface. Green signal in (<b>a</b>) Shows myelinated axons. Line scans (<b>b</b>,<b>d</b>,<b>f</b>) along dashed lines measure blood flow speed. Pixel: 512 × 512. Adapted from [<a href="#B24-photonics-12-00003" class="html-bibr">24</a>]. (<b>B</b>) Simulated ΔF/F changes for cortical spike trans using QDs, VSDs, and GEVIs, assuming 105 indicators per ell with 100% membrane localization. Sampled at 10 kHz with excitation intensities of 100 mW/mm<sup>2</sup>. Adapted from [<a href="#B56-photonics-12-00003" class="html-bibr">56</a>]. (<b>C</b>) In vivo imaging of cortical stimulation with QD-JB1-C60 bioconjugates. (<b>Left</b>) Fluorescence at 575 ms shows four ROIs (A1–A4), with electrode position marked by white lines. (<b>Right</b>) ΔF/F% intensity profiles for ROI A1 across three mice, showing peak response (~2.1%) ~100 ms post-stimulation and return to baseline within 80–100 ms. Adapted from [<a href="#B50-photonics-12-00003" class="html-bibr">50</a>]. (<b>D</b>) Fluorescence images of neurons co-labeled with CdSe/ZnS-GSH QDs (green) and DiD (red), with merged images showing colocalization (yellow). DiD, a lipophilic dye embedded in the phospholipid bilayer, highlights cell membranes. The overlap of the images confirms the QDs correctly labeled the cell membranes of neurons. Adapted from [<a href="#B58-photonics-12-00003" class="html-bibr">58</a>]. (<b>E</b>) Flexible QD biointerfaces for photomodulation of neurons using NIR light. NIR light stimulates neurons in primary hippocampal neurons in vitro to generate safe capacitive ionic currents that generate action potentials. Adapted from [<a href="#B55-photonics-12-00003" class="html-bibr">55</a>].</p>
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<p>Schematic representations of synthesis methods and modifications utilized to create QDs for deep brain imaging and neuron tracking. (<b>A</b>) Depiction of four NIR QD synthesis methods, including (i) hot injection: sequential precursor addition at high temperature, (ii) heat up: gradual temperature increase after mixing all precursors, (iii) microwave: rapid heating via microwave for core growth, and (iv) hydrothermal: high-pressure, high-temperature growth in an autoclave. Adapted from [<a href="#B61-photonics-12-00003" class="html-bibr">61</a>]. (<b>B</b>) Depiction of band-gap engineering strategies to create specific band structure alignments in Cd/CdSe/ZnS QDs with a type II configuration, resulting in enhanced 3P fluorescence efficiency. Adapted from [<a href="#B36-photonics-12-00003" class="html-bibr">36</a>]. (<b>C</b>) Representation of a modular design for a targeted approach for QD-based bioconjugates to enhance membrane potential visualization with varying electron acceptor distances. Adapted from [<a href="#B50-photonics-12-00003" class="html-bibr">50</a>]. (<b>D</b>) Schematic illustration of various bioconjugation techniques: (<b>left</b>) biomolecules of interest and (<b>right</b>) surface coating approaches for QDs. Two main coating methods: amphiphilic polymer encapsulation (i,ii) and cap exchange with hydrophilic ligands (iii–v). Adapted from [<a href="#B75-photonics-12-00003" class="html-bibr">75</a>].</p>
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<p>Utilizing QDs for neurological applications in pathologies and disease. (<b>A</b>) Schematic illustration of the use of QDs for targeting imaging and therapy of brain tumors. QDs selectively accumulate in tumor cells, enabling their specific visualization with MPM, which can then be used therapeutically to induce targeted cell death. Designed using Biorender. (<b>B</b>) In vivo glioma imaging in rats 8 h post-injection with PEG-QDs (<b>left</b>) and NGR-PEG-QDs (<b>right</b>). Fluorescence is negligible with PEG-QDs but pronounced at the tumor site with NGR-PEG-QDs, enhancing glioma detection. Adapted from [<a href="#B91-photonics-12-00003" class="html-bibr">91</a>]. (<b>C</b>) Schematic of GQD-GPE treatment in APP/PS1 mice, targeting Aβ accumulation and neuroinflammation. GQDG administration led to Aβ clearance, reduced inflammation, and improved memory. Adapted from [<a href="#B97-photonics-12-00003" class="html-bibr">97</a>,<a href="#B98-photonics-12-00003" class="html-bibr">98</a>]. (<b>D</b>) Schematic representation of biofunctionalized QDs delivering drugs across the BBB via receptor-mediated transcytosis, targeting brain cancer cells. Once inside, QDs bind specific receptors (e.g., EGFR, VEGFR, folate) to release drugs and induce cytotoxicity. Adapted from [<a href="#B93-photonics-12-00003" class="html-bibr">93</a>].</p>
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19 pages, 833 KiB  
Review
Comparison of Surgical Interventions for Endometrioma: A Systematic Review of Their Efficacy in Addressing Infertility
by Alexandra Ioannidou, Nikolaos Machairiotis, Sofoklis Stavros, Anastasios Potiris, Theodoros Karampitsakos, Athanasios G. Pantelis and Petros Drakakis
Biomedicines 2024, 12(12), 2930; https://doi.org/10.3390/biomedicines12122930 - 23 Dec 2024
Viewed by 788
Abstract
Background: Endometriosis is characterized by the presence of endometrial tissue outside the uterus. Beyond medical treatment, surgical intervention is also a viable consideration. However, current guidelines do not clearly indicate whether laparoscopic cystectomy, ablative methods (CO2 laser vaporization, plasma energy), or [...] Read more.
Background: Endometriosis is characterized by the presence of endometrial tissue outside the uterus. Beyond medical treatment, surgical intervention is also a viable consideration. However, current guidelines do not clearly indicate whether laparoscopic cystectomy, ablative methods (CO2 laser vaporization, plasma energy), or sclerotherapy is the preferred option. Methods: We conducted searches in two databases (PubMed and Europe PMC) to retrieve articles containing the keywords ‘surgical intervention for Endometrioma, ovarian reserve, pregnancy rates, fertility’, published between 1 January 2000 and 31 December 2023. We included articles presenting information on surgical intervention for endometrioma and its correlation with infertility parameters. Articles describing conservative treatment were excluded. Data were extracted by two authors using predefined criteria. Results: The initial database search produced 1376 articles, which were narrowed down to 41 relevant articles meeting the eligibility criteria. Conclusions: Laparoscopic cystectomy appears to impact postoperative anti-mullerian hormone levels, showing a stronger correlation with larger cysts and individual factors. CO2 laser vaporization demonstrates favorable results compared to traditional cystectomy. Combining GnRH agonist treatment with assisted reproduction treatment after cystectomy could be considered an alternative method. Plasma energy causes less damage to ovarian function, with pregnancy outcomes comparable to cystectomy. Sclerotherapy shows promising results for ovarian reserve preservation, recurrence rates, and safety. Further studies comparing these techniques are necessary to provide guidance to clinicians. Full article
(This article belongs to the Special Issue Advanced Research in Endometriosis 4.0)
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<p>Study flowchart until final study selection.</p>
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16 pages, 55797 KiB  
Article
Er:YAG Laser Cleaning of Micromosaics from the Rosalinde and Arthur Gilbert Collection at the Victoria and Albert Museum
by Julia Brand, Carmen Vida and Lucia Burgio
Heritage 2024, 7(12), 7309-7324; https://doi.org/10.3390/heritage7120338 - 23 Dec 2024
Viewed by 489
Abstract
Conserving micromosaics from the Gilbert collection at the Victoria and Albert Museum is a challenge due to their complex structure and fragility. Their highly polished decorative surface prevents access to the substrate, yet deterioration can affect both, and manifests as cracking, failure and [...] Read more.
Conserving micromosaics from the Gilbert collection at the Victoria and Albert Museum is a challenge due to their complex structure and fragility. Their highly polished decorative surface prevents access to the substrate, yet deterioration can affect both, and manifests as cracking, failure and losses of the substrate and grouting, and as yellowing and accumulation of dirt on the uppermost organic coating. In order to minimise any possible damage to the substrate while cleaning the surface with solvents, laser cleaning using an Er:YAG laser was investigated on various micromosaic objects. Tests were first conducted on a non-displayable bonbonnière, and digital microscopy and scanning electron microscopy were performed to investigate the effects of the laser on the different materials; the results were then compared to solvent-cleaning only. The combination leading to the most effective cleaning results was found to be laser irradiation at fluences up to 0.71 J·cm−2 on the surface, followed by gentle swabbing with solvent. The surface was successfully cleaned with no changes induced on the materials, and a much lower amount of solvent was used. Following these successful preliminary tests, cleaning was undertaken on other pieces of the collection, and the outcome is presented in this study. The results show a great variability in terms of response of the materials to solvents, emphasising the need to consider each item on a case-by-case basis. Full article
(This article belongs to the Special Issue The Conservation of Glass in Heritage Science)
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<p>Cross-section of micromosaic with base or cassina material (stone, metal), putty paste in which glass <span class="html-italic">filati</span> are embedded, grouting around the <span class="html-italic">filati</span>, and several layers of wax on the surface.</p>
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<p>St. Peter’s Square bonbonnière (accession number LOAN:GILBERT.924-2008). (<b>a</b>) Overview of the bonbonniere with broken corner; (<b>b</b>) close-up on the broken corner showing the structure of the micromosaic with filati inserted in the putty; (<b>c</b>) broken fragment used for the trials. The structure of the micromosaic is clearly visible in the cross section, with the underlying putty into which the glass <span class="html-italic">filati</span> are inserted, and the grouting in between the <span class="html-italic">filati</span>.</p>
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<p>Before and after irradiation test on the fragment of the St. Peter’s Square bonbonnière. (<b>a</b>) Before; (<b>b</b>) after 3 laser shots at 0.56 J·cm<sup>−2</sup>, alteration circled in red; (<b>c</b>) close-up on the altered surface; (<b>d</b>) SEM image of the altered surface showing melting; (<b>e</b>) cross-section of the <span class="html-italic">filati</span> before laser irradiation; (<b>f</b>) cross-section of the <span class="html-italic">filati</span> after irradiation at 0.56 J·cm<sup>−2</sup> showing no change; (<b>g</b>) surface of the <span class="html-italic">filati</span> before laser irradiation; (<b>h</b>) surface of the <span class="html-italic">filati</span> after laser irradiation at 0.56 J·cm<sup>−2</sup> showing no change; (<b>i</b>) surface of the stone before laser irradiation; (<b>j</b>) surface of the stone after laser irradiation at 0.56 J·cm<sup>−2</sup> showing no change; (<b>k</b>) surface of the grouting before laser irradiation; (<b>l</b>) surface of the grouting after laser irradiation at 0.56 J·cm<sup>−2</sup> showing no change (slightly different spot, no changes in texture, inclusions).</p>
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<p>Before and after cleaning test on the bonbonnière. (<b>a</b>) Before laser and solvent cleaning; (<b>b</b>) after softening with the laser at 0.56 J·cm<sup>−2</sup> and swabbing with ethyl acetate. The red frame indicates cleaned area; (<b>c</b>) before solvent cleaning; and (<b>d</b>) after cleaning with ethyl acetate only.</p>
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<p>Microscopic images of the glass <span class="html-italic">filati</span> before and after treatment. (<b>a</b>) White <span class="html-italic">filati</span> of the sky before treatment; (<b>b</b>) white tiles after laser and solvent cleaning showing excellent removal of dirt and preservation of the tinted wax and no alteration of the <span class="html-italic">filati</span> or grouting; (<b>c</b>) white <span class="html-italic">filati</span> (different area) after solvent cleaning only showing good removal of dirt but dirt remaining at the corners.</p>
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<p><span class="html-italic">The Beautiful Sky of Italy</span> tabletop (accession number LOAN:GILBERT.894-2008) before treatment, showing dirt in the sky and concentric black cracks with losses.</p>
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<p>Using Evolon<sup>®</sup> to pre-wet the surface prior to irradiation. (<b>a</b>) The Evolon<sup>®</sup> following application. Note the yellow colour immediately picked up by the textile; (<b>b</b>) the three stages of cleaning: 1—Evolon<sup>®</sup> applied to the surface; 2—after removal of the Evolon<sup>®</sup> but before the laser; 3—after laser irradiation and swabbing.</p>
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<p>Images before and after treatment of the tabletop. (<b>a</b>) Overview before treatment; (<b>b</b>) overview after treatment showing good removal of dirt and brightening; (<b>c</b>) before treatment of the Milan and Genova sections; (<b>d</b>) after treatment of the Milan and Genova sections showing excellent removal of dirt and yellowing and brightening of colours; (<b>e</b>) before treatment, zoom on the Milan section; (<b>f</b>) after treatment showing better impression of 3D and brightening of colours.</p>
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<p>Microscopic images of the surface of the sky before and after cleaning. (<b>a</b>) Before cleaning, showing significant amount of dirt between <span class="html-italic">filati</span>; (<b>b</b>) after cleaning, showing reduction of the dirt, removal of the yellowing, and slight improvement around the crack; (<b>c</b>) before and after treatment of blue <span class="html-italic">filati</span>, showing good removal of dirt and preservation of the blue tinted wax; (<b>d</b>) before and after treatment of blue <span class="html-italic">filati</span> in a different area, showing good removal of dirt and preservation of the blue tinted wax.</p>
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<p>Microscopic images of the surface after treatment, checking for laser-induced damage; (<b>a</b>) close-up on the Milan duomo showing no alteration to the grouting or <span class="html-italic">filati</span>; (<b>b</b>) potential laser-induced damage on the dark green tower separating the Milan and Venice sections, showing possible laser damage circled in white dotted lines.</p>
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<p>Cleaning <span class="html-italic">The Flora of Two Sicilies</span> tabletop (LOAN:GILBERT.190-2008). (<b>a</b>) Overview of the tabletop; (<b>b</b>) a detail before cleaning; and (<b>c</b>) after cleaning.</p>
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<p>Successful examples of laser-assisted cleaning. (<b>a</b>) Bonbonnière depicting a mountain (LOAN:GILBERT.490-2008); (<b>b</b>) before and after cleaning image showing the difference between clean and dirty surface. (<b>c</b>) Bonbonnière depicting a goldfinch on a branch before cleaning (LOAN:GILBERT.203-2008) and (<b>d</b>) after cleaning; (<b>e</b>) microscopic image before treatment showing dirt between the <span class="html-italic">filati</span> and (<b>f</b>) after treatment showing reduction of dirt and improvement in the appearance of the dark cracks.</p>
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