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Search Results (6,011)

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Keywords = long-term dynamics

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28 pages, 3875 KiB  
Article
Machine Learning-Based Measurement and Prediction of Ground Settlement Induced by Shield Tunneling Undercrossing Existing Tunnels in Composite Strata
by Mei Dong, Mingzhe Guan, Kuihua Wang, Yeyao Wu and Yuhan Fu
Sensors 2025, 25(5), 1600; https://doi.org/10.3390/s25051600 - 5 Mar 2025
Abstract
To address the issue of insufficient accuracy in traditional settlement prediction methods for shield tunneling undercrossing in composite strata in Hangzhou, this paper proposes a particle swarm optimization (PSO)-based Bidirectional Long Short-Term Memory neural network (Bi-LSTM) prediction model for high-precision dynamic prediction of [...] Read more.
To address the issue of insufficient accuracy in traditional settlement prediction methods for shield tunneling undercrossing in composite strata in Hangzhou, this paper proposes a particle swarm optimization (PSO)-based Bidirectional Long Short-Term Memory neural network (Bi-LSTM) prediction model for high-precision dynamic prediction of ground settlement under small-sample conditions. Shield tunneling is a key method for urban tunnel construction. This paper presents the measurement and prediction of ground settlement caused by shield tunneling undercrossing existing tunnels in composite strata in Hangzhou. The longitudinal ground settlement curve resulting from shield tunnel excavation was analyzed using measured data, and the measured lateral ground settlement was compared with the Peck empirical formula. Using PSO, the performance of three machine learning models in predicting the maximum ground settlement at monitoring points was compared: Long Short-Term Memory neural network (LSTM), Gated Recurrent Unit neural network (GRU), and Bi-LSTM. The linear relationships between different input parameters and between input parameters and the output parameter were analyzed using the Pearson correlation coefficient. Based on this analysis, the model was optimized, and its prediction performance before and after optimization was compared. The results show that the Bi-LSTM model optimized with the PSO algorithm demonstrates superior performance, achieving both accuracy and stability. Full article
26 pages, 4471 KiB  
Article
The Efficacy of the New Energy Vehicle Mandate Policy on Passenger Vehicle Market in China
by Ning Wang, Xiufeng Li and Xuening Yang
World Electr. Veh. J. 2025, 16(3), 151; https://doi.org/10.3390/wevj16030151 - 5 Mar 2025
Abstract
This paper aims to assess the impact of the New Energy Vehicle (NEV) mandate policy on the passenger vehicle market in China, with a focus on its effectiveness in promoting NEV adoption. In response to global climate goals and energy security concerns, China [...] Read more.
This paper aims to assess the impact of the New Energy Vehicle (NEV) mandate policy on the passenger vehicle market in China, with a focus on its effectiveness in promoting NEV adoption. In response to global climate goals and energy security concerns, China has implemented various NEV policies, including the phase-out of direct subsidies and the introduction of the NEV mandate policy (dual-credits policy). This policy, which combines NEV credits and Corporate Average Fuel Consumption (CAFC) credits, aims not only to promote NEV adoption but also to support industrial policy objectives by helping the auto industry leapfrog traditional internal combustion engines and become globally competitive. In this study, a System Dynamics (SD) model was developed using Vensim software (10.2.2) to simulate interactions between automakers, government policies, and consumer behaviors. The results show that the NEV mandate policy significantly boosts NEV sales, with projections indicating that NEV sales will reach 15 million units by 2030, accounting for 55% of the passenger vehicle market. Additionally, the study finds that tightening NEV credits standards and increasing the NEV credit proportion requirements can further enhance market growth, with stricter measures post-2023 being crucial to achieving a 50% market share. In contrast, under a scenario where the dual-credits policy ends in 2024, the NEV market share would still grow but would fall short of the 50% target by 2030. The findings suggest that stronger policy measures will be essential to maintain long-term market momentum. Full article
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<p>Interaction Between CAFCs Policy and the Passenger Vehicle Market.</p>
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<p>Impact of NEV Credits Policy on the NEV and CV Markets.</p>
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<p>System Dynamics Model Framework for Assessing Dual-Credits Policy Impacts.</p>
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<p>Overall sales trend of passenger vehicles.</p>
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<p>Sales Trend of NEV in the Passenger Vehicle Market.</p>
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<p>Sales Trend of CV in the Passenger Vehicle Market.</p>
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<p>Market segment trend of BEV.</p>
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<p>Market segment trend of PHEV.</p>
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<p>Market segment trend of CV.</p>
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<p>NEV market share under different requirements of NEV credits ratio.</p>
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<p>NEV market share under different decline of NEV credits standard.</p>
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<p>NEV market share under mix scenarios.</p>
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<p>NEV market share under all scenarios.</p>
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23 pages, 587 KiB  
Review
Factors That Strengthen Community Resilience to Externally Initiated and Controlled Tourism in Post-Conflict Destinations: The Role of Amenity Migrants and Management Policies
by Seweryn Zielinski, Luz Helena Díaz Rocca and Young-joo Ahn
Land 2025, 14(3), 546; https://doi.org/10.3390/land14030546 - 5 Mar 2025
Abstract
This study examines community resilience in post-conflict tourism destinations of the Global South, where externally initiated and controlled tourism development often prevails. Using a conceptual research approach grounded in a comprehensive literature review, the paper identifies critical conditions for resilience-building in these fragile [...] Read more.
This study examines community resilience in post-conflict tourism destinations of the Global South, where externally initiated and controlled tourism development often prevails. Using a conceptual research approach grounded in a comprehensive literature review, the paper identifies critical conditions for resilience-building in these fragile contexts. It demonstrates that post-conflict tourism development typically unfolds in three stages: an initial phase of rapid growth driven by external stakeholders, followed by community awakening to tourism’s impacts, and culminating in community-led efforts to regain control. The study argues that even when initial tourism development exceeds local adaptive capacities, it can initiate a gradual process of resilience-building through proactive community action and supportive policies. The transformative potential of amenity migrants is emphasized, as they can shift from being stressors to becoming agents of change, fostering resilience, provided they are successfully integrated into local communities. The paper also advocates for longitudinal research to better understand the dynamics of amenity migrants’ assimilation and their role in resilience-building, particularly in the Global South, where empirical evidence remains limited. The findings provide valuable insights for designing strategies to achieve sustainable and inclusive tourism development in post-conflict and other vulnerable destinations, offering a pathway to empower local communities and foster long-term resilience. Full article
(This article belongs to the Special Issue The Role of Land Policy in Shaping Tourism Development)
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<p>Year of publication of used sources.</p>
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19 pages, 5973 KiB  
Article
Electric Vehicle Charging Load Forecasting Method Based on Improved Long Short-Term Memory Model with Particle Swarm Optimization
by Xiaomeng Yang, Lidong Zhang and Xiangyun Han
World Electr. Veh. J. 2025, 16(3), 150; https://doi.org/10.3390/wevj16030150 - 5 Mar 2025
Abstract
With the rapid global proliferation of electric vehicles (EVs), their integration as a significant load component within power systems increasingly influences the stable operation and planning of electrical grids. However, the high uncertainty and randomness inherent in EV users’ charging behaviors render accurate [...] Read more.
With the rapid global proliferation of electric vehicles (EVs), their integration as a significant load component within power systems increasingly influences the stable operation and planning of electrical grids. However, the high uncertainty and randomness inherent in EV users’ charging behaviors render accurate load forecasting a challenging task. In this context, the present study proposes a Particle Swarm Optimization (PSO)-enhanced Long Short-Term Memory (LSTM) network forecasting model. By combining the global search capability of the PSO algorithm with the advantages of LSTM networks in time-series modeling, a PSO-LSTM hybrid framework optimized for seasonal variations is developed. The results confirm that the PSO-LSTM model effectively captures seasonal load variations, providing a high-precision, adaptive solution for dynamic grid scheduling and charging infrastructure planning. This model supports the optimization of power resource allocation and the enhancement of energy storage efficiency. Specifically, during winter, the Mean Absolute Error (MAE) is 3.896, a reduction of 6.57% compared to the LSTM model and 10.13% compared to the Gated Recurrent Unit (GRU) model. During the winter–spring transition, the MAE is 3.806, which is 6.03% lower than that of the LSTM model and 12.81% lower than that of the GRU model. In the spring, the MAE is 3.910, showing a 2.71% improvement over the LSTM model and a 7.32% reduction compared to the GRU model. Full article
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<p>The three-dimensional spatial–temporal distribution of charging load in the winter season.</p>
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<p>The three-dimensional spatial–temporal distribution of charging load during the winter–spring transition period.</p>
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<p>The three-dimensional spatial–temporal distribution of charging load in the spring season.</p>
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<p>A structural diagram of the LSTM network.</p>
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<p>A diagram of the improved model structure.</p>
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<p>Diagram of particle global and historical optimal solutions, velocity, and position.</p>
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<p>The overall framework of the PSO-LSTM model.</p>
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<p>Winter charging load prediction comparison (1 January 2023–3 February 2023).</p>
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<p>Winter–spring transition charging load prediction comparison (4 February 2023–4 March 2023).</p>
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<p>Spring charging load prediction comparison (5 March 2023–29 April 2023).</p>
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20 pages, 6165 KiB  
Article
Prediction and Spatiotemporal Dynamics of Vegetation Index Based on Deep Learning and Environmental Factors in the Yangtze River Basin
by Yin Wang, Nan Zhang, Mingjie Chen, Yabing Zhao, Famiao Guo, Jingxian Huang, Daoli Peng and Xiaohui Wang
Forests 2025, 16(3), 460; https://doi.org/10.3390/f16030460 - 5 Mar 2025
Abstract
Accurately predicting the vegetation index (VI) of the Yangtze River Basin and analyzing its spatiotemporal trends are essential for assessing vegetation dynamics and providing recommendations for environmental resource management in the region. This study selected the key climate factors most strongly correlated with [...] Read more.
Accurately predicting the vegetation index (VI) of the Yangtze River Basin and analyzing its spatiotemporal trends are essential for assessing vegetation dynamics and providing recommendations for environmental resource management in the region. This study selected the key climate factors most strongly correlated with three vegetation indexes (VI): the Normalized Difference Vegetation Index (NDVI), Enhanced Vegetation Index (EVI), and kernel Normalized Difference Vegetation Index (kNDVI). Historical VI and climate data (2001–2020) were used to train, validate, and test a CNN-BiLSTM-AM deep learning model, which integrates the strengths of Convolutional Neural Networks (CNN), Bidirectional Long Short-Term Memory (BiLSTM), and Attention Mechanism (AM). The performance of this model was compared with CNN-BiLSTM, LSTM, and BiLSTM-AM models to validate its superiority in predicting the VI. Finally, climate simulation data under three Shared Socioeconomic Pathway (SSP) scenarios (SSP1-1.9, SSP2-4.5, and SSP5-8.5) were used as inputs to the CNN-BiLSTM-AM model to predict the VI for the next 20 years (2021–2040), aiming to analyze spatiotemporal trends. The results showed the following: (1) Temperature, precipitation, and evapotranspiration had the highest correlation with VI data and were used as inputs to the time series VI model. (2) The CNN-BiLSTM-AM model combined with the EVI achieved the best performance (R2 = 0.981, RMSE = 0.022, MAE = 0.019). (3) Under all three scenarios, the EVI over the next 20 years showed an upward trend compared to the previous 20 years, with the most significant growth observed under SSP5-8.5. Vegetation in the source region and the western part of the upper reaches increased slowly, while significant increases were observed in the eastern part of the upper reaches, middle reaches, lower reaches, and estuary. The analysis of the predicted EVI time series indicates that the vegetation growth conditions in the Yangtze River Basin will continue to improve over the next 20 years. Full article
(This article belongs to the Special Issue Mapping and Modeling Forests Using Geospatial Technologies)
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<p>Geographical location map of Yangtze River Basin, China (1: Source; 2: Upper reaches; 3: Middle reaches; 4: Lower reaches; 5: Estuary).</p>
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<p>The workflow (TMP: temperature; PRE: precipitation; ET: evapotranspiration; WD: windspeed; RH: relative humidity; SM: soil moisture).</p>
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<p>Trends in three climatic factors from 2021 to 2040 ((<b>A</b>): Temperature; (<b>B</b>): Precipitation; (<b>C</b>): Evapotranspiration).</p>
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<p>CNN-BiLSTM-AM model structure diagram.</p>
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<p>The spatial distribution of third-level river basins in the Yangtze River Basin.</p>
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<p>Results of Kolmogorov–Smirnov normality test for VIs and environmental factors.</p>
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<p>EVI prediction effects of CNN-BiLSTM-AM model.</p>
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<p>Temporal variation in EVI in Yangtze River Basin from 2001 to 2040.</p>
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<p>Spatial trends in the EVI from 2021 to 2040 under three SSP scenarios in the Yangtze River Basin. (<b>A</b>–<b>E</b>) represent EVI distribution in the years of 2021, 2021–2025, 2026–2030, and 2036–2040, respectively, under the SSPl-1.9 scenario; (<b>F</b>) represents the locations and periods with the maximum and minimum EVIs under the SSPl-1.9 scenario; (<b>G</b>–<b>K</b>) represent EVI distribution in the years of 2021, 2021–2025, 2026–2030, 2031–2035 and 2036–2040, respectively, under the SSP2-4.5 scenario; (<b>L</b>) represents the locations and periods with the maximum and minimum EVIs under the SSP2-4.5 scenario; (<b>M</b>–<b>Q</b>) represent EVI distribution in the years of 2021, 2021–2025, 2026–2030, and 2036–2040, respectively, under the SSP5-8.5 scenario; (<b>R</b>) represents the locations and periods with the maximum and minimum EVIs under the SSP5-8.5 scenario.</p>
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<p>Change rates in 67 sub-regions at five-year intervals relative to the previous five-year period ((<b>A</b>–<b>D</b>) represent the trends in the EVI for the periods of 2021–2025, 2026–2030, 2031–2035, and 2036–2040, respectively, under the SSP1-1.9 scenario; (<b>E</b>–<b>H</b>) represent the trends in the EVI for the periods of 2021–2025, 2026–2030, 2031–2035, and 2036–2040, respectively, under the SSP2-4.5 scenario; (<b>I</b>–<b>L</b>) represent the trends in the EVI for the periods of 2021–2025, 2026–2030, 2031–2035, and 2036–2040, respectively, under the SSP5-8.5 scenario).</p>
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17 pages, 299 KiB  
Article
A Spanish Dominican in Modern China: Manuel Prat Pujoldevall and His Mission
by Zhicang Huang
Religions 2025, 16(3), 325; https://doi.org/10.3390/rel16030325 - 5 Mar 2025
Abstract
The Catholic missions in early twentieth-century Xiamen represent a complex intersection between Western religious ambitions and the cultural intricacies of Southern Fujian, China. Here, the author examines the missionary work of Manuel Prat Pujoldevall, a Spanish Dominican active in Xiamen, including the Kulangsu [...] Read more.
The Catholic missions in early twentieth-century Xiamen represent a complex intersection between Western religious ambitions and the cultural intricacies of Southern Fujian, China. Here, the author examines the missionary work of Manuel Prat Pujoldevall, a Spanish Dominican active in Xiamen, including the Kulangsu International Settlement. Drawing on primary archival records and historical sources, this study assesses Prat’s strategies for governance, cultural adaptation, and resource allocation. The findings reveal that Prat’s pragmatic methods significantly influenced local community dynamics while highlighting the challenges he faced in reconciling religious objectives with shifting political and social conditions. Overall, this paper underscores that the long-term success of cross-cultural missionary work depends on a delicate balance between steadfast religious commitment and culturally adapted management, thereby contributing to broader discussions on the interplay between faith and culture in complicated historical contexts. Full article
25 pages, 20763 KiB  
Article
Research on Maneuvering Motion Prediction for Intelligent Ships Based on LSTM-Multi-Head Attention Model
by Dongyu Liu, Xiaopeng Gao, Cong Huo and Wentao Su
J. Mar. Sci. Eng. 2025, 13(3), 503; https://doi.org/10.3390/jmse13030503 - 5 Mar 2025
Abstract
In complex marine environments, accurate prediction of maneuvering motion is crucial for the precise control of intelligent ships. This study aims to enhance the predictive capabilities of maneuvering motion for intelligent ships in such environments. We propose a novel maneuvering motion prediction method [...] Read more.
In complex marine environments, accurate prediction of maneuvering motion is crucial for the precise control of intelligent ships. This study aims to enhance the predictive capabilities of maneuvering motion for intelligent ships in such environments. We propose a novel maneuvering motion prediction method based on Long Short-Term Memory (LSTM) and Multi-Head Attention Mechanisms (MHAM). To construct a foundational dataset, we integrate Computational Fluid Dynamics (CFD) numerical simulation technology to develop a mathematical model of actual ship maneuvering motions influenced by wind, waves, and currents. We simulate typical operating conditions to acquire relevant data. To emulate real marine environmental noise and data loss phenomena, we introduce Ornstein–Uhlenbeck (OU) noise and random occlusion noise into the data and apply the MaxAbsScaler method for dataset normalization. Subsequently, we develop a black-box model for intelligent ship maneuvering motion prediction based on LSTM networks and Multi-Head Attention Mechanisms. We conduct a comprehensive analysis and discussion of the model structure and hyperparameters, iteratively optimize the model, and compare the optimized model with standalone LSTM and MHAM approaches. Finally, we perform generalization testing on the optimized motion prediction model using test sets for zigzag and turning conditions. The results demonstrate that our proposed model significantly improves the accuracy of ship maneuvering predictions compared to standalone LSTM and MHAM algorithms and exhibits superior generalization performance. Full article
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<p>Define the ship’s coordinate system of motion.</p>
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<p>Turning motion data collection: (<b>a</b>) u in still water; (<b>b</b>) v in still water; (<b>c</b>) r in still water; (<b>d</b>) u in wave environment; (<b>e</b>) v in wave environment; and (<b>f</b>) r in wave environment.</p>
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<p>Zigzag motion data collection: (<b>a</b>) u in still water; (<b>b</b>) v in still water; (<b>c</b>) r in still water; (<b>d</b>) u in wave environment; (<b>e</b>) v in wave environment; and (<b>f</b>) r in wave environment.</p>
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<p>Zigzag motion data collection: (<b>a</b>) u in still water; (<b>b</b>) v in still water; (<b>c</b>) r in still water; (<b>d</b>) u in wave environment; (<b>e</b>) v in wave environment; and (<b>f</b>) r in wave environment.</p>
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<p>Training, validation, and testing sets.</p>
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<p>LSTM model unit structure.</p>
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<p>Multi-Head Attention Mechanism structure.</p>
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<p>LSTM-Multi-Head Attention-1 Model Framework.</p>
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<p>LSTM-Multi-Head Attention-2 Model Framework.</p>
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<p>LSTM-Multi-Head Attention-3 Model Framework.</p>
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<p>Forecasting effects of the proposed models.</p>
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<p>RMSE and loss curves of the proposed models.</p>
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<p>Forecasting effects of models with different regularization methods.</p>
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<p>RMSE and loss curves with different regularization methods.</p>
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<p>Forecasting effects of models with different numbers of heads.</p>
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<p>RMSE and loss curves with different numbers of heads.</p>
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<p>Analysis of the impact of the number of neurons on model performance.</p>
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<p>RMSE and loss curves with different number of neurons.</p>
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<p>Forecasting effects of models with different training batch sizes.</p>
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<p>RMSE and loss curves with different training batch sizes.</p>
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<p>Analysis of the Impact of sliding window size.</p>
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<p>RMSE and loss curves with different sliding window size.</p>
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<p>Comparison of prediction effects among LSTM, GRU, Multi-Head Attention, Transformer, and LSTM-Multi-Head Attention-2 models.</p>
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<p>RMSE and loss curves of LSTM, GRU, Multi-Head Attention, Transformer, and LSTM-Multi-Head Attention-2 models.</p>
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<p>Prediction of u, v, r, and heading for an 8-degree turning movement.</p>
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<p>Prediction of u, v, r, and heading for a 15-degree turning movement.</p>
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<p>Prediction of trajectory for 8-degree and 15-degree turning movement.</p>
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<p>Prediction of u, v, r, and heading for 5°/5° Zigzag.</p>
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<p>Prediction of u, v, r, and heading for 5°/5° Zigzag.</p>
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<p>The optimized forecasting effect.</p>
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23 pages, 960 KiB  
Article
Multivariate Air Quality Forecasting with Residual Nested LSTM Neural Network Based on DSWT
by Wangjian Li, Yiwen Zhang and Yaoyao Liu
Sustainability 2025, 17(5), 2244; https://doi.org/10.3390/su17052244 - 5 Mar 2025
Abstract
With the continuous deterioration of air quality and the increasingly serious environmental problem of air pollution, accurate air quality prediction is of great significance for environmental governance. Air quality index (AQI) prediction based on deep learning is currently a hot research topic. The [...] Read more.
With the continuous deterioration of air quality and the increasingly serious environmental problem of air pollution, accurate air quality prediction is of great significance for environmental governance. Air quality index (AQI) prediction based on deep learning is currently a hot research topic. The neural network model method currently used for prediction has difficulty effectively coping with the high volatility of AQI data and capturing the complex nonlinear relationships and long-term dependencies in the data. To address these issues, this paper proposes multivariate air quality forecasting with a residual nested LSTM neural network based on the discrete stationary wavelet transform (DSWT) model. Firstly, the DSWT data-decomposition technique decomposes each AQI data point into multiple sub-signals. Then, each sub-signal is sent to the NLSTM layer for processing to capture the temporal relationships between different pollutants. The processed results are then combined, using residual connections to mitigate issues of gradient vanishing and explosion during the model training process. The inverse mean squared error method is combined with the simple weighted average method, to serve as the weight-update approach. Back propagation is then applied, to dynamically adjust the weights based on the prediction accuracy of each sample, further enhancing the model’s prediction accuracy. The experiment was conducted on the air quality index dataset of 12 observation stations in and around Beijing. The results show that the proposed model outperforms several existing models and data-processing methods in multi-task AQI prediction. There were significant improvements in mean absolute error (MAE), root mean square error (RMSE), mean absolute percentage error (MAPE), and R square (R2). Full article
(This article belongs to the Section Air, Climate Change and Sustainability)
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<p>General flow chart.</p>
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<p>The flow chart for NLSTM.</p>
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<p>DSWT Residual NLSTM flow chart.</p>
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<p>Res NLSTM module.</p>
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20 pages, 7366 KiB  
Article
Histogram of Polarization Gradient for Target Tracking in Infrared DoFP Polarization Thermal Imaging
by Jianguo Yang, Dian Sheng, Weiqi Jin and Li Li
Remote Sens. 2025, 17(5), 907; https://doi.org/10.3390/rs17050907 - 4 Mar 2025
Abstract
Division-of-focal-plane (DoFP) polarization imaging systems have demonstrated considerable promise in target detection and tracking in complex backgrounds. However, existing methods face challenges, including dependence on complex image preprocessing procedures and limited real-time performance. To address these issues, this study presents a novel histogram [...] Read more.
Division-of-focal-plane (DoFP) polarization imaging systems have demonstrated considerable promise in target detection and tracking in complex backgrounds. However, existing methods face challenges, including dependence on complex image preprocessing procedures and limited real-time performance. To address these issues, this study presents a novel histogram of polarization gradient (HPG) feature descriptor that enables efficient feature representation of polarization mosaic images. First, a polarization distance calculation model based on normalized cross-correlation (NCC) and local variance is constructed, which enhances the robustness of gradient feature extraction through dynamic weight adjustment. Second, a sparse Laplacian filter is introduced to achieve refined gradient feature representation. Subsequently, adaptive polarization channel correlation weights and the second-order gradient are utilized to reconstruct the degree of linear polarization (DoLP). Finally, the gradient and DoLP sign information are ingeniously integrated to enhance the capability of directional expression, thus providing a new theoretical perspective for polarization mosaic image structure analysis. The experimental results obtained using a self-developed long-wave infrared DoFP polarization thermal imaging system demonstrate that, within the same FBACF tracking framework, the proposed HPG feature descriptor significantly outperforms traditional grayscale {8.22%, 2.93%}, histogram of oriented gradient (HOG) {5.86%, 2.41%}, and mosaic gradient histogram (MGH) {27.19%, 18.11%} feature descriptors in terms of precision and success rate. The processing speed of approximately 20 fps meets the requirements for real-time tracking applications, providing a novel technical solution for polarization imaging applications. Full article
(This article belongs to the Special Issue Recent Advances in Infrared Target Detection)
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<p>Feature extraction methods for DoFP polarization images: (<b>a</b>) indirect processing method based on calibration and demosaicking; (<b>b</b>) direct processing method based on mosaic images.</p>
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<p>Schematic diagram of the HPG feature descriptor.</p>
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<p>Long-wave infrared DoFP polarization mosaic dataset.</p>
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<p>Vehicle target tracking results with occlusion under foggy night conditions.</p>
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<p>Vehicle target tracking results with scale variations under clear night conditions.</p>
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<p>Precision and success plots of different feature extraction methods.</p>
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<p>Precision and success plots of different tracking methods: TECF [<a href="#B31-remotesensing-17-00907" class="html-bibr">31</a>], EMCF [<a href="#B30-remotesensing-17-00907" class="html-bibr">30</a>], AutoTrack [<a href="#B29-remotesensing-17-00907" class="html-bibr">29</a>], ARCF [<a href="#B28-remotesensing-17-00907" class="html-bibr">28</a>], GFS-DCF [<a href="#B27-remotesensing-17-00907" class="html-bibr">27</a>], ECO [<a href="#B26-remotesensing-17-00907" class="html-bibr">26</a>], and SRDCF [<a href="#B25-remotesensing-17-00907" class="html-bibr">25</a>].</p>
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22 pages, 6834 KiB  
Article
Regulatory Impacts of the Three Gorges Dam on Long-Term Terrestrial Water Storage Anomalies in the Three Gorges Reservoir Area: Insights from GRACE and Multi-Source Data
by Yu Zhang, Yi Zhang, Sulan Liu, Xiaohui Wu, Yubin Liu, Yulong Zhong and Yunlong Wu
Remote Sens. 2025, 17(5), 901; https://doi.org/10.3390/rs17050901 - 4 Mar 2025
Abstract
Understanding the impact of human activities on regional water resources is essential for sustainable basin management. This study examines long-term terrestrial water storage anomalies (TWSA) in the Three Gorges Reservoir Area (TGRA) over two decades, from 2003 to 2023. The analysis utilizes data [...] Read more.
Understanding the impact of human activities on regional water resources is essential for sustainable basin management. This study examines long-term terrestrial water storage anomalies (TWSA) in the Three Gorges Reservoir Area (TGRA) over two decades, from 2003 to 2023. The analysis utilizes data from the Gravity Recovery and Climate Experiment (GRACE) and its successor mission (GRACE-FO), complemented by Global Land Data Assimilation System (GLDAS) models and ECMWF Reanalysis v5 (ERA5) datasets. The research methodically explores the comparative contributions of natural factors and human activities to the region’s hydrological dynamics. By integrating the GRACE Drought Severity Index (GRACE-DSI), this study uncovers the dynamics of droughts during extreme climate events. It also reveals the pivotal role of the Three Gorges Dam (TGD) in mitigating these events and managing regional water resources. Our findings indicate a notable upward trend in TWSA within the TGRA, with an annual increase of 0.93 cm/year. This trend is largely due to the effective regulatory operations of TGD. The dam effectively balances the seasonal distribution of water storage between summer and winter and substantially reduces the adverse effects of extreme droughts on regional water resources. Further, the GRACE-DSI analysis underscores the swift recovery of TWSA following the 2022 drought, highlighting TGD’s critical role in responding to extreme climatic conditions. Through correlation analysis, it was found that compared with natural factors (correlation 0.62), human activities (correlation 0.91) exhibit a higher relative contribution to TWSA variability. The human-induced contributions were derived from the difference between GRACE and GLDAS datasets, capturing the combined effects of all human activities, including the operations of the TGD, agricultural irrigation, and urbanization. However, the TGD serves as a key regulatory facility that significantly influences regional water resource dynamics, particularly in mitigating extreme climatic events. This study provides a scientific basis for water resource management in the TGRA and similar large reservoir regions, emphasizing the necessity of integrating the interactions between human activities and natural factors in basin management strategies. Full article
(This article belongs to the Special Issue Remote Sensing in Natural Resource and Water Environment II)
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<p>Analysis flow chart of water resources reserve change in the TGRA.</p>
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<p>Location of the research area and related watersheds ((<b>a</b>) the location of the Yangtze River basin; (<b>b</b>) the location of the TGRA).</p>
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<p>Comparison of TWSA derived from GRACE, GRACE-FM, and GLDAS datasets for the TGRA from 2003 to 2023. R represents the rate of change in TWSA reflected by GRACE-FM.</p>
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<p>TWSA time series (2003–2023) for the TGRA derived from GRACE-FM, GLDAS, and ERA5, alongside precipitation data from 1 km monthly precipitation dataset for China.</p>
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<p>Seasonal variations in TWSA time series for the TGRA: (<b>a</b>) spring, (<b>b</b>) summer, (<b>c</b>) autumn, (<b>d</b>) winter.</p>
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<p>Detrended and deseasonalized TWSA series derived from GRACE-FM, GLDAS, and ERA5.</p>
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<p>Spatial distribution of TWSA in TGRA based on different data sources. (<b>a</b>,<b>c</b>,<b>e</b>,<b>g</b>) represent the spatial distribution results of TWSA in the TGRA for September 2021, calculated using GRACE, GRACE-FM, GLDAS, and ERA5, respectively; (<b>b</b>,<b>d</b>,<b>f</b>,<b>h</b>) represent the spatial distribution results of TWSA in the TGRA for September 2022, calculated using GRACE, GRACE-FM, GLDAS, and ERA5, respectively.</p>
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<p>Changes in TGD water level and TWSA from GRACE, GRACE-FM, and GLDAS. The solid black line represents the trend of GRACE-FM for three periods: the first period from July 2003 to October 2006, the second from October 2006 to August 2020, and the third from August 2020 to December 2023. The dashed black lines indicate the time points separating the three periods: the first is July 2003, the second is October 2006, and the third is August 2020. Milestones for water storage are annotated.</p>
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<p>Time series and correlation analysis of GRACE data with natural and human TWSA. Note that the correlation coefficient R has no units ((<b>a</b>) GRACE data and time series of natural TWSA; (<b>b</b>) Correlation analysis between GRACE data and natural TWSA; (<b>c</b>) GRACE data and time series of artificial TWSA; (<b>d</b>) Correlation analysis between GRACE data and human TWSA).</p>
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<p>Time series relationship between TGD water storage and human-driven contributions. The shaded areas are 2006, 2010, and 2022, corresponding to TGD’s trial operation phase, full operation phase, and an extreme drought event, respectively.</p>
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<p>GRACE-DSI time series changes from January 2003 to December 2023 in TGRA. The gray dashed line indicates the critical value of the “Near-Normal” state. Note that the drought index corresponds to its starting point and the indices are dimensionless.</p>
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<p>Spatial distribution of drought index in May, August, and November of TGRA in 2006, 2010, and 2022. Note that the indices are dimensionless.</p>
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18 pages, 4617 KiB  
Article
Real Option Valuation of an Emerging Renewable Technology Design in Wave Energy Conversion
by James A. DiLellio, John C. Butler, Igor Rizaev, Wanan Sheng and George Aggidis
Econometrics 2025, 13(1), 11; https://doi.org/10.3390/econometrics13010011 - 4 Mar 2025
Viewed by 39
Abstract
The untapped potential of wave energy offers another alternative to diversifying renewable energy sources and addressing climate change by reducing CO2 emissions. However, development costs to mature the technology remain significant hurdles to adoption at scale and the technology often must compete [...] Read more.
The untapped potential of wave energy offers another alternative to diversifying renewable energy sources and addressing climate change by reducing CO2 emissions. However, development costs to mature the technology remain significant hurdles to adoption at scale and the technology often must compete against other marine energy renewables such as offshore wind. Here, we conduct a real option valuation that includes the uncertain market price of wholesale electricity and managerial flexibility expressed in determining future optimal decisions. We demonstrate the probability that the project’s embedded compound real option value can turn a negative net present value wave energy project to a positive expected value. This change in investment decision uses decision tree analysis, where real options are developed as decision nodes, and models the uncertainty as a risk-neutral stochastic process using chance nodes. We also show how our results are analogous to a financial out-of-the-money call option. Our results highlight the distribution of outcomes and the benefit of a staged long-term investment in wave energy systems to better understand and manage project risk, recognizing that these probabilistic results are subject to the ongoing evolution of wholesale electricity prices and the stochastic process models used here to capture their future dynamics. Lastly, we show that the near-term optimal decision is to continue to fund ongoing development of a reference architecture to a higher technology readiness level to maintain the long-term option to deploy such a renewable energy system through private investment or private–public partnerships. Full article
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<p>Time series of UK wholesale electricity prices.</p>
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<p>Histogram of monthly returns of UK wholesale electricity prices, 2014–2023.</p>
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<p>Twelve-month rolling annualized volatility, 2014–2023.</p>
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<p>GBM process forecast for wholesale electricity prices.</p>
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<p>Climatological annual mean wave power, 1980–2021.</p>
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<p>The tailless TALOS (displacement: 2969 m<sup>3</sup>).</p>
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<p>Decision tree for compound option.</p>
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<p>Non-recombining lattice to model the uncertain value of the underlying project’s cash flows for site A.</p>
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<p>Policy tree for site A with an expected <span class="html-italic">NPV</span> of EUR 12.6 M.</p>
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<p>Policy tree for site B with an expected <span class="html-italic">NPV</span> of EUR 1.17 M.</p>
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14 pages, 17234 KiB  
Article
A Grid-Based Long Short-Term Memory Framework for Runoff Projection and Uncertainty in the Yellow River Source Area Under CMIP6 Climate Change
by Haibo Chu, Yulin Jiang and Zhuoqi Wang
Water 2025, 17(5), 750; https://doi.org/10.3390/w17050750 - 4 Mar 2025
Viewed by 57
Abstract
Long-term runoff projection and uncertainty estimates can provide both the changing trends and confidence intervals of water resources, provide basic information for decision makers, and reduce risks for water resource management. In this paper, a grid-based runoff projection and uncertainty framework was proposed [...] Read more.
Long-term runoff projection and uncertainty estimates can provide both the changing trends and confidence intervals of water resources, provide basic information for decision makers, and reduce risks for water resource management. In this paper, a grid-based runoff projection and uncertainty framework was proposed through input selection and long short-term memory (LSTM) modelling coupled with uncertainty analysis. We simultaneously considered dynamic variables and static variables in the candidate input combinations. Different input combinations were compared. We employed LSTM to develop a relationship between monthly runoff and the selected variables and demonstrated the improvement in forecast accuracy through comparison with the MLR, RBFNN, and RNN models. The LSTM model achieved the highest mean Kling–Gupta Efficiency (KGE) score of 0.80, representing respective improvements of 45.45%, 33.33%, and 2.56% over the other three models. The uncertainty sources originating from the parameters of the LSTM models were considered, and the Monte Carlo approach was used to provide uncertainty estimates. The framework was applied to the Yellow River Source Area (YRSR) at the 0.25° grid scale to better show the temporal and spatial features. The results showed that extra information about static variables can improve the accuracy of runoff projections. Annual runoff tended to increase, with projection ranges of 148.44–296.16 mm under the 95% confidence level, under various climate scenarios. Full article
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<p>Structural diagram for the LSTM-based framework, including input selection, LSTM modelling, and uncertainty analysis.</p>
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<p>Location of the research area.</p>
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<p>Heatmap of correlations between runoff and the candidate input variables.</p>
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<p>Performance of different input combinations (KGE). The KGE value is a comprehensive metric for evaluating model performance. The closer the value of KGE is to 1, the higher the accuracy of the LSTM model, representing a more accurate description and calculation of the relationship between precipitation, temperature, and historical runoff.</p>
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<p>Spatial distribution maps of R<sup>2</sup>, KGE, NSE, and RMSE during the training period.</p>
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<p>Spatial distribution maps of R<sup>2</sup>, KGE, NSE, and RMSE during the testing period.</p>
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<p>Time series of future runoff projections from YRSR under different SSPs (2016–2045).</p>
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<p>Distribution of future runoff projections in the YRSR under different SSPs.</p>
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<p>Boxplot of confidence intervals for monthly runoff from 2016 to 2045 for a grid.</p>
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<p>Distribution of runoff values at January 2030.</p>
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20 pages, 7066 KiB  
Brief Report
Managing the Microbiome on the Surface of Tomato Fruit by Treatment of Tomato Plants with Non-Thermal Atmospheric-Pressure Plasma During Cultivation
by Hideki Takahashi, Keisuke Takashima, Shuhei Miyashita, Shota Sasaki, Abebe Alemu Derib, Kazuhisa Kato, Yoshinori Kanayama and Toshiro Kaneko
Horticulturae 2025, 11(3), 276; https://doi.org/10.3390/horticulturae11030276 - 4 Mar 2025
Viewed by 56
Abstract
The treatment of plants with non-thermal atmospheric-pressure plasma impacts several aspects of plant life. However, the effects of long-term plasma irradiation on crop cultivation are not enough investigated. The purpose of the current study is to address this subject. The growth of tomato [...] Read more.
The treatment of plants with non-thermal atmospheric-pressure plasma impacts several aspects of plant life. However, the effects of long-term plasma irradiation on crop cultivation are not enough investigated. The purpose of the current study is to address this subject. The growth of tomato plants, the preservation status of harvested tomato fruits, and the microbial community on the surface of harvested tomato fruits were compared between 12 long-term plasma-irradiated plants and 12 air-irradiated plants with statistical analyses. The growth parameters (plant height, number of leaves and fruit bunches, SPAD value, and plant dry weight) of the plants that were periodically irradiated with plasma from the three-leaf stage to the green-enlarged-fruit stage, were the same as those of the air-irradiated controls. However, the preservation status of the tomato fruits harvested from the plasma-irradiated plants was improved in comparison with that of the fruits from the air-irradiated controls. Analysis of the microbiome on the surface of the fruit indicated that long-term plasma irradiation during cultivation promoted an increased bacterial diversity on the fruit surface. Thus, the effect of plasma irradiation on the diversification of microbial population dynamics on tomato fruit may be associated with an improved preservation status of harvested tomato fruits. Full article
(This article belongs to the Section Postharvest Biology, Quality, Safety, and Technology)
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<p>Timetable of tomato plant cultivation, non-thermal atmospheric-pressure plasma or air irradiation, analysis of plant growth, and evaluation of preservation status of harvested tomato fruits. Twelve tomato plants were periodically irradiated with plasma or air as a control from the three-leaf stage to the premature-green-fruit stage every Monday, Wednesday, and Friday. Measurements of plant height, numbers of leaves and fruit bundles, SPAD value, and dry weight were performed three times: the first experiment was conducted on June 4, 11, 18, and 25 in 2021 (Exp. 1); the second experiment was conducted on August 5, 12, 19, and 26 in 2022 (Exp. 2); and the third experiment was conducted on November 30 in 2022 and January 6, 13, and 20 in 2023 (Exp. 3). In these three experiments, green-enlarged premature fruits were harvested for evaluation of preservation status and microbiome analysis. At 2 weeks after harvesting, the status of the preserved fruits was photographed.</p>
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<p>Schematic of the lab-built PEGDS spray device for tomato plant. The PEGDS spray device was powered by rechargeable lithium-ion batteries, which provided 24V bus power to the air compressor, water pOk,ump, and dielectric barrier discharge (DBD) plasma generator developed in a previous work [<a href="#B28-horticulturae-11-00276" class="html-bibr">28</a>]. The plasma effluent gas from DBD plasma was transferred to the PEGDS spray nozzle to force its dissolution into the liquid and be sprayed toward ambient. The flows of plasma effluent gas and water were shown by red and blue arrows, respectively. To avoid any reactive species loss, the nozzle and gas tubes were made of PTFE or PFA.</p>
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<p>Efficacy of long-term plasma irradiation in influencing tomato plant growth. Twelve tomato plants were periodically irradiated with non-thermal atmospheric-pressure plasma or air as a control from the three-leaf stage to the premature-green-fruit stage every Monday, Wednesday, and Friday. On 4, 11, 18, and 25 June 2021, plant height (<b>A</b>), number of leaves (<b>B</b>), number of fruit bunches (<b>C</b>), and plant dry weight (<b>D</b>) were analyzed. The average of plant height, number of leaves, number of fruit bunches, and plant dry weight are shown in a bar chart with error bars (<span class="html-italic">n</span> = 12, SD). Statistical analysis did not denote significant differences between plasma treatment and air treatment as a control (Welch’s <span class="html-italic">t</span>-test, <span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Efficacy of long-term plasma irradiation on the chlorophyll content of tomato plant leaves. Twelve tomato plants were periodically irradiated with non-thermal atmospheric-pressure plasma or air as a control from the three-leaf stage to the premature-green-fruit stage every Monday, Wednesday, and Friday. The SPAD value, which indicates the amount of chlorophyll in the leaves, was measured on June 4, 11, 18, and 25 in 2021. The average of SPAD value is shown in a bar chart with error bars (<span class="html-italic">n</span> = 12, SD). Statistical analysis did not denote significant differences between plasma treatment and air treatment as a control (Welch’s <span class="html-italic">t</span>-test, <span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Photograph of tomato fruits harvested from non-thermal atmospheric-pressure plasma- or air-irradiated tomato plants in Exp. 1. Premature enlarged green tomato fruits immediately after they were harvested (<b>upper panel</b>) and mature red tomato fruits 2 weeks after harvesting (<b>lower panel</b>) were photographed.</p>
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<p>Quantitative measurement of the amounts of the V4 region of 16S rDNA and the ITS1 region of 18S rDNA. The amounts of V4 region DNA from 16S rDNA and ITS1 region from 18S rDNA is shown in (<b>A</b>) and (<b>B</b>), respectively. The mean amount of DNA calculated for each sample was shown by a bar chart with error bars (<span class="html-italic">n</span> = 12, SD). Statistical analysis did not denote significant differences between plasma treatment and air treatment as a control (Welch’s <span class="html-italic">t</span>-test, <span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Difference in the bacterial and fungal richness on the surface of tomato fruits harvested from non-thermal atmospheric-pressure plasma- and air-irradiated tomato plants. Shannon α-diversity of the bacterial community (<b>A</b>) and the fungal community (<b>B</b>) on the surface of premature green fruits harvested from plasma- and air (Control)-irradiated tomato plants are indicated by box-and-whisker plots. Mean of Shannon_entropy was calculated for each sample and is indicated by the horizontal line in each box. Error bars indicate the standard deviation around the mean. The statistical difference in Shannon α-diversity was assessed using the Kruskal-Wallis test. A significant difference is indicated by the asterisk (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Difference in β-diversity plots of the bacterial and fungal richness on the surface of tomato fruits harvested from non-thermal atmospheric-pressure plasma- or air-irradiated tomato plants. Weighted_Unifrac β-diversity of the bacterial community (<b>A</b>) and the fungal community (<b>D</b>) on the surface of premature green fruits harvested from plasma and air (Control)-irradiated tomato plants, is indicated by plots of the principal component analysis (PCA). β-diversity plots of plasma samples are indicated by black circles and β-diversity plots of air samples are indicated by white circles. Weighted_UniFrac distance of the plots within Control or Plasma to Control (<b>B</b>,<b>E</b>), and within Plasma or Control to Plasma (<b>C</b>,<b>F</b>), are indicated by box-and-whisker plots. The mean UniFrac distance was calculated for each sample and is indicated by the horizontal line in the box. Error bars indicate the standard deviation around the mean. The statistical difference in Weighted_Unifrac β-diversity was assessed using PERMANOVA. A significant difference is indicated by the asterisk (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Phytotron used for cultivation of tomato plants irradiated with non-thermal atmospheric-pressure plasma or air as a control. (<b>A</b>) Natural light-type phytotron with supplementary light devices. (<b>B</b>) Humidified air plasma device (<b>left</b>) and cultivated tomato plants (<b>right</b>).</p>
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<p>Photograph of harvested premature green tomato fruits soaked in 10 mL of sterilized distilled water in sealing bags. The bags were shaken at 120 rpm at 25 °C for 30 min.</p>
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<p>FTIR absorption spectrum of air plasma effluent gas obtained during the PEGDS device test.</p>
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<p>Measured Br<sub>3</sub><sup>−</sup><sub>aq</sub> absorption spectra for samples sprayed and collected at various distances from the spray nozzle exit. A NaBr solution was mixed with the sprayed and collected PEGDS after the specified retention times (<span class="html-italic">t<sub>r</sub></span><sub>ete</sub>).</p>
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<p>Efficacy of long-term plasma irradiation i influencing tomato plant growth in Exp. 2. Twelve tomato plants were periodically irradiated with non-thermal atmospheric-pressure plasma or air as a control from the three-leaf stage to the premature-green-fruit stage every Monday, Wednesday, and Friday. On 5, 12, 19 and 26 August 2022, the plant height (<b>A</b>), number of leaves (<b>B</b>), number of fruit bunches (<b>C</b>), and plant dry weight (<b>D</b>) were measured. The average of plant height, number of leaves, number of fruit bunches, and plant dry weight are shown in a bar chart with error bars (<span class="html-italic">n</span> = 12, SD). Statistical analysis did not denote significant differences between plasma treatment and air treatment as a control (Welch’s <span class="html-italic">t</span>-test, <span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Efficacy of long-term plasma irradiation in influencing tomato plant growth in Exp. 3. Twelve tomato plants were periodically irradiated with non-thermal atmospheric-pressure plasma or air as a control from the three-leaf stage to the premature-green-fruit stage every Monday, Wednesday, and Friday. On 30 November 2022 and on 6, 13 and 20 January 2023, the plant height (<b>A</b>), number of leaves (<b>B</b>), number of fruit bunches (<b>C</b>), and plant dry weight (<b>D</b>) were analyzed. The average of plant height, number of leaves, number of fruit bunches, and plant dry weight are shown in a bar chart with error bars (<span class="html-italic">n</span> = 12, SD). Statistical analysis did not denote significant differences between plasma treatment and air treatment as a control (Welch’s <span class="html-italic">t</span>-test, <span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Efficacy of long-term plasma irradiation in influencing the chlorophyll content of tomato plant leaves. Twelve tomato plants were periodically irradiated with non-thermal atmospheric-pressure plasma or air as a control from the three-leaf stage to the premature-green-fruit stage on every Monday, Wednesday, and Friday. On 5, 12, 19, and 26 August 2022, the SPAD value, which indicates the amount of chlorophyll in the leaves, was measured (upper row of the bar charts) as well as on 30 November 2022 and on 6, 13, and 20 January 2023 (lower row of the bar charts). The average SPAD values are shown in the bar charts with error bars (<span class="html-italic">n</span> = 12, SD). Statistical analysis did not denote significant differences between plasma treatment and air treatment as a control (Welch’s <span class="html-italic">t</span>-test, <span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Photographs of tomato fruits harvested from plasma- or air-irradiated tomato plants in Exp. 2. Premature enlarged green tomato fruits immediately after they were harvested (<b>upper panel</b>) and mature red tomato fruits 2 weeks after harvesting (<b>lower panel</b>) were photographed.</p>
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<p>Relative abundance bar chart of bacterial communities at the phylum level on the surface of tomato fruits harvested from plasma-irradiated plants. The bacterial communities on the surface of premature enlarged green tomato fruits harvested from six plasma-irradiated tomato plants are indicated by the red dashed line (<b>A</b>). The other bacterial communities were found on the surface of premature enlarged green tomato fruits harvested from six air-irradiated tomato plants as a control (<b>A</b>). Each bacterial communities at the phylum level is shown in panel B (<b>B</b>).</p>
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<p>Relative abundance bar chart of fungal communities at the phylum level on the surface of tomato fruits harvested from plasma-irradiated plants. The fungal communities on the surface of premature enlarged green tomato fruits harvested from six plasma-irradiated tomato plants are indicated by the red dashed line (<b>A</b>). The other fungal communities were found on the surface of premature enlarged green tomato fruits harvested from six air-irradiated tomato plants as a control (<b>A</b>). Each fungal communities at the phylum level is shown in panel B (<b>B</b>).</p>
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15 pages, 4783 KiB  
Review
Research Progress on the Characteristics of Nitrogen and Phosphorus Uptake by Ulva prolifera, the Dominant Macroalga Responsible for Green Tides in the Yellow Sea
by Yichao Tong, Yuqing Sun, Jing Xia and Jinlin Liu
Coasts 2025, 5(1), 10; https://doi.org/10.3390/coasts5010010 - 4 Mar 2025
Viewed by 46
Abstract
The abnormal proliferation of Ulva in the Yellow Sea has instigated the notorious green tide phenomenon. Mitigating this ecological challenge necessitates a holistic comprehension of Ulva’s nitrogen and phosphorus uptake behaviors. Investigating the mechanisms governing nutrient absorption, encompassing factors like concentration, form, [...] Read more.
The abnormal proliferation of Ulva in the Yellow Sea has instigated the notorious green tide phenomenon. Mitigating this ecological challenge necessitates a holistic comprehension of Ulva’s nitrogen and phosphorus uptake behaviors. Investigating the mechanisms governing nutrient absorption, encompassing factors like concentration, form, and input dynamics, has unveiled their profound influence on nutrient assimilation rates. The nutrient absorption characteristics of Ulva prolifera, including its preference for abundant nutrients, a high nitrogen-to-phosphorus (N/P) ratio, and its ability to efficiently absorb nutrients during pulse nutrient input events, determine its dominant role in the green tide events in the Yellow Sea. Although source control and preemptive salvaging are effective methods for managing green tides, addressing the root causes of these coastal ecological disasters requires the implementation of long-term pollution control strategies that align with sustainable development goals, with a priority on reducing marine eutrophication. This is crucial for the effective management and restoration of the coastal ecosystem in the Yellow Sea. Full article
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<p>(<b>a</b>) Photograph depicting the green tide event on 26 June 2021, with a maximum coverage area of 1746 km<sup>2</sup> in the Southern Yellow Sea [<a href="#B12-coasts-05-00010" class="html-bibr">12</a>]. (<b>b</b>) Field observation of floating <span class="html-italic">Ulva prolifera</span> in the coastal waters near Qingdao during the event. (<b>c</b>) The morphological characteristics of <span class="html-italic">U. prolifera</span> thalli.</p>
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<p>Schematic diagram of the discharge of nutrients into the Yellow Sea.</p>
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<p>Images depicting: (<b>a</b>) <span class="html-italic">Ulva prolifera</span> outbreak caused by the direct discharge of factory sewage into the sea in Jiangsu Province; (<b>b</b>) <span class="html-italic">Ulva prolifera</span> outbreaks in river estuaries in Jiangsu Province; (<b>c</b>) A benthic growth of <span class="html-italic">U. prolifera</span> caused by eutrophication in a pond; (<b>d</b>) Green tide outbreak affecting the Qingdao sea area in Shandong Province.</p>
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<p>(<b>a</b>) Areas affected by eutrophication in China from 2012 to 2020. The eutrophication level was determined by calculating the eutrophication index (E) as follows: E = [chemical oxygen demand] × [inorganic chlorine] × [labile phosphate] × 10<sup>6</sup>/4500. The eutrophic state was defined as E ≥ 1; moderate and severe eutrophication levels were defined as 3 &lt; E ≤ 9 and E &gt; 9, respectively. (Data sourced from the China Marine Ecological Environment Status Bulletin <a href="https://www.mee.gov.cn/hjzl/sthjzk/jagb/" target="_blank">https://www.mee.gov.cn/hjzl/sthjzk/jagb/</a>, accessed on 1 November 2024). (<b>b</b>) Simulated inputs of dissolved inorganic nitrogen (DIN), dissolved inorganic phosphorus (DIP), dissolved organic nitrogen (DON), and dissolved organic phosphorus (DOP) in the Yellow Sea in the past (1970, 2000) and future (2030, 2050). GO and AM indicate the Global Orchestration and Adapting Mosaic scenarios from the Millennium Ecosystem Assessment, respectively [<a href="#B61-coasts-05-00010" class="html-bibr">61</a>].</p>
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<p>Water quality distribution in the Yellow Sea, China, in 2020 [<a href="#B80-coasts-05-00010" class="html-bibr">80</a>].</p>
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20 pages, 10682 KiB  
Article
Temporal Profiling of Cellular and Molecular Processes in Osteodifferentiation of Dental Pulp Stem Cells
by Bibiána Baďurová, Kristina Nystøl, Terézia Okajček Michalič, Veronika Kucháriková, Dagmar Statelová, Slavomíra Nováková, Ján Strnádel, Erika Halašová and Henrieta Škovierová
Biology 2025, 14(3), 257; https://doi.org/10.3390/biology14030257 - 4 Mar 2025
Viewed by 39
Abstract
Based on the potential of DPSCs as the most promising candidates for bone tissue engineering, we comprehensively investigated the time-dependent cellular and molecular changes that occur during their osteodifferentiation. To analyze this area in-depth, we used both cellular and molecular approaches. Morphological changes [...] Read more.
Based on the potential of DPSCs as the most promising candidates for bone tissue engineering, we comprehensively investigated the time-dependent cellular and molecular changes that occur during their osteodifferentiation. To analyze this area in-depth, we used both cellular and molecular approaches. Morphological changes were monitored using bright-field microscopy, while the production of mineral deposits was quantified spectrophotometrically. The expression of a key mesenchymal stem cell marker, CD90, was assessed via flow cytometry. Finally, protein-level changes in whole cells were examined by fluorescence microscopy. Our results show successful long-term osteodifferentiation of the patient’s DPSCs within 25 days. In differentiated cells, mineralized extracellular matrix production gradually increased; in contrast, the expression of the specific stem cell marker CD90 significantly decreased. We observed dynamic changes in intracellular and extracellular proteins when collagen1 A1 and osteopontin appeared as earlier markers of osteogenesis, while apolipoprotein A2, bone morphogenetic protein 9, dentin sialophosphoprotein, and matrix metalloproteinase 8 were produced mainly in the late stages of this process. A decrease in actin microfilament expression indicated a reduction in cell proliferation, which could be used as another marker of osteogenic initiation. Our results suggest a coordinated process in vitro in which cells synthesize the necessary proteins and matrix components to regulate the growth of hydroxyapatite crystals and form the bone matrix. Full article
(This article belongs to the Special Issue Bone Cell Biology)
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<p>Cell morphology changes and cell density monitored by light microscopy before and during osteodifferentiation of DPSCs over 25 days. Control cells were cultivated in standard basal medium and differentiated cells in osteodifferentiation medium, which induced calcium deposit production in cells.</p>
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<p>Alizarin Red staining of calcium deposits of both control (DPSCs) and osteodifferentiated (Osteo) cells in a time-dependent manner over 25 days from the beginning of osteodifferentiation. (<b>a</b>) The stained mineral deposits were visualized by light microscopy; (<b>b</b>) Mineral deposit quantification was measured by a spectrophotometer. The graph shows an increase in calcium deposit production over 25 days. Gradual increase in calcium compounds in osteodifferentiated cells confirms bone matrix production. Control cells have a value of 100% at all time points. The values are the mean ± SD control and osteodifferentiated cells (<span class="html-italic">n</span> = 6). The statistical significance of the change between control and differentiated cells on each day is represented by the <span class="html-italic">p</span>-values <span class="html-italic">p</span> ≤ 0.05 (**) and <span class="html-italic">p</span> ≤ 0.005 (***).</p>
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<p>Flow cytometry analysis of control (DPSCs) and osteodifferentiated cells (Osteo) over 25 days. (<b>a</b>) Histograms show expression of CD90, a surface-specific marker of stem cell phenotype, in both types of cells; (<b>b</b>) The graph shows a decrease in CD90 expression in osteodifferentiated cells over a period of 25 days. Control cells have a value of 1 at all time points. The values are the mean ± SD control and osteodifferentiated cells (<span class="html-italic">n</span> = 6). The statistical significance of the change between control and differentiated cells on each day is represented by the <span class="html-italic">p</span>-values <span class="html-italic">p</span> ≤ 0.05 (**) and <span class="html-italic">p</span> ≤ 0.005 (***).</p>
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<p>Immunocytochemical staining of osteogenesis markers was performed using fluorescent antibodies over 25 days following the initiation of osteodifferentiation in dental pulp stem cells (DPSCs). Both control and osteodifferentiated cells were stained on the 5th, 10th, 15th, 20th, and 25th days with specific fluorescent antibodies against apolipoprotein A2 (APOA2), bone morphogenetic protein 9 (BMP9), collagen1 A1 (COL1A1), dentin sialophosphoprotein (DSPP), matrix metalloproteinase 8 (MMP8), and osteopontin (OPN). Actin filaments of the cytoskeleton were labeled with an antibody against phalloidin (PHALL). This figure represents the osteodifferentiated cells, while images of the control cells are shown in <a href="#app1-biology-14-00257" class="html-app">Figure S2</a>. Fluorescent signals were captured and analyzed using fluorescence microscopy.</p>
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<p>The graphs provide a graphical representation of the abundance of specific proteins, apolipoprotein A2 (APOA2), bone morphogenetic protein 9 (BMP9), collagen1 A1 (COL1A1), dentin sialophosphoprotein (DSPP), matrix metalloproteinase 8 (MMP8), osteopontin (OPN), and phalloidin (PHALL), which binds to actin filaments. The graphs represent the quantification of results from immunocytochemical analysis conducted over 25 days following the initiation of osteodifferentiation in DPSCs. The data are presented as a relative ratio to control cells, with control cells assigned a value of 1 (red line). The values are the mean ± SD osteodifferentiated vs. control cells (<span class="html-italic">n</span> = 6). The statistical significance of the change between control and differentiated cells on each day is represented by the <span class="html-italic">p</span>-values <span class="html-italic">p</span> ≤ 0.5 (*), <span class="html-italic">p</span> ≤ 0.05 (**), and <span class="html-italic">p</span> ≤ 0.005 (***).</p>
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