Search Results (690)

In-office teeth bleaching is an esthetic dental procedure performed to whiten teeth. A desensitizing procedure often requires prior in-office bleaching to relieve tooth hypersensitivity. The objective of this study was to evaluate the bleaching efficiency of teeth specimens pre-treated with different desensitizing agents by analyzing the color parameters. A total of 25 bovine specimens were stained and divided into five groups (n = 5). The specimens were pre-treated with GLUMA, ICON, bonding, or fluoride varnish according to the manufacturer’s instructions. Subsequently, the samples underwent three consecutive 20 min bleaching sessions using an in-office bleaching product. Color values in the CIE L*, a*, and b* color space were initially recorded, both before any treatment and after each bleaching session. The color difference (ΔE) was then computed. The data were analyzed using SPSS 24.0 software. The color alteration after pre-treatment was analyzed using a one-way ANOVA test, and the color alteration in each bleaching session was compared using a repeated-measure ANOVA test. A one-way ANOVA analysis showed a statistically significant difference in the bleaching efficiency of teeth specimens pre-treated with different desensitizers (p < 0.001). The repeated-measure ANOVA analysis showed no statistically significant difference in additional bleaching sessions (p = 0.133). The ICON and bonding pre-treatment showed the color alteration of teeth specimens before the bleaching procedure. Although the different desensitizer pre-treatments showed significant differences in bleaching efficiency, the differences in color parameters of the teeth specimens at the end of three bleaching sessions were statistically insignificant. Desensitizer pre-treatment influenced the degree of color change during multiple bleaching sessions; however, the outcome of bleaching was not affected by desensitizer pre-treatment. Full article

Megaproject construction endeavors and technological innovation activities, led by industry–university–research (IUR) collaboration, demonstrate marked disparities in value orientations, implementing entities, and constituent components. These discrepancies lead to a mismatch between innovation demands and actual activities, as well as insufficient innovation motivation among construction entities, subsequently impacting innovation effectiveness and the commercialization of outcomes and failing to adequately support engineering construction needs. In response to this predicament, the academic community widely acknowledges IUR collaborative innovation as a solution. This research integrates fundamental theoretical analysis with a multi-case study approach and systematically dissects the distinctive features at the micro, meso, and macro levels, grounded in the collaborative innovation practices of IUR in three iconic railway engineering projects in China. Subsequently, it unravels the inherent operational mechanics of the IUR collaborative innovation system within large-scale projects. Specifically, at the micro level, the profound engagement of governments and project owners fosters a solid supportive environment and collaborative platform for IUR collaboration, while past successful cooperation experiences among key innovation entities enhance their technological and knowledge interactions. At the meso level, shared industry cognitions and values, hierarchical organizational structures, flexible institutional designs, and resource allocation strategies based on balancing risks and benefits collectively constitute the supporting system for megaproject collaborative innovation. At the macro level, the tight integration of the innovation chain and industrial chain promotes the formation of an open cooperation ecosystem, ensuring the continuity and systematic nature of innovation activities and accelerating the rapid commercialization and efficient utilization of innovation outcomes. This study not only enriches the theoretical connotations of IUR collaborative innovation in the context of major engineering projects but also provides theoretical foundations for strategy formulation and management practices for major project managers, holding significant value in guiding the innovation management of future major engineering projects. Full article

Background/Objectives: Animals destined for human consumption play a key role in potentially transmitting bacteria carrying antibiotic resistance genes. However, there is limited knowledge about the carriage of antibiotic-resistant bacteria in native breeds. We aimed to characterize the phenotypic profiles and antibiotic resistance genes in Escherichia coli isolated from bovines, including three native Portuguese bovine breeds. Methods: Forty-nine E. coli isolates were selected from 640 fecal samples pooled by age group (eight adult or eight calf samples) from each farm, representing both dairy cattle raised in intensive systems and meat cattle raised in extensive systems in Northern Portugal. The presumptive E. coli colonies plated onto MacConkey agar were confirmed using matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). The antibiotic resistance profiles were screened by antimicrobial susceptibility testing (EUCAST/CLSI guidelines), and the antibiotic resistance genes by PCR. Results: Most isolates showed resistance to ampicillin (69%), tetracycline (57%), gentamicin (55%), and trimethoprim + sulfamethoxazole (53%), with no resistance to imipenem. Resistance to at least one antibiotic was found in 92% of isolates, while 59% exhibited multidrug resistance. Most calf isolates, including those from native breeds, showed a multidrug-resistant phenotype. Among the adults, this was only observed in Holstein-Friesian and Barrosã cattle. None of the Holstein-Friesian isolates were susceptible to all the tested antibiotics. ESBL-producing E. coli was identified in 39% of isolates, including those from Holstein-Friesian calves and adults, Cachena calves and Minhota adults. The sul2 gene was detected in 69% of isolates, followed by bla_CTX-M (45%), aac(3′)-IV (41%), and aac(6′)-Ib-cr (31%), with a higher prevalence in adults. Conclusions: This pioneering study highlights the concerning presence of multidrug-resistant E. coli in native Portuguese cattle breeds. Full article

Over the past several years, many iconic national parks in the US have experienced large increases in visitation. At the same time, lesser-known units have had declining numbers. Both issues challenge the sustainable management of parks. The purpose of this study is to use motivation theory to develop and test the differences between market segments at a western US national historic site. The ultimate goal is to contribute to sustainable visitation management by increasing visitation and enhancing the visitor experience. A market segmentation analysis can identify and describe promising target markets. The data were collected using an onsite exit survey, resulting in a sample of 570 visitors over the high season. Three clusters of visitors were identified: heritage immersers, history appreciators, and casual sightseers. The groups differed significantly with respect to visitation motives, travel, and visitation patterns. Segments are described and implications offered. The study confirms differences among cultural heritage visitors, with some being promising as target markets for the historic site. The study underscores the importance of tailoring site management and marketing strategies to meet the diverse needs of visitors and supports the use of motive-based segmentation as a means of better understanding and sustainably managing visitor experiences. Full article

This study aimed to evaluate the change in enamel color and surface micro-hardness following the use of resin-infiltration concept material (ICON) and casein phosphopeptide-amorphous calcium fluoride phosphate (CPP-ACFP) remineralizing agent. Fifty-four extracted human third molars were collected and randomly divided into three groups: group A: control with no surface treatment; group B: treated using ICON; and group C: treated using CPP-ACFP. The change in color and micro-hardness of the enamel surface were measured using spectrophotometer and Vickers hardness number, respectively. The measurements were taken at three timelines; baseline (BL), after demineralization (DM), and after surface treatment (TX). The three groups showed no significant differences in enamel color change after demineralization (p < 0.05). However, after surface treatment in relation to the baseline, groups B and C had a significant increase in color change compared to the control group (p < 0.05), and group B showed a statistically significant increase in enamel color changes compared to group C. Additionally, all groups exhibited a significant reduction in enamel micro-hardness after demineralization in comparison to their baseline (p < 0.05). Group C showed a significant increase in micro-hardness after surface treatment compared to groups A and B (p < 0.05), while group B showed a significant decrease in enamel micro-hardness compared to groups A and C (p < 0.05). These findings suggest that teeth treated with CPP-amorphous calcium fluoride phosphate (CPP-ACFP) show a significant improvement in enamel surface color after demineralization compared to the teeth treated with resin infiltration (ICON) and the non-treated teeth. Additionally, enamel surfaces treated with CPP-ACFP show significant enamel hardness regaining, while resin infiltration (ICON) compromises enamel surface hardness. Full article

In the post-COVID-19 era, there has been an increasing interest in re-evaluating citizens’ living conditions within dense and grey urban areas. The provision of green spaces has always been identified as an important aspect of alleviating contemporary everyday life stress and preventing or limiting mental health-related issues. It is also an important strategy to mitigate urban heat islands and foster adaptation strategies to climate change. Among the numerous experiments of ‘green action’ available to urban planners, urban farming strategies have been widely used in Europe to provide green spaces and ecosystem services, exploring the topics related to self-production of food, biodiversity, and zero-km cultivation. Therefore, finding new spaces for agriculture in urban environments has driven scientists, researchers, and entrepreneurs to develop new soilless technologies (such as hydroponics, aquaponics, and aeroponics) to maximize yields in urban areas, creating new agricultural and architectural models such as the vertical farms (VF) and the building-integrated greenhouses (BIGH). In this regard, the objective of this paper is to recontextualize the integrated greenhouse element for high-tech food production as new iconic architectural models derived from the experience of the Victorian Winter Gardens and the first tropical greenhouses. Revisiting these perspectives, this paper offers opportunities to redefine the greenhouse as a multifunctional asset that aligns with both environmental goals and architectural standards. Full article

The reduction of leaves was a key event in the evolution of the succulent syndrome in Cactaceae, evolving from large, photosynthetic leaves in Pereskia to nearly suppressed microscopic foliar buds in succulent Cactoideae. This leaf reduction was accompanied by the development of spines. Early histological studies, dating back a century, of the shoot apical meristem (SAM) in several species concluded that, in succulent cacti, axillary buds became areoles and leaves transformed into spines. However, these conclusions were based on limited observations, given the challenges of obtaining SAM samples from long-lived, often endangered species. Here, we present a complete study of early aerial organ development in seedlings of the iconic Carnegiea gigantea (saguaro), characterizing the different stages of seedling development. We focus on the SAM to track the emergence and development of primordia and aerial organs, closely following the spine development from undifferentiated structures. We demonstrate that young, few-days-old saguaro seedlings provide a valuable model for morpho-anatomical and molecular studies in Cactaceae. We also outline optimal laboratory practices for germinating saguaro seeds and conducting histological studies. Our observations confirm the absence of clear foliar structures and the presence of a distinct type of primordia, hypothesized to be foliar but lacking definitive foliar features. Based on our observations and a review of the literature, we revive the discussion on the ontogenetic origin of spines and propose saguaro seedlings as a promising model for studying the genetic identity of SAM primordia. Full article

We demonstrate that a short oligonucleotide complementary to a G-quadruplex domain can invade this iconic, noncanonical DNA secondary structure in ways that profoundly influence the properties and differential occupancies of the resulting DNA polymorphic products. Our spectroscopic mapping of the conformational space of the associated reactants and products, both before and after strand invasion, yield unanticipated outcomes which reveal several overarching features. First, strand invasion induces the disruption of DNA secondary structural elements in both the invading strand (which can assume an iDNA tetrad structure) and the invaded species (a G-quadruplex). The resultant cascade of coupled alterations represents a potential pathway for the controlled unfolding of kinetically trapped DNA states, a feature that may be characteristic of biological regulatory mechanisms. Furthermore, the addition of selectively designed, exogenous invading oligonucleotides can enable the manipulation of noncanonical DNA conformations for biomedical applications. Secondly, our results highlight the importance of metastability, including the interplay between slower and faster kinetic processes in determining preferentially populated DNA states. Collectively, our data reveal the importance of sample history in defining state populations, which, in turn, determine preferred pathways for further folding steps, irrespective of the position of the thermodynamic equilibrium. Finally, our spectroscopic data reveal the impact of topological constraints on the differential stabilities of base-paired domains. We discuss how our collective observations yield insights into the coupled and uncoupled cascade of strand-invasion-induced transformations between noncanonical DNA forms, potentially as components of molecular wiring diagrams that regulate biological processes. Full article

The interior of Orthodox churches is entirely decorated with paintings, icons, and frescoes, to help create a special environment for the prayers and visitors. The paintings have religious, esthetic, and historical value, being created in the Byzantine tradition and following recommendations on style and materials according to old church canons. Particular importance is attributed to the Iconostasis decorated with icons on several registers, as well as to polychrome wooden ornaments and imperial doors. This work presents the scientific investigation results of three royal icons, theoretically dating to the 19th century, from the Iconostasis of the Runcu church, an important 19th-century Romanian historical monument, aiming to answer questions regarding their age and constituent materials, especially what were the mineral pigments used in the color palettes for their painting and if there were any old repainting interventions, in order to assist their restoration and conservation. Methods of characterization employed were optical microscopy, SEM-EDX spectrometry, ATR/FTIR spectroscopy, and X-ray fluorescence spectrometry. Results obtained allowed us to date, attribute, and evaluate the conservation state of the Royal icons; to our knowledge, this study is among the very few research studies carried out so far on the religious heritage conservation field in Romania. Full article

Following the Great East Japan Earthquake, dark tourism was developed in the Tohoku Region of Japan. Notably, two government-built tsunami memorial parks in Ishinomaki and Rikuzentakata have obtained attention for their profound disaster narratives, iconic disaster sites, and expansive layouts. This study is the first to compare the projected destination image presented by destination management organizations with the perceived destination image held by visitors in these parks, and in dark tourism. Using online text data from both supply and demand sides of dark tourism and text mining analyses such as word frequency analysis, co-occurrence network analysis, and affection tendency examination, we revealed similarities and disparities between these two perspectives. Furthermore, this study concluded dimensions specific to dark tourism sites within the cognitive and affective destination image. Based on the findings, the study provides advice for destination managers to improve these sites, including developing non-dark tourism products and improving infrastructures. Additionally, it proposes placing greater emphasis on themes of revitalization and future development, while fostering visitor engagement in local non-profit and citizen activities to strengthen connections with residents. The findings demonstrate the effectiveness of text mining in comparing projected and perceived destination images in the context of dark tourism sites. Full article

The complex phylogenetic relationship of polyploid species provides an opportunity for a comprehensive study of gene introgression. Oil camellias refer to a class of important woody oil plant in the camellia genus, including octoploid, hexaploid, tetraploid, and diploid plants, but the phylogeny relationship of these species remains poorly investigated. Here, based on multiple types of evidence, including phylogenetic conflict, gene flow analysis, and representative metabolite, we reconstructed the phylogenetic relationship of oil camellias. Camellia shensiensis and C. grijsii formed a distinct branch. Phylogenetic conflict suggested that hexaploid C. oleifera probably originated from hybridization and clustered with diploid C. kissi and tetraploid C. meiocarpa. Tetraploid C. confusa probably originated from crossing the ancestor of C. kissi and C. brevistyla, and C. brevistyla probably was the maternal progenitor of hexaploid C. sasanqua. Furthermore, the composition of anthocyanin in tender leaves showed a strong correlation with phylogenetic distinctions. This study proves the feasibility of using iconic metabolic components to solve phylogenetic relationships and lays a foundation for analyzing genetic breeding and utilizing oil camellia resources. Full article

Gut commensals play important roles in maintaining the homeostasis of human health. Previous studies indicated that the abundance of P. goldsteinii in animal hosts was increased by the administration of prebiotics such as polysaccharides purified from iconic oriental medicinal fungi. Subsequently, P. goldsteinii was found to exert beneficial effects on the amelioration of multiple chronic inflammation-associated diseases. Even so, during the process of the development of P. goldsteinii as a next-generation probiotic (NGP), care has to be taken when it is used as a functional food ingredient. In this study, we isolated a novel P. goldsteinii strain, RV-01, from the feces of a healthy adult and carried out comprehensive analyses of its genomic and phenotypic characteristics. Bioinformatic analysis of P. goldsteinii RV-01 revealed the absence of potential virulence genes, as well as the presence of genes and traits potentially beneficial to human health, such as the production of short-chain fatty acids, anti-inflammatory lipopolysaccharides, and zwitterionic capsular polysaccharides, as well as immune regulatory proteins. To circumvent any potential side effects, the P. goldsteinii RV-01 was autoclaved before proceeding to the nonclinical safety assessment. The autoclaved P. goldsteinii RV-01 retained its anti-inflammatory effect in human colon epithelial cells. In addition to the three genotoxicity assays, 28-day subacute and 90-day subchronic animal toxicity studies (the highest dose tested was equivalent to 8.109 × 10¹⁰P. goldsteinii RV-01 cells/kg body weight/day) were also implemented. The results of all studies were negative for toxicity. These results support the conclusion that autoclaved P. goldsteinii RV-01 is safe for use as a food ingredient. Full article

Background/Objectives: Front-of-package labeling (FOPL) policies are a useful strategy to inform consumers about foods high in nutrients of concern, but little is known about what type of label works best in Bangladesh, a country with increasing levels of unhealthy food intake and diet-related diseases. Methods: We conducted 10 focus groups with men and women in rural and urban Bangladesh (n = 76). Using a semi-structured discussion guide, we asked consumers for their perceptions of the healthfulness of nutrients and foods, two common FOPLs (a color-coded guideline daily allowance [GDA] label and a warning label), and different visual elements of the warning label (e.g., shape, icon, text). Results: Participants understood the health harms of sugar and salt consumption but were less clear on saturated fat. Both FOPLs were perceived as helpful for identifying unhealthy foods, but the warning labels were perceived as easier to understand and more likely to influence behaviors than the GDA. Regarding the design of warning labels, participants perceived warning devices, holding straps, and octagonal shapes as effective but had mixed reactions to which icons or textual statements were most effective. Conclusions: FOPLs are likely to facilitate Bangladeshi consumers’ ability to identify unhealthy products. Further research is needed to understand the impact on food choices as well as the most effective design in this population. Full article

“Bluebeard” (ATU 321: Maiden-Killer), a fairy tale about a wealthy noble man and serial killer, is the most gruesome of Charles Perrault’s fairy tales. Bluebeard epitomizes evil and horror. In Perrault’s tale, Bluebeard’s evilness is linked to patriarchy and power, as symbolized by the villain’s iconic blue beard. Historically linked to Henry VIII (1491–1547), King of England, Bluebeard has also been associated with Breton commander Gilles de Rais who was hanged for sorcery and satanic abuse. This article examines how contemporary francophone “Bluebeard” variants refashion and redefine evil and whether they contain any new morals linked to evilness. Do they depict Bluebeard as a satanic, intrinsic force of evil or do they portray him in a less Manichean manner, as contemporary tales tend to do with monsters? Starting with Perrault’s famous tale, this article reveals how Bluebeard, the evil mass murderer figure and polygamist, is recast in a variety of contemporary francophone texts from Morocco, Belgium and France, with retellings by Michel Tournier (1981), Marie Darrieussecq (2002), La Barbe Bleue (Bluebeard) (2009), Amélie Nothomb (2012), Tahar Ben Jelloun (2014), Jacqueline Kelen (2014), and Cécile Coulon (2015). These modern variants illustrate Elliott Oring’s ideas about comparison and cultural context (see Oring 1986). A discussion of various French contemporary versions with a special emphasis of Ben Jelloun’s Moroccan retelling of “Bluebeard” open avenues for cross-cultural dialogue, highlighting how this tale evolves to fit different cultural contexts and continues to resonate today. Full article

With the rapid development of autonomous driving technology, ensuring the safety and reliability of vehicles under various complex and adverse conditions has become increasingly important. Although autonomous driving algorithms perform well in regular driving scenarios, they still face significant challenges when dealing with adverse weather conditions, unpredictable traffic rule violations (such as jaywalking and aggressive lane changes), inadequate blind spot monitoring, and emergency handling. This review aims to comprehensively analyze these critical issues, systematically review current research progress and solutions, and propose further optimization suggestions. By deeply analyzing the logic of autonomous driving algorithms in these complex situations, we hope to provide strong support for enhancing the safety and reliability of autonomous driving technology. Additionally, we will comprehensively analyze the limitations of existing driving technologies and compare Advanced Driver Assistance Systems (ADASs) with Full Self-Driving (FSD) to gain a thorough understanding of the current state and future development directions of autonomous driving technology. Full article