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Search Results (371)

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18 pages, 607 KiB  
Article
Amnat Charoen Healers in Thailand and Their Medicinal Plants
by Auemporn Junsongduang, Surapon Saensouk and Henrik Balslev
Plants 2025, 14(4), 602; https://doi.org/10.3390/plants14040602 - 17 Feb 2025
Abstract
Medicinal plants remain vital in the Phu Tai community in Amnat Charoen in Thailand. Traditional healers’ knowledge is largely undocumented in the literature. Our objective was to document their medicinal plant practices to preserve this valuable knowledge. Our informants were 15 Phu Tai [...] Read more.
Medicinal plants remain vital in the Phu Tai community in Amnat Charoen in Thailand. Traditional healers’ knowledge is largely undocumented in the literature. Our objective was to document their medicinal plant practices to preserve this valuable knowledge. Our informants were 15 Phu Tai healers. We calculated use values (UV), family importance values (FIV), and informant agreement ratios (IAR) to gauge the significance of the 211 medicinal plants used by the healers. The most important plant families were Fabaceae and Zingiberaceae (FIV = 93). Kha min (Curcuma longa) was the most important medicinal species (UV = 0.66). The decoction was the most common preparation method (85%). Skin/subcutaneous cellular tissue disorders had the highest informant agreement ratio (IAR = 0.73). Shrubs were the most common life form (36%) among the medicinal plants; the majority were collected from community forests (51%) and were native to Thailand (86%). The most frequently used plant part for medicine was the leaf (27%). Medicinal plants that can be purchased were Ueang mai na (Hellenia speciosa), Thep tharo (Cinnamomum parthenoxylon), and Som khon (Talinum paniculatum). Interestingly, monks served as traditional healers. The healer’s age and education were not correlated with the number of medicinal plants they knew. The Amnat Charoen healers possess a rich traditional knowledge of medicinal plants. The information reported here is invaluable for further research in the field of cross-cultural ethnobotany and ethnopharmacology. Full article
(This article belongs to the Special Issue Genetic Resources and Ethnobotany in Aromatic and Medicinal Plants)
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<p>Location of Amnat Charoen province in Thailand where ethnobotanical data concerning medicinal plants were collected in Chanuman district.</p>
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14 pages, 596 KiB  
Review
Lords-and-Ladies (Arum) as Food in Eurasia: A Review
by Łukasz Łuczaj and Gizem Emre
Plants 2025, 14(4), 577; https://doi.org/10.3390/plants14040577 - 13 Feb 2025
Abstract
(1) Background. Although Arum spp. are toxic in their raw state, they are sometimes used as food within their native ranges. (2) Methods. We review the available literature in order to provide an overview of its use and detoxification procedures worldwide. (3) Results. [...] Read more.
(1) Background. Although Arum spp. are toxic in their raw state, they are sometimes used as food within their native ranges. (2) Methods. We review the available literature in order to provide an overview of its use and detoxification procedures worldwide. (3) Results. The food use of lords-and-ladies was already mentioned by Theophrastus, Dioscorides, Matthioli, Durante, Gerard, and Sirennius. In the references concerning 19th–21st-century use, seven species were identified: A. cyrenaicum, A. discoridis, A. italicum, A. maculatum, A. orientale, A. palaestinum, and A. rupicola. Past or current culinary use of the plant has been recorded in Morocco, Libya, the United Kingdom, the Scilly Islands, Germany, Switzerland, Italy, Romania, Ukraine (including Crimea), Czechia, Slovenia, Croatia, Bosnia-Herzegovina, Albania, Georgia, Türkiye, Syria, Palestine, Lebanon, Israel, Iraq, and Iran. (4) In Europe, rhizomes were used, mainly as a famine food. In SW Asia, the aerial parts remain an important element of local cuisine. Several detoxification procedures are used before consumption, such as prolonged boiling, often involving straining the boiled water and lowering the pH with lemon juice, sumac, citric acid, sorrel leaves, or pomegranate juice. (5) Conclusions. Further studies are needed to assess the safety of Arum use and record traditional local recipes in SW Asia. Full article
(This article belongs to the Special Issue Ethnobotany and Botany in the Euro-Mediterranean Region)
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<p>Distribution of the genus <span class="html-italic">Arum</span> in the world. Green—native range, purple—non-native range [<a href="#B9-plants-14-00577" class="html-bibr">9</a>].</p>
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54 pages, 4287 KiB  
Review
Ethnobotany, Phytochemistry, and Biological Activity of Extracts and Non-Volatile Compounds from Lantana camara L. and Semisynthetic Derivatives—An Updated Review
by Jorge Ramírez, Chabaco Armijos, Nelson Espinosa-Ortega, Leydy Nathaly Castillo and Giovanni Vidari
Molecules 2025, 30(4), 851; https://doi.org/10.3390/molecules30040851 - 12 Feb 2025
Abstract
Lantana camara L., commonly known as pigeon berry, is a herbaceous plant of growing scientific interest due to the high medicinal value. In fact, despite being categorized as an invasive species, it has been used for a long time to treat different diseases [...] Read more.
Lantana camara L., commonly known as pigeon berry, is a herbaceous plant of growing scientific interest due to the high medicinal value. In fact, despite being categorized as an invasive species, it has been used for a long time to treat different diseases thanks to the many biological activities. Triterpenes, flavonoids, phenylpropanoids, and iridoid glycosides are the bioactive compounds naturally occurring in L. camara that have demonstrated anticancer, antifilarial, nematocidal, antibacterial, insecticidal, antileishmanial, antifungal, anti-inflammatory, and antioxidant properties. The aim of this review is to update the information concerning the chemistry and biological activity of L. camara extracts and their constituents, including semisynthetic derivatives, revising the literature until June 2024. We believe that the data reported in this review clearly demonstrate the importance of the plant as a promising source of medicines and will therefore stimulate further investigations. Full article
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Graphical abstract

Graphical abstract
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<p><span class="html-italic">Lantana camara</span> L.: (<b>A</b>) entire plant; (<b>B</b>) flowers; (<b>C</b>) fruits (photos by the authors).</p>
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<p>Worldwide distribution of <span class="html-italic">Lantana camara</span> L. [<a href="#B11-molecules-30-00851" class="html-bibr">11</a>].</p>
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<p>Flowchart for the search process and selection of the studies considered for the review.</p>
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<p>Structures of compounds <b>3</b>, and <b>6</b>–<b>15</b>.</p>
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<p>Structures of compounds <b>16</b>, <b>17</b>, and <b>19</b>–<b>30</b>.</p>
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<p>Structures of compounds <b>31</b>–<b>47</b>.</p>
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<p>Structures of compounds <b>48</b>–<b>74</b>.</p>
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<p>Structures of compounds <b>75</b>–<b>90</b>.</p>
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<p>Structures of compounds <b>91</b>–<b>96</b>.</p>
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<p>Structures of compounds <b>97–107</b> and <b>109</b>–<b>120</b>.</p>
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<p>Structures of compounds <b>121</b>–<b>127</b>.</p>
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<p>Structures of compounds <b>128</b>–<b>138</b>.</p>
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<p>Structures of compounds <b>139</b>–<b>151</b>.</p>
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<p>Structures of compounds <b>152</b>, <b>153</b>, and <b>159</b>–<b>168</b>.</p>
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23 pages, 2667 KiB  
Article
Phylogenetic Perspectives and Ethnobotanical Insights on Wild Edible Plants of the Mediterranean, Middle East, and North Africa
by Mousaab Alrhmoun, Naji Sulaiman and Andrea Pieroni
Foods 2025, 14(3), 465; https://doi.org/10.3390/foods14030465 - 1 Feb 2025
Abstract
This study investigates the phylogenetic and geographical distribution of wild food plants (WFPs) across 30 Mediterranean and North African (MENA) regions, focusing on the intersection of evolutionary lineage, ecological adaptation, and cultural utilization. A phylogenetic analysis of 111 genera of WFPs used in [...] Read more.
This study investigates the phylogenetic and geographical distribution of wild food plants (WFPs) across 30 Mediterranean and North African (MENA) regions, focusing on the intersection of evolutionary lineage, ecological adaptation, and cultural utilization. A phylogenetic analysis of 111 genera of WFPs used in traditional diets reveals clusters reflecting shared ancestry, functional adaptations, and ecological resilience. Key regions such as Lebanon and Ikaria stand out as potential centers for the diversity of wild food plant use, suggesting that the Eastern Mediterranean may be a primary origin area, especially for species adapted to semi-arid climates. Major plant families including Lamiaceae, Rosaceae, and Fabaceae form distinct clusters that underscore their common ancestry and adaptability, making them foundational to traditional diets and medicinal applications across various environments. Geographical analysis indicates historical connections, such as those between Malta and Egypt, supporting the hypothesis that ancient trade routes influenced the spread and cultural exchange of wild food plant use across the Mediterranean. The study emphasizes the integration of phylogenetic and ethnobotanical perspectives, shedding light on how biodiversity, ecological adaptation, and cultural practices intersect in these regions. This research demonstrates that WFPs serve as both ecological and cultural assets, crucial for preserving traditional diets and supporting biodiversity conservation amid environmental changes. Integrating evolutionary and cultural knowledge can enrich ecological understanding and contribute to the sustainable use of plant resources in the MENA regions. Full article
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<p>The MENA (Mediterranean and North Africa) field study areas used in the comparative analysis (Central Ikaria (IKA), Central Crete (CRE), Lebanon (LEB), Tartus (SYR), Sidi Bouzid (TUN), Sicily (SIC), Central Armenia (ARM), Northern West Bank (PAL), Syrian–Turkish borderland (ASS), Kurdistan region of Iraq (KUR), Andalusia (ANSPA), Sanabria (SANSPA), Caurel (CAUSPA), Pilona (PILSPA), Picos de Europa (PDESPA), Campoo (CAMSPA), Montesinho (MONPOR), Catalonia (CASPA), Corsica (FRA), Alentejo (ALEPOR), Cyprus (CYP), Malta (MAL), Rif Mountains (MOR), Nile Delta (NDEGY), Siwa Oasis (SOEGY), Jordan Valley (JOR), Cyrenaica (LIB), Albanian South-East (ALB), Oman Mountains (OMA), and Western Algeria (ALG)).</p>
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<p>Geographic distribution of WFPs in the MENA regions documented in the study area. In Southern Europe, we identified Sicily (SIC), Andalusia (ANSPA), Sanabria (SANSPA), Caurel (CAUSPA), Pilona (PILSPA), Picos de Europa (PDESPA), Campoo (CAMSPA), Montesinho (MONPOR), Catalonia (CASPA), and Corsica (FRA). Western Europe is represented by Alentejo (ALEPOR). The Eastern Mediterranean includes Central Ikaria (IKA), Central Crete (CRE), and Cyprus (CYP), while Southeast Europe covers Central Armenia (ARM) and the Albanian South-East (ALB). In the Central Mediterranean, Malta (MAL) was identified as a key site. North Africa comprises Sidi Bouzid (TUN), the Rif Mountains (MOR), Western Algeria (ALG), Cyrenaica (LIB), the Nile Delta (NDEGY), and the Siwa Oasis (SOEGY). The Western Asia region includes Lebanon (LEB), Tartus (SYR), the Syrian–Turkish borderland (ASS), the Jordan Valley (JOR), and the Kurdistan region (KUR). Finally, the Arabian Peninsula is represented by the Oman Mountains (OMA).</p>
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<p>Frequency of wild food plant (genera) uses across study sites (Central Ikaria (IKA), Central Crete (CRE), Lebanon (LEB), Tartus (SYR), Sidi Bouzid (TUN), Sicily (SIC), Central Armenia (ARM), Northern West Bank (PAL), Syrian–Turkish borderland (ASS), Kurdistan region (KUR), Andalusia (ANSPA), Sanabria (SANSPA), Caurel (CAUSPA), Pilona (PILSPA), Picos de Europa (PDESPA), Campoo (CAMSPA), Montesinho (MONPOR), Catalonia (CASPA), Corsica (FRA), Alentejo (ALEPOR), Cyprus (CYP), Malta (MAL), Rif Mountains (MOR), Nile Delta (NDEGY), Siwa Oasis (SOEGY), Jordan Valley (JOR), Cyrenaica (LIB), Albanian South-East (ALB), Oman Mountains (OMA), and Western Algeria (ALG)).</p>
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<p>Dendrogram of wild plant distribution clusters among study sites (Central Ikaria (IKA), Central Crete (CRE), Lebanon (LEB), Tartus (SYR), Sidi Bouzid (TUN), Sicily (SIC), Central Armenia (ARM), Northern West Bank (PAL), Syrian–Turkish borderland (ASS), Kurdistan region (KUR), Andalusia (ANSPA), Sanabria (SANSPA), Caurel (CAUSPA), Pilona (PILSPA), Picos de Europa (PDESPA), Campoo (CAMSPA), Montesinho (MONPOR), Catalonia (CASPA), Corsica (FRA), Alentejo (ALEPOR), Cyprus (CYP), Malta (MAL), Rif Mountains (MOR), Nile Delta (NDEGY), Siwa Oasis (SOEGY), Jordan Valley (JOR), Cyrenaica (LIB), Albanian South-East (ALB), Oman Mountains (OMA), and Western Algeria (ALG)).</p>
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<p>The dendrogram groups the WFP genera across the studied regions. This analysis aimed to capture the most frequently cited and widely used WFPs among different sites, providing insight into common taxa and usage patterns.</p>
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<p>Phylogenetic relationships of WFPs and regional diversity across MENA sites: the phylogenetic clustering of WFPs and regional sites based on the wild plant diversity they harbor. (<b>A</b>) Panel represents the phylogenetic relationships among 111 wild plant species, where closely related taxa form distinct clusters, color-coded by divergence levels. (<b>B</b>) Panel illustrates the clustering of 30 sites from the MENA regions, based on the wild plant taxa they contain. (Central Ikaria (IKA), Central Crete (CRE), Lebanon (LEB), Tartus (SYR), Sidi Bouzid (TUN), Sicily (SIC), Central Armenia (ARM), Northern West Bank (PAL), Syrian–Turkish borderland (ASS), Kurdistan region (KUR), Andalusia (ANSPA), Sanabria (SANSPA), Caurel (CAUSPA), Pilona (PILSPA), Picos de Europa (PDESPA), Campoo (CAMSPA), Montesinho (MONPOR), Catalonia (CASPA), Corsica (FRA), Alentejo (ALEPOR), Cyprus (CYP), Malta (MAL), Rif Mountains (MOR), Nile Delta (NDEGY), Siwa Oasis (SOEGY), Jordan Valley (JOR), Cyrenaica (LIB), Albanian South-East (ALB), Oman Mountains (OMA), and Western Algeria (ALG)).</p>
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29 pages, 5400 KiB  
Article
Shifting Herbal Knowledge: The Ecological and Cultural Dynamics Behind Plant Use Changes in the Southern Occitan Alps
by Mousaab Alrhmoun, Naji Sulaiman and Andrea Pieroni
Plants 2025, 14(3), 367; https://doi.org/10.3390/plants14030367 - 25 Jan 2025
Viewed by 189
Abstract
This study examines changes in medicinal and wild food plant knowledge in the Alpine Southern Occitan area, focusing on temporal and cultural shifts. Drawing from ethnobotanical data from the Maira Valley (1970, 2022), Stura Valley (2004), and Grana Valley (2011), we explore dynamics [...] Read more.
This study examines changes in medicinal and wild food plant knowledge in the Alpine Southern Occitan area, focusing on temporal and cultural shifts. Drawing from ethnobotanical data from the Maira Valley (1970, 2022), Stura Valley (2004), and Grana Valley (2011), we explore dynamics in plant use and how they may have been generated by ecological, socio-economic, and cultural changes. A total of 308 plant taxa were recorded, with notable declines in Asteraceae and Lamiaceae utilizations by 2022, suggesting a remarkable erosion of traditional ecological knowledge (TEK). Conversely, the use of families like Brassicaceae and Amaranthaceae increased, possibly due to socio-economic factors. This study also found in recent years a decrease in medicinal plant uses and a greater reliance on food system-related plants. Logistic regression models highlighted altitude (600–1600 masl) as a key factor in plant diversity use, with older participants showing more diverse ethnobotanical knowledge. This study underscores the resilience and transformation of plant knowledge in response to socio-economic and environmental changes in the Alpine area. Full article
(This article belongs to the Section Phytochemistry)
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<p>Number of reported botanical plants in the past and present studies in the data area [<a href="#B22-plants-14-00367" class="html-bibr">22</a>,<a href="#B23-plants-14-00367" class="html-bibr">23</a>].</p>
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<p>Genera overlapping within the present data and previous studies conducted in the region from 1970 to 2022 [<a href="#B22-plants-14-00367" class="html-bibr">22</a>,<a href="#B23-plants-14-00367" class="html-bibr">23</a>].</p>
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<p>The distribution of plant part usage ((F) food, (FM) both food and medicinal, and (M) medicinal) across the studies ([<a href="#B22-plants-14-00367" class="html-bibr">22</a>,<a href="#B23-plants-14-00367" class="html-bibr">23</a>]), and our collected data in 2011, and 2022.</p>
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<p>(<b>A</b>) The interconnectedness of plant studies and the overlap in plant parts examined across different studies [<a href="#B22-plants-14-00367" class="html-bibr">22</a>,<a href="#B23-plants-14-00367" class="html-bibr">23</a>]. (<b>B</b>) The chord diagram confirms the dynamic interplay between all the studies over time.</p>
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<p>The chord diagram between all the usage and used parts in each data over time: (<b>A</b>) Rovera et al. [<a href="#B22-plants-14-00367" class="html-bibr">22</a>], (<b>B</b>) Musset and Dore [<a href="#B23-plants-14-00367" class="html-bibr">23</a>], (<b>C</b>) our collected data (2011), and (<b>D</b>) data (2022).</p>
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<p>The heatmap illustrates the Jaccard Index, measuring the similarity between plant species compositions across various families. Values range from 0 (no overlap) to 1 (complete similarity), represented by a gradient from dark blue (low similarity) to cyan (high similarity). The hierarchical clustering on both axes highlights groups of plant families with shared characteristics, as shown by closely aligned branches in the dendrogram.</p>
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<p>A dendrogram is a tree-like diagram used to visualize hierarchical relationships between data studies labeled (<b>A</b>) Rovera et al. [<a href="#B22-plants-14-00367" class="html-bibr">22</a>], (<b>B</b>) Musset and Dore [<a href="#B23-plants-14-00367" class="html-bibr">23</a>], (<b>C</b>) our data (2011), and (<b>D</b>) our data (2022).</p>
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<p>The biplot visualizes the relationships between the four studies based on their contribution to the principal components. The studies can be clustered into two groups: Cluster 1 comprising Rovera et al. [<a href="#B22-plants-14-00367" class="html-bibr">22</a>] and Musset and Dore [<a href="#B23-plants-14-00367" class="html-bibr">23</a>], and Cluster 2 including Data 2011 and Data 2022.</p>
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<p>Linguistic map of Piedmont (NW Italy) and the three considered Alpine Southern Occitan valleys (Maira, Stura, and Grana), nestled in the borderlands of Italy and France.</p>
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<p>Natural landscape in the Valle Grana (<b>A</b>) and Maria (<b>B</b>) (Photo: Site Maira Valley, Italy).</p>
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26 pages, 461 KiB  
Article
Persistence of Use Among Amazigh People of Medicinal Plants Documented by Ibn al-Baytar (Early 13th Century CE)
by Wendy L. Applequist
Plants 2025, 14(3), 342; https://doi.org/10.3390/plants14030342 - 23 Jan 2025
Viewed by 322
Abstract
The long-term stability of orally transmitted ethnopharmacopoeias is of interest, but difficult to study for lack of information on plants used by a specific past culture. Similarities between modern Italian ethnopharmacopoeias and Dioscorides’ classical text have been proposed to derive from modern replacement [...] Read more.
The long-term stability of orally transmitted ethnopharmacopoeias is of interest, but difficult to study for lack of information on plants used by a specific past culture. Similarities between modern Italian ethnopharmacopoeias and Dioscorides’ classical text have been proposed to derive from modern replacement of traditional practices with those from published translations of Dioscorides. Ibn al-Baytar produced the best compendium of medicinal substances in medieval Islamic science. He gave “Berber” common names for some plants, which were presumptively used by North African Amazigh people. Since Amazigh traditional knowledge was largely transmitted orally, with little access to medieval literature, this allows comparison of historic (>750 years ago) practices and modern practices that are unlikely to be causally derived. Presumptive identities for Ibn al-Baytar’s plants with Berber names were obtained from key references. Recent ethnomedicinal publications from Amazigh-populated areas in North Africa were surveyed for reports of those species and homologous common names. Of 46 historically used plants, an estimated 60.9% are still used in Amazigh regions, 78.6% with homologous common names. This is likely to underestimate persistence of species use across the entire local pharmacopoeia. Second, emulating a published analysis of Dioscorides, plants reported by three large recent studies in Amazigh regions were compared with plants recorded by Ibn al-Baytar and in a comprehensive modern Moroccan reference. Between 58.2% and 73.8% of species included in individual studies were recorded by Ibn al-Baytar; of the 46 shared among all three, 82.6% were recorded by Ibn al-Baytar and 100% by the modern reference. An historical compilation may be more likely to mention plants that are widely used today simply because a thorough author could document most plants that were widely used at the time; use of such data to assess causality should take that effect into consideration. Full article
(This article belongs to the Special Issue Historical Ethnobotany: Interpreting the Old Records—2nd Edition)
22 pages, 2067 KiB  
Article
Medicinal Plant Use in North Karelia, Finland, in the 2010s
by Renata Sõukand, Natalia Kuznetsova, Julia Prakofjewa, Sabira Ståhlberg, Ingvar Svanberg, Baiba Prūse, Giulia Mattalia and Raivo Kalle
Plants 2025, 14(2), 226; https://doi.org/10.3390/plants14020226 - 15 Jan 2025
Viewed by 463
Abstract
Finnish North Karelia is a region with a rich cultural history of ethnomedicinal plant use, shaped by centuries of interactions among various ethnic groups. This study identified both similarities and divergences between local Finns, Karelians war refugees, and individuals of mixed origin compared [...] Read more.
Finnish North Karelia is a region with a rich cultural history of ethnomedicinal plant use, shaped by centuries of interactions among various ethnic groups. This study identified both similarities and divergences between local Finns, Karelians war refugees, and individuals of mixed origin compared to historical records. Based on 67 semi-structured interviews, we documented the use of 43 medicinal plant taxa from 25 families, of which 31 remain in use. Notably, the number of medicinal plants continuously used in North Karelia is considerably lower than in other parts of Europe, with less than 25% of historically utilised species still in practice, which reflects the fragile state of this knowledge. Factors such as forced relocation, the loss of traditional lands, and the need to adapt to new environments might have contributed to this decline. Another influencing factor is official healthcare attitudes, which have prompted Finnish residents to shift from traditional herbal remedies to modern medical practices. Understanding the circulation of ethnomedicinal knowledge and its transformation over time is essential for identifying pathways to revitalise these practices within the framework of modern healthcare systems and cultural revitalisation efforts. Full article
(This article belongs to the Special Issue Historical Ethnobotany: Interpreting the Old Records—2nd Edition)
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<p>Knowledge circulation in time. (<b>a</b>) Number of taxa with different plant use periods. (<b>b</b>) Proportion of DURs in different use periods. (<b>c</b>) The Jaccard Index signals little overlap between the species used in different periods.</p>
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<p>Overlap in the ethnomedicinal knowledge of plants: (<b>a</b>) Cross-cultural comparison of all used plants. The four listed species were reported by at least 50% of the interviewees. (<b>b</b>) Correspondence between the percentage of people in the group and the percentage of the knowledge of the use of the plants they shared. The Jaccard Index indicates a relatively high diversity between the studied groups.</p>
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<p>Comparison between the numbers of the used plants (<b>a</b>) currently and (<b>b</b>) in the past between different groups of respondents (Kar, Fin, and Mix). The past use is more homogenous compared to the current use of plants. The Jaccard Index indicates highly diverse current use, while the past remembered use is more homogenous.</p>
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<p>Comparison of the currently used medicinal plants in all groups with the historical (HIST) uses that overlapped with any of the current uses. A major part of the currently used taxa was also used according to historical sources. See <a href="#sec4dot5-plants-14-00226" class="html-sec">Section 4.5</a> on how the values were calculated.</p>
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<p>A map of the study area (black square). Designed in QGIS 3.22.</p>
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22 pages, 9881 KiB  
Article
Antibacterial Activity of Traditional Medicinal Plants Used for the Treatment of Acute Diarrheal Diseases in Chiapas, Mexico
by Jesús Mauricio Ernesto Hernández-Méndez, Israel Peña-Hernández, Mariela Tapia-Nazario, Gerardo Leyva-Padrón, Abumalé Cruz-Salomón, María Emperatriz Domínguez-Espinosa, Héctor Armando Esquinca-Avilés, Maritza del Carmen Hernández-Cruz, Rocío Magdalena Sánchez-Albores, Kelly del Carmen Cruz-Salomón, Rosa Isela Cruz-Rodríguez and Martha Guadalupe Gómez-Santos
Microbiol. Res. 2025, 16(1), 10; https://doi.org/10.3390/microbiolres16010010 - 5 Jan 2025
Viewed by 941
Abstract
The Enterobacteriaceae family poses health risks due to its role in gastrointestinal diseases like acute diarrhea. With rising antibiotic resistance, plants offer promising antibacterial compounds with low toxicity. This study evaluated the antibacterial activity, minimum inhibitory concentration (MIC), and toxicity of ethanolic (EE) [...] Read more.
The Enterobacteriaceae family poses health risks due to its role in gastrointestinal diseases like acute diarrhea. With rising antibiotic resistance, plants offer promising antibacterial compounds with low toxicity. This study evaluated the antibacterial activity, minimum inhibitory concentration (MIC), and toxicity of ethanolic (EE) and aqueous (AE) extracts from five Mexican medicinal plants traditionally used in Chiapas for treating acute diarrheal diseases (ADD). Antibacterial activity was assessed using disk diffusion assays and MIC determined by macrodilution. Toxicity tests were performed using Artemia salina. As a result, EE extracts exhibited higher antibacterial activity than AE extracts. Byrsonima crassifolia effectively inhibited Salmonella enteritidis (78.26%, MIC 50 mg/mL) and Shigella dysenteriae (76.19%, MIC 25 mg/mL). Solanum torvum showed efficacy against Escherichia coli (55.55%, MIC 12.5 mg/mL) and Salmonella enteritidis (73.91%, MIC 25 mg/mL). Euphorbia maculata inhibited Shigella dysenteriae (104.76%, MIC 25 mg/mL), while Guazuma ulmifolia and Bursera simaruba exhibited no antibacterial effects. All extracts were non-toxic (LD50 > 1000 μg/mL), indicating potential as natural alternatives for ADD treatment. Full article
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<p>Location of the different collection places for plant species from Tuxtla Gutierrez, Chiapas, Mexico. (A) <span class="html-italic">Solanum torvum</span> (ST); (B) <span class="html-italic">Guazuma ulmifolia</span> (GU); (C) <span class="html-italic">Byrsonima crassifolia</span> (BC); (D) <span class="html-italic">Bursera simaruba</span> (BS); (E) <span class="html-italic">Euphorbia maculata</span> (EM).</p>
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<p>Percentage of the surveyed population using medicinal plants according to (<b>a</b>) gender and (<b>b</b>) age range.</p>
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<p>Photos of plants collected in Tuxtla Gutiérrez, Chiapas, Mexico in their wild form (self-authored). <span class="html-italic">Byrsonima crassifolia</span> (BC), <span class="html-italic">Solanum torvum</span> (ST), <span class="html-italic">Euphorbia maculate</span> (EM), <span class="html-italic">Guazuma ulmifolia</span> (GU), and <span class="html-italic">Bursera simaruba</span> (BS).</p>
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<p>Extraction yield of the most popular medicinal plants used for the treatment of diarrheal diseases in Tuxtla Gutierrez, Chiapas, Mexico. The type of extracts were aqueous extracts (AE) and ethanolic extracts (EE). Each value of the bars represents the mean ± SD, (n = 3). The letters on the bars (A–F) are significantly different (<span class="html-italic">p</span> &lt; 0.05) for the different extraction efficiency as determined by two-way ANOVA followed by Tukey’s test.</p>
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<p>Effect (inhibition halos) of aqueous extracts (AE) of medicinal plants against bacteria causing acute diarrheal diseases. Bacteria: <span class="html-italic">Escherichia coli</span> (<span class="html-italic">EC</span>), <span class="html-italic">Salmonella enteritidis</span> (<span class="html-italic">SE</span>), and <span class="html-italic">Shigella dysenteriae</span> (<span class="html-italic">SD</span>). Medicinal plant extract: <span class="html-italic">Byrsonima crassifolia</span> (<span class="html-italic">BC</span>), <span class="html-italic">Solanum torvum</span> (<span class="html-italic">ST</span>), and <span class="html-italic">Euphorbia maculate</span> (<span class="html-italic">EM</span>).</p>
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<p>Effect (inhibition halos) of ethanolic extracts (EE) of medicinal plants against bacteria causing acute diarrheal diseases. Bacteria: <span class="html-italic">Escherichia coli</span> (<span class="html-italic">EC</span>), <span class="html-italic">Salmonella enteritidis</span> (<span class="html-italic">SE)</span>, and <span class="html-italic">Shigella dysenteriae</span> (<span class="html-italic">SD</span>). Medicinal plant extract: <span class="html-italic">Byrsonima crassifolia</span> (<span class="html-italic">BC</span>), <span class="html-italic">Solanum torvum</span> (<span class="html-italic">ST)</span>, and <span class="html-italic">Euphorbia maculate</span> (<span class="html-italic">EM</span>).</p>
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32 pages, 2274 KiB  
Article
Old Plants for New Food Products? The Diachronic Human Ecology of Wild Herbs in the Western Alps
by Mousaab Alrhmoun, Aurora Romano, Naji Sulaiman and Andrea Pieroni
Plants 2025, 14(1), 122; https://doi.org/10.3390/plants14010122 - 3 Jan 2025
Cited by 1 | Viewed by 662
Abstract
This ethnobotanical study examines the traditional knowledge and usage patterns of wild plants in the western Alps, specifically within the Ubaye and Bellino Valleys, through a comparative analysis of data collected from 1983 (published in 1990) to 2024. Our study aims to assess [...] Read more.
This ethnobotanical study examines the traditional knowledge and usage patterns of wild plants in the western Alps, specifically within the Ubaye and Bellino Valleys, through a comparative analysis of data collected from 1983 (published in 1990) to 2024. Our study aims to assess the change in plant usage, species diversity, and the changing roles of plants in local traditions in the western Alpine mountain ecosystems. While the 1983 survey documented medicinal uses centered around pastoralist practices, the 2024 data highlight a notable increase in the use of synanthropic plants, now utilized both medicinally and as food. Several species such as Allium sativum, Artemisia absinthium, and Urtica dioica have shown resilience and continuity in local cultural practices, maintaining medicinal, culinary, and ritual significance across the four decades. The 1983 survey documented the greatest variety of species (101), a number that decreased in subsequent studies. The 2009 survey identified 36 species not previously recorded in 1983, and the 2024 field study noted an additional 20 species. The study highlights the economic potential of several wild species in these alpine areas, such as Achillea, Artemisia, Verbascum, Veronica, Viola, Polygonum, Bunium, and Sorbus spp., which could be utilized for creating new herbal teas, artisanal beers, liqueurs, ice creams, sweets, and seasoned food products. Expanding the uses of these plants could not only preserve ethnobotanical knowledge but also stimulate local economies and support sustainable development in alpine communities. The documented temporal shifts in plant usage reflect broader cultural, ecological, and socio-economic changes, underscoring the importance of preserving biodiversity and traditional knowledge amidst ongoing environmental and societal shifts. This study underlines the need to conserve ethnobotanical heritage while adapting to the evolving landscape of the region. Future research could focus on exploring the role of these species in broader sustainability initiatives, including conservation strategies, ecosystem services, and community-based tourism while continuing to document the cultural dynamics influencing plant usage. Full article
(This article belongs to the Special Issue Plants and Peoples: Quo Vadis?)
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<p>Comparative use of herbal teas for food and medicinal purposes across studies in the western Alps; Novaretti and Lemordant (1990) [<a href="#B11-plants-14-00122" class="html-bibr">11</a>], Pieroni and Giusti (2009) [<a href="#B3-plants-14-00122" class="html-bibr">3</a>].</p>
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<p>Genera overlapping within our present study and previous studies conducted in the region; Novaretti and Lemordant (1990) [<a href="#B11-plants-14-00122" class="html-bibr">11</a>], Pieroni and Giusti (2009) [<a href="#B3-plants-14-00122" class="html-bibr">3</a>].</p>
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<p>PCA biplot and clustering analysis of wild plant species used for food and medicinal purposes in the Ubaye and Bellino Valleys across three temporal periods (1983 [<a href="#B11-plants-14-00122" class="html-bibr">11</a>], 2009 [<a href="#B3-plants-14-00122" class="html-bibr">3</a>], and the present study in 2024). Points represent individual plant species, colored and labeled by the period of data collection. Arrows (A, B, C) denote the direction of temporal shifts in plant usage patterns, illustrating the evolution of ethnobotanical knowledge over time.</p>
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<p>Location of the study areas (Ubaye and Bellino Valleys).</p>
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<p>Landscape images of Bellino (<b>A</b>) and Ubaye (<b>B</b>) Valleys, showcasing their natural features, vegetation cover, and ecological diversity (photo free: Ubaye tourism site, and Wikipedia).</p>
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16 pages, 1309 KiB  
Article
Traditional Knowledge and Efficacy Analysis of an Emerging Medicinal Food Plant: Disporopsis aspersa
by Qingyu Chen, Miaomiao Wang, Xian Hu, Jihai Zhang, Qing Zhang, Congli Xu and Chunlin Long
Foods 2025, 14(1), 72; https://doi.org/10.3390/foods14010072 - 30 Dec 2024
Viewed by 636
Abstract
Disporopsis aspersa (Hua) Engl. ex K. Krause, locally known as kucai (bitter greens) or yexiahua, is a widely consumed wild vegetable and traditional herbal medicine in western Yunnan. Despite its local significance, its nutrient composition and bioactive properties have not been investigated. [...] Read more.
Disporopsis aspersa (Hua) Engl. ex K. Krause, locally known as kucai (bitter greens) or yexiahua, is a widely consumed wild vegetable and traditional herbal medicine in western Yunnan. Despite its local significance, its nutrient composition and bioactive properties have not been investigated. This study aims to determine the nutritional content and evaluate the antioxidant and anti-inflammatory activities of the aerial parts extracts of D. aspersa. The levels of protein, amino acids, vitamins, and minerals were measured and compared to those of common vegetables. The results showed that D. aspersa contains 16 amino acids, with a total content of up to 19.13 g/100 g, including 3.0 g/100 g of lysine. In vitro evaluations of its antioxidant and anti-inflammatory activities demonstrated that the ethanolic extract exhibited low cytotoxicity against mouse RAW 264.7 murine macrophages cell line at concentrations of 0–120 μg/mL. The IC50 for nitric oxide (NO) scavenging activity was 72.7 ± 7.43 μg/mL, showing dose dependence. Additionally, the ethanolic extract also exhibited ABTS+· scavenging capacity and total antioxidant capacity. These findings suggest that D. aspersa is rich in carbohydrates, fat, dietary fiber, and amino acids. It also contains various bioactive substances, supporting its traditional practices for both medicinal and dietary purposes by local people. D. aspersa has the potential to be developed into a novel anti-hypertensive food, nutraceutical, or dietary supplement in western Yunnan and neighboring regions, promoting local development. Full article
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<p>The plant of <span class="html-italic">Disporopsis aspersa</span> in the study area. (<b>A</b>) Aboveground part sold on market; (<b>B</b>) mature leaves; (<b>C</b>,<b>D</b>) stir-fried dish of <span class="html-italic">D. aspersa</span> showing flower buds (photographed credit: the authors).</p>
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<p>Effects of the EE of <span class="html-italic">Disporopsis aspersa</span> on RAW 264.7 cell viability. Results are expressed as percentage of cell viability relative to untreated control cells (Control). Each bar shows mean ± SD of three independent experiments performed in triplicate (*** <span class="html-italic">p</span> &lt; 0.01 compared to Control).</p>
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<p>Effects of the EE on RAW 264.7 cells NO production induced by LPS, expressed as mean ± standard deviation (<span class="html-italic">n</span> = 3). Bars with different letters indicate significant difference (<span class="html-italic">p</span> &lt; 0.05).</p>
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53 pages, 6648 KiB  
Article
Quantitative Ethnobotany of Medicinal Plants from Darjeeling District of West Bengal, India, along with Phytochemistry and Toxicity Study of Betula alnoides Buch.-Ham. ex D.Don bark
by Yasodha Subba, Samik Hazra and Chowdhury Habibur Rahaman
Plants 2024, 13(24), 3505; https://doi.org/10.3390/plants13243505 - 16 Dec 2024
Viewed by 984
Abstract
This study offers considerable information on plant wealth of therapeutic importance used traditionally by the residents of 11 villages under three subdivisions of Kurseong, Darjeeling Sadar, and Mirik in the Darjeeling District, West Bengal. For the acquisition of ethnomedicinal information, semi-structured interviews were [...] Read more.
This study offers considerable information on plant wealth of therapeutic importance used traditionally by the residents of 11 villages under three subdivisions of Kurseong, Darjeeling Sadar, and Mirik in the Darjeeling District, West Bengal. For the acquisition of ethnomedicinal information, semi-structured interviews were conducted with 47 informants, of whom 11 persons were herbalists and 36 were knowledgeable persons. Free prior informed consent was obtained from each participant prior to the collection of field data. A total of 115 species were documented, which spread over 65 families and 104 genera. From the informants, a total of 101 monoherbal and 21 polyherbal formulations were recorded for treating 50 types of health conditions. The collected ethnobotanical data have been evaluated to measure the utilitarian significance of remedies using three quantitative tools, informant consensus factor (Fic), use value (UV), and fidelity level (FL%). A statistical analysis revealed that among 11 disease categories, the highest Fic value was estimated for the category of digestive diseases. The plant Hellenia speciosa (J.Koenig) S.R.Dutta scored the highest use value among all the recorded plant species. In the case of the FL% analysis, the highest score (97%) was observed in Betula alnoides Buch-Ham. ex D.Don, which is used for snake bites, among the recorded 115 plant species. In addition, the present study embodies the quantitative estimation of phenolics and flavonoids, along with an HPLC analysis of the B. alnoides bark to endorse this most important and underexplored plant as a potential source of therapeutically important chemical compounds. The bark extract contains significant amounts of phenolics (87.8 mg GAE/g dry tissue) and flavonoids (30.1 mg CE/g dry tissue). An HPLC analysis unveiled a captivating ensemble of six phenolic compounds, namely, chlorogenic acid, sinapic acid, caffeic acid, coumarin, p-coumaric acid, and gallic acid. Among the identified phenolics, chlorogenic acid scored the highest amount of 117.5 mg/g of dry tissue. The present study also explored the moderate cytotoxic nature of the bark extract through an in vitro cytotoxicity assay on the L929 mouse fibroblast cell line. Our study not only documents the statistically analyzed information about ethnomedicinal practices that prevailed in the rural communities of the Darjeeling District but also highlights the profound therapeutic capabilities and non-toxic nature of B. alnoides bark. Full article
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<p>Number of plant species in respective families.</p>
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<p>Habits of medicinal plants in the study area.</p>
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<p>Plants parts used in herbal preparations.</p>
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<p>Methods of preparation of ethnomedicine.</p>
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<p>Route of administration of ethnomedicine.</p>
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<p>HPLC chromatogram obtained from methanolic bark extract of <span class="html-italic">B. alnoides</span>.</p>
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<p>Micrographs showing the morphological changes in the L929 mouse fibroblast cells before and after 24 h exposure to different concentrations (50–300 mg/L) of <span class="html-italic">B. alnoides</span> bark extract, along with negative control.</p>
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<p>Map (<b>a</b>–<b>d</b>). (<b>a</b>) Map showing the geographical location of West Bengal in India. (<b>b</b>) Geographical location of Darjeeling District in West Bengal. (<b>c</b>) The map of surveyed area. (<b>d</b>) Map depicting the various villages visited during the present study in Darjeeling District, West Bengal. (The map is created using Qgis software 3.10. and Google Earth Pro 7.3).</p>
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22 pages, 3197 KiB  
Article
What Drives Herbal Traditions? The Influence of Ecology and Cultural Exchanges on Wild Plant Teas in the Balkan Mountains
by Mousaab Alrhmoun, Naji Sulaiman and Andrea Pieroni
Land 2024, 13(12), 2146; https://doi.org/10.3390/land13122146 - 10 Dec 2024
Viewed by 762
Abstract
This study investigates the ethnobotanical diversity of wild plant teas across various populations in the Balkan mountain regions, focusing on cultural and ecological influences. Data were collected through ethnobotanical field surveys conducted in thirteen distinct areas, encompassing approximately 900 interviews with local populations, [...] Read more.
This study investigates the ethnobotanical diversity of wild plant teas across various populations in the Balkan mountain regions, focusing on cultural and ecological influences. Data were collected through ethnobotanical field surveys conducted in thirteen distinct areas, encompassing approximately 900 interviews with local populations, including Albanians, Bosniaks, North Macedonians, Goranis, and Serbs. The study identifies 143 genera used in traditional teas, with species frequency and distribution patterns analysed concerning elevation and cultural factors. Statistical analyses using Principal Component Analysis (PCA) and Cluster Analysis revealed clear grouping patterns between populations based on ecological zones. The results show a clear correlation between altitude and the herbal use of plant genera, with higher elevations (above 700 m) supporting a greater variety of local species. Cultural practices, such as tea consumption during communal gatherings, also significantly shaped the selection of plants across different populations. For instance, Sideritis species were most prevalent among Albanian and North Macedonian communities, while Thymus and Mentha species were widely used across all groups. Moreover, cultural exchanges can explain the similarity of wild teas reported by Bosniaks (in Bosnia) and North Albanians, located far away from each other but showing similar traits due to possible common origins or the effect of centuries of pastoralism, which may have generated a constant exchange of local plant practices. The study concludes that ecological conditions and cultural exchanges significantly influence the choice, use, and resilience of wild plant herbal traditions in the Balkans. However, modern pressures such as urbanisation and market-driven consumption are increasingly threatening these practices. This highlights the need for conservation strategies that integrate ecological preservation and cultural heritage safeguarding. Full article
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<p>Locations of the thirteen Western Balkan areas where the ethnobotanical studies were conducted and considered for the comparative analysis (see <a href="#land-13-02146-t001" class="html-table">Table 1</a>).</p>
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<p>Locations of the thirteen Western Balkan areas where the ethnobotanical studies were conducted and considered for the comparative analysis (see <a href="#land-13-02146-t001" class="html-table">Table 1</a>).</p>
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<p>Hierarchical clustering dendrogram of Balkan sites based on plant species distribution. The dendrogram illustrates three distinct clusters of sites: Cluster 1 (Prokoško Lake, Upper Kelmendi, Theth), Cluster 2 (Gora, Pešter, Albanian Alps, North Macedonia), and Cluster 3 (Kopaonik, Reka NW Macedonia, Dragash, Shtërpcë/Štrpce). The height correlations for the clusters are 0.8–0.9, 0.55–0.7, and 0.62–0.78, respectively. These clusters demonstrate the influence of geographic and ecological factors on species diversity across different elevations and regions.</p>
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<p>(<b>A</b>) PCA score plot of Balkan sites based on plant genera composition. The plot shows the grouping of sites (Group 1: Prokoško Lake, Upper Kelmendi, Theth; Group 2: Gora, Pešter, Albanian Alps, North Macedonia; Group 3: Kopaonik, Reka, NW Macedonia, Dragash, and Shtërpcë/Štrpce) according to the first two principal components. The clustering highlights the influence of ecological and geographical factors on the distribution of plant species across different elevations. (<b>B</b>): PCA loading plot of plant species distribution in Balkan sites. This plot visualises the contribution of various plant genera to the first two principal components, indicating which species are most influential in differentiating the regions.</p>
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<p>Heatmap of the correlation matrix of genera distribution across different regions, annotated by groupings identified in the dendrogram (<a href="#land-13-02146-f003" class="html-fig">Figure 3</a>). Group 1: Prokoško Lake, Upper Kelmendi, Theth; Group 2: Gora, Pešter, Albanian Alps, North Macedonia; Group 3: Kopaonik, Reka NW Macedonia, Dragash, Shtërpcë/Štrpce. (<b>A</b>) The colour gradient indicates the degree of correlation between regions, with warmer colours representing higher correlations (e.g., Group 1) and cooler colours representing lower correlations. (<b>B</b>) Numerical values denote specific correlation coefficients.</p>
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26 pages, 697 KiB  
Review
Biodiversity, Traditional Uses, and Pharmacological Potential of Medicinal Plants of Mozambique
by Emílio Henriques Razão, Marta Sánchez, María Victoria Naval, Rosario G. Gavilán and María Pilar Gómez-Serranillos
Agriculture 2024, 14(12), 2204; https://doi.org/10.3390/agriculture14122204 - 2 Dec 2024
Viewed by 1124
Abstract
Mozambique possesses great biological diversity, with approximately 5500 plant species. Around 90% of its population uses natural resources for food and traditional medicine, being miombo forests serving as key providers for rural communities. The trade of medicinal plants also constitutes a significant economic [...] Read more.
Mozambique possesses great biological diversity, with approximately 5500 plant species. Around 90% of its population uses natural resources for food and traditional medicine, being miombo forests serving as key providers for rural communities. The trade of medicinal plants also constitutes a significant economic activity for both rural and urban households. This study aims to review ethnobotanical and ethnopharmacological studies on medicinal plants in Mozambique and explore their agricultural and conservation potential. Articles published between 2010 and 2024 were included and identified 472 medicinal plant species, 400 of which were classified to species or genus level, spanning 277 genera and 93 botanical families. The most cited families include Leguminosae (61 species), Euphorbiaceae (20), and Combretaceae (17). Notable species are Momordica balsamina, Sclerocarya birrea, Annona senegalensis, and Cassia abbreviata. Sixteen in vitro studies explored medicinal plants’ antibacterial, antimycobacterial, antimicrobial, anticancer, and antimalarial properties. Prominent results were shown by Maerua edulis (tuberculosis), Tabernaemontana elegans (malaria), and Momordica balsamina (cancer). This work underscores the critical role of natural resources in Mozambique’s primary healthcare system and highlights their agricultural and economic potential. As the first comprehensive compilation of commonly used medicinal plants in the country, it calls for sustainable cultivation practices and further research to enhance their use and benefits. Full article
(This article belongs to the Section Crop Protection, Diseases, Pests and Weeds)
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<p>Frequency of part of the plant used.</p>
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25 pages, 6918 KiB  
Article
From Green Chemistry to Healthy Environments: Silver Nanoparticles as a Dual Antioxidant and Antibacterial Agents for Advancing Biomedicine and Sustainable Wastewater Treatment
by Hamza Moussa, Sarah Hamid, Amal Mameri, Sabrina Lekmine, Hichem Tahraoui, Mohammed Kebir, Nabil Touzout, Farid Dahmoune, Mohammad Shamsul Ola, Jie Zhang and Abdeltif Amrane
Bioengineering 2024, 11(12), 1205; https://doi.org/10.3390/bioengineering11121205 - 28 Nov 2024
Cited by 2 | Viewed by 1269
Abstract
The green synthesis of silver nanoparticles (AgNPs) using plant extracts is an eco-friendly method with potential for biomedical and environmental applications. This study aims to synthesize silver nanoparticles (SO-AgNPs) using Salvia officinalis L. extract and evaluate their antioxidant and antibacterial properties, positioning them [...] Read more.
The green synthesis of silver nanoparticles (AgNPs) using plant extracts is an eco-friendly method with potential for biomedical and environmental applications. This study aims to synthesize silver nanoparticles (SO-AgNPs) using Salvia officinalis L. extract and evaluate their antioxidant and antibacterial properties, positioning them as candidates for applications in sustainable biomedicine and wastewater treatment. S. officinalis L. extract was used to synthesize AgNPs under optimized conditions, with a 10% extract/AgNO₃ ratio and a reaction time of 180 min. The SO-AgNPs were characterized using ATR-FTIR, XRD, SEM, DLS, and Zeta potential analysis. The antioxidant activity of the extract and SO-AgNPs was evaluated using ABTS+• and DPPH radical scavenging assays. Antibacterial activity was tested against 11 bacterial strains and bacteria isolated from industrial effluent, with minimal inhibitory concentrations (MIC) determined for both the extract and SO-AgNPs. The SO-AgNPs demonstrated potent antioxidant activity, with IC₅₀ values of 0.233 mg/mL and 0.305 mg/mL in the ABTS+• assay, and 0.173 mg/mL and 0.185 mg/mL in the DPPH assay for the extract and SO-AgNPs, respectively. Antibacterial testing showed MIC values of 0.25 mg/mL for SO-AgNPs and between 3.12 and 6.25 mg/mL for S. officinalis L. extract against E. coli, P. aeruginosa, A. baumannii, MRSA, B. cereus, and S. epidermidis. For bacteria isolated from industrial effluent, the MIC values were 0.125 mg/mL for SO-AgNPs and 0.5 mg/mL for the extract. This study highlights the dual antioxidant and antibacterial capabilities of S. officinalis L. extract and SO-AgNPs, demonstrating their potential for use in both biomedical and environmental applications, including wastewater treatment. Full article
(This article belongs to the Special Issue Nano–Bio Interface)
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<p>Biosynthesis of SO-AgNPs over time using <span class="html-italic">S. officinalis</span> L. extract.</p>
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<p>Extract/Silver nitrate ratio effect on the biosynthesis of SO-AgNPs at 180 min.</p>
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<p>Time effect on the biosynthesis of SO-AgNPs using a 10% extract/AgNO<sub>3</sub> ratio.</p>
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<p>ATR-FTIR spectrum of <span class="html-italic">Salvia</span> extract and SO-AgNPs obtained under optimal conditions of synthesis.</p>
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<p>XRD pattern of SO-AgNPs, SN (Silver nitrate), and SE (<span class="html-italic">Salvia</span> extract) obtained under optimal conditions of synthesis.</p>
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<p>Zeta potential analysis of SO-AgNPs.</p>
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<p>SEM microscopy micrographs of SO-AgNPs captured under varied imaging magnifications, (<b>a</b>) ×30,000, HFW: 9.95 μm, WD: 10.8 mm, (<b>b</b>) ×20,000, HFW: 14.9 μm, WD: 11.4 mm, (<b>c</b>) ×12,000, HFW: 24.9 μm, WD: 9.2 mm, (<b>d</b>) ×12,000, HFW: 24.9 μm, WD: 11.4 mm, (<b>e</b>) ×3000, HFW: 99.5 μm, WD: 9.5 mm, (<b>f</b>) ×700, HFW: 426.0 μm, WD: 9.2 mm. The red circles highlighting the SO-AgNPs at each magnification level.</p>
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<p>Antioxidant activity of both <span class="html-italic">S. officinalis</span> L. extract and SO-AgNPs against DPPH<sup>•</sup> (<b>a</b>) and ABTS<sup>+•</sup> (<b>b</b>) free radical.</p>
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<p>Antimicrobial activity of <span class="html-italic">S. officinalis</span> L. extract (<b>a</b>,<b>c</b>,<b>e</b>) and SO-AgNPs (<b>b</b>,<b>d</b>,<b>f</b>) against <span class="html-italic">A. baumannii</span>, <span class="html-italic">B. cereus</span>, and <span class="html-italic">B. subtilis</span>, respectively. The red circles indicate the zones of inhibition surrounding the tested samples and the negative control.</p>
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<p>Antimicrobial activity of <span class="html-italic">S. officinalis</span> L. extract (<b>a</b>,<b>c</b>,<b>f</b>) and SO-AgNPs (<b>b</b>,<b>d</b>,<b>e</b>) against <span class="html-italic">Salmonella</span> spp., <span class="html-italic">E. coli</span>, and <span class="html-italic">E. faecalis</span>, respectively. The red circles indicate the zones of inhibition surrounding the tested samples and the negative control.</p>
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<p>Antimicrobial activity of <span class="html-italic">S. officinalis</span> L. extract (<b>a</b>,<b>c</b>,<b>e</b>) and SO-AgNPs (<b>b</b>,<b>d</b>,<b>f</b>) against <span class="html-italic">MRSA</span>, <span class="html-italic">P. aeruginosa</span>, and <span class="html-italic">K. pneumoniae</span>, respectively. The red circles indicate the zones of inhibition surrounding the tested samples and the negative control.</p>
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<p>Antimicrobial activity of <span class="html-italic">S. officinalis</span> L. extract (<b>a</b>,<b>c</b>) and SO-AgNPs (<b>b</b>,<b>d</b>) against <span class="html-italic">S. epidermidis</span>, and <span class="html-italic">S. aureus</span>, respectively. The red circles indicate the zones of inhibition surrounding the tested samples and the negative control.</p>
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<p>The MIC of SO-AgNps (<b>a</b>–<b>c</b> (S<sub>6</sub>)) and <span class="html-italic">Salvia</span> extract (c S<sub>1</sub>-S<sub>2</sub>, <b>d</b>,<b>e</b>) using the tetrazolium red salt (TTC) colorimetric assay, C<sub>1</sub>–C<sub>9</sub> are the concentration of <span class="html-italic">salvia</span> extract (25–0.976 mg/mL) and SO-AgNps (0.5–0.001 mg/mL), N is the negative control, S<sub>1</sub> is <span class="html-italic">E. coli</span>, S<sub>2</sub> is <span class="html-italic">P. aeruginosa</span>, S<sub>3</sub> is <span class="html-italic">A. baumannii</span>, S<sub>4</sub> is <span class="html-italic">MRSA</span>, S<sub>5</sub> is <span class="html-italic">B. cereus</span>, S<sub>6</sub> is <span class="html-italic">S. epidermidis</span>.</p>
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19 pages, 1256 KiB  
Article
Is Boiling Bitter Greens a Legacy of Ancient Crete? Contemporary Foraging in the Minoan Refugium of the Lasithi Plateau
by Mousaab Alrhmoun, Naji Sulaiman, Shiekh Marifatul Haq, Syed Abidullah, Julia Prakofjewa, Nikos Krigas, Andrea Pieroni and Renata Sõukand
Foods 2024, 13(22), 3588; https://doi.org/10.3390/foods13223588 - 10 Nov 2024
Cited by 1 | Viewed by 1008
Abstract
Wild greens (WGs) play a significant role in Mediterranean diets (MDs), reflecting botanical and cultural diversities, mainly influenced by a complex conglomerate of local human ecologies. This study investigates local ecological knowledge (LEK) linked to traditional gathering and consumption of WGs in the [...] Read more.
Wild greens (WGs) play a significant role in Mediterranean diets (MDs), reflecting botanical and cultural diversities, mainly influenced by a complex conglomerate of local human ecologies. This study investigates local ecological knowledge (LEK) linked to traditional gathering and consumption of WGs in the Lasithi Plateau of eastern Crete, where human genetic studies one decade ago showed very peculiar patterns, hypothesising that the Minoan civilisation took refuge there before it disappeared. A field ethnobotanical study was conducted to document the diversity of WGs and their detailed local culinary uses in the Lasithi area by interviewing 31 study participants. Fifty-nine folk taxa (species and subspecies) were recorded, corresponding to fifty-eight botanical taxa. A quotation index was measured to assess the cultural significance of WGs in the study areas; logistic regression analysis was adopted to understand the impact of sensory classifications of WGs and their local cooking methods. Lasithi’s foraging showed a notable prevalence of bitter-tasting WGs, which play a central role in local cognition and culinary practices. This bitterness aspect of WGs, potentially influenced by cultural preferences and genetic factors, probably suggests a connection to the ancient Lasithi’s inhabitants, i.e., Minoan dietary habits. We found that bitterness is the predominant sensory attribute in Lasithi, characterising 45.76% of the WGs. These findings underscore the complex interplay between local ecologies and biodiversity, LEK, and dietary traditions, highlighting the importance of WGs in understanding the evolution of foraging and plant culinary diversities across the Mediterranean. Full article
(This article belongs to the Special Issue The Ethnobiology of Wild Foods: 2nd Edition)
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<p>The maps show the location of Crete, the study area, and the villages explored in this study.</p>
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<p>DCA analysis showing Lasithi’s WG foraging (ar: aromatic; b: bitter; nb: non-bitter) compared with that of other Mediterranean areas (LAS: the present study; CRE: Central Crete [<a href="#B11-foods-13-03588" class="html-bibr">11</a>]; OLY: Olympos, Northern Karpathos Island, Greece [<a href="#B34-foods-13-03588" class="html-bibr">34</a>]; LEB: Lebanon [<a href="#B35-foods-13-03588" class="html-bibr">35</a>]; ASS: Assyria [<a href="#B36-foods-13-03588" class="html-bibr">36</a>]; SYR: Coastal Syria [<a href="#B19-foods-13-03588" class="html-bibr">19</a>]; SIC: Sicily [<a href="#B20-foods-13-03588" class="html-bibr">20</a>]; IKA: Ikaria Island, Greece [<a href="#B9-foods-13-03588" class="html-bibr">9</a>]; PAL: Palestine [<a href="#B37-foods-13-03588" class="html-bibr">37</a>]; ARM Armenia [<a href="#B38-foods-13-03588" class="html-bibr">38</a>]; KUR Kurdistan [<a href="#B36-foods-13-03588" class="html-bibr">36</a>]; TUN: Tunisia [<a href="#B39-foods-13-03588" class="html-bibr">39</a>].</p>
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<p>Greek regional differences in WVG culinary practices were revealed by correspondence analysis. Summary of sensory classification (b: bitter; nb: non-bitter; ar: aromatic) and local food use typologies: 1. consumed raw; 2. boiled; 3. fried and pie filling; 4. roasted; 5. cooked in omelettes; 6. cooked in mixtures; 7. salads; 8. with legumes; 9. pickled; 10. seasoning; 11. miscellaneous; 12. boiled and after pan-fried; 13. consumed raw or cooked (other than boiling); 14. consumed raw or boiled.</p>
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