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9 pages, 19776 KiB  
Case Report
Dermoscopy and Ultraviolet-Enhanced Fluorescence Dermoscopy (UEFD) Increase the Accuracy of Diagnosis and Are Useful in Assessing the Effectiveness of Kerion celsi Treatment
by Justyna Putek, Danuta Nowicka and Alina Jankowska-Konsur
J. Fungi 2025, 11(1), 52; https://doi.org/10.3390/jof11010052 (registering DOI) - 9 Jan 2025
Abstract
Microsporum canis, a zoophilic dermatophyte, infects the stratum corneum and keratinized tissues like hair and nails in cats and dogs, with cats serving as the primary reservoir. Most human infections arise from animal contact. We present the case of a girl aged [...] Read more.
Microsporum canis, a zoophilic dermatophyte, infects the stratum corneum and keratinized tissues like hair and nails in cats and dogs, with cats serving as the primary reservoir. Most human infections arise from animal contact. We present the case of a girl aged 8 with skin scalp lesions persisting for two months. Several scalp lesions, with a maximum diameter of 4 cm, presented as erythematous plaques with superficial scaling, yellow crusts, and edematous areas with purulent exudate. Dermoscopy revealed yellow crusts on an erythematous background, along with white scales, pustules, broken hairs, and comma hairs. Ultraviolent-enhanced fluorescence dermoscopy (UEFD) showed slight celadon green fluorescence, which enhanced the diagnosis and further helped to monitor the treatment. The PCR test confirmed the presence of M. canis. Treatment included topical ciclopirox and oral terbinafine. Lesions on the scalp and noticeable hair regrowth were observed in the areas of hair loss after two months. Kerion celsi can result in severe alopecia. To prevent scarring associated with hair loss in children, early mycological diagnostics, supported by dermoscopy and UEFD, is recommended. Full article
(This article belongs to the Special Issue Diagnosis and Treatment of Superficial Fungal Infections)
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Figure 1
<p>The clinical presentation of <span class="html-italic">Kerion celsi</span> in an 8-year-old girl on the day of admission (left lesion: 4 cm × 4 cm, central scalp; middle: 1.5 cm × 1 cm, parietal scalp; right: 0.5 cm × 0.5 cm, parietal scalp).</p>
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<p>The dermoscopic presentation of <span class="html-italic">Kerion celsi</span> in an 8-year-old girl on the day of admission. Green arrow—white scales, pink arrow—broken hairs, red arrow—pustules, blue arrow—erythema, orange arrow—yellow crusts.</p>
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<p>The ultraviolet-enhanced fluorescence dermoscopy of <span class="html-italic">Kerion celsi</span> in an 8-year-old girl on the day of admission. Celadon green perifollicular fluorescence can be observed.</p>
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<p>The clinical presentation of tinea corporis in the mother and the younger sister of the patient: (<b>a</b>) skin lesion on the left lower limb of the mother, and (<b>b</b>) skin lesions on the trunk of the younger sister.</p>
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<p>The clinical presentation of <span class="html-italic">Kerion celsi</span> in an 8-year-old girl after one month of follow-up.</p>
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<p>The dermoscopy findings of <span class="html-italic">Kerion celsi</span> in an 8-year-old girl after one month of follow-up.</p>
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<p>The dermoscopy findings of <span class="html-italic">Kerion celsi</span> in an 8-year-old girl after two months of follow-up.</p>
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<p>The dermoscopy and ultraviolet-enhanced fluorescence dermoscopy findings of <span class="html-italic">Kerion celsi</span> in an 8-year-old girl after two months of follow-up. Green arrow—white scales with post-inflammatory hyperpigmentation, orange arrow—broken hairs, red arrow—vellus hair.</p>
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<p>The clinical presentation of <span class="html-italic">Kerion celsi</span> in an 8-year-old girl after a three-month follow-up period.</p>
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11 pages, 1275 KiB  
Article
Fleece-Lined Whelping Pools Associated with Reduced Incidence of Canine Hip Dysplasia in a Guide Dog Program
by Lynna C. Feng, Alexandra Philippine, Elizabeth Ball-Conley and Sarah-Elizabeth Byosiere
Animals 2025, 15(2), 152; https://doi.org/10.3390/ani15020152 (registering DOI) - 9 Jan 2025
Abstract
Using genetic selection, Guide Dogs for the Blind (GDB) has reduced the incidence rate of canine hip dysplasia (CHD). However, given that CHD is polygenic and multifactorial, environmental factors may impact the expression of this developmental condition. The objective of this original, correlational [...] Read more.
Using genetic selection, Guide Dogs for the Blind (GDB) has reduced the incidence rate of canine hip dysplasia (CHD). However, given that CHD is polygenic and multifactorial, environmental factors may impact the expression of this developmental condition. The objective of this original, correlational research was to investigate the relationship between the substrate in the whelping pool used from birth to 3 weeks of age and CHD diagnosis. This retrospective study included 5649 dogs (Labrador Retrievers, Golden Retrievers, and Labrador–Golden Retriever crosses) at GDB. A total of 2785 dogs were whelped between 1 July 2012 and 28 November 2015, when the whelping pools were lined with newspaper, and 2864 dogs were whelped between 5 March 2016 and 29 December 2019, when the whelping pools were lined with fleece. Bayesian regression modeling of the CHD diagnoses and PennHIP distraction index scores identified that dogs in the fleece group had a lower risk for CHD diagnosis but similar PennHIP scores compared to the newspaper group. Golden Retrievers had the highest risk of CHD diagnosis and PennHIP scores, followed by Labrador–Golden Retriever crosses and finally Labrador Retrievers, with the lowest risk of CHD diagnosis and PennHIP scores. No sex differences were identified for CHD diagnosis, but males had lower PennHIP scores than females. These results provide correlational evidence that housing-related environmental factors during the first three weeks of development—specifically, the substrate in the whelping pool—are associated with lifelong orthopedic health. Full article
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<p>Three graphs of hip dysplasia incidence rate by dogs’ whelp year split by breed group ((<b>a</b>) Labrador Retriever, (<b>b</b>) Golden Retriever, (<b>c</b>) Labrador–Golden Retriever cross). A vertical line between 2015 and 2016 on each graph reflects the two treatment groups (newspaper for puppies whelped between 2012 and 2015 and fleece for puppies whelped between 2016 and 2019). Data labels below each year show the proportion of dogs diagnosed with hip dysplasia out of all dogs of that breed who were whelped that year.</p>
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<p>CHD logistic regression coefficients with 95% posterior distributions of regression coefficients, with the shaded area reflecting the middle 50% of the simulations.</p>
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<p>PennHip linear regression coefficients with 95% posterior distributions of regression coefficients, with the shaded area reflecting the middle 50% of the simulations.</p>
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14 pages, 3295 KiB  
Article
Use of Antibiotics in Companion Animals from 133 German Practices from 2018 to 2023
by Roswitha Merle, Leonie Feuer, Katharina Frenzer, Jan-Lukas Plenio, Astrid Bethe, Nunzio Sarnino, Antina Lübke-Becker and Wolfgang Bäumer
Antibiotics 2025, 14(1), 58; https://doi.org/10.3390/antibiotics14010058 (registering DOI) - 9 Jan 2025
Viewed by 114
Abstract
Background/Objectives: While antibiotic usage in farm animals has been systematically monitored and reduced in many countries, including Germany, data on companion animals such as dogs and cats remain scarce. To address this gap, a study was conducted in Germany to analyze patterns [...] Read more.
Background/Objectives: While antibiotic usage in farm animals has been systematically monitored and reduced in many countries, including Germany, data on companion animals such as dogs and cats remain scarce. To address this gap, a study was conducted in Germany to analyze patterns of antibiotic use in dogs and cats. Methods: Antibiotic usage data were obtained from debevet, a cloud-based veterinary practice management software based in Berlin, Germany. Practices with fewer than 100 patients were excluded, and data from 2018 to 2022 were analyzed. Results: The analysis included 477,310 consultations of 78,381 dogs and 241,532 consultations of 55,729 cats across 133 veterinary practices. Antibiotics were used in 12.9% of dog consultations and 22.5% of cat consultations, with substantial variation across practices. Aminopenicillins, particularly amoxicillin, were the most commonly used antibiotics, while the highest-priority critically important antibiotics (HPCIAs) accounted for 12.4% of treatments. Follow-up treatments led to changes in antibiotic substances in 9.3% of cases, often within the first two days. Indications varied by species, with respiratory issues more frequent in cats and orthopedic problems in dogs. Body weight and breed characteristics influenced the likelihood of antibiotic treatment, with short-nosed breeds showing higher odds. Conclusions: The routine data analysis provided valuable insights into antibiotic use in dogs and cats, enabling tracking trends across species and indications over time. While some specific information was missing, the invoicing data’s completeness, the cost-effectiveness of their use, and their unbiased nature make them a robust tool for monitoring and informing legislative changes. Full article
(This article belongs to the Section Antibiotics Use and Antimicrobial Stewardship)
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<p>Percentage of consultations with antibiotic treatment per veterinary practice. The ● represents an outlier outside the range of 1.5 standard deviations.</p>
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<p>Histogram of time to follow-up consultations for dogs and cats, categorized by consultations with or without treatment with antibiotics.</p>
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<p>Percentage of consultations with antibiotic treatment per substance class in dogs and cats (145,846 applications or deliveries). Highlighted are substance groups that belong to the highest priority critically important antibiotics.</p>
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<p>Percentage of consultations per indication group in cats and dogs.</p>
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<p>Percentage of consultations with antibiotic treatment per indication group in cats and dogs.</p>
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<p>Percentage of visits with antibiotic treatment in cats and dogs over age. Displayed are the estimates of a non-linear regression model with natural splines, 4 degrees of freedom, and a 95% confidence interval; n = 610,854 consultations.</p>
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<p>Influence of body weight on the probability of being treated with antibiotics in dogs (n = 477,310 consultations). Displayed are the estimates of a non-linear regression model with natural splines, 4 degrees of freedom, and a 95% confidence interval.</p>
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13 pages, 1104 KiB  
Article
Differences in Transdiaphragmatic Pressure of Dogs Suffering from Cervical or Thoracolumbar Myelopathy Anaesthetised with Isoflurane
by Eirini Sarpekidou, Kyriaki Pavlidou, Ioannis Savvas, Zoe Polizopoulou and George Kazakos
Animals 2025, 15(2), 147; https://doi.org/10.3390/ani15020147 - 9 Jan 2025
Viewed by 103
Abstract
Background: The purpose of this study is to evaluate differences in transdiaphragmatic pressure (Pdi) in dogs suffering from cervical myelopathy (CM) and thoracolumbar myelopathy (TLM) anaesthetised with isoflurane (ISO). Methods: This prospective cohort study included ASA II-status dogs diagnosed with either CM or [...] Read more.
Background: The purpose of this study is to evaluate differences in transdiaphragmatic pressure (Pdi) in dogs suffering from cervical myelopathy (CM) and thoracolumbar myelopathy (TLM) anaesthetised with isoflurane (ISO). Methods: This prospective cohort study included ASA II-status dogs diagnosed with either CM or TLM, anaesthetised for diagnostic or surgical purposes between September 2021 and July 2023. Patients followed the same anaesthetic protocol, and maximum Pdi was recorded at 10 (Pdimax10), 20 (Pdimax20), and 30 (Pdimax30) minutes using balloon catheters placed in the stomach (Pgast) and in the mid-third of the oesophagus (Poes). Results: A total of 50 dogs were included: 25 in the CM group and 25 in the TLM group. In the TLM group, Pdimax10 was 9.4 mmHg ± 6.1, and in the CM group, it was 7.2 mmHg ± 4.6 (p = 0.167). Pdimax20 was 10.2 mmHg ± 5.8 in the TLM group and 8 mmHg ± 5.1 in the CM group (p = 0.155). Pdimax30 was 9.2 mmHg ± 5.5 in the TLM group and 8.1 mmHg ± 5.5 in the CM group (p = 0.479). Conclusions: The findings suggest that diaphragmatic contractility is similar in dogs with CM and TLM under ISO anaesthesia. Full article
(This article belongs to the Section Veterinary Clinical Studies)
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<p>Indicative depiction of the catheter positions in the mid-third of the oesophagus (catheter marked in red) and the stomach (catheter marked in blue) in a dog. The green line represents the diaphragm.</p>
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<p>Line graph of four consecutive breathing cycles of a dog. The first cycle represents the pressures of a normal respiratory cycle under general anaesthesia. The following three cycles represent the pressures after the application of Mueller’s manoeuvre. The green line represents Pdimax (Transdiaphragmatic Pressure), the blue line indicates Pgasmax (Gastric Pressure), and the red line indicates Poesmax (Oesophageal Pressure).</p>
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13 pages, 2474 KiB  
Article
Thermographic Scan of the Thoracolumbar Area in Dogs with Acute Intervertebral Disc Extrusion (IVDE): A Retrospective Study
by Cristian Zaha, Liliana Cărpinișan, Larisa Schuszler, Nistor Paula, Tudor Căsălean, Tiana Florea, Văduva Cristina, Bogdan Sicoe, Ciprian Rujescu and Roxana Dascălu
Life 2025, 15(1), 68; https://doi.org/10.3390/life15010068 (registering DOI) - 9 Jan 2025
Viewed by 108
Abstract
Background: several authors have documented variations in local temperature in both horses and dogs presenting acute intervertebral disc extrusion (IVDE) along the entire spinal column. However, none have demonstrated distinct temperature differences between healthy animals and those with IVDE. A retrospective study was [...] Read more.
Background: several authors have documented variations in local temperature in both horses and dogs presenting acute intervertebral disc extrusion (IVDE) along the entire spinal column. However, none have demonstrated distinct temperature differences between healthy animals and those with IVDE. A retrospective study was conducted to assess the efficacy of thermography at evaluating local temperature and thermal patterns in healthy dogs as well in those with IVDE across the T11–L3 area. Methods: the study included 20 healthy dogs and 32 dogs with IVDE. For both groups of dogs, the thoracolumbar region was trimmed and, subsequently, scanned using the Flir E50 thermography device. The Flir Tool software was used to analyze three designated areas (Bx1, Bx2, Bx3) within the thoracolumbar region by comparing the average temperature of the minimum, maximum, and mean temperature recordings between the two groups. Results: the thermal pattern and the local temperature of the thoracolumbar area present differences between healthy dogs and those with IVDE. Conclusions: we recommend thermographic scanning of the thoracolumbar area to find differences in local temperature between healthy dogs and those with intervertebral disc extrusion. Further investigations are required to differentiate between disc extrusion that exhibits lateralization to the right or left. Full article
(This article belongs to the Special Issue Veterinary Pathology and Veterinary Anatomy: 2nd Edition)
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Figure 1
<p>Photo of the thoracolumbar area in a paraplegic dog: (<b>a</b>) normal image from the FLIR E50 camera, (<b>b</b>) thermographic image from the FLIR E50 camera. Arrows: (<b>a</b>) yellow arrows—T7 level, red arrows—L7 level; (<b>b</b>) green arrows—T7 level, red arrows—L7 level.</p>
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<p>Computed tomography image from a dog with T13–L1 disc extrusion: (<b>a</b>) sagitally reconstructed view of the vertebral column, T13—13th thoracic vertebra, L1—1th lumbar vertebra, (<b>b</b>) dorsally reconstructed view of the vertebral column, T13—13th thoracic vertebra, L1—1th lumbar vertebra, red circle—extruded discal material.</p>
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<p>Thermographic image of the thoraco-lumbar area of a healthy dog. (<b>a</b>) Scanning image without FLIR Tools software analysis: black arrow—clipped hair area between the T7 and L7 level, green arrow—variation areas of increase in temperature, blue arrow—variation areas of low temperature, spot—local temperature of the skin. (<b>b</b>) Scanning image with FLIR Tools software analysis: black arrow—clipped hair area between the T7 and L7 level, Bx1—area of interest centered on the vertebrae, Bx2—area of interest on the right side of the vertebrae, Bx3—aria of interest on the left side of the vertebrae; red triangle spot—maximum temperature recorded in the interest area; blue triangle spot—minimum temperature recorded in the interest area.</p>
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<p>Thermographic image of the thoraco-lumbar area in a dog with T13–L1 disc extrusion. (<b>a</b>) Scanning image without FLIR Tools software analysis: black arrow—clipped hair area between the T7 and L7 level, green arrow—area of increased temperature, blue arrow—area of lower temperature, spot—local temperature of the skin. (<b>b</b>) Scanning image with FLIR Tools software analysis: black arrow—clipped hair area between the T7 and L7 level, Bx1—area of interest centered on the vertebrae, Bx2—area of interest on the right side of the vertebrae, Bx3—area of interest on the left side of the vertebrae; red triangle spot—maximum temperature recorded in the interest area; blue triangle spot—minimum temperature recorded in the interest area.</p>
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<p>Comparative boxplot referring to the results obtained for Bx1 between the study group (blue mark) and the control group (red mark); distribution of the average, maximum, and minimum temperatures; ◦—outliers.</p>
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<p>Comparative boxplot referring to the results obtained for Bx2 between the study group (blue mark) and the control group (red mark); distribution of the average, maximum, and minimum temperatures; ◦—outliers.</p>
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<p>Comparative boxplot referring to the results obtained for Bx3 between the study group (blue mark) and the control group (red mark); distribution of the average, maximum, and minimum temperatures; ◦—outliers.</p>
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18 pages, 3957 KiB  
Article
Nutritional and Microbiome Effects of a Partial Substitution of Poultry Meat with Hydrolyzed Feather Meal in Dog Diets
by Fatemeh Balouei, Bruno Stefanon, Rosangela Armone, Andrea Randazzo and Biagina Chiofalo
Microorganisms 2025, 13(1), 121; https://doi.org/10.3390/microorganisms13010121 - 9 Jan 2025
Viewed by 132
Abstract
Two extruded diets isoenergetic, isonitrogenous, and isolipidic were formulated with poultry meal (control diet) as the source of animal-origin proteins (160 g/kg of feed) or with 90 g/kg of poultry meal and 70 g/kg of hydrolyzed feather meal (treated diet) and were fed [...] Read more.
Two extruded diets isoenergetic, isonitrogenous, and isolipidic were formulated with poultry meal (control diet) as the source of animal-origin proteins (160 g/kg of feed) or with 90 g/kg of poultry meal and 70 g/kg of hydrolyzed feather meal (treated diet) and were fed to eight dogs (four adult female and four adult male English Setters). Body condition, muscle condition, and fecal consistency scores and body weight were monitored at the beginning of the trial and after 3, 7, 15, and 45 days, and no significant differences (p > 0.05) were observed between diets and between sex. Fecal samples, collected at the same time points, were analyzed for microbiota composition. No significant difference was calculated for the alpha diversity index between control diet and treated diet, nor for the diets × times of sampling interaction and for sex. Beta diversity was different (p-value 0.001) between the control and treated groups. The beta diversity between sexes was significantly different (p-value = 0.047). Linear Discriminant analysis effect size analysis revealed significant differences between dietary groups, identifying Clostridiales, Coprococcus, Bacteroides plebeius, Eubacterium biforme, Catenibacterium, and Prevotella copri as more abundant in the CTR diet, while Fusobacterium, Bacteroides, Fusobacteriaceae, Paraprevotellaceae, Enterococcus, Faecalibacterium, Enterobacteriaceae, Peptostreptococcaceae, and Clostridium spiroforme were more abundant in the treated diet. Sex differences were also significant, with 25 taxa differing between male and female dogs. Overall, the study underscores the impact of HFM and sex on gut microbiota composition in dogs, with potential implications for dietary interventions and microbiome research. Full article
(This article belongs to the Special Issue Gut Microbiota and Nutrients, 2nd Edition)
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Figure 1
<p>Relative abundance profile of phyla (Panel <b>A</b>) and families (Panel <b>B</b>) of the dogs fed with control diet (CTR) and experimental diet (TRT) at T0, T03, T07, T15, and T45. CTR group, dogs fed with diet with poultry meal; TRT group, dogs fed with diet with hydrolyzed feather meal. Sampling times: T0, T03, T07, T15, and T45 indicate samples collected at the beginning of the study and after 3, 7, 15, and 45 days.</p>
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<p>Relative abundance profile of phyla (Panel <b>A</b>) and families (Panel <b>B</b>) of the dogs fed with control diet (CTR) and experimental diet (TRT) at T0, T03, T07, T15, and T45. CTR group, dogs fed with diet with poultry meal; TRT group, dogs fed with diet with hydrolyzed feather meal. Sampling times: T0, T03, T07, T15, and T45 indicate samples collected at the beginning of the study and after 3, 7, 15, and 45 days.</p>
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<p>Comparison of the Shannon alpha diversity index of dogs across dietary treatments (Panel <b>A</b>), Diet × Time of sampling interaction (Panel <b>B</b>), and Sex (Panel <b>C</b>). CTR group, dogs fed with diet with poultry meal; TRT group, dogs fed with diet with hydrolyzed feather meal. Sampling times: T0, T03, T07, T15, and T45 indicate samples collected at the beginning of the study and after 3, 7, 15, and 45 days.</p>
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<p>Principal component analysis of the Bray–Curtis beta diversity index of dogs across dietary treatments (Panel <b>A</b>), Diet × Time of sampling interaction (Panel <b>B</b>), and Sex (Panel <b>C</b>). CTR group, dogs fed with diet with poultry meal; TRT group, dogs fed with diet with hydrolyzed feather meal. Sampling times: T0, T03, T07, T15, and T45 indicate samples collected at the beginning of the study and after 3, 7, 15, and 45 days.</p>
Full article ">Figure 3 Cont.
<p>Principal component analysis of the Bray–Curtis beta diversity index of dogs across dietary treatments (Panel <b>A</b>), Diet × Time of sampling interaction (Panel <b>B</b>), and Sex (Panel <b>C</b>). CTR group, dogs fed with diet with poultry meal; TRT group, dogs fed with diet with hydrolyzed feather meal. Sampling times: T0, T03, T07, T15, and T45 indicate samples collected at the beginning of the study and after 3, 7, 15, and 45 days.</p>
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<p>Taxa that significantly differed between the CTR and TRT diets in the Linear Discriminant Analysis Effect Size (LEfSe). CTR group, dogs fed with diet with poultry meal; TRT group, dogs fed with diet with hydrolyzed feather meal.</p>
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<p>Taxa that significantly differed in the Linear Discriminant Analysis Effect Size (LEfSe) between the CTR and TRT diets during the sampling times. CTR group, dogs fed with diet with poultry meal; TRT group, dogs fed with diet with hydrolyzed feather meal. Sampling times: T0, T03, T07, T15, and T45 denote samples collected at the beginning of the study and after 3, 7, 15, and 45 days.</p>
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<p>Taxa that significantly differed in the Linear Discriminant Analysis Effect Size (LEfSe) between male and female dogs.</p>
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11 pages, 855 KiB  
Article
A New Graphical Method for Displaying Two-Dimensional Echocardiography Results in Dogs: Comprehensive Analysis of Results of Diagnostic Imaging Organized in a BOX (CARDIOBOX)
by Federico J. Curra-Gagliano, Martín Ceballos, José I. Redondo and Javier Engel-Manchado
Vet. Sci. 2025, 12(1), 34; https://doi.org/10.3390/vetsci12010034 - 9 Jan 2025
Viewed by 132
Abstract
Introduction and objective: Rapid and efficient interpretation of echocardiographic findings is critical in clinical decision-making. This study aimed to design and validate a new graphical method, called CARDIOBOX, to represent echocardiographic findings in dogs. Methods: A prospective, observational, exploratory cohort study was conducted [...] Read more.
Introduction and objective: Rapid and efficient interpretation of echocardiographic findings is critical in clinical decision-making. This study aimed to design and validate a new graphical method, called CARDIOBOX, to represent echocardiographic findings in dogs. Methods: A prospective, observational, exploratory cohort study was conducted over three years. The design of CARDIOBOX was based on baseline values obtained from 802 healthy dogs and 2165 ill dogs. Using these data, a graph consisting of nine boxes was built to show the intervals of the different echocardiographic measurements. Validation of the method was performed by a survey of 55 veterinarians, who compared the use of CARDIOBOX with the use of numerical tables. Results: CARDIOBOX demonstrated significantly faster interpretability (p < 0.05) without reducing its effectiveness. In addition, the staff surveyed considered it easy to use and interpret. Conclusions: The introduction of CARDIOBOX emerges as a resource that facilitates rapid and efficient interpretation of echocardiographic findings in dogs. This new graphical method is presented as a valuable tool for veterinary professionals in clinical decision-making in the field of veterinary cardiology. Full article
(This article belongs to the Special Issue Advanced Therapy in Companion Animals)
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<p>Basic structure of the CARDIOBOX. A marker indicates each parameter in one of the nine boxes: boxes 1, 2, and 3 for values below the normal range (white); boxes 4, 5, and 6 for values within the normal range (grey central rectangle); and boxes 7, 8, and 9 for values above the normal range (white).</p>
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<p>Likert graphs for perceived ease of use and tool effectiveness.</p>
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12 pages, 237 KiB  
Article
Beliefs About Cats and Dogs Among Pet Owners and Former Owners
by Tiffani J. Howell, Silvana Diverio and David J. Menor-Campos
Pets 2025, 2(1), 2; https://doi.org/10.3390/pets2010002 - 8 Jan 2025
Viewed by 256
Abstract
Despite an increase in research into cat and dog cognition, behavior, and welfare in recent decades, it is unclear whether pet owners are aware of recent advances in our understanding of cats and dogs. Misunderstandings about the reasons for animal behavior can lead [...] Read more.
Despite an increase in research into cat and dog cognition, behavior, and welfare in recent decades, it is unclear whether pet owners are aware of recent advances in our understanding of cats and dogs. Misunderstandings about the reasons for animal behavior can lead to negative welfare outcomes for the animal, and potentially distress for the owner, so it is important for owners to understand the latest science on animal behavior. Current and former pet owners (N = 224) completed an online survey rating their agreement with a series of statements about cat and dog behavior. After completing the survey, participants were provided with a document describing the latest scientific knowledge about each of the statements in the survey. For both species, participant beliefs generally accord with the latest scientific knowledge, but there is evidence of remaining beliefs about aspects of dominance training theory in dogs, and the belief that cats are low-maintenance pets. These findings can be used by trainers and behaviorists to help educate owners about pet needs, working from the owner’s baseline knowledge and debunking persistent myths. Full article
11 pages, 1612 KiB  
Article
Wild Boar Attacks on Hunting Dogs in Czechia: The Length of the Hunting Season Matters
by Jana Adámková, Karolína Lazárková, Jan Cukor, Hana Brinkeová, Jitka Bartošová, Luděk Bartoš and Kateřina Benediktová
Animals 2025, 15(2), 130; https://doi.org/10.3390/ani15020130 - 8 Jan 2025
Viewed by 213
Abstract
Hunting dogs are exposed to the risk of injury in driven hunts, an often-used method for managing growing wild boar numbers. This study investigated the impact of increased hunting pressure—both across the hunting season and within individual hunting events—on the risk of wild [...] Read more.
Hunting dogs are exposed to the risk of injury in driven hunts, an often-used method for managing growing wild boar numbers. This study investigated the impact of increased hunting pressure—both across the hunting season and within individual hunting events—on the risk of wild boar attacks on hunting dogs, i.e., the length of the hunting season (2.68 ± 0.76 months, mean ± standard deviation), the number of driven hunts per season (3.99 ± 0.43), the intervals between hunts (17.85 ± 4.83 days), the number of wild boars harvested per season (14.46 ± 13.10), and the number of participants (23.8 ± 10.69) and dogs (4.56 ± 2.66) involved per hunt. The data were collected via a retrospective questionnaire survey. The information-theoretic approach (IT-AIC) and GLMM were employed to estimate the factors’ effects on the number of wild boar attacks on dogs reported in 40 hunting grounds in five consecutive hunting seasons (2.60 ± 5.07 attacks per hunting season in a hunting ground). The number of attacks only increased with the length of the hunting season. The best model did not include other factors, such as shorter intervals between hunts, a higher number of driven hunts, wild boars harvested, or participants. The respondents reported 150 injuries by wild boars during 797 driven hunts. Most injuries were mild (73.8%), with fewer severe (18.8%) and fatal (7.4%) cases. Further investigation into wild boar and hunting dog interactions is necessary for constructing strategies to improve hunting practices and reduce dog injury risks. Full article
(This article belongs to the Section Wildlife)
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<p>The study area map shows 40 hunting grounds (game management areas) with valid responses and the distribution of the forested regions and agricultural fields. EN: © EuroGeographics for the administrative boundaries. OpenStreetMap. Source service: © CENIA, česká informační agentura životního prostředí, Source data: © Agentura ochrany přírody a krajiny, Available online: Národní geoportál INSPIRE <a href="http://geoportal.gov.cz" target="_blank">http://geoportal.gov.cz</a> (accessed on 27 December 2024).</p>
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<p>Predicted values of the number of wild boar attacks reported during a hunting season on dogs (log-transformed) with 95% confidence intervals according to the hunting season length (<span class="html-italic">x</span>-axis). The bubble size refers to the number of hunting seasons the data were obtained from (n = 195).</p>
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11 pages, 2286 KiB  
Article
The Usefulness of the Kidney-to-Aorta Ratio in Dogs with Chronic Kidney Disease
by Dario Costanza, Erica Castiello, Pierpaolo Coluccia, Camilla Sangiuliano, Maria Pia Pasolini, Micheletino Matarazzo, Giacomo Gnudi, Adelaide Greco and Leonardo Meomartino
Vet. Sci. 2025, 12(1), 29; https://doi.org/10.3390/vetsci12010029 - 8 Jan 2025
Viewed by 204
Abstract
The kidney length (KL) to aortic diameter (AoD) ratio (KL/AoD) has been proposed as an ultrasonographic objective method to assess renal dimensions. However, its wide range of normal values limits sensitivity. Of note, its clinical utility in detecting renal disease has not been [...] Read more.
The kidney length (KL) to aortic diameter (AoD) ratio (KL/AoD) has been proposed as an ultrasonographic objective method to assess renal dimensions. However, its wide range of normal values limits sensitivity. Of note, its clinical utility in detecting renal disease has not been investigated. The main aim of this study was to assess the sensitivity and specificity of KL/AoD in dogs with chronic kidney disease. Of the 227 dogs in the final sample, 185 were healthy and 42 were diseased. The obtained cut-off values for healthy dogs were similar to those previously reported. No differences were found between the KL of healthy and diseased dogs; however, using the KL/AoD ratio, diseased dogs showed a significantly smaller ratio (p = 0.0003), although a partial overlap between the two groups’ values was present. Considering the Youden index at KL/AoD = 6.3, the ROC curve displayed a specificity of 83.24% and a sensitivity of 41.67%, while for KL/AoD = 5.6, the specificity was 97.57% and the sensitivity was 13.10%. The results of this study provide clinical usefulness for the KL/AoD ratio method, revealing excellent specificity but poor sensitivity. Full article
(This article belongs to the Special Issue Advanced Therapy in Companion Animals)
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<p>Dorsal ultrasonographic image of the left kidney (<b>A</b>) and of the aorta (<b>B</b>) acquired just caudal to the origin of the renal artery. In (<b>A</b>), the renal length is measured at the point of maximal cranio-caudal renal length (indicated by the measurement cursors with number 1). In (<b>B</b>), the maximal luminal diameter is obtained after reviewing cineloop frames to account for pulsation of the aorta; measurement cursors were placed at the margin of the lumen, excluding the vessel walls.</p>
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<p>Box and whisker plot of one-way ANOVA of (<b>A</b>) KL (in cm), (<b>B</b>) AoD (in cm), and (<b>C</b>) Kruskal–Wallis test of KL/AoD ratio, for toy-, small-, medium-, and large-sized sub-groups. The line within the box represents the median, while the cross the mean, upper and lower sides of the box are the lower and upper quartiles, and the two extreme horizontal lines represent 5–95 percentiles. The asterisks (*) in Figure (<b>C</b>) indicate the statistically significant differences.</p>
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<p>Correlation between body weight (in kg) and (<b>A</b>) KL (in cm), (<b>B</b>) AoD (in cm), and (<b>C</b>) KL/AoD ratio. The red solid line represents the simple linear regression.</p>
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<p>Box and whisker plot comparing (<b>A</b>) <b>the</b> kidney length (in cm) and (<b>B</b>) the KL/AoD ratio in healthy and diseased dogs. The line within the box represents the median, while the cross the mean, upper and lower sides of the box are the lower and upper quartiles, and the two extreme horizontal lines represent 5–95 percentiles.</p>
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<p>Receiver operating characteristic (ROC) curve of KL/AoD ratio.</p>
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12 pages, 1833 KiB  
Article
Fourier Transformation-Based Analysis of X-Ray Diffraction Pattern of Keratin for Cancer Detection
by Alexander Alekseev, Oleksii Avdieiev, Sasha Murokh, Delvin Yuk, Alexander Lazarev, Daizie Labelle, Lev Mourokh and Pavel Lazarev
Crystals 2025, 15(1), 57; https://doi.org/10.3390/cryst15010057 - 8 Jan 2025
Viewed by 208
Abstract
With the growing number of cancer cases and deaths around the world, fast, non-invasive, and inexpensive screening is paramount. We examine the feasibility of such cancer detection using the X-ray scattering properties of nails in the canine model. A total of 945 samples [...] Read more.
With the growing number of cancer cases and deaths around the world, fast, non-invasive, and inexpensive screening is paramount. We examine the feasibility of such cancer detection using the X-ray scattering properties of nails in the canine model. A total of 945 samples taken from 266 dogs were measured, with 84 animals diagnosed with cancer. To analyze the obtained X-ray diffraction patterns of keratin, we propose a method based on the two-dimensional Fourier transformation of the images. We compare 745 combinations of data preprocessing steps and machine learning classifiers and determine the corresponding performance metrics. Excellent classification results are demonstrated, with sensitivity or specificity achieving 100% and the best value for balanced accuracy being 87.5%. We believe that our approach can be extended to human samples to develop a non-invasive, convenient, and cheap method for early cancer detection. Full article
(This article belongs to the Section Biomolecular Crystals)
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<p>(<b>Left</b>) the nail before cutting. (<b>Right</b>) the surgical knife with the sample.</p>
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<p>The preprocessed XRD images: (<b>a</b>) the image after calibration; (<b>b</b>) the image after centering, rotation, removing hot pixels, and normalization.</p>
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<p>Dependence of the intensity on momentum transfer after the azimuthal integration of the XRD patterns: (<b>a</b>) 360-degree integration; (<b>b</b>) integration over the horizontal sectors; (<b>c</b>) integration over the vertical sectors.</p>
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<p>ROC curves for various preprocessing steps and machine learning algorithms (the terminology is described in <a href="#sec2-crystals-15-00057" class="html-sec">Section 2</a>): (<b>a</b>) <span class="html-italic">2DF</span>, <span class="html-italic">LPF</span>, <span class="html-italic">BRF</span>, <span class="html-italic">Im</span>, and <span class="html-italic">RF</span> (<span class="html-italic">STD</span> and <span class="html-italic">PCA_100</span> for the red curve and no <span class="html-italic">STD</span> and <span class="html-italic">PCA_100</span> for the blue curve); (<b>b</b>) <span class="html-italic">2DF</span> and <span class="html-italic">Am</span> (<span class="html-italic">LOG</span> for the blue curve and <span class="html-italic">RF</span> for the red curve).</p>
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<p>PCA-transformed data in 3 dimensions for <span class="html-italic">2DF</span>, <span class="html-italic">LPF</span>, <span class="html-italic">STD</span>, and <span class="html-italic">Am</span> with (<b>a</b>) <span class="html-italic">BRF</span> and (<b>b</b>) <span class="html-italic">BR</span>.</p>
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10 pages, 1658 KiB  
Article
Toxoplasma gondii and Rabies—The Parasite, the Virus, or Both?
by Ragan Wilson, Shannon Caseltine, Edith Will, Jeremiah Saliki and Ruth C. Scimeca
Microorganisms 2025, 13(1), 109; https://doi.org/10.3390/microorganisms13010109 - 8 Jan 2025
Viewed by 286
Abstract
Toxoplasma gondii is an intracellular protozoan parasite that infects a wide range of vertebrates, including humans. Although cats are the only definitive host, any warm-blooded animal can act as a paratenic host. Throughout the years, this apicomplexan parasite has been studied due to [...] Read more.
Toxoplasma gondii is an intracellular protozoan parasite that infects a wide range of vertebrates, including humans. Although cats are the only definitive host, any warm-blooded animal can act as a paratenic host. Throughout the years, this apicomplexan parasite has been studied due to its wide prevalence, zoonotic potential, and host behavioral alterations. Known for its neurological alterations, the rabies virus is one of the most recognized types of zoonosis that, although preventable, still causes deaths in humans and animals worldwide. Due to the overlapping clinical signs of these two pathogens, the objective of this study was to evaluate the prevalence of T. gondii DNA in cerebellum tissue collected for rabies testing; cerebellum tissue from diverse animals is often submitted for this purpose. Between May 2022 and April 2024, we tested 903 cerebellum tissue samples from 22 animal species submitted for rabies testing to the Oklahoma Animal Diagnostic Disease Laboratory. Overall, T. gondii prevalence was 3.96%, with 1.8% found in cats (Felis catus), 1.7% in dogs (Canis familiaris), 0.3% in skunks (Mephitis mephitis), and 0.2% in infected cattle (Bos taurus). Analysis among T. gondii-positive hosts revealed a statistically significant difference in dogs when comparing neutered vs. intact males, with 7.94% (5/63) T. gondii-positive neutered males and 1.61% (3/186) T. gondii-positive intact males (p = 0.02). All the T. gondii-positive samples were negative for rabies. Anamnesis in some of the T. gondii-positive samples included ataxia, aggression, muscle rigidity, lethargy, and seizures, with the latter also described in dogs and aggression in the positive bovine sample. The clinical signs described in the T. gondii-infected hosts can be mistaken for rabies infection; therefore, it is important to consider T. gondii as a differential diagnosis in suspected rabies cases and test for this parasite when negative rabies results are obtained. Full article
(This article belongs to the Special Issue Rabies Virus: Infections, Reservoirs and Vectors)
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<p><span class="html-italic">Toxoplasma gondii</span> positive cases displayed by location in the state of Oklahoma.</p>
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<p>Rabies positive cases displayed by location in the state of Oklahoma.</p>
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<p>Infected and non-infected hosts that tested positive for <span class="html-italic">T. gondii.</span> according to sex.</p>
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<p>Infected and non-infected hosts that tested positive for rabies according to sex.</p>
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13 pages, 707 KiB  
Article
Data Platform for Animal Mortality Information System (DATASIMA): Monitoring Companion Animal’s Euthanasia Causes in City of João Pessoa, Brazil
by Eduardo S. S. Sousa, Maria E. S. Sousa, Moisés D. C. A. Pereira, Ricardo A. M. Negreiros, Lilian R. C. Eloy, Arthur W. L. Brasil, Inácio J. Clementino, Sérgio S. Azevedo and Ricardo B. Lucena
Vet. Sci. 2025, 12(1), 28; https://doi.org/10.3390/vetsci12010028 - 8 Jan 2025
Viewed by 303
Abstract
The literature regarding causes of animal mortality varies greatly in how it evaluates and describes reasons for euthanasia, showing a clear need for tools to identify, standardize, and map diseases. This study describes the application of the Animal Mortality Information System Database (DATASIMA) [...] Read more.
The literature regarding causes of animal mortality varies greatly in how it evaluates and describes reasons for euthanasia, showing a clear need for tools to identify, standardize, and map diseases. This study describes the application of the Animal Mortality Information System Database (DATASIMA) to monitoring and georeferencing animal mortality. An observational study was conducted on the dogs and cats euthanized at the Municipal Center for Environmental and Zoonotic Surveillance in João Pessoa, Paraíba. The deaths were reported through DATASIMA, an online platform created and developed in Brazil, which is aimed at observing animal mortality through the Animal Death Declaration between April and September 2022. A total of 403 euthanized animals were recorded, including 204 dogs and 199 cats. The most common diseases leading to euthanasia were as follows: (i) Sporotrichosis (171 cats); (ii) Leishmaniasis (75 dogs); (iii) Distemper (35 dogs); (iv) Trauma from vehicle collisions (ten dogs and nine cats). The DATASIMA platform proved to be an effective system for keeping records and reporting causes of animal mortality. Through georeferencing, it was possible to map areas with higher frequencies of zoonotic outbreaks in the municipality studied, thus providing useful data to implement health promotion strategies. Full article
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<p>Georeferencing of the frequency of the main causes of euthanasia in dogs identified by the DATASIMA platform, according to the neighborhood/territory of the municipality of João Pessoa, Paraíba, Brazil. The top five neighborhoods for each condition/disease are listed below.</p>
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12 pages, 249 KiB  
Article
Behavioral and Physiological Responses of Therapy Dogs to Animal-Assisted Treatment in an Inpatient Stroke Rehabilitation Program
by Hao-Yu Shih, François Martin, Debra Ness, Whitney Romine, Taylor L. Peck, Tricia Turpin, Rachael Horoschak, Cindy Steeby, Hannah Phillips, Mary Claypool, Amanda Theuer, Grace M. Herbeck, Jasmine Sexton, Erin Pittman, Erica Bellamkonda, Nikita Maria Ligutam Mohabbat, Sandra A. Lyn, Brent A. Bauer and Arya B. Mohabbat
Animals 2025, 15(2), 121; https://doi.org/10.3390/ani15020121 - 7 Jan 2025
Viewed by 508
Abstract
Therapy dogs have been increasingly incorporated into a variety of medical treatment programs to improve patients’ treatment outcomes and wellbeing. However, research investigating the stress level of therapy dogs in this setting is limited. This is the first randomized–controlled and prospective study that [...] Read more.
Therapy dogs have been increasingly incorporated into a variety of medical treatment programs to improve patients’ treatment outcomes and wellbeing. However, research investigating the stress level of therapy dogs in this setting is limited. This is the first randomized–controlled and prospective study that investigated the wellbeing of therapy dogs in an inpatient stroke rehabilitation program. In this study, 14 therapy dog–handler pairs were embedded in an inpatient stroke rehabilitation program to provide animal-assisted treatment (AAT). These therapy dog–handler pairs actively participated in stroke rehabilitation by walking with the patient, playing fetch with the patient, and being petted/brushed by the patient, amongst various other AAT activities. To measure canine stress responses during the rehabilitation sessions, salivary cortisol and oxytocin concentrations, heart rate and heart rate variability, tympanic membrane temperature, and a behavioral evaluation were recorded before and after interactions with the patient. The results demonstrated that therapy dogs had significantly decreased heart rate and increased heart rate variability after the AAT session. Right tympanic temperature significantly increased after the session, but there was no significant difference in terms of salivary cortisol or oxytocin levels, nor in stress-related behavioral evaluations after the AAT session. Taken together, the results suggest that incorporating AAT into an inpatient stroke rehabilitation program did not induce stress in the therapy dogs, and that the therapy dogs may have been more relaxed after the session. Full article
(This article belongs to the Special Issue The Complexity of the Human–Companion Animal Bond)
15 pages, 6640 KiB  
Article
Evaluation of the Effects of Autologous Leukocyte- and Platelet-Rich Fibrin Membranes for Treating Chronic Wounds: A Prospective Study
by Federica Aragosa, Gerardo Fatone, Chiara Caterino, Stefano Cavalli, Alfonso Piscitelli, Rosario Vallefuoco, Francesco Lamagna and Giovanni Della Valle
Animals 2025, 15(1), 112; https://doi.org/10.3390/ani15010112 - 6 Jan 2025
Viewed by 245
Abstract
(1) Background: The aim of this prospective study was to evaluate the efficacy of topical application of leukocyte- and platelet-rich fibrin (L-PRF) membranes for treating chronic cutaneous wounds in dogs. (2) Methods: Chronic wounds unresponsive to conventional treatments were assessed on digital photographs [...] Read more.
(1) Background: The aim of this prospective study was to evaluate the efficacy of topical application of leukocyte- and platelet-rich fibrin (L-PRF) membranes for treating chronic cutaneous wounds in dogs. (2) Methods: Chronic wounds unresponsive to conventional treatments were assessed on digital photographs and classified using the Bates-Jensen Wound Assessment Tool (BWAT). Each lesion was treated with L-PRF membranes. Epithelialization, granulation tissue formation, wound healing rate, and contraction were statistically analyzed during follow-up. (3) Results: All but one of the cases were treated with a single application of L-PRF for a median of 30 days after onset. All wounds healed after a median of 22 days. The BWAT score, total wound area, and granulation tissue decreased significantly over time while wound contraction increased. The most significant reduction in the total wound area was observed within the first 3 weeks after treatment. None of the dogs required the administration of antibiotics. (4) Conclusions: L-PRF membrane promoted the wound healing process in dogs and is a valuable and cost-effective tool for treating chronic wounds, thereby reducing the need for antibiotics administration. Full article
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<p>Total wound area at T0 (<b>a</b>) and epithelialization area at presentation (<b>b</b>) in Case 6.</p>
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<p>Case 15 after application of the L-PRF membrane.</p>
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<p>Granulation tissue area (GTA) at T1 in Case 6.</p>
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<p>Total wound area (TWA), epithelialization area (EA), and granulation tissue area (GTA) during the follow-up. Values expressed as medians.</p>
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<p>Bates-Jensen Wound Assessment Tool (BWAT) scores during the follow-up.</p>
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<p>Wound contraction and wound healing during follow-up for all dogs.</p>
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<p>Change in total wound area (TWA) as a percentage of TWA at T0 in the individual dogs. The dashed line shows the nonlinear regression curve plotted using the nls function in R software.</p>
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