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Keywords = aptatoxisensor

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Article
Electrochemical Aptatoxisensor Responses on Nanocomposites Containing Electro-Deposited Silver Nanoparticles on Poly(Propyleneimine) Dendrimer for the Detection of Microcystin-LR in Freshwater
by Mawethu P. Bilibana, Avril R. Williams, Candice Rassie, Christopher E. Sunday, Hlamulo Makelane, Lindsay Wilson, Nomaphelo Ntshongontshi, Abongile N. Jijana, Milua Masikini, Priscilla G. L. Baker and Emmanuel I. Iwuoha
Sensors 2016, 16(11), 1901; https://doi.org/10.3390/s16111901 - 11 Nov 2016
Cited by 30 | Viewed by 6514
Abstract
A sensitive and reagentless electrochemical aptatoxisensor was developed on cobalt (II) salicylaldiimine metallodendrimer (SDD–Co(II)) doped with electro-synthesized silver nanoparticles (AgNPs) for microcystin-LR (L, l-leucine; R, l-arginine), or MC-LR, detection in the nanomolar range. The GCE|SDD–Co(II)|AgNPs aptatoxisensor was fabricated with 5’ thiolated [...] Read more.
A sensitive and reagentless electrochemical aptatoxisensor was developed on cobalt (II) salicylaldiimine metallodendrimer (SDD–Co(II)) doped with electro-synthesized silver nanoparticles (AgNPs) for microcystin-LR (L, l-leucine; R, l-arginine), or MC-LR, detection in the nanomolar range. The GCE|SDD–Co(II)|AgNPs aptatoxisensor was fabricated with 5’ thiolated aptamer through self-assembly on the modified surface of the glassy carbon electrode (GCE) and the electronic response was measured using cyclic voltammetry (CV). Specific binding of MC-LR with the aptamer on GCE|SDD–Co(II)|AgNPs aptatoxisensor caused the formation of a complex that resulted in steric hindrance and electrostatic repulsion culminating in variation of the corresponding peak current of the electrochemical probe. The aptatoxisensor showed a linear response for MC-LR between 0.1 and 1.1 µg·L−1 and the calculated limit of detection (LOD) was 0.04 µg·L−1. In the detection of MC-LR in water samples, the aptatoxisensor proved to be highly sensitive and stable, performed well in the presence of interfering analog and was comparable to the conventional analytical techniques. The results demonstrate that the constructed MC-LR aptatoxisensor is a suitable device for routine quantification of MC-LR in freshwater and environmental samples. Full article
(This article belongs to the Special Issue Aptasensors 2016)
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Figure 1
<p>First-generation cobalt(II) salicylaldiimine metallodendrimer (SDD–Co(II)).</p> Full article ">Figure 2
<p>Schematic representation of cobalt(II) salicylaldiimine metallodendrimer aptatoxisensor preparation.</p> Full article ">Figure 3
<p>Cyclic voltammetric traces for: (<b>A</b>) GCE|SDD–Co(II)|(black); (<b>B</b>) GCE|SDD–Co(II)|AgNPs|(red); and (<b>C</b>) GCE|SDD–Co(II)|AgNPs|MCLRA (green) in 0.1 M PBS at a 100 mV·s<sup>−1</sup> scan rate.</p> Full article ">Figure 4
<p>EIS Nyquist plots of the modified electrodes: (<b>A</b>) GCE|SDD–Co(II)|AgNPs; and (<b>B</b>) GCE|SDD–Co(II)|AgNPs|Aptamer, for experiments performed in 0.1 M PBS (pH 7.4).</p> Full article ">Figure 5
<p>FTIR of: (<b>A</b>) GCE|SDD–Co(II); and (<b>B</b>) GCE|SDD–Co(II)|AgNPs.</p> Full article ">Figure 6
<p>(<b>A</b>) Cyclic voltammograms of aptatoxisensor for detection of MC-LR; and (<b>B</b>) calibration curve showing GCE|SDD–Co(II)|AgNPs|MC-LRA responses to MC-LR.</p> Full article ">Figure 7
<p>Relative CV responses used to evaluate cross-reactivity between freshwater toxins. EE2 = 17β-estradiol; NOD = Nodularin-R; ZEO = zearalenone.</p> Full article ">
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