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Search Results (12,910)

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15 pages, 402 KiB  
Review
Advances in Sorghum Improvement for Climate Resilience in the Global Arid and Semi-Arid Tropics: A Review
by Andekelile Mwamahonje, Zamu Mdindikasi, Devotha Mchau, Emmanuel Mwenda, Daines Sanga, Ana Luísa Garcia-Oliveira and Chris O. Ojiewo
Agronomy 2024, 14(12), 3025; https://doi.org/10.3390/agronomy14123025 (registering DOI) - 19 Dec 2024
Abstract
Sorghum is a climate-resilient crop which has been cultivated as a staple food in the semi-arid areas of Africa and Asia for food and nutrition security. However, the current climate change is increasingly affecting sorghum performance, especially at the flowering stage when water [...] Read more.
Sorghum is a climate-resilient crop which has been cultivated as a staple food in the semi-arid areas of Africa and Asia for food and nutrition security. However, the current climate change is increasingly affecting sorghum performance, especially at the flowering stage when water availability is critical for grain filling, thus lowering the sorghum grain yield. The development of climate-resilient, biotic and abiotic stress-tolerant, market-preferred, and nutrient-dense sorghum varieties offers a potentially cost-effective and environmentally sustainable strategy for adapting to climate change. Some of the common technologies for sorghum improvement include mass selection, single seed descent, pure line selection, and marker-assisted selection, facilitated by backcrossing and genotyping using molecular markers. In addition, recent advancements including new machine learning algorithms, gene editing, genomic selection, rapid generation advancement, and recycling of elite material, along with high-throughput phenotyping tools such as drone- and satellite-based images and other speed-breeding techniques, have increased the precision, speed, and accuracy of new crop variety development. In addition to these modern breeding tools and technologies, enhancing genetic diversity to incorporate various climate resilience traits, including against heat and drought stress, into the current sorghum breeding pools is critical. This review covers the potential of sorghum as a staple food crop, explores the genetic diversity of sorghum, discusses the challenges facing sorghum breeding, highlights the recent advancements in technologies for sorghum breeding, and addresses the perceptions of farmers on sorghum production under the current climate change conditions. Full article
(This article belongs to the Section Crop Breeding and Genetics)
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<p>A graphical representation of top 10 sorghum producers in the world.</p>
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20 pages, 5494 KiB  
Article
Real-Time Common Rust Maize Leaf Disease Severity Identification and Pesticide Dose Recommendation Using Deep Neural Network
by Zemzem Mohammed Megersa, Abebe Belay Adege and Faizur Rashid
Knowledge 2024, 4(4), 615-634; https://doi.org/10.3390/knowledge4040032 (registering DOI) - 19 Dec 2024
Abstract
Maize is one of the most widely grown crops in Ethiopia and is a staple crop around the globe; however, common rust maize disease (CRMD) is becoming a serious problem and severely impacts yields. Conventional CRMD detection and treatment methods are time-consuming, expensive, [...] Read more.
Maize is one of the most widely grown crops in Ethiopia and is a staple crop around the globe; however, common rust maize disease (CRMD) is becoming a serious problem and severely impacts yields. Conventional CRMD detection and treatment methods are time-consuming, expensive, and ineffective. To address these challenges, we propose a real-time deep-learning model that provides disease detection and pesticide dosage recommendations. In the model development process, we collected 5000 maize leaf images experimentally, with permission from Haramaya University, and increased the size of the dataset to 8000 through augmentation. We applied image preprocessing techniques such as image equalization, noise removal, and enhancement to improve model performance. Additionally, during training, we utilized batch normalization, dropout, and early stopping to reduce overfitting, improve accuracy, and improve execution time. The optimal model recognizes CRMD and classifies it according to scientifically established severity levels. For pesticide recommendations, the model was integrated with the Gradio interface, which provides real-time recommendations based on the detected disease type and severity. We used a convolutional neural network (CNN), specifically the ResNet50 model, for this purpose. To evaluate its performance, ResNet50 was compared with other state-of-the-art algorithms, including VGG19, VGG16, and AlexNet, using similar parameters. ResNet50 outperformed the other CNN models in terms of accuracy, precision, recall, and F-score, achieving over 97% accuracy in CRMD classification—surpassing the other algorithms by more than 2.5% in both experimental and existing datasets. The agricultural experts verified the accuracy of the recommendation system across different stages of the disease, and the system demonstrated 100% accuracy. Additionally, ResNet50 exhibited lower time complexity during model development. This study demonstrates the potential of ResNet50 models for improving maize disease management. Full article
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<p>The architecture of the proposed system.</p>
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<p>Image segmentation (from the experiment). (<b>A</b>) Original <span class="html-italic">input image.</span> (<b>B</b>) Segmented image.</p>
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<p>Sample images for common rust maize disease [<a href="#B33-knowledge-04-00032" class="html-bibr">33</a>].</p>
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<p>Sample confusion matrix of Resnet50 model without dropout and early stop.</p>
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<p>Training and validation accuracy of different networks (Before optimizations).</p>
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<p>Confusion matrix of different models.</p>
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<p>Confusion matrix of different models.</p>
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<p>Confusion matrix of different models.</p>
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<p>Common rust fungicide dose recommendation prototype.</p>
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16 pages, 706 KiB  
Article
Ecophysiological and Molecular Analysis of Contrasting Genotypes for Leaf Senescence in Sunflower (Helianthus annuus L.) Under Differential Doses of N in Soil
by Daniela E. Becheran, Melanie A. Corzo, Edmundo L. Ploschuk, Salvador Nicosia, Sebastian Moschen, Sofia Bengoa Luoni, Julio Di Rienzo, Nicolas Heinz, Daniel Álvarez and Paula Fernandez
Plants 2024, 13(24), 3540; https://doi.org/10.3390/plants13243540 (registering DOI) - 18 Dec 2024
Abstract
Leaf senescence in plants is the last stage of leaf development and is characterized by a decline in photosynthetic activity, an active degeneration of cellular structures, and the recycling of accumulated nutrients to areas of active growth, such as buds, young leaves, flowers, [...] Read more.
Leaf senescence in plants is the last stage of leaf development and is characterized by a decline in photosynthetic activity, an active degeneration of cellular structures, and the recycling of accumulated nutrients to areas of active growth, such as buds, young leaves, flowers, fruits, and seeds. This process holds economic significance as it can impact yield, influencing the plant’s ability to maintain an active photosynthetic system during prolonged periods, especially during the grain filling stage, which affects plant weight and oil content. It can be associated with different stresses or environmental conditions, manifesting itself widely in the context of climate change and limiting yield, especially in crops of agronomic relevance. In this work, we study the stability of two widely described sunflower (Helianthus annuus L.) genotypes belonging to the INTA Breeding Program against differential N conditions, to verify their yield stability in control conditions and under N supply. Two inbred lines were utilized, namely R453 (early senescence) and B481-6 (late senescence), with contrasting nitrogen availability in the soil but sharing the same ontogeny cycle length. It was observed that, starting from R5.5, the B481-6 genotype not only delayed senescence but also exhibited a positive response to increased nitrogen availability in the soil. This response included an increase in intercepted radiation, resulting in a statistically significant enhancement in grain yield. Conversely, the R453 genotype did not show significant differences under varying nitrogen availability and exhibited a tendency to decrease grain yield when nitrogen availability was increased. The response to nitrogen can vary depending on the specific genotype. Full article
25 pages, 72113 KiB  
Article
Assessing the Sustainability of Miscanthus and Willow as Global Bioenergy Crops: Current and Future Climate Conditions (Part 1)
by Mohamed Abdalla, Astley Hastings, Grant Campbell, Heyu Chen and Pete Smith
Agronomy 2024, 14(12), 3020; https://doi.org/10.3390/agronomy14123020 - 18 Dec 2024
Abstract
Miscanthus (Miscanthus × giganteus) and Willow (Salix spp.) are promising bioenergy crops due to their high biomass yields and adaptability to diverse climatic conditions. This study applies the MiscanFor/SalixFor models to assess the sustainability of these crops under current and [...] Read more.
Miscanthus (Miscanthus × giganteus) and Willow (Salix spp.) are promising bioenergy crops due to their high biomass yields and adaptability to diverse climatic conditions. This study applies the MiscanFor/SalixFor models to assess the sustainability of these crops under current and future climate scenarios, focusing on biomass productivity, carbon intensity (CI), and energy use efficiency (EUE). Under present conditions, both crops show high productivity in tropical and subtropical regions, with Miscanthus generally outperforming Willow. Productivity declines in less favourable climates, emphasising the crops’ sensitivity to environmental factors at the regional scale. The average productivity for Miscanthus and Willow was 19.9 t/ha and 10.4 t/ha, respectively. Future climate scenarios (A1F1, representing world markets and fossil-fuel-intensive, and B1, representing global sustainability) project significant shifts, with northern and central regions becoming more viable for cultivation due to warmer temperatures and extended growing seasons. However, southern and arid regions may experience reduced productivity, reflecting the uneven impacts of climate change. Miscanthus and Willow are predicted to show productivity declines of 15% and 8% and 12% and 7% under A1F1 and B1, respectively. CI analysis reveals substantial spatial variability, with higher values in industrialised and temperate regions due to intensive agricultural practices. Future scenarios indicate increased CI in northern latitudes due to intensified land use, while certain Southern Hemisphere regions may stabilise or reduce CI through mitigation strategies. Under climate change, CI for Miscanthus is projected to increase by over 100%, while Willow shows an increase of 64% and 57% for A1F1 and B1, respectively. EUE patterns suggest that both crops perform optimally in tropical and subtropical climates. Miscanthus shows a slight advantage in EUE, though Willow demonstrates greater adaptability in temperate regions. Climate change is expected to reduce EUE for Miscanthus by 10% and 7% and for Willow by 9% and 6%. This study underscores the need for region-specific strategies to optimise the sustainability of bioenergy crops under changing climate conditions. Full article
(This article belongs to the Special Issue Advances in Grassland Productivity and Sustainability — 2nd Edition)
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<p>Simulated global biomass productivity (t/ha) for Miscanthus (<b>a</b>) and Willow (<b>b</b>) under current climate conditions (1961–1990). For Miscanthus: very low ≤ 5.00; low = 5.00–9.99; medium = 10.00–19.99; high = 20.00–30.00; very high ≥ 30.00. For Willow: very low ≤ 5.00; low = 5.00–9.99; medium = 10.00–14.99; high = 15.00–20.00; very high ≥ 20.00.</p>
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<p>Regional biomass productivity (<b>a</b>), carbon emissions intensity (<b>b</b>), and energy use efficiency (<b>c</b>) of Miscanthus (blue) and Willow (green) at baseline (1961–1990). The error bar is the standard deviation.</p>
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<p>Simulated global biomass productivity (t/ha) for Miscanthus at the A1F1 (<b>a</b>) and B1 (<b>b</b>) climate projections (up to 2060). Very low ≤ 5; low = 5.00–9.99; medium = 10.00–19.99; high = 20.00–30.00; very high ≥ 30.00.</p>
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<p>Simulated global biomass productivity (t/ha) for Miscanthus at the A1F1 (<b>a</b>) and B1 (<b>b</b>) climate projections (up to 2060). Very low ≤ 5; low = 5.00–9.99; medium = 10.00–19.99; high = 20.00–30.00; very high ≥ 30.00.</p>
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<p>Simulated global biomass productivity (t/ha) for Willow at the A1F1 (<b>a</b>) and B1 (<b>b</b>) climate projections (up to 2060). Very low ≤ 5.00; low = 5.00–9.99; medium = 10.00–14.99; high = 15.00–20.00; very high ≥ 20.00.</p>
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<p>Simulated global biomass productivity (t/ha) for Willow at the A1F1 (<b>a</b>) and B1 (<b>b</b>) climate projections (up to 2060). Very low ≤ 5.00; low = 5.00–9.99; medium = 10.00–14.99; high = 15.00–20.00; very high ≥ 20.00.</p>
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<p>Simulated global carbon emissions intensity (gC/MJ) for Miscanthus (<b>a</b>) and Willow (<b>b</b>) under current climate conditions (1961–1990). For Miscanthus and Willow: very low ≤ 0.00; low = 0.00–100.00; medium = 100.01–200.00; high = 200.01–300.00; very high ≥ 300.00.</p>
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<p>Simulated global carbon emissions intensity (gC/MJ) for Miscanthus at the A1F1 (<b>a</b>) and B1 (<b>b</b>) climate projections (up to 2060). Very low ≤ 0.00; low = 0.00–100.00; medium = 100.01–200.00; high = 200.01–300.00; very high ≥ 300.00.</p>
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<p>Simulated global carbon emissions intensity (gC/MJ) for Willow at the A1F1 (<b>a</b>) and B1 (<b>b</b>) climate projections (up to 2060). Very low ≤ 0.00; low = 0.00–100.00; medium = 100.01–200.00; high = 200.01–300.00; very high ≥ 300.00.</p>
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<p>Simulated global energy use efficiency for Miscanthus (<b>a</b>) and Willow (<b>b</b>) under current climate conditions (1961–1990). For Miscanthus: very low ≤ 5; low = 5.00–9.99; medium = 10.00–14.99; high = 15.00–20.00; very high ≥ 20.00. For Willow: very low ≤ 5; low = 5.00–9.99; medium = 10.00–15.00; high ≥ 15.00; very high = not available.</p>
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<p>Simulated global energy use efficiency for Miscanthus (<b>a</b>) and Willow (<b>b</b>) under current climate conditions (1961–1990). For Miscanthus: very low ≤ 5; low = 5.00–9.99; medium = 10.00–14.99; high = 15.00–20.00; very high ≥ 20.00. For Willow: very low ≤ 5; low = 5.00–9.99; medium = 10.00–15.00; high ≥ 15.00; very high = not available.</p>
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<p>Simulated global energy use efficiency for Miscanthus at the A1F1 (<b>a</b>) and B1 (<b>b</b>) climate projections (up to 2060). Very low ≤ 5; low = 5.00–9.99; medium = 10.00–14.99; high = 15.00–20.00; very high ≥ 20.00.</p>
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<p>Simulated global energy use efficiency for Willow at the A1F1 (<b>a</b>) and B1 (<b>b</b>) climate projections (up to 2060). Very low ≤ 5; low = 5.00–9.99; medium = 10.00–15.00; high ≥ 15.00; very high = not available.</p>
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15 pages, 1744 KiB  
Article
Genetic Linkage Map Construction and QTL Mapping for Juvenile Leaf and Growth Traits in Camellia oleifera
by Ling Ye, Yu Li, Yanxuan Liu, Lexin Zhou, Jia’ni Li, Tian Liang, Weiwei Xie, Yiqing Xie, Zhizhen Li, Huanhuan Lv, Na Hou, Gang Wang, Guomin Liu, Guohua Zheng, Shipin Chen and Hui Chen
Agronomy 2024, 14(12), 3022; https://doi.org/10.3390/agronomy14123022 - 18 Dec 2024
Abstract
Advancement of the oil tea industry requires the development of high-yielding and superior-quality varieties of Camellia oleifera, a major oilseed crop. However, traditional breeding methods, hampered by lengthy cycles and low selection accuracy, significantly constrain the breeding process. Identifying single nucleotide polymorphisms [...] Read more.
Advancement of the oil tea industry requires the development of high-yielding and superior-quality varieties of Camellia oleifera, a major oilseed crop. However, traditional breeding methods, hampered by lengthy cycles and low selection accuracy, significantly constrain the breeding process. Identifying single nucleotide polymorphisms (SNPs) associated with target traits, and applying molecular marker-assisted selection (MAS) for these traits, can thereby shorten the breeding cycles and amplify the breeding efficiency. In this study, we utilized the hexaploid C. oleifera as the reference genome to identify high-quality SNPs and constructed a high-density genetic linkage map of C. oleifera that spanned 1566.733 cM, included 3097 SNPs, and was anchored to 15 linkage groups. Using interval mapping, we localized quantitative trait loci (QTLs) for 11 juvenile traits in C. oleifera, identifying 15 QTLs for growth traits and 24 QTLs for leaf traits, including 4 stable QTLs. The logarithm of odds (LOD) scores for individual QTLs ranged from 3.48 to 14.62, explaining 9.86–48.61% of the phenotypic variance. We further identified 2 SNPs associated with growth traits (marker11-951 and marker12-68) and 10 SNPs associated with leaf traits (marker11-276, marker11-410, marker11-560, marker13-16, marker13-39, marker13-110, marker13-731, marker14-701, marker14-910, and marker14-1331). These results provide valuable insights into the genetic mapping of key traits in C. oleifera and will contribute to the development of new varieties with high yield and superior quality in the future. Full article
(This article belongs to the Section Crop Breeding and Genetics)
20 pages, 7094 KiB  
Article
Comparative Analysis of Japanese Quince Juice Concentrate as a Substitute for Lemon Juice Concentrate: Functional Applications as a Sweetener, Acidifier, Stabilizer, and Flavoring Agent
by Vitalijs Radenkovs, Inta Krasnova, Ingmars Cinkmanis, Karina Juhnevica-Radenkova, Edgars Rubauskis and Dalija Seglina
Horticulturae 2024, 10(12), 1362; https://doi.org/10.3390/horticulturae10121362 - 18 Dec 2024
Abstract
This research examined the viability of Japanese quince juice concentrate (JQJC) as an innovative alternative to lemon juice concentrate (LJC). Given the rising consumer demand for natural food ingredients, this study focused on a thorough analysis of the nutritional and functional characteristics of [...] Read more.
This research examined the viability of Japanese quince juice concentrate (JQJC) as an innovative alternative to lemon juice concentrate (LJC). Given the rising consumer demand for natural food ingredients, this study focused on a thorough analysis of the nutritional and functional characteristics of JQJC in comparison to LJC. The chemical analysis indicated that JQJC possesses a total soluble solids (TSS) content of 50.6 °Brix, with fructose and glucose, to a greater extent, being the primary contributors to its solids content. In contrast, LJC had a TSS of 39.8 °Brix and also contained glucose and fructose. Additionally, malic acid is a principal component of JQJC’s acidity, determined at 20.98 g 100 g−1 of fresh weight (FW), while LJC mostly contained citric acid at a concentration of 30.86 g 100 g−1 FW. Moreover, the ascorbic acid content quantified in JQJC was eight times greater than that observed in LJC. The assessment of antioxidant activity, utilizing the DPPH and FRAP assays, indicated that JQJC exhibits scavenging activity nearly eleven times higher than that of LJC, suggesting its superior antioxidant capacity. The total phenolic content for JQJC was quantified at 2189.59 mg 100 g−1 FW, significantly (p < 0.05) exceeding the 262.80 mg 100 g−1 FW found in LJC. The analysis identified 16 individual phenolic compounds in JQJC, highlighting the dominance of epicatechin, chlorogenic, and protocatechuic acids with concentrations ranging from 0.16 to 50.63 mg 100 g−1 FW, contributing to a total individual phenolic content of 114.07 mg 100 g−1 FW. Conversely, LJC is characterized by substantial contributions from hesperidin, eriocitrin, and, to a lesser extent, quercetin-3-O-rutinoside, yielding a phenolic content of 109.65 mg 100 g−1 FW. This study presents strong evidence supporting the utilization of JQJC as a functional substitute for LJC across a variety of product categories, including beverages, jams, and other food items. The findings indicate that JQJC has the potential to enhance product development targeted at health-conscious consumers while optimizing the utilization of a relatively underexplored fruit crop. Full article
(This article belongs to the Section Processed Horticultural Products)
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<p>Fruit of the Japanese quince (<span class="html-italic">Chaenomeles japonica</span> L.) utilized in the production of juice concentrate.</p>
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<p>Extracted ion chromatogram (EIC) in multiple reaction monitoring (MRM) mode represents the profile of 21 phenolic standards at a concentration of 0.6 μg mL<sup>−1</sup>. Note: 1—Gallic acid; 2—Neochlorogenic acid; 3—Protocatechuic acid; 4—Chlorogenic acid; 5—(+)-Catechin; 6—(-)-Epicatechin; 7—Caffeic acid; 8—Myricetin-3-<span class="html-italic">O</span>-glucoside; 9—Quercetin-3-<span class="html-italic">O</span>-rutinoside (rutin); 10—Luteolin-7-<span class="html-italic">O</span>-glucoside (cynaroside); 11—Quercetin-3-<span class="html-italic">O</span>-galactoside (hyperoside); 12—Quercetin-3-<span class="html-italic">β</span>-glucoside (isoquercitrin); 13—Myricetin-3-<span class="html-italic">O</span>-rhamnoside (myricitrin); 14—Kaempferol-3-<span class="html-italic">O</span>-rutinoside (nicotiflorin); 15—Quercetin-3-<span class="html-italic">O</span>-rhamnoside (quercitrin); 16—Myricetin (aglycone); 17—Luteolin (aglycone); 18—Quercetin; 19—Kaempferol; 20—Rhamnetin; 21—Isorhamnetin.</p>
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<p>A representative profile of saccharides detected in Japanese quince (<b>A</b>) and lemon juice (<b>B</b>) concentrates. Sample injection volume of 15 µL, corresponding to a concentration of 0.075 µg mL<sup>−1</sup>. Note: 1—Glycerol; 2—Ribose; 3—Xylose; 4—Arabinose; 5—Fructose; 6—Mannose; 7—Glucose; 8—Sorbitol; 9—Galactose; 10—Sucrose; 11—Maltose; 12—Lactose. Unknown peaks 1, 2, 3, and 4 correspond to unidentified compounds in Japanese quince and lemon juice concentrates.</p>
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<p>Extracted ion chromatogram (EIC) in multiple reaction monitoring mode represents the profile of major phenolic compounds detected in Japanese quince juice concentrate. Note: 1—Gallic acid; 2—Neochlorogenic acid; 3—Protocatechuic acid; 4—Chlorogenic acid; 5—(+)-Catechin; 6—(-)-Epicatechin; 7—Caffeic acid; 9—Quercetin-3-<span class="html-italic">O</span>-rutinoside (rutin); 11—Quercetin-3-<span class="html-italic">O</span>-galactoside (hyperoside); 12—Quercetin-3-<span class="html-italic">β</span>-glucoside (isoquercitrin); 15—Quercetin-3-<span class="html-italic">O</span>-rhamnoside (quercitrin); 18—Quercetin.</p>
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<p>Extracted ion chromatogram (EIC) in multiple reaction monitoring mode represents the profile of major phenolic compounds detected in lemon juice concentrate. Note: 9—Quercetin-3-<span class="html-italic">O</span>-rutinoside (rutin); 18—Quercetin.</p>
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16 pages, 3067 KiB  
Article
Field Application of Mycorrhizal Inoculant Influences Growth, Nutrition, and Physiological Parameters of Corn Plants and Affects Soil Microbiological Attributes
by Paulo Ademar Avelar Ferreira, Carina Marchezan, Gustavo Scopel, Natalia Teixeira Schwab, Emanuela Pille da Silva, Cláudio Roberto Fonsêca Sousa Soares, Gustavo Brunetto and Sidney Luiz Stürmer
Agronomy 2024, 14(12), 3006; https://doi.org/10.3390/agronomy14123006 - 17 Dec 2024
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Abstract
Mycorrhizal inoculants can contribute to the development of corn crops by improving crop productivity. In this sense, the objective of this study was to evaluate the effects of a mycorrhizal inoculant on the dynamics of root system growth, gas exchange, corn crop productivity, [...] Read more.
Mycorrhizal inoculants can contribute to the development of corn crops by improving crop productivity. In this sense, the objective of this study was to evaluate the effects of a mycorrhizal inoculant on the dynamics of root system growth, gas exchange, corn crop productivity, and microbial activity in the rhizospheric soil in a no-till area with different levels of available soil phosphorus. The experiment was conducted during the 2019/2020 and 2020/2021 growing seasons. At 75 days after plant emergence, root morphological parameters (total root length (cm), average root diameter (mm), root surface area (cm2), and root volume), shoot biomass production, P content in the plant shoots, gas exchange, and microbiological attributes of the rhizospheric soil of corn were evaluated. At the end of the cycle, corn grain yield was determined. A beneficial effect of AMF inoculation was observed on the root and shoot parameters regardless of soil P level. Under conditions of evenly distributed rainfall during the experiment (2019/2020 season), AMF inoculation contributed to a 90% increase in acid phosphatase activity and a 76% increase in microbial biomass carbon (C-BIO), independent of soil P level. In contrast, under water deficit conditions (2020/2021 season), AMF inoculation provided a 29% increase in grain yield. We concluded that introducing a commercial mycorrhizal inoculant in corn benefits root system morphological parameters and physiological traits, and favors the activity of enzymes related to increased P availability, contributing to increased crop productivity in a no-till system. Full article
(This article belongs to the Special Issue Microorganisms in Agriculture—Nutrition and Health of Plants)
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<p>Corn sowing dates, precipitation, minimum, average, and maximum air temperatures throughout the 2019/2020 and 2020/2021 growing seasons. Santa Maria, RS, Brazil.</p>
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<p>Total length (<b>a</b>), average diameter (<b>b</b>), surface area (<b>c</b>), and root volume (<b>d</b>) of corn in the 2019/20 and 2020/21 cropping seasons in a no-till area after successive applications of phosphorus fertilizer with and without AMF inoculation. Means followed by the same uppercase letters compare inoculation within the same soil P level, and lowercase letters compare soil P levels under the same inoculation condition (Tukey—<span class="html-italic">p</span> ≤ 0.05). In figures (<b>a</b>,<b>d</b>), the orange bars compare the effect of inoculation when the interaction between the factors was not significant. In figure (<b>b</b>) the orange bars compare the P levels in the soil when the interaction between the factors was not significant.</p>
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<p>Distribution of the average root diameter observed in the 0–60 cm soil layer in corn plants grown under low and high soil P conditions, inoculated and non-inoculated with AMF in the (<b>a</b>) 2019/20 and (<b>b</b>) 2020/21 cropping seasons. The diameters evaluated were &lt;0.25, 0.25-0.40, 0.40-0.55 and greater than 0.55 mm.</p>
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<p>Dry matter production (<b>a</b>), P content in the shoot (<b>b</b>), and grain yield (<b>c</b>) of corn in the 2019/20 and 2020/21 cropping seasons in a no-till area after successive applications of phosphorus fertilizer with and without AMF inoculation. Means followed by the same uppercase letters compare inoculation within the same soil P level, and lowercase letters compare soil P levels under the same inoculation condition (Tukey—<span class="html-italic">p</span> ≤ 0.05). The oranges compare the effect of soil P levels when the interaction between the factors was not significant.</p>
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<p>Net photosynthesis rate (<b>a</b>), Intercellular CO<sub>2</sub> concentration (<b>b</b>), Instantaneous carboxylation efficiency (A/Ci) (<b>c</b>), and water use efficiency (WUE) (<b>d</b>) of corn in the 2019/20 and 2020/21 cropping seasons in a no-till area after successive applications of phosphorus fertilizer with and without AMF inoculation. Means followed by the same uppercase letters compare inoculation within the same soil P level, and lowercase letters compare soil P levels under the same inoculation condition (Tukey—<span class="html-italic">p</span> ≤ 0.05). The oranges compare the effect of inoculation when the interaction between the factors was not significant.</p>
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<p>Activity of the enzymes acid phosphatase (<b>a</b>) and β-glucosidase (<b>b</b>) in the rhizospheric soil of corn in a no-till area after successive applications of phosphorus fertilizer with and without AMF inoculation. Means followed by the same uppercase letters compare inoculation within the same soil P level, and lowercase letters compare soil P levels under the same inoculation condition (Tukey—<span class="html-italic">p</span> ≤ 0.05).</p>
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16 pages, 1394 KiB  
Article
Effects of Seven-Year-Optimized Irrigation and Nitrogen Management on Dynamics of Soil Organic Nitrogen Fractions, Soil Properties, and Crop Growth in Greenhouse Production
by Jianshuo Shi, Longgang Jiang, Liying Wang, Chengzhang Wang, Ruonan Li, Lijia Pan, Tianyuan Jia, Shenglin Hou and Zhou Jia
Agriculture 2024, 14(12), 2319; https://doi.org/10.3390/agriculture14122319 - 17 Dec 2024
Viewed by 254
Abstract
Exploring the temporal evolution dynamics of different soil organic nitrogen (N) components under different water–N management practices is a useful approach to accurately assessing N supply and soil fertility. This information can provide a scientific basis for precise water and N management methods [...] Read more.
Exploring the temporal evolution dynamics of different soil organic nitrogen (N) components under different water–N management practices is a useful approach to accurately assessing N supply and soil fertility. This information can provide a scientific basis for precise water and N management methods for greenhouse vegetable production. The objective of this study was to investigate the effects of optimized irrigation and nitrogen management on the dynamics of soil organic nitrogen fractions, soil properties, and crop growth. This research was conducted from 2017 to 2023 in a greenhouse vegetable field in North China. Four treatments were applied: (1) high chemical N application with furrow irrigation (farmers’ practice, FP); (2) no chemical N application with drip irrigation (DN0); (3) 50% N of FP with drip irrigation (DN1); and (4) 75% N of FP with drip irrigation (DN2). The volume in drip irrigation is 70% of that in furrow irrigation. The results showed that in 2023 (after seven years of field trials), compared with FP, the soil organic carbon (SOC), total N, and water use efficiency of the DN1 and DN2 treatments increased by 15.9%, 11.4%, and 11.3% and 7.7%, 47.2% and 44.6%, respectively. However, there was no significant difference in the total crop yield except in the DN0 treatment. Soil organic N was mostly in the form of acid-hydrolyzed N (AHN). After seven years of optimized irrigation and N management, the DN1 treatment significantly increased the content of ammonium N (AN) and amino sugar N (ASN) in AHN compared with the FP treatment. The results of further analysis demonstrated that SOC was the main factor in regulating AHN and non-hydrolyzable N (NHN), while the main regulatory factors for amino acid N (AAN) and ASN in the AHN component were dry biomass and water use efficiency, respectively. From a time scale perspective, optimization of the water and N scheduling, especially in DN1 (reducing the total irrigation volume by 30% and the amount of N applied by 50%), is crucial for the sustainable improvement of soil fertility and the maintenance of vegetable production. Full article
(This article belongs to the Section Agricultural Soils)
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<p>TN (<b>a</b>), SOC (<b>b</b>), C/N (<b>c</b>), NO<sub>3</sub><sup>−</sup>-N (<b>d</b>), pH (<b>e</b>), and EC (<b>f</b>) under various irrigation and N application rates in 2017, 2019, 2021, and 2023. Different lowercase letters mean significant differences among treatments in the same year, and different uppercase letters mean significant differences among different years of the same treatment (<span class="html-italic">p</span> &lt; 0.05). Vertical bars represent standard error of mean. DN0: no chemical N application with drip irrigation; DN1: 50% N of FP with drip irrigation; DN2: 75% N of FP with drip irrigation. TN: soil total N; SOC: soil organic carbon; C/N: the ratio of soil organic C to soil total N; NO<sub>3</sub><sup>−</sup>-N: nitrate nitrogen; pH: soil pH; EC: soil electrical conductivity.</p>
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<p>AN content (<b>a</b>), AAN content (<b>b</b>), ASN content (<b>c</b>), UN content (<b>d</b>), AHN content (<b>e</b>), and NHN content (<b>f</b>) under various irrigation and N application rates in 2017, 2019, 2021, and 2023. Different lowercase letters mean significant differences among treatments in the same year, and different uppercase letters mean significant differences among different years of the same treatment (<span class="html-italic">p</span> &lt; 0.05). Vertical bars represent standard error of mean. DN0: no chemical N application with drip irrigation; DN1: 50% N of FP with drip irrigation; DN2: 75% N of FP with drip irrigation. AN: ammonium N; AAN: amino acid N; ASN: amino sugar N; UN: hydrolyzable unknown N; AHN: acid hydrolyzed N; NHN: non-hydrolyzable N.</p>
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<p>Percentage of soil organic N fraction contents in TN in (<b>a</b>) 2017, (<b>b</b>) 2019, (<b>c</b>) 2021, and (<b>d</b>) 2023 under various irrigation and N application rates. AN: ammonium N; AAN: amino acid N; ASN: amino sugar N; UN: hydrolyzable unknown N; NHN: non-hydrolyzable N. DN0: no chemical N application with drip irrigation; DN1: 50% N of FP with drip irrigation; DN2: 75% N of FP with drip irrigation.</p>
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<p>The relative importance drivers of AN (<b>a</b>), AAN (<b>b</b>), UN (<b>c</b>), ASN (<b>d</b>), AHN (<b>e</b>), and NHN (<b>f</b>). The vertical bars represent 95% confidence intervals. SOC: soil organic carbon; TP: soil total phosphorus; EC: soil electrical conductivity; TK: soil total potassium; TDM: total dry biomass; AK: soil available potassium; TY: total yield; NO<sub>3</sub><sup>−</sup>-N: nitrate nitrogen; annual WUE: annual water use efficiency.</p>
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24 pages, 6615 KiB  
Article
The Identification of AMT Family Genes and Their Expression, Function, and Regulation in Chenopodium quinoa
by Xiangxiang Wang, He Wu, Nazer Manzoor, Wenhua Dongcheng, Youbo Su, Zhengjie Liu, Chun Lin and Zichao Mao
Plants 2024, 13(24), 3524; https://doi.org/10.3390/plants13243524 - 17 Dec 2024
Viewed by 278
Abstract
Quinoa (Chenopodium quinoa) is an Andean allotetraploid pseudocereal crop with higher protein content and balanced amino acid composition in the seeds. Ammonium (NH4+), a direct source of organic nitrogen assimilation, mainly transported by specific transmembrane ammonium transporters ( [...] Read more.
Quinoa (Chenopodium quinoa) is an Andean allotetraploid pseudocereal crop with higher protein content and balanced amino acid composition in the seeds. Ammonium (NH4+), a direct source of organic nitrogen assimilation, mainly transported by specific transmembrane ammonium transporters (AMTs), plays important roles in the development, yield, and quality of crops. Many AMTs and their functions have been identified in major crops; however, no systematic analyses of AMTs and their regulatory networks, which is important to increase the yield and protein accumulation in the seeds of quinoa, have been performed to date. In this study, the CqAMTs were identified, followed by the quantification of the gene expression, while the regulatory networks were predicted based on weighted gene co-expression network analysis (WGCNA), with the putative transcriptional factors (TFs) having binding sites on the promoters of CqAMTs, nitrate transporters (CqNRTs), and glutamine-synthases (CqGSs), as well as the putative TF expression being correlated with the phenotypes and activities of GSs, glutamate synthase (GOGAT), nitrite reductase (NiR), and nitrate reductase (NR) of quinoa roots. The results showed a total of 12 members of the CqAMT family with varying expressions in different organs and in the same organs at different developmental stages. Complementation expression analyses in the triple mep1/2/3 mutant of yeast showed that except for CqAMT2.2b, 11/12 CqAMTs restored the uptake of NH4+ in the host yeast. CqAMT1.2a was found to mainly locate on the cell membrane, while TFs (e.g., CqNLPs, CqG2Ls, B3 TFs, CqbHLHs, CqZFs, CqMYBs, CqNF-YA/YB/YC, CqNACs, and CqWRKY) were predicted to be predominantly involved in the regulation, transportation, and assimilation of nitrogen. These results provide the functions of CqAMTs and their possible regulatory networks, which will lead to improved nitrogen use efficiency (NUE) in quinoa as well as other major crops. Full article
(This article belongs to the Section Plant Genetics, Genomics and Biotechnology)
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<p>Phylogenetic tree of <span class="html-italic">AMTs</span> and their colinear relationship among <span class="html-italic">C. watsonii</span>, <span class="html-italic">C. suecicum</span>, and <span class="html-italic">C. quinoa</span>: (<b>A</b>) Phylogenetic tree of the AMT proteins of six plant species (<span class="html-italic">A. thaliana</span>, <span class="html-italic">O. sativa</span>, <span class="html-italic">S. lycopersicum</span>, <span class="html-italic">P. richocarpa</span>, <span class="html-italic">C. sinensis</span> var. sinensis, and <span class="html-italic">C. quinoa</span>). Each group is represented by a different color, and the CqAMT proteins are marked with red dots. (<b>B</b>) Reservation and loss of the <span class="html-italic">AMT</span> genes among <span class="html-italic">C. watsonii</span> (<span class="html-italic">Cw</span>), <span class="html-italic">C. suecicum</span> (<span class="html-italic">Cs</span>), and <span class="html-italic">C. quinoa</span> (<span class="html-italic">Cq</span>).</p>
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<p>Phylogenetic relationships, conserved motifs, and gene structure analysis of <span class="html-italic">CqAMT</span> genes: (<b>A</b>) Phylogenetic tree of the 12 CqAMT proteins. (<b>B</b>) The conserved protein motifs were identified using MEME; each color represents a motif. The lengths of the motifs are proportional. (<b>C</b>) The exon–intron distribution of <span class="html-italic">CqAMTs</span> with black lines indicated introns, while exons are indicated with yellow boxes (CDS) and blue boxes (UTR).</p>
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<p>Predicted cis-elements in 12 <span class="html-italic">CqAMTs</span> promoters, predicted using PlantCARE.</p>
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<p>Expression patterns of <span class="html-italic">CqAMT</span> genes: (<b>A</b>) W32 leaves and roots under 0, 8, and 21 mM NH<sub>4</sub><sup>+</sup> concentrations, respectively (L-0mM-21D—leaf samples were treated with 0 mM ammonium nitrogen for 21 d, with similar descriptions as those below for L-0mM-27D, L-21mM-21D, L-21mM-27D, L-8mM-21D, and L-8mM-27D; R-0mM-21D—root samples were treated with 0 mM ammonium nitrogen for 21 d, with similar descriptions as those below for R-0mM-27D, R-21mM-21D, R-21mM-27D, R-8mM-21D, and R-8mM-27D). (<b>B</b>) <span class="html-italic">CqAMTs</span> expressed in different developmental reproductive stages of both W19 and W25 planted in field (W19-FL—leaves of W19 at the flower development stage; W19-SL—leaves of W19 at the seed-filling stage; W19-FP—panicles of W19 at the flowering stage; W19-SP—panicles of W19 at the seed formation stage); W25 samples were labeled similarly as W19 samples.</p>
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<p>qRT-PCR analysis of the 10 <span class="html-italic">CqAMT</span>s in both leaves and roots under hydroponic cultivation of W32 after 21 d with 0, 8, and 21 mM NH<sub>4</sub><sup>+</sup> concentrations.</p>
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<p>Functional verification of 11 <span class="html-italic">CqAMTs</span> and <span class="html-italic">CqAMT1.2a</span> subcellular location detection: (<b>A</b>) Growth of the yeast mutants (31019b) was complemented via heterologous expression of <span class="html-italic">CqAMTs</span>. The yeast mutant strain (31019b) was transformed with the empty vector pYES2, or 11 <span class="html-italic">CqAMTs</span> expression vectors, namely CqAMT2.2a-pYES2, CqAMT1.3a-pYES2, CqaMT1.4a-pYES2, CqAMT3.1b-pYES2, CqAMT1.2c-pYES2, CqAMT1.2a-pYES2, CqAMT1.4b-pYES2, CqAMT1.2b-pYES2, CqAMT1.2d-pYES2, CqAMT3.1a-pYES2, and CqAMT1.3b-pYES2. The mutant 31019b transformed with pYES2 was used as a negative control. The transformants were grown on the SD medium at 30 °C for 2–3 days. (<b>B</b>) Subcellular localization detection of <span class="html-italic">CqAMT1.2a</span> was performed by fusing the expression with <span class="html-italic">GFP</span> in tobacco leaves.</p>
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<p>Co-expression network construction and identification of TFs: (<b>A</b>) The expression levels of screened TFs and genes related to nitrogen metabolism in different tissues and different nitrogen concentration samples in BGM. (<b>B</b>) The correlation between physiological traits and the expression level of screened TFs in the BGM. (<b>C</b>) The expression levels of screened TFs and genes related to nitrogen metabolism in different tissues and samples using different nitrogen concentrations in TGM. (<b>D</b>) The correlation between physiological traits and the expression level of screened TFs in TGM. (<b>E</b>) Co-expression network of top 15 TFs in BGM. (<b>F</b>) Co-expression network of top 21 TFs in TGM. (<b>G</b>) TF-TF co-expression network of BGM. (<b>H</b>) TF-TF co-expression network of TGM. The * symbol represents 0.01 &lt; <span class="html-italic">p</span> &lt; 0.05. The ** symbol represents <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Nitrogen uptake and utilization mechanism in <span class="html-italic">Chenopodium quinoa</span> [<a href="#B39-plants-13-03524" class="html-bibr">39</a>,<a href="#B40-plants-13-03524" class="html-bibr">40</a>,<a href="#B41-plants-13-03524" class="html-bibr">41</a>]. NO<sub>3</sub><sup>−</sup>—nitrate; NO<sub>2</sub><sup>−</sup>—nitrite ion; NH<sub>4</sub><sup>+</sup>—ammonium; NRT—nitrate transporter; NiR—nitrite reductase; NR—nitrate reductase; Gln—glutamine; Glu—glutamic acid; GS—glutamine synthase; GOGAT—glutamate synthetase; GDH—glutamate dehydrogenase; α-OG—α-ketoglutaric acid; NADP—nicotinamide adenine dinucleotide phosphate.</p>
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20 pages, 3580 KiB  
Article
Explainable Machine Learning to Map the Impact of Weather and Soil on Wheat Yield and Revenue Across the Eastern Australian Grain Belt
by Patrick Filippi, Brett M. Whelan and Thomas F. A. Bishop
Agriculture 2024, 14(12), 2318; https://doi.org/10.3390/agriculture14122318 - 17 Dec 2024
Viewed by 214
Abstract
Understanding the causes of spatiotemporal variation in crop yields across large areas is important in closing yield gaps and producing more food for the growing global population. While there has been much focus on using data-driven models to predict crop yield, there is [...] Read more.
Understanding the causes of spatiotemporal variation in crop yields across large areas is important in closing yield gaps and producing more food for the growing global population. While there has been much focus on using data-driven models to predict crop yield, there is also an opportunity to use these empirical models to understand which factors are driving variations in yield and to quantify their contributions. This study uses a large database of 625 rainfed wheat yield maps from 14 different seasons (2007–2020) across the eastern grain belt of Australia. XGBoost models were used, with predictors including maps of soil attributes (e.g., pH and sodicity), along with weather indices (rainfall, frost, heat, growing degree days). The model and predictors could accurately predict field-scale yield, with a Lin’s concordance correlation coefficient (LCCC) of 0.78 with 10-fold cross-validation. SHapley Additive exPlanation (SHAP), a form of interpretive machine learning (IML), values were then used to assess the impact of the variables on yield. The SHAP values for each predictor were also mapped onto a grid of the study area for the 2020 season, which showed the impact of each predictor on wheat yield (t ha−1) and revenue (AUD ($) ha−1) in interpretable units. Weather variables, such as rainfall and heat events, had the largest impact on yield. Although generally less significant, soil constraints such as soil sodicity were still important in driving yield. The results also showed that despite their largely temporally stable nature, soil constraints impact yield differently, depending on seasonal conditions. Overall, data-driven models and IML proved valuable in understanding the impact of important weather and soil variables on wheat yield and revenue across the eastern Australian grain belt. This could be used to determine the magnitude and economic impact of soil constraints and extreme weather on crops across regions and to inform policies and farm management decisions. Full article
(This article belongs to the Section Digital Agriculture)
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<p>The grain belt (red outline) in the Murray–Darling Basin (white outline) and locations of yield data (yellow points).</p>
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<p>Number of wheat yield maps used for modelling in each cropping season.</p>
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<p>Pearson correlation coefficients (r) between wheat yield and the spatial covariates used for modelling. “pre_rain” = pre-season rainfall; “early_rain” = early-season rainfall; “late_rain” = late-season rainfall.</p>
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<p>Observed and predicted yield (t ha<sup>−1</sup>) from the 10-fold cross-validation. Red dotted line represents the 1:1 line.</p>
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<p>SHAP summary plots from the XGBoost yield model. The position on the x-axis is determined by the SHAP value, which represents the feature effect on yield for each data point in t ha<sup>−1</sup>. The colour indicates the feature value from low to high. The position on the y-axis is ordered by the decreasing mean absolute SHAP value for each feature. “pre_rain” = pre-season rainfall; “early_rain” = early-season rainfall; “late_rain” = late-season rainfall.</p>
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<p>Boxplot of estimated impact of predictor variables on revenue (AUD (<span>$</span>) ha<sup>−1</sup>) and yield (t ha<sup>−1</sup>) in the 2020 season across the study area. “pre_rain” = pre-season rainfall; “early_rain” = early-season rainfall; “late_rain” = late-season rainfall.</p>
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<p>Maps of the most important rainfall (late-season), temperature (heat days), and soil (subsoil exchangeable sodium percentage (ESP)) predictor variables and their corresponding impact on yield (SHAP value) for the 2020 season.</p>
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<p>Maps of impact of soil exchangeable sodium percentage (ESP) for the 30–60 cm layer on yield (SHAP value) for a ‘good’ (2020), ‘poor’ (2019), and ‘average (2015) rainfall season.</p>
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<p>The most limiting variables impacting wheat yield across the eastern Australian grain belt in the 2020 season.</p>
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17 pages, 5855 KiB  
Article
Effects of Exogenous Organic Matter on Soil Nutrient Dynamics and Its Role in Replacing Chemical Fertilizers for Vegetable Yield and Quality
by Juan Wang, Xinyue Li, Anquan Chen, Yan Li, Mengyun Xue and Shaoyuan Feng
Horticulturae 2024, 10(12), 1355; https://doi.org/10.3390/horticulturae10121355 - 17 Dec 2024
Viewed by 221
Abstract
Searching for a low-cost soil amendment that can reduce the reliance on chemical fertilizers while maintaining crop yields is a vital issue to sustainable agricultural development. In this study, bio-organic matter derived from harmless disposal of livestock and poultry carcasses was used to [...] Read more.
Searching for a low-cost soil amendment that can reduce the reliance on chemical fertilizers while maintaining crop yields is a vital issue to sustainable agricultural development. In this study, bio-organic matter derived from harmless disposal of livestock and poultry carcasses was used to discuss its potential for substitute chemical fertilizer. An incubation experiment was conducted by incorporating bio-organic matter into the soil at the rate of 0% (CK), 1%, 2%, 3%, 4%, 5%, 6% and 7% (T1, T2, T3, T4, T5, T6, T7) of soil mass to investigate the effects of bio-organic matter on soil physical properties and the nutrient release dynamics. A pot experiment was conducted with three treatments: 150 mg·kg−1 nitrogen from compound fertilizer (CK), 150 mg·kg−1 nitrogen from bio-organic matter (B1) and 300 mg·kg−1 nitrogen from bio-organic matter (B2), to evaluate the potential of bio-organic matter as a substitute for chemical fertilizers in influencing the yield and quality of Chinese cabbage (Brassica chinensis L.). Results showed that in the incubation experiment, bio-organic matter addition reduced soil bulk density of 1.5% to 8.9% and increased soil porosity by ranging from 1.5% to 10.9%. The soil physical properties were significantly improved when addition rates ≥ 4% (by soil mass). The interaction effects of addition rate and incubation time had a significant effect on soil nutrients. In the pot experiment, substitution of chemical fertilizer with bio-organic matter did not reduce the yield, and the increasing application rate of bio-organic matter led to significantly higher soluble protein, soluble sugar and total phenol content of vegetables. Additionally, nitrite content in the vegetables was slightly lower with bio-organic matter compared to that under CK. It is concluded that bio-organic matter derived from the harmless disposal of livestock and poultry carcasses is feasible; it has great potential to partially or entirely replace chemical fertilizers, thereby contributing to realizing chemical fertilizer reduction. Full article
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<p>Soil bulk density and soil porosity under different treatments. Notes: The different lowercase letters indicate significant difference (at <span class="html-italic">p</span> &lt; 0.05) according to Duncan’s test, the same as below. Notes: CK, T1, T2, T3, T4, T5, T6 and T7 represent the treatments with bio-organic matter addition rate at 0%, 1%, 2%, 3%, 4%, 5%, 6%, and 7% (by soil mass), respectively, the same as below. The errors bars shown in the figures are standard error, the same as below.</p>
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<p>Soil available nitrogen content (mg·kg<sup>−1</sup>) under different treatments in three periods. Notes: Different uppercase letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) in soil available nitrogen content at various sampling times under the same addition rate according to Duncan’s test, while lowercase letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) in soil available nitrogen content across different addition rates at the same time according to Duncan’s test. ** means extremely significant at <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Soil available phosphorus content (mg·kg<sup>−1</sup>) under different treatments in three periods. Notes: Different uppercase letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) in soil available phosphorus content at various sampling times under the same addition rate according to Duncan’s test, while lowercase letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) in soil available phosphorus content across different addition rates at the same time according to Duncan’s test. ** means extremely significant at <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Soil available potassium content (mg·kg<sup>−1</sup>) under different treatments in three periods. Notes: Different uppercase letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) in soil available potassium content at various sampling times under the same addition rate according to Duncan’s test, while lowercase letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) in soil available potassium content across different addition rates at the same time according to Duncan’s test. ** means extremely significant at <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>Soil organic matter content (g·kg<sup>−1</sup>) under different treatments in three periods. Notes: Different uppercase letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) in soil organic matter content at various sampling times under the same addition rate according to Duncan’s test, while lowercase letters indicate significant differences (<span class="html-italic">p</span> &lt; 0.05) in soil organic matter content across different addition rates at the same time according to Duncan’s test. NS means not significant (<span class="html-italic">p</span> &gt; 0.05). ** means extremely significant at <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>The soil temperature dynamics during the growth period. Notes: CK represents compound fertilizer with equal nitrogen application of 150 mg·kg<sup>−1</sup>, and B1 and B2 represent bio-organic matter with equal nitrogen application of 150 mg·kg<sup>−1</sup> and 300 mg·kg<sup>−1</sup>, respectively, the same as below.</p>
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<p>Accumulative irrigation water amount under different treatments.</p>
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<p>Correlation analysis of soil nutrients with yield and quality indices. Notes: * means significant at <span class="html-italic">p</span> &lt; 0.05.</p>
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15 pages, 2787 KiB  
Article
Comparative Salt-Stress Responses in Salt-Tolerant (Vikinga) and Salt-Sensitive (Regalona) Quinoa Varieties. Physiological, Anatomical and Biochemical Perspectives
by Xavier Serrat, Antony Quello, Brigen Manikan, Gladys Lino and Salvador Nogués
Agronomy 2024, 14(12), 3003; https://doi.org/10.3390/agronomy14123003 - 17 Dec 2024
Viewed by 321
Abstract
Soil salinization is an important stress factor that limits plant growth and yield. Increased salinization is projected to affect more than 50% of all arable land by 2050. In addition, the growing demand for food, together with the increase in the world population, [...] Read more.
Soil salinization is an important stress factor that limits plant growth and yield. Increased salinization is projected to affect more than 50% of all arable land by 2050. In addition, the growing demand for food, together with the increase in the world population, forces the need to seek salt-tolerant crops. Quinoa (Chenopodium quinoa Willd.) is an Andean crop of high importance, due to its nutritional characteristics and high tolerance to different abiotic stresses. The aim of this work is to determine the physiological, anatomical, and biochemical salt-tolerance mechanisms of a salt-tolerant (Vikinga) and a salt-sensitive (Regalona) quinoa variety. Plants were subjected to salinity stress for 15 days, starting at 100 mM NaCl until progressively reaching 400 mM NaCl. Physiological, anatomical, and biochemical parameters including growth, chlorophyll content, quantum yield of PSII (ϕPSII), gas exchange, stomatal density, size, and lipid peroxidation (via malondialdehyde, MDA) were measured. Results show that chlorophyll content, ϕPSII, and MDA were not significantly reduced under saline stress in both varieties. The most stress-affected process was the CO2 net assimilation, with an up to 60% reduction in both varieties, yet Vikinga produced higher dry weight than Regalona due to the number of leaves. The stomatal densities increased under salinity for both varieties, with Regalona the one showing higher values. The averaged stomatal size was also reduced under salinity in both varieties. The capacity of Vikinga to generate higher dry weight is a function of the capacity to generate greater amounts of leaves and roots in any condition. The stomatal control is a key mechanism in quinoa’s salinity tolerance, acquiring higher densities with smaller sizes for efficient management of water loss and carbon assimilation. These findings highlight the potential of Vikinga for cultivation in temperate salinized environments during winter, such as Deltas and lowlands where rice is grown during summer. Full article
(This article belongs to the Section Plant-Crop Biology and Biochemistry)
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<p>Scheme summarizing the experiment. Grey to black colors represent low (100 mM) to high (400 mM) NaCl concentration in the nutritive solutions.</p>
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<p>Growth measurements of Vikinga and Regalona varieties under salinity conditions (400 mM NaCl): (<b>A</b>) plant height (cm); (<b>B</b>) fresh weight of the whole plant (g) and fresh weight of the leaves (dark grey), stem (pale grey), and root (black); (<b>C</b>) shoot/root ratio; (<b>D</b>) dry weight (g) of the whole plant and dry weight of the leaves (dark grey), stem (pale grey), and root (black). Data are means of twenty-four repetitions (<span class="html-italic">n</span> = 24) ± standard error. According to Tukey’s multiple-comparisons test, significant differences with a <span class="html-italic">p</span> &lt; 0.05 have been represented with different letters.</p>
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<p>(<b>A</b>) SPAD, (<b>B</b>) quantum yield of photosystem II, (<b>C</b>) net CO<sub>2</sub> assimilation (A<sub>n</sub>), (<b>D</b>) stomatal conductance (g<sub>s</sub>), (<b>E</b>) intercellular concentration of CO<sub>2</sub> (C<sub>i</sub>), and (<b>F</b>) leaf temperature for Vikinga (closed bars) and Regalona (open bars) varieties. Data are means of twenty-four repetitions (n = 24) ±standard error. According to Tukey’s multiple-comparisons test, significant differences with a <span class="html-italic">p</span> &lt; 0.05 have been represented with different letters.</p>
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<p>(<b>A</b>) Stomatal density of the adaxial surface of the leaves and (<b>B</b>) Stomatal density of the abaxial surface of the leaves for Vikinga (black bars) and Regalona (white bars) varieties. According to Tukey’s multiple—comparisons test, significant differences with a <span class="html-italic">p</span> &lt;0.05.</p>
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<p>(<b>A</b>,<b>B</b>) Microscopic images of the adaxial and abaxial surface of the Vikinga variety in the control treatment. (<b>C</b>,<b>D</b>) Microscopic images of the adaxial and abaxial surface of the Regalona variety under control treatment. (<b>E</b>,<b>F</b>) Microscopic images of the adaxial and abaxial surface, respectively, of the Vikinga variety in the saline treatment (400 mM NaCl). (<b>G</b>,<b>H</b>) Microscopic images of the adaxial and abaxial surface, respectively, of the Regalona variety in the saline treatment (400 mM NaCl).</p>
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<p>Length and width of adaxial and abaxial stomata comparing Vikinga and Regalona under salinity (400 mM NaCl) and control conditions (0 mM NaCl). Data means of thirty-two repetitions (n = 32) ± standard error. According to Tukey’s multiple-comparisons test, significant differences with a <span class="html-italic">p</span> &lt; 0.05 have been represented with capital letters when comparing stomata lengths and lower-case letters when comparing widths.</p>
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<p>MDA values (nm g<sup>−1</sup> PF) for Vikinga (black bars) and Regalona (white bars). The MDA data are means of four repetitions (n = 4). According to Tukey’s multiple-comparisons test, significant differences with a <span class="html-italic">p</span> &lt; 0.05 have been represented with different letters.</p>
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14 pages, 2117 KiB  
Article
Strategic Switching from Conventional Urea to Nano-Urea for Sustaining the Rice–Wheat Cropping System
by Ashwani Kumar, Parvender Sheoran, Sunita Devi, Naresh Kumar, Kapil Malik, Manu Rani, Arvind Kumar, Pooja Dhansu, Shruti Kaushik, Ajay Kumar Bhardwaj, Anita Mann and Rajender Kumar Yadav
Plants 2024, 13(24), 3523; https://doi.org/10.3390/plants13243523 - 17 Dec 2024
Viewed by 204
Abstract
In the face of declining crop yields, inefficient fertilizer usage, nutrient depletion, and limited water availability, the efficiency of conventional NPK fertilizers is a critical issue in India. The hypothesis of this study posits that nano-nitrogen could enhance growth and photosynthetic efficiency in [...] Read more.
In the face of declining crop yields, inefficient fertilizer usage, nutrient depletion, and limited water availability, the efficiency of conventional NPK fertilizers is a critical issue in India. The hypothesis of this study posits that nano-nitrogen could enhance growth and photosynthetic efficiency in crop plants compared to conventional fertilizers. For this, a randomized block design (RBD) field experiment was conducted with six treatments: no nitrogen (T1), 100% N through urea (T2), and varying levels of N replacement with nano-nitrogen (33%: T3; 50%: T4; 66%: T5; and 100%: T6). Morphological and physiological traits and yield attributes were measured at physiological maturity, and yield attributes were measured at harvest. Results showed that 33% nitrogen replacement with nano-nitrogen (T3) outperformed conventional urea (T2) in physiological traits and achieved higher grain yields (3789 kg/ha for rice and 4206 kg/ha for wheat) compared to T2 (3737 kg/ha for rice and 4183 kg/ha for wheat with 100% urea). Although T4 and T5 showed statistically similar yields, they were lower than T2 and T3 for rice, while 50%, 66%, and 100% replacements reduced wheat yield by 2.49%, 8.39%, and 41.26%, respectively, compared to T2. Key enzymes of N metabolism decreased with higher nano-nitrogen substitution. Maximum nitrogen availability was observed in T2 and T3. This study concludes that nano-nitrogen is an effective strategy to enhance growth, balancing productivity and environmental sustainability. Full article
(This article belongs to the Special Issue Role of Nitrogen in Plant Growth and Development)
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<p>Effect of nitrogen substitution through nano-nitrogen on test weight (g), grain yield (kg/ha), and straw yield (kg/ha) of rice and wheat crop. (<b>A</b>)—the lowercase letter represented significant differences between treatments in straw yield of rice crop while uppercase letters represented differences in grain yield of rice crop; (<b>B</b>)—the lowercase letter represented significant differences between treatments in straw yield of wheat crop while uppercase letters represented differences in grain yield of wheat crop; (<b>C</b>)—the uppercase letter represented significant differences between treatments in Test weight of rice crop while lowercase letters represented differences in Test weight of wheat crop).</p>
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<p>The effect of nitrogen substitution through nano-nitrogen on RWC (%) and Leaf area (cm<sup>2</sup>/plant) of rice and wheat crop (means followed by at least one letter common are not statistically significant (<span class="html-italic">p</span> &lt; 0.05) using LSD test). The uppercase letter represented significant differences between treatments in rice crop while lowercase letters represented differences in wheat crop.</p>
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<p>Effect of nitrogen substitution through nano-nitrogen on nitrogen-metabolizing enzymes in rice and wheat crops (means followed by at least one letter common are not statistically significant (<span class="html-italic">p</span> &lt; 0.05) using LSD test). The uppercase letter represented significant differences between treatments in rice crop while lowercase letters represented differences in wheat crop.</p>
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<p>Effect of nitrogen substitution through nano-nitrogen on available soil nitrogen in rice and wheat crops.</p>
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17 pages, 759 KiB  
Article
Optimizing Productivity and Resource Use Efficiency Under a Finger Millet-Based Cropping System
by Sukanya T. Shivalingaiah, Sneha M. Anajaneyulu, Chaithra Chandrappa and Ragimasalawada Madhusudhana
Sustainability 2024, 16(24), 11046; https://doi.org/10.3390/su162411046 - 17 Dec 2024
Viewed by 291
Abstract
Finger millet, known for its resilience to adverse climatic conditions, is integrated with various crops to assess the synergistic benefits of intercropping. To obtain intercropping system benefits, crop association, and species combination play a crucial role. Hence, to augment the productivity, profitability, and [...] Read more.
Finger millet, known for its resilience to adverse climatic conditions, is integrated with various crops to assess the synergistic benefits of intercropping. To obtain intercropping system benefits, crop association, and species combination play a crucial role. Hence, to augment the productivity, profitability, and resource use efficiency under the millet-based system, field research was initiated for three kharif seasons (2021, 2022, and 2023) at the Project Coordinating Unit, University of Agricultural Sciences, Bangalore, Karnataka, India. The outcomes indicated that crops under sole cropping outperformed their intercropping structure in yield. Amongst the intercropping systems, finger millet and groundnut at a 4:2 exhibited a significantly higher finger millet grain equivalent yield (3065 kg/ha), land equivalent ratio (1.64), and area time equivalent ratio (1.38). Also, net returns (Rs. 73,276 ha−1) were realized to be higher in the finger millet + groundnut intercropping system at 4:2 row proportion. Finger millet as a sole crop showed a higher energy output (72,432 MJ ha−1), net energy gain (60,227 MJ ha−1), and energy efficiency (5.95) in relation to other cropping systems. Still, it was analogous to finger millet + groundnut (62,279 MJ ha−1 and 60,378 MJ ha−1, 49,623 MJ ha−1 and 47,628 MJ ha−1, 4.93 and 4.74) at 6:2 and 4:2 row extents, correspondingly). The intercropping of the finger millet with groundnut has demonstrated superior carbon sequestration competencies making them more sustainable and carbon-efficient options compared to sole crops like niger, which showed net carbon loss. The present investigation concluded the adoption of the finger millet + groundnut (4:2) intercropping system as a feasible substitute for attaining overall enhanced productivity with profitability, resource use efficiency, carbon, and energy efficiency. Full article
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<p>Operation-wise energy consumption of finger millet-based intercropping system.</p>
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<p>Source-wise estimated total carbon input in different cropping systems (kg CO<sub>2</sub>-eq. ha<sup>−1</sup>), kg CO<sub>2</sub>-eq. ha<sup>−1</sup> = kg carbon dioxide equivalent per hectare.</p>
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<p>Simple linear regression relationship between yield and nutrient uptake.</p>
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14 pages, 1681 KiB  
Article
Changes in Endogenous Carotenoids, Flavonoids, and Phenolics of Drought-Stressed Broccoli Seedlings After Ascorbic Acid Preconditioning
by Linqi Cai, Lord Abbey and Mason MacDonald
Plants 2024, 13(24), 3513; https://doi.org/10.3390/plants13243513 - 16 Dec 2024
Viewed by 314
Abstract
Drought is an abiotic disturbance that reduces photosynthesis, plant growth, and crop yield. Ascorbic acid (AsA) was utilized as a seed preconditioning agent to assist broccoli (Brassica oleracea var. italica) in resisting drought. However, the precise mechanism by which AsA improves [...] Read more.
Drought is an abiotic disturbance that reduces photosynthesis, plant growth, and crop yield. Ascorbic acid (AsA) was utilized as a seed preconditioning agent to assist broccoli (Brassica oleracea var. italica) in resisting drought. However, the precise mechanism by which AsA improves seedlings’ development remains unknown. One hypothesis is that AsA works via antioxidant mechanisms and reduces oxidative stress. This study aims to confirm the effect of varied concentrations of AsA (control, 0 ppm, 1 ppm, or 10 ppm) on seedling growth and changes in the antioxidant status of broccoli seedlings under regular watering or drought stress. AsA increased shoot dry mass, leaf area, net photosynthesis, and water use efficiency in watered and drought-stressed seedlings. AsA significantly (p < 0.001) increased carotenoid content in watered and drought-stressed seedlings by approximately 27% and 111%, respectively. Drought increased chlorophyll b, flavonoids, phenolics, ascorbate, and hydrogen peroxide production in control seedlings, but either had no effect or less effect on plants preconditioned with 10 ppm AsA. There was no improvement in reactive oxygen species scavenging in AsA-preconditioned seedlings compared to the control. The absence or reduction in biochemical indicators of stress suggests that preconditioned broccoli seedlings do not perceive stress the same as control seedlings. In conclusion, the consistent increase in carotenoid concentration suggests that carotenoids play some role in the preconditioning response, though the exact mechanism remains unknown. Full article
(This article belongs to the Special Issue Vegetable and Fruit Production, 2nd Edition)
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<p>(<b>a</b>) Shoot dry biomass of broccoli seedlings; (<b>b</b>) leaf area of broccoli seedlings. Each figure compares four seed preconditioning treatments in both watered and drought conditions. Bars represent an average of 8 replicates for each treatment combination. Bars with different letters are significantly different based on Tukey’s multiple means comparison at 5% significance.</p>
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<p>(<b>a</b>) Net photosynthesis (Pn); (<b>b</b>) evapotranspiration (E); (<b>c</b>) stomatal conductance (Gs); (<b>d</b>) water use efficiency (WUE) of broccoli seedlings. Each figure compares four seed preconditioning treatments in both watered and drought conditions. Bars represent an average of 8 replicates for each treatment combination. Bars with different letters are significantly different based on Tukey’s multiple means comparison at 5% significance.</p>
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<p>(<b>a</b>) Hydrogen peroxide production and (<b>b</b>) ROS scavenging in broccoli seedlings in 4 seed preconditioning treatments in broccoli seedlings that were watered or exposed to drought. Bars represent an average of 8 replicates for each treatment combination. Bars with different letters are significantly different based on Tukey’s multiple means comparison at 5% significance.</p>
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<p>(<b>a</b>) Principal component score plot, with points identified by treatment. Shaded areas indicate clusters of points associated with drought or watered conditions; (<b>b</b>) principal component loading plot. Pn = net photosynthesis, E = transpiration, Gs = stomatal conductance, WUE = water use efficiency, Chl a and b = chlorophyll a and b, and ROS = reactive oxygen species.</p>
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