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Search Results (2,248)

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Keywords = collective movement

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15 pages, 3160 KiB  
Article
Genomic Insights into the Population Genetics and Adaptive Evolution of Yellow Seabream (Acanthopagrus latus) with Whole-Genome Resequencing
by Yuan Li, Jingyu Yang, Yan Fang, Ran Zhang, Zizi Cai, Binbin Shan, Xing Miao, Longshan Lin, Puqing Song and Jing Zhang
Animals 2025, 15(5), 745; https://doi.org/10.3390/ani15050745 - 5 Mar 2025
Viewed by 202
Abstract
Yellow seabream (Acanthopagrus latus), a species of significant economic importance, predominantly inhabits the warm waters of the Indo-Western Pacific. While previous studies have explored the genetic diversity of A. latus using microsatellites and other nuclear markers, a comprehensive understanding of its [...] Read more.
Yellow seabream (Acanthopagrus latus), a species of significant economic importance, predominantly inhabits the warm waters of the Indo-Western Pacific. While previous studies have explored the genetic diversity of A. latus using microsatellites and other nuclear markers, a comprehensive understanding of its genetic characteristics and adaptive evolution using whole-genome resequencing (WGR) remains limited. In this study, we collected 60 individuals from six distinct geographic locations and performed WGR, achieving an average sequencing depth of 12.59×, which resulted in the identification of 19,488,059 high-quality single-nucleotide polymorphisms (SNPs). The nucleotide polymorphism (πθ) across all populations was consistent, ranging from 0.003042 to 0.003155, indicating low genetic differentiation among populations. Comparative analyses revealed that populations other than that in Xiamen (XM) have undergone adaptive evolution, potentially linked to traits such as growth and development, feeding, immunity, and movement. This study explores the population genetics and adaptive evolutionary patterns of Acanthopagrus latus at the genomic level, providing an essential foundation for the conservation and management of this economically important species in the future. Full article
(This article belongs to the Section Animal Genetics and Genomics)
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<p>Sampling sites of <span class="html-italic">A. latus</span> (the red dots indicate the sampling locations, and the arrows represent the coastal currents along the South China coast during autumn and winter. A morphological diagram of <span class="html-italic">A. latus</span> is shown in the lower right corner).</p>
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<p>The distribution of SNPs (<b>A</b>) and InDels (<b>B</b>).</p>
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<p>The phylogenetic relationship of six <span class="html-italic">A. latus</span> populations. (<b>A</b>) The NJ tree of six <span class="html-italic">A. latus</span> populations based on all SNPs. (<b>B</b>) Cross-validation (CV) error for varying values of K. (<b>C</b>) Population genetic structure of <span class="html-italic">A. latus</span>. The length of each color fragment indicates the proportion of individual genes inferred from the ancestral population (K = 2~6), and sample names are at the bottom. Each color represents a different hypothetical ancestor. (<b>D</b>) Gene flow of <span class="html-italic">A. latus</span> among the six populations. The five yellow arrows correspond to the five gene flow events identified in the analysis.</p>
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<p>Demographic history of <span class="html-italic">A. latus</span>.</p>
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<p>GO (<b>A</b>) and KEGG (<b>B</b>) enrichment analyses for selected genes in Xiamen and five other locations with <span class="html-italic">A. latus</span> populations.</p>
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<p>The identification of selection sweeps between Xiamen (XM) and five other locations for <span class="html-italic">A. latus</span>. (<b>A</b>) ROD values along chromosomes (the yellow/blue dots represents the ROD value of all SNPs, and the red dashed line represents the threshold line of the top 5% of ROD). (<b>B</b>) The NJ tree of selected genes. (<b>C</b>) <span class="html-italic">F<sub>st</sub></span>, π<sub>θ</sub>, and Tajima’s D near the <span class="html-italic">NFIC</span> gene. (<b>D</b>) <span class="html-italic">F<sub>st</sub></span>, π<sub>θ</sub>, and Tajima’s D near the <span class="html-italic">RAC2</span> gene. The yellow highlight indicates gene regions with strong selective signals. The green wavy line represents the genetic differentiation analysis between the Xiamen population and other populations.</p>
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36 pages, 653 KiB  
Systematic Review
Machine Learning-Based Computer Vision for Depth Camera-Based Physiotherapy Movement Assessment: A Systematic Review
by Yafeng Zhou, Fadilla ’Atyka Nor Rashid, Marizuana Mat Daud, Mohammad Kamrul Hasan and Wangmei Chen
Sensors 2025, 25(5), 1586; https://doi.org/10.3390/s25051586 - 5 Mar 2025
Viewed by 188
Abstract
Machine learning-based computer vision techniques using depth cameras have shown potential in physiotherapy movement assessment. However, a comprehensive understanding of their implementation, effectiveness, and limitations remains needed. Following PRISMA guidelines, we systematically reviewed studies from 2020 to 2024 across Web of Science, Scopus, [...] Read more.
Machine learning-based computer vision techniques using depth cameras have shown potential in physiotherapy movement assessment. However, a comprehensive understanding of their implementation, effectiveness, and limitations remains needed. Following PRISMA guidelines, we systematically reviewed studies from 2020 to 2024 across Web of Science, Scopus, PubMed, and Astrophysics Data System to explore recent advancements. From 371 initially identified publications, 18 met the inclusion criteria for detailed analysis. The analysis revealed three primary implementation scenarios: local (50%), clinical (33.4%), and remote (22.3%). Depth cameras, particularly the Kinect series (65.4%), dominated data collection methods. Data processing approaches primarily utilized RGB-D (55.6%) and skeletal data (27.8%), with algorithms split between traditional machine learning (44.4%) and deep learning (41.7%). Key challenges included limited real-world validation, insufficient dataset diversity, and algorithm generalization issues, while machine learning-based computer vision systems demonstrated effectiveness in movement assessment tasks, further research is needed to address validation in clinical settings and improve algorithm generalization. This review provides a foundation for enhancing computer vision-based assessment tools in physiotherapy practice. Full article
(This article belongs to the Section Sensing and Imaging)
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<p>The structure of this article.</p>
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<p>PRISMA diagram of the systematic review.</p>
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<p>Boolean Search Strategy for Computer Vision and Physiotherapy Movements.</p>
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<p>Sensor selection: depth-camera vs. other sensors.</p>
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<p>Survey of sensor in physiotherapy movement assessment.</p>
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<p>Pie chart of percentage distribution of data types.</p>
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<p>Applications and statistics of various algorithms in physiotherapy movement assessment.</p>
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<p>Diagram of bars for the algorithm in current literature.</p>
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<p>Pie chart depicting research application scenarios for physiotherapy movement assessment.</p>
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<p>Diagram of bars for the studied body parts.</p>
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11 pages, 530 KiB  
Article
Correlation of the Asymmetry Index from the Single-Leg Countermovement Jump with the Asymmetry Index from Isokinetic Strength in Elite Youth Football Players
by Yiannis Michailidis, Andreas Stafylidis, Athanasios Mandroukas, Angelos E. Kyranoudis, Georgios Antoniou, Rossetos Kollias, Vasileios Kanaras, Charalampos Bamplekis, Lazaros Vardakis, Eleni Semaltianou and Thomas I. Metaxas
Appl. Sci. 2025, 15(5), 2779; https://doi.org/10.3390/app15052779 - 5 Mar 2025
Viewed by 509
Abstract
Football is a sport in which athletes perform many movements using one of their legs, which is characterized as dominant. This differentiation in usage often creates asymmetries between the limbs, which has been noted to be related to the occurrence of injuries as [...] Read more.
Football is a sport in which athletes perform many movements using one of their legs, which is characterized as dominant. This differentiation in usage often creates asymmetries between the limbs, which has been noted to be related to the occurrence of injuries as well as negative effects on performance. To assess these asymmetries, vertical jump tests, as well as tests using an isokinetic dynamometer, were employed. The aim of this study was to examine the relationship between the asymmetries observed in the application of the single-leg countermovement jump (SLCMJ) and those observed through isokinetic strength assessment at 60°/s, 180°/s, and 300°/s in high-level youth football players. The study involved 63 elite youth football players. For statistical analysis, correlation analysis and linear regression analysis were used. The results showed a significant positive correlation between the CMJ Bilateral Asymmetry Index and the BAI Anterior Quadriceps (60°/s) (r = 0.262, p = 0.038, 95% CI [0.016, 0.479]), indicating a small-to-moderate effect size (Fisher’s z = 0.269, SE = 0.129). Additionally, a significant negative correlation was identified between the CMJ Bilateral Asymmetry Index and the BAI Posterior Hamstrings (60°/s) (r = −0.319, p = 0.011, 95% CI [−0.525, −0.077]), demonstrating a moderate inverse relationship (Fisher’s z = −0.331, SE = 0.129). The overall regression model was significant, F(6,56) = 2.42, p = 0.038, R2 = 0.206, indicating that the predictors collectively explained approximately 20.6% of the variance in the CMJ Bilateral Asymmetry Index. From the findings of this study, we conclude that the SLCMJ asymmetry index cannot replace the asymmetry index from isokinetic strength tests. Full article
(This article belongs to the Special Issue Advances in Sport Physiology, Nutrition, and Metabolism)
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<p>Pearson’s correlation matrix of CMJ Bilateral Asymmetry Index (BAI) and isokinetic variables across different angular velocities. The heatmap displays Pearson’s correlation coefficients (<span class="html-italic">r</span>) between the CMJ Bilateral Asymmetry Index and biomechanical variables, including BAI Anterior Quadriceps (60°/s, 180°/s, 300°/s) and BAI Posterior Hamstrings (60°/s, 180°/s, 300°/s). Significant correlations are indicated with * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001. Warmer colors indicate positive correlations, while cooler colors represent negative correlations.</p>
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14 pages, 6650 KiB  
Article
Development of a Biomechanical Diagnosis and Analysis System Using a Textile Elbow Angle Sensor: Integrating Inverse Dynamics and Multi-Layer Perceptron Techniques
by Sang-Un Kim and Joo-Yong Kim
Processes 2025, 13(3), 748; https://doi.org/10.3390/pr13030748 - 4 Mar 2025
Viewed by 283
Abstract
The recent development of algorithms through artificial intelligence and the ability to measure the human body through soft textile sensors has enabled the provision of meaningful information to the wearer. In this study, a sensor sleeve using a textile elbow angle sensor that [...] Read more.
The recent development of algorithms through artificial intelligence and the ability to measure the human body through soft textile sensors has enabled the provision of meaningful information to the wearer. In this study, a sensor sleeve using a textile elbow angle sensor that can measure the bending and relaxation of the elbow was manufactured and measured. In addition, biomechanical data from Biomechanical of Bodies (BoB)-4, a software capable of inverse dynamics that can optimally calculate the load on human joints and segments during exercise, was collected. A continuous system of resistance angle and angle biomechanical data was designed with an artificial intelligence multilayer perceptron (MLP) algorithm, and the accuracy and output results were checked. Consequently, the accuracy of MLP1 and MLP2 is exceedingly high, at approximately 0.80 and 1.00, respectively. The biomechanical data of the system is comparable to that of BoB, rendering it suitable for providing reliable information to the wearer. Based on this study, it is possible to develop algorithms and systems that can perform biomechanical analysis for various exercise movements in the future. Full article
(This article belongs to the Special Issue Research on Intelligent Fault Diagnosis Based on Neural Network)
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<p>Mechanism of arm bending.</p>
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<p>Dip-coating process of textile elbow angle sensor and making sensor arm sleeve.</p>
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<p>Measuring sensor properties of the textile elbow angle sensor using UTM.</p>
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<p>The block diagram of the process of the biomechanical diagnosis and analysis system.</p>
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<p>The mechanical analysis of bicep brachialis using BOB.</p>
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<p>The structure of MLP1 and MLP2.</p>
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<p>The results of the resistance change ratio depend on the strain of the textile elbow angle sensor (<b>a</b>) and the angle of the sensor sleeve (<b>b</b>).</p>
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<p>Modeling result of the MLP1.</p>
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<p>The comparison results of the IMU and MLP1 data of resistance angle (<b>a</b>) and bicep curl 10 times (<b>b</b>).</p>
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<p>Modeling result of the MLP2.</p>
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<p>The comparison of biomechanical data between the BoB and MLP2.</p>
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23 pages, 7150 KiB  
Article
An IoT-Enabled Wearable Device for Fetal Movement Detection Using Accelerometer and Gyroscope Sensors
by Atcharawan Rattanasak, Talit Jumphoo, Wongsathon Pathonsuwan, Kasidit Kokkhunthod, Khwanjit Orkweha, Khomdet Phapatanaburi, Pattama Tongdee, Porntip Nimkuntod, Monthippa Uthansakul and Peerapong Uthansakul
Sensors 2025, 25(5), 1552; https://doi.org/10.3390/s25051552 - 2 Mar 2025
Viewed by 367
Abstract
Counting fetal movements is essential for assessing fetal health, but manually recording these movements can be challenging and inconvenient for pregnant women. This study presents a wearable device designed to detect fetal movements across various settings, both within and outside medical facilities. The [...] Read more.
Counting fetal movements is essential for assessing fetal health, but manually recording these movements can be challenging and inconvenient for pregnant women. This study presents a wearable device designed to detect fetal movements across various settings, both within and outside medical facilities. The device integrates accelerometer and gyroscope sensors with Internet of Things (IoT) technology to accurately differentiate between fetal and non-fetal movements. Data were collected from 35 pregnant women at Suranaree University of Technology (SUT) Hospital. This study evaluated ten signal extraction methods, six machine learning algorithms, and four feature selection techniques to enhance classification performance. The device utilized Particle Swarm Optimization (PSO) for feature selection and Extreme Gradient Boosting (XGB) with PSO hyper-tuning. It achieved a sensitivity of 90.00%, precision of 87.46%, and an F1-score of 88.56%, reflecting commendable results. The IoT-enabled technology facilitated continuous monitoring with an average latency of 423.6 ms. It ensured complete data integrity and successful transmission, with the capability to operate continuously for up to 48 h on a single charge. The findings substantiate the efficacy of the proposed approach in detecting fetal movements, thereby demonstrating a practical and valuable technology for fetal movement detection applications. Full article
(This article belongs to the Section Internet of Things)
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<p>Overview of the proposed fetal movement monitoring system framework.</p>
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<p>Overview of the wearable fetal movement monitoring system components.</p>
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<p>Fetal movement monitoring system with mobile interface (<b>a</b>). The system architecture for fetal movement monitoring. (<b>b</b>) The user interface of the mobile application designed for pregnant women.</p>
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<p>Real-world using wearable fetal movement detection device with mobile application. (<b>a</b>) A wearable device on a pregnant woman, connected to a smartphone for fetal monitoring. (<b>b</b>) A smartphone displaying real-time fetal movement data.</p>
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<p>(<b>a</b>–<b>c</b>) represent the accelerometer signals along the <span class="html-italic">x</span>, <span class="html-italic">y</span>, and <span class="html-italic">z</span> axes, respectively. (<b>d</b>–<b>f</b>) represent the gyroscope signals along the <span class="html-italic">x</span>, <span class="html-italic">y</span>, and <span class="html-italic">z</span> axes, respectively. (<b>g</b>) The signal from the button device, indicating the moments when the mother perceives fetal movements. It has been observed that the perception of fetal movements by the mother lags behind the actual detected fetal movement signals.</p>
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<p>ROC curve comparison for various classification models.</p>
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16 pages, 565 KiB  
Article
On the Determination of Centers of Mass via Fractal Calculus and Its Applications in Board Games
by Josué N. Gutiérrez-Corona, Israel Garduño-Bonilla, Luis A. Quezada-Téllez, Guillermo Fernández-Anaya and Jorge E. Macías-Díaz
Symmetry 2025, 17(3), 381; https://doi.org/10.3390/sym17030381 - 2 Mar 2025
Viewed by 204
Abstract
This study introduces a novel approach to chess analysis based on center-of-mass dynamics and discrete fractal derivatives, offering an alternative framework for evaluating gameplay strategies. Unlike conventional methods that rely on exhaustive search and statistical simulations, our model provides a macroscopic perspective by [...] Read more.
This study introduces a novel approach to chess analysis based on center-of-mass dynamics and discrete fractal derivatives, offering an alternative framework for evaluating gameplay strategies. Unlike conventional methods that rely on exhaustive search and statistical simulations, our model provides a macroscopic perspective by analyzing the collective motion of pieces over time. By representing chess positions as a dynamic system in R2, we identify key movement patterns—such as oblique, parallel, and orthogonal trends—revealing strategic tendencies throughout the game. Additionally, fractal derivatives enable the detection of subtle momentum shifts and long-term imbalances, enhancing the understanding of decision-making processes. This approach is computationally efficient and adaptable, extending beyond chess to applications in sports analytics and real-time strategy games. These findings highlight the potential of interdisciplinary techniques in capturing complex strategic behavior within dynamic environments. Full article
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<p>Piece movement trends in the Carlsen vs. Nepomniachtchi match, World Chess Championship, 3 December 2021. It can be observed that the lines tend to follow an oblique pattern with a low slope, indicating that both players had attacking intentions on the left flank.</p>
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<p>Plot of velocity using fractal derivatives with <math display="inline"><semantics> <mrow> <mi>α</mi> <mo>=</mo> <mi>β</mi> <mo>=</mo> <mn>0.5</mn> </mrow> </semantics></math>. The opposite directions of velocity trends reflect the attacking strategies of both players, with the black player advancing toward the white side and the white player attacking the black side.</p>
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<p>Velocity plot using fractal derivatives with <math display="inline"><semantics> <mrow> <mi>α</mi> <mo>=</mo> <mn>0.4</mn> </mrow> </semantics></math> and <math display="inline"><semantics> <mrow> <mi>β</mi> <mo>=</mo> <mn>0.5</mn> </mrow> </semantics></math>. As opposing directional trends remain evident, the angular relationships between vectors become more pronounced, exhibiting a greater degree of perpendicularity.</p>
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<p>Piece movement trends in the Kasparov vs. Karpov match, World Chess Championship, 15 October 1985.</p>
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<p>Velocity analysis using fractal derivatives with <math display="inline"><semantics> <mrow> <mi>α</mi> <mo>=</mo> <mi>β</mi> <mo>=</mo> <mn>0.5</mn> </mrow> </semantics></math>. The parallel trends emphasize the strategic queenside focus by both players.</p>
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<p>Piece movement trends in the Spassky vs. Fischer match, World Chess Championship, 16 July 1972.</p>
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<p>Velocity analysis using fractal derivatives with <math display="inline"><semantics> <mrow> <mi>α</mi> <mo>=</mo> <mi>β</mi> <mo>=</mo> <mn>0.5</mn> </mrow> </semantics></math>. The trends emphasize the focus on the a1-h8 diagonal and opposing directional tendencies. The red and blue lines represent the same as in <a href="#symmetry-17-00381-f005" class="html-fig">Figure 5</a>.</p>
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17 pages, 5038 KiB  
Article
Motion Gait Recognition of Lower Limb Exoskeleton Based on Particle Swarm Optimization-Based Extreme Learning Machine Algorithm
by Ting Liu, Kai Liu, Wuyi Luo, Jiange Kou, Haoran Zhan, Guangkai Yu, Qing Guo and Yan Shi
Actuators 2025, 14(3), 120; https://doi.org/10.3390/act14030120 - 2 Mar 2025
Viewed by 248
Abstract
A human gait recognition method based on the PSO-ELM algorithm is proposed in order to achieve coordinated movement between humans and lower limb exoskeletons. Ground reaction force (GRF) from the foot, and motion capture data (MCD) from two joints were collected through the [...] Read more.
A human gait recognition method based on the PSO-ELM algorithm is proposed in order to achieve coordinated movement between humans and lower limb exoskeletons. Ground reaction force (GRF) from the foot, and motion capture data (MCD) from two joints were collected through the exoskeleton device. The sample data were obtained through multiple experiments in different action scenarios, including standing still, walking on the flat, climbing up and down stairs, traveling up and down slopes, in addition to squatting down and standing up. The algorithm utilizes short-term posture data to recognize different posture movement patterns, with two advantages: (1) A user-friendly wearable device was constructed based on multi-source sensors distributed throughout the body, addressing multiple subjects with varying weights and heights, while being cost-effective and reliably and easily collecting data. (2) The PSO-ELM algorithm identifies key features of gait data, achieving a higher recognition accuracy than other advanced recognition methods, especially during arbitrary gait transition duration. Full article
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<p>Exoskeleton device.</p>
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<p>Config interface.</p>
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<p>IK interface.</p>
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<p>Motion capture.</p>
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<p>The gait acquisitions experiments under different motion patterns.</p>
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<p>The position of the left and right legs, knees, hips, and the soles of the left and right feet during walking.</p>
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<p>The smoothing effect of the left heel GRF signal: (<b>a</b>) original GRF signal; (<b>b</b>) processed GRF signal.</p>
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<p>The MCD before and after processing: (<b>a</b>) the original MCD signal; (<b>b</b>) the processed MCD signal.</p>
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<p>The structure of ELM.</p>
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<p>The flow of the PSO-ELM model.</p>
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<p>The training process and results of the PSO-ELM model of Subject 1: (<b>a</b>) the training process; (<b>b</b>) the confusion matrix.</p>
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<p>The training process and results of the FNN and the LSTM models of Subject 1: (<b>a</b>) the training process of the FNN; (<b>b</b>) the confusion matrix of the FNN; (<b>c</b>) the training process of the LSTM model; (<b>d</b>) the confusion matrix of the LSTM model.</p>
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<p>The recognition precision of the PSO-ELM model for each gait phase of Subject 1.</p>
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<p>The recognition precision for each gait phase of Subject 1: (<b>a</b>) the FNN; (<b>b</b>) the LSTM model.</p>
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<p>The recognition precision of the PSO-ELM model for each gait phase of Subject 1.</p>
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<p>The recognition precision for each gait phase of Subject 1: (<b>a</b>) the FNN; (<b>b</b>) the LSTM model.</p>
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28 pages, 72675 KiB  
Article
Geochemical and Isotopic Features of Geothermal Fluids Around the Sea of Marmara, NW Turkey
by Francesco Italiano, Heiko Woith, Luca Pizzino, Alessandra Sciarra and Cemil Seyis
Geosciences 2025, 15(3), 83; https://doi.org/10.3390/geosciences15030083 - 1 Mar 2025
Viewed by 239
Abstract
Investigations carried out on 72 fluid samples from 59 sites spread over the area surrounding the Sea of Marmara show that their geochemical and isotopic features are related to different segment settings of the North Anatolian Fault Zone (NAFZ). We collected fluids from [...] Read more.
Investigations carried out on 72 fluid samples from 59 sites spread over the area surrounding the Sea of Marmara show that their geochemical and isotopic features are related to different segment settings of the North Anatolian Fault Zone (NAFZ). We collected fluids from thermal and mineral waters including bubbling and dissolved gases. The outlet temperatures of the collected waters ranged from 14 to 97 °C with no temperature-related geochemical features. The free and dissolved gases are a mixture of shallow and mantle-derived components. The large variety of geochemical features comes from intense gas–water (GWI) and water–rock (WRI) interactions besides other processes occurring at relatively shallow depths. CO2 contents ranging from 0 to 98.1% and helium isotopic ratios from 0.11 to 4.43 Ra indicate contributions, variable from site to site, of mantle-derived volatiles in full agreement with former studies on the NAFZ. We propose that the widespread presence of mantle-derived volatiles cannot be related only to the lithospheric character of the NAFZ branches and magma intrusions have to be considered. Changes in the vertical permeability induced by fault movements and stress accumulation during seismogenesis, however, modify the shallow/deep ratio of the released fluids accordingly, laying the foundations for future monitoring activities. Full article
(This article belongs to the Section Geochemistry)
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<p>Map of historic earthquakes in the wider Marmara region compiled from various sources [<a href="#B17-geosciences-15-00083" class="html-bibr">17</a>,<a href="#B18-geosciences-15-00083" class="html-bibr">18</a>,<a href="#B19-geosciences-15-00083" class="html-bibr">19</a>,<a href="#B20-geosciences-15-00083" class="html-bibr">20</a>]. Labels indicate the year of the event for magnitudes M ≥ 7. White lines depict active faults according to the General Directorate of Mineral Research and Exploration (MTA) [<a href="#B21-geosciences-15-00083" class="html-bibr">21</a>]; off-shore faults are taken from Armijo et al. (2002) [<a href="#B14-geosciences-15-00083" class="html-bibr">14</a>]. Orange and red lines indicate the ruptures related to the Ganos earthquake of 1912 and the Izmit/Düzce events of 1999, respectively.</p>
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<p>Map of fluid sampling sites around the Sea of Marmara. Symbols indicate color-coded water temperatures. Small white circles depict sites with bubbling gases. Values are sample numbers used in this study (see <a href="#geosciences-15-00083-t001" class="html-table">Table 1</a>). Names of geographic areas investigated are given.</p>
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<p>Piper diagram of the water samples as a function of the geographical areas. Sample labels as the ID numbers in <a href="#geosciences-15-00083-t002" class="html-table">Table 2</a>.</p>
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<p>Ca vs Mg (<b>a</b>) and HCO<sub>3</sub> (<b>b</b>). The occurrence of GWI processes allows CO<sub>2</sub> dissolution that is responsible for the observed geochemical features related to WRI resulting in dolomite and calcite dissolution to various extents. Sample labels are the same as the ID numbers in <a href="#geosciences-15-00083-t002" class="html-table">Table 2</a>.</p>
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<p>Na vs HCO<sub>3</sub> (<b>a</b>) and Na vs. Cl (<b>b</b>). The occurrence of WRI and GWI processes is responsible for the observed geochemical features. Blue star symbol = sea water. Sample labels are the same as the ID numbers in <a href="#geosciences-15-00083-t002" class="html-table">Table 2</a>. Symbol colors are as shown in <a href="#geosciences-15-00083-f003" class="html-fig">Figure 3</a>.</p>
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<p>Ca-SO<sub>4</sub> plot showing that gypsum dissolution is not the main process responsible for the SO<sub>4</sub> ions, with the water chemistry being a consequence of WRI and GWI processes. Sample labels are the same as the ID numbers in <a href="#geosciences-15-00083-t002" class="html-table">Table 2</a>. SW = sea water.</p>
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<p>δ<sup>18</sup>O–δD plot for the collected waters. Samples fall between the two reference lines representing the EMMWL (Eastern Mediterranean Meteoric Water Line; Hatvani et al., 2023 [<a href="#B62-geosciences-15-00083" class="html-bibr">62</a>]) and the GMWL (Global Meteoric Water Line; Rozanski et al., 1993 [<a href="#B63-geosciences-15-00083" class="html-bibr">63</a>]). BMWL refers to the Bursa local meteoric water line proposed by Imbach et al. (1997) [<a href="#B38-geosciences-15-00083" class="html-bibr">38</a>]. Sample labels are the same as the ID numbers in <a href="#geosciences-15-00083-t002" class="html-table">Table 2</a>.</p>
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<p>CO<sub>2</sub>-N<sub>2</sub> relationships for bubbling (filled circles) and dissolved (diamond) gases indicating the presence of two end members in the gas phase, namely the shallow atmospheric-derived N<sub>2</sub> component and the deep-originated CO<sub>2</sub>, vented over the Marmara area that mix at variable extents. Numbers indicate the sample IDs as in <a href="#geosciences-15-00083-t001" class="html-table">Table 1</a>.</p>
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<p>CO<sub>2</sub>-CH<sub>4</sub>-N<sub>2</sub> triangular diagram of the bubbling (filled circles) and dissolved (diamonds) gases showing the relative contents of the three end members N<sub>2</sub>, CO<sub>2</sub> and CH<sub>4</sub>. We plotted the N<sub>2</sub> excess with respect to the atmospheric nitrogen. The arrows highlight the GWI processes (CO<sub>2</sub> loss and increased N<sub>2</sub> and CH<sub>4</sub> contents) as well as mixings due to CO<sub>2</sub> addition from various sources that significantly changed the composition of the pristine gas phase. The numbers beside the symbols indicate the site as listed in <a href="#geosciences-15-00083-t001" class="html-table">Table 1</a>.</p>
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<p>CO<sub>2</sub> content vs δ<sup>13</sup>C<sub>CO2</sub> for the bubbling gases (<b>a</b>) and for δ<sup>13</sup>C<sub>TDIC</sub> of the dissolved gases (<b>b</b>). The plots depict a clear direct correlation between isotopic ratios and CO<sub>2</sub> and HCO<sub>3</sub> contents. The contemporary trends denote the fractionation with quantitative loss of gaseous CO<sub>2</sub> and its heavy isotope as well as the occurrence of further fractionation processes. The occurrence of similar trends followed by samples from different sites around the Marmara area suggests that the vented CO<sub>2</sub> is not solely controlled by shallow interactions with groundwaters, and that the coexistence of multiple sources has to be considered.</p>
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<p>Helium isotopic ratios (uncorrected R/Ra values) and <sup>4</sup>He/<sup>20</sup>Ne relationships for both dissolved and bubbling gases. The theoretical lines represent binary mixing trends of atmospheric helium with mantle-originated and crustal helium. The assumed end members for He-isotopic ratios and <sup>4</sup>He/<sup>20</sup>Ne ratios are ASW (1 Ra, He/Ne = 0.267: Holocer et al., 2002) [<a href="#B49-geosciences-15-00083" class="html-bibr">49</a>]; 8Ra for a MORB-type mantle; and 3.5 Ra for contaminated mantle; crust 0.05Ra and <sup>4</sup>He/<sup>20</sup>Ne ratio = 10,000. Filled circles = bubbling gases; filled diamonds = dissolved gases. Sample IDs are as reported in <a href="#geosciences-15-00083-t003" class="html-table">Table 3</a>. All error bars are within the symbol size.</p>
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<p>CO<sub>2</sub>/<sup>3</sup>He–<sup>4</sup>He. The plot shows how the vented gases are a mixture of two main components: magmatic-type and crustal-originated. Circles = bubbling gases; diamonds = dissolved gases. The arrows display the main trends affecting the composition of the gas phase.</p>
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<p>Map showing locations mentioned in the text. Numbers refer to sampling sites of this study (see <a href="#geosciences-15-00083-t001" class="html-table">Table 1</a>).</p>
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<p>Chemical composition of thermal and mineral waters around the Sea of Marmara. The diameter of the pies scales with the specific electrical conductivity of the waters. Small circles in the centre of the pies indicate the water temperature: blue—cold (&lt;20 °C); orange—hot (&gt;40 °C).</p>
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<p>Gas composition of thermal and mineral waters around the Sea of Marmara. Small white circles in the centre of the pies indicate bubbling gases.</p>
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<p>Helium isotope ratios given in R/Ra at mineral and thermal waters around the Sea of Marmara. Light purple areas depict Tertiary volcanic rocks, hatched areas mark intrusive igneous rocks of Paleozoic to Cenozoic age. Light and dark gray areas indicate Mesozoic and Paleozoic rocks, respectively. White areas are Paleogene to Quaternary sediments. Simplified geology modified from Pawlewicz et al. (1997) [<a href="#B83-geosciences-15-00083" class="html-bibr">83</a>].</p>
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25 pages, 538 KiB  
Article
Virtual Choirs in Care Homes: The Development and Early Assessment of a New Virtual Reality Choir Intervention
by Helena Daffern, Helen Weatherly, Pedro Saramago, Kim Steele, Dana Greaves, Maeve Kavanagh, Lucy Cooney, Jake Spreadborough, Stephen Honnan, Daniel Johnston and Ross Toomer
Virtual Worlds 2025, 4(1), 8; https://doi.org/10.3390/virtualworlds4010008 - 28 Feb 2025
Viewed by 218
Abstract
Engaging with music has been shown to have a positive impact on the quality of life of residents in care homes, who are known to be affected by anxiety, depression and loneliness. Based on the known benefits of in-person singing activities, a new [...] Read more.
Engaging with music has been shown to have a positive impact on the quality of life of residents in care homes, who are known to be affected by anxiety, depression and loneliness. Based on the known benefits of in-person singing activities, a new Virtual Reality (VR) choir application was developed to facilitate group singing, aiming to improve residents’ wellbeing and sense of community. Co-designed with Alzheimer Scotland, the intervention was tested in two care homes for functionality and to develop an approach towards assessing feasibility. Residents participated in scheduled sessions over a five-week period, in addition to staff engaging in independent ad hoc use of the experience with residents. Data on reactions to the intervention, the quality of life of participants and preferences about the outcome instruments were collected. The VR intervention proved technically successful, user-friendly, and allowed multiple users to sing together. Participants and staff showed strong enthusiasm for the intervention, with residents actively engaging in singing and movement, although some residents found the headsets uncomfortable. This suggests that VR choirs could be a valuable, scalable activity in care homes, especially when in-person facilitators are unavailable. Preliminary observations indicated that the intervention was not detrimental to participants’ health; however, the sample size was very small and a larger feasibility study is required to examine the intervention’s effectiveness, scalability, and cost-effectiveness. This research highlights the challenges associated with measuring the feasibility of VR interventions in residential care settings, and the value of capturing qualitative data in an ecological setting that represents the intended use of the intervention. Full article
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<p>System design.</p>
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<p>Mean scores from five participants who responded to the SAM.</p>
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15 pages, 3162 KiB  
Article
Behavioural Engagement of Holstein Friesian Dairy Cattle with Different Mounting Techniques for Salt Licks as Environmental Enrichment
by Danielle Lauren McLaughlin and Nicola Blackie
Animals 2025, 15(5), 701; https://doi.org/10.3390/ani15050701 - 27 Feb 2025
Viewed by 200
Abstract
With increasing numbers of dairy farms adopting zero-grazing systems, there is a growing need for indoor environmental enrichment methods. Enrichment is necessary to meet industry requirements and fulfil behavioural motivations, such as oral manipulation. This study evaluated the magnitude with which Holstein Freisen [...] Read more.
With increasing numbers of dairy farms adopting zero-grazing systems, there is a growing need for indoor environmental enrichment methods. Enrichment is necessary to meet industry requirements and fulfil behavioural motivations, such as oral manipulation. This study evaluated the magnitude with which Holstein Freisen cows would interact with salt lick enrichment blocks based on the mounting design. Holstein Freisen dairy cows (n = 55) were recruited from a UK dairy farm and observed over a 4-week period (n = 20 days). Three different mounting designs were utilized, low non-moveable (LNM), low moveable (LM), and high moveable (HM), and the LNM setup was repeated on week 4. These mounting designs were each observed over a five-day period and then removed for two days in-between. Data were collected by in-person observation and included cow IDs, instances of interaction, and kilograms of salt lick used per setup. The data were analysed through IBM SPSS Statistics via a One-Way Repeated Measures ANOVA and Microsoft Excel to determine significant findings and habituation. The number of new interactions significantly decreased in the HM setup compared to the LM and LNM. The supporting data of kilograms of salt lick used and total percentage of the herd utilizing the blocks, also favoured the LM setups over LNM. The LNM setup was repeated on the final week to assess the level with which cows had habituated to the environmental enrichment. Despite a significant difference between week 1 and week 4, the trends of cow interactions showed individual variability in habituation and overall negligible herd-level habituation. These findings suggest that the use of mineral licks within a dairy herd serves as effective environmental enrichment, even over extended time periods, and when implemented they are best used at low heights with the ability to have free movement. When implemented on a farm, the LM mounting design should increase the herd-level uptake of enrichment leading to a reduction in stereotypies and fulfilment of oral motivation, which is beneficial for overall cow health and welfare. Full article
(This article belongs to the Section Cattle)
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<p>High-Yielding Barn with Block Setup. The above figure is a drawing of the high-yielding pen from an aerial view. Blue blocks represent water troughs. Black circles represent cement posts positioned on the back wall of the barn. The red blocks indicate the location of the salt licks. The blocks were labelled 1–4 corresponding with the blocks, respectively, from left to right. This setup was relevant to weeks 1, 2, and 4.</p>
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<p>This figure shows the exact setup of the salt licks from day 0–4 and day 20–23 of the study. The blocks were labelled 1–4 as seen from left to right, respectively.</p>
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<p>This figure shows the exact setup of salt licks from day 7–11 of the study. The blocks were labelled 1–4 as seen from left to right, respectively.</p>
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<p>This figure shows the exact setup of salt licks from day 14–17 of the study. The blocks were labelled 1–4 as seen from left to right, respectively.</p>
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<p>Timeline of the Study.</p>
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<p>Box and Whisker Plot. <a href="#animals-15-00701-f006" class="html-fig">Figure 6</a> illustrates the pairwise comparison between the mean number of cow interactions and the block setups each week from the ANOVA analysis. * = <span class="html-italic">p</span> &lt; 0.05.</p>
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<p>The average daily cow use of salt licks. <a href="#animals-15-00701-f007" class="html-fig">Figure 7</a> shows the average percent of the total herd that interacted with the salt blocks daily depending on the setup.</p>
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<p>Amount (in kg) of salt lick used weekly. <a href="#animals-15-00701-f008" class="html-fig">Figure 8</a> shows how much salt was ingested each week of the study and is broken up further into the individual salt blocks spread throughout the yard (blocks = 4). The block numbers correlate to the numbers assigned in the methods section.</p>
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<p>Individual Cattle Interaction Trends Between Weeks. <a href="#animals-15-00701-f009" class="html-fig">Figure 9</a> shows how many cattle either increased, decreased, or stayed the same in the number of individual interactions compared week to week.</p>
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17 pages, 2630 KiB  
Article
Multimodal Deep Learning Model for Cylindrical Grasp Prediction Using Surface Electromyography and Contextual Data During Reaching
by Raquel Lázaro, Margarita Vergara, Antonio Morales and Ramón A. Mollineda
Biomimetics 2025, 10(3), 145; https://doi.org/10.3390/biomimetics10030145 - 27 Feb 2025
Viewed by 173
Abstract
Grasping objects, from simple tasks to complex fine motor skills, is a key component of our daily activities. Our approach to facilitate the development of advanced prosthetics, robotic hands and human–machine interaction systems consists of collecting and combining surface electromyography (EMG) signals and [...] Read more.
Grasping objects, from simple tasks to complex fine motor skills, is a key component of our daily activities. Our approach to facilitate the development of advanced prosthetics, robotic hands and human–machine interaction systems consists of collecting and combining surface electromyography (EMG) signals and contextual data of individuals performing manipulation tasks. In this context, the identification of patterns and prediction of hand grasp types is crucial, with cylindrical grasp being one of the most common and functional. Traditional approaches to grasp prediction often rely on unimodal data sources, limiting their ability to capture the complexity of real-world scenarios. In this work, grasp prediction models that integrate both EMG signals and contextual (task- and product-related) information have been explored to improve the prediction of cylindrical grasps during reaching movements. Three model architectures are presented: an EMG processing model based on convolutions that analyzes forearm surface EMG data, a fully connected model for processing contextual information, and a hybrid architecture combining both inputs resulting in a multimodal model. The results show that context has great predictive power. Variables such as object size and weight (product-related) were found to have a greater impact on model performance than task height (task-related). Combining EMG and product context yielded better results than using each data mode separately, confirming the importance of product context in improving EMG-based models of grasping. Full article
(This article belongs to the Special Issue Intelligent Human–Robot Interaction: 3rd Edition)
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<p>Seven zones for surface EMG placement from [<a href="#B24-biomimetics-10-00145" class="html-bibr">24</a>].</p>
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<p>CDF with the proposed cut-off threshold (700 samples), along with the percentage of rejected samples (7.77%).</p>
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<p>Data distributions by class and contextual data: (<b>a</b>) Weight. (<b>b</b>) Task height.</p>
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<p>Comparison of SPAN distributions by class: (<b>a</b>) Span 1, main span of the product. (<b>b</b>) Span 2, secondary span of the product.</p>
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<p>(<b>a</b>) CNN for EMG signals; (<b>b</b>) FC neural network for contextual data.</p>
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<p>Hybrid model architecture (M_HYBRID).</p>
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<p>Training results of the models: (<b>a</b>) EMG, (<b>b</b>) contextual, (<b>c</b>) hybrid.</p>
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<p>Confusion matrices of the models: (<b>a</b>) EMG, (<b>b</b>) contextual, (<b>c</b>) hybrid.</p>
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<p>Comparison of accuracy and loss for hybrid models. The dashed lines indicate the best values achieved.</p>
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11 pages, 2461 KiB  
Article
Development and Application of a Multiplex PCR Assay for Simultaneous Detection of Tomato Yellow Leaf Curl Virus and Tomato Leaf Curl New Delhi Virus
by Hongxia Hu, Jie Zhang, Xiaoyin Wu, Li Li and Yajuan Qian
Viruses 2025, 17(3), 322; https://doi.org/10.3390/v17030322 - 27 Feb 2025
Viewed by 264
Abstract
Tomato leaf curl New Delhi virus (ToLCNDV) and tomato yellow leaf curl virus (TYLCV) are two important viral pathogens that severely affect Solanaceae and Cucurbitaceae plants. In order to reduce the further spread of these viruses, it is crucial to establish an efficient [...] Read more.
Tomato leaf curl New Delhi virus (ToLCNDV) and tomato yellow leaf curl virus (TYLCV) are two important viral pathogens that severely affect Solanaceae and Cucurbitaceae plants. In order to reduce the further spread of these viruses, it is crucial to establish an efficient and reliable method to accurately detect the viruses. In this study, a multiplex PCR assay for the simultaneous detection of TYLCV and ToLCNDV was established. Three primer pairs designed from conserved regions within the coat protein or movement protein-encoding regions of the respective viruses were employed in the assay. The optimization of parameters such as primer concentration was set at 0.15 μM/0.15 μM, 0.25 μM/0.25 μM, and 0.50 μM/0.50 μM for ToLCNDV-DNA-A-F/R, TYLCV-F/R, and ToLCNDV-DNA-B-F/R primer pairs. At optimal primer concentrations, the multiplex PCR method demonstrates effective performance with an annealing temperature ranging from 51 °C to 66 °C. The specificity of the assay evaluated by testing against other begomoviruses showed no evidence of cross-amplification. Further sensitivity analysis performed using a serially diluted plasmid containing viral targets as templates demonstrated high sensitivity with a detection limit of 103 copies/μL. Field surveys utilizing the multiplex PCR assay successfully identified the infection of TYLCV and ToLCNDV in field-collected samples. Full article
(This article belongs to the Section Viruses of Plants, Fungi and Protozoa)
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<p>Establishment of multiplex PCR assay. Lane 1 represented the amplification products with three primer pairs (ToLCNDV-DNA-A-F/R, ToLCNDV-DNA-B-F/R, and TYLCV-F/R) with a DNA mixture containing both ToLCNDV and TYLCV as the template. Lane 2 represented the amplification products using ToLCNDV-DNA-A-F/R and ToLCNDV-DNA-B-F/R primer pairs with DNA containing only ToLCNDV as the template. Lane 3 represented the amplification products using TYLCV-F/R primer pairs using DNA containing only TYLCV as a template. Lane M corresponded to the GeneRuler 1 kb Plus DNA Ladder, and Lane CK<sup>−</sup> represented the negative control.</p>
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<p>Optimization of multiplex PCR assay. (<b>a</b>) Optimization of primer concentrations. Lanes 1–8 represented multiplex PCR reactions performed with varying final concentrations of the three primer pairs (ToLCNDV-DNA-A-F/R, TYLCV-F/R, and ToLCNDV-DNA-B-F/R) as follows: 0.25 μM/0.25 μM, 0.25 μM/0.25 μM and 0.25 μM/0.25 μM; 0.25 μM/0.25 μM, 0.25 μM/0.25 μM and 0.30 μM/0.30 μM; 0.25 μM/0.25 μM, 0.25 μM/0.25 μM and 0.40 μM/0.40 μM; 0.25 μM/0.25 μM, 0.25 μM/0.25 μM and 0.50 μM/0.50 μM; 0.20 μM/0.20 μM, 0.25 μM/0.25 μM and 0.50 μM/0.50 μM; 0.15 μM/0.15 μM, 0.25 μM/0.25 μM and 0.50 μM/0.50 μM; 0.10 μM/0.10 μM, 0.25 μM/0.25 μM and 0.50 μM/0.50 μM; 0.05 μM/0.05 μM, 0.25 μM/0.25 μM and 0.50 μM/0.50 μM; (<b>b</b>) Optimization of annealing temperatures. Lanes 1–6 represent reactions performed at annealing temperatures of 51 °C, 54 °C, 57 °C, 60 °C, 63 °C, 66 °C, respectively. Lane M represents the GeneRuler 1 kb Plus DNA Ladder. Lane CK<sup>−</sup> corresponded to the negative control.</p>
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<p>Sensitivity detection of the multiplex PCR assay was evaluated using serial dilutions of the target plasmid. (<b>a</b>) Sensitivity of a single PCR assay specific for TYLCV was evaluated; (<b>b</b>) Sensitivity of the multiplex PCR specific for ToLCNDV was evaluated; (<b>c</b>) Sensitivity of the multiplex PCR specific for ToLCNDV and TYLCV was evaluated. In all assays, lanes 1–7 represented reactions performed with target plasmid concentrations of 10<sup>6</sup>, 10<sup>5</sup>, 10<sup>4</sup>, 10<sup>3</sup>, 10<sup>2</sup>, and 10<sup>1</sup> copies/μL, respectively. Lane M corresponded to the GeneRuler 1 kb Plus DNA Ladder, and Lane CK<sup>−</sup> represented the negative control.</p>
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<p>The specificity of the multiplex PCR assay for the detection of TYLCV and ToLCNDV was analyzed. Lane 1 represented a positive sample containing a mixture of ToLCNDV and TYLCV. Lane 2 represented a positive sample containing only ToLCNDV. Lane 3 represented a positive sample containing only TYLCV. Lanes 4–10 represented positive samples containing ToLCCNV, TGMV, RAMV, TYLCCNV, TYLCYnV, ToLCTWV, or TbCSV, respectively. Lane M represented the GeneRuler 1 kb Plus DNA Ladder, and Lane CK<sup>−</sup> corresponded to the negative control.</p>
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<p>The multiplex PCR assay in field-collected plant samples. Lanes 1 and 3 represented samples from cucumber. Lane 2 represented samples from watermelon. Lanes 4–10 represented samples from tomato. Lane M represented the GeneRuler 1 kb Plus DNA Ladder, and Lane CK<sup>−</sup> corresponded to the negative control.</p>
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14 pages, 3549 KiB  
Article
Deep Learning-Based Markerless Hand Tracking for Freely Moving Non-Human Primates in Brain–Machine Interface Applications
by Yuhang Liu, Miao Wang, Shuaibiao Hou, Xiao Wang and Bing Shi
Electronics 2025, 14(5), 920; https://doi.org/10.3390/electronics14050920 - 26 Feb 2025
Viewed by 207
Abstract
The motor cortex of non-human primates plays a key role in brain–machine interface (BMI) research. In addition to recording cortical neural signals, accurately and efficiently capturing the hand movements of experimental animals under unconstrained conditions remains a key challenge. Addressing this challenge can [...] Read more.
The motor cortex of non-human primates plays a key role in brain–machine interface (BMI) research. In addition to recording cortical neural signals, accurately and efficiently capturing the hand movements of experimental animals under unconstrained conditions remains a key challenge. Addressing this challenge can deepen our understanding and application of BMI behavior from both theoretical and practical perspectives. To address this issue, we developed a deep learning framework that combines Yolov5 and RexNet-ECA to reliably detect the hand joint positions of freely moving primates at different distances using a single camera. The model simplifies the setup procedure while maintaining high accuracy, with an average keypoint detection error of less than three pixels. Our method eliminates the need for physical markers, ensuring non-invasive data collection while preserving the natural behavior of the experimental subjects. The proposed system exhibits high accuracy and ease of use compared to existing methods. By quickly and accurately acquiring spatiotemporal behavioral metrics, the method provides valuable insights into the dynamic interplay between neural and motor functions, further advancing BMI research. Full article
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<p><b>Block diagram of the overall acquisition.</b> (<b>a</b>) The experimental apparatus consists of a behavior collection cage for non-human primates and a Canon 700D camera for data acquisition. The black lines from I to VII represent seven different acquisition distances, each with a spacing of 20 cm. On VII the camera is 1.8 m away from the acquisition cage. Multiple acquisition distances are used to evaluate the robustness of the system at different distances. (<b>b</b>) It displays a single image in the recognition model, where target detection is performed. (<b>c</b>) It is a joint extraction work for the target detection results. The upper and lower figures show the correspondence between the original image and the joint. (<b>d</b>) It represents 21 random image data corresponding to different collection distances, with decreasing pixel sizes of (640, 480), (560, 420), (480, 360), (400, 300), (320, 240), (240, 180), and (160, 120); a gradual reduction in pixel size is evident in this figure. (<b>e</b>) It showcases detailed hand information extracted from seven randomly chosen images from ‘(<b>d</b>)’, further illustrating the stepwise pixel decrement.</p>
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<p><b>System network model.</b> The model is divided into two parts, namely the target detection module and the posture recognition module. The target detection module uses Yolov5 as the basic model. The model outputs the hand contour after identification and cutting. The data are then input into the second-layer network, RexNet-ECA network, which is applied to the recognition and detection of keypoints. This figure presents a schematic representation of the system model, detailing the data flow from input to output. Through multi-dimensional information detection, multiple hand positions within the image are identified. Subsequent processes extract the joint structure from each detected hand, yielding multiple hand joints within the image. FC, fully connected layer; CSP, stage partial network; SPP, spatial pyramid pooled mud; MBConv, moving inverted bottleneck convolution; Upsample, upsampling; Convolution, convolution layer.</p>
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<p>Specific locations of 21 key marking points on the hand.</p>
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<p><b>System testing.</b> The recognition error and recognizable ratio on reduced pixels (640, 480), (560, 420), (480, 360), (400, 300), (320, 240), (240, 180), and (160, 120) were tested in the view. Five distinct color bars represent the five critical landmarks. The black dots on the folds indicate the average pixel error for each marker point, and the blue dots on the folds indicate the recognition efficiency of the system at different pixel scales, from which it can be seen that the system maintains good recognition efficiency at the first five scales. At resolutions above 320 × 240, it has lower recognition error (average pixel error is less than 3.0) and higher recognition rate (average above 95%).</p>
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<p><b>Video frame analysis.</b> (<b>a</b>) It depicts the system’s recognition of nine frames from a captured video sequence. Distinct colored lines represent different keypoints. The joint extraction map of the hand position at the beginning of the video is marked in (<b>a</b>). The numbers in this figure indicate the position of the center of the hand at different frames. In the video, in frame 5, the monkey grabs the food and retracts the hand into the cage. (<b>b</b>) We utilize the L2 norm to compute the density of the joint keypoints across different frames. The line graph distinctively demarcates two phases: the ‘spread’ and ‘grip’ segments, with clear transitional boundaries, effectively capturing the monkey’s grasping motion.</p>
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<p><b>Gesture classification.</b> The (<b>a</b>,<b>c</b>) views are gesture scatter plots normalized to the reach and grasp gesture data, respectively, with the colored markers representing each of the 21 hand keypoints, from which the reach and hold hand information can be seen more clearly. The (<b>b</b>,<b>d</b>) views are line plots of the reach and grasp gesture joint data, respectively, and the highlighted lines are the data averages for each hand keypoint, showing that the folds all have a strong regularity, indicating that classification can be carried out effectively with these data.</p>
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19 pages, 2451 KiB  
Article
Zebrafish (Danio rerio) Prefer Undisturbed Shoals over Shoals Exposed to the Synthetic Alarm Substance Hypoxanthine-3N-oxide (C5H4N4O2)
by Andrew Velkey, Kaitlyn Kinslow, Megan Bowers, Ethan Hoffman, Jamie Martin and Bandhavi Surisetty
Biology 2025, 14(3), 233; https://doi.org/10.3390/biology14030233 - 25 Feb 2025
Viewed by 584
Abstract
As an anti-predation behavior, shoaling enhances survival among prey species by reducing individual predation risk through mechanisms like the dilution effect and collective vigilance. Zebrafish—a highly social and genetically tractable species—are valuable for studying these behaviors. The present study examined zebrafish’s social preferences [...] Read more.
As an anti-predation behavior, shoaling enhances survival among prey species by reducing individual predation risk through mechanisms like the dilution effect and collective vigilance. Zebrafish—a highly social and genetically tractable species—are valuable for studying these behaviors. The present study examined zebrafish’s social preferences in a 3-chamber open-tank free-swim task, assessing whether visual cues alone could distinguish between an intact and an alarmed shoal exposed to the synthetic alarm substance H3NO. Subjects were allowed to freely associate with either shoal while their behaviors were recorded and analyzed. The results reveal a significant preference for proximity to the intact shoal, indicating zebrafish’s ability to visually discern threat levels. Subjects spent nearly twice as much time in the zone near the intact shoal, with reduced freezing and faster movement velocities compared to the alarmed shoal zone. Males exhibited more freezing behavior than females, consistent with sex-specific strategies in threat response. These findings underscore zebrafish’s reliance on visual cues for social responding under predatory threat and highlight sex-based differences in threat perception. This research expands the understanding of zebrafish’s social dynamics and provides a robust framework for future exploration of the neural mechanisms underlying social behavior and threat assessment in zebrafish. Full article
(This article belongs to the Special Issue Social Behavior in Zebrafish)
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<p>A cartoon illustration of the apparatus depicting the experimental setup. Digital cameras were placed in front of the test tank to capture digital video of the subjects’ movements during their 10 min experimental sessions. The syringe was filled with 5 mL of working solution, and the contents were manually delivered to provide a final concentration of 1.5 nM in the right tank.</p>
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<p>Dot plot for comparison of mean inter-individual distances (IIDs) between alarmed shoals and intact shoals. Error bars represent ±1 SEM.</p>
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<p>An Ethovision heatmap visualization of the subjects’ positions in the center arena. The color intensity ranging from cold (gray) to hot (red) indicates the relative frequency of sampling tracks per location; cool colors (gray to blue) indicate relatively fewer samplings of subjects in that location, while warm to hot colors (yellow/orange to red) indicate relatively more samplings of subjects in that location.</p>
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<p>Comparison of mean percent session time subjects spent in each zone of testing arena. Difference bars between zones are reported using Bonferroni adjustment for family-wise error. Error bars represent ±1 SEM.</p>
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<p>Comparison of mean percent of session time subjects spent in motion in each zone of testing arena. Difference bars between zones are reported using Bonferroni adjustment for family-wise error. Error bars represent ±1 SEM.</p>
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<p>Comparison of mean percent of time subjects spent in motion in zone. Difference bars between zones are reported using Bonferroni adjustment for family-wise error. Error bars represent ±1 SEM.</p>
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<p>Comparison of mean percent session time subjects spent freezing in each zone of testing arena. Difference bars between zones are reported using Bonferroni adjustment for family-wise error. Error bars represent ±1 SEM.</p>
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<p>Comparison of mean percent session times male and females spent freezing in each zone of testing arena. Difference bars between zones are reported using Bonferroni adjustment for family-wise error. Error bars represent ±1 SEM.</p>
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<p>Comparison of mean percent time subjects spent freezing in zone. Difference bars between zones are reported using Bonferroni adjustment for family-wise error. Error bars represent ±1 SEM.</p>
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<p>Comparison of mean velocity during subjects’ movements in each zone of testing arena. Difference bars between zones are reported using Bonferroni adjustment for family-wise error. Error bars represent ±1 SEM.</p>
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20 pages, 512 KiB  
Article
Applying Wearable Sensors and Machine Learning to the Diagnostic Challenge of Distinguishing Parkinson’s Disease from Other Forms of Parkinsonism
by Rana M. Khalil, Lisa M. Shulman, Ann L. Gruber-Baldini, Stephen G. Reich, Joseph M. Savitt, Jeffrey M. Hausdorff, Rainer von Coelln and Michael P. Cummings
Biomedicines 2025, 13(3), 572; https://doi.org/10.3390/biomedicines13030572 - 25 Feb 2025
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Abstract
Background/Objectives: Parkinson’s Disease (PD) and other forms of parkinsonism share motor symptoms, including tremor, bradykinesia, and rigidity. The overlap in their clinical presentation creates a diagnostic challenge, as conventional methods rely heavily on clinical expertise, which can be subjective and inconsistent. This highlights [...] Read more.
Background/Objectives: Parkinson’s Disease (PD) and other forms of parkinsonism share motor symptoms, including tremor, bradykinesia, and rigidity. The overlap in their clinical presentation creates a diagnostic challenge, as conventional methods rely heavily on clinical expertise, which can be subjective and inconsistent. This highlights the need for objective, data-driven approaches such as machine learning (ML) in this area. However, applying ML to clinical datasets faces challenges such as imbalanced class distributions, small sample sizes for non-PD parkinsonism, and heterogeneity within the non-PD group. Methods: This study analyzed wearable sensor data from 260 PD participants and 18 individuals with etiologically diverse forms of non-PD parkinsonism, which were collected during clinical mobility tasks using a single sensor placed on the lower back. We evaluated the performance of ML models in distinguishing these two groups and identified the most informative mobility tasks for classification. Additionally, we examined the clinical characteristics of misclassified participants and presented case studies of common challenges in clinical practice, including diagnostic uncertainty at the patient’s initial visit and changes in diagnosis over time. We also suggested potential steps to address the dataset challenges which limited the models’ performance. Results: Feature importance analysis revealed the Timed Up and Go (TUG) task as the most informative for classification. When using the TUG test alone, the models’ performance exceeded that of combining all tasks, achieving a balanced accuracy of 78.2%, which is within 0.2% of the balanced diagnostic accuracy of movement disorder experts. We also identified differences in some clinical scores between the participants correctly and falsely classified by our models. Conclusions: These findings demonstrate the feasibility of using ML and wearable sensors for differentiating PD from other parkinsonian disorders, addressing key challenges in its diagnosis and streamlining diagnostic workflows. Full article
(This article belongs to the Special Issue Challenges in the Diagnosis and Treatment of Parkinson’s Disease)
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<p>Study workflow. Pksm: non-PD parkinsonism; AUC-ROC: area under the receiver operating characteristic curve.</p>
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<p>Group feature importance for the classification of Parkinson’s disease and non-PD parkinsonism. The ranking of groups is determined based on their importance, which is computed by simultaneously permuting features within each group and measuring the average decrease in accuracy between the original and permuted data. Under the null hypothesis, which posits no association between the group of predictor variables and the model’s prediction, permutation should result in no impact on predictive performance.</p>
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