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13 pages, 11404 KiB  
Essay
The Tectonic Significance of the Mw7.1 Earthquake Source Model in Tibet in 2025 Constrained by InSAR Data
by Shuyuan Yu, Shubi Zhang, Jiaji Luo, Zhejun Li and Juan Ding
Remote Sens. 2025, 17(5), 936; https://doi.org/10.3390/rs17050936 - 6 Mar 2025
Abstract
On 7 January 2025, at Beijing time, an Mw7.1 earthquake occurred in Dingri County, Shigatse, Tibet. To accurately determine the fault that caused this earthquake and understand the source mechanism, this study utilized Differential Interferometric Synthetic Aperture Radar (DInSAR) technology to [...] Read more.
On 7 January 2025, at Beijing time, an Mw7.1 earthquake occurred in Dingri County, Shigatse, Tibet. To accurately determine the fault that caused this earthquake and understand the source mechanism, this study utilized Differential Interferometric Synthetic Aperture Radar (DInSAR) technology to process Sentinel-A data, obtaining the line-of-sight (LOS) co-seismic deformation field for this earthquake. This deformation field was used as constraint data to invert the geometric parameters and slip distribution of the fault. The co-seismic deformation field indicates that the main characteristics of the earthquake-affected area are vertical deformation and east-west extension, with maximum deformation amounts of 1.6 m and 1.0 m for the ascending and descending tracks, respectively. A Bayesian method based on sequential Monte Carlo sampling was employed to invert the position and geometric parameters of the fault, and on this basis, the slip distribution was inverted using the steepest descent method. The inversion results show that the fault has a strike of 189.2°, a dip angle of 40.6°, and is classified as a westward-dipping normal fault, with a rupture length of 20 km, a maximum slip of approximately 4.6 m, and an average slip angle of about −82.81°. This indicates that the earthquake predominantly involved normal faulting with a small amount of left–lateral strike–slip, corresponding to a moment magnitude of Mw7.1, suggesting that the fault responsible for the earthquake was the northern segment of the DMCF (Deng Me Cuo Fault). The slip distribution results obtained from the finite fault model inversion show that this earthquake led to a significant increase in Coulomb stress at both ends of the fault and in the northeastern–southwestern region, with stress loading far exceeding the earthquake triggering threshold of 0.03 MPa. Through analysis, we believe that this Dingri earthquake occurred at the intersection of a “Y”-shaped structural feature where stress concentration is likely, which may be a primary reason for the frequent occurrence of moderate to strong earthquakes in this area. Full article
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Figure 1

Figure 1
<p>Tectonic background of the <span class="html-italic">M</span><sub>w</sub>7.1 Dingri earthquake in 2025. (<b>a</b>) Geographical Location of the Epicenter of the Dingri Earthquake. Black box: research sope of (<b>b</b>); gray focal spheres: source mechanisms of M &gt; 6.5 earthquakes since 1970 as provided by the USGS; Red focal sphere: source mechanism of the Dingri earthquake as given by the USGS. (<b>b</b>) Coverage Area of Earthquake Epicenter Images. Green and white boxes indicate the coverage areas of the European Space Agency’s Sentinel-1A ascending and descending orbits; black box: research sope of (<b>c</b>). (<b>c</b>) Local amplification map of the earthquake-prone area. The red and blue focal spheres represent the source mechanisms provided by the USGS and GCMT, respectively; the yellow dots: the precise aftershock catalog of the <span class="html-italic">M</span><sub>w</sub>7.1 Dingri earthquake [<a href="#B7-remotesensing-17-00936" class="html-bibr">7</a>]; white circles: the county towns in the vicinity of the epicenter; DMCF: Deng Me Cuo Fault; NHF: North Himalayan fault; YLZBF: Yarlung Zangbo River fault; SZDJF: Shenzha–Dingjie fault; TDF: Tangyako–Dingri fault.</p>
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<p>InSAR co-seismic deformation field of the <span class="html-italic">M</span><sub>w</sub>7.1 earthquake in January 2024: (<b>a</b>) T12 ascending track interferogram; (<b>b</b>) T12 ascending track displacement field; (<b>c</b>) T121 descending track interferogram; (<b>d</b>) T121 descending track displacement field; (<b>e</b>) T48 descending track interferogram; (<b>f</b>) T48 descending track displacement field. The red and blue focal spheres represent the source mechanism solutions of the January earthquake determined by the USGS and GCMT, respectively. The black fault traces indicate the identified active fault systems in the study area. One color fringe represents a line-of-sight (LOS) displacement of 50 mm. The red line segment indicates profile AA’, with the profile measurement results shown in <a href="#remotesensing-17-00936-f003" class="html-fig">Figure 3</a>. The red five-pointed star indicates the epicenter provided by the CEA. DMCF: Deng Me Cuo fault; NHF: North Himalayan fault; YLZBF: Yarlung Zangbo River fault; SZDJF: Shenzha–Dingjie fault; TDF: Tangyako–Dingri fault.</p>
Full article ">Figure 2 Cont.
<p>InSAR co-seismic deformation field of the <span class="html-italic">M</span><sub>w</sub>7.1 earthquake in January 2024: (<b>a</b>) T12 ascending track interferogram; (<b>b</b>) T12 ascending track displacement field; (<b>c</b>) T121 descending track interferogram; (<b>d</b>) T121 descending track displacement field; (<b>e</b>) T48 descending track interferogram; (<b>f</b>) T48 descending track displacement field. The red and blue focal spheres represent the source mechanism solutions of the January earthquake determined by the USGS and GCMT, respectively. The black fault traces indicate the identified active fault systems in the study area. One color fringe represents a line-of-sight (LOS) displacement of 50 mm. The red line segment indicates profile AA’, with the profile measurement results shown in <a href="#remotesensing-17-00936-f003" class="html-fig">Figure 3</a>. The red five-pointed star indicates the epicenter provided by the CEA. DMCF: Deng Me Cuo fault; NHF: North Himalayan fault; YLZBF: Yarlung Zangbo River fault; SZDJF: Shenzha–Dingjie fault; TDF: Tangyako–Dingri fault.</p>
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<p>Measurement results of co-seismic deformation profile of <span class="html-italic">M</span><sub>w</sub>7.1 Dingri earthquake in 2025. AA’ deformation field profile is shown in <a href="#remotesensing-17-00936-f002" class="html-fig">Figure 2</a>b,d.</p>
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<p>Estimation of fault geometric parameters. The red line in the histogram and the red dots in the 2D correlation plot indicate the maximum a posteriori solution.</p>
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<p>Inversion model of co-seismic slip distribution based on InSAR data. (<b>a</b>) Three-dimensional co-seismic slip distribution model; (<b>b</b>) surface projection of co-seismic slip distribution. Blue arrows indicate the slip direction.</p>
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<p>Fit of the inversion data for fault slip distribution. (<b>a</b>–<b>c</b>) correspond to the observed values, simulated values, and residuals of the ascending track InSAR data, respectively; (<b>d</b>–<b>f</b>) correspond to the observed values, simulated values, and residuals of the descending track InSAR data, respectively. The red rectangle is the fault plane projected on the surface; the black lines are active faults; DMCF: Deng Me Cuo fault; NHF: North Himalayan fault; SZDJF: Shenzha–Dingjie fault.</p>
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<p>Static ∆CFS in neighboring regions induced by the 2025 Dingri earthquake. (<b>a</b>) ∆CFS at a depth of 5 km underground. (<b>b</b>) ∆CFS at a depth of 7.5 km underground. (<b>c</b>) ∆CFS at a depth of 10 km underground. (<b>d</b>) ∆CFS at a depth of 5 km underground. The green dots the precise aftershock catalog of the <span class="html-italic">M</span><sub>w</sub>7.1 Dingri earthquake [<a href="#B7-remotesensing-17-00936" class="html-bibr">7</a>]; the black lines represent active faults. DMCF: Deng Me Cuo fault; NHF: North Himalayan fault; YLZBF: Yarlung Zangbo River fault; SZDJF: Shenzha–Dingjie Fault; TDF: Tangyako–Dingri Fault.</p>
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20 pages, 5682 KiB  
Article
Gut Metagenome Reveals the Microbiome Signatures in Tibetan and Black Pigs
by Xue Bai, Yiren Gu, Diyan Li and Mingzhou Li
Animals 2025, 15(5), 753; https://doi.org/10.3390/ani15050753 - 6 Mar 2025
Viewed by 63
Abstract
The harsh conditions of the Qinghai–Tibet Plateau pose significant physiological challenges to local fauna, often resulting in gastrointestinal disorders. However, Tibetan pigs have exhibited remarkable adaptability to the high-altitude stress of the Tibetan Plateau, a phenomenon that remains not fully understood in terms [...] Read more.
The harsh conditions of the Qinghai–Tibet Plateau pose significant physiological challenges to local fauna, often resulting in gastrointestinal disorders. However, Tibetan pigs have exhibited remarkable adaptability to the high-altitude stress of the Tibetan Plateau, a phenomenon that remains not fully understood in terms of their gastrointestinal microbiota. This study collected 57 gastrointestinal tract samples from Tibetan pigs (n = 6) and plain black pigs (n = 6) with comparable genetic backgrounds. Samples from the stomach, jejunum, cecum, colon, and rectum, underwent comprehensive metagenomic analysis to elucidate the gut microbiota-related adaptive mechanisms in Tibetan pigs to the extreme high-altitude environment. A predominance of Pseudomonadota was observed within gut microbiome of Tibetan pigs. Significant differences in the microbial composition were also identified across the tested gastrointestinal segments, with 18 genera and 141 species exhibiting differential abundance. Genera such as Bifidobacterium, Megasphaera, Fusobacterium, and Mitsuokella were significantly more abundant in Tibetan pigs than in their lowland counterparts, suggesting specialized adaptations. Network analysis found greater complexity and modularity in the microbiota of Tibetan pigs compared to black pigs, indicating enhanced ecological stability and adaptability. Functional analysis revealed that the Tibetan pig microbiota was particularly enriched with bacterial species involved in metabolic pathways for propionate and butyrate, key short-chain fatty acids that support energy provision under low-oxygen conditions. The enzymatic profiles of Tibetan pigs, characterized by elevated levels of 4-hydroxybutyrate dehydrogenase and glutaconyl-CoA decarboxylase, highlighted a robust fatty acid metabolism and enhanced tricarboxylic acid cycle activity. In contrast, the gut microbiome of plain black pigs showed a reliance on the succinate pathway, with a reduced butyrate metabolism and lower metabolic flexibility. Taken together, these results demonstrate the crucial role of the gastrointestinal microbiota in the adaptation of Tibetan pigs to high-altitude environments by optimizing carbohydrate metabolism and short-chain fatty acid production for efficient energy utilization. This study not only highlights the metabolic benefits conferred by the gut microbiota of Tibetan pigs in extreme environments, but also advances our understanding of the adaptive gastrointestinal mechanisms in plateau-dwelling animals. These insights lay the foundation for exploring metabolic interventions to support health and performance in high-altitude conditions. Full article
(This article belongs to the Section Pigs)
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Figure 1

Figure 1
<p>The intestinal microbial composition of Tibetan pigs and plain black pigs. (<b>A</b>) The overall composition of the top five relative abundances of Tibetan pigs and black pigs at the phylum, class, order, family, genus, and species level. (<b>B</b>–<b>D</b>) The composition of the top five relative abundances of microorganisms in different intestinal segments of Tibetan pigs and black pigs at the phylum (<b>B</b>), genus (<b>C</b>), and species (<b>D</b>) level.</p>
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<p>Distribution of GI microorganisms in Tibetan pigs and plain black pigs across each intestinal segment. (<b>A</b>) Distribution of the top 50 bacterial genera with the highest relative abundance in each GI segment. (<b>B</b>) Correlation heat map at the species level.</p>
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<p>Diversity of intestinal microbial communities of Tibetan pigs and plain black pigs. (<b>A</b>,<b>B</b>) PCoA of samples based on Bray–Curtis distance at the genus levels. (<b>C</b>–<b>E</b>) Alpha diversity comparison based on Shannon diversity indices at the genus levels.</p>
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<p>Differential analysis of GI flora between Tibetan pigs and common black pigs. (<b>A</b>–<b>E</b>) LEfSe differential maps at the genus level across different GI segments in Tibetan pigs and black pigs. (<b>F</b>) Upset map of the distribution of different bacterial genera in each part. (<b>G</b>) Spearman correlation heat map of different bacterial genera, * <span class="html-italic">p</span>  &lt;  0.05, ** <span class="html-italic">p</span>  &lt;  0.01, and *** <span class="html-italic">p</span>  &lt;  0.001. Correlation network diagrams of the GI microbiome in Tibetan pigs (<b>H</b>) and black pigs (<b>I</b>) based on the relative abundance of bacterial genera.</p>
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<p>Differential analysis of the GI flora in Tibetan pigs and black pigs. Heat map showing LEfSe differential results at the species level across different parts of the GI tract in Tibetan and black pigs (LDA &gt; 2, <span class="html-italic">p</span> &lt; 0.05). The coral-red blocks indicate the enrichment of Tibetan pig species, the purple blocks indicate the enrichment of black pig species, and the yellow blocks indicate that neither Tibetan pig nor black pig showed enrichment characteristics in the corresponding parts.</p>
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<p>Functional differences in the GI microbiota of Tibetan pigs and plain black pigs. (<b>A</b>) PCoA diagram of functional genes in the GI microbiota of Tibetan and black pigs based on Bray–Curtis distance. (<b>B</b>) Heat map of differential functions of the GI microbiota based on KEGG pathway enrichment and LEfSe analysis in Tibetan and plain black pigs.</p>
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<p>Functional analysis of bacterial strains in the GI tract of Tibetan pigs. (<b>A</b>) Sangkey diagram of bacterial species significantly enriched in 4–5 GI sites in Tibetan pigs. The name of the bacterial colony and the color of the corresponding flow direction represent the affiliation relationship. (<b>B</b>) Heat map of carbohydrate metabolism in the selected differential bacterial species: blue indicates high enrichment of the bacterial species in the corresponding pathway, while white indicates no enrichment.</p>
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<p>Schematic diagram of the main modules involved in the butyrate metabolic pathway (ko00650). Red indicates significantly higher relative abundance in Tibetan pigs, and green indicates significantly higher abundance in black pigs. Arrows in the figure represent molecular interactions or relationships; circles represent compounds, DNA, and other molecules; rectangles represent gene products (proteins, RNA); and rounded rectangles represent signal pathways.</p>
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32 pages, 6451 KiB  
Article
Calculating the Carbon Footprint of Urban Tourism Destinations: A Methodological Approach Based on Tourists’ Spatiotemporal Behaviour
by Aitziber Pousa-Unanue, Aurkene Alzua-Sorzabal, Roberto Álvarez-Fernández, Alexandra Delgado-Jiménez and Francisco Femenia-Serra
Land 2025, 14(3), 534; https://doi.org/10.3390/land14030534 - 4 Mar 2025
Viewed by 284
Abstract
This study investigates the influence of urban tourists’ behaviour on the environmental performance of a destination, particularly in terms of carbon emissions. Tourist-related emissions are shaped by their choices and behaviours, impacting the overall carbon footprint of the locations they visit. To assess [...] Read more.
This study investigates the influence of urban tourists’ behaviour on the environmental performance of a destination, particularly in terms of carbon emissions. Tourist-related emissions are shaped by their choices and behaviours, impacting the overall carbon footprint of the locations they visit. To assess this impact, we introduce a methodology for quantifying greenhouse gas emissions linked to tourists’ energy consumption. This approach considers key tourism components—activities, accommodation, and transportation—analysing their roles in emissions across a trip’s temporal and spatial dimensions. By integrating tourists’ spatiotemporal behaviour with emissions data, our framework offers insights that can support local climate-responsive urban and tourism policies. We empirically apply the proposed model to the destination of Donostia/San Sebastián (Spain), where the primary travel sequences of visitors are analysed. We utilise cartographic techniques to map the environmental footprints of different tourist profiles, such as cultural and nature tourists. The findings indicate that visitors primarily motivated by nature and outdoor recreation constitute the segment with the highest greenhouse gas emissions (with a minimum footprint of 30.69 kg CO2-equivalent per trip), followed by cultural tourists, and finally, other categories of visitors. The results highlight the practical applications of the proposed model for sustainable tourism management, providing valuable guidance for urban planners and policymakers in mitigating the environmental impacts of tourism. Full article
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<p>Defining visitor flows, trajectories and corridors of a tourist itinerary. Own elaboration, based on Beritelli et al. [<a href="#B9-land-14-00534" class="html-bibr">9</a>].</p>
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<p>Tourists’ key decision chain. Own elaboration.</p>
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<p>Types of itinerary sequences: single vs. combined (direct and indirect) sequence. Own elaboration.</p>
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<p>Donostia: neighbourhood delimitation of the city. Own elaboration, based on Donostiako Udala [<a href="#B101-land-14-00534" class="html-bibr">101</a>].</p>
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<p>Location of main points of interest (POIs) in Donostia. Own elaboration.</p>
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<p>Main tourist corridors in Donostia. Own elaboration based on San Sebastian Turismo [<a href="#B106-land-14-00534" class="html-bibr">106</a>] and San Sebastian City Tour [<a href="#B107-land-14-00534" class="html-bibr">107</a>].</p>
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<p>Location of accommodation clusters in Donostia. Own elaboration.</p>
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<p>Itinerary sequence representation for nature and outdoor recreation tourists: closed path model. Own elaboration.</p>
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<p>Itinerary sequence representation for culture and heritage tourists: closed path sequence. Own elaboration.</p>
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<p>Needed data input for replicating the proposed model in other destinations.</p>
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28 pages, 72675 KiB  
Article
Geochemical and Isotopic Features of Geothermal Fluids Around the Sea of Marmara, NW Turkey
by Francesco Italiano, Heiko Woith, Luca Pizzino, Alessandra Sciarra and Cemil Seyis
Geosciences 2025, 15(3), 83; https://doi.org/10.3390/geosciences15030083 - 1 Mar 2025
Viewed by 217
Abstract
Investigations carried out on 72 fluid samples from 59 sites spread over the area surrounding the Sea of Marmara show that their geochemical and isotopic features are related to different segment settings of the North Anatolian Fault Zone (NAFZ). We collected fluids from [...] Read more.
Investigations carried out on 72 fluid samples from 59 sites spread over the area surrounding the Sea of Marmara show that their geochemical and isotopic features are related to different segment settings of the North Anatolian Fault Zone (NAFZ). We collected fluids from thermal and mineral waters including bubbling and dissolved gases. The outlet temperatures of the collected waters ranged from 14 to 97 °C with no temperature-related geochemical features. The free and dissolved gases are a mixture of shallow and mantle-derived components. The large variety of geochemical features comes from intense gas–water (GWI) and water–rock (WRI) interactions besides other processes occurring at relatively shallow depths. CO2 contents ranging from 0 to 98.1% and helium isotopic ratios from 0.11 to 4.43 Ra indicate contributions, variable from site to site, of mantle-derived volatiles in full agreement with former studies on the NAFZ. We propose that the widespread presence of mantle-derived volatiles cannot be related only to the lithospheric character of the NAFZ branches and magma intrusions have to be considered. Changes in the vertical permeability induced by fault movements and stress accumulation during seismogenesis, however, modify the shallow/deep ratio of the released fluids accordingly, laying the foundations for future monitoring activities. Full article
(This article belongs to the Section Geochemistry)
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Figure 1
<p>Map of historic earthquakes in the wider Marmara region compiled from various sources [<a href="#B17-geosciences-15-00083" class="html-bibr">17</a>,<a href="#B18-geosciences-15-00083" class="html-bibr">18</a>,<a href="#B19-geosciences-15-00083" class="html-bibr">19</a>,<a href="#B20-geosciences-15-00083" class="html-bibr">20</a>]. Labels indicate the year of the event for magnitudes M ≥ 7. White lines depict active faults according to the General Directorate of Mineral Research and Exploration (MTA) [<a href="#B21-geosciences-15-00083" class="html-bibr">21</a>]; off-shore faults are taken from Armijo et al. (2002) [<a href="#B14-geosciences-15-00083" class="html-bibr">14</a>]. Orange and red lines indicate the ruptures related to the Ganos earthquake of 1912 and the Izmit/Düzce events of 1999, respectively.</p>
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<p>Map of fluid sampling sites around the Sea of Marmara. Symbols indicate color-coded water temperatures. Small white circles depict sites with bubbling gases. Values are sample numbers used in this study (see <a href="#geosciences-15-00083-t001" class="html-table">Table 1</a>). Names of geographic areas investigated are given.</p>
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<p>Piper diagram of the water samples as a function of the geographical areas. Sample labels as the ID numbers in <a href="#geosciences-15-00083-t002" class="html-table">Table 2</a>.</p>
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<p>Ca vs Mg (<b>a</b>) and HCO<sub>3</sub> (<b>b</b>). The occurrence of GWI processes allows CO<sub>2</sub> dissolution that is responsible for the observed geochemical features related to WRI resulting in dolomite and calcite dissolution to various extents. Sample labels are the same as the ID numbers in <a href="#geosciences-15-00083-t002" class="html-table">Table 2</a>.</p>
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<p>Na vs HCO<sub>3</sub> (<b>a</b>) and Na vs. Cl (<b>b</b>). The occurrence of WRI and GWI processes is responsible for the observed geochemical features. Blue star symbol = sea water. Sample labels are the same as the ID numbers in <a href="#geosciences-15-00083-t002" class="html-table">Table 2</a>. Symbol colors are as shown in <a href="#geosciences-15-00083-f003" class="html-fig">Figure 3</a>.</p>
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<p>Ca-SO<sub>4</sub> plot showing that gypsum dissolution is not the main process responsible for the SO<sub>4</sub> ions, with the water chemistry being a consequence of WRI and GWI processes. Sample labels are the same as the ID numbers in <a href="#geosciences-15-00083-t002" class="html-table">Table 2</a>. SW = sea water.</p>
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<p>δ<sup>18</sup>O–δD plot for the collected waters. Samples fall between the two reference lines representing the EMMWL (Eastern Mediterranean Meteoric Water Line; Hatvani et al., 2023 [<a href="#B62-geosciences-15-00083" class="html-bibr">62</a>]) and the GMWL (Global Meteoric Water Line; Rozanski et al., 1993 [<a href="#B63-geosciences-15-00083" class="html-bibr">63</a>]). BMWL refers to the Bursa local meteoric water line proposed by Imbach et al. (1997) [<a href="#B38-geosciences-15-00083" class="html-bibr">38</a>]. Sample labels are the same as the ID numbers in <a href="#geosciences-15-00083-t002" class="html-table">Table 2</a>.</p>
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<p>CO<sub>2</sub>-N<sub>2</sub> relationships for bubbling (filled circles) and dissolved (diamond) gases indicating the presence of two end members in the gas phase, namely the shallow atmospheric-derived N<sub>2</sub> component and the deep-originated CO<sub>2</sub>, vented over the Marmara area that mix at variable extents. Numbers indicate the sample IDs as in <a href="#geosciences-15-00083-t001" class="html-table">Table 1</a>.</p>
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<p>CO<sub>2</sub>-CH<sub>4</sub>-N<sub>2</sub> triangular diagram of the bubbling (filled circles) and dissolved (diamonds) gases showing the relative contents of the three end members N<sub>2</sub>, CO<sub>2</sub> and CH<sub>4</sub>. We plotted the N<sub>2</sub> excess with respect to the atmospheric nitrogen. The arrows highlight the GWI processes (CO<sub>2</sub> loss and increased N<sub>2</sub> and CH<sub>4</sub> contents) as well as mixings due to CO<sub>2</sub> addition from various sources that significantly changed the composition of the pristine gas phase. The numbers beside the symbols indicate the site as listed in <a href="#geosciences-15-00083-t001" class="html-table">Table 1</a>.</p>
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<p>CO<sub>2</sub> content vs δ<sup>13</sup>C<sub>CO2</sub> for the bubbling gases (<b>a</b>) and for δ<sup>13</sup>C<sub>TDIC</sub> of the dissolved gases (<b>b</b>). The plots depict a clear direct correlation between isotopic ratios and CO<sub>2</sub> and HCO<sub>3</sub> contents. The contemporary trends denote the fractionation with quantitative loss of gaseous CO<sub>2</sub> and its heavy isotope as well as the occurrence of further fractionation processes. The occurrence of similar trends followed by samples from different sites around the Marmara area suggests that the vented CO<sub>2</sub> is not solely controlled by shallow interactions with groundwaters, and that the coexistence of multiple sources has to be considered.</p>
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<p>Helium isotopic ratios (uncorrected R/Ra values) and <sup>4</sup>He/<sup>20</sup>Ne relationships for both dissolved and bubbling gases. The theoretical lines represent binary mixing trends of atmospheric helium with mantle-originated and crustal helium. The assumed end members for He-isotopic ratios and <sup>4</sup>He/<sup>20</sup>Ne ratios are ASW (1 Ra, He/Ne = 0.267: Holocer et al., 2002) [<a href="#B49-geosciences-15-00083" class="html-bibr">49</a>]; 8Ra for a MORB-type mantle; and 3.5 Ra for contaminated mantle; crust 0.05Ra and <sup>4</sup>He/<sup>20</sup>Ne ratio = 10,000. Filled circles = bubbling gases; filled diamonds = dissolved gases. Sample IDs are as reported in <a href="#geosciences-15-00083-t003" class="html-table">Table 3</a>. All error bars are within the symbol size.</p>
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<p>CO<sub>2</sub>/<sup>3</sup>He–<sup>4</sup>He. The plot shows how the vented gases are a mixture of two main components: magmatic-type and crustal-originated. Circles = bubbling gases; diamonds = dissolved gases. The arrows display the main trends affecting the composition of the gas phase.</p>
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<p>Map showing locations mentioned in the text. Numbers refer to sampling sites of this study (see <a href="#geosciences-15-00083-t001" class="html-table">Table 1</a>).</p>
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<p>Chemical composition of thermal and mineral waters around the Sea of Marmara. The diameter of the pies scales with the specific electrical conductivity of the waters. Small circles in the centre of the pies indicate the water temperature: blue—cold (&lt;20 °C); orange—hot (&gt;40 °C).</p>
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<p>Gas composition of thermal and mineral waters around the Sea of Marmara. Small white circles in the centre of the pies indicate bubbling gases.</p>
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<p>Helium isotope ratios given in R/Ra at mineral and thermal waters around the Sea of Marmara. Light purple areas depict Tertiary volcanic rocks, hatched areas mark intrusive igneous rocks of Paleozoic to Cenozoic age. Light and dark gray areas indicate Mesozoic and Paleozoic rocks, respectively. White areas are Paleogene to Quaternary sediments. Simplified geology modified from Pawlewicz et al. (1997) [<a href="#B83-geosciences-15-00083" class="html-bibr">83</a>].</p>
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18 pages, 5285 KiB  
Article
CCR2 Regulates Referred Somatic Hyperalgesia by Mediating T-Type Ca2+ Channel Currents of Small-Diameter DRG Neurons in Gastric Ulcer Mice
by Ziyan Yuan, Huanhuan Liu, Zhijun Diao, Wei Yuan, Yuwei Wu, Simeng Xue, Xinyan Gao and Haifa Qiao
Brain Sci. 2025, 15(3), 255; https://doi.org/10.3390/brainsci15030255 - 27 Feb 2025
Viewed by 168
Abstract
Background: Referred pain frequently co-exists with visceral pain. However, the exact mechanism governing referred somatic hyperalgesia remains elusive. Methods: By injecting 20% acetic acid into the stomach, we established a mouse model of gastric ulcer (GU). Hematoxylin and eosin (H&E) staining [...] Read more.
Background: Referred pain frequently co-exists with visceral pain. However, the exact mechanism governing referred somatic hyperalgesia remains elusive. Methods: By injecting 20% acetic acid into the stomach, we established a mouse model of gastric ulcer (GU). Hematoxylin and eosin (H&E) staining was used as the evaluation criterion for the gastric ulcer model. Evan’s blue (EB) and von Frey tests detected the somatic sensitized area. The DRG neurons distributed among the spinal segments of the sensitized area were prepared for biochemical and electrophysiological experiments. The CCR2 antagonist was intraperitoneally (i.p.) injected into GU mice to test the effect of blocking CCR2 on somatic neurogenic inflammation. Results: GU not only instigated neurogenic plasma extravasation and referred somatic allodynia in the upper back regions spanning the T9 to T11 segments but also augmented the co-expression of T-type Ca2+ channels and CCR2 and led to the gating properties of T-type Ca2+ channel alteration in T9–T11 small-diameter DRG neurons. Moreover, the administration of the CCR2 antagonist inhibited the T-type Ca2+ channel activation, consequently mitigating neurogenic inflammation and referred somatic hyperalgesia. The application of the CCR2 agonist to normal T9–T11 small-diameter DRG neurons simulates the changes in the gating properties of T-type Ca2+ channel that occur in the GU group. Conclusions: Therefore, these findings indicate that CCR2 may function as a critical regulator in the generation of neurogenic inflammation and mechanical allodynia by modulating the gating properties of the T-type Ca2+ channels. Full article
(This article belongs to the Special Issue Recent Advances in Pain Research)
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<p>Pathological changes in the gastric ulcer (GU) mice induced by acetic acid. (<b>A</b>) Experimental process diagram. (<b>B</b>) Representative images of H&amp;E staining of the stomach in control and GU mice. The right panels provide higher-magnification pictures of the boxed regions in the left panels [left: 10× (magnified), scale bar, 250 μm; right: 20× (magnified), scale bar, 100 μm] (n = 6 mice per group). (<b>C</b>,<b>F</b>) Representative images of EB plasma extravasation points scattered in the regions of operative incision and upper back following GU, as compared to the control. (<b>D</b>,<b>G</b>) Schematic representation of EB points in the skin from control and GU mice. (<b>E</b>,<b>H</b>) Schematics of merged EB points in the skin from the control and GU group. (<b>I</b>) The number of EB points in the dermatomes of the T2-L2 spinal segments. (<b>J</b>) Quantification of total EB points in two groups (n = 6 mice per group). (<b>K</b>) Withdrawal threshold to mechanical stimulation in the T9–T11 upper-back exudation points of each group (n = 6 mice per group). The red arrows indicate the locations of EB points, and the blue dots represent the EB points. Compared with the control group, **** <span class="html-italic">p</span> &lt; 0.0001.</p>
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<p>Both the expression and gating properties of the T-type channel in small-diameter DRG neurons are increased following acetic acid-induced GU. (<b>A</b>) Representative IHC images of DAPI (blue) and T-type Ca<sup>2+</sup> channels (Ca<sub>v</sub>3.2) (green) in DRGs of control and GU groups. The right panels provide higher-magnification pictures of the boxed regions in the left panels [left: 10× (magnified), scale bar, 250 μm; right: 40× (magnified), scale bar, 75 μm]. The numbers of Ca<sub>v</sub>3.2-positive DRG neurons are shown in two groups (n = 12 DRGs from eight mice per group). Blue represents the cell nucleus, and green represents the T-type (Ca<sub>v</sub>3.2)-positive neurons. (<b>B</b>) Representative 40× (magnified) images of recording from a small-diameter (&lt;20 μm) and medium-diameter (20–30  μm)  DRG neurons in a whole-mount DRG preparation are recorded [scale bar, 10 μm]. The dashed red circles represent the morphology of the clamped cells. (<b>C</b>) Top: Representative traces of T-type channel activation curves from different diameter DRG neurons in two groups. Bottom: The voltage protocol used to activate the T-type channels. (<b>D</b>,<b>E</b>) An overview of the normalized (pA/pF) <span class="html-italic">I<sub>T-type</sub></span> density versus voltage relationship from DRG small-diameter (<b>D</b>) and medium-dimeter (<b>E</b>) neurons [two-way RM ANOVA with multiple comparisons tests: small-diameter DRG neurons, F <sub>(1, 11)</sub> = 11.53, <span class="html-italic">p</span> = 0.0060; medium-diameter DRG neurons, F <sub>(1, 16)</sub> = 0.1683, <span class="html-italic">p</span> = 0.6870]. (<b>F</b>–<b>I</b>) Boltzmann fits for normalized conductance, G/G<sub>max</sub>, voltage relations for voltage-dependent activation (<b>F</b>,<b>H</b>), and inactivation (<b>G</b>,<b>I</b>) of small- and medium-diameter DRG neurons in two groups [two-way RM ANOVA with multiple comparisons tests: small-diameter DRG neurons, activation curves: F <sub>(1, 11)</sub> = 6.172, <span class="html-italic">p</span> = 0.0303; inactivation curves: F <sub>(1, 22)</sub> = 0.2834, <span class="html-italic">p</span> = 0.5998; medium-diameter DRG neurons, activation curves: F <sub>(1, 16)</sub> = 0.01542, <span class="html-italic">p</span> = 0.9027; inactivation curves: F <sub>(1, 21)</sub> = 1.001, <span class="html-italic">p</span> = 0.3284]. (Small-diameter DRG neurons: control, n = 5–12 cells from five mice, GU, n = 8–12 from five mice; medium-diameter DRG neurons: control, n = 10–12 cells from five mice, GU, n = 8–12 from four mice.). Compared with the control group, * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>Effects of inhibition of the chemokine CCR2 on <span class="html-italic">I<sub>T-type</sub></span> and referred somatic hyperalgesia in gastric ulcer mice. (<b>A</b>) Representative IHC images of DAPI (blue), T-type Ca<sup>2+</sup> channels (green), and CCR2 (red) in DRGs of control and GU model. The right panels provide higher-magnification pictures of the boxed regions in the left panels [left: 10× (magnified), scale bar, 250 μm; right: 40× (magnified), scale bar, 75 μm]. The numbers of T-type (Ca<sub>v</sub>3.2)<sup>+</sup> and CCR2<sup>+</sup> DRG neurons are shown in two groups (n = 12 DRGs from eight mice per group). Blue represents the cell nucleus, green represents the T-type (Ca<sub>v</sub>3.2)-positive neurons and red represents the CCR2-positive neurons. (<b>B</b>) The schematic shows drugs and CCR2 antagonists added to the bath. (<b>C</b>) Representative 40× (magnified) image of recording from a small-diameter (&lt;20  μm) DRG neurons in a whole-mount DRG preparation is recorded [scale bar, 10 μm]. The dashed red circles represent the morphology of the clamped cell. (<b>D</b>) Top: Representative traces of T-type channel activation curves from small-diameter DRG neurons treated with vehicle or RS102895 in GU mice. Bottom: The voltage protocol is used to activate the T-type channels. (<b>E</b>) An overview of the normalized (pA/pF) <span class="html-italic">I<sub>T-type</sub></span> density versus voltage relationship from DRG small-diameter neurons [two-way RM ANOVA with multiple comparisons tests: <span class="html-italic">F</span> <sub>(1, 13)</sub> = 8.014, <span class="html-italic">p</span> = 0.0142]. (<b>F</b>) Boltzmann fits for normalized conductance (G/G<sub>max</sub>) and voltage-dependent activation. (<b>G</b>) Boltzmann fits for voltage-dependent inactivation [two-way RM ANOVA withmultiple comparisons tests: activation curves, <span class="html-italic">F</span> <sub>(1, 13)</sub> = 3.154, <span class="html-italic">p</span> = 0.1011; inactivation curves, <span class="html-italic">F</span> <sub>(1, 22)</sub> = 0.006003, <span class="html-italic">p</span> = 0.9391] (vehicle, n = 8–12 cells from five mice; RS102895, n = 6–9 cells from four mice). (<b>H</b>) Quantification of total EB points in each group. (<b>I</b>) Withdrawal threshold to mechanical stimulation in the T9–T11 upper-back exudation points of each group (n = six mice per group). (<b>J</b>,<b>K</b>) Representative images on the left showing EB plasma extravasation points after i.p. of vehicle (<b>J</b>) or i.p. of RS102895; (<b>K</b>) 100 μL. Schematic representations of EB sites on the body surface of i.p. of vehicle (<b>J</b>) or i.p. of RS102895 (<b>K</b>) are on the right (n = six mice per group). The red arrows indicate the locations of EB points, and the blue dots represent the EB points. Compared with the vehicle group, * <span class="html-italic">p</span> &lt; 0.05, *** <span class="html-italic">p</span> &lt; 0.001, **** <span class="html-italic">p</span> &lt; 0.0001.</p>
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20 pages, 8319 KiB  
Article
Shortening the Saturation Time of PBAT Sheet Foaming via the Pre-Introducing of Microporous Structures
by Fangwei Tian, Junjie Jiang, Yaozong Li, Hanyi Huang, Yushu Wang, Ziwei Qin and Wentao Zhai
Materials 2025, 18(5), 1044; https://doi.org/10.3390/ma18051044 - 26 Feb 2025
Viewed by 236
Abstract
Poly (butylene adipate-co-terephthalate) (PBAT) foam sheets prepared by foaming supercritical fluids are characterized by high resilience, homogeneous cellular structure, and well-defined biodegradability. However, the inert chemical structure and the rigid hard segments restrict the diffusion of CO2 within the PBAT matrix, resulting [...] Read more.
Poly (butylene adipate-co-terephthalate) (PBAT) foam sheets prepared by foaming supercritical fluids are characterized by high resilience, homogeneous cellular structure, and well-defined biodegradability. However, the inert chemical structure and the rigid hard segments restrict the diffusion of CO2 within the PBAT matrix, resulting in extremely long gas saturation times as long as 9 h at a thickness of 12 mm. In this study, microporous structures were pre-introduced into the PBAT matrix to provide a fast gas diffusion pathway during the saturation process. After 2 h of saturation, PBAT foam sheets with expansion ratio of 10 to 13.8 times were prepared. The interaction of CO2 with PBAT was systematically investigated, and the CO2 sorption process was evaluated kinetically and thermodynamically using the Fickian diffusion theory. The solubility and diffusion rate of CO2 in pretreated PBAT sheets with different microporous sizes and densities were investigated, and the effects of pretreatment strategies on the foaming behavior and cell structure of PBAT foam sheets were discussed. The introduction of a microporous structure not only reduces saturation time but also enhances solubility, enabling the successful preparation of soft foams with high expansion ratios and resilience. After undergoing foaming treatment, the PBAT pretreated sheets with a 10 μm microporous structure and a density of 0.45 g/cm3 demonstrated improved mechanical properties: their hardness decreased to 35 C while resilience increased to 58%, reflecting enhanced elastic recovery capabilities. The pretreatment method, which increases the diffusion rate of CO2 in PBAT sheets, offers a straightforward approach that provides valuable insights into achieving rapid and efficient foaming of thick PBAT sheets in industrial applications. Full article
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<p>Schematic of PBAT foam preparation. (<b>a</b>) One-step foaming of PBAT sheets; (<b>b</b>) foaming of PBAT pretreated sheets; (<b>c</b>) schematic diagram of the PBAT sheet pretreatment–short-duration foaming process; (<b>d</b>) parameter distributions during pretreatment–short-duration saturated foaming process.</p>
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<p>CO<sub>2</sub> sorption and diffusion in untreated PBAT sheets. (<b>a</b>) Schematic diagram of CO<sub>2</sub> diffusion in PBAT matrix; (<b>b</b>) CO<sub>2</sub> sorption in PBAT sheets of different thicknesses at 100 °C—18 MPa; (<b>c</b>) fitting of Fick’s diffusion model; (<b>d</b>) isothermal sorption of CO<sub>2</sub> in PBAT; (<b>e</b>) kinetic linear fitting of diffusion coefficients at different temperatures; and (<b>f</b>) kinetic linear fitting of diffusion coefficients at different pressures.</p>
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<p>PBAT foam thermal behavior. (<b>a</b>) DSC curves of the PBAT samples treated under various pressures and temperatures; (<b>b</b>) degree of crystallinity based on DSC pattern.</p>
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<p>SEM image of the evolution of the cell structure of untreated PBAT sheet (6 mm thickness) with saturation time at 100 °C—18 MPa. (<b>a<sub>1</sub></b>–<b>e<sub>1</sub></b>) evolution of the cell structure in the edge region with saturation time (10–150 min); (<b>a<sub>2</sub></b>–<b>e<sub>2</sub></b>) evolution of the cell structure in the middle region with saturation time (10–150 min); and (<b>a<sub>3</sub></b>–<b>e<sub>3</sub></b>) evolution of the cell structure in the core region with saturation time (10–150 min).</p>
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<p>Dynamic information on the cell structure of PBAT foams obtained at 100 °C—18 MPa. (<b>a</b>) Cell size across different positions at different times; (<b>b</b>) cell density across different positions at different times.</p>
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<p>SEM image of microporous structures of typical PBAT pretreated sheets. (<b>a<sub>1</sub></b>–<b>c<sub>1</sub></b>) samples with different microporous sizes at a density of 0.35 g/cm<sup>3</sup>; (<b>a<sub>2</sub></b>–<b>c<sub>2</sub></b>) samples with different microporous sizes at a density of 0.45 g/cm<sup>3</sup>; (<b>a<sub>3</sub></b>–<b>c<sub>3</sub></b>) samples with different microporous sizes at a density of 0.65 g/cm<sup>3</sup>.</p>
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<p>Diffusive behavior of CO<sub>2</sub> in pretreated PBAT sheets. (<b>a</b>) Effect of microporous structure on cell wall; (<b>b</b>) CO<sub>2</sub> sorption process under different microporous structures; (<b>c</b>) linear fitting of diffusion coefficients; (<b>d</b>) schematic diagram of rapid CO<sub>2</sub> diffusion in pretreated PBAT sheets.</p>
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<p>SEM images of foamed PBAT sheets with different microporous structures (red circles show graded cell structures). (<b>a<sub>1</sub></b>–<b>c<sub>1</sub></b>) samples with different micropore sizes of 10 μm, 75 μm and 140 μm at a density of 0.35 g/cm<sup>3</sup>; (<b>a<sub>2</sub></b>–<b>c<sub>2</sub></b>) samples with different micropore sizes of 10 μm, 75 μm and 140 μm at a density of 0.35 g/cm<sup>3</sup>; (<b>a<sub>3</sub></b>–<b>c<sub>3</sub></b>) samples with different micropore sizes of 10 μm, 75 μm and 140 μm at a density of 0.35 g/cm<sup>3</sup>.</p>
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<p>Cell morphology of PBAT foamed sheets with different microporous structures. (<b>a</b>) Cell size; (<b>b</b>) cell density; (<b>c</b>) expansion ratio.</p>
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<p>Mechanical properties of PBAT foams. (<b>a</b>,<b>b</b>) Compression properties, (<b>c</b>) hardness, and (<b>d</b>) resilience.</p>
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17 pages, 24593 KiB  
Article
Enhanced PolSAR Image Segmentation with Polarization Channel Fusion and Diffusion-Based Probability Modeling
by Hao Chen, Yuzhuo Hou, Xiaoxiao Fang and Chu He
Electronics 2025, 14(4), 791; https://doi.org/10.3390/electronics14040791 - 18 Feb 2025
Viewed by 210
Abstract
With the advancement of polarimetric synthetic aperture radar (PolSAR) imaging technology and the growing demand for image interpretation, extracting meaningful land cover information from PolSAR images has become a key research focus. To address the segmentation challenge, we propose an innovative method. First, [...] Read more.
With the advancement of polarimetric synthetic aperture radar (PolSAR) imaging technology and the growing demand for image interpretation, extracting meaningful land cover information from PolSAR images has become a key research focus. To address the segmentation challenge, we propose an innovative method. First, features from co-polarization and cross-polarization channels are separately used as dual inputs, and a cross-attention mechanism effectively fuses these features to capture correlations between different polarization information. Second, a diffusion framework is employed to jointly model target features and class probabilities, aiming to improve segmentation accuracy by learning and fitting the probabilistic distribution of target labels. Finally, experimental results demonstrate that the proposed method achieves superior performance in PolSAR image segmentation, effectively managing complex polarization relationships while offering robustness and broad application potential. Full article
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<p>PolSAR image segmentation framework.</p>
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<p>Conditional diffusion for image segmentation.</p>
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<p>Hybrid modeling framework for PolSAR segmentation.</p>
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<p>Dual-path polarization channel feature fusion module by cross attention (DCFM).</p>
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<p>The used PolSAR datasets.</p>
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<p>Segmentation rResults of the SanALOS2 dataset. (<b>a</b>) Ground truth. (<b>b</b>) FCNs. (<b>c</b>) PSPNet. (<b>d</b>) EmaNet. (<b>e</b>) DANet. (<b>f</b>) SETR. (<b>g</b>) Segformer. (<b>h</b>) Proposal.</p>
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<p>Segmentation results of the HainanC dataset. (<b>a</b>) Ground truth. (<b>b</b>) FCNs. (<b>c</b>) PSPNet. (<b>d</b>) EmaNet. (<b>e</b>) DANet. (<b>f</b>) SETR. (<b>g</b>) Segformer. (<b>h</b>) Proposal.</p>
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<p>OA curves of different methods in the training process on the Hainan dataset.</p>
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13 pages, 1586 KiB  
Article
Non-Hospitalized Long COVID Patients Exhibit Reduced Retinal Capillary Perfusion: A Prospective Cohort Study
by Clayton E. Lyons, Jonathan Alhalel, Anna Busza, Emily Suen, Nathan Gill, Nicole Decker, Stephen Suchy, Zachary Orban, Millenia Jimenez, Gina Perez Giraldo, Igor J. Koralnik and Manjot K. Gill
J. Imaging 2025, 11(2), 62; https://doi.org/10.3390/jimaging11020062 - 17 Feb 2025
Viewed by 2716
Abstract
The mechanism of post-acute sequelae of SARS-CoV-2 (PASC) is unknown. Using optical coherence tomography angiography (OCT-A), we compared retinal foveal avascular zone (FAZ), vessel density (VD), and vessel length density (VLD) in non-hospitalized Neuro-PASC patients with those in healthy controls in an effort [...] Read more.
The mechanism of post-acute sequelae of SARS-CoV-2 (PASC) is unknown. Using optical coherence tomography angiography (OCT-A), we compared retinal foveal avascular zone (FAZ), vessel density (VD), and vessel length density (VLD) in non-hospitalized Neuro-PASC patients with those in healthy controls in an effort to elucidate the mechanism underlying this debilitating condition. Neuro-PASC patients with a positive SARS-CoV-2 test and neurological symptoms lasting ≥6 weeks were included. Those with prior COVID-19 hospitalization were excluded. Subjects underwent OCT-A with segmentation of the full retinal slab into the superficial (SCP) and deep (DCP) capillary plexus. The FAZ was manually delineated on the full slab in ImageJ. An ImageJ macro was used to measure VD and VLD. OCT-A variables were analyzed using linear mixed-effects models with fixed effects for Neuro-PASC, age, and sex, and a random effect for patient to account for measurements from both eyes. The coefficient of Neuro-PASC status was used to determine statistical significance; p-values were adjusted using the Benjamani–Hochberg procedure. Neuro-PASC patients (N = 30; 60 eyes) exhibited a statistically significant (p = 0.005) reduction in DCP VLD compared to healthy controls (N = 44; 80 eyes). The sole reduction in DCP VLD in Neuro-PASC may suggest preferential involvement of the smallest blood vessels. Full article
(This article belongs to the Section Medical Imaging)
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<p>Optical coherence tomography angiography (OCT-A) image processing in Image J. (<b>A</b>) Original image of superficial capillary plexus (SCP) slab prior to processing. (<b>B</b>) Resulting large vessel mask after binarization with maximum entropy plug-in. (<b>C</b>) Binarized SCP slab used to calculate vessel density (VD) with the removed large vessels. (<b>D</b>) Final binarized and skeletonized SCP slab used to calculate vessel length density (VLD) with removal of the large vessels.</p>
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<p>Quality of life and cognitive function results in non-hospitalized post-acute sequelae SARS-CoV-2 infection (PASC) patients with predominantly neurologic symptoms. Neuro-PASC patients exhibited a broad reduction in quality of life compared to a US normative population and significantly worse cognitive function in attention only. * <span class="html-italic">p</span> &lt; 0.05.</p>
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<p>Deep capillary plexus (DCP) VLD in Neuro-PASC patients versus healthy controls. A box plot showing the difference in DCP VLD between healthy controls and Neuro-PASC patients.</p>
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28 pages, 19915 KiB  
Article
Comprehensive Analysis of Hormonal Signaling Pathways and Gene Expression in Flesh Segment Development of Chinese Bayberry (Myrica rubra)
by Yihan Fu, Shuwen Zhang, Li Yang, Yu Zong, Yongqiang Li, Xingjiang Qi, Wenrong Chen, Fanglei Liao and Weidong Guo
Plants 2025, 14(4), 571; https://doi.org/10.3390/plants14040571 - 13 Feb 2025
Viewed by 436
Abstract
Chinese bayberry (Myrica rubra or Morella rubra) is a valuable fruit, yet the mechanism of its flesh segment development is not well understood. Using paraffin sectioning, we investigated the flower buds of the ‘Biqi’ and ‘Zaojia’ varieties, revealing that the flesh [...] Read more.
Chinese bayberry (Myrica rubra or Morella rubra) is a valuable fruit, yet the mechanism of its flesh segment development is not well understood. Using paraffin sectioning, we investigated the flower buds of the ‘Biqi’ and ‘Zaojia’ varieties, revealing that the flesh segment development in these Chinese bayberry varieties involved the formation of a primordium outside the ovary wall, the establishment of a simple columnar structure, and the formation of the primary flesh segment. Assessment of endogenous hormone levels indicated the significant reductions in jasmonic acid (JA) and indole-3-acetic acid (IAA) levels at the critical stages of flesh segment development. Correlation analysis highlighted the essential roles of IAA, JA, abscisic acid (ABA), and gibberellins in the flesh segment developmental process, underscoring the complex interactions driven primarily by the IAA, JA, and ABA networks. Gene modules positively correlated with flesh segment development were identified using transcriptome-based weighted gene co-expression network analysis (WGCNA). Differentially expressed genes (DEGs) were enriched in plant hormone signal transduction pathways, particularly for upregulated genes associated with auxin and JA signaling. Key genes predicted to be involved in flesh segment development included LAX2 and LAX3 (auxin transport), JAZ6 (JA signaling repression), and KAN1 and KAN4 (regulating multiple hormonal signaling pathways). Quantitative real-time polymerase chain reaction (qRT-PCR) validation confirmed that the expression trends for these genes were consistent across both varieties, particularly for CRC, SEP1, SEP3, IAA7, and JAZ6. Immunofluorescence localization studies revealed that auxin was primarily distributed in the central vascular bundle and outer cells of the flesh segment. This uneven auxin distribution might contribute to the unique morphology of flesh segments. Overall, this study provides insights into the hormonal regulation and genetic factors involved in the development of Chinese bayberry flesh segments. Full article
(This article belongs to the Section Plant Development and Morphogenesis)
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<p>Comparisons of (<b>a</b>) bayberry, (<b>b</b>) cherry, and (<b>c</b>) citrus fruit structures. Bayberry has a unique fruit structure where the outer edge of the pericarp is specialized to form flesh segments. These segments are morphologically similar to the juice sacs of citrus.</p>
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<p>Morphological and histological characteristics of bayberry flower buds. The bayberry variety used for the morphological and histological observations was ‘Biqi’. The flower buds had not reached the peak flowering stage during these observations. (<b>a</b>) The longitudinal section of the inflorescence in the bayberry flower buds. (<b>b</b>) Paraffin sections showing the development of bayberry flower buds: 1 represents the pistil primordia of the flower bud, 2 represents the flower bud bracts, 3 represents the bract primordia, 4 represents the pistil, 5 represents the stigma, and 6 represents the ovary.</p>
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<p>Paraffin sections illustrating the development of bayberry flesh segments. For the ‘Biqi’ variety, the first column shows the appearance of flower buds sampled at different time points (scale bar = 2 mm). (<b>A</b>–<b>D</b>) display flower bud sections under a 20× magnification (scale bar = 200 μm), while (<b>A’</b>–<b>D’</b>) provide observations of the red-boxed areas in (<b>A</b>–<b>D</b>) under a 40× magnification (scale bar = 40 μm), with the arrows indicating the protruding structure of the flesh segment primordia or primary flesh segment. For the ‘Zaojia’ variety, the first column shows the appearance of flower buds sampled at different time points (scale bar = 2 mm). (<b>E</b>–<b>H</b>) show flower bud sections under a 20× magnification (scale bar = 200 μm), while (<b>E’</b>–<b>H’</b>) provide observations of the red-boxed areas in (<b>E</b>–<b>H</b>) under a 40× magnification (scale bar = 40 μm), with the arrows indicating the protruding structure of the flesh segment primordia or primary flesh segment. In the first stage of flesh segment development, the ovary structure of the pistil expanded.</p>
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<p>Fluctuations of the endogenous hormone levels in the flower buds of ‘Biqi’ and ‘Zaojia’ during flesh segment development. Samples for Stage I were collected before the initiation of flesh segment development, on 26 December for ‘Biqi’ and 17 November for ‘Zaojia’. Samples for Stage II were collected at the stage of flesh segment primordial cell formation, on 18 February for ‘Biqi’ and 26 December for ‘Zaojia’. Samples for Stage III were collected after the formation of the primary flesh segment, on 9 March for ‘Biqi’ and 18 February for ‘Zaojia’. Significant differences were analyzed using Duncan’s test, with different lowercase letters indicating significant differences (<span class="html-italic">p</span> &lt; 0.05). In both ‘Biqi’ and ‘Zaojia’, the levels of JA, JA-Ile, and IAA were significantly downregulated at the initiation of flesh segment development. In ‘Zaojia’, the level of JA decreased substantially (by approximately 93.83%) after primordium formation. Meanwhile, in ‘Biqi’, the level of iP increased significantly during the subsequent stages of flesh segment development. In contrast, ‘Zaojia’ exhibited an initial decrease in the iP level, followed by a recovery. However, the iP level in ‘Zaojia’ remained lower than that in ‘Biqi’.</p>
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<p>Correlations between flesh segment development stages and endogenous hormone levels. Pearson’s correlation analysis was conducted, and the numerical values in the figure represented correlation coefficients (<span class="html-italic">r</span> values). Positive or negative values indicate the direction of the correlations, while asterisks denote the significance of the correlations (* <span class="html-italic">p</span> &lt; 0.05 and ** <span class="html-italic">p</span> &lt; 0.01). In both (<b>a</b>) ‘Biqi’ and (<b>b</b>) ‘Zaojia’, the degree of flesh segment development was negatively correlated with the levels of IAA, JA, and JA-Ile. Moreover, there were significant correlations among the levels of these three hormones throughout the flesh segment development process.</p>
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<p>Statistics on the number of DEGs before and after flesh segment development. (<b>a</b>) A Venn diagram on the number of DEGs. (<b>b</b>) A bar chart on the number of upregulated and downregulated DEGs.</p>
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<p>WGCNA analysis of genes identified in the transcriptome data on bayberry flesh segment development. (<b>a</b>) Statistics on the number of genes in each module. (<b>b</b>) A heatmap showing the associations between WGCNA co-expression modules and traits related to bayberry flesh segment development. The MEcyan, MEblue, and MEmidnightblue modules were significantly correlated with the development of bayberry flesh segments.</p>
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<p>A heatmap of the core gene expression levels in the biological pathways enriched in (<b>a</b>) ‘Biqi’ and (<b>b</b>) ‘Zaojia’. The identified core genes included genes involved in plant hormone signaling pathways, such as <span class="html-italic">LAX3</span> and <span class="html-italic">IAA7</span>, as well as genes involved in pistil development, including <span class="html-italic">CRC</span>.</p>
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<p>KEGG pathway enrichment analysis of DEGs involved during flesh segment development in (<b>a</b>) ‘Biqi’ and (<b>b</b>) ‘Zaojia’. These plant hormone signaling pathways were highly enriched during the flesh segment development process.</p>
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<p>Heatmap analysis of the DEGs upregulated in the plant hormone signaling pathways involved during flesh segment development in ‘Biqi’.</p>
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<p>Heatmap analysis of DEGs that were upregulated in the plant hormone signaling pathways involved during flesh segment development in ‘Zaojia’.</p>
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<p>Relative expression levels of genes related to flesh segment development in ‘Biqi’.</p>
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<p>Relative expression levels of genes related to flesh segment development in ‘Zaojia’.</p>
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<p>Immunofluorescence localization of auxin in the (<b>a</b>) longitudinal and (<b>b</b>) transverse sections of flesh segments. The bayberry variety used for the immunofluorescence localization analysis was ‘Biqi’, and all scale bars were set to 200 μm. In (<b>a</b>), the dashed line indicates a longitudinal section of a single flesh segment, where the auxin fluorescence signals are enriched at the top and side walls of the flesh segment, forming a continuous linear distribution along the contour marked by the dashed line. In (<b>b</b>), the arrow indicates the central vascular bundle within the cross-section of the flesh segment, where the enrichment of auxin fluorescence signals is observed.</p>
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<p>The mechanism of morphological development of flesh segment. (<b>a</b>) Distribution pattern of IAA in flesh segment. The red dots represent IAA, the black dashed box indicates the enriched area of IAA, and the red dashed arrow represents the possible transport direction of IAA. (<b>b</b>) Potential molecular mechanisms underlying the morphological development of flesh segment. IAA is enriched in the outer cell layer of the flesh segment, which may be the reason for the unique morphology of the flesh segment. In addition, IAA is also enriched in the central vascular system, where there may be transport of IAA. It is speculated that there are two possible transport directions: one is that IAA is transported through the vascular system to the top of the flesh segment and then distributed to the outer edge cell layer, and the other is that IAA is transported horizontally within the vascular system and directly distributed to the outer edge cell layer. Molecular pathways for predicting the development of flesh segment morphology based on transcriptome analysis of genes selected through functional analysis and hormone pathway analysis. According to the results, it was found that there is a strong correlation between auxin and jasmonic acid during the development of the flesh segment. It is speculated that the development of the flesh segment may involve the joint regulation of two hormone pathways, and the regulatory network may involve <span class="html-italic">LAX</span>\<span class="html-italic">CRC</span>\<span class="html-italic">SEP</span> as the upstream, <span class="html-italic">IAA7</span> as the downstream auxin regulatory pathway, and <span class="html-italic">JAZ6</span> as the responsive jasmonic acid regulatory pathway.</p>
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17 pages, 2934 KiB  
Article
An Improved Small Target Segmentation Model Based on Mask Dino
by Jun Yang, Xu Chen, Yun Guan, Yixuan Hu and Gang Ge
Appl. Sci. 2025, 15(4), 1832; https://doi.org/10.3390/app15041832 - 11 Feb 2025
Viewed by 398
Abstract
To address the issue of low segmentation accuracy for small objects in the Mask Dino segmentation method, we propose an improved small object segmentation model called FFMask Dino. Initially, we introduce scaled cosine attention and the log-cpb method into the Swin Transformer backbone [...] Read more.
To address the issue of low segmentation accuracy for small objects in the Mask Dino segmentation method, we propose an improved small object segmentation model called FFMask Dino. Initially, we introduce scaled cosine attention and the log-cpb method into the Swin Transformer backbone network. Subsequently, by adjusting the network structure, we enhance the feature extraction process, which helps the model maintain generalization across different datasets and reduces the risk of overfitting. Lastly, we propose the FFPN module to optimize the pathways for feature fusion and transmission. The improved FPN reduces unnecessary computations, accelerates model inference speed, and integrates multi-scale feature details and high-level semantic information to complement object features, thereby enhancing model segmentation accuracy. Experimental results demonstrate that the improved segmentation model achieves a mean Intersection over Union (mIoU) of 42.15% on the ADE20K dataset for semantic segmentation tasks, representing a 0.96% increase compared to the Mask Dino method. On the CoCo dataset for instance segmentation tasks, with the Swin Transformer backbone, the Mask AP and Box AP are 47.10 and 52.60, respectively, showing improvements of 1% and 1.3% over the Mask Dino method. With the ResNet-50 backbone, the Mask AP and Box AP are 40.00 and 44.10, respectively, with improvements of 0.5% and 0.9% over the Mask Dino method. For the CoCo dataset’s panoptic segmentation tasks, with the Swin Transformer backbone, the PQ is 54.95, showing a 0.4% increase over the Mask Dino method. With the ResNet-50 backbone, the PQ is 46.93, showing a 0.9% increase over the Mask Dino method. These results effectively demonstrate the improved accuracy and precision of Mask Dino in segmenting small objects across various segmentation tasks. Full article
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<p>The structure diagram of the improved Mask Dino.</p>
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<p>Improved backbone network Transformer structure diagram.</p>
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<p>FFPN decomposition diagram.</p>
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<p>(<b>a</b>) Original images from the ADE20K dataset; (<b>b</b>) visualization of the label map; (<b>c</b>) visualization of the segmentation results from Mask Dino; (<b>d</b>) visualization of the segmentation results from FFMask Dino in this paper.</p>
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<p>(<b>a</b>) Original Image; (<b>b</b>) visualization of the label map; (<b>c</b>) visualization of the segmentation results from Mask Dino; (<b>d</b>) Visualization of the segmentation results with the Transformer * module; (<b>e</b>) visualization of the segmentation results with the FFPN module; (<b>f</b>) visualization of the segmentation results from the FFMask Dino model in this paper.</p>
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14 pages, 4382 KiB  
Article
One-Step Fabrication of Poly(vinylidene Fluoride-Co-Hexafluoropropylene)/Perfluorodecyltriethoxysilane Fibrous Membranes with Waterproof, Breathable, and Radiative Cooling Properties
by Aohan Hou, Juan Xie, Xiaohui Wu, Guichun Lin, Yayi Yuan, Xi Liu, Yancheng Wu, Feng Gan, Yangling Li, Yuxiao Wu, Gang Huang, Zhengrong Li and Jing Zhao
Molecules 2025, 30(4), 763; https://doi.org/10.3390/molecules30040763 - 7 Feb 2025
Viewed by 382
Abstract
Functional membranes with waterproof, breathable, and thermal regulation capabilities are increasingly sought after across various industries. However, developing such functional membranes commonly involves complex multi-step preparation processes. Herein, we introduced perfluorodecyltriethoxysilane (FAS) into the poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) solution for one-step electrospinning, successfully fabricating [...] Read more.
Functional membranes with waterproof, breathable, and thermal regulation capabilities are increasingly sought after across various industries. However, developing such functional membranes commonly involves complex multi-step preparation processes. Herein, we introduced perfluorodecyltriethoxysilane (FAS) into the poly(vinylidene fluoride-co-hexafluoropropylene) (PVDF-HFP) solution for one-step electrospinning, successfully fabricating membranes that combine these properties. The hydrophobicity of the PVDF-HFP/FAS membrane was greatly improved with the water contact angle increased from 120.6° to 142.9° and the solar reflectance rising from 72% to 92% due to the presence of fluorocarbon segments. The synergistic effect of enhanced hydrophobicity, small pore size, and elevated solar reflectivity resulted in robust water resistance (62 kPa), excellent water vapor transmission rate (12.4 kg m−2 d−1), and superior cooling performance (6.4 °C lower than commercial cotton fabrics). These findings suggest that the proposed PVDF-HFP/FAS membranes, characterized by desired multifunction characteristics and scalable production, hold great potential for application in diverse strategic fields. Full article
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<p>(<b>a</b>) Schematic diagram of PVDF-HFP/FAS multifunctional membrane. (<b>b</b>) Schematic exhibiting waterproofness, breathability, and radiative cooling of the designed materials. (<b>c</b>) Cross-sectional image of the PVDF-HFP/FAS membrane. (<b>d</b>) IR pictures of PVDF-HFP/FAS membrane and cotton after 10 min of exposure to sunlight. (<b>e</b>) Optical image of the large-sized membrane.</p>
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<p>(<b>a</b>–<b>d</b>) SEM images of different pure PVDF-HFP fibrous membranes. (<b>e</b>–<b>h</b>) The diameter distributions of corresponding PVDF-HFP fibrous membranes. The number “x” in the PH-x sample refers to the weight percent concentration of PVDF-HFP polymer.</p>
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<p>(<b>a</b>) Thickness, (<b>b</b>) pore size distribution, (<b>c</b>) d<sub>max</sub> and porosity, and (<b>d</b>) Ra of different pure PVDF-HFP fibrous membranes.</p>
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<p>(<b>a</b>) WCA, (<b>b</b>) water resistance and water vapor permeability, (<b>c</b>) air permeability, and (<b>d</b>) stress–strain curves of different pure PVDF-HFP fibrous membranes.</p>
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<p>(<b>a</b>) Reflectance and (<b>b</b>) infrared emissivity of different pure PVDF-HFP fibrous membranes.</p>
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<p>(<b>a–d</b>) SEM images of different PVDF-HFP/FAS composite fibrous membranes. The number “y” in the PF-18-y sample refers to the weight percent concentration of FAS.</p>
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<p>(<b>a</b>) Thickness, (<b>b</b>) pore size distribution, (<b>c</b>) d<sub>max</sub> and porosity, and (<b>d</b>) Ra of different PVDF-HFP/FAS composite fibrous membranes.</p>
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<p>(<b>a</b>) XPS, (<b>b</b>) Si and F atomic percentage, (<b>c</b>) FTIR, and (<b>d</b>) WCA of different PVDF-HFP/FAS composite fibrous membranes.</p>
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<p>(<b>a</b>) Hydrostatic pressure and WVT rate of different PVDF-HFP/FAS composite fibrous membranes. (<b>b</b>) Image exhibiting waterproof and breathable performance. (<b>c</b>) Air permeability and (<b>d</b>) tensile strength and elongation of different PVDF-HFP/FAS composite fibrous membranes.</p>
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<p>(<b>a</b>) Reflectance of different PVDF-HFP/FAS composite fibrous membranes. (<b>b</b>) Emissivity of the PF-18-7 sample.</p>
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<p>(<b>a</b>,<b>b</b>) Photograph and schematic of the apparatus used to test the outdoor cooling effect. (<b>c</b>) The recorded solar radiation. (<b>d</b>) The real-time temperatures of ambient air, uncovered, cotton-covered, and PF-18-7 sample-covered skin simulator. Temperature data recorded on a clear day in Jiangmen, China (27 September 2024 10:30 to 15:30). (<b>e</b>) The digital and infrared images of the models without cover, covered with cotton, and covered with PF-18-7 sample.</p>
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26 pages, 13415 KiB  
Article
A Methodology for the Multitemporal Analysis of Land Cover Changes and Urban Expansion Using Synthetic Aperture Radar (SAR) Imagery: A Case Study of the Aburrá Valley in Colombia
by Ahmed Alejandro Cardona-Mesa, Rubén Darío Vásquez-Salazar, Juan Camilo Parra, César Olmos-Severiche, Carlos M. Travieso-González and Luis Gómez
Remote Sens. 2025, 17(3), 554; https://doi.org/10.3390/rs17030554 - 6 Feb 2025
Viewed by 1335
Abstract
The Aburrá Valley, located in the northwestern region of Colombia, has undergone significant land cover changes and urban expansion in recent decades, driven by rapid population growth and infrastructure development. This region, known for its steep topography and dense urbanization, faces considerable environmental [...] Read more.
The Aburrá Valley, located in the northwestern region of Colombia, has undergone significant land cover changes and urban expansion in recent decades, driven by rapid population growth and infrastructure development. This region, known for its steep topography and dense urbanization, faces considerable environmental challenges. Monitoring these transformations is essential for informed territorial planning and sustainable development. This study leverages Synthetic Aperture Radar (SAR) imagery from the Sentinel-1 mission, covering 2017–2024, to propose a methodology for the multitemporal analysis of land cover dynamics and urban expansion in the valley. The novel proposed methodology comprises several steps: first, monthly SAR images were acquired for every year under study from 2017 to 2024, ensuring the capture of surface changes. These images were properly calibrated, rescaled, and co-registered. Then, various multitemporal fusions using statistics operations were proposed to detect and find different phenomena related to land cover and urban expansion. The methodology also involved statistical fusion techniques—median, mean, and standard deviation—to capture urbanization dynamics. The kurtosis calculations highlighted areas where infrequent but significant changes occurred, such as large-scale construction projects or sudden shifts in land use, providing a statistical measure of surface variability throughout the study period. An advanced clustering technique segmented images into distinctive classes, utilizing fuzzy logic and a kernel-based method, enhancing the analysis of changes. Additionally, Pearson correlation coefficients were calculated to explore the relationships between identified land cover change classes and their spatial distribution across nine distinct geographic zones in the Aburrá Valley. The results highlight a marked increase in urbanization, particularly along the valley’s periphery, where previously vegetated areas have been replaced by built environments. Additionally, the visual inspection analysis revealed areas of high variability near river courses and industrial zones, indicating ongoing infrastructure and construction projects. These findings emphasize the rapid and often unplanned nature of urban growth in the region, posing challenges to both natural resource management and environmental conservation efforts. The study underscores the need for the continuous monitoring of land cover changes using advanced remote sensing techniques like SAR, which can overcome the limitations posed by cloud cover and rugged terrain. The conclusions drawn suggest that SAR-based multitemporal analysis is a robust tool for detecting and understanding urbanization’s spatial and temporal dynamics in regions like the Aburrá Valley, providing vital data for policymakers and planners to promote sustainable urban development and mitigate environmental degradation. Full article
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<p>The Aburrá Valley (white line) between the valleys of the Magdalena and Cauca rivers. Data were acquired from ALOS PALSAR Terrain Corrected and data from IGAC.</p>
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<p>Region of interest (yellow bounding box) selected from the interior of the Aburrá Valley (red line) and the municipalities that are part of it (green lines). Data were acquired from IGAC.</p>
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<p>Proposed methodology for the multitemporal analysis <math display="inline"><semantics> <mrow> <mi>S</mi> <mi>M</mi> <msub> <mi>A</mi> <mn>1</mn> </msub> </mrow> </semantics></math>.</p>
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<p>Proposed methodology for kurtosis multitemporal analysis, <math display="inline"><semantics> <mrow> <mi>S</mi> <mi>M</mi> <msub> <mi>A</mi> <mn>2</mn> </msub> </mrow> </semantics></math>.</p>
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<p>Proposed methodology for analysis of zonal land cover changes.</p>
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<p>Samples of resulting images of the multitemporal analysis methodology proposed in <math display="inline"><semantics> <mrow> <mi>S</mi> <mi>M</mi> <msub> <mi>A</mi> <mn>1</mn> </msub> </mrow> </semantics></math> (<b>a</b>) <math display="inline"><semantics> <mrow> <mi>M</mi> <msub> <mi>F</mi> <mrow> <mi>M</mi> <mi>d</mi> <mi>n</mi> </mrow> </msub> </mrow> </semantics></math>, (<b>b</b>) <math display="inline"><semantics> <mrow> <mi>M</mi> <msub> <mi>F</mi> <mi>σ</mi> </msub> </mrow> </semantics></math>, (<b>c</b>) <math display="inline"><semantics> <mrow> <mi>M</mi> <msub> <mi>F</mi> <mi>M</mi> </msub> </mrow> </semantics></math>, (<b>d</b>) <math display="inline"><semantics> <mrow> <mi>M</mi> <msub> <mi>F</mi> <mrow> <mi>C</mi> <mi>V</mi> </mrow> </msub> </mrow> </semantics></math>, (<b>e</b>) <math display="inline"><semantics> <mrow> <mi>M</mi> <msub> <mi>F</mi> <mi>C</mi> </msub> </mrow> </semantics></math> for the year 2018, and (<b>f</b>) <math display="inline"><semantics> <mrow> <mi>M</mi> <msub> <mi>F</mi> <mi>K</mi> </msub> </mrow> </semantics></math> of <math display="inline"><semantics> <mrow> <mi>S</mi> <mi>M</mi> <msub> <mi>A</mi> <mn>2</mn> </msub> </mrow> </semantics></math> for 2017–2014. Scale, coordinate frame (grid), and north correspond to the region described in the Study area section.</p>
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<p>Areas of analysis of the results by the SMA1 methodological route and kurtosis. (<b>A</b>). Central Park in Bello (<b>B</b>). Parques del Río Medellín (<b>C</b>). Arkadia Shopping center; (<b>D</b>). Peldar Plant (<b>E</b>). La García water supply reservoir (<b>F</b>). Conasfaltos dam (<b>G</b>). La Ayurá stream basin in Envigado (<b>H</b>). Central Park in Bello (<b>I</b>). Avenida Regional Norte (<b>J</b>). Vía Distribuidora Sur.</p>
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<p>Side-by-side comparison of the Aburrá Valley. (<b>a</b>) Division into 9 geographical zones. (<b>b</b>) The corresponding correlation coefficients for 5 different land cover change types of the 9 zones.</p>
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<p>Color maps for every change class in the Aburrá Valley’s nine geographical zones.</p>
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25 pages, 1523 KiB  
Article
Attitudes, Time Pressure, and Behavior Change Techniques Affect Route Journey Planning Decisions: Evidence from an RCT
by Emma Maier, Lewis Turner-Brown, Andrew Broadbent and Jonathan Freeman
Sustainability 2025, 17(3), 1297; https://doi.org/10.3390/su17031297 - 5 Feb 2025
Viewed by 631
Abstract
Transport emissions are a major contributor to global CO2 emissions, requiring interventions to promote sustainable travel behaviors. This study examines how behavior change techniques (BCTs), attitudinal and behavioral segmentation, and time pressure influence green route selection in a simulated journey-planning app. Using [...] Read more.
Transport emissions are a major contributor to global CO2 emissions, requiring interventions to promote sustainable travel behaviors. This study examines how behavior change techniques (BCTs), attitudinal and behavioral segmentation, and time pressure influence green route selection in a simulated journey-planning app. Using a randomized 2 × 3 × 3 factorial design, 600 UK participants completed travel booking tasks under three time-pressure scenarios (low, moderate, high) using either a control app or a BCT-enhanced intervention app. Participants were segmented based on environmental attitudes, public transport preferences, and travel needs. Multilevel logistic regression showed significant main effects for condition, segment, and time pressure. Participants using the intervention app were more likely to select green routes (5.39, p < 0.001). Segments with a more positive attitude to public transport demonstrated higher baseline green route selection compared to those with low public transport attitudes (odds ratio [OR] = 0.31, p = 0.020). Moderate time pressure facilitated the highest likelihood of green route selection, while low (OR = 0.16, p < 0.001) and high (OR = 0.48, p < 0.001) time pressures reduced green bookings. Interaction effects were non-significant, potentially reflecting the sample size. The findings highlight the potential of BCT-enhanced apps to promote sustainable travel, particularly when tailored to user segments and designed to address time pressure. Future research should explore real-world applications and intervention durability. Full article
(This article belongs to the Section Psychology of Sustainability and Sustainable Development)
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<p>Screen images from control condition app (no BCTs): (<b>a</b>) phone home screen with app button showing app logo; (<b>b</b>) pop-up showing basic account information (limited feedback on behaviors/outcomes); (<b>c</b>) search results page; (<b>d</b>) route detail page; (<b>e</b>) booking confirmation.</p>
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<p>Screen images from intervention condition app (including BCTs): (<b>a</b>) phone home screen with “widget” button emphasizing points from past engagement; (<b>b</b>) notification showing congratulatory messaging and prompting self-praise; (<b>c</b>) goal-setting/review pop-up; (<b>d</b>) pop-up/profile page providing feedback on behavior and outcomes; (<b>e</b>) results page showing goal- and outcome-relevant icons; (<b>f</b>) route detail page providing information to remove uncertainty (e.g., bike dock availability, walking route incline, etc.); (<b>g</b>) prompt to review choice in line with goals and behaviors of relevant others; (<b>h</b>) option to reduce route options to conserve mental resources; (<b>i</b>) congratulatory messaging and reward (badge); (<b>j</b>) booking confirmation reinforcing choice and awarding a point.</p>
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<p>Proportion of participants making green route selection (%) by attitudinal segment, time pressure level, and condition.</p>
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30 pages, 9951 KiB  
Article
Characterizing the Full Climate Impact of Individual Real-World Flights Using a Linear Temperature Response Model
by Mohamed Awde and Charles Stuart
Aerospace 2025, 12(2), 121; https://doi.org/10.3390/aerospace12020121 - 5 Feb 2025
Viewed by 528
Abstract
Aviation’s non-CO2 effects account for approximately 66% of the sector’s Effective Radiative Forcing (ERF). However, non-CO2 emissions and their climate effects are particularly challenging to assess due to the number of variables involved. This research provides a framework for characterizing the [...] Read more.
Aviation’s non-CO2 effects account for approximately 66% of the sector’s Effective Radiative Forcing (ERF). However, non-CO2 emissions and their climate effects are particularly challenging to assess due to the number of variables involved. This research provides a framework for characterizing the full climate impact of individual real-world flights in terms of global surface temperature change (ΔT) with the aid of a validated CFM56-7B26/3 engine model and spatially and temporally resolved meteorological data. Different modelling methods were used to evaluate NOx and soot emissions and the relative differences between them were quantified, while a contrail formation model was implemented to quantify the distances travelled where persistent contrails were formed. The ΔT was evaluated over 77 years using a Linear Temperature Response Model (LTR). The results show that NOx-induced effects such as the increase in short-term ozone had the highest impact on ΔT in the first year of emissions, while CO2 was more detrimental to ΔT in the long term. Unlike the mid and long-range flights examined, the climb segment of the short-range flight had a more significant impact on ΔT than the cruise segment. ΔT sensitivity studies for different emission modelling methods showed differences up to 13% for NOx and 14% for soot. Full article
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<p>Overview of the aircraft engine emissions and their resultant effects and climate impacts adapted from [<a href="#B11-aerospace-12-00121" class="html-bibr">11</a>].</p>
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<p>Overview of methodology implemented in this study.</p>
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<p>Generalized block diagram of CFM56-7B26/3 NPSS model utilized on the B737-800NG.</p>
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<p>Validation of the calibrated SUAVE-NPSS B737-800NG model against actual fuel burn data for a 500 NM mission [<a href="#B33-aerospace-12-00121" class="html-bibr">33</a>].</p>
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<p>Generalized summary of NO<sub>x</sub> and soot emission models implemented in this study.</p>
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<p>Saturation vapour pressure curves with respect to water and ice showing contrail formation areas according to the location of the exhaust plume mixing line [<a href="#B43-aerospace-12-00121" class="html-bibr">43</a>].</p>
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<p>Overview of contrail formation conditions.</p>
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<p>Forcing factor for contrails and NO<sub>x</sub>-induced forcing agents at different altitudes [<a href="#B23-aerospace-12-00121" class="html-bibr">23</a>].</p>
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<p>General overview of the methodology applied using the LTR model in this study to evaluate <span class="html-italic">ΔT</span>.</p>
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<p>Modelled <span class="html-italic">EINO<sub>x</sub></span> along the flight paths using the three correlation methods for (<b>a</b>) flight 1 (<b>b</b>) flight 2 and (<b>c</b>) flight 3 (dashed lines are used to separate the different flight segments).</p>
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<p>Relative errors between the different methods used for modelling <span class="html-italic">EINO<sub>x</sub></span> for all three flights: (<b>a</b>) P3T3 vs. BFFM2 (<b>b</b>) P3T3 vs. DLR (<b>c</b>) BFFM2 vs. DLR (blue data points represent the <span class="html-italic">EINO<sub>x</sub></span> values, solid line represents the best-fit of the data points, and dashed lines represent the deviations from the best-fit line).</p>
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<p>Relative errors between the different methods used for modelling <span class="html-italic">EINO<sub>x</sub></span> for all three flights: (<b>a</b>) P3T3 vs. BFFM2 (<b>b</b>) P3T3 vs. DLR (<b>c</b>) BFFM2 vs. DLR (blue data points represent the <span class="html-italic">EINO<sub>x</sub></span> values, solid line represents the best-fit of the data points, and dashed lines represent the deviations from the best-fit line).</p>
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<p>Modelled <span class="html-italic">EIsoot</span> along the flight paths using the two correlation methods for (<b>a</b>) flight 1, (<b>b</b>) flight 2, and (<b>c</b>) flight 3 (dashed lines are used to separate the different flight segments).</p>
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<p>Modelled <span class="html-italic">EIsoot</span> along the flight paths using the two correlation methods for (<b>a</b>) flight 1, (<b>b</b>) flight 2, and (<b>c</b>) flight 3 (dashed lines are used to separate the different flight segments).</p>
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<p>Fuel-proportional emissions for the three flights on a per-flight segment basis (<b>a</b>) CO<sub>2</sub> (<b>b</b>) H<sub>2</sub>O (<b>c</b>) SO<sub>4</sub>.</p>
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<p>Non-fuel-proportional emissions for the three flights on a per-flight segment basis using the different correlation methods (<b>a</b>) NO<sub>x</sub> (<b>b</b>) soot.</p>
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<p>Formation of (<b>a</b>) contrails and (<b>b</b>) persistent contrails along a schematic path of flight 2.</p>
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<p>Formation of (<b>a</b>) contrails and (<b>b</b>) persistent contrails along a schematic path of flight 3.</p>
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<p>Global surface temperature change caused by (<b>a</b>) flight 1, (<b>b</b>) flight 2, and (<b>c</b>) flight 3.</p>
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<p>Global surface temperature change caused by (<b>a</b>) flight 1, (<b>b</b>) flight 2, and (<b>c</b>) flight 3.</p>
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<p>Global surface temperature change sensitivity to NO<sub>x</sub> modelling method (<b>a</b>) flight 1, (<b>b</b>) flight 2, and (<b>c</b>) flight 3.</p>
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<p>Global surface temperature change sensitivity to NO<sub>x</sub> modelling method (<b>a</b>) flight 1, (<b>b</b>) flight 2, and (<b>c</b>) flight 3.</p>
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<p>Global surface temperature change sensitivity to soot modelling method (<b>a</b>) flight 1, (<b>b</b>) flight 2, and (<b>c</b>) flight 3.</p>
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<p>Global surface temperature change sensitivity to soot modelling method (<b>a</b>) flight 1, (<b>b</b>) flight 2, and (<b>c</b>) flight 3.</p>
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<p>Global surface temperature change sensitivity to flight segment (<b>a</b>) flight 1, (<b>b</b>) flight 2, and (<b>c</b>) flight 3.</p>
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<p>Global surface temperature change sensitivity to flight segment (<b>a</b>) flight 1, (<b>b</b>) flight 2, and (<b>c</b>) flight 3.</p>
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25 pages, 7090 KiB  
Article
Combined Bulked Segregant Analysis-Sequencing and Transcriptome Analysis to Identify Candidate Genes Associated with Cold Stress in Brassica napus L
by Jiayi Jiang, Rihui Li, Kaixuan Wang, Yifeng Xu, Hejun Lu and Dongqing Zhang
Int. J. Mol. Sci. 2025, 26(3), 1148; https://doi.org/10.3390/ijms26031148 - 28 Jan 2025
Viewed by 624
Abstract
Cold tolerance in rapeseed is closely related to its growth, yield, and geographical distribution. However, the mechanisms underlying cold resistance in rapeseed remain unclear. This study aimed to explore cold resistance genes and provide new insights into the molecular mechanisms of cold resistance [...] Read more.
Cold tolerance in rapeseed is closely related to its growth, yield, and geographical distribution. However, the mechanisms underlying cold resistance in rapeseed remain unclear. This study aimed to explore cold resistance genes and provide new insights into the molecular mechanisms of cold resistance in rapeseed. Rapeseed M98 (cold-sensitive line) and D1 (cold-tolerant line) were used as parental lines. In their F2 population, 30 seedlings with the lowest cold damage levels and 30 with the highest cold damage levels were selected to construct cold-tolerant and cold-sensitive pools, respectively. The two pools and parental lines were analyzed using bulk segregant sequencing (BSA-seq). The G’-value analysis indicated a single peak on Chromosome C09 as the candidate interval, which had a 2.59 Mb segment with 69 candidate genes. Combined time-course and weighted gene co-expression network analyses were performed at seven time points to reveal the genetic basis of the two-parent response to low temperatures. Twelve differentially expressed genes primarily involved in plant cold resistance were identified. Combined BSA-seq and transcriptome analysis revealed BnaC09G0354200ZS, BnaC09G0353200ZS, and BnaC09G0356600ZS as the candidate genes. Quantitative real-time PCR validation of the candidate genes was consistent with RNA-seq. This study facilitates the exploration of cold tolerance mechanisms in rapeseed. Full article
(This article belongs to the Special Issue Molecular Genetics and Plant Breeding, 5th Edition)
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Figure 1

Figure 1
<p>Phenotypic observation of rapeseed seedlings in response to cold stress. (<b>a</b>) Rapeseed <span class="html-italic">D1</span> and <span class="html-italic">M98</span> seedlings grown under normal conditions (22 °C) in the plant chamber. (<b>b</b>) Phenotypes of <span class="html-italic">D1</span> and <span class="html-italic">M98</span> seedlings after cold treatment (−4 °C, 24 h) in the plant chamber. (<b>c</b>) <span class="html-italic">D1</span> and <span class="html-italic">M98</span> seedlings at 24 days of recovery (22 °C) in the plant chamber. The survival rates were evaluated at this stage, as shown in the images. (<b>d</b>–<b>h</b>) The phenotype of seedlings with cold damage level 0–4 in the field, respectively. Bar, 5 cm (<b>a</b>–<b>h</b>).</p>
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<p>Manhattan plots showing the distribution of G’-Value on the chromosomes. (<b>a</b>) Manhattan plots showing the distribution of G’-Value on all 19 chromosomes. The blue box is a single pink on the chromosome C09. (<b>b</b>) Enlarged view of chromosome C09 in (<b>a</b>), highlighting the single peak on chromosome C09. The blue box is the candidate interval for this study.</p>
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<p>Variation types of candidate genes in the candidate interval (CI).</p>
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<p>Statistics of the expressed genes. (<b>a</b>) Statistics of the expressed genes in all samples. (<b>b</b>) Statistics of the DEGs in <span class="html-italic">D1</span>, <span class="html-italic">M98,</span> and D1 vs. M98. (<b>c</b>) Statistics of the DEGs between two adjacent low-temperature treatment time points in <span class="html-italic">D1</span> and <span class="html-italic">M98</span>. Line charts showing the number of the expressed genes during different sampling time points (1st–7th are described in the methods) in rapeseed <span class="html-italic">D1</span> and <span class="html-italic">M98</span>. CK: control; T, low-temperature treatment; D1 vs. M98, a gene set after removing D1_CK from the D1_T vs. a gene set after removing M98_CK from the M98_T.</p>
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<p>Enrichment analyses of the GO annotation and KEGG pathway. The GO annotation analysis of gene set in the 6th vs. 5th time points in <span class="html-italic">D1</span> (<b>a</b>) and <span class="html-italic">M98</span> (<b>c</b>). The GO annotation analysis of the intersection of the 5th and 6th time points in <span class="html-italic">D1</span> (<b>b</b>). The KEGG pathway analysis of gene set in the 6th vs. 5th time points in <span class="html-italic">D1</span> (<b>d</b>). The KEGG pathway analysis of the intersection of the 5th and 6th time points in <span class="html-italic">D1</span> (<b>e</b>) and <span class="html-italic">M98</span> (<b>f</b>).</p>
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<p>Different gene expression patterns based on the time-course analysis. Each cluster represents a trend of gene expression, and the numbers at the bottom indicate the number of genes in the cluster. Different colored curves represent cultivars under low-temperature treatment conditions, and each curve represents the median profile of genes at different low-temperature treatment time points. CK, control; T, treatment; Sampling time points 1st–7th are described in the methods.</p>
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<p>GO enrichment analysis of the predominant expression in <span class="html-italic">D1</span> and <span class="html-italic">M98</span>. GO enrichment analysis of the predominant expression in <span class="html-italic">D1</span> (<b>a</b>) and <span class="html-italic">M98</span> (<b>b</b>), including biological process, cellular component, and molecular function. The different colors represent different clusters. The X-axis represents −log<sub>10</sub> (<span class="html-italic">p</span>-value), and the enriched GO terms are indicated on the Y-axis.</p>
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<p>Co-expression network construction and overlapping analysis with MaSigPro. (<b>a</b>,<b>b</b>) Weighted gene co-expression network analysis of the genes with dominant expression in <span class="html-italic">D1</span> and <span class="html-italic">M98</span> at seven time points of low-temperature treatment. Each row represents a module, and the correlation coefficient and the <span class="html-italic">p</span>-value calculated using Fisher’s exact test are shown in each square. The table is color-coded by correlation according to the color legend. The intensity and direction of correlations are indicated on the right-hand side of the heat map (red, positive; blue, negative). (<b>c</b>,<b>d</b>) Overlapping analyses of genes in the five clusters and eight modules in <span class="html-italic">D1</span> and the four clusters and nine modules in <span class="html-italic">M98</span>. Sampling time points 1st–7th are described in the methods.</p>
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