Search Results (87)

Grape seeds are a rich source of bioactive compounds, especially polyphenols, which are known for their antioxidant and anti-aging properties. The aim of this study was to extract phytochemicals from the Lebanese grape variety “Obeidi” and incorporate them into liposomes and hyalurosomes in order to select the most effective formulation for improving skin properties and protecting against oxidative damage. Grape seeds were extracted by a sustainable ethanol-water method, yielding a total phenolic content (TPC) of 376 ± 16 mg/g (gallic acid equivalents) and an antioxidant activity of 0.58 ± 0.04 µg/mL (trolox equivalents). HPLC analysis identified catechins (4.51 mg/g), gallic acid (3.2 mg/g) and epicatechins (0.88 mg/g) as the dominant phenolics. The extract was encapsulated in liposomes and hyalurosomes with a mean diameter of 148–159 nm, polydispersity index < 0.3, and zeta potentials of −47 to −44 mV, and remained stable for three months at 4 °C. In vitro studies showed that hyalurosomes significantly increased keratinocyte viability by up to 144% and provided better protection against oxidative stress compared to liposomes. A 12-week clinical study involving 40 volunteers was conducted to evaluate the efficacy of extract-loaded hyalurosomes as an “anti-aging serum” by assessing various skin parameters. This treatment resulted in improved skin hydration, a reduction in wrinkle depth, and a slight decrease in melanin and erythema levels as demonstrated by imaging. These results are promising for cosmeceutical applications using grape seed extract loaded in hyalurosomes. Full article

In this article, a new extension of the standard Laplace distribution is introduced for house price modeling. Certain important properties of the new distribution are deducted throughout this study. We used the new extension of the Laplace model to conduct a thorough economic risk assessment utilizing several metrics, including the value-at-risk (VaR), the peaks over a random threshold value-at-risk (PORT-VaR), the tail value-at-risk (TVaR), the mean of order-P (MO^P), and the peaks over a random threshold based on the mean of order-P (PORT-MO^P). These metrics capture different facets of the tail behavior, which is essential for comprehending the extreme median values in the Boston house price data. Notably, PORT-VaR improves the risk evaluations by incorporating randomness into the selection of the thresholds, whereas VaR and TVaR focus on measuring the potential losses at specific confidence levels, with TVaR offering insights into significant tail risks. The MO^P method aids in balancing the reliability goals while optimizing the performance in the face of uncertainty. Full article

The aim of this study was to evaluate a natural coagulant, Moringa oleifera seeds (MOC), to reduce the color concentration in treated tequila vinasses (TVs). TV-A was the effluent of horizontal subsurface flow wetlands (HSSFW); TV-B was the effluent of vertical up-flow wetlands (VUFW); and TV-C was the effluent of vertical down-flow constructed wetlands (VDFW). Raw TVs were also evaluated with MOC. Jar tests were performed to find the optimal dose and pH value for apparent color (AC) removal. With the optimal dose and pH for each type of TV, tests were performed in triplicate to evaluate the removal of apparent color (AC), true color (TC), turbidity, total suspended solids (TSS), chemical oxygen demand (COD), and electrical conductivity (EC). For TV-A and TV-B, the optimal values were 1 g/L of MOC and pH 8, and the removals were 52%, 43%, 50% and 72% of AC, turbidity, TC, and TSS, respectively. For TV-C, the optimal values were 2.5 g/L and pH 5, with removals of 66%, 73%, and 98% for AC, TC, and TSS, respectively. For TV-D, the MOC had no coagulant effect in any of the experimental conditions evaluated, probably due to the high concentration of turbidity and TSS in the raw vinasses, which prevented the interaction between MOC and melanoidins. Deeper studies are required to understand and evaluate those factors that influence MOC efficiency so that the coagulation–flocculation process can be optimized. Full article

This study aimed to evaluate the fatty acid (FA) profile in milk from commercial farms with varying pasture levels in the diet during spring and fall, and to investigate the physical and chemical properties of butter to assess the impact of FAs on technological and nutritional properties. Milk sampling was conducted biweekly from six farms, categorized into high (HP) and low (LP) pasture treatments based on pasture intake: >60% and <35%, respectively. Butter was made from a pasture-based system (GRZ) and a confined system (C). No differences were observed in milk fat percentage between HP and LP in either season. High pasture had 85–66% more conjugated linoleic acid (CLA, p = 0.01), 74–48% more trans-vaccenic acid (TVA, p = 0.01), and 21–15% more branched-chain FAs (BCFAs, p = 0.006) than LP in spring and fall, respectively. In fall, butter from C had lower saturated FAs (SFAs, p = 0.005), higher unsaturated FAs (UFA, p = 0.008), and a lower spreadability index (SI, p = 0.005) than GRZ, resulting in softer butter. In conclusion, HP in both seasons had higher contents of FAs considered healthy for consumers compared to LP. Contrary to expectations, in fall, C showed higher UFAs and lower SFAs in butter, leading to better technological characteristics than GRZ. Full article

Currently, international trade has evolved into international production fragmentation captured in GVCs. Countries must enhance intermediate exports in comparative advantage sectors to increase their trade in value-added (TVA) in global production chains. However, traditional measurements of revealed comparative advantage (RCA) based on gross exports need to be updated due to overvaluation, double counting, and implicit distortions in international trade. This study uses a new comparative advantage measure, “new revealed symmetric comparative advantage” (NRSCA). Using a dynamic General Method of Moment (GMM) approach, we investigate the role of comparative advantage in driving TVA regarding backward and forward linkages and examine the impact of the COVID-19 pandemic. We use data from the current Asian Development Bank multi-regional input–output database for 2010–2020. Our findings reveal that comparative advantage significantly impacted international TVA, along with the support of quality institutional services in each country. Implementing a new comparative advantage measure, NRSCA, provided accurate estimation results to overcome the overvaluation problem. Moreover, the COVID-19 pandemic disrupted value-added trade. Full article

Autonomous underwater vehicles (AUVs) have been widely used in ocean missions. When they fail in the ocean, it is important to predict their trajectory. Existing methods rely heavily on historical trajectory data while overlooking the influence of the ocean environment on an AUV’s trajectory. At the same time, these methods fail to use the dependency between variables in the trajectory. To address these challenges, this paper proposes an AUV trajectory prediction model known as the nonlinear Kepler optimization algorithm–bidirectional long short-term memory–time-variable attention (NKOA-BiLSTM-TVA) model. This paper introduces opposition-based learning during the initialization process of the KOA and improves the algorithm by incorporating a nonlinear factor into the planet position update process. We designed an attention mechanism layer that spans both time and variable dimensions, called TVA. TVA can extract features from both the time and variable dimensions of the trajectory and use the dependency between trajectory variables to predict the trajectory. First, the model uses a convolutional neural network (CNN) to extract spatial features from the trajectory. Next, it combines a BiLSTM network with TVA to predict the AUV’s trajectory. Finally, the improved NKOA is used to optimize the model’s hyperparameters. Experimental results show that the NKOA-BiLSTM-TVA model has an excellent parameter optimization effect and higher prediction accuracy in AUV trajectory prediction tasks. It also achieves excellent results in ship trajectory prediction. Full article

2-phenylethanol (2-PE) is a valuable aromatic alcohol with diverse applications in cosmetics, food, beverages, and pharmaceutical industries. Currently, 2-PE is produced either through chemical synthesis or by extraction from plant materials. However, both conventional production methods have their own limitations. Therefore, there is a need for more eco-friendly and cost-effective approaches to produce natural 2-PE. Biotechnological routes, particularly microbial fermentations, hold promise for natural 2-PE production, especially when using low-cost substrates. In this study, 2-PE was produced by de novo synthesis via the shikimate pathway, using the yeast Yarrowia lipolytica in a medium composed of sugar beet molasses (SBM) and yeast extract (YE) as carbon and nitrogen sources, respectively. A genetically engineered strain was generated, in which the SUC2 gene was transformed, expressing the invertase enzyme, enabling Y. lipolytica to efficiently utilize SBM as a cost-effective substrate. A central composite design allowed for the optimization of the concentrations of the carbon and nitrogen sources, resulting in approximately 0.71 g_(2-PE)/L_{(culture medium)}. The results obtained highlight the potential of utilizing SBM as a low-cost substrate for 2-PE production, advancing biotechnological approaches in fragrance synthesis. Full article

Tower fatigue and strength are crucial operational concerns of floating offshore wind turbines (FOWTs) due to the escalation of the vibration phenomena observed on these structures as compared to land-based ones. FOWT towers are excited by wave and wind polyperiodic disturbances yielding continual transient states of structural vibration that are challenging for vibration mitigation systems. Thus, the paper investigates a novel implementation of nonlinear optimal-based vibration control solutions for the full-scale, tension leg platform (TLP)-based, NREL 5MW wind turbine tower-nacelle model with a 10-ton tuned vibration absorber (TVA), equipped with a magnetorheological (MR) damper, located at the nacelle. The structure is subjected to excessive wave and wind excitations, considering floating platform motions derived from model experiments in a wave tank. The MR damper operates simultaneously with an electromagnetic force actuator (forming a hybrid TVA) or independently (a semiactive TVA). The study includes both actuators’ nonlinearities and dynamics, whereby the former are embedded in the Hamilton-principle-based nonlinear control solutions. The TVA is tuned either to the NREL 5MW tower-nacelle 1st bending mode frequency (TVA-TN) or to the TLP surge frequency (TVA-TLP). The optimal control task was redeveloped concerning the TVA stroke and transient vibration minimisation, including the implementation of the protected structure’s acceleration and relative displacement terms, as well as the nonzero velocity term in the quality index. The regarded model is embedded in a MATLAB/Simulink environment. On the basis of the obtained results, the TVA-TN solution is by far superior to the TVA-TLP one. All the regarded TVA-TN solutions provide a tower deflection safety factor of ca. 2, while reference systems without any vibration reduction solutions or with a passive TVA-TLP are at risk of tower structural failure as well as the hybrid TVA-TLP system. The obtained TVA stroke reductions of 25.7%/22.0% coincide with 3.6%/10.3% maximum tower deflection reductions for the semiactive/hybrid TVA-TN case (respectively) with regard to the previously developed approaches. Moreover, these reductions are obtained due to the sole control algorithm enhancement; thus, no additional resources are necessary, while this attainment is accompanied by a reduction in the required MR damper force. The lowest obtained TVA stroke amplitude of 1.66 m is guaranteed by the newly introduced semiactive control. Its hybrid equivalent ensures 8% lower primary structure deflection amplitude and reduced nacelle acceleration levels thanks to the utilisation of the force actuator of the relatively low power (ca. 6 kW); the trade-off is an increased TVA stroke amplitude of 2.19 m, which, however, is the lowest among all the tested hybrid solutions. The analysed reference passive TVA systems, along with a modified ground-hook hybrid solution, can hardly be implemented in the nacelle (especially along the demanding side–side direction). The latter, being the well-proven hybrid solution for steady-state tower deflection minimisation, yielded unsatisfactory results. The achievements of the study may be used for an effective design of a full-scale vibration reduction system for the TLP-based floating wind turbine structure. Full article

Mycotoxins have been linked to adverse health impacts, including liver cancer and kidney diseases. The objectives of the current study were to evaluate the dietary exposure of Lebanese adults to multi-mycotoxins (aflatoxin B1 (AFB1), aflatoxin M1 (AFM1), ochratoxin A (OTA), ochratoxin B (OTB), deoxynivalenol (DON), T-2 and HT-2) and to assess their associated health risks. Hence, a nationally representative sample of 449 participants aged 18-64 years old were interviewed to obtain their socio-demographic characteristics, food consumption data and exposure estimates. A food frequency questionnaire and 24 h-recall were used to collect data. The concentration of mycotoxins in all foods consumed by the participants was collected from previous national published studies. The estimated daily intake (EDI), the hazard quotient (HQ) and the margin of exposure (MOE) were calculated. The total exposure to AFB1, AFM1, OTA and DON was 1.26, 0.39, 4.10 and 411.18 ng/kg bw/day, respectively. The MOE to AFB1, AFM1, OTA and DON in the Lebanese food basket was 316, 1454, 3539 and 510, respectively, indicating high health-related risks. Per food items, the MOE to AFB1 was below 10,000 in cereals (466.5), mainly in rice (827.9) and Burgul (4868.5). Similarly, the MOE to OTA in cereals was 1439, in which bread (4022), rice (7589) and bulgur (7628) were considered unsafe. Moreover, the MOE to DON in cereals (605) is alarming, especially in bread (632) and manakesh (6879). The MOE to AFM1 in dairy products was 1454, indicating health-related risks with a focus on yogurt (9788) and labneh (8153). As for the herbs/spices group and traditional dishes, the MOE to AFB1 was relatively lower than 10,000 (3690 and 1625, respectively), with a focus on thyme (2624) and kishik (3297), respectively. It is noteworthy that the MOE to DON and the MOE to OTA in traditional foods and coffee were lower than 10,000 (8047 and 8867, respectively). All hazard quotient (HQ) values were below 1, except the HQ value of milk and dairy products (1.96). The intake of some food groups varied between age categories, corresponding to differences in EDI between them. Thus, it is essential to put control measures in place to decrease the contamination and exposure to mycotoxins by Lebanese consumers. Full article

Colorimetric whole-cell biosensors are natural or genetically engineered microorganisms utilized to detect target molecules and ions as indicators of pollutants and biological activity in the environment. Upon detection, within specific concentration ranges which vary depending on the microorganism and its genetic circuitry among other factors, these sensors produce pigments which can be detected with the human eye past certain thresholds and quantified using simple analytical techniques, namely spectrophotometry. These sensors, which can be rendered portable through lyophilization and other methods, provide valuable and reliable substitutes of more demanding analytical ex situ techniques. The insights gained from this review can highlight technological progress in the field and contribute to the identification of potential opportunities afforded by these advancements. Full article

Optimal reinsurance problems under the risk measures, such as Value-at-Risk ($\mathrm{VaR}$) and Tail-Value-at-Risk ($\mathrm{TVaR}$), have been studied in recent literature. However, losses based on $\mathrm{VaR}$ may be underestimated and $\mathrm{TVaR}$ allows us to account better for catastrophic losses. In this paper, we propose a new family of flexible risk measures denoted by $\mathrm{LVaR}$, which is a weighted combination of $\mathrm{VaR}$ and $\mathrm{TVaR}$. Based on the new risk measures, we deal with the optimal reinsurance problem by minimizing the $\mathrm{LVaR}$ of the total risks of an insurer when two types of constraints for reinsurer’s risk exposure are considered. The results indicate that the two-layer reinsurance is always an optimal reinsurance policy with both types of constraints. Also, we find that the optimal reinsurance policy depends on the confidence level, the weight coefficient, the safety loading, the tolerance level, as well as the relations between them. Finally, we illustrate the results by numerical examples and compare them with the results in Lu et al. Full article

The goal of this study was to investigate the age-related differences in testosterone concentration and its relation to testicular biometrics, testicular blood flow, and fertility in alpacas (Vicugna pacos). Fifteen alpaca males with different ages (young (YM; ~12–14 mo.), n = 5; intermediate (IM; ~24 mo.), n = 5; and old (OM; ≥36 mo.), n = 5) were enrolled in the study. Blood samples were taken from each alpaca male and the circulating plasmatic testosterone concentration (TC; ng/mL) was determined using ELISA analysis. The testicular traits related to bio-morphometric parameters (the length (L), width (W), area (A), and volume (TV)) were assessed using B-mode ultrasonography. Pulse-wave/power Doppler ultrasonography was used to obtain the circulatory dynamic values (testicular hemodynamics) before the beginning of natural service mating. Significant differences were observed in TC among the age groups, increasing as the age of the males increased (2.47 ± 0.31, 8.45 ± 1.53, and 22.66 ± 2.15 for YM, IM, and OM, respectively; p < 0.05); however, no differences were observed regarding the testicular B-mode ultrasonographic parameters (L, W, and A) (p > 0.05). Positive correlations were observed between TV and testicular L, W, and A (r = 0.96, r = 0.95, and r = 0.96, respectively; p ≤ 0.001). Pulse-wave-Doppler-derived parameters such as the pulsatility index (PI) and the resistive index (RI), as well as the total vascularity area (TVA) assessed by power Doppler, were similar in all of the age groups studied (p > 0.05). General linear model (GLM) analysis showed a relationship between TC and TV (OR = 0.95; p = 0.04), as well as between TC and TVA (OR = 0.99; p= 0.02). Finally, no differences were observed regarding the pregnancy rate among the different age groups (p > 0.05). In conclusion, TC increased as the age of the alpaca males increased. Although TC was related to TV and TVA, the pregnancy rates obtained from individuals belonging to the different age groups were similar, indicating that TC, TV, and TVA were not determining factors in assessing the potential age-related fertility differences in alpaca males. Full article

The potential for plant-origin essential oils to modulate rumen functions for reducing bio-hydrogenation of fatty acids and methane production has been a significant area of research in recent times. This study investigated the effects supplementation of garlic (Allium sativum) essential oils have on in vitro bio-hydrogenation of fatty acids, methanogenesis and fermentation characteristics of total mixed ration in buffalo with the aim of enhancing conjugated linoleic acid (CLA) content in animal products as well as reducing environmental pollution. Allium sativum (AS) essential oils were examined at four levels [0 (Control), 33.33 µL (AS-1), 83.33 µL (AS-2) and 166.66 µL (AS-3) per litre of buffered rumen fluid] in a radio-frequency based automatic gas production system (ANKOM-RF). Two bottles per treatment per run over two incubation runs were undertaken to gain representative results. Oats hay and concentrate mixture (1:1) was used as a substrate (500 ± 5 mg) and incubated with 60 mL of buffered rumen fluid in 250 mL ANKOM bottles fitted with automatic an gas recording system at 39 °C for 24 h, following standard in vitro gas production protocols. The results demonstrated a reduction (p < 0.01) in lipid bio-hydrogenation, measured by lowered saturated fatty acids and enhanced unsaturated fatty acids on the supplementation of AS essential oils, irrespective of the dose levels. Moreover, the increased (p < 0.01) production of trans vaccenic (trans C18:1) acid (TVA) following graded dose supplementations of the AS essential oils increased the production of conjugated linoleic acids (CLA) in animal products. Although, reduced methane production (p < 0.01) was evidenced, the decrease in total gas production and feed digestibility (TDDM) demonstrated the strong antimicrobial properties of AS at all dose levels. The study reveals that the Allium sativam (Garlic) essential oils have the potential to be an agent for the reduction of the rumen biohydrogenation of fatty acids and methanogenesis. However, in vivo examination is necessary to validate the findings and confirm its suitability for use as an additive to enhance nutraceutical and organoleptic properties in animal products. Full article

Laser Induced-Thermionic Vacuum Arc (LTVA) technology was used for depositing uniform intermetallic CoNi thin films of 100 nm thickness. LTVA is an original deposition method using a combination of the typical Thermionic Vacuum Arc (TVA) system and a laser beam provided by a QUANTEL Q-Smart 850 Nd:YAG compact Q-switched laser with a second harmonic module. The novelty is related to the simultaneous deposition of a bi-component metallic thin film using photonic processes of the laser over the plasma deposition, which improves the roughness but also triggers the composition of the deposited thin film. Structural analysis of the deposited thin films confirms the formation of face-centered cubic (fcc) as the main phase CoNi and hexagonal Co₃Ni as the minority phase, observed mainly using high-resolution transmission electron microscopy. The magneto-optical measurements suggest an isotropic distribution of the CoNi alloy thin films for the in-plan angular rotation. From the low coercive field of H_c = 40 Oe and a saturation field at 900 Oe, the CoNi thin films obtained by LTVA are considered semi-hard magnetic materials. Magnetic force microscopy reveals spherical magnetic nanoparticles with mean size of about 40–50 nm. The resistivity was estimated at $\rho$ = 34.16 μΩ cm, which is higher than the values for bulk Co and Ni. Full article

Violacein and deoxyviolacein are bis-indole pigments synthesized by a number of microorganisms. The present study describes the biosynthesis of a mixture of violacein and deoxyviolacein using a genetically modified Y. lipolytica strain as a production chassis, the subsequent extraction of the intracellular pigments, and ultimately their purification using column chromatography. The results show that the optimal separation between the pigments occurs using an ethyl acetate/cyclohexane mixture with different ratios, first 65:35 until both pigments were clearly visible and distinguishable, then 40:60 to create a noticeable separation between them and recover the deoxyviolacein, and finally 80:20, which allows the recovery of the violacein. The purified pigments were then analyzed by thin-layer chromatography and nuclear magnetic resonance. Full article