Search Results (4,352)

The overexpression of the epidermal growth factor receptor (EGFR) in certain types of prostate cancers and glioblastoma makes it a promising target for targeted radioligand therapy. In this context, pairing an EGFR-targeting peptide with the emerging theranostic pair comprising the Auger electron emitter cobalt-58m (^58mCo) and the Positron Emission Tomography-isotope cobalt-55 (⁵⁵Co) would be of great interest for creating novel radiopharmaceuticals for prostate cancer and glioblastoma theranostics. In this study, GE11 (YHWYGYTPQNVI) was investigated for its EGFR-targeting potential when conjugated using click chemistry to N1-((triazol-4-yl)methyl)-N1,N2,N2-tris(pyridin-2-ylmethyl)ethane-1,2-diamine (TZTPEN). This chelator is suitable for binding Co²⁺ and Co³⁺. With cobalt-57 (⁵⁷Co) serving as a surrogate radionuclide for ^55/58mCo, the novel GE11-TZTPEN construct was successfully radiolabeled with a high radiochemical yield (99%) and purity (>99%). [⁵⁷Co]Co-TZTPEN-GE11 showed high stability in PBS (pH 5) and specific uptake in EGFR-positive cell lines. Disappointingly, no tumor uptake was observed in EGFR-positive tumor-bearing mice, with most activity being accumulated predominantly in the liver, gall bladder, kidneys, and spleen. Some bone uptake was also observed, suggesting in vivo dissociation of ⁵⁷Co from the complex. In conclusion, [⁵⁷Co]Co-TZTPEN-GE11 shows poor pharmacokinetics in a mouse model and is, therefore, not deemed suitable as a targeting radiopharmaceutical for EGFR. Full article

Micro-nano plastics (MNPs) are emerging environmental and food contaminants that are raising serious health concerns. Due to the polycontamination of the food web with environmental pollutants (EPs), and now MNPs, the co-ingestion of EPs and MNPs is likely to occur, and the potential synergistic effects of such co-ingestions are completely unstudied. In this study, we therefore sought to determine the effects of the two model EPs, arsenic and boscalid, on the uptake and toxicity of two model MNPs, 25 and 1000 nm polystyrene (PS-25 and PS-1000), and vice versa, employing a triculture small intestinal epithelium model combined with simulated digestion. In 24 h triculture exposures, neither MNPs, EPs, nor MNPs + EPs caused significant toxicity. The presence of PS-25 significantly increased arsenic uptake (from 0.0 to 5.8%, p < 0.001) and translocation (from 5.2 to 9.8%, p < 0.05) but had no effect on boscalid uptake or translocation, whereas PS-1000 had no effect on the uptake or translocation of either EP. The uptake of both PS MNPs was also increased by EPs, rising from 10.6 to 19.5% (p < 0.01) for PS-25 and from 4.8 to 8.5% (p < 0.01) for PS-1000. These findings highlight the need for further studies to assess MNP-EP interactions and possible synergistic adverse health impacts. Full article

The microwave polarization index (PI), defined as the difference between vertically polarized (V-pol) and horizontally polarized (H-pol) brightness temperature divided by their average, is independent of land surface temperature. Since soil emission is stronger at V-pol than H-pol and vegetation attenuates this polarized soil signal primarily because of liquid water stored in vegetation tissue, a lower PI will be indicative of more water in vegetation if vegetation emits a mostly unpolarized signal and changes in soil moisture within the emitting depth are small (like during periods of drought) or accommodated by averaging over long periods. We hypothesize that the L-band PI will reveal diurnal changes in vegetation water related to whether plants have adequate soil water. We compare 6 a.m. and 6 p.m. L-band PI from NASA’s Soil Moisture Active Passive (SMAP) satellite to the evaporative stress index (ESI) in the U.S. Corn Belt during the growing season. When $\mathrm{ESI}<0$ (there is not adequate plant-available water, also called plant water stress), the L-band PI is not significantly different at 6 a.m. vs. 6 p.m. On the other hand, when $\mathrm{ESI}\ge 0$ (no plant water stress), the L-band PI is greater in the evening than in the morning. This diurnal behavior can be explained by transpiration outpacing root water uptake during daylight hours (resulting in a decrease in vegetation water from 6 a.m. to 6 p.m.) and continued root water uptake overnight (that recharges vegetation water) only when plants have adequate soil water. Consequently, it may be possible to use L-band PI to identify plant water stress in the Corn Belt. Full article

Background: Anesthesia can significantly impact positron emission tomography (PET) neuroimaging in preclinical studies. Therefore, understanding these effects is crucial for accurate interpretation of the results. In this experiment, we investigate the effect of [¹⁸F]-labeled glucose analog fluorodeoxyglucose ([¹⁸F]FDG) uptake in the brains of rats anesthetized with two commonly used anesthetics for rodents: isoflurane, an inhalation anesthetic, and Hypnorm–Dormicum, a combination injection anesthetic. Materials and Methods: Female adult Sprague Dawley rats were randomly assigned to one of two anesthesia groups: isoflurane or Hypnorm–Dormicum. The rats were submitted to dynamic [¹⁸F]FDG PET scan. The whole brain [¹⁸F]FDG standard uptake value (SUV) and the brain voxel-based analysis were performed. Results: The dynamic [¹⁸F]FDG data revealed that the brain SUV was 38% lower in the isoflurane group after 40 min of image (2.085 ± 0.3563 vs. 3.369 ± 0.5577, p = 0.0008). In voxel-based analysis between groups, the maps collaborate with SUV data, revealing a reduction in [¹⁸F]FDG uptake in the isoflurane group, primarily in the cortical regions, with additional small increases observed in the midbrain and cerebellum. Discussion and Conclusions: The observed differences in [¹⁸F]FDG uptake in the brain may be attributed to variations in metabolic activity. These results underscore the necessity for careful consideration of anesthetic choice and its impact on neuroimaging outcomes in future research. Full article

Cover crops have many simultaneous roles that enhance the sustainability of agriculture compared to leaving land fallow in arable systems. In high rainfall climates, an important role of cover crops is to sequester nutrients, protecting them from loss to the environment. In many livestock intensive regions, organic manures are applied in autumn with land left fallow over winter and then a cash crop is planted in the spring. This practice of extended fallow, combined with the poorly synchronised application of nutrients to cash crop nutrient demand, further adds to the potential for large losses of nutrients specifically nitrogen (N) to the environment through leaching and volatilization. Therefore, if cover crops could respond to these nutrients through increased biomass growth and nutrient uptake, they could reduce these losses. Therefore, it is important to choose the correct species which grows adequately to deliver these potential benefits. In the region this study is conducted, Northern Ireland, there has been little research to investigate not only what optimum cover crop species are but also what species could respond to additional nutrients in the slurry. This study comprises two experiments, in consecutive years, including a combination of three factors: cover crop species (five species; a mixture and a control representing fallow); pig slurry. Consequently, it was found that with good growing conditions, in the trial year of 2018/19, species such as forage rape and tillage radish could significantly (p < 0.05) increase the amount of N contained in their biomass and the soil (to 15 cm) by 70 and 63%, respectively, in response to slurry. Alternatively, when slurry was applied to fallow land (conventional practice), low amounts (14 and 0% in the two trial years, respectively) of the N from the slurry were detected in weed biomass or retained in the soil. This demonstrates a large loss of N from the system. Thus, the integration of responsive cover crops with slurry is a better practice to abate N loss than conventional practice, if slurry applications can not be delayed. Full article

Background and Objectives: Three respiratory syncytial virus (RSV) vaccines have been recently made available for older adults. Understanding the principal characteristics of the first vaccine-takers can pave the way for a successful vaccination campaign. The objective of this study was to explore the sociodemographic and clinical characteristics of the first Italian users of an adjuvanted RSV vaccine and their attitudes towards RSV and vaccination. Materials and Methods: This cross-sectional study was conducted in 2024 in Liguria (Italy). Individuals aged ≥60 years with no contraindications to the adjuvanted vaccine RSVPreF3 OA were eligible. Following vaccination, subjects filled in a questionnaire, which comprised items on sociodemographic and clinical characteristics, attitudes towards RSV and RSV vaccination and a vaccination trust indicator (VTI). Results: A total of 453 vaccinees completed the survey. Their mean age was 74.9 ± 8.0 years, and 50.6% were males. Nine of ten (89.2%) individuals had ≥1 co-morbidity, of which cardiovascular conditions (70.4%), respiratory diseases (27.6%) and diabetes (18.5%) were the most common. Uptake of the routine vaccines was high: 91.2% and 98.7% received the 2023/2024 season influenza and ≥2 COVID-19 vaccines, respectively. The most common reasons for the current RSV vaccination were general practitioner advice (43.9%), followed by the willingness to be protected against (20.8%) and feelings of being at risk (16.6%) of RSV. The average VTI score was 91.5%, suggesting high trust in vaccines. More positive attitudes towards RSV vaccination were observed (p < 0.01) among subjects who received more COVID-19 vaccine doses, whose reasons for the current RSV vaccination were the willingness to be protected or to be in good health and the feeling of being at risk for RSV. Conclusions: The first Italian users of the novel RSVPreF3 OA vaccine were represented by high-risk individuals with a comparatively high prevalence of co-morbidities, high uptake of the seasonal respiratory vaccines and high trust in immunization. Full article

This experiment examined the effects of blending bottom ash produced after combusting dry livestock manure (BACL, 2–4 mm particle) as a soil amendment on the physicochemical properties of the root zone and growth response of creeping bentgrass in sandy soil. The treatments were designed as follows: control [100% sand], 3% BACL (3% BACL + 97% sand), 5% BACL (5% BACL + 95% sand), 7% BACL (7% BACL + 93% sand), and 10% BACL (10% BACL + 90% sand). Although BACL improved the soil physical properties, such as the capillary porosity, total porosity, and hydraulic conductivity, it reduced the cation exchangeable capacity. The BACL treatments increased the pH, EC, Av-P₂O₅, and Ex-K compared to the control. The turf color index, chlorophyll content, shoot length, clipping yield, and shoot dry weight after the BACL treatments were similar to the control. The growth and nutrient uptake of the roots in the BACL treatment were higher than those of the control. The BACL application amount was positively correlated with the capillary porosity and total porosity of the root zone (p ≤ 0.01) and with the growth and nutrient levels of the roots (p ≤ 0.05). These results suggest that applying BACL as a soil amendment enhanced the uptake of phosphorus and potassium in the roots of creeping bentgrass by improving the soil porosity in the root zone and by supplying phosphate and potassium. Full article

Intercropping has the potential to improve phosphorus (P) uptake and crop growth, but the potential benefits and relative contributions of root morphology and arbuscular mycorrhizal fungi (AMF) colonization are largely unknown for the intercropping of rice and soybean under dry cultivation. Both field and pot experiments were conducted with dry-cultivated rice (Oryza sativa L.) and soybean (Glycine max L. Merr.) grown alone or intercropped under two P levels. Two root separation modes between rice and soybean were employed to explore the contribution of AMF association and root plasticity on P uptake in intercrops. The results showed that rice/soybean intercropping resulted in a notable increase in the total biomass and yield compared to monoculture in the field. In the potted experiment, compared to the plastic root separation treatment (PS), the no root separation treatment (NS) increased the total biomass and P uptake by 9.4% and 19.9%, irrespective of the P levels. This was primarily attributable to a considerable enhancement in biomass and phosphorus uptake in soybean by 40.4% and 49.7%, which offset a slight decline in the rice of NS compared to PS by 26.8% and 18.0%, respectively. The results of random forest analysis indicate that the P uptake by the dominant species, soybean, was mainly contributed by root morphology, while rice was more dependent on AMF colonization in the intercropping system. Therefore, dry-cultivated rice/soybean intercropping enhances P uptake and productivity by leveraging complementary belowground strategies, with soybean benefiting primarily from root morphological adjustments and rice relying more on arbuscular mycorrhizal fungi colonization. Full article

Hydrogels are a viable option for biomedical applications due to their biocompatibility, biodegradability, and ability to incorporate various healing agents while maintaining their biological efficacy. This study focused on the preparation and characterization of novel hybrid hydrogels enriched with the natural algae compound Ulvan for potential use in wound dressings. The characterization of the hydrogel membranes involved multiple methods to assess their structural, mechanical, and chemical properties, such as pH measurements, swelling, moisture content and uptake, gel fraction, hydrolytic degradation, protein adsorption and denaturation tests, rheological measurements, SEM, biocompatibility testing, and scratch wound assay. The hydrogel obtained with a higher concentration of Ulvan (1 mg/mL) exhibited superior mechanical properties, a swelling index of 264%, a water content of 55%, and a lower degradation percentage. In terms of rheological properties, the inclusion of ULV in the hydrogel composition enhanced gel strength, and the Alginate + PVA + 1.0ULV sample demonstrated the greatest resistance to deformation. All hydrogels exhibited good biocompatibility, with cell viability above 70% and no obvious morphological modifications. The addition of Ulvan potentiates the regenerative effect of hydrogel membranes. Subsequent studies will focus on encapsulating bioactive compounds, investigating their release behavior, and evaluating their active biological effects. Full article

Background/Objectives: Cervical cancer is a significant global health concern, with high incidence and mortality rates, especially in less-developed regions. [¹⁸F]FDG PET/CT is now indicated at various stages of management, but its analysis is essentially based on SUVmax, a measure of [¹⁸F]FDG uptake. Radiomics, by extracting a multitude of parameters, promises to improve the diagnostic and prognostic performance of the examination. However, studies remain heterogeneous, both in terms of patient numbers and methods, so a synthesis is needed. Methods: This systematic review was conducted following PRISMA-P guidelines and registered in PROSPERO (CRD42024584123). Eligible studies on PET/CT radiomics in cervical cancer were identified through PubMed and Scopus and assessed for quality using the Radiomics Quality Score (RQS v2.0), with data extraction focusing on study design, population characteristics, radiomic methods, and model performances. Results: The review identified 22 studies on radiomics in cervical cancer, 19 of which focused specifically on locally advanced cervical cancer (LACC) and assessed various clinical outcomes, such as survival, relapse, treatment response, and lymph node involvement prediction. They reported significant associations between prognostic indicators and radiomic features, indicating the potential of radiomics to improve the predictive accuracy for patient outcomes in LACC; however, the overall quality of the studies was relatively moderate, with a median RQS of 12/36. Conclusions: While radiomic analysis in cervical cancer presents promising opportunities for survival prediction and personalized care, further well-designed studies are essential to provide stronger evidence for its clinical utility. Full article

Introduction: Follicular lymphoma (FL) is a heterogeneous disease, and identifying high-risk patients early is crucial for optimal management. This study aimed to evaluate the prognostic significance of interim positron emission tomography/computed tomography (PET/CT) in newly diagnosed FL patients undergoing first-line treatment. Methods: This single-center, retrospective study included 103 patients with newly diagnosed FL who underwent interim PET/CT. The primary endpoint was progression-free survival (PFS). The prognostic value of positive interim PET/CT, Deauville scores, and maximum standardized uptake values (SUVmax) were analyzed among other clinical features. Results: A total of 30 patients (29.1%) were deemed interim PET/CT-positive. The interim PET/CT-positive group demonstrated a significantly shorter median PFS compared to the interim PET/CT-negative group (17 months vs. not reached, respectively; p < 0.0001). Similarly, patients with Deauville scores of 1–3 had better PFS, with median survival not yet reached, while those with higher Deauville scores exhibited poorer progression-free survival and a median survival of 18 months. Notably, patients with an interim PET/CT SUVmax exceeding 3.365 experienced significantly shorter median survival compared to those with lower values. Discussion: Our findings support the use of interim PET/CT as a prognostic tool in FL. These results are consistent with other studies, although some debate exists regarding the optimal PET/CT metric. Further research is needed to validate these findings and explore personalized treatment strategies based on interim PET/CT results. Full article

Colombian goldenberries that do not reach sufficient quality for export are exposed to waste, so the search for processes that provide added value while guaranteeing the conservation of this fruit is paramount. Thus, snacks by vacuum frying from goldenberries (Physalis peruviana L.) with low export quality were made. Goldenberry slices previously subjected to ultrasound-assisted osmotic dehydration were used for this purpose. Response surface methodology with different levels of temperature (110 °C and 130 °C), vacuum pressure (0.3 bar and 0.5 bar), and time (2 min and 6 min) was used to optimize the process. At optimal vacuum frying conditions (i.e., 108 °C, 0.5 bar, and 5.5 min), snacks with lower (p ≤ 0.05) oil content were produced, compared to atmospheric frying chips. The optimized snacks had 9% oil, 7% moisture, ΔE of 13 (with respect to fresh fruit), a_w of 0.3, and hardness of 14 N. The kinetics and modeling of moisture loss and oil uptake were performed under optimal conditions, obtaining the best fit with the Page (R² = 99%) and the first-order (R² = 96%) models, respectively. There was a clear correlation between oil uptake and moisture loss, as the highest oil retention in the product took place when the product had lost the greatest amount of water; therefore, the low initial moisture in the product due to pretreatment resulted in lower oil uptake in it. The obtained goldenberry snack showed adequate physicochemical properties, and the pretreatment yielded a product with much healthier characteristics (i.e., lower oil content, and therefore, a lower caloric intake); so, the proposed process could represent an alternative to the processing of low-export quality Colombian goldenberries. Full article

Hypoxia, a phenomenon that occurs when the oxygen level in tissues is lower than average, is commonly observed in human solid tumors. For oncological treatment, the hypoxic environment often results in radioresistance and chemoresistance. In this study, a new multifunctional oxygen carrier, carboxymethyl hexanoyl chitosan (CHC) nanodroplets decorated with perfluorohexane (PFH) and superparamagnetic iron oxide (SPIO) nanodroplets (SPIO@PFH-CHC), was developed and investigated. PFH-based oxygen carriers can augment oxygenation within tumor tissues, thereby mitigating radioresistance. Concurrently, oxygenation can cause deoxyribonucleic acid (DNA) damage via oxygen fixation and consequently suppress cancer cell proliferation. Moreover, these pH-sensitive nanodroplets allow higher cellular uptake with minimal cytotoxicity. Two distinctive mechanisms of SPIO@PFH-CHC nanodroplets were found in this study. The SPIO nanoparticles of the SPIO@PFH-CHC nanodroplets can generate hydroxyl radicals (HO^•) and other reactive oxygen species (ROS), which is vital to chemodynamic therapy (CDT) via the Fenton reaction. Meanwhile, the higher X-ray absorption among these nanodroplets leads to a local energy surge and causes more extensive deoxyribonucleic acid (DNA) damage via oxygen fixation. This study demonstrates that low cytotoxic SPIO@PFH-CHC nanodroplets can be an efficient radiosensitizer for radiation therapy. Full article

Background/Objectives: Colon capsule endoscopy (CCE) is a non-invasive method for visualising the colon, but its clinical adoption has been slow. Although the COVID-19 pandemic reignited interest in CCE, its role in conventional gastrointestinal investigations remains unclear, leading to varied practices across Europe. This highlights the need for a comprehensive understanding of diverse approaches to CCE in clinical practice. Method: A web-based survey was conducted from January to July 2024, targeting European gastroenterologists and colorectal surgeons interested in capsule endoscopy through the International Capsule Endoscopy Research (iCARE) Group. The survey aimed to understand CCE application across Europe and investigate factors influencing its uptake. Results: Thirty-eight (n = 38) valid responses were received from 19 European countries. While 88% reported access to CCE, only 45% had local services readily available, and just 7% included CCE in national guidelines. The most common indication for CCE was for patients who declined or could not tolerate colonoscopy (30%), with 77% of CE specialists preferring its use in fit patients. Ease of access was significantly associated with service availability (p = 0.0358). Barriers to uptake included lack of reimbursement, insufficient knowledge, and limited use in research settings. Only 27% of specialists viewed CCE positively, while 57% had a negative perception. Conclusions: This study reveals the wide variation in CCE practices and critical factors influencing its uptake. Understanding common indications and patient groups is the key to guiding its future development, particularly as AI and telemedicine enhance its potential for rapid full digestive tract visualisation. Full article

Soil in the karst region usually features high geogenic cadmium (Cd) and limited available phosphorus (P). Appropriate phosphate amendment is crucial for alleviating Cd accumulation in food crops and reducing health risks. However, the interaction of Cd and P in geogenic Cd-rich soil-plant systems is poorly understood. In this study, a pot experiment was conducted to investigate the translocation of Cd in the soil-pepper system under different amendment rates of Ca(H₂PO₄)₂. The results showed that the biomass of pepper was not affected by the application rates of Ca(H₂PO₄)_2, even up to 0.45 g/kg, but was affected by the application of nitrogen and potassium fertilizers. High contents of total Cd (6.19 mg/kg) and bioavailable Cd (2.72 mg/kg, 44%) in the studied soils resulted in elevated Cd content in pepper, and it decreased in the order of root (8.18 mg/kg) > stem (4.89 mg/kg) > fruit (3.88 mg/kg). This indicates that pepper planted in the studied soils may present potential health risks. Furthermore, phosphate amendment neither influences the bioavailable Cd in rhizosphere soil nor Cd uptake and transport in pepper plants. The findings of this study highlight that monocalcium phosphate is not a suitable choice for reducing the accumulation of Cd in pepper fruits in the studied soil and that other remediation strategies are needed. Full article