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21 pages, 6277 KiB  
Article
Characterization and Adsorption Behavior of Newly Synthesized Aminated Cellulose with Jeffamine EDR148 Towards Ni(II), Cu(II), and Pb(II) Heavy Metal Ions
by Jawaher Y. Al Nawah and Amany S. El-Khouly
Polymers 2025, 17(2), 255; https://doi.org/10.3390/polym17020255 - 20 Jan 2025
Viewed by 398
Abstract
Industrial wastewater containing heavy metal ions presents serious economic risk to the environment. In this study, a novel compound of aminated cellulose with jeffamine EDR148 was prepared to improve cellulose’s adsorptive behavior towards metal ions. This study undertook a straightforward and efficient cellulose [...] Read more.
Industrial wastewater containing heavy metal ions presents serious economic risk to the environment. In this study, a novel compound of aminated cellulose with jeffamine EDR148 was prepared to improve cellulose’s adsorptive behavior towards metal ions. This study undertook a straightforward and efficient cellulose modification through homogeneous chlorination in N,N′-butylmethylimidazolium chloride to produce 6-deoxychlorocellulose (Cell-Cl), followed by a reaction with jeffamine EDR148 and ultimately resulting in the formation of aminated cellulose (Cell-Jef148). Structural and chemical characteristics of Cell-Cl and Cell-Jef148 were determined using different techniques. Various adsorption conditions were applied to evaluate the optimal adsorption conditions for the removal of Cu(II), Ni(II), and Pb(II) ions. Cell-Jef48 revealed a greater affinity and higher adsorption efficiency of 480.3, 420.5, and 463.2 mg/g for Cu(II), Ni(II), and Pb(II) ions, respectively. Different kinetics and adsorption isothermal models were studied to investigate the adsorption mechanism and interactions between Cell-Jef148 and metal ions. The results fitted the Langmuir and pseudo-second-order models. Corresponding to the Langmuir model, Cell-Jef148’s maximum adsorption capacities were 952.38, 609.76, and 769.23 mg/g for Cu(II), Ni(II), and Pb(II) ions, respectively, with a high correlation coefficient, R2, in the range of 0.99575–0.99855. The research results of this study support Cell-Jef148’s adsorption of heavy metal ions, and the regeneration of adsorbent highlights the potential applications of cellulose-based materials in wastewater treatment. Full article
(This article belongs to the Section Polymer Applications)
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<p>SEM images of (<b>a</b>) Pure-Cell, (<b>b</b>) Cell-Cl, and (<b>c</b>) Cell-Jef148.</p>
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<p>(<b>A</b>) FT-IR spectra of (a) Pure-Cell, (b) Cell-Cl, and (c) Cell-Jef148. (<b>B</b>). FT-IR spectra of Cell-Jef48 after adsorption of the metal ions, (a) Cu(II), (b) Ni(II), and (c) Pb(II).</p>
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<p><sup>13</sup>C CP MAS NMR spectra of Pure-Cell, Cell-CL, and Cell-Jef148.</p>
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<p>(<b>A</b>) X-ray diffraction of (a) Pure-Cell, (b) Cell-Cl, and (c) Cell-Jef148. (<b>B</b>) X-ray diffraction of Cell-Jef148 after the adsorption of metal ions (a) Cu(II), (b) Ni(II), and (c) Pb(II).</p>
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<p>TG graphs of Pure-Cell, Cell-Cl, and Cell-Jef148.</p>
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<p>Influence of pH on the adsorption of heavy metal ions.</p>
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<p>(<b>a</b>) Influence of temperature and (<b>b</b>) on the adsorption of heavy metal ions.</p>
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<p>(<b>a</b>) Influence of temperature and (<b>b</b>) on the adsorption of heavy metal ions.</p>
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<p>Influence of adsorbent dose on the adsorption of heavy metal ions.</p>
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<p>Influence of metal ion concentration on the adsorption of the heavy metal ions.</p>
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<p>Langmuir (<b>a</b>), Freundlich (<b>b</b>), and Temkin (<b>c</b>) isothermal models for the adsorption of Cu(II), Ni(II), and Pb(II).</p>
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<p>Linear plots of (<b>a</b>) pseudo-first-order and (<b>b</b>) pseudo-second-order models for adsorption of Cu(II), Ni(II), and Pb(II) metal ions onto Cell-Jef148.</p>
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<p>The proposal mechanism of adsorption of heavy metal ions onto Cell-Jef148.</p>
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<p>The percent uptake of Cell-Jef148 after several regeneration cycles.</p>
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<p>Preparation reactions of Cell-Cl and Cell-Jef148.</p>
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20 pages, 7294 KiB  
Article
Prelaunch Reflective Solar Band Radiometric Performance of JPSS-3 and -4 VIIRS
by Amit Angal, David Moyer, Xiaoxiong Xiong, Qiang Ji and Daniel Link
Remote Sens. 2024, 16(24), 4799; https://doi.org/10.3390/rs16244799 - 23 Dec 2024
Viewed by 349
Abstract
The Joint Polar Satellite System 3 (JPSS-3) and -4 (JPSS-4) Visible Infrared Imaging Radiometer Suite (VIIRS) instruments are the last in the series (S-NPP VIIRS launched in October 2011, JPSS-1 VIIRS launched in November 2017, and JPSS-2 VIIRS launched in November 2022) of [...] Read more.
The Joint Polar Satellite System 3 (JPSS-3) and -4 (JPSS-4) Visible Infrared Imaging Radiometer Suite (VIIRS) instruments are the last in the series (S-NPP VIIRS launched in October 2011, JPSS-1 VIIRS launched in November 2017, and JPSS-2 VIIRS launched in November 2022) of highly advanced polar-orbiting environmental satellites. Both instruments underwent a comprehensive sensor-level thermal vacuum (TVAC) testing at the Raytheon Technologies El Segundo facility to characterize the spatial, spectral, and radiometric aspects of the VIIRS sensor performance. This paper focuses on the radiometric performance of the 14 reflective solar bands (RSBs) that cover the wavelength range from 0.41 to 2.3 µm. Key instrument calibration parameters such as instrument gain, signal-to-noise ratio (SNR), dynamic range, and radiometric calibration uncertainty were derived from the TVAC measurements for both the primary and redundant electronics at three instrument temperature plateaus: cold, nominal, and hot. This paper shows that all the JPSS-3 and -4 VIIRS RSB detectors have been well characterized, with key performance metrics comparable to the previous VIIRS instruments on-orbit. The radiometric calibration uncertainty of the RSBs is within the 2% requirement, except in the case of band M1 of JPSS-4. Comparison of the radiometric performance to sensor requirements, as well as a summary of key instrument testing and performance issues, is also presented. Full article
(This article belongs to the Collection The VIIRS Collection: Calibration, Validation, and Application)
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<p>Schematic of the optical paths into the VIIRS rotating telescope assembly (RTA) and SDSM during solar observations. The angles and positions are not drawn to scale and are for illustrative purposes.</p>
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<p>Comparison of the on-orbit solar spectral irradiance profile and the SIS-100 radiance profile.</p>
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<p>Cartoon of the TVAC test chamber setup. The Blackbody calibration source (BCS), SVS, TMC-SIS, and SIS-100 sources are shown with respect to the instrument location within the chamber.</p>
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<p>(<b>a</b>–<b>c</b>) dn vs. L with the fits, fractional residuals, tau vs. radiance for band M4H from J3 TVAC. The blue dotted lines represent Lmin and Lmax and the pink dotted line represents Ltyp.</p>
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<p>(<b>a</b>–<b>c</b>) dn vs. L with the fits, fractional residuals, tau vs. radiance for band M4H from J4 TVAC. The blue dotted lines represent Lmin and Lmax and the pink dotted line represents Ltyp.</p>
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<p>(<b>a</b>–<b>c</b>) dn vs. radiance with the fits, fractional residuals, tau vs. radiance for band M4L from J3 TVAC. The blue dotted lines represent Lmin and Lmax and the pink dotted line represents Ltyp.</p>
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<p>(<b>a</b>–<b>c</b>) dn vs. radiance with the fits, fractional residuals, tau vs. radiance for band M4L from J4 TVAC. The blue dotted lines represent Lmin and Lmax and the pink dotted line represents Ltyp.</p>
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<p>(<b>a</b>–<b>c</b>) tau, c0/c1, and c2/c1 coefficients with 2-sigma error bars for band M4H from J3 TVAC.</p>
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<p>(<b>a</b>–<b>c</b>) tau, c0/c1, and c2/c1 coefficients with 2-sigma error bars for band M4L from J3 TVAC.</p>
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<p>(<b>a</b>–<b>c</b>) tau, c0/c1, and c2/c1 coefficients with 2-sigma error bars for band M4H from J4 TVAC.</p>
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<p>(<b>a</b>–<b>c</b>) tau, c0/c1, and c2/c1 coefficients with 2-sigma error bars for band M4L from J4 TVAC.</p>
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<p>(<b>a</b>,<b>b</b>) SNR vs. L for M4H and M4L from J3 TVAC.</p>
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<p>(<b>a</b>,<b>b</b>) SNR vs. L for M4H and M4L from J4 TVAC.</p>
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<p>(<b>a</b>,<b>b</b>) Normalized gain (band-averaged) versus the VNIR FPA and ASP temperatures for JPSS-3 VIIRS.</p>
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<p>(<b>a</b>,<b>b</b>) Normalized gain (band-averaged) versus the VNIR FPA and ASP temperatures for JPSS-4 VIIRS.</p>
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14 pages, 4415 KiB  
Article
A Green Computing Business Aggregation Strategy for Low Earth Orbit Satellite Networks
by Bo Wang, Jiaqi Lv, Dongyan Huang, Zelin Lu and Yuhang Fang
Sensors 2024, 24(24), 8184; https://doi.org/10.3390/s24248184 - 21 Dec 2024
Viewed by 645
Abstract
This paper proposes a green computing strategy for low Earth orbit (LEO) satellite networks (LSNs), addressing energy efficiency and delay optimization in dynamic and energy-constrained environments. By integrating a Markov Decision Process (MDP) with a Double Deep Q-Network (Double DQN) and introducing the [...] Read more.
This paper proposes a green computing strategy for low Earth orbit (LEO) satellite networks (LSNs), addressing energy efficiency and delay optimization in dynamic and energy-constrained environments. By integrating a Markov Decision Process (MDP) with a Double Deep Q-Network (Double DQN) and introducing the Energy–Delay Ratio (EDR) metric, this study effectively quantifies and balances energy savings with delay costs. Simulations demonstrate significant energy savings, with reductions of up to 47.87% under low business volumes, accompanied by a minimal delay increase of only 0.0161 s. For medium business volumes, energy savings reach 26.75%, with a delay increase of 0.0189 s, while high business volumes achieve a 4.36% energy reduction and a delay increase of 0.0299 s. These results highlight the proposed strategy’s ability to effectively balance energy efficiency and delay, showcasing its adaptability and suitability for sustainable operations in LEO satellite networks under varying traffic loads. Full article
(This article belongs to the Section Communications)
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<p>Layered architecture of LSNs showing control, business, and user layers.</p>
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<p>Business satellite load aggregation diagram.</p>
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<p>Business aggregation flow chart.</p>
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<p>Satellite simulation scene map.</p>
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<p>Performance of the recommended aggregation strategy.</p>
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<p>Energy–Delay Ratio (EDR) versus number of businesses for different optimization strategies.</p>
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12 pages, 10856 KiB  
Article
Multi-Resonant Full-Solar-Spectrum Perfect Metamaterial Absorber
by Zhe Shen and Junfan Ni
Nanomaterials 2024, 14(23), 1959; https://doi.org/10.3390/nano14231959 - 6 Dec 2024
Viewed by 553
Abstract
Currently, perfect absorption properties of metamaterials have attracted widespread interest in the area of solar energy. Ultra-broadband absorption, incidence angle insensitivity, and polarization independence are key performance indicators in the design of the absorbers. In this work, we proposed a metamaterial absorber based [...] Read more.
Currently, perfect absorption properties of metamaterials have attracted widespread interest in the area of solar energy. Ultra-broadband absorption, incidence angle insensitivity, and polarization independence are key performance indicators in the design of the absorbers. In this work, we proposed a metamaterial absorber based on the absorption mechanism with multiple resonances, including propagation surface plasmon resonance (PSPR), localized surface plasmon resonance (LSPR), electric dipole resonance (EDR), and magnetic dipole resonance (MDR). The absorber, consisting of composite nanocylinders and a microcavity, can perform solar energy full-spectrum absorption. The proposed absorber obtained high absorption (>95%) from 272 nm to 2742 nm at normal incidence. The weighted absorption rate of the absorber at air mass 1.5 direct in the wavelength range of 280 nm to 3000 nm exceeds 98.5%. The ultra-broadband perfect absorption can be ascribed to the interaction of those resonances. The photothermal conversion efficiency of the absorber reaches 85.3% at 375 K. By analyzing the influence of the structural parameters on the absorption efficiency, the absorber exhibits excellent fault tolerance. In addition, the designed absorber is insensitive to polarization and variation in ambient refractive index and has an absorption rate of more than 80% at the incident angle of 50°. Our proposed absorber has great application potential in solar energy collection, photothermal conversion, and other related areas. Full article
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<p>(<b>a</b>) Schematic diagram of the proposed metamaterial absorber. Side (<b>b</b>) and top (<b>c</b>) view of the absorber unit.</p>
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<p>(<b>a</b>) The absorption spectrum of the absorber under normal incidence. (<b>b</b>) The absorption spectrum on the scale of absorption rate from 0.75 to 1. (<b>c</b>) The distribution of solar energy and energy absorbed by the absorber at AM 1.5D. (<b>d</b>) Solar energy is absorbed and unabsorbed by the absorber at AM 1.5D.</p>
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<p>Thermal emission spectrum of the absorber (red) and the ideal blackbody (black) from 2.5 µm to 20 µm at 375 K (dotted line) and 425 K (solid line).</p>
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<p>Electric field (<b>a</b>,<b>d</b>,<b>f</b>,<b>h</b>) and magnetic field (<b>b</b>,<b>e</b>,<b>g</b>,<b>i</b>) distributions of the metamaterial absorber in the x-z plane at 332 nm, 532 nm, 940 nm, and 2400 nm, respectively. (<b>c</b>) Charge and electrical field line distributions in the x-y plane at the red line in (<b>a</b>). The green color framed by the black dotted lines in (<b>a</b>) represents two pairs of local electric fields.</p>
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<p>(<b>a</b>) Absorption spectrum of the absorber (red), and the absorber without the SiO<sub>2</sub> layer (gray), and without composite nanocylinder (blue). (<b>b</b>) The absorption spectrum of the absorbers with the number of TiN nanodisk layers from 1 to 4.</p>
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<p>(<b>a</b>–<b>d</b>) Electric field distributions of the metamaterial absorbers with numbers of TiN nanodisk layers from 1 to 4 in the x-z plane at 2400 nm.</p>
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<p>(<b>a</b>–<b>d</b>) are absorption spectrum under the four cases of different thicknesses of SiO<sub>2</sub> nanodisk (h<sub>1</sub>), TiN nanodisk (h<sub>2</sub>), TiN film (h<sub>3</sub>), and SiO<sub>2</sub> film (h<sub>4</sub>).</p>
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<p>Absorption spectrum map of the absorbers at different structure periods (<b>a</b>) and nanocylinder radius (<b>b</b>). The write dotted lines donate the resonance wavelengths.</p>
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<p>(<b>a</b>) The absorption spectrum map of the absorber at different polarization angles (0–90°) of incidence. Absorption spectrum map of the absorber to different angles (0–70°) of incidence in TM mode (<b>b</b>) and TE mode (<b>c</b>).</p>
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<p>The absorption spectrum of the absorber at ambient refractive indexes from 1 to 1.3.</p>
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27 pages, 9297 KiB  
Article
Integrating Connected Vehicles into IoT Ecosystems: A Comparative Study of Low-Power, Long-Range Communication Technologies
by Valentin Iordache, Marius Minea, Răzvan Andrei Gheorghiu, Florin Bădău, Angel Ciprian Cormoș, Valentin Alexandru Stan, Ion Nicolae Stăncel and Victor Stoica
Sensors 2024, 24(23), 7607; https://doi.org/10.3390/s24237607 - 28 Nov 2024
Viewed by 807
Abstract
Integrating road vehicles into broader Internet of Things (IoT) ecosystems is an important step in the development of fully connected and smart transportation systems. This research explores the potential of using communication technologies that achieve a balance between low-power and long-range (LPLR) capabilities [...] Read more.
Integrating road vehicles into broader Internet of Things (IoT) ecosystems is an important step in the development of fully connected and smart transportation systems. This research explores the potential of using communication technologies that achieve a balance between low-power and long-range (LPLR) capabilities while remaining cost-effective, specifically Bluetooth Classic BR-EDR, Bluetooth LE, ZigBee, nRF24, and LoRa—for Vehicle-to-Infrastructure (V2I) and Vehicle-to-IoT (V2IoT) ecosystem interactions. During this research, several field tests were conducted employing different types of communication modules, across three distinct environments: an open-field inter-urban road, a forest inter-urban road, and an urban road. The modules were evaluated based on the communication range, messaging rate, error rate, and geographical data from GNSS (Global Navigation Satellite System) coordinates, using point-to-point communication between a roadside unit (RSU) and a moving vehicle equipped with an onboard unit (OBU). The results demonstrate the usability of these technologies for integrating vehicles into both public infrastructure (for V2I services) and private IoT systems, highlighting their potential for scalable, cost-effective deployment in smart transportation systems. Full article
(This article belongs to the Topic Advances in Wireless and Mobile Networking)
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<p>Taxonomy of the benefits that integration of vehicles in IoT ecosystems can provide.</p>
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<p>Taxonomy of estimated benefits that result from the introduction of LPLR and low-cost communication technologies in a vehicular IoT environment.</p>
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<p>Typical elements and communication processes in a V2IoT system.</p>
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<p>The urban environment: (<b>a</b>) Representation on a map, with the red line marking the selected road and the red arrow indicating the position of the roadside unit (source: © OpenStreetMap contributors (“<a href="https://www.openstreetmap.org/copyright" target="_blank">https://www.openstreetmap.org/copyright</a>” (accessed on 29 September 2024))). Map data are available under the Open Database License (ODbL). (<b>b</b>) Photo.</p>
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<p>The open-field environment: (<b>a</b>) Representation on a map, with the red line marking the selected road and the red arrow indicating the position of the roadside unit (source: © OpenStreetMap contributors (“<a href="https://www.openstreetmap.org/copyright" target="_blank">https://www.openstreetmap.org/copyright</a>” (accessed on 29 September 2024))). Map data are available under the Open Database License (ODbL). (<b>b</b>) Photo.</p>
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<p>The forest environment: (<b>a</b>) Representation on a map, with the red line marking the selected road and the red arrow indicating the position of the roadside unit (source: © OpenStreetMap contributors (“<a href="https://www.openstreetmap.org/copyright" target="_blank">https://www.openstreetmap.org/copyright</a>” (accessed on 29 September 2024))). Map data are available under the Open Database License (ODbL). (<b>b</b>) Photo.</p>
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<p>Communication modules: (<b>a</b>) FSC-BT909C, (<b>b</b>) DX-BT27, (<b>c</b>) XBee Pro S2B, (<b>d</b>) XBee 3 Pro, (<b>e</b>) nRF24L01+PA+LNA, and (<b>f</b>) Adafruit RFM95W.</p>
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<p>Onboard module placement.</p>
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<p>Communication ranges for different road types.</p>
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<p>Effective communication rate for different environments.</p>
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<p>Average values of the CED and CLD for different road types.</p>
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<p>Maximum communication range vs. operational communication range for different road types.</p>
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16 pages, 1251 KiB  
Article
Effects of Dietary Protein and Lipid Levels on the Growth Performance and Serum Biochemical Indices of Juvenile Furong Crucian Carp
by Zhigang He, Xing Tian, Jinlong Li, Jiarong Guo, Xiaofei Cheng and Dongwu Wang
Fishes 2024, 9(11), 466; https://doi.org/10.3390/fishes9110466 - 16 Nov 2024
Viewed by 882
Abstract
The impact of dietary protein and lipid levels on the growth performance, feed utilization, and serum biochemical indices of Furong crucian carp was examined. Five hundred and forty carp (2.35 ± 0.08 g) were randomly assigned to nine groups and fed diets with [...] Read more.
The impact of dietary protein and lipid levels on the growth performance, feed utilization, and serum biochemical indices of Furong crucian carp was examined. Five hundred and forty carp (2.35 ± 0.08 g) were randomly assigned to nine groups and fed diets with three different protein levels (30.0, 35.0, and 40.0%) and three different lipid levels (4.0, 7.0, and 10.0%) for 60 days. The current findings revealed that the interaction effect between dietary lipid and protein levels exhibited significance for the final average weight (FAW), weight gain rate (WGR), specific growth rate (SGR), feed efficiency (FE), energy deposition rate (EDR), whole-fish energy, ash, and fat content (p < 0.05). Specifically, there was a significant reduction in FAW, WGR, and SGR with increasing dietary fat supplementation. Conversely, FE, EDR, and protein efficiency ratios were significantly decreased with increasing dietary protein levels (p < 0.05). Furthermore, serum albumin and globulin levels exhibited significant increases in response to dietary lipid inclusion (p < 0.05). The findings collectively indicate that Furong crucian carp fed a diet comprising 4% lipid and 30% protein exhibited the optimal growth and feed utilization. Conversely, excessive protein and lipid supplementation were detrimental to growth and resulted in the aggravation of metabolic disorders. Full article
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Graphical abstract
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<p>Potential regulatory patterns in Furong crucian carp. (<b>A</b>) Correlation analysis based on the Spearman coefficient was used for the interactions between the module eigengenes extracted from the matrix of the main and interaction effects of dietary lipid and protein inclusion levels and the secondary module eigenvectors extracted from the phenotype matrix. The presence of ‘*, **, ***’ indicated the significant difference level at 0.05, 0.01, and 0.001, respectively. The different size of diamond represented that the number of significant pairwise comparison in each row of the heat map. The arrow stated that at least one significant pairwise comparison was observed in the corresponding row; (<b>B</b>) structural equation model. The structural equation model explains as much variance as possible in the variables in the model while understanding the covariance between the variables.</p>
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15 pages, 7263 KiB  
Article
Reconstructing High Dynamic Range Image from a Single Low Dynamic Range Image Using Histogram Learning
by Huei-Yung Lin, Yi-Rung Lin, Wen-Chieh Lin and Chin-Chen Chang
Appl. Sci. 2024, 14(21), 9847; https://doi.org/10.3390/app14219847 - 28 Oct 2024
Viewed by 873
Abstract
High dynamic range imaging is an important field in computer vision. Compared with general low dynamic range (LDR) images, high dynamic range (HDR) images represent a larger luminance range, making the images closer to the real scene. In this paper, we propose an [...] Read more.
High dynamic range imaging is an important field in computer vision. Compared with general low dynamic range (LDR) images, high dynamic range (HDR) images represent a larger luminance range, making the images closer to the real scene. In this paper, we propose an approach for HDR image reconstruction from a single LDR image based on histogram learning. First, the dynamic range of an LDR image is expanded to an extended dynamic range (EDR) image. Then, histogram learning is established to predict the intensity distribution of an HDR image of the EDR image. Next, we use histogram matching to reallocate pixel intensities. The final HDR image is generated through regional adjustment using reinforcement learning. By decomposing low-frequency and high-frequency information, the proposed network can predict the lost high-frequency details while expanding the intensity ranges. We conduct the experiments based on HDR-Real and HDR-EYE datasets. The quantitative and qualitative evaluations have demonstrated the effectiveness of the proposed approach compared to the previous methods. Full article
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<p>Flowchart of the proposed two-stage approach.</p>
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<p>LDR2EDR process for dynamic range expansion. (<b>a</b>) original input histogram and its cumulative distribution; (<b>b</b>) histogram of the upsampled image and its cumulative distribution; (<b>c</b>) histogram of the downsampled image and its cumulative distribution.</p>
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<p>Histogram and its CDF of 12-bit EDR and HDR images. (<b>a</b>) histogram and its cumulative distribution of 12-bit EDR image; (<b>b</b>) histogram and its cumulative distribution of 12-bit HDR image.</p>
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<p>The cumulative histogram results. (<b>a</b>) Original output CDF and the corresponding histogram; (<b>b</b>) CDF and the corresponding histogram after curve smoothing; (<b>c</b>) CDF and the corresponding histogram after curve smoothing and monotonic constraint.</p>
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<p>The histogram matching results. (<b>a</b>) LDR image; (<b>b</b>) resulting HDR image; (<b>c</b>) ground-truth image.</p>
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<p>The resulting images of the proposed approach. The left column shows LDR images, the second column shows the resulting 12-bit HDR images of stage 1, the third column shows the resulting 16-bit HDR images of stage 2, and the right column illustrates the ground-truth HDR images. From the results, the proposed approach can reconstruct the desirable HDR images with high quality.</p>
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<p>The resulting images are compared with the input LDR images and ground truth HDR images in tone-mapped grayscale representations. The left column shows LDR images, the middle column shows our output HDR images, and the right column illustrates the ground-truth HDR images.</p>
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<p>The visual comparison of the proposed approach with the previous methods. (<b>a</b>) Input image, (<b>b</b>) HDRCNN, (<b>c</b>) DrTMO, (<b>d</b>) ExpandNet, (<b>e</b>) SingleHDR, and (<b>f</b>) the proposed approach.</p>
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<p>The visual comparison of the proposed approach with the previous methods. (<b>a</b>) Input image, (<b>b</b>) HDRCNN, (<b>c</b>) DrTMO, (<b>d</b>) ExpandNet, (<b>e</b>) SingleHDR, and (<b>f</b>) the proposed approach.</p>
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<p>The visual comparison of the proposed approach with the previous methods. (<b>a</b>) Input image, (<b>b</b>) HDRCNN, (<b>c</b>) DrTMO, (<b>d</b>) ExpandNet, (<b>e</b>) SingleHDR, and (<b>f</b>) the proposed approach.</p>
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<p>The visual comparison of the proposed approach with the previous methods. (<b>a</b>) Input image, (<b>b</b>) HDRCNN, (<b>c</b>) DrTMO, (<b>d</b>) ExpandNet, (<b>e</b>) SingleHDR, and (<b>f</b>) the proposed approach.</p>
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13 pages, 1314 KiB  
Article
Effect of Anthelmintic Treatment on the Agreement Between Real-Time Polymerase Chain Reaction (RT-PCR) and Kato–Katz Microscopic Technique in the Diagnosis of Soil-Transmitted Helminth Infections
by Paul Alvyn Nguema-Moure, Jean Claude Dejon-Agobé, Roméo-Aimé Laclong Lontchi, Gédéon Prince Manouana, Moustapha Nzamba Maloum, Brice Meulah, Danny Carrel Manfoumbi Mabicka, Marguerite Emmanuelle Nzame Ngome, Peter Gottfried Kremsner and Ayôla Akim Adegnika
Parasitologia 2024, 4(4), 345-357; https://doi.org/10.3390/parasitologia4040030 - 24 Oct 2024
Viewed by 928
Abstract
Soil-transmitted helminths (STHs), including Ascaris lumbricoides, hookworm species, and Trichuris trichiura, cause significant morbidity worldwide. For an effective proper control of their morbidity, accurate diagnosis method is needed. To this end, a polymerase chain reaction (PCR) method has been developed, but [...] Read more.
Soil-transmitted helminths (STHs), including Ascaris lumbricoides, hookworm species, and Trichuris trichiura, cause significant morbidity worldwide. For an effective proper control of their morbidity, accurate diagnosis method is needed. To this end, a polymerase chain reaction (PCR) method has been developed, but its use remains limited due to the high cost of its implementation, the resources required, and the lack of qualified technicians. The objective of the present analysis is to assess the agreement between the Kato–Katz microscopy method and quantitative real-time PCR (RT-PCR) in the diagnostic of STHs before and after treatment, to decipher the usefulness of either technique for evaluation of the treatment. Methods: Stool samples were collected before and after three- or six-weeks post-treatment from study participants and analyzed using Kato–Katz and RT-PCR methods for the diagnosis of STHs infections. The cure rate (CR) was estimated according to each diagnostic method. Agreement between CRs was tested using the Kappa statistical test. Results: Agreement between Kato–Katz and RT-PCR methods varied regarding the STH species targeted and was different after treatment compared with before treatment. At baseline, the two diagnostic methods showed a moderate agreement (0.45 < K < 0.5) for all STH species, whereas after treatment, concordance decreased slightly (0.11 ≤ K ≤ 0.14) for A. lumbricoides, remained moderate (0.5 ≤ K ≤ 0.53) for T. trichiura, and went from moderate to absent for hookworms. Conclusion: Our findings showed basically a moderate agreement between the Kato–Katz method and RT-PCR. There is a likely association with a moderate proportion of microscopy-positive cases. Reciprocally, a decrease in agreement after treatment was observed with low microscopy-positive cases after treatment, whereby RT-PCR was more likely to detect positive cases than microscopy. Therefore, the agreement is positively associated with an increasing in the number of samples testing positive. Full article
(This article belongs to the Special Issue The Molecular Epidemiology of Parasites)
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<p>Map of areas from Lambaréné and both rural areas where the study was conducted.</p>
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<p>Spearman correlation between helminth egg load determined by microscopy and RT-PCR Ct values. ρ = Spearman’s rho. 1, 2, and 3: correlation statement at baseline respectively for <span class="html-italic">A. lumbricoides</span>, <span class="html-italic">Trichuris trichuria</span>, and hookworms. 4, 5, and 6: Correlation statement at three weeks post-treatment (C1) respectively for <span class="html-italic">A. lumbricoides</span>, <span class="html-italic">T. trichuria</span>, and hookworms. 7, 8, and 9: Correlation statement at six weeks post-treatment (C2) respectively for <span class="html-italic">A. lumbricoides</span>, <span class="html-italic">T. trichuria</span>, and hookworms.</p>
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9 pages, 263 KiB  
Article
The Initial-Final Mass Relation from Carbon Stars in Open Clusters
by Carlos Abia, Inma Domínguez, Paola Marigo, Sergio Cristallo and Oscar Straniero
Galaxies 2024, 12(6), 67; https://doi.org/10.3390/galaxies12060067 - 23 Oct 2024
Viewed by 677
Abstract
Recently, Marigo et al, identified a kink in the initial-final mass relation around initial masses of Mini1.652.10M, based on Gaia DR2 and EDR3 data for white dwarfs in open clusters aged 1.5–2.5 Gyr. [...] Read more.
Recently, Marigo et al, identified a kink in the initial-final mass relation around initial masses of Mini1.652.10M, based on Gaia DR2 and EDR3 data for white dwarfs in open clusters aged 1.5–2.5 Gyr. Notably, the white dwarfs associated with this kink, all from NGC 7789, exhibit masses of ∼0.70–0.74 M, usually associated with stars of Mini 3–4 M. This kink in the Mini mass range coincides with the theoretically accepted solar metallicity lowest-mass stars evolving into carbon stars during the AGB phase. According to Marigo et al., these carbon stars likely experienced shallow third dredge-up events, resulting in low photospheric C/O ratios and, consequently, middle stellar winds. Under such conditions, the AGB phase is prolonged, allowing for further core mass growth beyond typical predictions. If this occurs, it might provoke other anomalies, such as a non-standard surface chemical composition. We have conducted a chemical analysis of several carbon stars belonging to open clusters within the above cluster ages. Our chemical analysis reveals that the carbon stars found within the kink exhibit C/O ratios only slightly above the unity and the typical chemical composition expected for carbon stars of near solar metallicity, partially validating the above theoretical predictions. We also show that this kink in the IMFR strongly depends on the method used to derived the distances (luminosity) of these carbon stars. Full article
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<p>The IFMR derived in this work (blue dots) for the stars studied (see text). Grey dots show the IFMR by [<a href="#B8-galaxies-12-00067" class="html-bibr">8</a>] derived on the basis of white dwarfs analysis. It is marked (red) the location of the star MSB 75, which belongs to the cluster NGC 7789 where some white dwarfs exceeding 0.7 M<sub>⊙</sub> have been found and suggest the existence of the kink ([<a href="#B11-galaxies-12-00067" class="html-bibr">11</a>,<a href="#B13-galaxies-12-00067" class="html-bibr">13</a>]). The virtual location of the C-stars IRAS 19582+2907 and Case 121 are also indicated (red); both stars apparently should have an initial mass M<sub><span class="html-italic">ini</span></sub> <math display="inline"><semantics> <mrow> <mo>&gt;</mo> <mn>4</mn> </mrow> </semantics></math> M<sub>⊙</sub> (see text).</p>
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14 pages, 4540 KiB  
Article
Short Peptides Protect Fibroblast-Derived Induced Neurons from Age-Related Changes
by Nina Kraskovskaya, Natalia Linkova, Elena Sakhenberg, Daria Krieger, Victoria Polyakova, Dmitrii Medvedev, Alexander Krasichkov, Mikhail Khotin and Galina Ryzhak
Int. J. Mol. Sci. 2024, 25(21), 11363; https://doi.org/10.3390/ijms252111363 - 22 Oct 2024
Viewed by 847
Abstract
Neurons become more vulnerable to stress factors with age, which leads to increased oxidative DNA damage, decreased activity of mitochondria and lysosomes, increased levels of p16, decreased LaminB1 proteins, and the depletion of the dendritic tree. These changes are exacerbated in vulnerable neuronal [...] Read more.
Neurons become more vulnerable to stress factors with age, which leads to increased oxidative DNA damage, decreased activity of mitochondria and lysosomes, increased levels of p16, decreased LaminB1 proteins, and the depletion of the dendritic tree. These changes are exacerbated in vulnerable neuronal populations during the development of neurodegenerative diseases. Glu-Asp-Arg (EDR) and Lys-Glu-Asp (KED), and Ala-Glu-Asp-Gly (AEDG) peptides have previously demonstrated neuroprotective effects in various models of Alzheimer’s disease. In this study, we investigated the influence of EDR, KED, and AEDG peptides on the aging of fibroblast-derived induced neurons. We used a new in vitro cellular model of human neuronal aging based on the transdifferentiation of aged dermal fibroblasts from elderly donors into induced cortical neurons. All peptides promote the arborization of the dendritic tree, increasing both the number of primary processes and the total length of dendrites. Tripeptides have no effect on the activity of mitochondria and lysosomes and the level of p16 protein in induced neurons. EDR peptide reduces oxidative DNA damage in induced neurons derived from elderly donor fibroblasts. Short peptides partially protect induced neurons from age-related changes and stimulate dendritogenesis in neurons. They can be recommended for use as neuroprotective agents. Full article
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<p>Microphotographs of dermal fibroblasts in the process of transdifferentiation in the neuronal direction (<b>A</b>) and after the application of short peptides (<b>B</b>). (<b>A</b>) Images illustrating changes in the morphology of induced neurons derived from dermal fibroblast lines of elderly donors at 0 days in vitro (DIV), 7 DIV, 14 DIV, and 28 DIV. Light microscopy, ×10. Scale bars correspond to 100 µm. (<b>B</b>) Images illustrating morphology of induced excitatory neurons at 38 DIV after application of short peptides AEDG, EDR, and KED. Light microscopy, ×10. Scale bars correspond to 100 µm. PID—post-infection day. EDR—Glu-Asp-Arg, KED—Lys-Glu-Asp, AEDG—Ala-Glu-Asp-Gl.</p>
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<p>Microphotographs illustrating the analysis of the 8-OHdG level in induced neurons after short peptide application. (<b>A</b>) Immunofluorescent staining with antibodies to 8-OHdG (secondary antibodies Alexa 488, green) and nuclei visualization with DAPI (blue) dye in the control cells and treated with short peptides AEDG, EDR, and KED in induced neurons derived from dermal fibroblast lines of elderly donors. Confocal microscopy, ×40. Scale bar 50 μm. (<b>B</b>) Histogram illustrating the 8-OHdG level calculation in induced neurons derived from dermal fibroblasts of elderly donors. Data are presented as mean ± SEM. * <span class="html-italic">p</span> &lt; 0.05—in comparison with the control group (C).</p>
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<p>Microphotographs illustrating the analysis of TMRM and lysotracker levels in induced neurons after the application of short peptides. (<b>A</b>) Live imaging staining with TMRM dye (red) and nuclei visualization with Hoechst dye (blue). Scanning confocal microscopy, ×10. Scale bar 200 um. (<b>B</b>) Histogram illustrating the mitochondrial activity detected by the TMRM in induced neurons derived from dermal fibroblast lines of elderly donors. Data are presented as mean ± SEM. (<b>C</b>) Live imaging staining with lysotracker dye (green) and nuclei visualization with Hoechst dye (blue). Scanning confocal microscopy, ×10. Scale bar 200 um (<b>D</b>) Histogram illustrating the mitochondrial activity detected by the lysotracker in induced neurons derived from dermal fibroblast lines of elderly donors. Data are presented as mean ± SEM.</p>
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<p>Microphotographs illustrating the analysis of p16 and laminB1 levels in induced neurons after application of short peptides. (<b>A</b>) Immunofluorescent staining of induced neurons with antibodies to laminB1 (secondary antibodies Alexa 488, green), p16 (secondary antibodies Alexa 555, red), and nuclei visualization with DAPI (blue) dye in the control cells and cells treated with short peptides AEDG, EDR, and KED. Scanning confocal microscopy, ×10. Scale bar 200 um. Histogram illustrating the level of p16 protein (<b>B</b>) and lamin B1 protein (<b>C</b>) expression in induced neurons derived from dermal fibroblast lines of elderly donors. Data are presented as mean ± SEM.</p>
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<p>Microphotographs illustrating the population of induced neurons (<b>A</b>) and morphology of individual neurons (<b>B</b>) after application of short peptide application. Immunofluorescent staining for the neuronal marker protein MAP2 (secondary antibodies Alexa 555, red) and nuclei visualization with DAPI (blue) dye of induced neurons derived from dermal fibroblast lines of elderly donors. Confocal microscopy, ×60 (<b>A</b>). Scale bar 50 μm.</p>
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<p>Histogram illustrating the number of primary processes (<b>A</b>), the number of branching points (<b>B</b>), the number of terminal processes (<b>C</b>), and the total length of dendrites (<b>D</b>) in the control and after application of peptide bioregulators in induced neurons obtained from dermal fibroblast lines and elderly donors. Data are presented as the mean ± SEM * <span class="html-italic">p</span> &lt; 0.05. ** <span class="html-italic">p</span> &lt; 0.01.</p>
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23 pages, 2789 KiB  
Article
Thermodynamic Comparative Analysis of Cascade Refrigeration System Pairing R744 with R404A, R448A and R449A with Internal Heat Exchanger: Part 2—Exergy Characteristics
by Min-Ju Jeon and Joon-Hyuk Lee
Energies 2024, 17(18), 4708; https://doi.org/10.3390/en17184708 - 21 Sep 2024
Viewed by 719
Abstract
The cascade refrigeration systems (CRS) used in hypermarkets and supermarkets, which are used by many people, have been employing R744 for the low-temperature cycle (LTC) and R404A for the high-temperature cycle (HTC) due to environmental and public safety issues. However, the use of [...] Read more.
The cascade refrigeration systems (CRS) used in hypermarkets and supermarkets, which are used by many people, have been employing R744 for the low-temperature cycle (LTC) and R404A for the high-temperature cycle (HTC) due to environmental and public safety issues. However, the use of R404A is limited due to its high GWP, and therefore research on alternative refrigerants is necessary. Nevertheless, there is no detailed study in the literature that compares and analyzes the three refrigerants for practical design by applying R744 for LTC and R404A, R448A, and R449A for HTC in CRS. Therefore, this study aims to provide data for the practical detailed design of an alternative system to R744/R404A CRS. Under standard conditions, we analyzed how the exergy destruction rate (EDR) and exergy efficiency (EE) of the system and the EDR of each component change when the important factors affecting CRS (degree of superheating (DSH), degree of subcooling (DSC), and internal heat exchanger (IHX) efficiency of HTC, DSH of LTC, condensation temperature (CT), evaporation temperature (ET), cascade evaporation temperature (CET), and temperature difference of CHX) are varied over a wide range. The main conclusions are as follows. (1) Under the given constant conditions, the smallest change in system EDR based on R448A is DSH of HTC (decreased by 0.07–0.1 kW), followed by IHX of HTC (decreased by 0.12–0.3 kW), DSH of LTC (increased by 0.19–0.25 kW), DSC of HTC (decreased by 0.59–0.69 kW), temperature difference of CHX (increased by 1.57–1.83 kW), CET (decreased and then increased by 0.67–4.43 kW), CT (increased by 1.49–3.9 kW), ET (decreased by 2.39–4.61 kW). (2) The highest change rate of system EE based on R448A is CET (increased and then decreased by 1.38–8.28%), followed by temperature difference of CHX (decreased by 2.96–3.16%), ET (increased and then decreased by 0.63–2.75%), DSC of HTC (increased by 1.26–1.34%), CT (increased and then decreased by 0.24–1.12%), IHX of HTC (increased by 0.11–1.02%), DSH of LTC (decreased by 0.35–0.49%), and DSH of HTC (increased by 0.14–0.19%). Full article
(This article belongs to the Section J: Thermal Management)
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<p>Conceptual diagram of the CRS pairing R744 with R404A, R448A and R449A applying IHX [<a href="#B30-energies-17-04708" class="html-bibr">30</a>].</p>
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<p>P-h diagram of a CRS pairing R744 with R404A, R448A and R449A with IHX [<a href="#B30-energies-17-04708" class="html-bibr">30</a>].</p>
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<p>T-s diagram of a CRS pairing R744 with R404A, R448A and R449A with IHX [<a href="#B30-energies-17-04708" class="html-bibr">30</a>].</p>
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<p>EDR, EE and COP of the CRS with respect to DSC in the HTC.</p>
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<p>EDR of each component with respect to DSC of the HTC.</p>
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<p>EDR, EE and COP of the CRS with respect to DSH in the HTC.</p>
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<p>EDR of each component with respect to DSH in the HTC.</p>
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<p>EDR, EE and COP of the CRS with respect DSH in the LTC.</p>
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<p>EDR of each component with respect to DSH in the LTC.</p>
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<p>EDR, EE and COP of the CRS with respect CT.</p>
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<p>EDR of each component with respect to CT.</p>
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<p>EDR, EE and COP of the CRS with respect ET.</p>
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<p>EDR of each component with respect to ET.</p>
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<p>EDR, EE and COP of the CRS with respect CET.</p>
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<p>EDR of each component with respect to CET.</p>
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<p>EDR, EE and COP of the CRS with respect temperature difference of CHX.</p>
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<p>EDR of each component with respect to temperature difference of CHX.</p>
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<p>EDR, EE and COP of the CRS with respect to IHX efficiency in the HTC.</p>
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<p>EDR of each component with respect to IHX efficiency in the HTC.</p>
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13 pages, 4434 KiB  
Article
Licorice Extract Isoliquiritigenin Protects Endothelial Function in Type 2 Diabetic Mice
by Lin Wang, Ruiwen Zhu, Chufeng He, Huixian Li, Qile Zhang, Yiu Ming Cheung, Fung Ping Leung and Wing Tak Wong
Nutrients 2024, 16(18), 3160; https://doi.org/10.3390/nu16183160 - 19 Sep 2024
Viewed by 1395
Abstract
Endothelial dysfunction occurs prior to atherosclerosis, which is an independent predictor of cardiovascular diseases (CVDs). Diabetes mellitus impairs endothelial function by triggering oxidative stress and inflammation in vascular tissues. Isoliquiritigenin (ISL), one of the major bioactive ingredients extracted from licorice, has been reported [...] Read more.
Endothelial dysfunction occurs prior to atherosclerosis, which is an independent predictor of cardiovascular diseases (CVDs). Diabetes mellitus impairs endothelial function by triggering oxidative stress and inflammation in vascular tissues. Isoliquiritigenin (ISL), one of the major bioactive ingredients extracted from licorice, has been reported to inhibit inflammation and oxidative stress. However, the therapeutic effects of ISL on ameliorating type 2 diabetes (T2D)-associated endothelial dysfunction remain unknown. In our animal study, db/db male mice were utilized as a model for T2D-associated endothelial dysfunction, while their counterpart, heterozygote db/m+ male mice, served as the control. Mouse brain microvascular endothelial cells (mBMECs) were used for in vitro experiments. Interleukin-1β (IL-1β) was used to induce endothelial cell dysfunction. ISL significantly reversed the impairment of endothelium-dependent relaxations (EDRs) in db/db mouse aortas. ISL treatment decreased ROS (reactive oxygen species) levels in db/db mice aortic sections and IL-1β-treated endothelial cells. Encouragingly, ISL attenuated the overexpression of pro-inflammatory factors MCP-1, TNF-α, and IL-6 in db/db mouse aortas and IL-1β-impaired endothelial cells. The NOX2 (NADPH oxidase 2) overexpression was inhibited by ISL treatment. Notably, ISL treatment restored the expression levels of IL-10, SOD1, Nrf2, and HO-1 in db/db mouse aortas and IL-1β-impaired endothelial cells. This study illustrates, for the first time, that ISL attenuates endothelial dysfunction in T2D mice, offering new insights into the pharmacological effects of ISL. Our findings demonstrate the potential of ISL as a promising therapeutic agent for the treatment of vascular diseases, paving the way for the further exploration of novel vascular therapies. Full article
(This article belongs to the Special Issue Healthy Diet to Prevent Cardiovascular Disease)
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Graphical abstract
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<p>Isoliquiritigenin (ISL) treatment reverses endothelial dysfunction in type 2 diabetes mouse aortas. (<b>A</b>) Experimental design of the animal study. (<b>B</b>) Representative tracings and (<b>C</b>) summarized results showing the beneficial effects of ISL treatment on impaired acetylcholine-induced endothelium-dependent relaxations (EDRs) in <span class="html-italic">db</span>/<span class="html-italic">db</span> mice. (<b>D</b>) Endothelium-independent relaxations in response to sodium nitroprusside (SNP) remain similar among different groups. (<b>E</b>,<b>F</b>) qPCR analysis demonstrates that ISL treatment downregulates mRNA expression levels of VCAM-1 (<b>E</b>) and ICAM-1 (<b>F</b>) in <span class="html-italic">db</span>/<span class="html-italic">db</span> mice. (<b>G</b>,<b>H</b>) Aortas isolated from <span class="html-italic">db</span>/<span class="html-italic">m</span><sup>+</sup> and <span class="html-italic">db</span>/<span class="html-italic">db</span> mice were treated with 5 μmol/L ISL for 16 h. (<b>G</b>) Summarized results showing the beneficial effects of ISL treatment on impaired acetylcholine-induced EDRs in <span class="html-italic">db</span>/<span class="html-italic">db</span> mice. (<b>H</b>) Endothelium-independent relaxations in response to SNP remained similar among different groups. Data represent means ± SEM of 6 mice. mRNA expressions were normalized with β-actin. <span class="html-italic">p</span> values were determined using two-way ANOVA for (<b>C</b>,<b>D</b>,<b>G</b>,<b>H</b>) and one-way ANOVA for (<b>E</b>,<b>F</b>). <sup>##</sup> <span class="html-italic">p</span> &lt; 0.0001 <span class="html-italic">db</span>/<span class="html-italic">db</span> vs. <span class="html-italic">db</span>/<span class="html-italic">m</span><sup>+</sup>, ** <span class="html-italic">p</span> &lt; 0.01 <span class="html-italic">db</span>/<span class="html-italic">db</span> + ISL vs. <span class="html-italic">db</span>/<span class="html-italic">db</span> (C,D,G,H). ** <span class="html-italic">p</span> &lt; 0.01 (<b>E</b>,<b>F</b>). ISL, isoliquiritigenin; OGTT, oral glucose tolerance test; qPCR, quantitative polymerase chain reaction; ROS, reactive oxygen species; ELISA, enzyme-linked immunosorbent assay; ACh, acetylcholine; SNP, sodium nitroprusside; VCAM-1, vascular cell adhesion molecule 1; and ICAM-1, intercellular adhesion molecule 1.</p>
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<p>Oral ISL treatment attenuates oxidative stress in <span class="html-italic">db</span>/<span class="html-italic">db</span> mouse aortas. <span class="html-italic">db</span>/<span class="html-italic">db</span> mice were treated with 20 mg/kg of ISL via oral gavage for 8 weeks. (<b>A</b>) Representative images and (<b>B</b>) summarized results of DHE staining demonstrate that ROS levels increase in <span class="html-italic">db</span>/<span class="html-italic">db</span> mouse aortas. ISL treatment decreases ROS levels in <span class="html-italic">db</span>/<span class="html-italic">db</span> mouse aortas. (<b>C</b>–<b>F</b>) qPCR analysis demonstrates that ISL treatment downregulates the mRNA expression level of NOX2 (<b>C</b>) in <span class="html-italic">db</span>/<span class="html-italic">db</span> mouse aortas. ISL upregulates mRNA expression levels of SOD1 (<b>D</b>), Nrf2 (<b>E</b>), and HO-1 (<b>F</b>) in <span class="html-italic">db</span>/<span class="html-italic">db</span> mouse aortas. Scale bar: 100 μm. Data represent the means ± SEM of 6 mice. mRNA expressions were normalized with β-actin. <span class="html-italic">p</span> values were determined using one-way ANOVA (<b>B</b>–<b>F</b>). ** <span class="html-italic">p</span> &lt; 0.01. ISL, isoliquiritigenin; DHE, dihydroethidium; NOX2, NADPH oxidase 2; SOD1, superoxide dismutase 1; Nrf2, nuclear factor erythroid-2 related factor 2; and HO-1, heme oxygenase-1.</p>
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<p>ISL suppresses oxidative stress in isolated <span class="html-italic">db</span>/<span class="html-italic">db</span> mouse aortas and IL-1β-treated endothelial cells. (<b>A</b>,<b>B</b>) Aortas isolated from <span class="html-italic">db</span>/<span class="html-italic">m</span><sup>+</sup> and <span class="html-italic">db</span>/<span class="html-italic">db</span> mice were treated with 5 μmol/L ISL for 16 h. (<b>A</b>) Representative images and (<b>B</b>) summarized results of DHE staining demonstrate that ISL decreases enhanced ROS levels in <span class="html-italic">db</span>/<span class="html-italic">db</span> mouse aortas. (<b>C</b>,<b>H</b>) Endothelial cells were treated with IL-1β (1 ng/mL) and/or ISL (5 μmol/L) for 16 h. (<b>C</b>) Representative images and (<b>D</b>) summarized results of DHE staining indicate that ISL suppresses heightened ROS levels in IL-1β-treated endothelial cells. (<b>E</b>–<b>H</b>) qPCR analysis demonstrates that ISL treatment downregulates mRNA expression levels of NOX2 (<b>E</b>) in IL-1β-treated endothelial cells. ISL restores SOD1 (<b>F</b>), Nrf2 (<b>G</b>), and HO-1 (<b>H</b>) downregulation in IL-1β-treated endothelial cells. Scale bar: 100 μm (<b>A</b>), 25 μm (<b>C</b>). Data represent the means ± SEM of 6 mice (<b>A</b>,<b>B</b>) or 5 experiments (<b>C</b>–<b>H</b>). mRNA expressions were normalized with β-actin. <span class="html-italic">p</span> values were determined using one-way ANOVA for (<b>B</b>–<b>H</b>). ** <span class="html-italic">p</span> &lt; 0.01. ISL, isoliquiritigenin; DHE, dihydroethidium; IL-1β, interleukin-1β; NOX2, NADPH oxidase 2; SOD1, superoxide dismutase 1; Nrf2, nuclear factor erythroid-2 related factor 2; and HO-1, heme oxygenase-1.</p>
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<p>ISL attenuates inflammation in <span class="html-italic">db</span>/<span class="html-italic">db</span> mouse aortas and IL-1β-treated endothelial cells. (<b>A</b>–<b>D</b>) <span class="html-italic">db</span>/<span class="html-italic">db</span> mice were treated with 20 mg/kg of ISL via oral gavage for 8 weeks. qPCR analysis demonstrates that ISL treatment downregulates mRNA expression levels of MCP-1 (<b>A</b>), TNF-α (<b>B</b>), and IL-6 (<b>C</b>) in <span class="html-italic">db</span>/<span class="html-italic">db</span> mouse aortas. ISL upregulates the mRNA expression of IL-10 (<b>D</b>) in <span class="html-italic">db</span>/<span class="html-italic">db</span> mouse aortas. (<b>E</b>–<b>H</b>) Endothelial cells were treated with IL-1β (1 ng/mL) and/or ISL (5 μmol/L) for 16 h. qPCR analysis demonstrates that ISL treatment downregulates mRNA expression levels of MCP-1 (<b>E</b>), TNF-α (<b>F</b>), and IL-6 (<b>G</b>) in IL-1β-treated endothelial cells. ISL upregulates the mRNA expression of IL-10 (<b>H</b>) in IL-1β-endothelial cells. mRNA expressions were normalized to β-actin. Data represent the means ± SEM of 6 mice (<b>A</b>–<b>D</b>) or 5 experiments (<b>E</b>–<b>H</b>). <span class="html-italic">p</span> values were determined using one-way ANOVA. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01. ISL, isoliquiritigenin; IL-1β, interleukin-1β; MCP-1, monocyte chemoattractant protein 1; TNF-α, tumor necrosis factor-α; IL-6, interleukin-6; and IL-10, interleukin-10.</p>
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25 pages, 1233 KiB  
Article
Human Papillomavirus Genotypes Distribution in High-Grade Cervical Lesions and Invasive Cervical Carcinoma in Women Living in Mauritania: Implications for Cervical Cancer Prevention and HPV Prophylactic Vaccination
by Mariem Salma Abdoudaim, Mohamed Val Mohamed Abdellahi, Nacer Dine Mohamed Baba, Ralph-Sydney Mboumba Bouassa, Mohamed Lemine Cheikh Brahim Ahmed and Laurent Bélec
Diagnostics 2024, 14(17), 1986; https://doi.org/10.3390/diagnostics14171986 - 8 Sep 2024
Cited by 1 | Viewed by 1403
Abstract
Cervical cancer related to high-risk human papillomavirus (HR-HPV) is the second female cancer in Mauritania (Northwest Sahelian Africa). We assessed the distribution of HPV genotypes in Mauritanian women with high-grade cervical intraepithelial neoplasia (CIN2/3) or invasive cervical cancer (ICC). A prospective study was [...] Read more.
Cervical cancer related to high-risk human papillomavirus (HR-HPV) is the second female cancer in Mauritania (Northwest Sahelian Africa). We assessed the distribution of HPV genotypes in Mauritanian women with high-grade cervical intraepithelial neoplasia (CIN2/3) or invasive cervical cancer (ICC). A prospective study was conducted in the Centre Hospitalier National, Nouakchott, Mauritania, to collect cervical biopsies among women suspected of CIN2/3 or cancer. HPV DNA detection and genotyping were carried out from formalin-fixed, paraffin-embedded biopsies using multiplex PCR (Human Papillomavirus Genotyping Real-Time PCR Kit, Bioperfectus Technologies Co., Taizhou, China). Fifty biopsies were included from women (mean age: 56.7 years) suffering from CIN2/3 (28.0%) and ICC (72.0%) which corresponded to 32 (64.0%) squamous cell carcinomas (SCC) and 4 (8.0%) adenocarcinomas (ADC). HPV DNA detection was successful in 47 (94.0%) samples. The most prevalent HR-HPV genotypes were HPV-45 (40.4%), HPV-16 (38.3%), HPV-39 and HPV-52 (23.4%), HPV-33 (17.0%), HPV-18 (14.9%), HPV-35 (4.2%), and HPV-56 (2.1%). The majority (93.6%) of HPV-positive biopsies contained at least one HPV type covered by the 9-valent Gardasil-9® vaccine, and 40.9% were infected by multiple vaccine HPV genotypes. To eradicate cervical cancer in Mauritania, prophylactic HPV vaccination must be combined with primary molecular screening of cervical HR-HPV infection. Full article
(This article belongs to the Section Diagnostic Microbiology and Infectious Disease)
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<p>Geographical map of Mauritania. Mauritania, officially the Islamic Republic of Mauritania, is a sovereign country in northwest Africa. It is bordered by the Atlantic Ocean to the West, western Sahara to the north and the northwest, Algeria to the northeast, Mali to the east and the southeast, and Senegal to the southwest. Mauritania is the 11th largest country in Africa, and 90% of its territory is situated in the Sahara Desert. Most of its population of 4.73 million inhabitants live in the temperate south of the country, with roughly one-third concentrated in the capital and largest city, Nouakchott, located on the Atlantic coast. All study participants were included in the Centre Hospitalier National in Nouakchott.</p>
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<p>Distribution of HPV types by histology. The samples were classified as high-grade cervical intraepithelial neoplasia (CIN2/3) or invasive cervical cancer, including adenocarcinoma (ADC) or squamous cell carcinoma (SCC). Nota bene: According to the manufacturer’s instructions and in accordance with the HPV classification nomenclature provided by the International Agency for Research on Cancer, the BMRT HPV Genotyping Real-Time PCR Kit distinguishes 21 HPV genotypes, including 14 genotypes (HPV-16, -18, -31, -33, -35, -39, -45, -51, -52, -56, -58, -59, -66, and -68), 3 LR-HPV types (HPV-6, -11, and -81), and 4 genotypes classified as possibly oncogenic (HPV-26, -53, -73, and -82).</p>
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<p>Distribution of HPV genotypes according to their inclusion in the 9-valent Gardasil-9<sup>®</sup> vaccine: Percentage of low-risk (LR), high-risk (HR), and possibly carcinogenic HPV genotypes in 47 cervical samples positive for HPV DNA by molecular biology according to their possible prevention by Gardasil-9<sup>®</sup> vaccine among adult women living in Mauritania. Nota bene: The 9-valent Gardasil-9<sup>®</sup> vaccine (Merck &amp; Co. Inc., Rahway, NJ, USA) targets the seven HR-HPV genotypes predominantly isolated in cervical cancer (HPV-16, -18, -31, -33, -45, -52, and -58) and two LR-HPV (HPV-6 and HPV-11).</p>
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<p>Percentage of low-risk (LR), high-risk (HR), and possibly carcinogenic HPV genotypes in 47 cervical biopsies samples from adult women living in Mauritania, positive for HPV DNA not targeted by the 9-valent Gardasil-9<sup>®</sup> vaccine by using the BMRT HPV Genotyping Real-Time PCR Kit. Nota bene: The 9-valent Gardasil-9<sup>®</sup> vaccine (Merck &amp; Co. Inc., Rahway, NJ, USA) targets the seven HR-HPV genotypes predominantly isolated in cervical cancer (HPV-16, -18, -31, -33, -45, -52, and -58) and two LR-HPV (HPV-6 and HPV-11).</p>
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17 pages, 4100 KiB  
Article
Buckling Capacity of Steel Spherical Shells According to B&M Procedure Compared to Selected Experimental Studies
by Paweł Błażejewski
Appl. Sci. 2024, 14(17), 7835; https://doi.org/10.3390/app14177835 - 4 Sep 2024
Viewed by 648
Abstract
This article compares the results of estimating the buckling capacity of steel spherical shells using simple engineering formulas with the results of selected experimental studies that have been conducted over the past 70 years. It is worth noting that these studies were conducted [...] Read more.
This article compares the results of estimating the buckling capacity of steel spherical shells using simple engineering formulas with the results of selected experimental studies that have been conducted over the past 70 years. It is worth noting that these studies were conducted by research centers from all over the world, and the tested coatings differed in material, manufacturing method, radius R, and thickness t. The test methods, procedures, and measurement tools were also different. The convergence of the results obtained between the proposed simple engineering approach, which is based on the provisions of EN-1993-1-6 and the recommendations in EDR5th, and the experimental studies is satisfactory, and in many cases the results obtained are almost identical. Therefore, it is possible to practically apply the developed algorithms for estimating the buckling capacity of steel spherical shells without worrying too much about the capacity reserve. Full article
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<p>View of testing fixture. According to [<a href="#B9-applsci-14-07835" class="html-bibr">9</a>].</p>
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<p>Comparison of the test results of Kaplan and Fung [<a href="#B9-applsci-14-07835" class="html-bibr">9</a>] with proposed B&amp;M buckling capacity curves.</p>
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<p>Schematic of the test apparatus. According to [<a href="#B10-applsci-14-07835" class="html-bibr">10</a>].</p>
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<p>Comparison of the test results of L. Seaman [<a href="#B10-applsci-14-07835" class="html-bibr">10</a>] with proposed B&amp;M buckling capacity curves.</p>
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<p>Sketches of models in pressure tanks. According to [<a href="#B11-applsci-14-07835" class="html-bibr">11</a>].</p>
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<p>Comparison of the test results of Krenzke and Kiernan [<a href="#B11-applsci-14-07835" class="html-bibr">11</a>] with proposed B&amp;M buckling capacity curves.</p>
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<p>Idealized geometry diagram of the tested spherical shells. According to [<a href="#B12-applsci-14-07835" class="html-bibr">12</a>].</p>
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<p>Comparison of the test results of J. Błachut [<a href="#B12-applsci-14-07835" class="html-bibr">12</a>] with proposed B&amp;M buckling capacity curves.</p>
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<p>View of the test pressure chamber. According to [<a href="#B14-applsci-14-07835" class="html-bibr">14</a>].</p>
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<p>Comparison of the test results of S. Kołodziej and J. Marcinowski [<a href="#B13-applsci-14-07835" class="html-bibr">13</a>] with proposed B&amp;M buckling capacity curves.</p>
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<p>Idealized geometry diagram of the tested spherical shell. According to [<a href="#B6-applsci-14-07835" class="html-bibr">6</a>].</p>
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<p>The form of buckling of the tested spherical caps SC1–SC6. According to [<a href="#B7-applsci-14-07835" class="html-bibr">7</a>].</p>
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<p>Comparison of the test results of J. Zhang et al. [<a href="#B7-applsci-14-07835" class="html-bibr">7</a>] with proposed B&amp;M buckling capacity curves.</p>
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<p>Welding line: (<b>a</b>) HS-1 and HS-4; (<b>b</b>) HS-2; (<b>c</b>) HS-3; (<b>d</b>) side view, photograph before painting; (<b>e</b>) HS-1; (<b>f</b>) HS-2; and before testing; (<b>g</b>) HS-3; (<b>h</b>) HS-4. According to [<a href="#B8-applsci-14-07835" class="html-bibr">8</a>].</p>
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<p>Schematic of the test stand and view of the two pressure chambers.</p>
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<p>Comparison of the test results of S. Cho et al. [<a href="#B8-applsci-14-07835" class="html-bibr">8</a>] with proposed B&amp;M buckling capacity curves.</p>
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17 pages, 2956 KiB  
Article
Experimental Investigation of R404A Indirect Refrigeration System Applied Internal Heat Exchanger: Part 2—Exergy Characteristics
by Min-Ju Jeon and Joon-Hyuk Lee
Energies 2024, 17(16), 4143; https://doi.org/10.3390/en17164143 - 20 Aug 2024
Viewed by 709
Abstract
Although the R404A indirect refrigeration system (IRS) with an internal heat exchanger (IHX) and R744 as the secondary fluid has potential applications in supermarkets and hypermarkets, the exergy characteristics of this IRS have not been extensively investigated. In this study, the factors affecting [...] Read more.
Although the R404A indirect refrigeration system (IRS) with an internal heat exchanger (IHX) and R744 as the secondary fluid has potential applications in supermarkets and hypermarkets, the exergy characteristics of this IRS have not been extensively investigated. In this study, the factors affecting the R744 exergy characteristics (degree of subcooling (DSB) and degree of superheating (DSP) of the R404A cycle, DSP of the R744 cycle, condensation temperature (CT) and cascade evaporation temperature (CET), and IHX efficiency) were experimentally evaluated to obtain basic data for the design of R404A IRS with R744 as the optimal secondary fluid. The main results can be summarized as follows: (1) Under given conditions, the smallest change in the system exergy destruction rate (EDR) according to the change in each parameter is the DSP of the R744 cycle (0.3–1%), followed by the DSB of the R404A cycle (6.1–8.8%), the IHX efficiency of the R404A cycle (3.8–14.3%), the DSP of the R404A cycle (11.7–15.9%), the CET (29.4–41.9%), and the CT (35–47%). (2) Also, in terms of the exergy efficiency of system (EES), the largest value was obtained for the DSP of the R404A cycle (2.4–12.7%), followed by the IHX efficiency of the R404A cycle (3–10.2%), the CET (2.2–8.7%), the CT (4–6.9%), the DSB of the R404A cycle (2.7–6.2%), and the DSP of the R744 cycle (0.04–1.2%). (3) In order to lower the system EDR, DSP, DSB, and IHX efficiency of the R404A cycle, the CET must be increased to the maximum, and to lower the DSP of the R744 cycle, the CT must be reduced to the minimum. Full article
(This article belongs to the Section J: Thermal Management)
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<p>Photograph of the test apparatus: the R404A IRS using R744 as the secondary fluid.</p>
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<p>Schematic of the test apparatus: the R404A IRS using R744 as the secondary fluid [<a href="#B25-energies-17-04143" class="html-bibr">25</a>].</p>
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<p>Conceptual diagram of the R404A IRS, using R744 as the secondary fluid.</p>
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<p>COP, EDR, and EES of the IRS according to the DSP of the R404A cycle.</p>
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<p>COP, EDR, and EES of the IRS according to the DSP of the R744 cycle.</p>
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<p>COP, EDR, and EES of the IRS according to the DSB of the R404A cycle.</p>
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<p>COP, EDR, and EES of the IRS with respect to the CT of the R404A cycle.</p>
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<p>COP, EDR, and EES of the IRS according to the CET.</p>
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<p>COP, EDR, and EES of the IRS according to the IHX efficiency of the R404A cycle.</p>
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<p>Performance analysis results according to the DSP of the R404A cycle in the IRS.</p>
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