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19 pages, 11551 KiB  
Article
Mechanical Performance of rPET Filament Obtained by Thermal Drawing for FFF Additive Manufacturing
by Pedro Pires, Martim Lima de Aguiar and André Costa Vieira
J. Manuf. Mater. Process. 2025, 9(1), 26; https://doi.org/10.3390/jmmp9010026 - 16 Jan 2025
Viewed by 86
Abstract
The growing production of plastic waste and its recycling, from a circular economy perspective, faces challenges in finding solutions that are easy to implement, cheap in labor and energy during recycling, and locally implementable to avoid transportation. This work developed and validated a [...] Read more.
The growing production of plastic waste and its recycling, from a circular economy perspective, faces challenges in finding solutions that are easy to implement, cheap in labor and energy during recycling, and locally implementable to avoid transportation. This work developed and validated a methodology to address these challenges. Designed for small-scale use at home or in schools following a Do It Yourself (DIY) approach, it transforms water bottles into plastic strips, which, after passing through an extruder nozzle, become filaments with a diameter of 1.75 mm. These can replace commercially available thermoplastic filaments. Specimens produced by additive manufacturing with recycled PET (rPET) and commercial PETG showed similar mechanical properties and can serve as alternatives to commercial PETG. PETG shows higher strength (30 MPa) compared to rPET (24 MPa), a slightly higher Young’s modulus of 1.44 GPa versus 1.43 GPa, and greater strain at failure with 0.03 mm/mm against 0.02 mm/mm, making it stiffer and more ductile. This simple and widely applicable local solution may absorb a considerable amount of bottle waste, offering an economical, sustainable alternative to commercial filaments. Full article
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<p>Plastic strip cutter.</p>
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<p>Machine CAD model and assembled components: (<b>a</b>) JGY370 motor; (<b>b</b>) PWM controller; (<b>c</b>) gear system; (<b>d</b>) Mean Well LRS-100-24 source; (<b>e</b>) W3230; (<b>f</b>) heating cartridge; (<b>g</b>) k-type thermocouple sensor; (<b>h</b>) AC power socket; (<b>i</b>) spool for storage of plastic strip; (<b>j</b>) extrusion nozzle (<b>k</b>) heating block.</p>
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<p>Preparation of the bottle: (<b>a</b>) before; (<b>b</b>) after.</p>
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<p>rPET filament ready to use.</p>
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<p>Printed temperature tower.</p>
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<p>Printed speed tower.</p>
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<p>Top layers: commercial filament (<b>a</b>) vs. rPET (<b>b</b>).</p>
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<p>Cross-section of rPET filament.</p>
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<p>Filament section area estimation in SolidWorks.</p>
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<p>Samples with different flow values.</p>
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<p>Test piece dimensions in millimeters.</p>
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<p>Printing orientations of the three different samples tested, adapted from [<a href="#B24-jmmp-09-00026" class="html-bibr">24</a>]. (<b>a</b>) Samples at 0° printing orientation; (<b>b</b>) samples at 90° printing orientation; (<b>c</b>) samples at 45° printing orientation.</p>
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<p>Shimadzu AGS-50 kN universal testing machine.</p>
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<p>Representative stress × strain graph of PETG and rPET at 45°.</p>
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<p>Stress × strain graph of PETG and rPET at 90°.</p>
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<p>Stress × strain graph of PETG and rPET at 0°.</p>
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<p>Stress × deformation graph of rPET from different brands.</p>
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<p>Stress × deformation graph of rPET and PET.</p>
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17 pages, 2428 KiB  
Article
The Criticality of the Digital Economy in Environmental Sustainability: Fresh Insights from a Wavelet-Based Quantile-on-Quantile Approach
by Xiaoqing Wong, Wenhao Kang, Jisu Kim, Yingying Xu and Ankang Wang
Sustainability 2025, 17(2), 622; https://doi.org/10.3390/su17020622 - 15 Jan 2025
Viewed by 460
Abstract
Achieving environmental sustainability has become an urgent priority in the era of rapid digital economic expansion, which presents both opportunities and challenges for environmental sustainable development. This study investigates the impact of digital economy (DIE) on environmental sustainability (ENS) through the dual dimensions [...] Read more.
Achieving environmental sustainability has become an urgent priority in the era of rapid digital economic expansion, which presents both opportunities and challenges for environmental sustainable development. This study investigates the impact of digital economy (DIE) on environmental sustainability (ENS) through the dual dimensions of digital industrialization (DII) and industrial digitalization (IND), employing the wavelet-based quantile-on-quantile regression method to capture both quantile dependencies and temporal variations. The results reveal that DIE positively impacts ENS in the long term, while its short-term effects are mixed, with positive effects at lower and higher quantiles but negative impacts at mid-range quantiles of [0.35–0.45] and [0.65–0.7]. Specifically, DII exerts a predominantly negative short-term effect on ENS due to the environmental costs of digital infrastructure expansion, but turns positive in the long term as digital industrialization matures, especially in [0.85–0.95]. IND, conversely, exerts a consistently positive impact on ENS in both short- and long-term scenarios, highlighting its role in enhancing industrial efficiency and reducing emissions. These results emphasize the need for targeted policies, including prioritizing industrial digitalization, developing green infrastructure, and adopting phased digital development strategies to maximize environmental benefits. Full article
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<p>Time series of DIE, DII, and IND with wavelet decomposition and ENS.</p>
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<p>The influence coefficients of DIE on ENS.</p>
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<p>The influence coefficients of DII on ENS.</p>
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<p>The influence coefficients of IND on ENS.</p>
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<p>Quantile regression (the solid black line) and QQR estimates (the dashed red line).</p>
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15 pages, 22427 KiB  
Article
Codesigning More-than-Human Ecosystems with Social and Environmental Systems: The Gamification of NetWall and BioDiveIn
by Marie Davidová, María Claudia Valverde Rojas and Hanane Behnam
Land 2025, 14(1), 165; https://doi.org/10.3390/land14010165 - 15 Jan 2025
Viewed by 390
Abstract
This study explores the integration of gamification into social and environmental systems to enhance urban biodiversity and foster the co-creation of ecosystems. It focuses on two key contributions: the development of tangible ecosystemic interventions, such as habitat extensions and edible landscapes, and the [...] Read more.
This study explores the integration of gamification into social and environmental systems to enhance urban biodiversity and foster the co-creation of ecosystems. It focuses on two key contributions: the development of tangible ecosystemic interventions, such as habitat extensions and edible landscapes, and the gamification of these interventions to engage communities. The interventions were codesigned using systems-oriented design methods, including gigamapping and prototyping, to produce scalable DIY solutions that empower communities to replicate these practices on their own. Additionally, urban games were created to incentivize participation by rewarding individuals for their contributions to biodiversity restoration. Full article
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<p>NetWall Intervention, Stuttgart-Germany (Photo: Behnam 2024).</p>
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<p>BioDiveIn Intervention, Stuttgart-Germany (Photo: Behnam 2023).</p>
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<p>NetWall gigamapping codesign workshop with stakeholders (Photo: Behnam 2023).</p>
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<p>COLife game gigamapping codesign workshops with stakeholders, highlighting the iterative feedback loop process and collaborative design approach (Photo: Behnam 2023–2024).</p>
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<p>NetWall final gigamap for exhibition purposes (COLife Studio 2024). This image showcases the material and layout of the final gigamap developed for the NetWall intervention, designed for exhibition purposes. For a detailed exploration of the content and connections represented in the gigamap, refer to the dataset: [<a href="#B7-land-14-00165" class="html-bibr">7</a>] Davidova et al. (2024), COLife_03—Gigamap and DIY (V1 ed.), DaRUS. <a href="https://doi.org/10.18419/darus-3986" target="_blank">https://doi.org/10.18419/darus-3986</a>.</p>
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<p>COLife final game gigamap for exhibition purposes (COLife Studio 2024). This figure presents the final gigamap used for the COLife game, illustrating the extent and structure of relationships captured during the design process. For detailed content, refer to the dataset: [<a href="#B12-land-14-00165" class="html-bibr">12</a>] Davidova et al. (2024), COLife_05: Gigamap and Game Introduction (V1 ed.), DaRUS. <a href="https://doi.org/10.18419/darus-4437" target="_blank">https://doi.org/10.18419/darus-4437</a>.</p>
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<p>NetWall synthesis map for exhibition purposes (COLife Studio 2024). This synthesis map for the NetWall intervention distills key elements from the gigamap to facilitate understanding during exhibitions. Readers seeking to explore the full map and its detailed content should refer to the dataset: [<a href="#B7-land-14-00165" class="html-bibr">7</a>] Davidova et al. (2024), COLife_03—Gigamap and DIY (V1 ed.), DaRUS. <a href="https://doi.org/10.18419/darus-3986" target="_blank">https://doi.org/10.18419/darus-3986</a>.</p>
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<p>NetWall DIY map for reproduction and exhibition purposes (COLife Studio 2024). This DIY map highlights the step-by-step process for reproducing the NetWall intervention, created for exhibition and community use. For a deeper understanding of the underlying content, refer to the dataset: [<a href="#B7-land-14-00165" class="html-bibr">7</a>] Davidova et al. (2024), COLife_02—Gigamap and Game Design (V1 ed.), DaRUS. <a href="https://doi.org/10.18419/darus-3985" target="_blank">https://doi.org/10.18419/darus-3985</a>.</p>
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<p>GoCOLife game design (COLife Summer 2023 Studio 2023). <a href="https://interacty.me/projects/d4d916f6b1a8609b" target="_blank">https://interacty.me/projects/d4d916f6b1a8609b</a> (accessed on 11 January 2025) [<a href="#B11-land-14-00165" class="html-bibr">11</a>] Davidová, M.; Behnam, H.; Valverde Rojas, M.C.; Guerriero, C.; Yeh, H.; Huang, J.; Köse, M. COL-ife_02—Gigamap and Game Design; University of Stuttgart: Stuttgart, Germany, 2024. <a href="https://doi.org/10.18419/darus-3985" target="_blank">https://doi.org/10.18419/darus-3985</a>.</p>
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<p>CoLife Web interface structure, retrieved from <a href="https://colifebiodiversity.wixsite.com/co-life-1" target="_blank">https://colifebiodiversity.wixsite.com/co-life-1</a> (accessed on 11 January 2025) (COLife Studio 2024). [<a href="#B12-land-14-00165" class="html-bibr">12</a>] Davidova, M.; Valverde Rojas, M.C.; Behnam, H.; Montserrat Castillo Cordova, A.; Çavuşoğlu, S.; Eyüboğlu, H.; Gado, N.; Grgurovic, N.; Sayyad, Z.; Skorniewska, K. COLife_05: Gigamap and Game Introduction; University of Stuttgart: Stuttgart, Germany, 2024. <a href="https://doi.org/10.18419/darus-4437" target="_blank">https://doi.org/10.18419/darus-4437</a>.</p>
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<p>Seed bombing at NetWall installation opening (Photo: Behnam 2024).</p>
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18 pages, 2329 KiB  
Article
Communication and Sensing: Wireless PHY-Layer Threats to Security and Privacy for IoT Systems and Possible Countermeasures
by Renato Lo Cigno, Francesco Gringoli, Stefania Bartoletti, Marco Cominelli, Lorenzo Ghiro and Samuele Zanini
Information 2025, 16(1), 31; https://doi.org/10.3390/info16010031 - 7 Jan 2025
Viewed by 315
Abstract
Recent advances in signal processing and AI-based inference enable the exploitation of wireless communication signals to collect information on devices, people, actions, and the environment in general, i.e., to perform Integrated Sensing And Communication (ISAC). This possibility offers exciting opportunities for Internet of [...] Read more.
Recent advances in signal processing and AI-based inference enable the exploitation of wireless communication signals to collect information on devices, people, actions, and the environment in general, i.e., to perform Integrated Sensing And Communication (ISAC). This possibility offers exciting opportunities for Internet of Things (IoT) systems, but it also introduces unprecedented threats to the security and privacy of data, devices, and systems. In fact, ISAC operates in the wireless PHY and Medium Access Control (MAC) layers, where it is impossible to protect information with standard encryption techniques or with any other purely digital methodologies. The goals of this paper are threefold. First, it analyzes the threats to security and privacy posed by ISAC and how they intertwine in the wireless PHY layer within the framework of IoT and distributed pervasive communication systems in general. Secondly, it presents and discusses possible countermeasures to protect users’ security and privacy. Thirdly, it introduces an architectural proposal, discussing the available choices and tradeoffs to implement such countermeasures, as well as solutions and protocols to preserve the potential benefits of ISAC while ensuring data protection and users’ privacy. The outcome and contribution of the paper is a systematic argumentation on wireless PHY-layer privacy and security threats and their relation with ISAC, framing the boundaries that research and innovation in this area should respect to avoid jeopardizing people’s rights. Full article
(This article belongs to the Special Issue Data Privacy Protection in the Internet of Things)
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<p>Visual illustration of some threats at the PHY layer in the context of 5G networks. (<b>a</b>) Jamming and eavesdropping attacks; (<b>b</b>) Spoofing, MITM, and wormhole attacks.</p>
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<p>People and objects directly affect the propagation of wireless signals. Encryption of the data payload cannot prevent an eavesdropper from monitoring the environment using the electromagnetic properties of the transmitted signals.</p>
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<p>General scenario for smart mobility: Legitimate devices receive normal communication signals between users and the infrastructure. An attacker may overhear normal signals from fixed transmitters (blue devices) or inject additional signals (orange devices).</p>
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<p>Obfuscation of an indoor passive attack. The legitimate AP transmitter randomly pre-distorts the signals to mimic ever-changing ambient propagation.</p>
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<p>Obfuscation of an indoor active attack with the use of an RIS. The attacker controls one or more transmitters and one or more receivers; thus, the only possibility of protection is with intelligent surfaces that reflect the incoming signal with pseudo-random, sub-symbol delays.</p>
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<p>De-obfuscation signaling (red arrows) exploiting the secure channel after the 802.11i 4WHS.</p>
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<p>De-obfuscation signaling (red arrows) to drive the RIS and the de-obfuscation at a legitimate sensing device.</p>
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15 pages, 1139 KiB  
Article
The Association Between the Dietary Inflammatory Index, Dietary Pattern, and Hypertension Among Residents in the Xinjiang Region
by Min Wang, Jiali Liao, Hao Wang, Lu Deng, Tingyu Zhang, Heng Guo, Xin Qian and Rulin Ma
Nutrients 2025, 17(1), 165; https://doi.org/10.3390/nu17010165 - 1 Jan 2025
Viewed by 877
Abstract
Background: Diet and inflammation are both associated with hypertension. We aimed to investigate the relationship between the dietary inflammation index (DII), dietary patterns, and the risk of hypertension among Xinjiang residents. Methods: A total of 930 residents aged 20–80 from Shihezi [...] Read more.
Background: Diet and inflammation are both associated with hypertension. We aimed to investigate the relationship between the dietary inflammation index (DII), dietary patterns, and the risk of hypertension among Xinjiang residents. Methods: A total of 930 residents aged 20–80 from Shihezi and Tumushuk were selected as participants using a stratified whole cluster random sampling method. General demographic information, dietary data, and physical examination results were collected from the participants and DII scores were calculated. Restricted cubic spline was used to analyze the dose–response relationship between the DII and the risk of hypertension. LASSO regression was used to screen dietary factors associated with hypertension. Factor analysis was used to extract dietary patterns. Finally, logistic regression modeling was used to analyze the association between the DII, dietary patterns, and the risk of hypertension. Results: The DII was linearly and positively associated with the risk of developing hypertension. Logistic regression analysis showed that the prevalence of hypertension was 2.23 (95% CI: 1.53, 3.23) and 3.29 (95% CI: 2.26, 4.79) in the T2 and T3 groups, respectively, compared with the T1 group. Riboflavin and folate were associated with the risk of hypertension. In the vegetable–egg dietary pattern, the risk of hypertension was reduced by 33%, 39%, and 37% in groups Q2, Q3, and Q4, respectively, compared with group Q1 (Q2: OR = 0.67, 95% CI: 0.45, 0.99; Q3: OR = 0.61, 95% CI: 0.41, 0.92; Q4: OR = 0.63, 95% CI: 0.42, 0.96). Conclusions: The higher the DII score, the higher the risk of hypertension among residents of Xinjiang. In addition, vegetable–egg dietary patterns can reduce the risk of hypertension. Therefore, local residents should be scientifically instructed to increase their intake of vegetables and eggs. Full article
(This article belongs to the Section Nutritional Immunology)
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<p>Flow chart of the study population.</p>
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<p>Restricted cubic spline curve of DII and the risk of hypertension.</p>
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<p>LASSO regression screening for key dietary factors associated with hypertension. (<b>a</b>) LASSO regression coefficient plots. (<b>b</b>) Cross-validation plots. LASSO regression, minimum absolute contraction, and selection operator.</p>
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<p>Development and validation of a predictive model for hypertension risk. (<b>a</b>) Column nomogram of key dietary factors associated with hypertension identified by LASSO regression. The green lines indicate the individual scores for each variable, and the blue lines indicate the risk of developing hypertension corresponding to the total score when the individual scores for both variables are added together. (<b>b</b>) ROC curves evaluating the predictive ability of the column-line graph model for hypertension. LASSO: least absolute shrinkage, and selection operator; ROC: receiver operator characteristic curve.</p>
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19 pages, 3029 KiB  
Article
Optimizing Velocity Field Measurement with 3D-Printed Particles and MATLAB: A Cost-Effective System for Flow Visualization
by José Juan Aliaga-Maraver, Ángel Antonio Rodríguez-Sevillano, María Jesús Casati-Calzada, Rafael Bardera-Mora, Estela Barroso-Barderas, Juan Carlos García-Matías, Alfonso Láinez-Muñiz and Davide Visentin
Aerospace 2025, 12(1), 11; https://doi.org/10.3390/aerospace12010011 - 27 Dec 2024
Viewed by 330
Abstract
This article aims to highlight the importance of including quantitative measurements when conducting flow visualization tests, such as those performed in towing tanks, within fluid mechanics analysis. It investigates the possibility of measuring velocity fields with an economically accessible technique compared to other [...] Read more.
This article aims to highlight the importance of including quantitative measurements when conducting flow visualization tests, such as those performed in towing tanks, within fluid mechanics analysis. It investigates the possibility of measuring velocity fields with an economically accessible technique compared to other techniques that require large financial investments, such as traditional PIV. The development of a MATLAB R2024b code based on image recognition and the use of 3D-printed tracer particles is proposed. Code workflow and how to make a correct selection of the processing parameters and its activity are explained and demonstrated on artificial images, generated by a computer, as well as real images, obtained in a 2D-test in the tank, achieving an accuracy, in absolute values, of 95%. However, the proposed velocimetry system currently has one important limitation, the impossibility of distinguishing between particles in different planes, which limits the study to two-dimensional tests. Then, the opportunity to include this technique in the study of more complex tests requires further investigation. Full article
(This article belongs to the Special Issue Droplet Impact for Airfoil Performance)
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<p>Button panel for the selection of processing parameters.</p>
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<p>Example of 15-pixel particle detection setting: (<b>a</b>) maximum radius 5, minimum radius 5, threshold 0. (<b>b</b>) maximum radius 5, minimum radius 5, threshold 0.3. (<b>c</b>) maximum radius 15, minimum radius 15, threshold 0.3.</p>
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<p>(<b>a</b>) Candidate pixel placed in a real circle (solid circle) next to the classical voting pattern (dashed circle). (<b>b</b>) Different voting patterns coincide at the center of the real circle. Extracted from [<a href="#B19-aerospace-12-00011" class="html-bibr">19</a>].</p>
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<p>Process summary.</p>
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<p>(<b>a</b>) 3D printing of particles. ABS material. (<b>b</b>) Zenith view from a section of the tank’s bottom including the drain and suspended particles in a laminar sheet of water.</p>
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<p>Computing time as a function of: (<b>a</b>) particle size; (<b>b</b>) number of particles; (<b>c</b>) number of images. Reference experiment: 9 images with 36 particles of 8 pixels. The computation times in (<b>a</b>) correspond to the identification time on a single image.</p>
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<p>Number of errors (false positives, blue; undetected particles, orange) as a function of varying the value of different processing parameters: (<b>a</b>) edge threshold, (<b>b</b>) range of radii, (<b>c</b>) search size and (<b>d</b>) filter applied to the image. Optimal parameters for the example: threshold 0.3, range 0, size 15, no filter.</p>
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<p>Comparison of results obtained from computer simulation and processing with the proposed code of synthetic images extracted: (<b>a</b>,<b>b</b>) trajectories, (<b>c</b>,<b>d</b>) velocity field (total magnitude) and (<b>e</b>,<b>f</b>) velocity field (distinction between x and y components).</p>
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<p>(<b>a</b>) Delaunay triangulation used to discretize the fluid domain. (<b>b</b>) Checking the estimation of vortex centers.</p>
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<p>Comparison of the velocity values obtained for each particle at their last position in the computer simulation (<b>a</b>) and after analyzing images 9 and 10 with the proposed code (<b>b</b>).</p>
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<p>Distribution of the absolute error occurred in the processing of synthetic images.</p>
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<p>Sources of error in particle center determination.</p>
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<p>Particle deposition time for different infill densities. ABS material.</p>
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<p>Results obtained after image processing of the tank emptying experiment: (<b>a</b>) velocity field (total magnitude), (<b>b</b>) velocity field (distinction between x and y components), (<b>c</b>) trajectories and (<b>d</b>) Delaunay triangulation.</p>
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<p>Error in tracking the trajectory of a particle due to its proximity to another particle.</p>
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13 pages, 2287 KiB  
Article
Development of an Easy-To-Use Microfluidic System to Assess Dynamic Exposure to Mycotoxins in 3D Culture Models: Evaluation of Ochratoxin A and Patulin Cytotoxicity
by Veronica Zingales, Caterina Piunti, Sara Micheli, Elisa Cimetta and María-José Ruiz
Foods 2024, 13(24), 4167; https://doi.org/10.3390/foods13244167 - 23 Dec 2024
Viewed by 565
Abstract
Mycotoxins are among the most concerning natural toxic food contaminants. Over the years, significant efforts have been made to characterize the risk associated with their exposure. However, assessing their toxicity has so far been elusive due to the lack of adequate models that [...] Read more.
Mycotoxins are among the most concerning natural toxic food contaminants. Over the years, significant efforts have been made to characterize the risk associated with their exposure. However, assessing their toxicity has so far been elusive due to the lack of adequate models that closely mimic the physiological conditions of human cells in vivo. Here, we present the SpheroFlow Device (SFD), an efficient microfluidic platform designed, manufactured, and validated to evaluate mycotoxin-induced cytotoxicity under dynamic and continuous exposure in 3D culture settings. In the present study, we integrated human neuroblastoma SH-SY5Y spheroids into the SFD to assess the acute toxicity induced by the mycotoxins ochratoxin A (OTA) and patulin (PAT). The developed system enabled qualitative and quantitative measurements of concentration–response relationships and provided accurate control over the culture microenvironment. Our findings show that by enhancing 3D culture model by applying dynamic flow, SH-SY5Y spheroids exhibited different sensitivities to OTA and PAT compared to conventional static SH-SY5Y spheroids, confirming the critical role of culture models in mycotoxin toxicity assessment. This is the first study assessing the neurotoxicity of OTA and PAT on 3D neuroblastoma spheroids considering the contribution of fluid flow. Full article
(This article belongs to the Special Issue Advances in the Monitoring and Analysis of Foodborne Pathogens)
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<p>SpheroFlow Device (SFD) design, fabrication, and validation. (<b>A</b>) Device design and mold fabrication: (<b>A-a</b>) The device was designed using AutoCAD<sup>®</sup>. Dimensions in mm. (<b>A-b</b>) Detail of the master mold produced via photolithography. (<b>B</b>) Microfluidic device assembly: (<b>B-a</b>) Microfluidic device in its final configuration and its individual parts: (<b>B-b</b>) upper layer, bottom layer sealed to a glass slide by plasma treatment (<b>B-c</b>) PDMS plugs. (<b>C</b>) Representative image of the fluid dynamic validation with flowing color tracers.</p>
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<p>Biological validation of the SFD. (<b>A</b>) Reconstruction of the entire length of the device including the inlet, culture wells, and outlet regions after 24 h of perfusion. Arrows indicate the spheroids. Scale bar: 1000 µm. (<b>B</b>) Cell viability (LIVE/DEAD assay) evaluated after 24 h of flow. From left to right: representative brightfield and fluorescent images of SH-SY5Y spheroids stained with Hoechst (nuclei, in blue), Calcein-AM (live cells, in green), and propidium iodide (dead cells, in red). Scale bar 1000 µm. Images were obtained using the fluorescence microscope EVOS™ FL at 4× magnification (Invitrogen, Carlsbad, CA, USA).</p>
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<p>Concentration–response relationship between OTA toxicity in SH-SY5Y spheroids integrated into the SFD. SH-SY5Y spheroids were exposed to increasing concentrations of OTA for 24 h at a flow rate of 1 µL/min. Cell viability was assessed via (<b>A</b>) LIVE/DEAD assay and (<b>B</b>) MTT assay. (<b>A</b>) Representative fluorescent images of spheroids stained with Hoechst (nuclei, in blue), Calcein-AM (live cells, in green) and propidium iodide (dead cells, in red). Images were obtained using a confocal fluorescence microscope (ZEISS LSM 800 Airyscan, Zeiss Microscopy, Germany), acquiring a z-stack of 10 slices for each spheroid to capture the information derived from the three-dimensionality of the structure. Images were processed using the orthogonal projection method. Scale bar: 50 µm (objective 20×). (<b>B</b>) Concentration–response curve from MTT data. Data are expressed as mean ± SEM of three independent experiments (<span class="html-italic">n</span> = 3). (*) <span class="html-italic">p</span> ≤ 0.05 indicates a significant difference compared to the control. Triton-X was used as a positive control.</p>
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<p>Concentration–response relationship of PAT toxicity in SH-SY5Y spheroids integrated into the SFD. SH-SY5Y spheroids were exposed to increasing concentrations of PAT for 24 h at a flow rate of 1 µL/min. Cell viability was assessed via (<b>A</b>) LIVE/DEAD assay and (<b>B</b>) MTT assay. (<b>A</b>) Representative fluorescent images of spheroids stained with Hoechst (nuclei, in blue), Calcein-AM (live cells, in green) and propidium iodide (dead cells, in red). Images were obtained using a confocal fluorescence microscope (ZEISS LSM 800 Airyscan, Zeiss Microscopy, Germany), acquiring a z-stack of 10 slices for each spheroid to capture the information derived from the three-dimensionality of the structure. Images were processed using the orthogonal projection method. Scale bar 100 µm (objective 10×). Panel (<b>B</b>) Concentration–response curve from MTT data. Data are expressed as mean ± SEM of three independent experiments (<span class="html-italic">n</span> = 3). (*) <span class="html-italic">p</span> ≤ 0.05 indicates a significant difference compared to the control. Triton-X was used as a positive control.</p>
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14 pages, 6442 KiB  
Article
Soil Water Status Monitoring System with Proximal Low-Cost Sensors and LoRa Technology for Smart Water Irrigation in Woody Crops
by Jorge Dafonte, Miguel Ángel González, Enrique Comesaña, María Teresa Teijeiro and Javier J. Cancela
Sensors 2024, 24(24), 8104; https://doi.org/10.3390/s24248104 - 19 Dec 2024
Viewed by 549
Abstract
Weather and soil water dictate farm operations such as irrigation scheduling. Low-cost and open-source agricultural monitoring stations are an emerging alternative to commercially available monitoring stations because they are often built from components using open-source, do-it-yourself (DIY) platforms and technologies. For irrigation management [...] Read more.
Weather and soil water dictate farm operations such as irrigation scheduling. Low-cost and open-source agricultural monitoring stations are an emerging alternative to commercially available monitoring stations because they are often built from components using open-source, do-it-yourself (DIY) platforms and technologies. For irrigation management in an experimental vineyard located in Quiroga (Lugo, Spain), we faced the challenge of installing a low-cost environmental and soil parameter monitoring station composed of several nodes measuring air temperature and relative humidity, soil temperature, soil matric potential, and soil water content. Commercial solutions were either too expensive or did not meet our needs. This challenge led us to design the low-cost sensor system that fulfilled our requirements. This node is based on the ESP32 chip, and communication between the nodes and the gateway is carried out by LoRa technology. The gateway is also based on the ESP32 chip. The gateway uploads the data to an FTP server using a Wi-Fi connection with a 4G router while simultaneously storing the data on a memory card. The programming of the code for the nodes and the gateway is performed using the Arduino IDE. The equipment developed is proven to be effective and for managing vineyard irrigation based on the built-in sensors, with replicable results. It is, however, essential to calibrate the capacitive sensors for measuring soil water content in each soil type in order to enhance their ability to produce reliable results. In addition, the limits marking the beginning and end of irrigation tasks must be adjusted to local conditions and according to the producer’s specific vineyard objectives. Full article
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<p>Experimental site location.</p>
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<p>Field node architecture of wireless sensor network.</p>
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<p>Schematic of circuit node.</p>
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<p>Flowchart of node programming (<b>left</b>) and flowchart of gateway programming (<b>right</b>).</p>
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<p>Node with board, connections, and microcontroller (<b>left</b>) and node installed in field (<b>right</b>).</p>
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<p>Daily average data of the output of the analog digital converter of the two capacitive sensors of soil water content (blue line capacitive sensor 1 and yellow line capacitive sensor 2) of node 1 and daily precipitation (blue bars).</p>
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<p>The 10 min data of matric potential in kPa at node 1 and 2 and daily precipitation (blue bars).</p>
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<p>A calibration curve of the voltage output of the capacitive sensor against the soil water content estimated with TDR for vineyard soil, raw data points (dots) and fitted model (dashed line).</p>
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<p>Measurements of Teros 12 sensors and DFRobot SEN0308 sensors in sand after saturation.</p>
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<p>DFRobot SEN0308 sensor measurements in sand after saturation and soil temperature. Red circles show the influence of soil temperature on the output of SEN0308.</p>
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19 pages, 349 KiB  
Article
Ethical Framework to Assess and Quantify the Trustworthiness of Artificial Intelligence Techniques: Application Case in Remote Sensing
by Marina Paolanti, Simona Tiribelli, Benedetta Giovanola, Adriano Mancini, Emanuele Frontoni and Roberto Pierdicca
Remote Sens. 2024, 16(23), 4529; https://doi.org/10.3390/rs16234529 - 3 Dec 2024
Cited by 1 | Viewed by 696
Abstract
In the rapidly evolving field of remote sensing, Deep Learning (DL) techniques have become pivotal in interpreting and processing complex datasets. However, the increasing reliance on these algorithms necessitates a robust ethical framework to evaluate their trustworthiness. This paper introduces a comprehensive ethical [...] Read more.
In the rapidly evolving field of remote sensing, Deep Learning (DL) techniques have become pivotal in interpreting and processing complex datasets. However, the increasing reliance on these algorithms necessitates a robust ethical framework to evaluate their trustworthiness. This paper introduces a comprehensive ethical framework designed to assess and quantify the trustworthiness of DL techniques in the context of remote sensing. We first define trustworthiness in DL as a multidimensional construct encompassing accuracy, reliability, transparency and explainability, fairness, and accountability. Our framework then operationalizes these dimensions through a set of quantifiable metrics, allowing for the systematic evaluation of DL models. To illustrate the applicability of our framework, we selected an existing case study in remote sensing, wherein we apply our ethical assessment to a DL model used for classification. Our results demonstrate the model’s performance across different trustworthiness metrics, highlighting areas for ethical improvement. This paper not only contributes a novel framework for ethical analysis in the field of DL, but also provides a practical tool for developers and practitioners in remote sensing to ensure the responsible deployment of DL technologies. Through a dual approach that combines top-down international standards with bottom-up, context-specific considerations, our framework serves as a practical tool for ensuring responsible AI applications in remote sensing. Its application through a case study highlights its potential to influence policy-making and guide ethical AI development in this domain. Full article
(This article belongs to the Special Issue Deep Learning Innovations in Remote Sensing)
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<p>Ethical evaluation process for remote sensing methodologies. The approach starts from the selection of the remote sensing methodology, applying the ethical evaluation framework with defined metrics (please refer to <a href="#sec3dot5-remotesensing-16-04529" class="html-sec">Section 3.5</a>), and concluding with the analysis and quantification of ethical compliance scores.</p>
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13 pages, 480 KiB  
Article
A Black Sticky Rice-Derived Functional Ingredient Improves Anxiety, Depression, and Stress Perception in Adult Volunteers
by Pattamaporn Natthee, Jintanaporn Wattanathorn, Wipawee Thukham-mee, Pongsatorn Paholpak, Poonsri Rangseekajee, Nawanant Piyavhatkul, Suphayanakorn Wattanathorn, Supaporn Muchimapura and Terdthai Tong-Un
Foods 2024, 13(23), 3884; https://doi.org/10.3390/foods13233884 - 1 Dec 2024
Viewed by 768
Abstract
We hypothesized that consumption of a diet containing the functional ingredient from black sticky rice, which is rich in anthocyanin, over a five-day period would improve anxiety, depression, and stress perception in adult volunteers based on the benefits of this compound. In this [...] Read more.
We hypothesized that consumption of a diet containing the functional ingredient from black sticky rice, which is rich in anthocyanin, over a five-day period would improve anxiety, depression, and stress perception in adult volunteers based on the benefits of this compound. In this study, a total of 46 male and female adult volunteers with mild and moderate stress level were assigned to groups consuming a breakfast meal containing an anthocyanin-enriched functional ingredient at doses of 2 and 4 g per day for 5 days. The volunteers consumed three meals with a low DII but high DAQ-S, and the total calories consumed during the study period was 2000 kcal/day. Mental well-being, including depression, anxiety, and stress, together with AChE, MAO, Nrf2, 8OHdG, MDA, and the density of Lactobacillus and Bifidobacterium spp., were assessed at baseline and at the end of the study. Safety parameters were also examined. A diet containing both doses of the anthocyanin-enriched functional ingredient with a low DII but high DAQ-S was found to improve anxiety, depression, and stress, with changes in 8-OHdG and IL-6 levels. No other changes and toxicity-related parameters were observed. Our results show that the novel functional ingredient can improve anxiety, depression, and stress perception partly by decreasing oxidative stress and inflammation; however, randomized, double-blind, placebo-controlled studies with a larger sample size should be performed to confirm this benefit. Full article
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<p>Schematic diagram illustrating the study protocol.</p>
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15 pages, 2051 KiB  
Article
The Impact of the Dietary Inflammatory Index, Fasting Blood Glucose, and Smoking Status on the Incidence and Survival of Pancreatic Cancer: A Retrospective Case–Control Study and a Prospective Study
by Ga Hyun Lee, Yeon Hee Kim, Sang Myung Woo, Woo Jin Lee, Sung-Sik Han, Sang-Jae Park, Sherry Price, Penias Tembo, James R. Hébert and Mi Kyung Kim
Nutrients 2024, 16(22), 3941; https://doi.org/10.3390/nu16223941 - 19 Nov 2024
Viewed by 936
Abstract
Background: Pancreatic cancer (PC), a highly malignant cancer with a poor diagnosis, may be influenced by diet-related inflammation. This study examined the association between dietary inflammatory index (DII) scores and the incidence and prognosis of PC in Korea. Methods: A total of 55 [...] Read more.
Background: Pancreatic cancer (PC), a highly malignant cancer with a poor diagnosis, may be influenced by diet-related inflammation. This study examined the association between dietary inflammatory index (DII) scores and the incidence and prognosis of PC in Korea. Methods: A total of 55 patients with PC were matched with 280 healthy controls (HCs) by age and sex. We also analyzed the combined effects of DII scores and fasting blood glucose (FBG) levels or smoking status on the risk of PC and performed a survival analysis using the Cox proportional hazards method. Results: The DII scores were higher in the patients with PC than those in HCs (odds ratio [OR] = 3.36, confidence interval [CI] = 1.16–9.73, p = 0.03), and the effect was larger in women (OR = 6.13, CI = 1.11–33.82, p = 0.04). A high DII score was jointly associated with FBG ≥ 126 mg/dL in raising PC risk [OR = 32.5, relative excess risk due to interaction/synergy (RERI/S) index = 24.2/4.34, p-interaction = 0.04], indicating a multiplicative interaction. A high DII score combined with ex/current smoker status increased PC risk through an additive interaction (RERI/S = 1.01/1.54, p-interaction = 0.76). However, DII scores did not influence disease-free survival. Conclusions: The consumption of an anti-inflammatory diet, coupled with maintaining normal FBG levels and abstaining from smoking, may help reduce the risk of PC by mitigating pancreatic inflammation. Full article
(This article belongs to the Special Issue Dietary Patterns and Cancer: Risks and Survival Outcomes)
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<p>The comparison of the DII and E-DII score for 55 PC cases and 280 HCs, stratified by sex. (<b>A</b>) DII scores between the groups; (<b>B</b>) DII scores between the groups for males; (<b>C</b>) DII scores between the groups for females; (<b>D</b>) E-DII scores between the groups; (<b>E</b>) E-DII scores between the groups for males; (<b>F</b>) E-DII scores between the groups for females. The data are presented as boxplots. <span class="html-italic">p</span>-values were calculated using the Mann–Whitney <span class="html-italic">U</span> test for DII and E-DII scores across all three groups. Abbreviations: DII, dietary inflammatory index; E-DII, energy-adjusted dietary inflammatory index; PC, pancreatic cancer; HC, healthy control.</p>
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<p>Multivariable logistic regression analyses of the three levels of DII scores for PC risk by sex. DII was adjusted for sex, age, BMI, smoking, and energy intake. DII was divided into three ranges, low, medium, and high DII, from the lowest to the highest. ORs and 95% CIs were evaluated to determine the relationship between the three levels of DII scores and PC risk using a multivariable logistic regression analysis for PCs and HCs. (<b>A</b>) ORs and 95% CIs among the three levels of DII; (<b>B</b>) ORs and 95% CIs among the three levels of DII for males; (<b>C</b>) ORs and 95% CIs among the three levels of DII for females. * <span class="html-italic">p</span> &lt; 0.05. The <span class="html-italic">p</span>-values for trends were computed by treating the values as continuous variables, evaluating the continuous scale, and allocating median values to each quantile. Abbreviations: OR, odds ratio; CI, confidence interval; Ref, reference value; DII, dietary inflammatory index; PC, pancreatic cancer; HC, healthy control.</p>
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<p>The adjusted Cox proportional hazards model for 5-year DFS, 5-year OS, and 5-year RFS of patients with PC by the DII score. The prognosis of the 55 PC cases was analyzed to assess DFS, OS, and RFS using the Cox proportional hazards model. This model was adjusted for sex, age, BMI, and energy intake. The DII score was divided into two groups: low DII and high DII. Survival curves based on DII scores were analyzed among 55 PCs for (<b>A</b>) 5-year DFS, (<b>B</b>) 5-year OS, (<b>C</b>) 5-year RFS, (<b>D</b>) 5-year DFS for males, (<b>E</b>) 5-year OS for males, (<b>F</b>) 5-year RFS for males, (<b>G</b>) 5-year DFS for females, (<b>H</b>) 5-year OS for females, and (<b>I</b>) 5-year RFS for females. Abbreviations: DFS, disease-free survival; OS, overall survival; RFS, recurrence-free survival; PC, pancreatic cancer; DII, dietary inflammatory index.</p>
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13 pages, 16117 KiB  
Article
A Stride Toward Wine Yield Estimation from Images: Metrological Validation of Grape Berry Number, Radius, and Volume Estimation
by Bernardo Lanza, Davide Botturi, Alessandro Gnutti, Matteo Lancini, Cristina Nuzzi and Simone Pasinetti
Sensors 2024, 24(22), 7305; https://doi.org/10.3390/s24227305 - 15 Nov 2024
Viewed by 587
Abstract
Yield estimation is a key point theme for precision agriculture, especially for small fruits and in-field scenarios. This paper focuses on the metrological validation of a novel deep-learning model that robustly estimates both the number and the radii of grape berries in vineyards [...] Read more.
Yield estimation is a key point theme for precision agriculture, especially for small fruits and in-field scenarios. This paper focuses on the metrological validation of a novel deep-learning model that robustly estimates both the number and the radii of grape berries in vineyards using color images, allowing the computation of the visible (and total) volume of grape clusters, which is necessary to reach the ultimate goal of estimating yield production. The proposed algorithm is validated by analyzing its performance on a custom dataset. The number of berries, their mean radius, and the grape cluster volume are converted to millimeters and compared to reference values obtained through manual measurements. The validation experiment also analyzes the uncertainties of the parameters. Results show that the algorithm can reliably estimate the number (MPE=5%, σ=6%) and the radius of the visible portion of the grape clusters (MPE=0.8%, σ=7%). Instead, the volume estimated in px3 results in a MPE=0.4% with σ=21%, thus the corresponding volume in mm3 is affected by high uncertainty. This analysis highlighted that half of the total uncertainty on the volume is due to the camera–object distance d and parameter R used to take into account the proportion of visible grapes with respect to the total grapes in the grape cluster. This issue is mostly due to the absence of a reliable depth measure between the camera and the grapes, which could be overcome by using depth sensors in combination with color images. Despite being preliminary, the results prove that the model and the metrological analysis are a remarkable advancement toward a reliable approach for directly estimating yield from 2D pictures in the field. Full article
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<p>Scheme of the inference process. The image is elaborated by the custom neural network and two probability density maps are returned as output. Pixel densities are summed to compute the estimate of the number of berries <math display="inline"><semantics> <mover accent="true"> <mi>N</mi> <mo stretchy="false">˜</mo> </mover> </semantics></math> and their average size <math display="inline"><semantics> <msub> <mover accent="true"> <mi>r</mi> <mo stretchy="false">˜</mo> </mover> <mi>mean</mi> </msub> </semantics></math>.</p>
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<p>Image examples taken from the Embrapa WGISD dataset [<a href="#B14-sensors-24-07305" class="html-bibr">14</a>].</p>
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<p>(<b>a</b>–<b>c</b>) Image examples of the test dataset along with manual annotations overlaid.</p>
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<p>Boxplot of the relative error <math display="inline"><semantics> <mrow> <mi>R</mi> <msub> <mi>E</mi> <mi>k</mi> </msub> </mrow> </semantics></math> in % computed for the number of visible berries, the value of the average radius, and the visible volume of the bunch depicted in the test images.</p>
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<p>Total bunch volume error <math display="inline"><semantics> <msub> <mi>E</mi> <mi>k</mi> </msub> </semantics></math> (difference between estimated volume <math display="inline"><semantics> <msub> <mover accent="true"> <mi>V</mi> <mo stretchy="false">˜</mo> </mover> <mrow> <mi>b</mi> <mo>,</mo> <mi>m</mi> <mi>m</mi> </mrow> </msub> </semantics></math> and nominal volume <math display="inline"><semantics> <msub> <mi>V</mi> <mrow> <mi>b</mi> <mo>,</mo> <mi>m</mi> <mi>m</mi> </mrow> </msub> </semantics></math>) for each image <span class="html-italic">k</span> in the test dataset, coupled with the corresponding uncertainty. The shaded red area corresponds to the <math display="inline"><semantics> <mrow> <mn>95</mn> <mo>%</mo> </mrow> </semantics></math> confidence interval of the ground truth.</p>
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20 pages, 609 KiB  
Article
Diet Quality and Dietary Intake in Breast Cancer Survivors Suffering from Chronic Pain: An Explorative Case-Control Study
by Sevilay Tümkaya Yılmaz, Ömer Elma, Jo Nijs, Peter Clarys, Iris Coppieters, Tom Deliens, Patrick Calders, Eline Naert and Anneleen Malfliet
Nutrients 2024, 16(22), 3844; https://doi.org/10.3390/nu16223844 - 9 Nov 2024
Viewed by 1446
Abstract
Background/Objectives: Dietary factors may significantly influence pain management in cancer survivors. However, a substantial gap exists regarding the relationship between nutrition and chronic pain in this population. This study examined differences in diet quality and dietary intake between breast cancer survivors (BCS) experiencing [...] Read more.
Background/Objectives: Dietary factors may significantly influence pain management in cancer survivors. However, a substantial gap exists regarding the relationship between nutrition and chronic pain in this population. This study examined differences in diet quality and dietary intake between breast cancer survivors (BCS) experiencing chronic pain and healthy controls (HC). It also aimed to understand the associations between dietary elements and pain-related outcomes within the BCS group. Methods: A case-control study was conducted with 12 BCS experiencing chronic pain and 12 HC (ages 18–65). Data collection included body composition, experimental pain assessments, pain-related questionnaires, and a 3-day food diary to calculate diet quality using the Healthy Eating Index-2015 (HEI-2015) and Dietary Inflammatory Index (DII). Statistical analyses evaluated group differences and associations between dietary factors and pain within the BCS group. Results: There were no significant differences in HEI-2015 scores between BCS and HC, but BCS had a significantly lower DII score (p = 0.041), indicating a more anti-inflammatory diet. BCS also showed higher intake of omega-3, vitamins B6, B12, A, D, and magnesium (p < 0.05). While total diet quality scores did not correlate with pain outcomes, several HEI-2015 and DII components, such as dairy, sodium, protein, vitamin C, and vitamin D, showed moderate positive or negative correlations with pain measures. Conclusions: Despite no overall differences in diet quality, BCS with chronic pain consumed more anti-inflammatory nutrients than HC. Complex correlations between specific dietary components and pain outcomes emphasise the need for further research to explore these links for chronic pain management in BCS. Full article
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<p>Flow of the study. * Test side changes were separated by a 30-s rest period within the measurement.</p>
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18 pages, 2655 KiB  
Article
Advanced Image Preprocessing and Integrated Modeling for UAV Plant Image Classification
by Girma Tariku, Isabella Ghiglieno, Anna Simonetto, Fulvio Gentilin, Stefano Armiraglio, Gianni Gilioli and Ivan Serina
Drones 2024, 8(11), 645; https://doi.org/10.3390/drones8110645 - 6 Nov 2024
Viewed by 1191
Abstract
The automatic identification of plant species using unmanned aerial vehicles (UAVs) is a valuable tool for ecological research. However, challenges such as reduced spatial resolution due to high-altitude operations, image degradation from camera optics and sensor limitations, and information loss caused by terrain [...] Read more.
The automatic identification of plant species using unmanned aerial vehicles (UAVs) is a valuable tool for ecological research. However, challenges such as reduced spatial resolution due to high-altitude operations, image degradation from camera optics and sensor limitations, and information loss caused by terrain shadows hinder the accurate classification of plant species from UAV imagery. This study addresses these issues by proposing a novel image preprocessing pipeline and evaluating its impact on model performance. Our approach improves image quality through a multi-step pipeline that includes Enhanced Super-Resolution Generative Adversarial Networks (ESRGAN) for resolution enhancement, Contrast-Limited Adaptive Histogram Equalization (CLAHE) for contrast improvement, and white balance adjustments for accurate color representation. These preprocessing steps ensure high-quality input data, leading to better model performance. For feature extraction and classification, we employ a pre-trained VGG-16 deep convolutional neural network, followed by machine learning classifiers, including Support Vector Machine (SVM), random forest (RF), and Extreme Gradient Boosting (XGBoost). This hybrid approach, combining deep learning for feature extraction with machine learning for classification, not only enhances classification accuracy but also reduces computational resource requirements compared to relying solely on deep learning models. Notably, the VGG-16 + SVM model achieved an outstanding accuracy of 97.88% on a dataset preprocessed with ESRGAN and white balance adjustments, with a precision of 97.9%, a recall of 97.8%, and an F1 score of 0.978. Through a comprehensive comparative study, we demonstrate that the proposed framework, utilizing VGG-16 for feature extraction, SVM for classification, and preprocessed images with ESRGAN and white balance adjustments, achieves superior performance in plant species identification from UAV imagery. Full article
(This article belongs to the Section Drones in Ecology)
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<p>Overall working process of the proposed approach.</p>
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<p>Sample single Ailanthus altissima plant image in the four-image dataset.</p>
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<p>VGG-16 architecture map.</p>
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<p>Accuracy of the four plant datasets using a combined VGG+16 feature extractor and machine learning classifiers SVM, random forest, XGBoost, and VGG-16 classifier.</p>
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<p>Confusion matrices comparing the performance of three machine learning models Support Vector Machine (SVM), random forest (RF), XGBoost, and VGG16 classifier across four datasets: (1) base dataset, (2) Base + ESRGAN, (3) Base + ESRGAN + CLAHE, and (4) Base + ESRGAN + WB. Each row corresponds to a different dataset, and each column represents the classification results from one of the models. The matrices illustrate the number of correct and incorrect classifications for various classes, with darker blue shades indicating higher values, highlighting the models’ performance across different data preprocessing techniques.</p>
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17 pages, 24201 KiB  
Article
An Echo State Network-Based Light Framework for Online Anomaly Detection: An Approach to Using AI at the Edge
by Andrea Bonci, Renat Kermenov, Lorenzo Longarini, Sauro Longhi, Geremia Pompei, Mariorosario Prist and Carlo Verdini
Machines 2024, 12(10), 743; https://doi.org/10.3390/machines12100743 - 21 Oct 2024
Viewed by 860
Abstract
Production efficiency is used to determine the best conditions for manufacturing goods at the lowest possible unit cost. When achieved, production efficiency leads to increased revenues for the manufacturer, enhanced employee safety, and a satisfied customer base. Production efficiency not only measures the [...] Read more.
Production efficiency is used to determine the best conditions for manufacturing goods at the lowest possible unit cost. When achieved, production efficiency leads to increased revenues for the manufacturer, enhanced employee safety, and a satisfied customer base. Production efficiency not only measures the amount of resources that are needed for production but also considers the productivity levels and the state of the production lines. In this context, online anomaly detection (AD) is an important tool for maintaining the reliability of the production ecosystem. With advancements in artificial intelligence and the growing significance of identifying and mitigating anomalies across different fields, approaches based on artificial neural networks facilitate the recognition of intricate types of anomalies by taking into account both temporal and contextual attributes. In this paper, a lightweight framework based on the Echo State Network (ESN) model running at the edge is introduced for online AD. Compared to other AD methods, such as Long Short-Term Memory (LSTM), it achieves superior precision, accuracy, and recall metrics while reducing training time, CO2 emissions, and the need for high computational resources. The preliminary evaluation of the proposed solution was conducted using a low-resource computing device at the edge of the real production machine through an Industrial Internet of Things (IIoT) smart meter module. The machine used to test the proposed solution was provided by the Italian company SIFIM Srl, which manufactures filter mats for industrial kitchens. Experimental results demonstrate the feasibility of developing an AD method that achieves high accuracy, with the ESN-based framework reaching 85% compared to 80.88% for the LSTM-based model. Furthermore, this method requires minimal hardware resources, with a training time of 9.5 s compared to 2.100 s for the other model. Full article
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<p>ESN diagram.</p>
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<p>LSTM diagram.</p>
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<p>Architecture for the computation of the standard deviation of the error in the training set.</p>
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<p>Framework architecture for the inference phase.</p>
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<p>Architecture diagram.</p>
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<p>Production machine and production layout.</p>
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<p>Cloud dashboard—CO<sub>2</sub> production.</p>
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<p>Standard deviation of the error of both the ESN and LSTM model-based approaches.</p>
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<p>Comparison between real time series and time-series prediction (ESN-based model on the left and LSTM-based model on the right).</p>
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<p>Accuracy for each epoch (LSTM-based and ESN-based methods).</p>
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