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22 pages, 4665 KiB  
Article
Enhancing Forest Structural Parameter Extraction in the Greater Hinggan Mountains: Utilizing Airborne LiDAR and Species-Specific Tree Height–Diameter at Breast Height Models
by Shaoyi Chen, Wei Chen, Xiangnan Sun and Yuanjun Dang
Forests 2025, 16(3), 457; https://doi.org/10.3390/f16030457 - 4 Mar 2025
Abstract
Forests, being the largest and most intricate terrestrial ecosystems, play an indispensable role in sustaining ecological balance. To effectively monitor forest productivity, it is imperative to accurately extract structural parameters such as the tree height and diameter at breast height (DBH). Airborne LiDAR [...] Read more.
Forests, being the largest and most intricate terrestrial ecosystems, play an indispensable role in sustaining ecological balance. To effectively monitor forest productivity, it is imperative to accurately extract structural parameters such as the tree height and diameter at breast height (DBH). Airborne LiDAR technology, which possesses the capability to penetrate canopies, has demonstrated remarkable efficacy in extracting these forest structural parameters. However, current research rarely models different tree species separately, particularly lacking comparative evaluations of tree height-DBH models for diverse tree species. In this study, we chose sample plots within the Bila River basin, nestled in the Greater Hinggan Mountains of the Inner Mongolia Autonomous Region, as the research area. Utilizing both airborne LiDAR and field survey data, individual tree positions and heights were extracted based on the canopy height model (CHM) and normalized point cloud (NPC). Six tree height-DBH models were selected for fitting and validation, tailored to the dominant tree species within the sample plots. The results revealed that the CHM-based method achieved a lower RMSE of 1.97 m, compared to 2.27 m with the NPC-based method. Both methods exhibited a commendable performance in plots with lower average tree heights. However, the NPC-based method showed a more pronounced deficiency in capturing individual tree information. The precision of grid interpolation and the point cloud density emerged as pivotal factors influencing the accuracy of both methods. Among the six tree height-DBH models, a multiexponential model demonstrated a superior performance for both oak and ”birch–poplar” trees, with R2 values of 0.479 and 0.341, respectively. This study furnishes a scientific foundation for extracting forest structural parameters in boreal forest ecosystems. Full article
(This article belongs to the Section Forest Inventory, Modeling and Remote Sensing)
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<p>Study area and the field surveyed plots in Oroqen Autonomous Banner, HulunBuir City, Inner Mongolia Autonomous Region of China.</p>
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<p>Tree height-DBH correlation plots for all sample trees (<b>a</b>), oak trees (<b>b</b>) and “birch–poplar” trees (<b>c</b>).</p>
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<p>The final results of DEM, DSM and CHM in the study area.</p>
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<p>Relative height normalization of airborne LiDAR point cloud data.</p>
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<p>Individual tree positions and tree height extraction using focal statistics based on CHM (taking plot 443 as an example). (<b>a</b>) is the initial CHM raster, (<b>b</b>) shows the focal statistics result, (<b>c</b>) shows the raster subtracting the focal statistics raster from the CHM, (<b>d</b>) shows the raster that assigned the CHM values from (<b>a</b>) to the tree tops in (<b>c</b>), (<b>e</b>) shows the raster after removing outliers and (<b>f</b>) shows the extracted individual tree positions as vector data.</p>
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<p>Individual tree positions extraction based on NPC (taking plot 443 as an example), the green triangles represent individual tree positions.</p>
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<p>Scatter plots of tree height and diameter for oak trees. Each subplot corresponds to a different model (M1–M6). The dark blue lines indicate observed trends, with shaded areas denoting confidence intervals.</p>
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<p>Scatter plots of tree height and diameter for “birch–poplar” trees. Each subplot corresponds to a different model (M1–M6). The dark blue lines indicate observed trends, with shaded areas denoting confidence intervals.</p>
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<p>Scatter plots of average tree height extracted from CHM and NPC versus field survey data.</p>
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18 pages, 3039 KiB  
Article
Exploring the Relationship Between Growth Strain and Growth Traits in Eucalyptus cloeziana at Different Age Stages
by Ying Huang, Jianzhong Wang, Yuan Pan, Haibo Zeng, Yunlin Fu and Penglian Wei
Sustainability 2025, 17(5), 2229; https://doi.org/10.3390/su17052229 - 4 Mar 2025
Abstract
The harvesting period is determined by forest maturity. However, there are few studies on the continuity of assessing cultivation duration based on both growth and wood quality, especially for Eucalyptus plantations. This study measures growth traits, such as the diameter at breast height [...] Read more.
The harvesting period is determined by forest maturity. However, there are few studies on the continuity of assessing cultivation duration based on both growth and wood quality, especially for Eucalyptus plantations. This study measures growth traits, such as the diameter at breast height (DBH), oblateness, and other characteristics, as well as wood properties like density and crystallinity, and axial surface growth strain levels at four age stages (6, 10, 22, and 34 years) of Eucalyptus cloeziana (E. cloeziana). By analyzing these factors, particularly the changes in growth strain throughout the tree’s development, the study aims to determine the optimal cultivation period for using E. cloeziana as solid wood. The survey revealed a two-stage pattern in the annual change rate of DBH, tree height, and oblateness: a decrease from 6 to 22 years followed by an increase from 22 to 34 years. In E. cloeziana, heartwood percentage and density rapidly declined during the first 6–10 years, then stabilized between 10 and 34 years. This suggested differential rates of growth and maturation. By analyzing the growth strain, it was observed that the growth strain of E. cloeziana exhibited an initial increase followed by a subsequent decrease with age. It reached its peak at 22 years and then gradually declined. Remarkably, at 34 years, the growth strain was even lower than that of 10-year-old E. cloeziana, measuring only 2148 με. This reduction in growth strain is advantageous for minimizing defects such as brittle core formation, cracking, and warping during harvesting. In practical cultivation aimed at solid wood utilization, harvesting can be conducted between 22 and 34 years based on management strategies to reduce operating costs. However, with close-to-nature management practices and sufficient financial resources, extending the cultivation period to 34 years or beyond may result in superior wood quality. We aim to achieve the sustainable utilization of resources, foster the long-term development of the wood processing and solid wood utilization industries, and guide the entire sector towards the goal of sustainable development. Full article
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<p>Growth strain measurement of <span class="html-italic">Eucalyptus cloeziana</span> (<span class="html-italic">E. cloeziana</span>).</p>
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<p>(<b>a</b>–<b>c</b>) show the changes in diameter at breast height (DBH, tree height, and oblateness at different age stages. (<b>d</b>) is the annual rate of change in DBH, tree height, and oblateness in four ages; ‘*’ indicates that DBH, tree height, and oblateness were significantly different at different age stages (<span class="html-italic">p</span> &lt; 0.05); ‘**’ indicates that DBH, tree height, and oblateness showed extremely significant differences at different ages (<span class="html-italic">p</span> &lt; 0.01).</p>
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<p>(<b>a</b>–<b>c</b>) show the changes in heartwood percentage, greenwood density, and basic density of <span class="html-italic">E. cloeziana</span> at different ages. (<b>d</b>) is the annual rate of change in heartwood percentage and density in four age stages; ‘*’ indicated that DBH, tree height, and oblateness were significantly different at different age stages (<span class="html-italic">p</span> &lt; 0.05); ‘**’ indicated that DBH, tree height, and oblateness showed extremely significant differences at different ages (<span class="html-italic">p</span> &lt; 0.01).</p>
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<p>(<b>a</b>) Growth strain analysis of <span class="html-italic">E. cloeziana</span> at different ages. (<b>b</b>) Distribution frequency diagram of total growth strain.</p>
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<p>Correlation between growth traits, physical properties, and growth strain of <span class="html-italic">E. cloeziana</span>; ‘*’ indicates that there is a significant correlation between different indicators and growth strain (<span class="html-italic">p</span> &lt; 0.05); ‘**’ indicates that there was an extremely significant correlation between difference indexes and growth strain (<span class="html-italic">p</span> &lt; 0.01). ‘***’ indicates that there was an extremely significant correlation between difference indexes and growth strain (<span class="html-italic">p</span> &lt; 0.001).</p>
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<p>X-ray diffraction pattern of <span class="html-italic">E. cloeziana</span>.</p>
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<p>Fourier transform infrared spectroscopy of wood cell wall of <span class="html-italic">E. cloeziana</span>.</p>
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24 pages, 6358 KiB  
Article
Improving Total Carbon Storage Estimation Using Multi-Source Remote Sensing
by Huoyan Zhou, Wenjun Liu, Hans J. De Boeck, Yufeng Ma and Zhiming Zhang
Forests 2025, 16(3), 453; https://doi.org/10.3390/f16030453 - 3 Mar 2025
Viewed by 114
Abstract
Accurate estimations of forest total carbon storage are essential for understanding ecosystem functioning and improving forest management. This study investigates how multi-source remote sensing data can be used to provide accurate estimations of diameter at breast height (DBH) at the plot level, enhancing [...] Read more.
Accurate estimations of forest total carbon storage are essential for understanding ecosystem functioning and improving forest management. This study investigates how multi-source remote sensing data can be used to provide accurate estimations of diameter at breast height (DBH) at the plot level, enhancing biomass estimations across 39.41 × 104 km2. The study is focused on Yunnan Province, China, which is characterized by complex terrain and diverse vegetation. Using ground-based survey data from hundreds of plots for model calibration and validation, the methodology combines multi-source remote sensing data, machine learning algorithms, and statistical analysis to develop models for estimating DBH distribution at regional scales. Decision tree showed the best overall performance. The model effectiveness improved when stratified by climatic zones, highlighting the importance of environmental context. Traditional methods based on the kNDVI index had a mean squared error (MSE) of 2575 t/ha and an R2 value of 0.69. In contrast, combining model-estimated DBH values with remote sensing data resulted in a substantially lower MSE of 212 t/ha and a significantly improved R2 value of 0.97. The results demonstrate that incorporating DBH not only reduced prediction errors but also improved the model’s ability to explain biomass variability. In addition, climatic region classification further increased model accuracy, suggesting that future efforts should consider environmental zoning. Our analyses indicate that water availability during cool and dry periods in this monsoon-influenced region was especially critical in influencing DBH across different subtropical zones. In summary, the study integrates DBH and high-resolution remote sensing data with advanced algorithms for accurate biomass estimation. The findings suggest that this approach can support regional forest management and contribute to research on carbon balance and ecosystem assessment. Full article
(This article belongs to the Section Forest Inventory, Modeling and Remote Sensing)
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<p>Yunnan Province in China and the plot of the study area.</p>
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<p>Technical route.</p>
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<p>The driving factors of DBH in plateau temperature region.</p>
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<p>The driving factors of DBH in south subtropical humid region.</p>
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<p>The driving factors of DBH in edge of humid region.</p>
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<p>Accuracy of model with kNDVI.</p>
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<p>Accuracy of the model without DBH.</p>
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<p>Accuracy of model with DBH.</p>
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<p>DBH (Plot scale) of Yunnan Province. Note: The DBH is the level of the plot, the size of each plot is 30 × 30 m, and the displayed value is the sum of DBH of all trees in the plot.</p>
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<p>Feature importance of DBH in Yunnan Province.</p>
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<p>Predicts total carbon storage of Yunnan Province: (<b>a</b>) current, (<b>b</b>) 2040–2060 SSP126, and (<b>c</b>) 2040–2060 SSP245.</p>
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<p>Change rate of total carbon storage in Yunnan Province. (<b>a</b>) ROC for SSP126(2040–2060) to current and (<b>b</b>) ROC for SSP245 (2040–2060) to current.</p>
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22 pages, 27425 KiB  
Article
Semiautomatic Diameter-at-Breast-Height Extraction from Structure-from-Motion-Based Point Clouds Using a Low-Cost Fisheye Lens
by Mustafa Zeybek
Forests 2025, 16(3), 439; https://doi.org/10.3390/f16030439 - 28 Feb 2025
Viewed by 304
Abstract
The diameter at breast height (DBH) is a fundamental index used to characterize trees and establish forest inventories. The conventional method of measuring the DBH involves using steel tape meters, rope, and calipers. Alternatively, this study has shown that it can be calculated [...] Read more.
The diameter at breast height (DBH) is a fundamental index used to characterize trees and establish forest inventories. The conventional method of measuring the DBH involves using steel tape meters, rope, and calipers. Alternatively, this study has shown that it can be calculated automatically using image-based algorithms, thus reducing time and effort while remaining cost-effective. The method consists of three main steps: image acquisition using a fisheye lens, 3D point cloud generation using structure-from-motion (SfM)-based image processing, and improved DBH estimation. The results indicate that this proposed methodology is comparable to traditional urban forest DBH measurements, with a root-mean-square error ranging from 0.7 to 2.4 cm. The proposed approach has been evaluated using real-world data, and it has been determined that the F-score assessment metric achieves a maximum of 0.91 in a university garden comprising 74 trees. The successful automated DBH measurements through SfM combined with fisheye lenses demonstrate the potential to improve urban tree inventories. Full article
(This article belongs to the Section Forest Inventory, Modeling and Remote Sensing)
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<p>Workflow of the proposed methodology.</p>
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<p>LG 360 camera R105 [<a href="#B41-forests-16-00439" class="html-bibr">41</a>].</p>
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<p>Visualization of camera calibration results: (<b>a</b>,<b>b</b>) depict vector field representations of distortion effects, illustrating the displacement of image points because of lens distortions. (<b>c</b>) Decentering distortion curve, showing pixel displacement as a function of the image radius. (<b>d</b>) Radial distortion curve, highlighting the increasing distortion effect toward the image periphery.</p>
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<p>Location of the study area.</p>
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<p>Field measurements: (<b>a</b>) tape measurement of the DBH, (<b>b</b>) image acquisition, and (<b>c</b>) RAD-coded targets with IDs.</p>
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<p>Histogram of residuals for check distances, showing the distribution of errors. The calculated mean residual (<math display="inline"><semantics> <mi>μ</mi> </semantics></math>) is −0.0005 m, and the standard deviation (<math display="inline"><semantics> <mi>σ</mi> </semantics></math>) is 0.001 m, indicating a high level of accuracy in the distance measurements.</p>
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<p>Axis transformation for georeferencing using reference and model point clouds. (<b>a</b>) The reference point cloud with designated control points (R1, R2) was used for alignment. (<b>b</b>) The model point cloud with corresponding points (A1, A2) was georeferenced relative to the reference cloud. The point-pair registration process refines the alignment accuracy, with the achievable RMS error indicating the precision of the transformation.</p>
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<p>Tree locations from a selected area of the dataset across four different datasets, showing field measurements and detected tree locations based on varying point cloud densities: (<b>a</b>) Manual Fit, (<b>b</b>) Medium-Density Model, (<b>c</b>) High-Density Model, and (<b>d</b>) Highest-Density Model.</p>
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<p>Overview of the generated point cloud: (<b>a</b>) top-down view showing camera locations (blue) and viewpoint direction (red triangle), and (<b>b</b>) perspective view of the dense point cloud representing tree trunks.</p>
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<p>The results of the SfM-based reconstruction of the “highest-density” point cloud (<b>a</b>) shows a perspective view of the point cloud; (<b>b</b>) highlights a section in blue, and other points are shown in red. (<b>c</b>) The results of the cylinder-fitted reference data with their corresponding measurements.</p>
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<p>Scatter plots comparing DBH estimates from different methods with field measurements. The red line represents the linear regression fit, and the <math display="inline"><semantics> <msup> <mi>R</mi> <mn>2</mn> </msup> </semantics></math> value indicates the strength of the relationship between the two variables in the estimated and field DBH correlation plots: (<b>a</b>) Manual Fit vs. Field Measurements, (<b>b</b>) Medium-Density Model vs. Field Measurements, (<b>c</b>) High-Density Model vs. Field Measurements, and (<b>d</b>) Highest-Density Model vs. Field Measurements.</p>
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22 pages, 4474 KiB  
Article
Advancing Stem Volume Estimation Using Multi-Platform LiDAR and Taper Model Integration for Precision Forestry
by Yongkyu Lee and Jungsoo Lee
Remote Sens. 2025, 17(5), 785; https://doi.org/10.3390/rs17050785 - 24 Feb 2025
Viewed by 190
Abstract
Stem volume is a critical factor in managing and evaluating forest resources. At present, stem volume is commonly estimated indirectly by constructing a taper model that utilizes sampling, diameter at breast height (DBH), and tree height. However, these estimates are constrained by errors [...] Read more.
Stem volume is a critical factor in managing and evaluating forest resources. At present, stem volume is commonly estimated indirectly by constructing a taper model that utilizes sampling, diameter at breast height (DBH), and tree height. However, these estimates are constrained by errors arising from spatial and stand environment variations as well as uncertainties in height measurements. To address these issues, this study aimed to accurately estimate stem volume using light detection and ranging (LiDAR) technology, a key tool in modern precision forestry. LiDAR data were used to build comprehensive three-dimensional models of forests with multi-platform LiDAR systems. This approach allowed for precise measurements of tree heights and stem diameters at various heights, effectively mitigating the limitations of earlier measurement methods. Based on these data, a Kozak taper curve was developed at the individual tree level using LiDAR-derived tree height and diameter estimates. Integrating this curve with LiDAR data enabled a hybrid approach to estimating stem volume, facilitating the calculation of diameters at points not directly identifiable from LiDAR data alone. The proposed method was implemented and evaluated for two economically significant tree species in Korea: Pinus koraiensis and Larix kaempferi. The RMSE comparison between the taper curve-based approach and the hybrid volume estimation method showed that, for Pinus koraiensis, the RMSE was 0.11 m3 using the taper curve-based approach and 0.07 m3 for the hybrid method, while for Larix kaempferi, the RMSE was 0.13 m3 and 0.05 m3, respectively. The estimation error of the hybrid method was approximately half that of the taper curve-based approach. Consequently, the hybrid volume estimation method, which integrates LiDAR and the taper model, overcomes the limitations of conventional taper curve-based approaches and contributes to improving the accuracy of forest resource monitoring. Full article
(This article belongs to the Special Issue Remote Sensing-Assisted Forest Inventory Planning)
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<p>Location of the study area: academic forest of Kangwon National University (Kangwon-do, Republic of Korea).</p>
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<p>Selection and spatial distribution of reference trees by species considering diameter at breast height and height.</p>
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<p>Flowchart for advancing stem volume estimation using multi-platform LiDAR and taper model integration [<a href="#B13-remotesensing-17-00785" class="html-bibr">13</a>].</p>
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<p>Comparison of TLS and ULS LiDAR data collection results for <span class="html-italic">P. koraiensis</span> and <span class="html-italic">L. kaempferi</span>.</p>
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<p>Reference tree data collection.</p>
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<p>Schematic of stem data construction and diameter estimation at various heights using LiDAR data.</p>
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<p>Comparison of accuracy between Vertex-measured height and LiDAR-based height relative to reference tree data.</p>
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<p>Distribution of diameter residuals by relative height and predicted diameter for each species: comparison between LiDAR-based taper model (LD) and standard volume table-based taper model (SD).</p>
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<p>Evaluation of stem volume estimation accuracy using standard and LiDAR methods.</p>
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12 pages, 3503 KiB  
Proceeding Paper
One-Node One-Edge Dimension-Balanced Hamiltonian Problem on Toroidal Mesh Graph
by Yancy Yu-Chen Chang and Justie Su-Tzu Juan
Eng. Proc. 2025, 89(1), 17; https://doi.org/10.3390/engproc2025089017 - 23 Feb 2025
Viewed by 51
Abstract
Given a graph G = (V, E), the edge set can be partitioned into k dimensions, for a positive integer k. The set of all i-dimensional edges of G is a subset of E(G) denoted [...] Read more.
Given a graph G = (V, E), the edge set can be partitioned into k dimensions, for a positive integer k. The set of all i-dimensional edges of G is a subset of E(G) denoted by Ei. A Hamiltonian cycle C on G contains all vertices on G. Let Ei(C) = E(C) ∩ Ei. For any 1 ≤ ik, C is called a dimension-balanced Hamiltonian cycle (DBH, for short) on G if ||Ei(C)| − |Ej(C)|| ≤ 1 for all 1 ≤ i < jk. The dimension-balanced cycle problem is generated with the 3-D scanning problem. Graph G is called p-node q-edge dimension-balanced Hamiltonian (p-node q-edge DBH) if it has a DBH after removing any p nodes and any q edges. G is called h-fault dimension-balanced Hamiltonian (h-fault DBH, for short) if it remains Hamiltonian after removing any h node and/or edges. The design for the network-on-chip (NoC) problem is important. One of the most famous NoC is the toroidal mesh graph Tm,n. The DBC problem on toroidal mesh graph Tm,n is appropriate for designing simple algorithms with low communication costs and avoiding congestion. Recently, the problem of a one-fault DBH on Tm,n has been studied. This paper solves the one-node one-edge DBH problem in the two-fault DBH problem on Tm,n. Full article
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<p><span class="html-italic">T</span><sub>4,3</sub>.</p>
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<p>(<b>a</b>,<b>b</b>) Two DBHs on <span class="html-italic">T</span><sub>3,3</sub> − <span class="html-italic">F</span>; (<b>c</b>,<b>d</b>) two DBHs on <span class="html-italic">T</span><sub>3,4</sub> − <span class="html-italic">F</span>.</p>
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<p>(<b>a</b>–<b>f</b>) Six different extended DBHs of <a href="#engproc-89-00017-f002" class="html-fig">Figure 2</a>c,d.</p>
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<p>If <span class="html-italic">F</span> = {(1, 0), (0, 0)(2, 0)}, the edges must be used.</p>
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<p>(<b>a</b>) A DBH of <span class="html-italic">T</span><sub>5,4</sub> − <span class="html-italic">F</span>; (<b>b</b>) another DBH of <span class="html-italic">T</span><sub>5,4</sub> − <span class="html-italic">F</span>.</p>
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<p>The constructed DBH <span class="html-italic">C</span><sub>1</sub> on <span class="html-italic">T</span><sub>7,4</sub> − <span class="html-italic">F</span>.</p>
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<p>Constructed DBH <span class="html-italic">C</span><sub>2</sub> on <span class="html-italic">T</span><sub>7,4</sub> − <span class="html-italic">F</span>.</p>
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<p>The constructed DBH <span class="html-italic">C</span><sub>1</sub> of <span class="html-italic">T</span><sub>9,4</sub> − <span class="html-italic">F</span>.</p>
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<p>The constructed DBH <span class="html-italic">C</span><sub>2</sub> of <span class="html-italic">T</span><sub>9,4</sub> − <span class="html-italic">F</span>.</p>
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<p>Examples for case 1.1 of Step 2 of Theorem 4. (<b>a</b>) <span class="html-italic">Z</span><sub>5</sub>, (<b>b</b>)<span class="html-italic">Z</span><sub>9</sub>, (<b>c</b>) a Hamiltonian cycle <span class="html-italic">C</span> on <span class="html-italic">T</span><sub>5,6</sub> − <span class="html-italic">F</span>, (<b>d</b>) a Hamiltonian cycle <span class="html-italic">C</span> on <span class="html-italic">T</span><sub>9,6</sub> − <span class="html-italic">F</span>, (<b>e</b>) the construct DBH of <span class="html-italic">T</span><sub>5,6</sub> − <span class="html-italic">F</span>, (<b>f</b>) the construct DBH of <span class="html-italic">T</span><sub>5,10</sub> − <span class="html-italic">F</span>.</p>
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<p>Examples for case 1.2 of Step 2 of Theorem 4. (<b>a</b>) <span class="html-italic">Z</span><sub>5</sub>, (<b>b</b>)<span class="html-italic">Z</span><sub>9</sub>, (<b>c</b>) a Hamiltonian cycle <span class="html-italic">C</span> on <span class="html-italic">T</span><sub>5,6</sub> − <span class="html-italic">F</span>, (<b>d</b>) a Hamiltonian cycle <span class="html-italic">C</span> on <span class="html-italic">T</span><sub>9,6</sub> − <span class="html-italic">F</span>, (<b>e</b>) the construct DBH of <span class="html-italic">T</span><sub>5,6</sub> − <span class="html-italic">F</span>, (<b>f</b>) the construct DBH of <span class="html-italic">T</span><sub>9,6</sub> − <span class="html-italic">F</span>, (<b>g</b>) the construct DBH of <span class="html-italic">T</span><sub>5,10</sub> − <span class="html-italic">F</span>, (<b>h</b>) the construct DBH of <span class="html-italic">T</span><sub>9,10</sub> − <span class="html-italic">F</span>.</p>
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<p>Examples for case 2.1 of Step 2 of Theorem 4. (<b>a</b>) <span class="html-italic">Z</span><sub>7</sub>, (<b>b</b>)<span class="html-italic">Z</span><sub>11</sub>, (<b>c</b>) a Hamiltonian cycle <span class="html-italic">C</span> on <span class="html-italic">T</span><sub>7,6</sub> − <span class="html-italic">F</span>, (<b>d</b>) a Hamiltonian cycle <span class="html-italic">C</span> on <span class="html-italic">T</span><sub>11,6</sub> − <span class="html-italic">F</span>, (<b>e</b>) the construct DBH of <span class="html-italic">T</span><sub>7,6</sub> − <span class="html-italic">F</span>, (<b>f</b>) the construct DBH of <span class="html-italic">T</span><sub>7,10</sub> − <span class="html-italic">F</span>.</p>
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<p>Examples for case 2.2 of Step 2 of Theorem 4. (<b>a</b>) <span class="html-italic">Z</span><sub>7</sub>, (<b>b</b>)<span class="html-italic">Z</span><sub>11</sub>, (<b>c</b>) a Hamiltonian cycle <span class="html-italic">C</span> on <span class="html-italic">T</span><sub>7,6</sub> − <span class="html-italic">F</span>, (<b>d</b>) a Hamiltonian cycle <span class="html-italic">C</span> on <span class="html-italic">T</span><sub>11,6</sub> − <span class="html-italic">F</span>, (<b>e</b>) the construct DBH of <span class="html-italic">T</span><sub>7,6</sub> − <span class="html-italic">F</span>, (<b>f</b>) the construct DBH of <span class="html-italic">T</span><sub>7,10</sub> − <span class="html-italic">F</span>.</p>
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27 pages, 11161 KiB  
Article
Quantifying Tree Structural Change in an African Savanna by Utilizing Multi-Temporal TLS Data
by Tasiyiwa Priscilla Muumbe, Jussi Baade, Pasi Raumonen, Corli Coetsee, Jenia Singh and Christiane Schmullius
Remote Sens. 2025, 17(5), 757; https://doi.org/10.3390/rs17050757 - 22 Feb 2025
Viewed by 252
Abstract
Structural changes in savanna trees vary spatially and temporally because of both biotic and abiotic drivers, as well as the complex interactions between them. Given this complexity, it is essential to monitor and quantify woody structural changes in savannas efficiently. We implemented a [...] Read more.
Structural changes in savanna trees vary spatially and temporally because of both biotic and abiotic drivers, as well as the complex interactions between them. Given this complexity, it is essential to monitor and quantify woody structural changes in savannas efficiently. We implemented a non-destructive approach based on Terrestrial Laser Scanning (TLS) and Quantitative Structure Models (QSMs) that offers the unique advantage of investigating changes in complex tree parameters, such as volume and branch length parameters that have not been previously reported for savanna trees. Leaf-off multi-scan TLS point clouds were acquired during the dry season, using a Riegl VZ1000 TLS, in September 2015 and October 2019 at the Skukuza flux tower in Kruger National Park, South Africa. These three-dimensional (3D) data covered an area of 15.2 ha with an average point density of 4270 points/m2 (0.015°) and 1600 points/m2 (0.025°) for the 2015 and 2019 clouds, respectively. Individual tree segmentation was applied on the two clouds using the comparative shortest-path algorithm in LiDAR 360(v5.4) software. We reconstructed optimized QSMs and assessed tree structural parameters such as Diameter at Breast Height (DBH), tree height, crown area, volume, and branch length at individual tree level. The DBH, tree height, crown area, and trunk volume showed significant positive correlations (R2 > 0.80) between scanning periods regardless of the difference in the number of points of the matched trees. The opposite was observed for total and branch volume, total number of branches, and 1st-order branch length. As the difference in the point densities increased, the difference in the computed parameters also increased (R2 < 0.63) for a high relative difference. A total of 45% of the trees present in 2015 were identified in 2019 as damaged/felled (75 trees), and the volume lost was estimated to be 83.4 m3. The results of our study showed that volume reconstruction algorithms such as TreeQSMs and high-resolution TLS datasets can be used successfully to quantify changes in the structure of savanna trees. The results of this study are key in understanding savanna ecology given its complex and dynamic nature and accurately quantifying the gains and losses that could arise from fire, drought, herbivory, and other abiotic and biotic disturbances. Full article
(This article belongs to the Special Issue Remote Sensing of Savannas and Woodlands II)
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<p>Study area location (<b>left</b>) showing the scanned area marked in black and the matched tree area marked in yellow and (<b>right</b>) the map of Kruger National Park showing the location of Skukuza Flux Tower where scanning was conducted (<b>a</b>) showing a cross-section displaying the change in vegetation over the 4-year period (<b>b</b>) 2015 and (<b>c</b>) 2019.</p>
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<p>Number of correctly segmented trees (<b>a</b>) 178 trees segmented in 2015 (<b>b</b>) 168 trees segmented in 2019, 93 standing and 75 felled or damaged (<b>c</b>) 75 damaged in 2019 matched to standing trees in 2015.</p>
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<p>The location of the 53 matched trees in the study area.</p>
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<p>Difference of points per tree between the matched trees. Very high (&gt;75%), high (50–75%), Medium (25–50%), and Low (≤25%) relative difference.</p>
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<p>Canopy Height Models Differences (<b>a</b>) 2019 CHM (<b>b</b>) 2015 CHM (<b>c</b>) Δ CHM = 2019 CHM-2015 CHM.</p>
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<p>Comparison between tree parameters between the two scanning periods (<b>a</b>) DBH (<b>b</b>) Tree Height (<b>c</b>) Crown Area (<b>d</b>) Trunk Volume (<b>e</b>) Total Volume (<b>f</b>) Branch Volume (<b>g</b>) Branch Length and (<b>h</b>) Branch length 1st-order branches. The dashed line is the 1:1 line, and the grey area represents the 95% confidence interval, while the black solid line represents the linear regression between the tree structural parameters in 2019 and 2015. <span class="html-italic">n</span> = 53. The error bars indicate the standard deviation of 10 reconstructions.</p>
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<p>Comparison between tree parameters between the two scanning periods (<b>a</b>) DBH (<b>b</b>) Tree Height (<b>c</b>) Crown Area (<b>d</b>) Trunk Volume (<b>e</b>) Total Volume (<b>f</b>) Branch Volume (<b>g</b>) Branch Length and (<b>h</b>) Branch length 1st-order branches. The dashed line is the 1:1 line, and the grey area represents the 95% confidence interval, while the black solid line represents the linear regression between the tree structural parameters in 2019 and 2015. <span class="html-italic">n</span> = 53. The error bars indicate the standard deviation of 10 reconstructions.</p>
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<p>Comparison between tree parameters between the two scanning periods (<b>a</b>) DBH (<b>b</b>) Tree Height (<b>c</b>) Crown Area (<b>d</b>) Trunk Volume (<b>e</b>) Total Volume (<b>f</b>) Branch Volume (<b>g</b>) Branch Length and (<b>h</b>) Branch length 1st-order branches. The dashed line is the 1:1 line, and the grey area represents the 95% confidence interval, while the black solid line represents the linear regression between the tree structural parameters in 2019 and 2015. <span class="html-italic">n</span> = 53. The error bars indicate the standard deviation of 10 reconstructions.</p>
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<p>Mean DBH in DBH Classes.</p>
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<p>Mean Tree Height in DBH Classes.</p>
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<p>Mean Crown Area in DBH Classes.</p>
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<p>Mean Trunk Volume in DBH Classes.</p>
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<p>Mean Total Volume in DBH Classes.</p>
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<p>Mean Branch Volume in DBH Classes.</p>
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<p>Mean Branch Length in DBH Classes.</p>
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<p>Mean 1st-order Branch Length in DBH Classes.</p>
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<p>Comparison between the TLS-derived and the field-measured DBH for 38 trees for both scanning periods. The dashed line is the 1:1 line, and the grey area represents the 95% confidence interval, while the blue and red solid lines represent the linear regression between the TLS-measured and field-measured DBH for 2015 and 2019, respectively.</p>
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<p>(<b>a</b>) An example of a tree that succumbed to the effects of elephant damage. The image shows the trees standing in 2015 (blue), and on the ground in 2019 (yellow) (<b>b</b>) An example of a tree that succumbed to the effects of drought—the image shows the tree with a crown in 2015 (blue) and having lost most of its crown in 2019 (yellow).</p>
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<p>Example of a matched tree that had a high relative difference (50–75%) in the number of points (<b>a</b>) The tree in 2015 and the resulting QSM model (51 200 points) (<b>b</b>) The tree in 2019 and the resulting QSM Model (115 700 points).</p>
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<p>Example of a matched tree that had a high relative difference (50–75%) in the number of points (<b>a</b>) The tree in 2015 and the resulting QSM model (51 200 points) (<b>b</b>) The tree in 2019 and the resulting QSM Model (115 700 points).</p>
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18 pages, 2133 KiB  
Article
Impact of Reflective Ground Film on Fruit Quality, Condition, and Post-Harvest of Sweet Cherry (Prunus avium L.) cv. Regina Cultivated Under Plastic Cover in Southern Chile
by Ariel Muñoz-Alarcón, Cristóbal Palacios-Peralta, Jorge González-Villagra, Nicolás Carrasco-Catricura, Pamela Osorio and Alejandra Ribera-Fonseca
Agronomy 2025, 15(3), 520; https://doi.org/10.3390/agronomy15030520 - 21 Feb 2025
Viewed by 178
Abstract
Plastic covers protect fruits from cracking caused by pre-harvest rains in sweet cherry orchards; however, they can decrease the quality parameters of cherries, such as firmness, titratable acidity, color, and sugar content. This study evaluated the impact of a reflective ground film used [...] Read more.
Plastic covers protect fruits from cracking caused by pre-harvest rains in sweet cherry orchards; however, they can decrease the quality parameters of cherries, such as firmness, titratable acidity, color, and sugar content. This study evaluated the impact of a reflective ground film used for 21 or 34 DBH (days before harvest) in a commercial sweet cherry orchard (cv. Regina) grown under plastic cover in southern Chile. Our study showed that the exposition of cherry trees to the reflective film increased firmness and total soluble solid (TSS) content in fruits at harvest, homogenizing the concentration of sugars in fruits along the tree canopy. Additionally, using reflective film for 21 DBH increased the proportion of fruits greater than 32 mm in the upper canopy and the quantity of mahogany-colored cherries in the lower canopy, compared to trees un-exposed to the reflective film. Concerning fruit condition defects, the results reveal that using the reflective film increased the incidence of cracking in fruits in both the upper and lower zones of the canopy. Furthermore, we found that the incidence of orange skin and pitting in fruits decreased at post-harvest in trees exposed to the reflective film, but depending on the canopy zones. Moreover, fruits of trees exposed to the film for 34 DBH exhibited a higher incidence of browning pedicel post-harvest. Finally, according to our results, the antioxidant activity increased in fruits exposed to the reflective film for 21 DBH. Therefore, we can conclude that using reflective films on sweet cherry orchards can improve and homogenize the maturity parameters and the antioxidant activity of fruits; however, this practice can negatively impact the condition of fruits post-harvest. Full article
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<p>(<b>A</b>) Reflective film installed in the sweet cherry orchard and (<b>B</b>) diagram of the experimental design used for the study.</p>
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<p>Fruit size (mm) distribution at harvest in lower (<b>A</b>), upper (<b>B</b>), and total canopy zones (<b>C</b>) of sweet cherries (cv. Regina). In white, the control (without reflective film); in gray, the reflective film treatment at 21 DBH; and in black, the reflective film treatment at 34 DBH. Different letters per category indicate a statistical difference between the treatments (<span class="html-italic">p</span> &lt; 0.05). n.d.: not detected. DBH: days before harvest.</p>
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<p>Fruit color distribution at harvest in lower (<b>A</b>), upper (<b>B</b>), and total canopy zone (<b>C</b>) of sweet cherries (cv. Regina). In white, the control (without the reflective film); in gray, the reflective film treatment at 21 DBH; and in black, the reflective film treatment at 34 DBH. Different letters per category indicate statistical differences between the treatments (<span class="html-italic">p</span> &lt; 0.05). DBH: days before harvest.</p>
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<p>Fruit color distribution at harvest in lower (<b>A</b>), upper (<b>B</b>), and total canopy zone (<b>C</b>) of sweet cherries (cv. Regina). In white, the control (without the reflective film); in gray, the reflective film treatment at 21 DBH; and in black, the reflective film treatment at 34 DBH. Different letters per category indicate statistical differences between the treatments (<span class="html-italic">p</span> &lt; 0.05). DBH: days before harvest.</p>
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<p>Cracking incidence (%) at harvest in lower (<b>A</b>), upper (<b>B</b>), and total canopy zones (<b>C</b>) of sweet cherries (cv. Regina). In white, the control (without reflective film); in gray, the reflective film treatment at 21 DBH; and in black, the reflective film treatment at 34 DBH. Differences between canopy zones for each treatment are represented by vertical lowercase letters based on Fisher’s LSD multiple range test (<span class="html-italic">p</span> ≤ 0.05). n.d.: not detected.</p>
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<p>Correlation of antioxidant activity (DPPH method) and total phenols concentration in the skin (<b>A</b>) and pulp (<b>B</b>) of sweet cherries at harvest (cv. Regina).</p>
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<p>Orange peel (<b>A</b>), pitting (<b>B</b>), and browning (<b>C</b>) incidences at post-harvest of sweet cherries (cv. Regina) subjected to one control (without reflective film), one treatment with reflective film placed at 21 DBH, and a second treatment with reflective film placed at 34 DBH. Statistically significant differences between treatments for each treatment are represented by different lowercase letters based on Fisher’s LSD multiple range test (<span class="html-italic">p</span> ≤ 0.05).</p>
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<p>Fruit condition at post-harvest of sweet cherries (cv. Regina) subjected to one control (without reflective film) (<b>A</b>), one treatment with reflective film placed at 21 DBH (<b>B</b>), and a second treatment with reflective film placed at 34 DBH (<b>C</b>).</p>
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26 pages, 14273 KiB  
Article
Improving National Forest Mapping in Romania Using Machine Learning and Sentinel-2 Multispectral Imagery
by Mohamed Islam Keskes, Aya Hamed Mohamed, Stelian Alexandru Borz and Mihai Daniel Niţă
Remote Sens. 2025, 17(4), 715; https://doi.org/10.3390/rs17040715 - 19 Feb 2025
Viewed by 237
Abstract
Forest attributes, such as standing stock, diameter at breast height (DBH), tree height, and basal area, are critical for effective forest management; yet, traditional estimation methods remain labor-intensive and often lack the spatial detail required for contemporary decision-making. This study addresses these challenges [...] Read more.
Forest attributes, such as standing stock, diameter at breast height (DBH), tree height, and basal area, are critical for effective forest management; yet, traditional estimation methods remain labor-intensive and often lack the spatial detail required for contemporary decision-making. This study addresses these challenges by integrating machine learning algorithms with high-resolution remotely sensed data and rigorously collected ground truth measurements to produce accurate, national-scale maps of forest attributes in Romania. To ensure the reliability of the model predictions, extensive field campaigns were conducted across representative Romanian forests. During these campaigns, detailed measurements were recorded for every tree within selected plots. For each tree, DBH was measured directly, and tree heights were obtained either by direct measurement—using hypsometers or clinometers—or, when direct measurements were not feasible, by applying well-established DBH—height allometric relationships that have been calibrated for the local forest types. This comprehensive approach to ground data collection, supplemented by an independent dataset from Brasov County collected using the same protocols, allowed for robust training and validation of the machine learning models. This study evaluates the performance of three machine learning algorithms—Random Forest (RF), Classification and Regression Trees (CART), and the Gradient Boosting Tree Algorithm (GBTA)—in predicting the forest attributes from Sentinel-2 satellite imagery. While Random Forest consistently delivered high R2 values and low root mean square errors (RMSE) across all attributes, GBTA showed particular strength in predicting standing stock, and CART excelled in basal area estimation but was less reliable for other attributes. A sensitivity analysis across multiple spatial resolutions revealed that the performance of all algorithms varied significantly with changes in resolution, emphasizing the importance of selecting an appropriate scale for accurate forest mapping. By focusing on both the methodological advancements in machine learning applications and the rigorous, detailed empirical forest data collection, this study provides a clear solution to the problem of obtaining reliable, spatially detailed forest attribute maps. Full article
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<p>Study area’s geographic location: (<b>A</b>) Romanian forests at continental scale; (<b>B</b>) Plots locations as red dots used at national scale; (<b>C</b>) Individual tree measurements used for independent validation in Lempes test area.</p>
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<p>Workflow diagram for data used in research.</p>
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<p>Forest characteristics modeling results at national level.</p>
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<p>Scatterplots for the GBTA regression model of forest stand attributes: (<b>a</b>) BA; (<b>b</b>) Vol; (<b>c</b>) DBH; (<b>d</b>) H.</p>
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<p>Scatterplots for the RF regression model of forest stand attributes: (<b>a</b>) BA; (<b>b</b>) Vol; (<b>c</b>) DBH; (<b>d</b>) H.</p>
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<p>Scatterplots for the CART regression model of forest attributes: (<b>a</b>) BA; (<b>b</b>) Vol; (<b>c</b>) DBH; (<b>d</b>) H.</p>
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<p>Forest Characteristic Mapping for the BA using (<b>a</b>) RF, (<b>b</b>) GBTA, (<b>c</b>) CART, (<b>d</b>) Field measurements.</p>
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<p>Forest Characteristic Mapping for the Vol using (<b>a</b>) RF, (<b>b</b>) GBTA, (<b>c</b>) CART, (<b>d</b>) Field measurements.</p>
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<p>Forest Characteristic Mapping for the H using (<b>a</b>) RF, (<b>b</b>) GBTA, (<b>c</b>) CART, (<b>d</b>) Field measurements.</p>
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<p>Forest Characteristic Mapping for the DBH using (<b>a</b>) RF, (<b>b</b>) GBTA, (<b>c</b>) CART, (<b>d</b>) Field measurements.</p>
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<p>Scatterplots for the model’s performance with the test area for BA prediction under different resolutions.</p>
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<p>Scatterplots for the model’s performance with the test area for H prediction under different resolutions.</p>
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<p>Scatterplots for the model’s performance with the test area for DBH prediction under different resolutions.</p>
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<p>Scatterplots for the model’s performance with the test area for Vol prediction under different resolutions.</p>
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<p>Comparison between NFI volume measurements at national and regional levels against predicted models, using (<b>a</b>) RF, (<b>b</b>) GBTA, (<b>c</b>) CART.</p>
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<p>Distribution of plot clusters containing the number of plots used for training.</p>
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22 pages, 4836 KiB  
Article
Riparian Forest Health Assessment in the Valley Area of the Irtysh River Basin
by Ye Yuan, Hongbin Li, Hanyue Wang, Tong Liu, Zhifang Xue, Jihu Song and Ling Xu
Forests 2025, 16(2), 373; https://doi.org/10.3390/f16020373 - 19 Feb 2025
Viewed by 237
Abstract
Riparian forests in the valley area of the Irtysh River Basin are capable of providing a variety of ecosystem services such as water conservation and biodiversity maintenance. Their health condition is an important reflection of their ability to maintain the stability of ecosystem [...] Read more.
Riparian forests in the valley area of the Irtysh River Basin are capable of providing a variety of ecosystem services such as water conservation and biodiversity maintenance. Their health condition is an important reflection of their ability to maintain the stability of ecosystem structure and perform ecosystem functions. In this study, a comprehensive survey was conducted to observe the typical distribution areas of riparian forests in the valley of six tributaries and one main stream of the Irtysh River Basin. Twelve indicators were chosen from the three categories of vigor (i.e., productivity), organization (i.e., species diversity and structure complexity), and resistance (i.e., harmful factors and disturbances) to form an evaluation system. Expert-based and statistical weighting were applied to calculate the health scores of riparian forests in the valley and prioritized the health grades of seven rivers. Several criteria were used to further classify the unhealthy level of each river individually. The results of this study can be used as a foundation for future conservation and orderly development of riparian forests in the valley area. The results show that (1) the Kuyertes River was classified as healthy, while the Haba and Berezek Rivers were classified as unhealthy. (2) Among the three evaluation categories, the organization consistently achieved higher scores compared to vigor and resistance. (3) Unhealthy conditions were consistently observed in the midstream sections of each river. (4) Forest types such as Salix alba L. forests, Populus euphratica Oliv. forests, and Betula pendula Roth forests were particularly prone to poor health outcomes. The health of the riparian forests was relatively unsatisfactory due to the conflicting water resource allocation. The protection and restoration of riparian forests in the valley area of the Haba and Berezek Rivers should be prioritized in the future, as well as the middle reaches of each tributary. Additionally, it is necessary to pay attention to three key indicators: stand volume per unit area, stand density, and diameter at breast high (dbh) class structure to improve the health condition of riparian forests. Full article
(This article belongs to the Section Forest Health)
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<p>Locations and vegetation types of sampled plots in the riparian forests of the Irtysh River Basin.</p>
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<p>Investigation of the unhealthy characteristics of the riparian forests community. Photographs from left to right showed three states of unhealthy trees: (1) trees with lateral branches beginning to dry up, (2) completely standing dead trees and (3) fallen wood.</p>
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<p>VOR conceptual model in this study.</p>
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<p>Health scores of riparian forests for the overall rivers in the Irtysh River Basin.</p>
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<p>Health grades of riparian forests in the valley area of the main stream and six tributaries. The six tributaries are the Berezek River (<b>a</b>), Haba River (<b>b</b>), Kuyertes River (left) and Kayertes Rivers (right) (<b>c</b>), Crane River (<b>d</b>), and Burqin River (<b>e</b>). The main stream is the Irtysh River (<b>f</b>). The numbers labeled in the figure were the highest and lowest riparian forest health scores for each river. The pink-shaded areas showed the distribution of broadleaf forests [<a href="#B46-forests-16-00373" class="html-bibr">46</a>].</p>
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<p>Health grades of riparian forests in different forest types across the overall rivers and individual rivers.</p>
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9 pages, 832 KiB  
Brief Report
Effect of Fertilization on the Performance of Adult Pinus pinea Trees
by Verónica Loewe-Muñoz, Claudia Bonomelli, Claudia Delard, Rodrigo Del Río and Monica Balzarini
Biology 2025, 14(2), 216; https://doi.org/10.3390/biology14020216 - 19 Feb 2025
Viewed by 243
Abstract
Background: Pinus pinea L. (stone pine) produces pine nuts of high value. Its cultivation is carried out in forests and plantations, with intensive management techniques being studied to stimulate diameter growth, which is positively related to cone production. Aims: To evaluate the effect [...] Read more.
Background: Pinus pinea L. (stone pine) produces pine nuts of high value. Its cultivation is carried out in forests and plantations, with intensive management techniques being studied to stimulate diameter growth, which is positively related to cone production. Aims: To evaluate the effect of fertilization in a 30-year-old plantation and to understand if adult trees respond to nutritional management. Methods: A trial with completely randomized block design was established with two treatments (fertilization/control) and three repetitions. The plantation, with a density of 204 trees/ha, is located in central Chile, on a sandy-loam soil with neutral pH, medium organic matter content, and a fertility condition that limits tree development. Fertilization considered the repeated application of macro (N, P, K, S, Mg) and micronutrients (B, Fe, and Zn). Periodic measurements of height, stem and crown diameter, and cone production were made up to age 36. Cone production was evaluated using mixed generalized linear models and growth variables using ANOVA (analysis of variance). Results: Significant effects of fertilization on DBH annual growth (35% higher than the control, p < 0.001) and in cone production (3 times higher, p < 0.0001) were found. Conclusions: Fertilization is a useful practice to improve the growth and cone productivity of the species. Full article
(This article belongs to the Special Issue Dendrochronology in Arid and Semiarid Regions)
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<p>Stone pine tree size evolution from the experiment establishment in a plantation located in central Chile (blue: fertilization, red: control). Bars show standard errors.</p>
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<p>Stone pine tree size evolution from the experiment establishment in a plantation located in central Chile (blue: fertilization, red: control). Bars show standard errors.</p>
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<p>Cone production in 36-year-old stone pine trees in 2019, six years after the fertilization experiment started, in a xeric environment under drought. Bars show standard errors. Different letters indicate statistical differences among treatments at α = 0.05.</p>
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23 pages, 6070 KiB  
Article
Harnessing Backpack Lidar Technology: A Novel Approach to Monitoring Moso Bamboo Shoot Growth
by Chen Li, Chong Li, Chunyu Pan, Yancun Yan, Yufeng Zhou, Jingyi Sun and Guomo Zhou
Forests 2025, 16(2), 371; https://doi.org/10.3390/f16020371 - 19 Feb 2025
Viewed by 421
Abstract
Bamboo, characterized by its high growth speed and short maturation period, occupies 0.875% of the global forest area and significantly contributes to terrestrial carbon cycling. The state of shoot growth can essentially indicate a bamboo forests’ health and productivity. This study explored the [...] Read more.
Bamboo, characterized by its high growth speed and short maturation period, occupies 0.875% of the global forest area and significantly contributes to terrestrial carbon cycling. The state of shoot growth can essentially indicate a bamboo forests’ health and productivity. This study explored the potential of backpack laser scanning (BLS) for monitoring the growth of Moso bamboo shoots (Phyllostachys edulis), a key economic species in subtropical China. Initially, the accuracy of BLS in extracting attributes of bamboo and shoots (including diameter at breast height (DBH), height, and real-world coordinates) was validated. An optimized method was developed to address the lower precision of BLS in extracting the DBH for thinner species. Subsequently, this research analyzed the impact of spatial structure and other indicators on shoot emergence stage and growth rate using a random forest model. The results indicate that BLS can accurately extract Moso bamboo and shoot height (RMSE = 0.748 m) even in dense bamboo forests. After optimization, the error in DBH extraction significantly decreased (RMSE = 0.835 cm), with the average planar and elevation errors for Moso bamboo being 0.227 m and 0.132 m, respectively. The main indicators affecting the coordinate error of Moso bamboo were the distance to the start (DS) and the distance to the trajectory (DT). The emergence time of shoots was mainly influenced by the surrounding Moso bamboo quantity, with the leaf area index (LAI) and competition index (CI) positively related to the growth rate of shoots. The importance ranking of spatial structure for the carbon storage of shoots was similar to that of the growth rate of shoots, with both identifying LAI as the most significant indicator. This study has validated the value of BLS in monitoring the growth of shoots, providing a theoretical support for the sustainable management and conservation of bamboo forests. Full article
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<p>Overview of the study area: (<b>a</b>) Zhejiang Province; (<b>b</b>) Lin’an District; (<b>c</b>) location of eight sample plots (the black line indicates the plots range.); (<b>d</b>) sample plots’ situation; and (<b>e</b>) sample plot point cloud data (displayed by height).</p>
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<p>Trajectory of collecting BLS data and schematic diagram of Moso bamboo location. (<b>a</b>) The color gradient line indicates the collecting trajectory. (<b>b</b>) Demonstrating the results of Moso bamboo location and measuring coordinates from the uphill position (the red point cloud indicated by the arrow represents the localization point).</p>
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<p>Correlation between the height of bamboo and shoots extracted from BLS and the actual height (the orange line is the regression line fitted to the scatter data).</p>
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<p>The error between DBH extracted by BLS and measured DBH. (<b>a</b>) The difference between the extracted DBH and the measured DBH before optimization. (<b>b</b>) The difference between the extracted DBH and that after optimization.</p>
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<p>Correlation between extracted DBH and measured DBH during the growth process of shoots (the orange line represents the linear regression fit to the scatter data; the dashed line represents the 1:1 goodness-fit-line).</p>
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<p>Histogram of growth rates simulated using the Monte Carlo method.</p>
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<p>Distribution map of coordinates of Moso bamboo extracted by BLS and measured coordinates.</p>
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<p>Fitting curve of shoot growth: (<b>a</b>) the growth curves of shoots with different diameter class; (<b>b</b>) the growth curve of shoots at different emergence stages.</p>
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<p>The result of the ranking of the importance of six spatial structures on the carbon storage of shoots where the error bars represent the standard deviation of the output from the random forest model.</p>
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<p>The distribution of <span class="html-italic">RMSE</span> for the DBH during the growth period of shoots in sample plots (the circles in the figure represent outliers in the data).</p>
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<p>Results of the random forest model for Δ<span class="html-italic">E</span>, Δ<span class="html-italic">N</span>, Δ<span class="html-italic">H</span>, and Δ<span class="html-italic">P</span>. (<b>a</b>) Relative importance of variables, where the error bars represent the standard deviation of the output from the random forest model constructed by randomly drawing 80% of the samples 100 times. (<b>b</b>) The relationship between DS and Δ<span class="html-italic">P</span>, with the red line representing the fitted curve and the box plot indicating the distribution of Δ<span class="html-italic">P</span>. (<b>c</b>) The relationship between DT and Δ<span class="html-italic">P</span>, with the red line representing the fitted curve and the box plot indicating the distribution of Δ<span class="html-italic">P</span>.</p>
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<p>Results of the random forest model for the emergence stage and average growth rate of shoots. (<b>a</b>) The relative importance of variables for the emergence stage of shoots, where the error bars represent the standard deviation of the output from the random forest model constructed by randomly drawing 80% of the samples 100 times. (<b>b</b>) The relative importance of variables for the average growth rate of shoots, where the error bars represent the standard deviation of the output from the random forest model constructed by randomly drawing 80% of the samples 100 times.</p>
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29 pages, 12160 KiB  
Article
Integration of UAS and Backpack-LiDAR to Estimate Aboveground Biomass of Picea crassifolia Forest in Eastern Qinghai, China
by Junejo Sikandar Ali, Long Chen, Bingzhi Liao, Chongshan Wang, Fen Zhang, Yasir Ali Bhutto, Shafique A. Junejo and Yanyun Nian
Remote Sens. 2025, 17(4), 681; https://doi.org/10.3390/rs17040681 - 17 Feb 2025
Viewed by 340
Abstract
Precise aboveground biomass (AGB) estimation of forests is crucial for sustainable carbon management and ecological monitoring. Traditional methods, such as destructive sampling, field measurements of Diameter at Breast Height with height (DBH and H), and optical remote sensing imagery, often fall short in [...] Read more.
Precise aboveground biomass (AGB) estimation of forests is crucial for sustainable carbon management and ecological monitoring. Traditional methods, such as destructive sampling, field measurements of Diameter at Breast Height with height (DBH and H), and optical remote sensing imagery, often fall short in capturing detailed spatial heterogeneity in AGB estimation and are labor-intensive. Recent advancements in remote sensing technologies, predominantly Light Detection and Ranging (LiDAR), offer potential improvements in accurate AGB estimation and ecological monitoring. Nonetheless, there is limited research on the combined use of UAS (Uncrewed Aerial System) and Backpack-LiDAR technologies for detailed forest biomass. Thus, our study aimed to estimate AGB at the plot level for Picea crassifolia forests in eastern Qinghai, China, by integrating UAS-LiDAR and Backpack-LiDAR data. The Comparative Shortest Path (CSP) algorithm was employed to segment the point clouds from the Backpack-LiDAR, detect seed points and calculate the DBH of individual trees. After that, using these initial seed point files, we segmented the individual trees from the UAS-LiDAR data by employing the Point Cloud Segmentation (PCS) method and measured individual tree heights, which enabled the calculation of the observed/measured AGB across three specific areas. Furthermore, advanced regression models, such as Random Forest (RF), Multiple Linear Regression (MLR), and Support Vector Regression (SVR), are used to estimate AGB using integrated data from both sources (UAS and Backpack-LiDAR). Our results show that: (1) Backpack-LiDAR extracted DBH compared to field extracted DBH shows about (R2 = 0.88, RMSE = 0.04 m) whereas UAS-LiDAR extracted height achieved the accuracy (R2 = 0.91, RMSE = 1.68 m), which verifies the reliability of the abstracted DBH and height obtained from the LiDAR data. (2) Individual Tree Segmentation (ITS) using a seed file of X and Y coordinates from Backpack to UAS-LiDAR, attaining a total accuracy F-score of 0.96. (3) Using the allometric equation, we obtained AGB ranges from 9.95–409 (Mg/ha). (4) The RF model demonstrated superior accuracy with a coefficient of determination (R2) of 89%, a relative Root Mean Square Error (rRMSE) of 29.34%, and a Root Mean Square Error (RMSE) of 33.92 Mg/ha compared to the MLR and SVR models in AGB prediction. (5) The combination of Backpack-LiDAR and UAS-LiDAR enhanced the ITS accuracy for the AGB estimation of forests. This work highlights the potential of integrating LiDAR technologies to advance ecological monitoring, which can be very important for climate change mitigation and sustainable environmental management in forest monitoring practices. Full article
(This article belongs to the Special Issue Remote Sensing and Lidar Data for Forest Monitoring)
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<p>Maps of the selected <span class="html-italic">Picea crassifolia</span> forest study area and distribution of sample plots. (<b>a</b>) Location of the Qinghai−Tibetan Plateau in China. (<b>b</b>) The Digital Elevation Model (DEM) stands for the height from sea level (<b>c</b>) Normalized Difference Vegetation Index (NDVI) shows the vegetation distribution ratio map of three <span class="html-italic">Picea crassifolia</span> forest sites using Google Earth Engine (GEE) sentinel satellite images with Google Earth images showing the study area (<b>d</b>) LiDAR point cloud cross-sectional view of alignment; the black dots represent Backpack-LiDAR data, while chromatic dots show UAS-LiDAR data alignment of both platforms over each other.</p>
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<p>One plot of 30 × 30 m for the Backpack-LiDAR data acquisition method; the yellow lines are the distance from one end to the other end of the plot, and the red lines represent the data acquisition track.</p>
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<p>The schematic diagram for one plot data segmentation subdivided each 30 × 30 m plot into 10 × 10 m.</p>
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<p>The workflow overview for estimating forest aboveground biomass using LiDAR data.</p>
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<p>Data alignment of both platforms (Backpack and UAS-LiDAR): (<b>a</b>) Marking numbers for the same tree point cloud in the UAS and Backpack-LiDAR point clouds, (<b>b</b>) Normalizing 30 × 30 m LiDAR point clouds, (<b>c</b>) Cross section line on point clouds chromatic color represents Backpack while RGB is UAS point clouds, (<b>d</b>) Overlay maps of original Backpack-LiDAR (black) and UAS-LiDAR (chromatic) point, (<b>e</b>) Overlay maps of normalized Backpack-LiDAR and UAS-LiDAR point clouds. A yellow line in figure (<b>c</b>,<b>d</b>) represents cross-sectional region on point clouds from “A towards ”B at both ends.</p>
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<p>LiDAR 360 software interface for Backpack-LiDAR data segmentation; the result shows a trunk slice at 1.3 m, (<b>a</b>) Backpack-LiDAR point cloud data for a single tree. (<b>b</b>) Point cloud data fitted to DBH at 1.3 m. (<b>c</b>) Incorrectly classified trees manually corrected. (<b>d</b>) Segmentation results of Backpack-LiDAR point clouds.</p>
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<p>Comparisons between field-measured DBH, H, and extracted DBH from Backpack-LiDAR and H from UAS-LiDAR point cloud data. (<b>a</b>) DBH comparison, and (<b>b</b>) Height comparison.</p>
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<p>LiDAR variables essential for AGB prediction (<b>a</b>) The importance rank of the variable based on random forest. (<b>b</b>) Pearson correlation between LiDAR variables.</p>
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<p>Field-estimated Forest AGB (Mg/ha) versus predicted forest AGB (Mg/ha). (<b>a</b>) MLR model. (<b>b</b>) RF model (<b>c</b>) SVR model. The solid line represents the fitting model and the gray areas show the 95% confidence intervals of the fitting models.</p>
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<p>The performance comparison of MLR, RF, and SVR, using R<sup>2</sup>, RMSE, and rRMSE, where R<sup>2</sup> shows a random forest with the highest score of 89%.</p>
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<p>Graphical representation of the results for tree segmentation. Where (<b>a</b>) illustrates graphical results of Backpack-LiDAR point cloud data segmentation, (<b>b</b>) shows the results of the UAS-LiDAR data based on the segmentation results using seed points, and (<b>c</b>) demonstrates the segmentation results of the UAS-LiDAR data without seed points. Note that each color in the above figure characterizes a different tree species, whereas the polygon areas overlapped on (<b>b</b>,<b>c</b>) refer to the distinct trees and/or tree crowns resulting from the graphical illustration.</p>
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19 pages, 12245 KiB  
Article
Development of Full Growth Cycle Crown Width Models for Chinese Fir (Cunninghamia lanceolata) in Southern China
by Zheyuan Wu, Dongbo Xie, Ziyang Liu, Linyan Feng, Qiaolin Ye, Jinsheng Ye, Qiulai Wang, Xingyong Liao, Yongjun Wang, Ram P. Sharma and Liyong Fu
Forests 2025, 16(2), 353; https://doi.org/10.3390/f16020353 - 16 Feb 2025
Viewed by 336
Abstract
This study focused on 16,101 Cunninghamia lanceolata trees across 133 plots in seven cities of Guangdong Province, China, to develop a comprehensive full growth cycle crown width (CW) model. We systematically analyzed the dynamic characteristics of CW and its multi-scale influencing mechanisms. A [...] Read more.
This study focused on 16,101 Cunninghamia lanceolata trees across 133 plots in seven cities of Guangdong Province, China, to develop a comprehensive full growth cycle crown width (CW) model. We systematically analyzed the dynamic characteristics of CW and its multi-scale influencing mechanisms. A binary basic model, with the diameter at breast height (DBH) and height (H) as core predictor variables, effectively reflected tree growth patterns. The inclusion of age groups as dummy variables allowed the model to capture the dynamic changes in CW across different growth stages. Furthermore, the incorporation of a nested two-level nonlinear mixed-effects (NLME) model, accounting for random effects from the forest block- and sample plot-level effects, significantly improved the precision and applicability of the final model (R2 = 0.731, RMSE = 0.491). This model quantified both macro- and micro-level effects of region and plot on CW. Our findings showed that the two-level NLME model, incorporating tree age groups, optimally accounted for environmental heterogeneity and tree growth cycles, resulting in the best-fitting statistics. The proposed full growth cycle CW model effectively enhanced the model’s efficiency and predictive accuracy for Cunninghamia lanceolata, providing scientific support for the sustainable management and dynamic monitoring of plantation forests. Full article
(This article belongs to the Special Issue Forest Biometrics, Inventory, and Modelling of Growth and Yield)
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<p>Sample point distribution map.</p>
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<p>Methodology framework.</p>
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<p>Pearson’s correlation coefficients and collinearity tests among multiple variables and crown width: (<b>a</b>) correlation coefficients of CW with other variables; (<b>b</b>) collinearity of CW with each variable, where the y−axis shows the VIF values. CW, crown width; A, stand age (year); SD, stand density; CD, canopy closure; DBH, diameter at breast height; H, tree height; HCB, height to crown base; DH, dominant tree height.</p>
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<p>Comparison of model performance for predicting Chinese fir crown width (<span class="html-italic">RMSE</span>, Root Mean Square Error; <span class="html-italic">TRE</span>, Total Relative Error; blue points, training dataset; orange points, validation dataset).</p>
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<p>Distribution of residuals for three models predicting the CW of Chinese fir trees.</p>
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14 pages, 1142 KiB  
Article
Motor and Non-Motor Effects of Acute MPTP in Adult Zebrafish: Insights into Parkinson’s Disease
by Niki Tagkalidou, Marija Stevanović, Irene Romero-Alfano, Gustavo Axel Elizalde-Velázquez, Selene Elizabeth Herrera-Vázquez, Eva Prats, Cristian Gómez-Canela, Leobardo Manuel Gómez-Oliván and Demetrio Raldúa
Int. J. Mol. Sci. 2025, 26(4), 1674; https://doi.org/10.3390/ijms26041674 - 16 Feb 2025
Viewed by 271
Abstract
Parkinson’s disease (PD), the second most common neurodegenerative disorder, is characterized by the progressive loss of dopaminergic neurons in the substantia nigra pars compacta, leading to motor and non-motor symptoms. The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been extensively used in different animal species to [...] Read more.
Parkinson’s disease (PD), the second most common neurodegenerative disorder, is characterized by the progressive loss of dopaminergic neurons in the substantia nigra pars compacta, leading to motor and non-motor symptoms. The neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) has been extensively used in different animal species to develop chemical models of PD. This study aimed to evaluate the effects of acute exposure to MPTP (3 × 150 mg/kg, intraperitoneally) on adult zebrafish by assessing the neurochemical, transcriptional, and motor changes associated with PD pathogenesis. MPTP treatment resulted in a significant decrease in brain catecholamines, including dopamine, norepinephrine, and normetanephrine. Additionally, a trend towards decreased levels of dopamine precursors (tyrosine and L-DOPA) and degradation products (3-MT and DOPAC) was also observed, although these changes were not statistically significant. Gene expression analysis showed the downregulation of dbh, while the expression of other genes involved in catecholamine metabolism (th1, th2, mao, comtb) and transport (slc6a3 and slc18a2) remained unaltered, suggesting a lack of dopaminergic neuron degeneration. Behavioral assessments revealed that MPTP-exposed zebrafish exhibited reduced motor activity, consistent with the observed decrease in dopamine levels. In contrast, the kinematic parameters of sharp turning were unaffected. A significant impairment in the sensorimotor gating of the ASR was detected in the MPTP-treated fish, consistent with psychosis. Despite dopamine depletion and behavioral impairments, the absence of neurodegeneration and some hallmark PD motor symptoms suggests limitations in the validity of this model for fully recapitulating PD pathology. Further studies are needed to refine the use of MPTP in zebrafish PD models. Full article
(This article belongs to the Special Issue Zebrafish as a Model for Biomedical Studies—2nd Edition)
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<p>Profile of catecholaminergic neurotransmitters, precursors, and degradation products in the brains of control and MPTP-treated adult zebrafish. Results are presented as percentage of the control values. Boxplot representation of the content of each neurochemical expressed as percentage of the control, with the boxes indicating the 25th and 75th percentiles and the whiskers the maximum and minimum values, showing all data. *** <span class="html-italic">p</span> &lt; 0.001. Tyr: tyrosine; L-DOPA: levodopa; DA: dopamine; 3-MT: 3-Methoxytyramine; DOPAC: 3,4-Dihydroxyphenylacetic acid; NE: norepinephrine; NMN: normetanephrine.</p>
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<p>Acute exposure to MPTP leads to hypokinesia in adult zebrafish. Fish were videorecorded at different times for 10 min in the open field test paradigm and the total distance travelled was calculated. Data are reported as mean ± SEM (<span class="html-italic">n</span> = 23–24 for 24 h after the second and the third injections, <span class="html-italic">n</span> = 12 for 48 h after the third injection, and <span class="html-italic">n</span> = 8–12 for 72 h after the third injection). * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001; Student’s <span class="html-italic">t</span> test.</p>
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<p>Acute exposure to MPTP leads to a strong decrease in prepulse inhibition (PPI) percentage. The assay was performed using the platform Zebra_K, with 2 different interstimulus intervals between the prepulse and the pulse: 0.5 and 1.0 s. Data are presented as boxplots, where the box indicates the 25th and 75th percentiles, the thin line within the box marks the median, and the whiskers represent the maximum and minimum values, showing all data. ** <span class="html-italic">p</span> &lt; 0.01; Mann–Whitney U test. Data are from 2 independent experiments with 4–5 adult wild-type short-fin zebrafish in each experimental group in each experiment.</p>
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