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Search Results (891)

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19 pages, 3743 KiB  
Article
Influence of Spacing on the Retention Process of Cascade Permeable Dams for Upstream Sediment-Laden Flow
by Jian Liu, Hongwei Zhou, Longyang Pan, Niannian Li, Mingyang Wang, Xing Gao and Haoxiang Yang
Water 2025, 17(1), 95; https://doi.org/10.3390/w17010095 - 1 Jan 2025
Viewed by 371
Abstract
Permeable dams are an important means for river management and ecology protection. Reasonable dam spacing will help regulate sediment transport and reduce sediment load in lakes. Flume experiments were carried out to investigate the effects of hydrological sediment conditions and dam spacing on [...] Read more.
Permeable dams are an important means for river management and ecology protection. Reasonable dam spacing will help regulate sediment transport and reduce sediment load in lakes. Flume experiments were carried out to investigate the effects of hydrological sediment conditions and dam spacing on sediment retention performance and permeability of the cascade permeable dams. The experimental results show that the permeability coefficient of the 1# dam decreased by about 30–40% with a large rate during the initial experiment stage. The decrease amplitude in the permeability coefficient and rising rate of the water level in front of the 1# dam for a large dam spacing (D/L) are positively correlated with the flow rate. At D/L = 5, the water level difference of 1# dam at the end of the experiment was significantly higher than that of other spacing. The sediment mass retained by 1# dam accounts for about 41–65% of the total sediment mass retained, which is about twice that of 2# dam, and plays a major role in cascade permeable dams. A mathematical model for predicting the spatial-temporal sediment concentration inside 1# dam is proposed based on the seepage theory of porous media. The research results are of great guiding significance for the design of the dam parameters. Full article
(This article belongs to the Section Water Quality and Contamination)
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<p>Schematic diagram of the experimental setup (Unit: mm): (<b>a</b>) Side view of the 1# dam during the experiment; (<b>b</b>) Grain size of the gravel dam material and the quartz sand; (<b>c</b>) Top view of the sedimentary deposition pattern between 1# and 2# dam.</p>
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<p>Relative water level change curve in front of 1# dam under different working conditions.</p>
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<p>Water level difference before and after dam 1# under different working conditions.</p>
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<p>The change curve of the relative permeability coefficient of 1# dam under different working conditions.</p>
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<p>Mass percentage of retained sediment along the cascade permeable dam. Note: <span class="html-italic">D</span> rises from 100 cm to 500 mm for each set of working conditions, respectively.</p>
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<p>Total water volume and total experimental time for different working conditions.</p>
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<p>Sediment retention rate per unit time at different locations of the cascade permeable dam: (<b>a</b>) <span class="html-italic">Q</span> = 300 L/h; (<b>b</b>) <span class="html-italic">Q</span> = 500 L/h; (<b>c</b>) <span class="html-italic">Q</span> = 700 L/h.</p>
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<p>Equation calculated values and experimental values.</p>
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21 pages, 4407 KiB  
Article
Inferential Approach for Evaluating the Association Between Land Cover and Soil Carbon in Northern Ontario
by Rory Pittman, Baoxin Hu, Tyler Pittman, Kara L. Webster, Jiali Shang and Stephanie A. Nelson
Earth 2025, 6(1), 1; https://doi.org/10.3390/earth6010001 - 1 Jan 2025
Viewed by 410
Abstract
Resolving the status of soil carbon with land cover is critical for addressing the impacts of climate change arising from land cover conversion in boreal regions. However, many conventional inferential approaches inadequately gauge statistical significance for this issue, due to limited sample sizes [...] Read more.
Resolving the status of soil carbon with land cover is critical for addressing the impacts of climate change arising from land cover conversion in boreal regions. However, many conventional inferential approaches inadequately gauge statistical significance for this issue, due to limited sample sizes or skewness of soil properties. This study aimed to address this drawback by adopting inferential approaches suitable for smaller samples sizes, where normal distributions of soil properties were not assumed. A two-step inference process was proposed. The Kruskal–Wallis (KW) test was first employed to evaluate disparities amongst soil properties. Generalized estimating equations (GEEs) were then wielded for a more thorough analysis. The proposed method was applied to soil samples (n = 431) extracted within the southern transition zone of the boreal forest (49°–50° N, 80°40′–84° W) in northern Ontario, Canada. Sites representative of eight land cover types and seven dominant tree species were sampled, investigating the total carbon (C), carbon-to-nitrogen ratio (C:N), clay percentage, and bulk density (BD). The KW test analysis corroborated significance (p-values < 0.05) for median differences between soil properties across the cover types. GEEs supported refined robust statistical evidence of mean differences in soil C between specific tree species groupings and land covers, particularly for black spruce (Picea mariana) and wetlands. In addition to the proposed method, the results of this study provided application for the selection of appropriate predictors for C with digital soil mapping. Full article
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<p>The location of the study areas and sampling sites, with grid lines and coordinates marked for all subfigures and insets. Backgrounds within delineated study areas correspond to canopy height model (CHM) [m]. Lakes and rivers are displayed within all study areas, as well as outside of study areas with respect to the study region for subfigures denoting sampling sites. (<b>A</b>) Location reference for the study areas in northern Ontario, Canada. (<b>B</b>) Locations of the sampling sites within the Cochrane study area, with insets zoomed in on clusters of sampling sites. (<b>C</b>) Locations of the sampling sites within the Hearst, Gordon Cosens Forest (GCF), and Kapuskasing (within GCF) study areas, with insets zoomed in on clusters of sampling sites.</p>
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<p>Soil properties as discerned by dominant tree species at site (mean values and standard errors are depicted) for (<b>A</b>) total carbon (C), (<b>B</b>) carbon-to-nitrogen ratio (C:N), (<b>C</b>) clay component, and (<b>D</b>) bulk density (BD).</p>
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<p>Soil properties as discerned by dominant tree species at site, as summarized by land cover type (mean values and standard errors are depicted) for (<b>A</b>) total carbon (C), (<b>B</b>) carbon-to-nitrogen ratio (C:N), (<b>C</b>) clay component, and (<b>D</b>) bulk density (BD).</p>
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10 pages, 11524 KiB  
Article
Lombard Sculptures from Saint Sophia of Kijv at the Russian National Museum in Moscow
by Spiriti Andrea
Arts 2025, 14(1), 1; https://doi.org/10.3390/arts14010001 - 31 Dec 2024
Viewed by 292
Abstract
A group of Romanesque sculptures today at the Gosudarstvennyj Istoričeskij Muzej in Moscow, coming from the restoration of the Cathedral of Saint Sophia in Kijv, can be related to the commission of Vladimir II Monomak, Grand Prince of Kijv, cultural heir of both [...] Read more.
A group of Romanesque sculptures today at the Gosudarstvennyj Istoričeskij Muzej in Moscow, coming from the restoration of the Cathedral of Saint Sophia in Kijv, can be related to the commission of Vladimir II Monomak, Grand Prince of Kijv, cultural heir of both his great-grandfather, the grand prince Vladimir I (who had founded the church between 1011 and 1037), and of his grandfather, the Eastern Roman Emperor Constantine IX: It is argued here that, alongside the Byzantine mosaicists certainly present, the sculptures are the work of a group of artists from the Lombardy lakes (also known as Comacine masters), attested in central and eastern Europe through Bavaria, Bohemia, Poland and then arriving in Sweden, active in Kijv between 1113 and 1125. It is probable that their specific origin is from Valchiavenna. Full article
(This article belongs to the Special Issue Russia: Histories of Mobility)
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<p>Man’s head. Gosudarstvenny Istorichesky Muzei, Moscow.</p>
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<p>Metope with female head. Gosudarstvenny Istorichesky Muzei, Moscow.</p>
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<p>Harpy.Gosudarstvenny Istorichesky Muzei, Moscow.</p>
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<p>Bird and lion protome. Gosudarstvenny Istorichesky Muzei, Moscow.</p>
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<p>Capital with phytomorphic motifs. Gosudarstvenny Istorichesky Muzei, Moscow.</p>
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<p>Capital with phytomorphic and intertwining motifs. Gosudarstvenny Istorichesky Muzei, Moscow.</p>
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<p>Base with heads and weaving. Gosudarstvenny Istorichesky Muzei, Moscow.</p>
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19 pages, 12098 KiB  
Article
Divergent Responses of Grassland Productivity to Large-Scale Atmospheric Circulations Across Ecoregions on the Mongolian Plateau
by Cuicui Jiao, Xiaobo Yi, Ji Luo, Ying Wang, Yuanjie Deng and Xiao Guo
Atmosphere 2025, 16(1), 32; https://doi.org/10.3390/atmos16010032 - 30 Dec 2024
Viewed by 299
Abstract
The Mongolian Plateau grassland (MPG) is critical for ecological conservation and sustainability of regional pastoral economies. Aboveground net primary productivity (ANPP) is a key indicator of grassland health and function, which is highly sensitive to variabilities in large-scale atmospheric circulations, commonly referred to [...] Read more.
The Mongolian Plateau grassland (MPG) is critical for ecological conservation and sustainability of regional pastoral economies. Aboveground net primary productivity (ANPP) is a key indicator of grassland health and function, which is highly sensitive to variabilities in large-scale atmospheric circulations, commonly referred to as teleconnections (TCs). In this study, we analyzed the spatial and temporal variations of ANPP and their response to local meteorological and large-scale climatic variabilities across the MPG from 1982 to 2015. Our analysis indicated the following: (1) Throughout the entire study period, ANPP displayed an overall upward trend across nine ecoregions. In the Sayan montane steppe and Sayan alpine meadow ecoregions, ANPP displayed a distinct inflection point in the mid-1990s. In the Ordos Plateau arid steppe ecoregion, ANPP continuously increased without any inflection points. In the six other ecoregions, trends in ANPP exhibited two inflection points, one in the mid-1990s and one in the late-2000s. (2) Precipitation was the principal determinant of ANPP across the entire MPG. Temperature was a secondary yet important factor influencing ANPP variations in the Ordos Plateau arid steppe. Cloud cover affected ANPP in Sukhbaatar and central Dornod, Mongolia. (3) The Atlantic Multidecadal Oscillation affected ANPP by regulating temperature in the Ordos Plateau arid steppe ecoregion, whereas precipitation occurred in the other ecoregions. The Pacific/North America, North Atlantic Oscillation, East Atlantic/Western Russia, and Pacific Decadal Oscillation predominantly affected precipitation patterns in various ecoregions, indicating regional heterogeneities of the effects of TCs on ANPP fluctuations. When considering seasonal variances, winter TCs dominated ANPP variations in the Selenge–Orkhon forest steppe, Daurian forest steppe, and Khangai Mountains alpine meadow ecoregions. Autumn TCs, particularly the Pacific/North America and North Atlantic Oscillation, had a greater impact in arid regions like the Gobi Desert steppe and the Great Lakes Basin desert steppe ecoregions. This study’s findings will enhance the theoretical framework for examining the effects of TCs on grassland ecosystems. Full article
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<p>Location of study area (<b>a</b>) and distribution of ecoregions (<b>b</b>).</p>
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<p>The multiyear averaged ANPP of the nine ecoregions of the MPG during 1982−2015. The error bars show the SD (standard deviation) of ANPP. Different letters (a, b, c, etc.) denote significant differences in AGB at <span class="html-italic">p</span> &lt; 0.05 (LSD test).</p>
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<p>ANPP for the MPG and its temporal dynamics. (<b>a</b>) ANPP trend during the entire period: the blue line, the orange line, and the green line indicate the linear trend, EEMD secular trend, and interdecadal fluctuation (secular trend plus IMF3), respectively. (<b>b</b>) ANPP linear trends during three different subperiods. * and ** indicate that a regression equation was significant at the 0.05 level and the 0.01 level, respectively.</p>
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<p>ANPP change trends in the nine ecoregions over the entire period and three subperiods. (<b>a</b>) 1982−2015; (<b>b</b>) 1982−1993/1982−1995; (<b>c</b>) 1993−2007/1995−2006; (<b>d</b>) 2007−2015/2006−2015. *, **, and *** indicate that the linear trend is significant at the 0.05 level, the 0.01 level and the 0.0001 level, respectively.</p>
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<p>Spatial patterns of the partial correlations between ANPP variations and (<b>a</b>) annual precipitation (AP), (<b>b</b>) mean annual temperature (MAT), and (<b>c</b>) cloud cover (CC). (<b>d</b>) Spatial patterns of the combinations of the correlations between ANPP variations and meteorological variables.</p>
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<p>Teleconnections with the greatest correlation with ANPP temporal dynamics during 1982−2015 and relevant information. (<b>a</b>) The spatial pattern of the maximum correlation coefficients between ANPP and teleconnection indices. (<b>b</b>) The spatial pattern of teleconnections that can best represent ANPP temporal dynamics. (<b>c</b>) The spatial pattern of the optimal seasonal period for the dominant teleconnections. (<b>d</b>) Statistics of the fraction of the MPG dominated by each teleconnection and its corresponding optimal seasonal period.</p>
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<p>Area fractions of TCs significantly correlated with ANPP in (<b>a</b>) the Ordos Plateau arid steppe ecoregion, (<b>b</b>) the Mongolian–Manchurian arid steppe ecoregion, (<b>c</b>) the Daurian forest steppe ecoregion, (<b>d</b>) the Selenge–Orkhon forest steppe ecoregion, (<b>e</b>) the Khangai Mountains alpine meadow ecoregion, (<b>f</b>) the Sayan montane steppe ecoregion, (<b>g</b>) the Sayan alpine meadow ecoregion, (<b>h</b>) the Gobi Desert steppe ecoregion, and (<b>i</b>) the Great Lakes Basin desert steppe ecoregion.</p>
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<p>Spatial patterns of partial correlations between dominant teleconnection indices and (<b>a</b>) annual precipitation, (<b>b</b>) mean annual temperature, and (<b>c</b>) cloud cover. (<b>d</b>) Spatial patterns of combinations of correlations between meteorological variables and dominant teleconnection indices.</p>
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18 pages, 9579 KiB  
Article
Remote Sensing Identification of Harmful Algae in Ulansuhai Lake with Machine Learning
by Jianglong Cui, Xiaodie Zhang, Caili Du and Guowen Li
Water 2025, 17(1), 50; https://doi.org/10.3390/w17010050 - 28 Dec 2024
Viewed by 418
Abstract
Frequent algal blooms in lakes pose a serious threat to aquatic ecosystems. It is of great significance to quickly and accurately monitor the distribution of algae in lakes for the regulation of algal blooms. While remote sensing techniques and machine learning methods can [...] Read more.
Frequent algal blooms in lakes pose a serious threat to aquatic ecosystems. It is of great significance to quickly and accurately monitor the distribution of algae in lakes for the regulation of algal blooms. While remote sensing techniques and machine learning methods can be used in combination to identify algae and analyze their spatial and temporal distribution, these methods still face challenges in practical applications due to uncertainties in lake boundaries and imbalances between algae and non-algae. In order to overcome these difficulties, we studied the dynamic open water range of Ulansuhai Lake and used a non-equilibrium data processing method to identify its algae. We also performed a spatiotemporal analysis of the algal range over a long time series. The results show that (1) the spectral characteristics of Landsat 8 images are very suitable for algal identification based on remote sensing, especially in the random forest method, where the fourth band plays an important role. (2) Among various machine learning methods, the accuracy of the random forest method on the training set and validation set is more than 90%. This indicates that the random forest method is suitable for the long-term monitoring of algal blooms. This study provides scientific and technical support for the management of Ulansuhai Lake, which will be helpful in guiding future management and control work. Full article
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<p>Location of the study area.</p>
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<p>Huangtai algae field collection photos.</p>
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<p>Huangtai algae label after visual interpretation.</p>
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<p>Workflow.</p>
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<p>(<b>a</b>) Ulansuhai Lake waterbody and (<b>b</b>) remote sensing image after cutting.</p>
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<p>Huangtai algae vectorization results.</p>
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<p>Spectral dissimilarity between the taxonomic classes.</p>
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<p>Variation trend chart of the open water area of Ulansuhai Lake.</p>
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<p>Area proportion of Huangtai algae per month.</p>
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<p>Portion of the recognition results.</p>
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24 pages, 9347 KiB  
Article
RDAU-Net: A U-Shaped Semantic Segmentation Network for Buildings near Rivers and Lakes Based on a Fusion Approach
by Yipeng Wang, Dongmei Wang, Teng Xu, Yifan Shi, Wenguang Liang, Yihong Wang, George P. Petropoulos and Yansong Bao
Remote Sens. 2025, 17(1), 2; https://doi.org/10.3390/rs17010002 - 24 Dec 2024
Viewed by 308
Abstract
The encroachment of buildings into the waters of rivers and lakes can lead to increased safety hazards, but current semantic segmentation algorithms have difficulty accurately segmenting buildings in such environments. The specular reflection of the water and boats with similar features to the [...] Read more.
The encroachment of buildings into the waters of rivers and lakes can lead to increased safety hazards, but current semantic segmentation algorithms have difficulty accurately segmenting buildings in such environments. The specular reflection of the water and boats with similar features to the buildings in the environment can greatly affect the performance of the algorithm. Effectively eliminating their influence on the model and further improving the segmentation accuracy of buildings near water will be of great help to the management of river and lake waters. To address the above issues, the present study proposes the design of a U-shaped segmentation network of buildings called RDAU-Net that works through extraction and fuses a convolutional neural network and a transformer to segment buildings. First, we designed a residual dynamic short-cut down-sampling (RDSC) module to minimize the interference of complex building shapes and building scale differences on the segmentation results; second, we reduced the semantic and resolution gaps between multi-scale features using a multi-channel cross fusion transformer module (MCCT); finally, a double-feature channel-wise fusion attention (DCF) was designed to improve the model’s ability to depict building edge details and to reduce the influence of similar features on the model. Additionally, an HRI Building dataset was constructed, comprising water-edge buildings situated in a riverine and lacustrine regulatory context. This dataset encompasses a plethora of water-edge building sample scenarios, offering a comprehensive representation of the subject matter. The experimental results indicated that the statistical metrics achieved by RDAU-Net using the HRI and WHU Building datasets are better than those of others, and that it can effectively solve the building segmentation problems in the management of river and lake waters. Full article
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<p>The architecture of RDAU-Net.</p>
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<p>The running flow of dynamic convolution.</p>
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<p>The structure of RDSC module.</p>
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<p>The structure of the MCCT module.</p>
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<p>Multi-channel attention mechanism.</p>
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<p>The structure of the DCF module.</p>
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<p>Example of a typical sample of the HRI Building dataset.</p>
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<p>Results of ablation experiments on the HRI Building dataset. (<b>a</b>) Image. (<b>b</b>) Ground truth. (<b>c</b>) Baseline. (<b>d</b>) Baseline + RDSC. (<b>e</b>) Baseline + RDSC + MCCT. (<b>f</b>) Baseline + RDSC + MCCT + DCF.</p>
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<p>Results of ablation experiments on the WHU Building dataset. (<b>a</b>) Image. (<b>b</b>) Ground truth. (<b>c</b>) Baseline. (<b>d</b>) Baseline + RDSC. (<b>e</b>) Baseline + RDSC + MCCT. (<b>f</b>) Baseline + RDSC + MCCT + DCF.</p>
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<p>Visualization of the results of comparative experiments on the HRI Building dataset. (<b>a</b>) Image. (<b>b</b>) Ground truth. (<b>c</b>) FCN. (<b>d</b>) U-Net. (<b>e</b>) U-Net++. (<b>f</b>) Swin-UNet. (<b>g</b>) ACC-UNet. (<b>h</b>) CSC-UNet. (<b>i</b>) UCTransNet. (<b>j</b>) DTA-UNet. (<b>k</b>) RDAU-Net.</p>
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<p>Visualization of the results of comparative experiments on the WHU Building dataset. (<b>a</b>) Image. (<b>b</b>) Ground truth. (<b>c</b>) FCN. (<b>d</b>) U-Net. (<b>e</b>) U-Net++. (<b>f</b>) Swin-UNet. (<b>g</b>) ACC-UNet. (<b>h</b>) CSC-UNet. (<b>i</b>) UCTransNet. (<b>j</b>) DTA-UNet. (<b>k</b>) RDAU-Net.</p>
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15 pages, 7166 KiB  
Article
Algal Pigment Estimation Models to Assess Bloom Toxicity in a South American Lake
by Lien Rodríguez-López, David Francisco Bustos Usta, Lisandra Bravo Alvarez, Iongel Duran-Llacer, Luc Bourrel, Frederic Frappart, Rolando Cardenas and Roberto Urrutia
Water 2024, 16(24), 3708; https://doi.org/10.3390/w16243708 - 22 Dec 2024
Viewed by 708
Abstract
In this study, we build an empirical model to estimate pigments in the South American Lake Villarrica. We use data from Dirección General de Aguas de Chile during the period of 1989–2024 to analyze the behavior of limnological parameters and trophic condition in [...] Read more.
In this study, we build an empirical model to estimate pigments in the South American Lake Villarrica. We use data from Dirección General de Aguas de Chile during the period of 1989–2024 to analyze the behavior of limnological parameters and trophic condition in the lake. Four seasonal linear regression models were developed by us, using a set of water quality variables that explain the values of phycocyanin pigment in Lake Villarrica. In the first case, we related chlorophyll-a (Chl-a) to phycocyanin, expecting to find a direct relationship between both variables, but this was not fulfilled for all seasons of the year. In the second case, in addition to Chl-a, we included water temperature, since this parameter has a great influence on the algal photosynthesis process, and we obtained better results. We discovered a typical seasonal variability given by temperature fluctuations in Lake Villarrica, where in the spring, summer, and autumn seasons, conditions are favorable for algal blooms, while in winter, the natural seasonal conditions do not allow increases in algal productivity. For a third case, we included the turbidity variable along with the variables mentioned above and the statistical performance metrics of the models improved significantly, obtaining R2 values of up to 0.90 in the case of the model for the fall season and a mean squared error (MSE) of 0.04 µg/L. In the last case used, we added the variable dissolved organic matter (MOD), and the models showed a slight improvement in their performance. These models may be applicable to other lakes with harmful algal blooms in order to alert the community to the potential toxicity of these events. Full article
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<p>(<b>a</b>) South America continent, (<b>b</b>) Chile in Latin America, (<b>c</b>) Region de la Araucanía and the location of Lake Villarrica in the black box, (<b>d</b>) Lake Villarrica and seven sampling stations.</p>
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<p>Behavior of limnological parameters in Lake Villarrica during the period 2021–2024.</p>
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<p>Correlation matrix between the predictors (NTU, Temp, O_D, Chl-a, MOD) and the dependent variable (PC) (<a href="#sec2dot3-water-16-03708" class="html-sec">Section 2.3</a> and <a href="#sec2dot4-water-16-03708" class="html-sec">Section 2.4</a>).</p>
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<p>Model performance is evaluated through a time series plot of predicted vs. actual FCA values (left) and comparisons of R<sup>2</sup> scores (top right) and mean squared errors (bottom right) across different models.</p>
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<p>Phytoplankton community present in Lake Villarica and their abundance according to depth over 2021–2024.</p>
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<p>Abundance percent of algal species in Lake Villarica over 2021–2024.</p>
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25 pages, 1589 KiB  
Article
Succession and Driving Factors of Macrophytes During the Past 60 Years in Lake Erhai, China
by Wen Hu, Jianjian Jiang, Jie Li, Haitao Feng, Di Song and Jufen Nie
Water 2024, 16(24), 3645; https://doi.org/10.3390/w16243645 - 18 Dec 2024
Viewed by 326
Abstract
Macrophytes play a crucial role in maintaining the health of lake ecosystems. A thorough understanding of their long-term evolutionary processes and patterns is of great theoretical and practical significance for ecosystem restoration and mitigation of lake eutrophication. The succession process and driving factors [...] Read more.
Macrophytes play a crucial role in maintaining the health of lake ecosystems. A thorough understanding of their long-term evolutionary processes and patterns is of great theoretical and practical significance for ecosystem restoration and mitigation of lake eutrophication. The succession process and driving factors of macrophytes in the Lake Erhai aquatic ecosystem were systematically analyzed using the investigation of macrophytes, literature research, and classification. A survey conducted in July 2022 showed that the macrophyte community in Lake Erhai is seriously degraded, with species numbers notably lower than historical levels from a decade ago (2011). The distribution area declined by over 70% compared to its peak in the 1980s. Over the past 60 years, the macrophyte community of Lake Erhai has undergone successive processes, including expansion, peak, decline, and stabilization. The dominant populations gradually transitioned from being indicative of clean water to pollution-tolerant species. The driving factors of the macrophytes succession of Lake Erhai were the development of cascade hydropower projects on the Xi’er River and the increased outflow capacity of Lake Erhai; these have resulted in substantial fluctuations in water levels, the eutrophication of the lake, pollutant discharge exceeding Lake Erhai’s environmental capacity, and substantial climate change in the Lake Erhai basin. Our research provides important theoretical references for ecological restoration and management of early eutrophic lakes in China. Full article
(This article belongs to the Section Biodiversity and Functionality of Aquatic Ecosystems)
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<p>Location of the study area.</p>
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<p>Sampling points.</p>
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<p>Distribution map of the 2022’s survey on macrophytes in Lake Erhai.</p>
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<p>Comparison of frequency and biomass of macrophytes in Lake Erhai (2022).</p>
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<p>Change of macrophytes community structure composition of Lake Erhai in 1944–2022.</p>
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<p>Trend of the nutrient concentrations of Lake Erhai in the past 40 years. (<b>a</b>) chemical oxygen demand (COD<sub>Cr</sub>), (<b>b</b>) total phosphorus (TP), (<b>c</b>) total nitrogen (TN), (<b>d</b>) permanganate index (COD<sub>Mn</sub>), and (<b>e</b>) comprehensive trophic level index (<span class="html-italic">TLI</span>).</p>
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<p>Water temperature in Lake Erhai, temperature in Dali Prefecture, and precipitation in Dali Prefecture from 2006 to 2023.</p>
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17 pages, 3627 KiB  
Article
Comparative Assessments of New Hair-Straightening Cosmetic Formulations on Wavy Type 2 Hair
by Celso Martins Junior, Matheus Henrique Vieira, Érica Savassa Pinto Cacoci, Ursulandrea Sanches Abelan, Fernanda Daud Sarruf, Cibele Castro Lima and Chung Man Chin
Cosmetics 2024, 11(6), 222; https://doi.org/10.3390/cosmetics11060222 - 16 Dec 2024
Viewed by 853
Abstract
Hair straighteners are among the most technically complex products to be safely and effectively developed, and this challenge has increased even more with the higher incidence of resistant hair among consumers. This underscores the importance of studying new active ingredients, combinations and carrier [...] Read more.
Hair straighteners are among the most technically complex products to be safely and effectively developed, and this challenge has increased even more with the higher incidence of resistant hair among consumers. This underscores the importance of studying new active ingredients, combinations and carrier formulations to improve performance without compromising safety. In this research, we compared eight hair-straightening formulations with different active ingredients and/or concentrations to develop new, safer and more effective texture modifiers. Eight formulations were developed and compared with each other and to controls (virgin and bleached hair) regarding mechanical and thermal resistance, cuticle morphology, hair shine and fiber diameter. Results showed that all formulations were safe and effective at straightening hair. Specifically, 13.3% and 9.4% ammonium thioglycolate (G03 and G04) were more suitable for wavy and curly hair, 12.5% and 7.9% amino methyl propanol thioglycolate (G05 and G06) for finer or chemically processed hair, 5% and 4% sodium cysteamine (G07 and G08) for curly and tight curly hair to control volume, and 2% and 1% of a combination of ammonium thioglycolate with sodium thioglycolate (G09 and G10) for more resistant wavy and curly hair. Full article
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<p>Hair tresses after product application per group (in left to right sequence: from G01 to G10).</p>
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<p>Hair fiber average diameter per treatment group and time. G01 = virgin hair; G02 = bleached hair; G03 = ammonium thioglycolate 13.3%; G04 = ammonium thioglycolate 9.4%; G05 = AMP thioglycolate 12.5%; G06 = AMP thioglycolate 7.9%; G07 = sodium cysteamine 5%; G08 = sodium cysteamine 4%; G09 = combination of ammonium thioglycolate with sodium thioglycolate 2%; G10 = combination of ammonium thioglycolate with sodium thioglycolate 1%. Groups that do not share a letter are significantly different.</p>
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<p>Analyzed SEM images per group.</p>
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<p>Thermal analysis profiles of the hair samples per treatment group: (<b>a</b>) DSC; (<b>b</b>) TG/DTG.</p>
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<p>Mechanical resistance results per treatment—average maximum force values <sup>1</sup>. <sup>1</sup> Letters a, b, c and d represent grouping variables of ANOVA analysis with Tukey post-test. Averages that do not share a letter are statistically different.</p>
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<p>Fluorescence microscopy images of straightened bleached and virgin hair fibers.</p>
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<p>Hair gloss values (average and interval—BNT luster) per treatment group.</p>
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14 pages, 8958 KiB  
Article
Improved Detection of Great Lakes Water Quality Anomalies Using Remote Sensing
by Karl R. Bosse, Robert A. Shuchman, Michael J. Sayers, John Lekki and Roger Tokars
Water 2024, 16(24), 3602; https://doi.org/10.3390/w16243602 - 14 Dec 2024
Viewed by 467
Abstract
Due to their immense economic and recreational value, the monitoring of Great Lakes water quality is of utmost importance to the region. Historically, this has taken place through a combination of ship-based sampling, buoy measurements, and physical models. However, these approaches have spatial [...] Read more.
Due to their immense economic and recreational value, the monitoring of Great Lakes water quality is of utmost importance to the region. Historically, this has taken place through a combination of ship-based sampling, buoy measurements, and physical models. However, these approaches have spatial and temporal deficiencies which can be improved upon through satellite remote sensing. This study details a new approach for using long time series of satellite remote sensing data to identify historical and near real-time anomalies across a range of data products. Anomalies are traditionally detected as deviations from historical climatologies, typically assuming that there are no long-term trends in the historical data. However, if present, such trends could result in misclassifying ordinary events as anomalous or missing actual anomalies. The new anomaly detection method explicitly accounts for long-term trends and seasonal variability by first decomposing a 10-plus year data record of satellite remote sensing-derived Great Lakes water quality parameters into seasonal, trend, and remainder components. Anomalies were identified as differences between the observed water quality parameter from the model-derived expected value. Normalizing the anomalies to the mean and standard deviation of the full model remainders, the relative anomaly product can be used to compare deviations across parameters and regions. This approach can also be used to forecast the model into the future, allowing for the identification of anomalies in near real time. Multiple case studies are detailed, including examples of a harmful algal bloom in Lake Erie, a sediment plume in Saginaw Bay (Lake Huron), and a phytoplankton bloom in Lake Superior. This new approach would be best suited for use in a water quality dashboard, allowing users (e.g., water quality managers, the research community, and the public) to observe historical and near real-time anomalies. Full article
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<p>Map showing the study area, consisting of all five of the Laurentian Great Lakes.</p>
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<p>Workflow diagram showing the process of getting from individual VIIRS satellite images to 10-day aggregated STL decomposition products.</p>
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<p>Lake-wide average seasonal time series for Lake Erie CHL (panel (<b>A</b>)), Lake Huron SM (panel (<b>B</b>)), and Lake Superior PZD (panel (<b>C</b>)). Each panel shows the annual seasonal patterns as light gray lines, the 11-year mean as a black line, and the 11-year standard deviation in the gray window.</p>
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<p>Lake-wide average time series are shown for the three example parameters: Lake Erie CHL (panels (<b>A</b>–<b>C</b>)), Lake Huron SM (panels (<b>D</b>–<b>F</b>)), and Lake Superior PZD (panels (<b>G</b>–<b>I</b>)). The panels in the left column show the parameter value time series. The panels in the center column show the absolute anomaly (A) time series. The panels in the right column show the relative anomaly (rA) time series.</p>
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<p>Western Lake Erie harmful algal bloom case study from 22 September 2015. Panel (<b>A</b>) shows the VIIRS true color image, with panel (<b>B</b>) showing the CPA-A derived CHL for the image. Panel (<b>C</b>) shows the expected CHL for this date based on the STL decomposition. Panels (<b>D</b>,<b>E</b>) show the absolute and relative CHL anomaly maps, respectively.</p>
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<p>Saginaw Bay sediment plume case study from 21 May 2020. Panel (<b>A</b>) shows the VIIRS true color image, with panel (<b>B</b>) showing the CPA-A derived SM for the image. Panel (<b>C</b>) shows the expected SM for this date based on the STL decomposition. Panels (<b>D</b>,<b>E</b>) show the absolute and relative SM anomaly maps, respectively.</p>
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<p>Lake Superior CHL forecast case study from 2 August 2023. Panel (<b>A</b>) shows the VIIRS true color image, with panel (<b>B</b>) showing the CPA-A derived CHL for the image. Panel (<b>C</b>) shows the expected CHL for this date based on the STL decomposition and forecast. Panels (<b>D</b>,<b>E</b>) show the absolute and relative CHL anomaly maps, respectively.</p>
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20 pages, 18277 KiB  
Article
Observations of Optical Properties and Chlorophyll-a Concentration in Qiandao Lake Using Shipborne Lidar
by Xuan Sang, Zhihua Mao, Youzhi Li, Xianliang Zhang, Chang Han, Longwei Zhang and Haiqing Huang
Remote Sens. 2024, 16(24), 4663; https://doi.org/10.3390/rs16244663 - 13 Dec 2024
Viewed by 461
Abstract
Lidar technology is increasingly applied to the inversion of oceanic biological parameters and optical properties based on empirical and semi-empirical bio-optical models. However, these models cannot be directly applied to inland waters due to their complex composition, and research on the biological parameters [...] Read more.
Lidar technology is increasingly applied to the inversion of oceanic biological parameters and optical properties based on empirical and semi-empirical bio-optical models. However, these models cannot be directly applied to inland waters due to their complex composition, and research on the biological parameters and optical properties of inland waters remains limited. In this study, the Fernald method was employed to retrieve the vertical distribution of optical properties in Qiandao Lake for the first time using shipborne lidar data obtained in June 2019. By quantifying the depth-resolved optical contributions of biological components, the vertical distributions of chlorophyll-a concentration were mapped with greater precision. The lidar-estimated optical properties exhibited characteristic spatiotemporal distributions, which were closely related to water quality. At the inflow of Xin’an River, the attenuation and scattering coefficient showed a gradual increase with depth. At the north–south-oriented reservoir area and the outflow of Qiandao Lake, an apparently continuous subsurface layer with the maximum signal occurred at approximately 3.5 m. The vertical distributions of chlorophyll-a profiles were consistently classified as subsurface chlorophyll maxima, with the maximum value of chlorophyll-a concentration fluctuating between 4 and 12 μg/L. The subsurface phytoplankton layer was observed at water depths ranging from 1.5 to 3.5 m, with a thickness of 3 to 6 m. Furthermore, the influences of lidar ratio Sp(z) and reference value bbp(zm) were discussed as significant sources of inversion error in the Fernald method. These results indicate that lidar technology holds great potential for the long-term monitoring of lakes. Full article
(This article belongs to the Special Issue Oceanographic Lidar in the Study of Marine Systems)
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<p>Map of shipborne lidar measurement tracks and in situ measurement stations conducted on (<b>A</b>) 3–5 June 2019 and (<b>B</b>) 10–12 July 2020 in Qiandao Lake. All stations are divided into three groups based on our study. The red pentagram represents the location of the Qiandao Lake.</p>
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<p>An example of lidar data preprocessing. (<b>A</b>) Raw lidar data and (<b>B</b>) water column signal from smoothed data. The distance 0 m is the position of the water surface, the distance below 0 m is the atmosphere altitude, and the distance above 0 m is the water depth.</p>
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<p>The depth-varying lidar ratio <span class="html-italic">S</span><sub>p</sub>(<span class="html-italic">z</span>) of the six measurement stations (S1–S6).</p>
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<p>Results of regression analysis of (<b>A</b>) fractions at different depths and (<b>B</b>) lidar-estimated and in-situ-observed chlorophyll-a concentrations.</p>
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<p>Step-by-step example of a lidar inversion along the lidar track. Vertical profile distributions of (<b>A</b>) signal after preprocessing, (<b>B</b>) lidar attenuation coefficient, (<b>C</b>) lidar volume scattering function at a scattering angle of π rad, (<b>D</b>) particulate backscatter coefficient, and (<b>E</b>) chlorophyll-a concentration.</p>
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<p>Comparisons of vertical profiles between lidar-estimated and in-situ-observed <span class="html-italic">b</span><sub>bp</sub> values at (<b>A</b>) S1, (<b>B</b>) S2, (<b>C</b>) S3, (<b>D</b>) S4, (<b>E</b>) S5, and (<b>F</b>) S6. Yellow dots are lidar estimations and blue dots are in situ observations.</p>
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<p>Comparisons of vertical profiles between lidar-estimated and in-situ-observed <span class="html-italic">K</span><sub>d</sub> values at (<b>A</b>) S1, (<b>B</b>) S2, (<b>C</b>) S3, (<b>D</b>) S4, (<b>E</b>) S5, and (<b>F</b>) S6. Yellow dots are lidar estimations and blue dots are in situ observations.</p>
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<p>Scatter plots of regression analysis results between lidar-estimated and in-situ-observed (<b>A</b>) <span class="html-italic">b</span><sub>bp</sub> and (<b>B</b>) <span class="html-italic">K</span><sub>d</sub> profiles for stations 1–6. The solid line is the linear regression.</p>
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<p>Three-dimensional profile distributions of (<b>A</b>) diffusion attenuation coefficient, (<b>B</b>) lidar volume scattering function at a scattering angle of π rad, (<b>C</b>) particulate backscatter coefficient, and (<b>D</b>) chlorophyll-a concentration estimated from the lidar data obtained during the 2019 cruise in Qiandao Lake.</p>
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<p>The distributions of SCM maximum depth (Z<sub>scm</sub>), SCM thickness (T<sub>scm</sub>), and maximum value of chlorophyll-a concentration (Chl<sub>max</sub>) along (<b>A</b>) Track 1, (<b>B</b>) Track 2, (<b>C</b>) Track 3, and (<b>D</b>) Track 4. All lidar tracks are plotted from north to south.</p>
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<p>The influence of setting different <span class="html-italic">S</span><sub>p</sub>(z) values. (<b>A</b>,<b>B</b>) Inversion results of <span class="html-italic">b</span><sub>bp</sub> and <span class="html-italic">K</span><sub>d</sub> under constant and depth-varying lidar ratios. (<b>C</b>,<b>D</b>) <span class="html-italic">b</span><sub>bp</sub> and <span class="html-italic">K</span><sub>d</sub> values obtained by setting different values of the constant S<sub>p</sub>(z).</p>
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<p>Comparison between lidar-estimated particulate backscatter coefficient with (<b>A</b>) different <span class="html-italic">b</span><sub>bp</sub>(<span class="html-italic">z</span><sub>m</sub>) values and (<b>B</b>) different <span class="html-italic">z</span><sub>m</sub> values.</p>
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<p>(<b>A</b>) Simulated echo signals with different reflectance of the bottom (Rb) and (<b>B</b>) the inversed value of the particulate backscatter coefficient.</p>
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13 pages, 1893 KiB  
Article
Cellulose Nanofibers as Rheological Modifiers to Improve Biomass Slurry Processing and Fermentation
by Zachary Jamieson, Jordi Francis Clar and Troy Runge
Fermentation 2024, 10(12), 626; https://doi.org/10.3390/fermentation10120626 - 8 Dec 2024
Viewed by 546
Abstract
This study investigates the enhancement of biomass slurry processability through the addition of rheological modifiers, focusing on carboxymethyl cellulose (CMC) and TEMPO-mediated oxidized cellulose nanofibrils (TCNF). Three sets of experiments were conducted to assess the effects of these additives on slurry processing and [...] Read more.
This study investigates the enhancement of biomass slurry processability through the addition of rheological modifiers, focusing on carboxymethyl cellulose (CMC) and TEMPO-mediated oxidized cellulose nanofibrils (TCNF). Three sets of experiments were conducted to assess the effects of these additives on slurry processing and fermentation. Initial experiments evaluated the slurry extrudability, concluding that TCNF aids extrusion similarly to CMC. Subsequent experiments explored slurry viscosity reduction mechanisms, revealing that while CMC significantly reduced the viscosity, TCNF’s impact is negligible. Additionally, TCNF performed comparably to CMC in water retention tests across different conditions, which suggests that TCNF have potential as an effective additive for maintaining slurry fluidity at high solid concentrations through enhanced water retention. Lastly, both additives were investigated to ensure that they did not impact hydrolyzed biomass fermentation. The findings suggest that TCNF’s mechanisms differ from those of traditional water-soluble polymers like CMC, offering insights into novel approaches to improve the biomass processing efficiency and subsequent fermentation. Full article
(This article belongs to the Special Issue Lignocellulosic Biomass Valorization)
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<p>Biomass processing steps with nanocellulose to enable higher solids.</p>
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<p>Diagram of the assembly used for extrusion in the simple extrusion assay. On the left is a simple diagram of the cylinder used for the extrusion assessment. On the right, a 3D model cross-section shows the pipe and its mount. The hole in the bottom extrudes into a tray to examine the quality of the extrusion.</p>
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<p>The graph shows the measured torques of biomass slurries with additives. Error bars depict the 95% confidence interval.</p>
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<p>WRV vs. centrifugal acceleration with weighted average estimate. All data points have error bars denoting standard deviations, although some are not visible due to the point itself.</p>
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<p>WRV vs. solid concentration with weighted average estimate. All slurries were held at 4 wt% additive of dry solids. All data points have error bars denoting standard deviations, although some are not visible due to the point itself.</p>
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<p>WRV vs. additive concentration with weighted average estimate. The figure shows the two groups, with blue denoting TCNF and orange denoting CMC. All data points have error bars denoting standard deviations, although some are not visible due to the point itself.</p>
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<p>Hydrolyzed biomass liquor primary components. Error bars denote the 95% confidence interval, with letters indicating values that are statistically different. The figure shows the concentrations of the primary analytes in the hydrolysate of the aqueous-ammonia-pretreated and enzymatically hydrolyzed switchgrass with two pulping additives, CMC (orange) and TCNF (yellow) at 8 wt%, and with no additive (blue).</p>
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<p>Fermentation results with different processing additives. Error bars denote the 95% confidence interval, with letters indicating groups that are statistically different.</p>
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9 pages, 1422 KiB  
Proceeding Paper
Utilizing CYGNSS Data for Flood Monitoring and Analysis of Influencing Factors
by Yan Jia, Quan Liu, Dawei Zhu, Heng Yu, Yuting Jiang and Junjie Wang
Proceedings 2024, 110(1), 20; https://doi.org/10.3390/proceedings2024110020 - 5 Dec 2024
Viewed by 444
Abstract
Flood disasters are among the most severe natural calamities worldwide and typically occur in densely populated areas with abundant lakes and high rainfall. These disasters cause significant damage to the environment and human settlements. Therefore, accurately monitoring and understanding the occurrence and evolution [...] Read more.
Flood disasters are among the most severe natural calamities worldwide and typically occur in densely populated areas with abundant lakes and high rainfall. These disasters cause significant damage to the environment and human settlements. Therefore, accurately monitoring and understanding the occurrence and evolution of floods, as well as studying the influencing factors, is of great importance. This study employs CYGNSS satellite data from a constellation of small satellites equipped with reflective radar, which observe the Earth’s surface with high spatial and temporal resolution. Such systems effectively monitor the distribution of water bodies and hydrological processes on land surfaces. By collecting and analyzing CYGNSS data, we can map the distribution of water bodies during flood events to assess the extent and severity of the flooding. Additionally, this study examines various factors influencing flooding, including rainfall, land use, and topography. By compiling relevant meteorological, geographical, and hydrological data, we aim to develop a model that elucidates the impacts of these factors on the initiation and progression of floods. Ultimately, this research offers a comprehensive analysis based on CYGNSS data for monitoring floods and their influencing factors. The goal is to yield significant insights and explore the potential of using CYGNSS data in flood monitoring efforts. In the context of global climate change and the increasing frequency of flood disasters, these findings are expected to provide a crucial scientific basis for improving flood prevention and management strategies, thereby helping to mitigate losses and enhance our warning and disaster response capabilities. Full article
(This article belongs to the Proceedings of The 31st International Conference on Geoinformatics)
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<p>Location, DEM and meteorological station distribution of the study area.</p>
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<p>Trends of the three-day mean values for CYGNSS reflectivity and precipitation: (<b>a</b>) the CYGNSS reflectance and precipitation map for 2021; (<b>b</b>) the CYGNSS reflectance and precipitation map for 2022.</p>
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<p>Spatial distribution of CYGNSS reflectivity and precipitation correlation at different scales: (<b>a</b>) 3 km scale; (<b>b</b>) 9 km scale.</p>
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19 pages, 6957 KiB  
Article
Transgenerational Plasticity Enhances the Tolerance of Duckweed (Lemna minor) to Stress from Exudates of Microcystis aeruginosa
by Gengyun Li, Tiantian Zheng, Gang Wang, Qian Gu, Xuexiu Chang, Yu Qian, Xiao Xu, Yi Wang, Bo Li and Yupeng Geng
Int. J. Mol. Sci. 2024, 25(23), 13027; https://doi.org/10.3390/ijms252313027 - 4 Dec 2024
Viewed by 500
Abstract
Transgenerational plasticity (TGP) refers to the influence of ancestral environmental signals on offspring’s traits across generations. While evidence of TGP in plants is growing, its role in plant adaptation over successive generations remains unclear, particularly in floating plants facing fluctuating environments. Duckweed ( [...] Read more.
Transgenerational plasticity (TGP) refers to the influence of ancestral environmental signals on offspring’s traits across generations. While evidence of TGP in plants is growing, its role in plant adaptation over successive generations remains unclear, particularly in floating plants facing fluctuating environments. Duckweed (Lemna minor), a common ecological remediation material, often coexists with the harmful bloom-forming cyanobacterium Microcystis aeruginosa, which releases a highly toxic exudate mixture (MaE) during its growth. In this study, we investigate the TGP of duckweed and its adaptive role under stress from MaE during the bloom-forming process. We found that exposure to MaE induces significant phenotypic plasticity in duckweed, manifested by alterations in morphological, physiological, and transcriptomic profiles. Specifically, MaE exposure significantly affected duckweed, promoting growth at low concentrations but inhibiting it at high concentrations, affecting traits like biomass, frond number, total frond area, and photosynthetic efficiency. Additionally, the activities of antioxidant enzymes, together with the levels of proline, soluble sugars, and proteins, are elevated with increasing MaE concentrations. These plastic changes are largely retained through asexual reproductive cycles, persisting for several generations even under MaE-free conditions. We identified 619 genes that maintain a ‘transcriptional memory’, some of which correlate with the TGP-linked alterations in morphological and physiological traits in response to MaE stress. Notably, progeny from MaE-exposed lineages demonstrate enhanced fitness when re-exposed to MaE. These results enhance our comprehension of the adaptive significance of TGP in plants and suggest feasible approaches for utilizing duckweed’s TGP in the bioremediation of detrimental algal blooms. Full article
(This article belongs to the Special Issue Omics Studies for Stress Responses and Adaptive Evolution in Plants)
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<p>Experimental design. (<b>A</b>) Acquisition of different MaE concentrations. CK (0 cells/mL), low (4 × 10<sup>4</sup> cells/mL), medium (4 × 10<sup>5</sup> cells/mL), and S (4 × 10<sup>6</sup> cells/mL). (<b>B</b>) Duckweed exposure to various MaE concentrations in P1, followed by two additional cultivation cycles in P2 and P3. In the P4 stage, descendants originating from P1-CK and P1-S were cultivated separately under CK and S conditions.</p>
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<p>Variation of duckweed traits in response to different concentrations of MaE in P1. (<b>A</b>,<b>B</b>) Biomass of fresh leaf (<b>A</b>) and dry leaf (<b>B</b>). (<b>C</b>) Number of leaves. (<b>D</b>) Total area of leaf. (<b>E</b>) Length of root. (<b>F</b>) F0. (<b>G</b>) Fm. (<b>H</b>) Fv/Fm. (<b>I</b>,<b>J</b>) Content of Chl a (<b>I</b>) and Chl b (<b>J</b>). (<b>K</b>–<b>M</b>) Activity of SOD (<b>K</b>), POD (<b>L</b>), and APX (<b>M</b>). (<b>N</b>) Content of proline. (<b>O</b>) Content of soluble sugar. (<b>P</b>) Content of soluble protein. Different letters indicate significant differences among groups determined using Fisher’s LSD test.</p>
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<p>The trait variation in P1-P3 stage. (<b>A</b>) Number of leaves. (<b>B</b>) Total area of leaf. (<b>C</b>) Length of root. (<b>D</b>,<b>E</b>) Content of Chl a (<b>D</b>) and Chl b (<b>E</b>). (<b>F</b>) F0. (<b>G</b>) Fm. (<b>H</b>) Fv/Fm. (<b>I</b>,<b>K</b>) Content of proline (<b>I</b>), soluble sugar (<b>J</b>), and soluble protein (<b>K</b>). (<b>L</b>–<b>O</b>) The activity of antioxidant enzymes, including APX (<b>L</b>), CAT (<b>M</b>), SOD (<b>N</b>), and POD (<b>O</b>). The red line and blue line represent the parent environments of P1-S and P1-CK, respectively. Different letters indicate significant differences among groups determined using Fisher’s LSD test.</p>
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<p>Reaction norm of trait expression in P4 individuals exposed to CK (0 cells/mL) and S (4 × 10<sup>6</sup> cells/mL). The blue and red lines indicate the offspring of P1-CK and P1-S, respectively. (<b>A</b>,<b>B</b>) Biomass of fresh leaf (<b>A</b>) and dry leaf (<b>B</b>). (<b>C</b>) Number of leaves. (<b>D</b>) Total area of leaf. (<b>E</b>,<b>F</b>) Content of Chl a (<b>E</b>) and Chl b (<b>F</b>). (<b>G</b>) F0. (<b>H</b>) Fm. (<b>I</b>) Fv/Fm. (<b>J</b>–<b>L</b>) The activity of antioxidant enzymes, including APX (<b>J</b>), POD (<b>K</b>), and SOD (<b>L</b>). (<b>M</b>–<b>O</b>) Content of proline (<b>M</b>), soluble sugar (<b>N</b>), and soluble protein (<b>O</b>). Different letters indicate significant differences among groups determined using Fisher’s LSD test.</p>
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<p>Expression and function of differentially expressed genes relative to CK in each period. (<b>A</b>) Number of upregulated and downregulated genes in P1 (S vs. CK), P2 (P1S–P2CK vs. CK), P3 (P1S–P3CK vs. CK), and P4 (P1S–P4S vs. CK). (<b>B</b>) Heatmap of differentially expressed genes. The color represents log2 fold change values; negative values (blue) represent downregulation, and positive values (red) represent upregulation. (<b>C</b>–<b>F</b>) Biological processes of differentially expressed genes of P1 (<b>C</b>), P2 (<b>D</b>), P3 (<b>E</b>), and P4 (<b>F</b>). The color and size were adjusted to <span class="html-italic">p</span>-value and log10 <span class="html-italic">p</span>-value, respectively.</p>
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<p>Identification and characterization of MaE-responsive TMGs. (<b>A</b>,<b>B</b>) Identification of downregulated (<b>A</b>) and upregulated (<b>B</b>) TMGs. The intersection of S vs. CK, P1S–P4S vs. CK, and P1S–P2CK vs. CK was identified as TMG2. The intersection of TMG2 and P1S–P4S vs. CK was identified as TMG3. (<b>C</b>,<b>D</b>) Expression and biological processes of TMG2 (<b>C</b>) and TMG3 (<b>D</b>). For the heatmap, color was adjusted to log2 fold change of comparison pairs. For GO map results, the color and size were adjusted to <span class="html-italic">p</span>-value and log10 <span class="html-italic">p</span>-value, respectively.</p>
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<p>Identification and characterization of traits related gene co-expression modules. (<b>A</b>) Module–trait associations. (<b>B</b>) The number of genes involved in different biological processes within each trait-related module. The color intensity represents the magnitude of log2–transformed values. (<b>C</b>) A subnetwork composed of weaken trait-related modules (turquoise, light green, magenta, green, and purple) and strengthen trait-related modules (blue, yellow, red, salmon, and light cyan). (<b>D</b>) More TMGs were found in strengthen and weaken trait-related modules than in other modules.</p>
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<p>Subnetworks composed of gene nodes related to TGP traits and their immediate network neighbors. (<b>A</b>,<b>B</b>) Network of developmental growth-related genes (<b>A</b>). Some developmental growth-related genes exhibit a similar expression pattern to weaken traits, which means they are initially suppressed by MaE at the P1 stage, gradually recover to normal expression levels in the P2–P3 stages, and are less suppressed at the P4 stage when re-exposed to MaE compared to the P1 stage (<b>B</b>). (<b>C</b>,<b>D</b>) Network of oxidative responsive genes (<b>C</b>). Among them, some exhibit a similar expression trend to SOD, where they are induced by MaE at the P1 stage, gradually recover during the P2–P3 stages, and are upregulated to a level higher than that at the P1 stage when re-exposed to MaE at the P4 stage (<b>D</b>). For networks, transcriptional factors and some key genes were marked. TMGs are represented by triangle, and modules that the gene belongs to are represented by different colors. Node size is positively correlated with the degree of connectivity. In panels B and D, the blue lines represent the expression trends of individual genes, while the red lines represent the overall expression trend.</p>
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<p>(<b>A</b>–<b>H</b>) Validation of expression pattern of eight TMGs via qRT-PCR. The blue and red bars represent the FPKM values and relative expression values from RNA-seq and the qRT-PCR results, respectively. For the qRT-PCR results, relative expression quantification was carried out using the 2<sup>−ΔΔCt</sup> method, with <span class="html-italic">UBQ10-2</span> as the internal reference gene. Error bars represent the mean ± SE (n = 3).</p>
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42 pages, 3923 KiB  
Review
Environmental Exposure to Per- and Polyfluorylalkyl Substances (PFASs) and Reproductive Outcomes in the General Population: A Systematic Review of Epidemiological Studies
by Alex Haimbaugh, Danielle N. Meyer, Mackenzie L. Connell, Jessica Blount-Pacheco, Dienye Tolofari, Gabrielle Gonzalez, Dayita Banerjee, John Norton, Carol J. Miller and Tracie R. Baker
Int. J. Environ. Res. Public Health 2024, 21(12), 1615; https://doi.org/10.3390/ijerph21121615 - 2 Dec 2024
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Abstract
This Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) systematic review synthesized effects of background levels of per- and polyfluorylalkyl substance (PFAS) levels on reproductive health outcomes in the general public: fertility, preterm birth, miscarriage, ovarian health, menstruation, menopause, sperm health, and [...] Read more.
This Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) systematic review synthesized effects of background levels of per- and polyfluorylalkyl substance (PFAS) levels on reproductive health outcomes in the general public: fertility, preterm birth, miscarriage, ovarian health, menstruation, menopause, sperm health, and in utero fetal growth. The inclusion criteria included original research (or primary) studies, human subjects, and investigation of outcomes of interest following non-occupational exposures. It drew from four databases (Web of Science, PubMed, Embase and Health and Environmental Research Online (HERO)) using a standardized search string for all studies published between 1 January 2017 and 13 April 2022. Risk of bias was assessed by two independent reviewers. Data were extracted and reviewed by multiple reviewers. Each study was summarized under its outcome in terms of methodology and results and placed in context, with recommendations for future research. Of 1712 records identified, 30 were eligible, with a total of 27,901 participants (33 datasets, as three studies included multiple outcomes). There was no effect of background levels of PFAS on fertility. There were weakly to moderately increased odds of preterm birth with higher perfluorooctane sulfonic acid (PFOS) levels; the same for miscarriage with perfluorooctanoic acid (PFOA) levels. There was limited yet suggestive evidence for a link between PFAS and early menopause and primary ovarian insufficiency; menstrual cycle characteristics were inconsistent. PFAS moderately increased odds of PCOS- and endometriosis-related infertility, respectively. Sperm motility and DNA health were moderately impaired by multiple PFAS. Fetal growth findings were inconsistent. This review may be used to inform forthcoming drinking water standards and policy initiatives regarding PFAS compounds and drinking water. Future reviews would benefit from more recent studies. Larger studies in these areas are warranted. Future studies should plan large cohorts and open access data availability to capture small effects and serve the public. Funding: Great Lakes Water Authority (Detroit, MI), the Erb Family Foundation through Healthy Urban Waters at Wayne State University (Detroit, MI), and Wayne State University CLEAR Superfund Research (NIH P42ES030991). Full article
(This article belongs to the Section Environmental Health)
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<p>PRISMA flow diagram.</p>
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<p>Pie charts depicting study characteristics. Percentages are rounded to nearest whole number and may not add up to 100%. (<b>a</b>) Number of studies included for each outcome. Some studies measured multiple outcomes. (<b>b</b>) Media type used in studies. Some studies used multiple media. (<b>c</b>) Study design type. (<b>d</b>) Open access status. (<b>e</b>) Region of studies. Scandinavia includes Sweden, Norway, Faroe Islands, and Denmark.</p>
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<p>(<b>a</b>) Participants in each outcome. (<b>b</b>) Average risk of bias scores in each outcome stratified by cohort or cross-sectional studies (left) or case–control (right). Minimum points to be included in the review for cohort/cross-sectional was 7; maximum achievable was 10. Minimum points to be included in the review for case–control was 10; maximum achievable was 14. (<b>c</b>) Median PFAS levels (ng/mL) in each study in maternal/paternal blood, serum, or plasma. Levels reported from controls only in case–control studies. Multi-region studies denoted with (S) for Sweden, (N) for Norway, (Y) for Yantai, and (B) for Beijing. Meng et al., 2018 [<a href="#B58-ijerph-21-01615" class="html-bibr">58</a>]. Sagiv et al., 2017 [<a href="#B59-ijerph-21-01615" class="html-bibr">59</a>]. Liew et al., 2020 [<a href="#B60-ijerph-21-01615" class="html-bibr">60</a>]. Petersen et al., 2018 [<a href="#B61-ijerph-21-01615" class="html-bibr">61</a>]. Ding et al., 2020 [<a href="#B62-ijerph-21-01615" class="html-bibr">62</a>]. Lauritzen et al., 2017 [<a href="#B63-ijerph-21-01615" class="html-bibr">63</a>]. Kalloo et al., 2020 [<a href="#B64-ijerph-21-01615" class="html-bibr">64</a>]. Singer et al., 2018 [<a href="#B65-ijerph-21-01615" class="html-bibr">65</a>]. Zhou et al., 2017 [<a href="#B66-ijerph-21-01615" class="html-bibr">66</a>]. Song et al., 2018 [<a href="#B67-ijerph-21-01615" class="html-bibr">67</a>]. Huo et al., 2020 [<a href="#B68-ijerph-21-01615" class="html-bibr">68</a>]. Pan et al., 2019 [<a href="#B69-ijerph-21-01615" class="html-bibr">69</a>]. Chu et al., 2020 [<a href="#B70-ijerph-21-01615" class="html-bibr">70</a>]. Wang et al., 2021 [<a href="#B71-ijerph-21-01615" class="html-bibr">71</a>]. Wang et al., 2017 [<a href="#B72-ijerph-21-01615" class="html-bibr">72</a>]. Costa et al., 2019 [<a href="#B73-ijerph-21-01615" class="html-bibr">73</a>]. Manzano-Salgado et al., 2017 [<a href="#B74-ijerph-21-01615" class="html-bibr">74</a>]. Zhang et al., 2018 [<a href="#B75-ijerph-21-01615" class="html-bibr">75</a>]. Wikström et al., 2021 [<a href="#B76-ijerph-21-01615" class="html-bibr">76</a>]. Ouidir et al., 2020 [<a href="#B77-ijerph-21-01615" class="html-bibr">77</a>]. Wang et al., 2021 [<a href="#B71-ijerph-21-01615" class="html-bibr">71</a>]. Wise et al., 2022 [<a href="#B78-ijerph-21-01615" class="html-bibr">78</a>]. Wang et al., 2019 [<a href="#B79-ijerph-21-01615" class="html-bibr">79</a>]. Bjorvang et al., 2022 [<a href="#B80-ijerph-21-01615" class="html-bibr">80</a>]. Heffernan et al., 2018 [<a href="#B81-ijerph-21-01615" class="html-bibr">81</a>]. Bjorvang et al., 2021 [<a href="#B82-ijerph-21-01615" class="html-bibr">82</a>]. Eick &amp; Hom Thepaksorn et al., 2020 [<a href="#B83-ijerph-21-01615" class="html-bibr">83</a>]. Liu et al., 2020 [<a href="#B84-ijerph-21-01615" class="html-bibr">84</a>]. Asterisk denotes the concentration of controls in case-control studies, if overall median is not reported.</p>
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<p>Forest plot of odds and risk ratios for preterm birth with increasing PFAS levels from four studies. LNE: line of no effect. PFNA: perfluorononanoic acid. PFHxS: perfluorohexane sulfonic acid. PFOS: perfluorooctane sulfonic acid. PFOA: perflurooctanoic acid. Yang et al., 2022 [<a href="#B108-ijerph-21-01615" class="html-bibr">108</a>]. Sagiv et al., 2018 [<a href="#B59-ijerph-21-01615" class="html-bibr">59</a>]. Manzano-Salgado et al., 2017 [<a href="#B74-ijerph-21-01615" class="html-bibr">74</a>]. Eick &amp; Hom Thepaksorn et al., 2020 [<a href="#B83-ijerph-21-01615" class="html-bibr">83</a>]. Liu et al., 2020 [<a href="#B84-ijerph-21-01615" class="html-bibr">84</a>]. Chu et al., 2020 [<a href="#B70-ijerph-21-01615" class="html-bibr">70</a>].</p>
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<p>Forest plot of miscarriage odds and risk ratios with increasing PFAS levels from three studies. LNE: line of no effect. PFOS: perfluorooctane sulfonic acid. PFOA: perflurooctanoic acid. PFNA: perfluorononanoic acid. PFHxS: perfluorohexane sulfonic acid. PFDA: perfluorodecanoic acid. Wang et al. (2021) results are from Beijing and Yantai sites combined. Wang et al., 2021 [<a href="#B71-ijerph-21-01615" class="html-bibr">71</a>]. Wikström et al., 2021 [<a href="#B76-ijerph-21-01615" class="html-bibr">76</a>]. Liew et al., 2020 [<a href="#B60-ijerph-21-01615" class="html-bibr">60</a>].</p>
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<p>Forest plot of odds and risk ratios of ovarian health effects (top: endometriosis; bottom: PCOS-related infertility) with increasing PFAS levels from two studies. PFHpA: perfluoroheptanoic acid. PFBS: perfluorobutanesulfonic acid. PFDoA: perfluorododecanoic acid. PFHxS: perfluorohexane sulfonic acid. PFUA: perfluoroundecanoic acid. PFDA: perfluorodecanoic acid. PFNA: perfluorononanoic acid. PFOS: perfluorooctanesulfonic acid. PFOA: perfluorooctanoic acid. Wang et al., 2019 [<a href="#B184-ijerph-21-01615" class="html-bibr">184</a>]. Wang et al., 2017 [<a href="#B72-ijerph-21-01615" class="html-bibr">72</a>].</p>
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<p>Forest plot of beta coefficients for sperm health effects with increasing PFAS levels in semen from two studies. Top panel: DNA stability (decreasing); DNA fragmentation index. Bottom panel: sperm motility (% motile sperm). PFDA: perfluorodecanoic acid. PFUnDA: perfluoroundecanoic acid. 6:2 Cl-PFESA: 6:2 chlorinated polyfluorinated ether sulfonate. PFNA: perfluorononanoic acid. PFOS: perfluorooctanesulfonic acid. PFOA: perfluorooctanoic acid. Pan et al., 2019 [<a href="#B69-ijerph-21-01615" class="html-bibr">69</a>]. Petersen et al., 2018 [<a href="#B61-ijerph-21-01615" class="html-bibr">61</a>].</p>
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<p>For each PFAS, median level (ng/mL) across ethnicities (concentrations are compared by column, not row). Color darkens with increasing median concentration. Me-FOSAA: N-methylperfluorooctane sulfonamidoacetic acid. PFDA: perfluorodecanoic acid. PFDoDA: perfluorododecanoic acid. PFHxS: perfluorohexane sulfonate. PFNA: perfluorononanoic acid. PFOA: perfluorooctanoic acid. PFOS: perfluorooctane sulfonate. PFUnDA: perfluoroundecanoic acid.</p>
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<p>Heatmap of beta-values at FDR &lt; 0.05 for measures of fetal growth, broken down by group. Warmer colors (orange, red) indicate lower values, yellow indicates mid-range values, and green indicates higher values. AC: abdominal circumference, FL: femur length, EFW: estimated fetal weight, BD: biparietal diameter. Head circumference not included (not significant for any ethnicity). Me-FOSAA: N-methylperfluorooctane sulfonamidoacetic acid. PFDA: perfluorodecanoic acid. PFDoDA: perfluorododecanoic acid. PFHxS: perfluorohexane sulfonate. PFNA: perfluorononanoic acid. PFOA: perfluorooctanoic acid. PFOS: perfluorooctane sulfonate. PFUnDA: perfluoroundecanoic acid.</p>
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