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Keywords = Burkina Faso

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18 pages, 3626 KiB  
Article
Effect of Organic Nitrogen Supply on the Kinetics and Quality of Anaerobic Digestion of Less Nitrogenous Substrates: Case of Anaerobic Co-Digestion (AcoD) of Cassava Effluent and Chicken Droppings as a Nitrogen Source
by Haro Kayaba, Nourou Abdel Anziph Sergel Khalid, Sandwidi Sayouba, Compaore Abdoulaye, Palm Sie Auguste, Sessouma Oumou, Ouedraogo Ibrahim Kourita, Sinon Souleymane, Tubreoumya Guy Christian, Bere Antoine, Daho Tizane and Sanogo Oumar
Fuels 2025, 6(1), 2; https://doi.org/10.3390/fuels6010002 - 30 Dec 2024
Viewed by 533
Abstract
This study aims to explore anaerobic co-digestion (AcoD) of cassava (EUM) and poultry (FP) effluents using one inoculum/substrate ratio (30%) and three EUM vs. FP substrate composition ratios (25:75, 50:50, and 75:25). The AcoD process was therefore designed for 20 L batch digesters, [...] Read more.
This study aims to explore anaerobic co-digestion (AcoD) of cassava (EUM) and poultry (FP) effluents using one inoculum/substrate ratio (30%) and three EUM vs. FP substrate composition ratios (25:75, 50:50, and 75:25). The AcoD process was therefore designed for 20 L batch digesters, under mesophilic conditions, with less than 5% total solids for 66 days. The results showed that EUMs were highly resistant to degradation, while FPs were the most easily degradable. Kinetic analysis indicated specific organic matter (MO) reduction rates of 0.28% per day for EUM and 0.76% per day for FP. EUM alone produced 45.47 mL/g MO, while the 50:50 substrate produced 1184.60 mL/g MOV. The main factors contributing to EUM inefficiency were the inability to tame acidic conditions and the accumulation of volatile fatty acids. AcoD produced 23 to 50 times more methane than EUM alone, 2 to 5 times more than FP alone, and 2 to 4 times more than inoculum. As a result, the AcoD of both types of waste had a qualitative and quantitative effect on biogas production. CH4 content increased from around 2 to 75%, depending on the amount of organic nitrogen added. The addition of nitrogen by AcoD, even under mesophilic conditions, improves the kinetics and quality of anaerobic digestion of low-nitrogen substrates. Its impact on thermophilic and psychrophilic conditions needs to be verified. Full article
(This article belongs to the Special Issue Biomass Conversion to Biofuels)
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<p>Schematic diagram of AD system: 1: cassava effluent; 2: chicken droppings; 3: 25%EUM + 75%FP; 4: 50%EUM + 50%FP; 5: 75% EUM + 25% FP and 6: inoculum.</p>
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<p>Daily methane production from various reactors.</p>
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<p>Kinetic evolution of anaerobic digestion from different reactors: (<b>a</b>): chicken droppings; (<b>b</b>): cassava effluent; (<b>c</b>): inoculum; (<b>d</b>): 75% EUM + 25% FP; (<b>e</b>): 50%EUM + 50%FP; and (<b>f</b>): 25%EUM + 75%FP.</p>
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<p>Kinetic evolution of anaerobic digestion from different reactors: (<b>a</b>): chicken droppings; (<b>b</b>): cassava effluent; (<b>c</b>): inoculum; (<b>d</b>): 75% EUM + 25% FP; (<b>e</b>): 50%EUM + 50%FP; and (<b>f</b>): 25%EUM + 75%FP.</p>
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<p>Kinetic evolution of anaerobic digestion from different reactors: (<b>a</b>): chicken droppings; (<b>b</b>): cassava effluent; (<b>c</b>): inoculum; (<b>d</b>): 75% EUM + 25% FP; (<b>e</b>): 50%EUM + 50%FP; and (<b>f</b>): 25%EUM + 75%FP.</p>
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<p>Cumulated methane production from various reactors.</p>
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<p>Effect of organic nitrogen addition on methane production in different reactors: FP: poultry effluents and EUM: cassava effluents.</p>
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10 pages, 573 KiB  
Article
Assessing the Acceptability and Feasibility of Five Cycles of Seasonal Malaria Chemoprevention in Côte d’Ivoire
by Orphée Kangah, Issaka Zongo, Alassane Haro and William Yavo
Trop. Med. Infect. Dis. 2025, 10(1), 10; https://doi.org/10.3390/tropicalmed10010010 - 30 Dec 2024
Viewed by 299
Abstract
Seasonal malaria chemoprevention (SMC) is a strategy recommended by the World Health Organization for children aged 3–59 months in the Sahel and sub-Sahel regions where malaria transmission is seasonal. In Côte d’Ivoire, malaria remains a high priority and accounts for the majority of [...] Read more.
Seasonal malaria chemoprevention (SMC) is a strategy recommended by the World Health Organization for children aged 3–59 months in the Sahel and sub-Sahel regions where malaria transmission is seasonal. In Côte d’Ivoire, malaria remains a high priority and accounts for the majority of consultations and deaths in children under five. The recent revision of the criteria for the introduction of seasonal malaria chemoprevention has made the north of Côte d’Ivoire, where malaria transmission is seasonal, eligible for the SMC. We conducted a pilot study in this part of the country to assess the acceptability and feasibility of five cycles of SMC in 1701 children. Seasonal malaria chemoprevention with sulfadoxine–pyrimethamine + amodiaquine (SP + AQ) was administered monthly to eligible children over five months. A qualitative approach and quantitative surveys were used to assess the strategy acceptability and feasibility in the study area. Overall, there was a positive perception, attitude, and adherence towards the seasonal malaria chemoprevention in this study area. Full article
(This article belongs to the Section Vector-Borne Diseases)
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<p>Comparison of SMC coverage by cycle.</p>
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17 pages, 9272 KiB  
Review
An Overview on the Manufacture and Properties of Clay-Based Porous Ceramics for Water Filtration
by Iffat Qoudsiyyah Maury Njoya, Gisèle Laure Lecomte-Nana, Kassoum Barry, Dayirou Njoya, Youssef El Hafiane and Claire Peyratout
Ceramics 2025, 8(1), 3; https://doi.org/10.3390/ceramics8010003 - 30 Dec 2024
Viewed by 300
Abstract
This study explores the different techniques used to manufacture porous clay-based ceramics, examining their properties such as porosity, strength, permeability and filtration efficiency. Different techniques are discussed in this review, with additive manufacturing being one of the most innovative techniques for manufacturing porous [...] Read more.
This study explores the different techniques used to manufacture porous clay-based ceramics, examining their properties such as porosity, strength, permeability and filtration efficiency. Different techniques are discussed in this review, with additive manufacturing being one of the most innovative techniques for manufacturing porous ceramics. Porous ceramics have their applications in numerous domains. Such ceramic filters have the advantages of retaining heavy materials, suspended particles, bacteria, viruses and, water turbidity. Thus, the choice of the technique and propriety is a crucial step in obtaining a porous ceramic with the best performance. Barry et al. prepared porous phyllosilicate-based ceramics by freeze-tape casting on four samples and obtained porosity values in the range of 67–79% and diametrical compressive strength in the range of 3–7 MPa. Manni et al. prepared porous red ceramics from Moroccan clay and coffee waste (10, 20 and 30 wt.%) via uniaxial pressing and sintering at 1150 °C. They obtained porosities ranging from 30.2 to 63.8% and flexural strength values from 1.8 to 19.5 MPa. Medri et al. prepared ZrB2-based porous bodies with the use of sponges and polyurethane foams as templates via the replica method and obtained high porosity over 80% and compressive strength up to 4.8 MPa. The use of clay and peanut shell mixtures was used in preparing porous silicate ceramics after unidirectional pressing and sintering at 1100 °C. These samples included 25 mass% of peanut shells, and exhibited porosity in the range of 40 to 60% and diametrical compressive strength in the range of 1–6 MPa. Such properties are suitable for domestic use of these types of clay-based ceramic filters. Moreover, the permeability values and removal of some pollutants, like arsenic, have been satisfactory for the first set of samples. Full article
(This article belongs to the Special Issue Innovative Manufacturing Processes of Silicate Materials)
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<p>Illustrating the use of water from a well, river and drill for drinking purposes.</p>
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<p>Applications of porous ceramics.</p>
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<p>Evolution of the number of research articles published on “porous ceramics” according to the Scopus database since 2010 [<a href="#B3-ceramics-08-00003" class="html-bibr">3</a>].</p>
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<p>Some shaping techniques for ceramic production [<a href="#B22-ceramics-08-00003" class="html-bibr">22</a>,<a href="#B23-ceramics-08-00003" class="html-bibr">23</a>].</p>
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<p>Main steps in the manufacturing of ceramics: direct foaming (<b>a</b>), replication technique (<b>b</b>), partial sintering process (<b>c</b>) and methods using pore-forming agents mixed with ceramic powders (<b>d</b>).</p>
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<p>SEM images of Halloysite (<b>a</b>) and Kaolin (<b>b</b>) based porous ceramics (shaped by uniaxial pressing and sintered at 1100 °C).</p>
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<p>SEM images of porous kaolin- and halloysite-based ceramics (shaped by freeze-tape casting and sintered at 1200 °C).</p>
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<p>SEM image of halloysite clay and peanut shell-based porous ceramic (shaped by uniaxial pressing and sintered at 1100 °C).</p>
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<p>SEM images of kaolinitic clay and peanut shell-based porous ceramics sintered at 900 (<b>a</b>) and 1100 °C (<b>b</b>) [<a href="#B56-ceramics-08-00003" class="html-bibr">56</a>].</p>
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<p>Example of arsenic (III) absorption mechanism onto a kaolinitic-based porous ceramic [<a href="#B56-ceramics-08-00003" class="html-bibr">56</a>].</p>
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19 pages, 2274 KiB  
Review
Urban Forestry in Sub-Saharan Africa: Challenges, Contributions, and Future Directions for Combating Climate Change and Restoring Forest Landscapes
by Sawaba Ale, Kossi Adjonou, Kossi Novigno Segla, Kossi Komi, Jean-Bosco Benewinde Zoungrana, Coffi Aholou and Kouami Kokou
Sustainability 2025, 17(1), 24; https://doi.org/10.3390/su17010024 - 24 Dec 2024
Viewed by 468
Abstract
Since the 1960s, the countries of sub-Saharan Africa have been affected by an urban crisis due to the demographic explosion in cities and the resulting land pressure. These issues have resulted in the proliferation of spontaneous housing areas, which call the future of [...] Read more.
Since the 1960s, the countries of sub-Saharan Africa have been affected by an urban crisis due to the demographic explosion in cities and the resulting land pressure. These issues have resulted in the proliferation of spontaneous housing areas, which call the future of urban vegetation and its degree of inclusion in city planning into question. To analyze the current scientific knowledge on the development of urban landscapes, particularly in sub-Saharan Africa, a literature review on urban forestry covering the period of 1998–2022 was carried out in order to better guide future research works towards a greater consideration of urban forestry and its contribution to combating the adverse effects of climate change. Out of the 110 scientific papers selected, 50 were considered relevant because of their direct link with urban forestry. Based on the analysis of these papers, a classification of the articles according to five themes was carried out. These themes are the management of green spaces in urban areas, the functions of trees in an urban environment, the development of urban forestry, the characterization of urban tree flora, and the financing of urban forestry. Thus, the “function of trees in an urban environment and the characterization of urban tree flora” are the most addressed issues, being raised in 42% and 28% of articles, respectively. “Financing urban forestry” is practically not addressed (2%). The analysis of the 50 selected articles reveals a significant geographic concentration of urban forestry research. Countries such as South Africa, Kenya, and Ghana are more represented in the literature, while vast regions in Central and Western Africa (e.g., the Democratic Republic of Congo, Chad, and Niger) are the object of minimal or no documented research with regard to urban forestry. Future research should address these underrepresented themes, as they are crucial for the long-term sustainability of urban forestry projects. Particularly, research on financing mechanisms is essential to unlocking sustainable funding sources for urban forestry, a critical barrier to its expansion. Full article
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<p>Map of Regions of Sub-Saharan Africa. Source: USDA, Economic Research Service.</p>
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<p>The synthesis of selection processes used to select the articles included in the analysis.</p>
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<p>Themes addressed in urban forestry research in sub-Saharan Africa.</p>
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<p>Topics covered in the literature by country. Legend: Management of green spaces in urban areas (GEVMU), Functions of trees in urban areas (FAMU), Development of urban forestry (DFU), Characterization of urban tree flora (CFAU) et Financing urban forestry (FFU).</p>
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<p>Articles published on urban forestry by country.</p>
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<p>Urban forestry articles by year.</p>
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18 pages, 4785 KiB  
Article
A Merging Approach for Improving the Quality of Gridded Precipitation Datasets over Burkina Faso
by Moussa Waongo, Juste Nabassebeguelogo Garba, Ulrich Jacques Diasso, Windmanagda Sawadogo, Wendyam Lazare Sawadogo and Tizane Daho
Climate 2024, 12(12), 226; https://doi.org/10.3390/cli12120226 - 20 Dec 2024
Viewed by 541
Abstract
Satellite precipitation estimates are crucial for managing climate-related risks such as droughts and floods. However, these datasets often contain systematic errors due to the observation methods used. The accuracy of these estimates can be enhanced by integrating spatial and temporal resolution data from [...] Read more.
Satellite precipitation estimates are crucial for managing climate-related risks such as droughts and floods. However, these datasets often contain systematic errors due to the observation methods used. The accuracy of these estimates can be enhanced by integrating spatial and temporal resolution data from in situ observations. Nevertheless, the accuracy of the merged dataset is influenced by the density and distribution of rain gauges, which can vary regionally. This paper presents an approach to improve satellite precipitation data (SPD) over Burkina Faso. Two bias correction methods, Empirical Quantile Mapping (EQM) and Time and Space-Variant (TSV), have been applied to the SPD to yield a bias-corrected dataset for the period 1991–2020. The most accurate bias-corrected dataset is then combined with in situ observations using the Regression Kriging (RK) method to produce a merged precipitation dataset. The findings show that both bias correction methods achieve similar reductions in RMS error, with higher correlation coefficients (approximately 0.8–0.9) and a normalized standard deviation closer to 1. However, EQM generally demonstrates more robust and consistent performance, particularly in terms of correlation and RMS error reduction. On a monthly scale, the superiority of EQM is most evident in June, September, and October. Following the merging process, the final dataset, which incorporates satellite information in addition to in situ observations, demonstrates higher performance. It shows improvements in the coefficient of determination by 83%, bias by 11.4%, mean error by 96.7%, and root-mean-square error by 95.5%. The operational implementation of this approach provides substantial support for decision-making in regions heavily reliant on rainfed agriculture and sensitive to climate variability. Delivering more precise and reliable precipitation datasets enables more informed decisions and significantly enhances policy-making processes in the agricultural and water resources sectors of Burkina Faso. Full article
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<p>Spatial distribution of annual precipitation with rain gauge network in the study area.</p>
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<p>Flow chart describing the steps of the satellite–rain gauge data merging approach.</p>
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<p>Schematic of the quantile mapping method, with a blue arrow highlighting the quantile-based systematic bias. Adapted from [<a href="#B46-climate-12-00226" class="html-bibr">46</a>].</p>
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<p>Spatial distribution of rain gauge stations across Burkina Faso (<b>a</b>) and percentage of data completeness at quality-controlled stations for the period 1991–2020 (<b>b</b>). The color scale indicates the percentage of available daily precipitation records at each station.</p>
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<p>Comparison of average daily precipitation between TAMSAT corrected by TSV (<b>b</b>) and EQM (<b>c</b>) with reference from in situ observations (<b>a</b>).</p>
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<p>Taylor diagram illustrating the statistics of inter-comparison between three datasets: uncorrected, TSV corrected and EQM corrected for the period of 1991–2020.</p>
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<p>Taylor diagram illustrating the statistics of inter-comparison between three precipitation datasets at a monthly scale for the wet season.</p>
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<p>Comparison of extreme daily precipitation between TAMSAT corrected by TSV (<b>b</b>) and EQM (<b>c</b>) with reference from in situ observations (<b>a</b>).</p>
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<p>Average daily precipitation from TAMSAT satellite data corrected using (<b>a</b>) EQM method and (<b>b</b>) merging the bias-corrected datasets with rain gauge data using the merging approach, both compared with ground-based observations.</p>
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<p>The spatial distribution of the merging for rainy events on 7 May 2010 (<b>top</b>), 29 July 2010 (<b>middle</b>), and 6 October 2010 (<b>bottom</b>). These three periods correspond to the early season, mid-season, and the end season of the wet season, respectively. Dataset sources are station observations (<b>a</b>,<b>d</b>,<b>g</b>), original TAMSAT data (<b>b</b>,<b>e</b>,<b>h</b>), and merged products (<b>c</b>,<b>f</b>,<b>i</b>).</p>
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13 pages, 989 KiB  
Article
Simulation and Optimization of Available Local Feed Resources for Dairy Cattle in Burkina Faso
by Rayinwendé Irène Sawadogo, Vinsoun Millogo, Mariétou Sissao, Michel Kere, Wendpayanguedé Alain Sawadogo and Modou Séré
Appl. Sci. 2024, 14(24), 11891; https://doi.org/10.3390/app142411891 - 19 Dec 2024
Viewed by 351
Abstract
The poor quality of natural pastures in the dry season does not make it possible to meet dairy cows’ requirements for milk production in Burkina Faso and in most West African countries. Therefore, it is urgent to find an alternative by developing a [...] Read more.
The poor quality of natural pastures in the dry season does not make it possible to meet dairy cows’ requirements for milk production in Burkina Faso and in most West African countries. Therefore, it is urgent to find an alternative by developing a full diet from locally available ingredients. The objective was to determine a diet for dairy cattle based on locally available ingredients in the peri-urban area of Ouagadougou. A progressive methodology was used. Thus, a survey was conducted ontoonton 30 dairy farms. This survey was followed by chemical analysis, for which the most dominant forage and concentrate ingredients were selected. Secondly, the recording of milk and on-farm ingredient use was also carried out using Op-Ration software (Op-Ration version V3.4.5.0) in order to compare and determine the most suitable diets. The data from the survey were subjected to descriptive statistics using SPSS version 20. Those from chemical analysis, milk recording, and ingredient assessment on the farm were subjected to a statistical method using the software Minitab version.18.1.0.0 setup. The results showed two dominant forage species, Sorghum (84.85%) and Pennisetum pedicellatum (90.91%), and two dominant concentrates, corn bran (32%) and cottonseed meal (26%), used by dairy farmers. From these ingredients and simulating the requirements of 400 kgPV0.75 of lactating cows, a diet assessment was carried out at early, middle, and end lactation. The results showed that at the beginning of lactation, the diet consisted of 6.73 kg of forage and 6.59 kg of concentrate for 13.5 L as the main objective of milk production. The diet for mid-lactation was 8 kg of forage and 6.5 kg of concentrate for 15.5 L per day and 5.7 kg of forage and 3.8 kg of concentrate for the end of lactation. The results of this study show that it is possible to manufacture a complete ration for dairy cows at different stages of lactation from locally available forages and concentrates in the peri-urban area of Ouagadougou. This type of method could be applied to other regions from local forages and concentrates for milk production. Full article
(This article belongs to the Special Issue Environmental Management in Milk Production and Processing)
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<p>Map of the study area (BNTD).</p>
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<p>Lactation curves of milk yield, fat, protein, and lactose contents.</p>
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13 pages, 1930 KiB  
Article
Pan African Vivax and Ovale Network (PAVON) Malaria Diagnostic Competency Training: Offering Training Opportunities to Impact Malaria Elimination Strategies in Sub-Saharan Africa
by Amidou Diarra, John Ayivase, Dolen G. Mompati, Issiaka Soulama, Mamoudou Cissé, Nancy O. Duah-Quashie, Ben Gyan, Anthony Z. Dongdem, Wisdom K. Takramah, Grace K. Ababio, Claude Oeuvray, James Mulry, Beatrice Greco, Jutta Reinhard-Rupp and Isaac K. Quaye
Trop. Med. Infect. Dis. 2024, 9(12), 308; https://doi.org/10.3390/tropicalmed9120308 - 19 Dec 2024
Viewed by 562
Abstract
PAVON has developed a malaria microscopy competency training scheme to augment competency in malaria microscopy. Here, data accrued from training activities between 2020 and 2023 in Botswana are presented. Three trainings were done for 37 central and peripheral level technicians for a two-week [...] Read more.
PAVON has developed a malaria microscopy competency training scheme to augment competency in malaria microscopy. Here, data accrued from training activities between 2020 and 2023 in Botswana are presented. Three trainings were done for 37 central and peripheral level technicians for a two-week period. The scheme consisted of basic theory on Plasmodium parasites, malaria epidemiology and diagnosis. The practicals focused on standard slide preparation, staining, parasite detection, speciation and counting. Scores were assessed by the Wilcoxon signed rank test. Participants who excelled joined the WHO External Competency Assessment for Malaria Microscopy (ECAMM). The median competency scores for the three trainings were detection: 100 (IQR = 94–100), 100 (IQR = 94–100) and 92 (IQR = 92–100), respectively, from pre-test scores of 40 (IQR = 27–54), 44 (IQR = 32–52) and 20 (IQR = 10–40) (z = 2.937, p < 0.003, z = 3.110, p = 0.002 and (z = 2.251, p = 0.024), respectively. Speciation: 93 (IQR = 86–96), 81 (IQR = 73–96) and 88, (IQR = 88–100) from pre-test scores of 50 (IQR = 30–50), 36 (IQR = 20–45) and 17 (IQR = 17–50) (z = 2.936, p < 0.003, z = 3.152, p = 0.002 and z = 3.237, p = 0.001). The competency scores achieved in the ECAMM were 98/98 and 97/98 for sensitivity and specificity pre- and post-tests detection. The training was effective in raising the competency skills of participants and is open to NMPs (National Malaria Programs) for critical capacity building. Full article
(This article belongs to the Special Issue The Global Burden of Malaria and Control Strategies)
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<p>Scheme for basic guidelines on slide preparation and scores for competency.</p>
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<p>Criteria for defining the quality of the blood smears.</p>
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<p>Comparing writing theory test scores before and after training. The median values are highlighted in yellow bounded by the upper and lower quartiles. A filled dot is an outlier.</p>
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<p>Comparing identification test scores before and after the trainings. Two post-tests were conducted in the November 2022 and November 2023 trainings. The median values are highlighted in yellow bounded by the upper and lower quartiles. Filled dots are outliers.</p>
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<p>Comparing the speciation test scores before and after the trainings. Two post-tests were conducted in the November 2022 and November 2023 trainings. The median values are highlighted in yellow bounded by the upper and lower quartiles. Filled dots are outliers.</p>
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<p>Comparing counting test scores before and after the trainings. Two post-tests were conducted in the November 2022 and November 2023 trainings. The median values are highlighted in yellow bounded by the upper and lower quartiles. Filled dots are outliers.</p>
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<p>Comparing mixed infection detection test scores before and after the trainings. The median values are highlighted in yellow bounded by the upper and lower quartiles.</p>
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15 pages, 3359 KiB  
Article
Optimization and Standardization of the Extraction Method of Balanites aegyptiaca Del. Seeds (Zygophyllaceae) Used in the Formulation of an Antiparasitic Phytomedicine
by Mohamed Bonewendé Belemlilga, Salfo Ouedraogo, Gilchrist Abdoul Laurent Boly, Do Harouna Dao, Jonas Tiami Coulibaly, Jean Claude Romaric Pingdwindé Ouedraogo, Souleymane Compaoré, Sidiki Traore, Moumouni Koala, Estelle Noëla Hoho Youl, Lazare Belemnaba, Félix Bondo Kini, Aristide Traore, Séni Kouanda and Sylvin Ouedraogo
Pharmaceuticals 2024, 17(12), 1698; https://doi.org/10.3390/ph17121698 - 17 Dec 2024
Viewed by 341
Abstract
Background/Objectives: Balanites aegyptiaca Del. (Zygophyllaceae) is widely used in traditional medicine, both human and veterinary, throughout Africa for its many properties, including antiparasitic properties. This experimental study aims to optimize the extraction conditions of the seeds of Balanites aegyptiaca Del. Methods: Aqueous maceration [...] Read more.
Background/Objectives: Balanites aegyptiaca Del. (Zygophyllaceae) is widely used in traditional medicine, both human and veterinary, throughout Africa for its many properties, including antiparasitic properties. This experimental study aims to optimize the extraction conditions of the seeds of Balanites aegyptiaca Del. Methods: Aqueous maceration was carried out with mass-to-volume ratios of 40%, 30%, 20%, 10% and 5% and extraction times of 6, 12, 24, 36 and 48 h. Extraction yields, phytochemical screening, saponins assay, antioxidant activities ABTS+ free radical scavenging activities, Ferric-reducing antioxidant power (FRAP) assay and antiparasitic tests on Heligmosomoides bakeri were used to compare the different extracts. Results: The pharmaco-chemical study generally showed that aqueous maceration gave the best results, with a mass/volume ratio of 10% after 12 h of maceration. The yield obtained was 28.03% with a saponins content of 13.81 mg/g. The antioxidant activities were 4.25 ± 0.17 µg/mL by the ABTS method and 0.739 µg/mL by the FRAP method. The larvicidal activity also showed that the 10% 12 h extract produced 100% larval mortality from 25 µg/mL. Conclusions: These data provide a basis for guiding the extraction process parameters in producing this antiparasitic phytomedicine. Full article
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<p>Almond powder (<b>a</b>) and lyophilized extract (<b>b</b>) of <span class="html-italic">Balanites aegyptiaca</span> seeds.</p>
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<p>The residual moisture content of different lyophilizate. ** <span class="html-italic">p</span> &lt; 0.01, **** <span class="html-italic">p</span> &lt; 0.0001 is considered significant compared with the other % macerates (two-way ANOVA followed by the “Tukey” multiple comparison test; ns is considered not significant). <span class="html-italic">n</span> = 3.</p>
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<p>Extraction yield histogram of aqueous macerates mass/volume ratio as a function of time (hours). *** <span class="html-italic">p</span> &lt; 0.01, **** <span class="html-italic">p</span> &lt; 0.0001 is considered significant compared with the other % macerates (two-way ANOVA followed by the “Tukey” multiple comparison test; ns is considered not significant). <span class="html-italic">n</span> = 3.</p>
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<p>Analytical TLC profile of different extracts. (<b>a</b>) The presence of saponins revealed by sulfuric anisaldehyde after heating the plate observed in visible light. (<b>b</b>) Presence of flavonoids (at 254 nm) detected by NEU reagent.</p>
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<p>Larvicidal effect of <span class="html-italic">B. aegyptiaca</span> extracts and the standard on <span class="html-italic">H. bakeri</span>.</p>
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<p>Whole plant (<b>a</b>), fruits (<b>b</b>) and isolated saponins (<b>c</b>,<b>d</b>) of <span class="html-italic">Balanites aegyptiaca</span>.</p>
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15 pages, 1885 KiB  
Article
From Intact to Highly Degraded Mitochondrial Genes in Trypanosoma vivax: New Insights into Introduction from Africa and Adaptation to Exclusive Mechanical Transmission in South America
by Herakles A. Garcia, Adriana C. Rodrigues, Carla M. F. Rodrigues, Andrea S. Florentin, Angélica M. Bethencourt, Armando Reyna-Bello, María Augusta Chávez-Larrea, Carlos L. Pereira, Zacharia Bengaly, Desie Sheferaw, Agapitus B. Kato, Fernando Paiva, Erney F. P. Camargo and Marta M. G. Teixeira
Parasitologia 2024, 4(4), 390-404; https://doi.org/10.3390/parasitologia4040035 - 13 Dec 2024
Viewed by 706
Abstract
Trypanosoma vivax displays distinct mitochondrial genomes: intact in Africa and degraded in South America. This finding, formerly reported from four isolates, suggested an association between mitochondrial mutations and adaptation to exclusive mechanical transmission outside Africa, circumventing mitochondrial activity essential for cyclical transmission by [...] Read more.
Trypanosoma vivax displays distinct mitochondrial genomes: intact in Africa and degraded in South America. This finding, formerly reported from four isolates, suggested an association between mitochondrial mutations and adaptation to exclusive mechanical transmission outside Africa, circumventing mitochondrial activity essential for cyclical transmission by tsetse flies. Here, we characterized the mitochondrial ND7 and COIII genes in 25 South American isolates of T. vivax from livestock from Argentina, Brazil, Colombia, Ecuador, and Venezuela, as well as in 11 African isolates from Mozambique, Ethiopia, Uganda, Nigeria, and Burkina Faso from tsetse flies, antelopes, and cattle. Phylogenetic inference supported the lineages TVV (Trypanosoma vivax vivax) and TVL (Trypanosoma vivax-like), predominant in South America/West Africa and East Africa, respectively. Both ND7 and COIII genes were intact in all African TVV and TVL isolates and, for the first time, in 10 South American isolates from Brazil. Remaining South American isolates exhibited some degree of gene erosion, including ~142 bp deletion at the 3′ end of ND7 disclosed in Venezuela and Colombia, ~163 bp deletion at the 3′ end of ND7 in Brazil and Argentina, and ~750 bp deletion from the 5′ end of ND7 to the 3′ end of COIII in Venezuela. Comprehensive analyses of whole mitochondrial genomes from additional South American and African isolates are crucial for formulating reliable hypotheses regarding the origin, absence, and rapid mitochondrial degradation observed in South American T. vivax. Full article
(This article belongs to the Special Issue The Molecular Epidemiology of Parasites)
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<p>Geographical origin of <span class="html-italic">Trypanosoma vivax</span> isolates from tsetse cyclical transmission in African countries and mechanical transmission in South American endemic and outbreak areas. These isolates were obtained from wild animals and livestock with infections ranging from severely sick to asymptomatic and tsetse flies. Sampling areas in South America include Venezuela (states of Tru, Trujillo; Apu, Apure; Anz, Anzoátegui; Mon, Monagas), Brazil (states of PA, Pará; PB, Paraíba; MS, Mato Grosso do Sul; SP, São Paulo), and other countries (see <a href="#parasitologia-04-00035-t001" class="html-table">Table 1</a> for sampling details).</p>
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<p>Schematic representation of polymorphisms, including mutations and deletions, on the contiguous NADH dehydrogenase subunit 7 (<span class="html-italic">ND7</span>) and cytochrome oxidase subunit III (<span class="html-italic">COIII</span>) genes from <span class="html-italic">Trypanosoma vivax</span> isolates from South America and Africa. TviMit-0 to TviMit-3 correspond to the four mitochondrial profiles determined for the isolates characterized in this and in a previous study [<a href="#B37-parasitologia-04-00035" class="html-bibr">37</a>,<a href="#B38-parasitologia-04-00035" class="html-bibr">38</a>]. The vertical bars represent single nucleotide polymorphisms. Symbols indicating the origin of the isolates are shown in <a href="#parasitologia-04-00035-f001" class="html-fig">Figure 1</a>.</p>
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<p>Phylogenetic relationships of <span class="html-italic">Trypanosoma vivax</span> isolates from Africa and South America based on NADH dehydrogenase subunit 7 (<span class="html-italic">ND7</span>) and cytochrome oxidase subunit III (<span class="html-italic">COIII</span>) sequences. The branching pattern supports distant lineages TVV and TVL, predominantly comprising isolates from South America/West Africa and East Africa, respectively. Within TVV, the isolates cluster according to TviMit-0, TviMit-1, and TviMit-2 mitochondrial profiles. The analysis was performed using the Neighbour-Net method with the K2 parameter, and nodal support was estimated with 500 bootstrap replicates. SA, South America; WA, West Africa (Nigeria); EA, East Africa (Eth, Ethiopia, Ug, Uganda, Mz, Mozambique). Brasilian States: PB, Paraíba; PA, Pará; SP, São Paulo; MS, Mato Grosso do Sul.</p>
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2 pages, 199 KiB  
Comment
Observations and Reflections from Sub-Saharan Africa. Comment on Shahait, M. Urology Practice in Low- to Middle-Income Countries. Soc. Int. Urol. J. 2024, 5, 374–375
by Saleh A. Nedjim, Kaleab H. Gebreselassie, Mahamat A. Mahamat, Ouima J. D. Ziba, Anteneh T. Kifle, Abdullahi Khalid, Rimtebaye Kimassoum, Choua Ouchemi and Alain Khassim Ndoye
Soc. Int. Urol. J. 2024, 5(6), 867-868; https://doi.org/10.3390/siuj5060067 - 12 Dec 2024
Viewed by 238
Abstract
Shahait’s article on “Urology Practice in Low- to Middle-Income Countries” [...] Full article
25 pages, 7197 KiB  
Article
Malaria Prevention for Pregnant Women and Under-Five Children in 10 Sub-Saharan Africa Countries: Socioeconomic and Temporal Inequality Analysis
by Denis Okova, Akim Tafadzwa Lukwa, Robinson Oyando, Paidamoyo Bodzo, Plaxcedes Chiwire and Olufunke A. Alaba
Int. J. Environ. Res. Public Health 2024, 21(12), 1656; https://doi.org/10.3390/ijerph21121656 - 11 Dec 2024
Viewed by 843
Abstract
Background: Malaria remains a public health challenge in low- and middle-income countries (LMICs). Despite gains from strategies like Insecticide-Treated Nets (ITNs) and Intermittent Preventive Treatment during pregnancy (IPTp), significant socioeconomic inequalities persist, particularly among pregnant women and children under five. This study analyzed [...] Read more.
Background: Malaria remains a public health challenge in low- and middle-income countries (LMICs). Despite gains from strategies like Insecticide-Treated Nets (ITNs) and Intermittent Preventive Treatment during pregnancy (IPTp), significant socioeconomic inequalities persist, particularly among pregnant women and children under five. This study analyzed temporal and socioeconomic inequalities in malaria prevention in sub-Saharan Africa (SSA). Methods: Nationally representative Demographic Health Surveys from 10 SSA countries (Mozambique, Burkina Faso, Tanzania, Côte d’Ivoire, Madagascar Kenya, Rwanda, Nigeria, Uganda, and Cameroon) were used, comparing two time periods. Changes in ITN use by pregnant women and children under five, as well as IPTp coverage, were assessed. Inequalities based on socioeconomic status (SES) and residence were analyzed using the Erreygers Normalized Concentration Index and Theil index. Results: The results revealed significant variability in ITN use and IPTp coverage within countries. Eight countries showed improvements in ITN use during pregnancy, with Nigeria seeing a 173.9% increase over five years. Burkina Faso and Tanzania consistently reported high ITN use (~87%) in children under five. IPTp coverage increased in all countries except Kenya. Decomposition using the Theil index indicated that within-group inequalities, particularly based on SES and residence, were the primary drivers of disparities. Conclusions: To ensure progress toward universal health coverage, malaria prevention programs must prioritize vulnerable populations and be continuously evaluated. Full article
(This article belongs to the Special Issue Socio-Economic Inequalities in Child Health)
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<p>Map of Sub-Saharan Africa Highlighting the 10 Countries Included in this Study.</p>
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<p>Erregyers Normalised Concentration Curves for ITN use and IPTp coverage in 10 countries in SSA, various years.</p>
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<p>Erregyers Normalised Concentration Curves for ITN use and IPTp coverage in 10 countries in SSA, various years.</p>
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<p>Erregyers Normalised Concentration Curves for ITN use and IPTp coverage in 10 countries in SSA, various years.</p>
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<p>Erregyers Normalised Concentration Curves for ITN use and IPTp coverage in 10 countries in SSA, various years.</p>
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15 pages, 524 KiB  
Study Protocol
Describing Biological Vulnerability in Small, Vulnerable Newborns in Urban Burkina Faso (DenBalo): Gut Microbiota, Immune System, and Breastmilk Assembly
by Lionel Olivier Ouédraogo, Lishi Deng, Cheick Ahmed Ouattara, Anderson Compaoré, Moctar Ouédraogo, Alemayehu Argaw, Carl Lachat, Eric R. Houpt, Queen Saidi, Filomeen Haerynck, Justin Sonnenburg, Meghan B. Azad, Simon J. Tavernier, Yuri Bastos-Moreira, Laeticia Celine Toe and Trenton Dailey-Chwalibóg
Nutrients 2024, 16(23), 4242; https://doi.org/10.3390/nu16234242 - 9 Dec 2024
Viewed by 722
Abstract
Background: Small vulnerable newborns (SVNs), including those born preterm, small for gestational age, or with low birth weight, are at higher risk of neonatal mortality and long-term health complications. Early exposure to maternal vaginal microbiota and breastfeeding plays a critical role in [...] Read more.
Background: Small vulnerable newborns (SVNs), including those born preterm, small for gestational age, or with low birth weight, are at higher risk of neonatal mortality and long-term health complications. Early exposure to maternal vaginal microbiota and breastfeeding plays a critical role in the development of the neonatal microbiota and immune system, especially in low-resource settings like Burkina Faso, where neonatal mortality rates remain high. Objectives: The DenBalo study aims to investigate the role of maternal and neonatal factors, such as vaginal and gut microbiota, immune development, and early nutrition, in shaping health outcomes in SVNs and healthy infants. Methods: This prospective cohort observational study will recruit 141 mother-infant pairs (70 SVNs and 71 healthy controls) from four health centers in Bobo-Dioulasso, Burkina Faso. The mother-infant pairs will be followed for six months with anthropometric measurements and biospecimen collections, including blood, breast milk, saliva, stool, vaginal swabs, and placental biopsies. Multi-omics approaches, encompassing metagenomics, metabolomics, proteomics, and immune profiling, will be used to assess vaginal and gut microbiota composition and functionality, immune cell maturation, and cytokine levels at critical developmental stages. Conclusions: This study will generate comprehensive data on how microbiota, metabolomic, and proteomic profiles, along with immune system development, differ between SVNs and healthy infants. These findings will guide targeted interventions to improve neonatal health outcomes and reduce mortality, particularly in vulnerable populations. Full article
(This article belongs to the Section Pediatric Nutrition)
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<p>Flow chart of the DenBalo study schedule.</p>
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18 pages, 5713 KiB  
Article
Comparative Trend Analysis of Precipitation Indices in Several Towns of the Sirba River Catchment (Burkina Faso) from CHIRPS and TAMSAT Rainfall Estimates
by Giorgio Cannella, Alessandro Pezzoli and Maurizio Tiepolo
Climate 2024, 12(12), 208; https://doi.org/10.3390/cli12120208 - 4 Dec 2024
Viewed by 747
Abstract
The increasingly frequent pluvial flood of West African urban settlements indicates the need to investigate the drivers of local rainfall changes. However, meteorological stations are few, unevenly distributed, and work irregularly. Daily satellite rainfall datasets can be used. Nevertheless, these products often need [...] Read more.
The increasingly frequent pluvial flood of West African urban settlements indicates the need to investigate the drivers of local rainfall changes. However, meteorological stations are few, unevenly distributed, and work irregularly. Daily satellite rainfall datasets can be used. Nevertheless, these products often need to be more accurate due to sensor errors and limitations in retrieval algorithms. The problem is, therefore, how to characterize rainfall where there is a need for ground-based rainfall records or incomplete series. This study aims to characterize urban rainfall using two satellite datasets. The analysis was carried out in the Sirba river catchment, Burkina Faso, using the Climate Hazards Group InfraRed Precipitation with Station data (CHIRPS) and the Tropical Applications of Meteorology using SATellite and ground-based data (TAMSAT) datasets. Ten indices from the Expert Team on Climate Change Detection and Indices (ETCCDI) of precipitation were calculated, and their statistical trends were evaluated from 1983 to 2023. The study introduces two key innovations: a comparative analysis of precipitation trends using two satellite datasets and applying this analysis to towns within a previously understudied 39,138 km2 catchment area that is frequently flooded. Both datasets agree on the increase of (i) annual cumulative rainfall over all towns, (ii) five-day maximum rainfall over the town of Manni, (iii) rainfall due to very wet days in Gayéri, (iv) days of heavy rainfall in Bogandé, Manni and Yalgho, and (v) days of very heavy rainfall in Yalgho. These findings suggest the need for targeted pluvial flood prevention measures in towns with increasing trends in heavy rainfall. Full article
(This article belongs to the Special Issue Advances of Flood Risk Assessment and Management)
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<p>The Sirba River catchment.</p>
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<p>Flowchart of the analysis.</p>
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<p>Trends for indices RX5 (<b>a</b>,<b>b</b>), R95P (<b>c</b>,<b>d</b>), R10mm (<b>e</b>,<b>f</b>), and R20mm (<b>g</b>,<b>h</b>) from CHIRPS and TAMSAT datasets. The size of the triangles is proportional to the magnitude of the trends. Red triangles identify trends with statistical significance. (1-Arbinda; 2-Bogandé; 3-Boulsa; 4-Gayéri; 5-Manni; 6-Pissila; 7-Sebba; 8-Yalgho).</p>
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<p>Trends for indices RX5 (<b>a</b>,<b>b</b>), R95P (<b>c</b>,<b>d</b>), R10mm (<b>e</b>,<b>f</b>), and R20mm (<b>g</b>,<b>h</b>) from CHIRPS and TAMSAT datasets. The size of the triangles is proportional to the magnitude of the trends. Red triangles identify trends with statistical significance. (1-Arbinda; 2-Bogandé; 3-Boulsa; 4-Gayéri; 5-Manni; 6-Pissila; 7-Sebba; 8-Yalgho).</p>
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<p>The trend for the CDD (<b>a</b>,<b>b</b>), CWD (<b>c</b>,<b>d</b>), and SDII (<b>e</b>,<b>f</b>) indices from CHIRPS and TAMSAT datasets. The orientation of the triangles is related to the direction of the trend (upward for positive trends, downward for negative trends). The size of the triangles is proportional to the magnitude of the trends. Red triangles identify trends with statistical significance. (1-Arbinda; 2-Bogandé; 3-Boulsa; 4-Gayéri; 5-Manni; 6-Pissila; 7-Sebba; 8-Yalgho).</p>
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<p>Flowchart and infographic of the comparison of rainfall trends at Bogandé. In blue are the elements of agreement between station and SPPs datasets.</p>
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26 pages, 1954 KiB  
Systematic Review
Biological Hazards and Indicators Found in Products of Animal Origin in Cambodia from 2000 to 2022: A Systematic Review
by Shwe Phue San, Rortana Chea, Delia Grace, Kristina Roesel, Sothyra Tum, Stephen Young, Tumnoon Charaslertrangsi, Nazanin Zand, Shetty Seetharama Thombathu, Ra Thorng, Leab Kong, Kuok Fidero and Linda Nicolaides
Int. J. Environ. Res. Public Health 2024, 21(12), 1621; https://doi.org/10.3390/ijerph21121621 - 3 Dec 2024
Viewed by 906
Abstract
Biological hazards in products of animal origin pose a significant threat to human health. In Cambodia, there are few comprehensive data and information on the causes of foodborne diseases or risks. To date, there has been no known published study similar to this [...] Read more.
Biological hazards in products of animal origin pose a significant threat to human health. In Cambodia, there are few comprehensive data and information on the causes of foodborne diseases or risks. To date, there has been no known published study similar to this review. This systematic review is aimed to investigate the prevalence of biological hazards and their indicators in products of animal origin from 2000 to 2022. The main objective of this study was also to contribute to strengthening Cambodia’s food control system. This review followed the established “Preferred Reporting Items for Systematic Reviews and Meta-Analyses” (PRISMA) guidelines. In total, 46 studies were retained for complete review. Most studies (n = 40) had been conducted by or with external researchers, reflecting the under-resourcing of the National Food Control System in terms of surveillance; areas outside the capital were relatively understudied, reflecting evidence found in Ethiopia and Burkina Faso. Five categories of hazards were reported with the highest number of studies on fish parasites. Marketed fish, often originating from different countries, had a higher mean value of parasite prevalence (58.85%) than wild-caught fish (16.46%). Viral pathogens in bat meat presented a potential spillover risk. Many potentially important hazards had not yet been studied or reported (e.g., Norovirus, Shigella, toxin-producing Escherichia coli, and Vibrio cholerae). The findings of our review highlighted significant urgencies for national competent authorities to enhance food hygiene practices along the production chain, tackle import control, and enforce the implementation of a traceability system, alongside more research collaboration with neighboring countries and key trading partners. It is crucial to conduct more extensive research on food safety risk analysis, focusing on the identification and understanding of various biological hazards and their associated risk factors in food. Full article
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<p>PRISMA flowchart showing identification, screening, and inclusion of eligible articles reporting foodborne biological hazards in animals and POAOs in Cambodia from 2000 to 2022.</p>
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<p>Evidence of frequency of research for food biological hazards in animals and POAOs conducted by national initiatives, joint initiatives, and international institutions in Cambodia (2000 to 2022).</p>
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<p>Evidence of frequency of studies identified for different types of biological hazards in animals and POAOs in Cambodia from 2000 to 2022.</p>
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<p>Number of publications of biological hazards in animals and POAOs in Cambodia for different intervals from 2000 to 2022.</p>
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<p>Number of studies included in the systematic review in each province in Cambodia from 2000 to 2022 (the map of Cambodia with provinces was downloaded from <a href="http://Vemaps.com" target="_blank">Vemaps.com</a> (accessed on 17 December 2023).</p>
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<p>Evidence of the prevalence of different types of parasites in fish and fishery products.</p>
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<p>Evidence of parasite prevalence in fish and fishery products taken from three different points of sampling showing the significant difference between “nature” (a) and “market” (b).</p>
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16 pages, 11615 KiB  
Article
Assessing the Impacts of Climate Change on Rainfed Maize Production in Burkina Faso, West Africa
by Moussa Waongo, Patrick Laux, Amadou Coulibaly, Souleymane Sy and Harald Kunstmann
Atmosphere 2024, 15(12), 1438; https://doi.org/10.3390/atmos15121438 - 29 Nov 2024
Viewed by 611
Abstract
Smallholder rainfed agriculture in West Africa is vital for regional food security and livelihoods, yet it remains highly vulnerable to climate change. Persistently low crop yields, driven by high rainfall variability and frequent climate hazards, highlight the urgent need for evidence-based adaptation strategies. [...] Read more.
Smallholder rainfed agriculture in West Africa is vital for regional food security and livelihoods, yet it remains highly vulnerable to climate change. Persistently low crop yields, driven by high rainfall variability and frequent climate hazards, highlight the urgent need for evidence-based adaptation strategies. This study assesses the impact of climate change on maize yields in Burkina Faso (BF) using a calibrated AquaCrop model and recent climate projections. AquaCrop was calibrated using district-level maize yields from 2009 to 2022 and a genetic optimization technique. Climate change impacts were then simulated using two socioeconomic scenarios (SSP2–4.5 and SSP5–8.5) for the periods 2016–2045 and 2046–2075. Climate projections show that Burkina Faso will experience temperature increases of 0.5–3 °C and decreased precipitation, with the most severe rainfall reductions in the country’s southern half, including the crucial southwestern agricultural zone. Maize yields will predominantly decrease across the country, with projected losses reaching 20% in most regions. The southwestern agricultural zone, critical for national food production, faces substantial yield decreases of up to 40% under the SSP5-8.5 scenario. In light of these findings, future research should employ the calibrated AquaCrop model to evaluate specific combinations of adaptation strategies. These strategies include optimized planting windows, field-level water management practices, and optimal fertilizer application schedules, providing actionable guidance for smallholder farmers in West Africa. Full article
(This article belongs to the Special Issue Climate Change and Agriculture: Impacts and Adaptation)
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<p>Annual rainfall distribution (average of 1981–2010) in the study area is depicted in section (<b>a</b>), and the Land Use, Land Cover (LULC) for the year 2013 in Burkina Faso is shown in section (<b>b</b>). The agricultural zone is highlighted in yellow, with the highest density located in the southwest of Burkina Faso. Rainfall data from the rain gages network have been provided by the National Meteorological Agency in Burkina Faso, while the LULC data were obtained from CILSS, 2016 [<a href="#B21-atmosphere-15-01438" class="html-bibr">21</a>]. Quantum GIS (QGIS) version 3.22, available at <a href="https://www.qgis.org/" target="_blank">https://www.qgis.org/</a> (accessed on 10 March 2024), was used for the creation of the study area and LULC map.</p>
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<p>Spatial distribution of soil texture (<b>a</b>) for the first layer and maize average yield (<b>b</b>) in BF.</p>
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<p>Flowchart of maize yield simulation using SSP2–4.5 and SSP5–8.5 data in combination with AquaCrop model.</p>
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<p>Performance metrics R<sup>2</sup> (<b>a</b>) and RAE (<b>b</b>) in the study area. Pairwise observed and simulated maize yields for the period 2009–2022 have been used to compute the R<sup>2</sup> and RAE for each grid cell across BF.</p>
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<p>Ensemble mean (<b>left</b>) and standard deviation (<b>right</b>) of precipitation. Statistics are calculated based on projected annual precipitation changes from 15 GCMs.</p>
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<p>Ensemble mean (<b>left</b>) and standard deviation (<b>right</b>) of temperature. Statistics are calculated based on projected annual mean temperature changes from 15 GCMs.</p>
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<p>Projected maize yield changes for emission scenarios SSP2–4.5 (<b>top</b>) and SSP5–8.5 (<b>bottom</b>). For each grid cell, the yield deviation represents the ensemble mean from the 15 GCMs.</p>
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<p>Maize yield deviation [%] through GCMs in BF. For each GCM, all grid cells within BF are considered to compute the statistics.</p>
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