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Keywords = Animal-Assisted Interventions

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14 pages, 259 KiB  
Article
Behavioural Profile Differences Between Cats in Animal-Assisted Services (AAS) and Non-AAS Cats Using the Fe-BARQ in Flanders
by Joni Delanoeije, Christel Palmyre Henri Moons, Els Helena Karel Anna Peeters and Patricia Pendry
Animals 2025, 15(1), 33; https://doi.org/10.3390/ani15010033 - 26 Dec 2024
Viewed by 186
Abstract
The engagement of cats in animal-assisted services (AAS) is increasing. This is surprising given that feline behavioural needs have been perceived as contradictory to conditions associated with AAS engagement, leading to the assumption that cats as a species are not suitable for AAS. [...] Read more.
The engagement of cats in animal-assisted services (AAS) is increasing. This is surprising given that feline behavioural needs have been perceived as contradictory to conditions associated with AAS engagement, leading to the assumption that cats as a species are not suitable for AAS. However, important within-species variability in behavioural traits nuancing this assumption has not been addressed. Therefore, this study aimed to map the behavioural profiles of cats engaged in AAS and non-AAS cats to detect behavioural profile differences between these groups. Such differences could point to AAS cats being selected to engage in AAS based upon particular behavioural traits or, relatedly, to AAS cats coping differently with AAS features. Using a Flemish translation of the Feline Behavioural Assessment and Research Questionnaire (Fe-BARQ), cross-sectional survey data on various cats’ (N = 474) behavioural indices were collected. Using Mann–Whitney U-tests, results indicate that AAS cats significantly differed from non-AAS cats on five out of 19 behavioural subscales. Specifically, AAS cats scored higher on sociability with people, attention seeking, sociability with cats and predatory behaviour, and they scored lower on resistance to restraint. While this study did not allow for assessing explanations for these differences, findings suggest that cats in AAS may be intentionally or unintentionally selected for behavioural traits perceived as desirable for engagement in AAS. While we refrain from drawing conclusions regarding the “suitability” of cats based solely on our findings, our study contributes to the ongoing discourse surrounding critical themes around AAS-engaging cats. Furthermore, our findings provide a first indication of the reliability of a translation of a well-validated measurement tool suitable to map behavioural traits of cats in Flanders engaged in AAS. Full article
(This article belongs to the Section Human-Animal Interactions, Animal Behaviour and Emotion)
21 pages, 970 KiB  
Systematic Review
Telerehabilitation and Its Impact Following Stroke: An Umbrella Review of Systematic Reviews
by Bayan Alwadai, Hatem Lazem, Hajar Almoajil, Abigail J. Hall, Maedeh Mansoubi and Helen Dawes
J. Clin. Med. 2025, 14(1), 50; https://doi.org/10.3390/jcm14010050 - 26 Dec 2024
Viewed by 153
Abstract
Objectives: To summarize the impact of various telerehabilitation interventions on motor function, balance, gait, activities of daily living (ADLs), and quality of life (QoL) among patients with stroke and to determine the existing telerehabilitation interventions for delivering physiotherapy sessions in clinical practice. [...] Read more.
Objectives: To summarize the impact of various telerehabilitation interventions on motor function, balance, gait, activities of daily living (ADLs), and quality of life (QoL) among patients with stroke and to determine the existing telerehabilitation interventions for delivering physiotherapy sessions in clinical practice. Methods: Six electronic databases were searched to identify relevant quantitative systematic reviews (SRs). Due to substantial heterogeneity, the data were analysed narratively. Results: A total of 28 systematic reviews (n = 245 primary studies) were included that examined various telerehabilitation interventions after stroke. Motor function was the most studied outcome domain across the reviews (20 SRs), followed by ADL (18 SRs), and balance (14 SRs) domains. For primary outcomes, our findings highlight moderate- to high-quality evidence showing either a significant effect or no significant difference between telerehabilitation and other interventions. There was insufficient evidence to draw a conclusion regarding feasibility outcomes, including participant satisfaction, adherence to treatment, and cost. Most reviews under this umbrella included patients with stroke in the subacute or chronic phase (12 SRs). Simple and complex telerehabilitation interventions such as telephone calls, videoconferencing, smartphone- or tablet-based mobile health applications, messaging, virtual reality, robot-assisted devices, and 3D animation videos, either alone or in combination with other interventions, were included across reviews. Conclusions: Various telerehabilitation interventions have shown either a significant effect or no significant difference compared to other interventions in improving upper and lower limb motor function, balance, gait, ADLs, and QoL, regardless of whether simple or complex approaches were used. Further research is needed to support the delivery of rehabilitation services through telerehabilitation intervention following a stroke. Full article
(This article belongs to the Section Clinical Rehabilitation)
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<p>PRISMA flowchart of study selection and inclusion process. ** Records that were excluded after title and abstract screening.</p>
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23 pages, 748 KiB  
Article
Myotonia Congenita in Australian Merino Sheep with a Missense Variant in CLCN1
by Leah K. Manning, Katie L. M. Eager, Cali E. Willet, Shaun Slattery, Justine H. McNally, Zoe B. Spiers, Mark Hazelton, Georgina Child, Rick Duggan, Brendon A. O’Rourke and Imke Tammen
Animals 2024, 14(24), 3703; https://doi.org/10.3390/ani14243703 - 22 Dec 2024
Viewed by 405
Abstract
Myotonia congenita is a hereditary, non-dystrophic skeletal muscle disorder associated with muscle stiffness due to delayed muscle relaxation after contraction. We review myotonia congenita in domesticated animals and humans and investigated suspected myotonia congenita in a flock of Merino sheep in Australia. In [...] Read more.
Myotonia congenita is a hereditary, non-dystrophic skeletal muscle disorder associated with muscle stiffness due to delayed muscle relaxation after contraction. We review myotonia congenita in domesticated animals and humans and investigated suspected myotonia congenita in a flock of Merino sheep in Australia. In 2020, a property in New South Wales reported a four-year history of lambs that would fall on disturbance before rapidly recovering, with 13 affected sheep identified in 2020. Episodes were associated with a short period of tetanic spasms and a stiff gait upon rising. Lambs were otherwise normal between episodes, although over time, lost body condition and occasionally died from misadventure. An inherited condition was considered from limited pedigree information and a preliminary diagnosis of myotonia congenita was made based on clinical presentation. Biochemistry from four sheep found variable, but typically mild increases in creatine kinase (CK) and aspartate aminotransferase (AST). Modified electromyography on six affected sheep found irregular electrical activity within the muscle. For four sheep, there were no consistent significant abnormalities on post mortem examination and histopathology—typical for this condition. A review of the Online Mendelian Inheritance in Man (OMIM) and Online Mendelian Inheritance in Animals (OMIA) databases was conducted to summarise information about myotonia congenita in humans and eight non-human species of animals. Comparing the characteristic clinical presentation, pathology and electromyography data of affected Merino sheep to similar conditions in other species assisted the identification of likely candidate genes. Whole genome sequencing of two affected lambs detected a missense variant in CLCN1 (NC_056057.1:g.107930611C>T; XM_004008136.5:c.844C>T; XP_004008185.4:p.(P282S)), with a predicted deleterious effect on protein function. An SNP genotyping assay was developed, and the variant segregated with the disease in 12 affected sheep and obligate carrier rams under an assumed recessive mode of inheritance. Identifying a likely causal variant and developing a diagnostic test allows screening of suspected affected or carrier Merino sheep for early intervention to reduce propagation of the variant within flocks. Full article
(This article belongs to the Section Small Ruminants)
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<p>Manhattan plot of a basic case/control GWAS analysis associated with myotonia phenotype. Alternating yellow and blue dots indicate different chromosomes. The <span class="html-italic">p</span>-values obtained from GWAS are presented on −log10 scale. The blue line represents a suggestive significance threshold (−log10(<span class="html-italic">p</span>) = 5) and the red line represents the genome-wide significance threshold (−log10(<span class="html-italic">p</span>) = 8). The peak on ovine chromosome 4 overlaps with the position of the functional candidate gene CLCN1.</p>
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13 pages, 6856 KiB  
Article
Mind the Step: An Artificial Intelligence-Based Monitoring Platform for Animal Welfare
by Andrea Michielon, Paolo Litta, Francesca Bonelli, Gregorio Don, Stefano Farisè, Diana Giannuzzi, Marco Milanesi, Daniele Pietrucci, Angelica Vezzoli, Alessio Cecchinato, Giovanni Chillemi, Luigi Gallo, Marcello Mele and Cesare Furlanello
Sensors 2024, 24(24), 8042; https://doi.org/10.3390/s24248042 - 17 Dec 2024
Viewed by 513
Abstract
We present an artificial intelligence (AI)-enhanced monitoring framework designed to assist personnel in evaluating and maintaining animal welfare using a modular architecture. This framework integrates multiple deep learning models to automatically compute metrics relevant to assessing animal well-being. Using deep learning for AI-based [...] Read more.
We present an artificial intelligence (AI)-enhanced monitoring framework designed to assist personnel in evaluating and maintaining animal welfare using a modular architecture. This framework integrates multiple deep learning models to automatically compute metrics relevant to assessing animal well-being. Using deep learning for AI-based vision adapted from industrial applications and human behavioral analysis, the framework includes modules for markerless animal identification and health status assessment (e.g., locomotion score and body condition score). Methods for behavioral analysis are also included to evaluate how nutritional and rearing conditions impact behaviors. These models are initially trained on public datasets and then fine-tuned on original data. We demonstrate the approach through two use cases: a health monitoring system for dairy cattle and a piglet behavior analysis system. The results indicate that scalable deep learning and edge computing solutions can support precision livestock farming by automating welfare assessments and enabling timely, data-driven interventions. Full article
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<p>The dairy cattle two-camera setup. The side-view camera is used for the estimation of pose and locomotion features, while the top-view camera supports markerless identification.</p>
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<p>Architecture of the AI health status assessment system.</p>
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<p>Ordered list of pose key points and an annotation example over a training set image.</p>
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<p>Structure of the locomotion score classifier. The process starts with a clip, divided into a sequence of frames (<b>left</b>), which are then processed into a sequence of meaningful features (<b>middle</b>). These features are evaluated by the classifier, resulting in a locomotion score prediction (<b>right</b>).</p>
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<p>Structure of the body condition score classifier. The process begins with a snapshot, from which the subject is extracted using a detection model. The extracted subject is then evaluated by the body condition score classifier, producing an estimated BCS value.</p>
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<p>Structure of the markerless identification algorithm. The process starts with a snapshot, where the subject is extracted using a detection model (<b>left</b>). The extracted subject is then encoded into a latent space via an encoder network (<b>middle</b>). Finally, the closest anchor point is identified using the Euclidean distance (<b>right</b>).</p>
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<p>Outline of the pipeline for behavioral analysis.</p>
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<p>(<b>A</b>) Example of how perspective transformation is applied to map pixel coordinates to real-world coordinates, aimed at achieving more precise quantification and characterization of traveled distances. (<b>B</b>) Example of body-part segmentation, with the five segmentation classes (mouth, right ear, left ear, body, and tail) highlighted in different colors and labeled accordingly.</p>
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<p>The pig interactions pipeline. (<b>A</b>) Inference annotations; (<b>B</b>) Daily interaction distribution for Week 1 in Pen 1: total interaction time (minutes) per pig per hour; (<b>C</b>) Classification of interactions based on body-part segmentation; (<b>D</b>) Example of quantitative analysis of an interaction.</p>
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<p>Ear tag-based and livestock mark-based identification systems.</p>
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<p>Visual comparison of different instances (with the two on the left from in-field acquisitions, and the one on the right from the NWAFU dataset [<a href="#B9-sensors-24-08042" class="html-bibr">9</a>]), with the annotated bounding boxes shown in green, and the predicted bounding boxes in purple.</p>
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<p>Visual comparison of different instances (with the two on the left from in-field acquisitions, and the one on the right from the NWAFU dataset [<a href="#B9-sensors-24-08042" class="html-bibr">9</a>]), with the annotated key points shown in green, the predicted key points shown in purple, and the distance between the predicted coordinates and their ground-truth values highlighted in red.</p>
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17 pages, 274 KiB  
Case Report
Witnessing Self-Affirming Moments in Persons with Dementia While Interacting with Therapy Dogs: A Case Report
by Carolyn Brooks, Colleen A. Dell, Darlene Chalmers and Ben D. B. Carey
Animals 2024, 14(24), 3620; https://doi.org/10.3390/ani14243620 - 15 Dec 2024
Viewed by 639
Abstract
Individuals, families, and communities are impacted by Alzheimer’s disease and other dementias worldwide. In Canada and elsewhere, family members commonly see loved ones living with dementia experience difficult moments, including anxiety, stress, and fear. Struggling health care systems strive to apply the latest [...] Read more.
Individuals, families, and communities are impacted by Alzheimer’s disease and other dementias worldwide. In Canada and elsewhere, family members commonly see loved ones living with dementia experience difficult moments, including anxiety, stress, and fear. Struggling health care systems strive to apply the latest evidence-based interventions. In this case report, we explore how individuals who care for people with dementia can help redirect difficult personal and interpersonal moments towards hopeful moments in the presence of therapy dogs. The cases of four individuals diagnosed with dementia and interacting with therapy dog intervention in one of three environments—at a family home, in a hospital awaiting placement to a long-term care facility, and in a psychiatric centre—are reviewed from the perspectives of two attending therapy dog handlers. In addition to being researchers in the therapy dog field and authors on this case report, both handlers were guided in a 110 min focused conversational method by a third author, from which self-affirmation theory surfaced as a guiding framework for explaining their observations and experiences. Therapy dog visiting appears to facilitate positive social interactions that can help redirect difficult moments to moments defined by self-affirmation or experiencing a positive sense of self. This is an under-recognized association in the dementia literature. Self-affirmation is demonstrated through four themes during therapy dog visits: (a) physical movement, (b) psychological shift, (c) a transition in the experience of time, and (d) connective interactions. Therapy dogs are identified here as a promising self-affirming psychosocial intervention for individuals with dementia and their caregivers worthy of further research and practice attention. Full article
(This article belongs to the Section Human-Animal Interactions, Animal Behaviour and Emotion)
26 pages, 1207 KiB  
Article
Behavior Coding of Adolescent and Therapy Dog Interactions During a Social Stress Task
by Seana Dowling-Guyer, Katie Dabney, Elizabeth A. R. Robertson and Megan K. Mueller
Vet. Sci. 2024, 11(12), 644; https://doi.org/10.3390/vetsci11120644 - 12 Dec 2024
Viewed by 414
Abstract
Youth mental health interventions incorporating trained therapy animals are increasingly popular, but more research is needed to understand the specific interactive behaviors between participants and therapy dogs. Understanding the role of these interactive behaviors is important for supporting both intervention efficacy and animal [...] Read more.
Youth mental health interventions incorporating trained therapy animals are increasingly popular, but more research is needed to understand the specific interactive behaviors between participants and therapy dogs. Understanding the role of these interactive behaviors is important for supporting both intervention efficacy and animal welfare and well-being. The goal of this study was to develop ethograms to assess interactive behaviors (including both affiliative and stress-related behaviors) of participants and therapy dogs during a social stress task, explore the relationship between human and dog behaviors, and assess how these behaviors may vary between experimental conditions with varying levels of physical contact with the therapy dog. Using video data from a previous experimental study (n = 50 human–therapy dog interactions, n = 25 control group), we successfully developed behavioral ethograms that could be used with a high degree of interrater reliability. Results indicated differences between experimental conditions in dog and human behaviors based on whether participants were interacting with a live or a stuffed dog, and whether they were allowed to touch the dog. These findings suggest that physically interacting with a live dog may be an important feature of these interventions, with participants demonstrating increased positive behaviors such as laughing and smiling in these conditions. Dog behaviors also varied based on whether they were in the touching/petting condition of the study which could indicate reactions to the session and has potential welfare implications for the dogs. Future research should focus on identifying specific patterns of interactive behaviors between dogs and humans that predict anxiolytic outcomes. Full article
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<p>Original experimental conditions and study timeline/tasks.</p>
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<p>Human behaviors by experimental condition (<span class="html-italic">N</span> = 75). Note: * indicates difference of <span class="html-italic">p</span> &lt; .05 between conditions based on mean rank testing, indicated by brackets.</p>
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<p>Dog behaviors by experimental condition (<span class="html-italic">n</span> = 50). Note: * indicates difference of <span class="html-italic">p</span> &lt; .05 between conditions based on mean rank testing.</p>
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22 pages, 695 KiB  
Systematic Review
The Impact of Dog-Assisted Therapy Among Children and Adolescents with Autism Spectrum Disorder: A Systematic Review
by Paula Galvany-López, Manuel Martí-Vilar, Sergio Hidalgo-Fuentes and Javier Cabedo-Peris
Children 2024, 11(12), 1499; https://doi.org/10.3390/children11121499 - 9 Dec 2024
Viewed by 781
Abstract
Background and Objectives: Animal-assisted therapies have been utilized in various profiles to improve people’s quality of life. This systematic review aims to assess the impact of dog-assisted therapies (DAT) on children and adolescents with Autism Spectrum Disorder (ASD). The benefits provided, the feasibility [...] Read more.
Background and Objectives: Animal-assisted therapies have been utilized in various profiles to improve people’s quality of life. This systematic review aims to assess the impact of dog-assisted therapies (DAT) on children and adolescents with Autism Spectrum Disorder (ASD). The benefits provided, the feasibility of implementation, and potential limitations are analysed. Methods: An exhaustive search was carried out in the following databases: Web of Science (WoS), Scopus, ERIC, PubMed, PubPsych, CINAHL, PsycInfo and Dialnet. The procedure was preregistered on PROSPERO. Results: This review included 19 scientific articles. Of these, 84% suggest that DAT has a positive impact on communication and social interaction during the development of children and adolescents diagnosed with ASD. The dog is often perceived as a communication facilitator that serves as a reinforcer, capturing the individual’s attention. Emotional benefits have also been reported, including reducing stereotyped and self-harming behaviours. Additionally, an increase in smiling frequency and self-esteem levels was observed. Conclusions: These findings highlight the potential of DAT as a component of socio-educational intervention for children and adolescents diagnosed with ASD. However, further research is necessary due to the limited sample sizes in the studies reviewed. Full article
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<p>Flowchart regarding PRISMA guidelines.</p>
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13 pages, 708 KiB  
Article
Recruitment Feasibility for a Randomized Controlled Pilot Study of Animal-Assisted Intervention for Hospitalized Older Adults
by Lisa Townsend, Nancy R. Gee, Erika Friedmann, Megan K. Mueller and Sandra B. Barker
J. Ageing Longev. 2024, 4(4), 404-416; https://doi.org/10.3390/jal4040029 - 3 Dec 2024
Viewed by 448
Abstract
This study evaluated the feasibility of recruiting hospitalized older adults into a randomized controlled trial (RCT) comparing an animal-assisted intervention (AAI) involving visits from therapy dogs with an active control (conversation with a volunteer) and treatment as usual (TU) for addressing health and [...] Read more.
This study evaluated the feasibility of recruiting hospitalized older adults into a randomized controlled trial (RCT) comparing an animal-assisted intervention (AAI) involving visits from therapy dogs with an active control (conversation with a volunteer) and treatment as usual (TU) for addressing health and mental health outcomes. Recruitment occurred on acute care hospital units and a long-term physical rehabilitation facility. Feasibility data included the number of recruitment contacts, recruitment rate, and reasons for non-enrollment. The recruitment pool included 1124 patients; the recruitment rate was 4.8% (n = 54). This study explored the difficulties inherent in recruiting medically ill, hospitalized older adults for an RCT of hospital-based therapy dog visits. We believe this is the first RCT of its kind to evaluate the feasibility of recruiting this population for an animal-assisted intervention. Although the intervention was well received, older adults’ illnesses presented significant barriers to study enrollment. Strategies for improving the recruitment of hospitalized older adults for RCTs are discussed. Full article
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<p>CONSORT enrollment flowchart.</p>
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<p>Distribution of Pearson’s standardized residuals for selected older adult intervention studies. * Represents studies with significantly higher or lower enrollment rates.</p>
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16 pages, 1507 KiB  
Article
The Identification of Predominant Faecal Contamination Sources in Water Using Host-Specific Genetic Markers in Water-Stressed Rural Communities of Vhembe District Municipality, South Africa
by Mulalo Mudau, Renay Ngobeni-Nyambi and Maggy Ndombo Benteke Momba
Water 2024, 16(23), 3477; https://doi.org/10.3390/w16233477 - 3 Dec 2024
Viewed by 432
Abstract
It is critical to attribute faecal contamination to its original source in order to assess public health risks and implement effective interventions to mitigate future contamination. This study aimed to identify the primary sources of faecal contamination in water using microbial source tracking [...] Read more.
It is critical to attribute faecal contamination to its original source in order to assess public health risks and implement effective interventions to mitigate future contamination. This study aimed to identify the primary sources of faecal contamination in water using microbial source tracking markers in water-stressed rural communities. A total of 1128 water samples were collected sequentially from the main source (river/borehole) to the households. Six host-specific genetic markers were used to detect faecal contamination in the water samples (BacHum and HF183, BacCow, Pig-2-Bac, Cytb and BacCan). Of the 564 water samples tested during the wet season, 37.94% (n = 214) were positive for human and animal-specific Bacteroidales marker genes, while 31.73% (n = 179) of the 564 tested during the dry season were also positive. During the wet season, animal faecal contamination was more prevalent among the positive samples (Cytb: 20.56%, n = 44; BacCan: 19.16%, n = 41). By contrast, human-origin faecal contamination was dominant during the dry season (BacHum: 23.46%, n = 42; HF183: 21.23%, n = 38). Identifying the origin of faecal contamination will assist in implementing targeted intervention strategies for the effective prevention of pathogen transmission in water-stressed rural communities in order to protect public health. Full article
(This article belongs to the Section Urban Water Management)
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<p>Map representing villages selected in the Vhembe District Municipality, Limpopo Province.</p>
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<p>Prevalence of sources of faecal contamination in water per season (n = 564 per season).</p>
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<p>(<b>A</b>) The overall prevalence of sources of contamination in different villages. (<b>B</b>) The prevalence of different sources of contamination in different villages during the wet and dry seasons.</p>
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<p>(<b>A</b>) The overall prevalence of sources of contamination in different villages. (<b>B</b>) The prevalence of different sources of contamination in different villages during the wet and dry seasons.</p>
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23 pages, 4820 KiB  
Review
Animals as Architects: Building the Future of Technology-Supported Rehabilitation with Biomimetic Principles
by Bruno Bonnechère
Biomimetics 2024, 9(12), 723; https://doi.org/10.3390/biomimetics9120723 - 22 Nov 2024
Viewed by 802
Abstract
Rehabilitation science has evolved significantly with the integration of technology-supported interventions, offering objective assessments, personalized programs, and real-time feedback for patients. Despite these advances, challenges remain in fully addressing the complexities of human recovery through the rehabilitation process. Over the last few years, [...] Read more.
Rehabilitation science has evolved significantly with the integration of technology-supported interventions, offering objective assessments, personalized programs, and real-time feedback for patients. Despite these advances, challenges remain in fully addressing the complexities of human recovery through the rehabilitation process. Over the last few years, there has been a growing interest in the application of biomimetics to inspire technological innovation. This review explores the application of biomimetic principles in rehabilitation technologies, focusing on the use of animal models to help the design of assistive devices such as robotic exoskeletons, prosthetics, and wearable sensors. Animal locomotion studies have, for example, inspired energy-efficient exoskeletons that mimic natural gait, while insights from neural plasticity research in species like zebrafish and axolotls are advancing regenerative medicine and rehabilitation techniques. Sensory systems in animals, such as the lateral line in fish, have also led to the development of wearable sensors that provide real-time feedback for motor learning. By integrating biomimetic approaches, rehabilitation technologies can better adapt to patient needs, ultimately improving functional outcomes. As the field advances, challenges related to translating animal research to human applications, ethical considerations, and technical barriers must be addressed to unlock the full potential of biomimetic rehabilitation. Full article
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<p>Horse running by Muybridge Eadweard. Credit: Wellcome Collection gallery (<a href="https://openartimages.com/search/eadweard-muybridge" target="_blank">https://openartimages.com/search/eadweard-muybridge</a> (accessed on 22 March 2018)), CC-BY-4.0.</p>
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<p>A man walking. Photogravure after Eadweard Muybridge, 1887. Credit: Wellcome Collection gallery (<a href="https://openartimages.com/search/eadweard-muybridge" target="_blank">https://openartimages.com/search/eadweard-muybridge</a> (accessed on 22 March 2018)), CC-BY-4.0.</p>
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<p>Example of animals inspiring research in technology-supported rehabilitation. (<b>A</b>) Mice for motor recovery; (<b>B</b>) cats for robotic assisted gait training; (<b>C</b>) pigs for post-injury muscular recovery; (<b>D</b>) macaques for cognitive training; (<b>E</b>) rabbits for tendinitis and tendon recovery; (<b>F</b>) dogs for physical therapy techniques; (<b>G</b>) zebrafish for nerve regeneration; (<b>H</b>) sheep for osteoarthritis; (<b>I</b>) rats for nerve recovery and sensorimotor function (Photos from Freepik <a href="https://www.freepik.com/" target="_blank">https://www.freepik.com/</a> (accessed on 10 November 2024)).</p>
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<p>Comparative illustration of elastic and mechanical force models in animal locomotion applied to soft exoskeleton. Top figures show examples of animal movement dynamics: a kangaroo (<b>left</b>) illustrating the role of elastic energy storage and release during hopping, and horses (<b>right</b>) showcasing controlled movement dynamics with muscle forces. Bottom figures represent the simplified biomechanical model of joint forces and elastic components at different stages of the gait cycle, specifically highlighting the knee and ankle forces. During initial contact, the spring (representing elastic tissue) is relaxed as ground reaction forces are absorbed. In mid stance, the spring maintains its original state, supporting joint stability. Terminal stance shows a stretched spring, maximizing energy storage in preparation for the push-off phase. In pre-swing, the spring contracts, converting stored energy into propulsion. Arrows indicate the directions of knee and ankle forces applied during each phase, depicting the interaction between muscle forces and elastic tissue response (Photos from Freepik <a href="https://www.freepik.com/" target="_blank">https://www.freepik.com/</a> (accessed on 10 November 2024)).</p>
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11 pages, 1051 KiB  
Communication
A Retrospective Report of Carprofen Administration as Post-Operative Analgesia Reveals Negative Effects of Recommended Doses
by Zoë Jäckel, Ahmed Adžemović, Benedikt Kloos, Stefanie Hardung, Rita Sanchez-Brandelik, Philippe Coulon and Ilka Diester
Animals 2024, 14(21), 3157; https://doi.org/10.3390/ani14213157 - 4 Nov 2024
Viewed by 1084
Abstract
Effective pain management in laboratory animals is crucial for both animal welfare and the reliability of scientific research. We retrospectively examined the effects of carprofen as post-operative analgesia in Sprague Dawley rats following stereotactic surgery. Our data indicate that administering carprofen twice daily [...] Read more.
Effective pain management in laboratory animals is crucial for both animal welfare and the reliability of scientific research. We retrospectively examined the effects of carprofen as post-operative analgesia in Sprague Dawley rats following stereotactic surgery. Our data indicate that administering carprofen twice daily (5 mg/kg), as currently recommended by Die Gesellschaft für Versuchstierkunde/Society for Laboratory Animal Science (GV-SOLAS), led to adverse effects such as reduced food and water intake, disrupted fecal excretion, and abdominal bloating consistent with peritonitis. Continued administration exacerbated these symptoms, with post-mortem findings of intestinal obstructions and ulcers. However, when the frequency was reduced to once daily, such adverse symptoms were not observed. These results are based on incidental data collected from various neuroscientific experiments, resulting in small and uneven sample groups across various experimental cohorts. The inherent imbalances in these groups present challenges for statistical interpretation. While the findings suggest that less frequent carprofen use may reduce adverse effects, the surgical interventions and concurrent use of other drugs in these experiments likely exacerbated these outcomes. Further investigation into the interactions between carprofen, surgical stress, and other perioperative factors is needed to refine analgesia protocols in laboratory animals. Despite these limitations, these observations contribute to understanding analgesia protocols and may assist in improving animal welfare practices. Full article
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<p><b>Original analgesia protocol vs. refined analgesia protocol.</b> Schematic comparing the original analgesia protocol with the current refined model for our experimental purposes. The black arrows represent default carprofen administration per the approved timeline; the grey arrows indicate observation-based administration, including emergency treatments for unexpected symptom presentation (see post-operative care section). Both protocols include a minimum of a 3-day post-surgical observation, including a recording of the daily weight, as well as documentation of animal monitoring, including any symptoms of pain or discomfort, along with wet food supplementation; in both protocols, any possible extension of post-OP care is based on individual observations. The left column describes the original analgesia treatment, with carprofen automatically administered twice daily (spread across visits in the morning and evening) for 3 days post-OP. The right column displays the refined analgesia treatment, where carprofen is applied once daily for 2 days. Additional applications of carprofen can be further applied as needed, provided there is a minimum of 12 h between doses, and no gastrointestinal (GI) issues are found. In case of GI issues, carprofen is replaced with metamizole. The refined protocol tailors treatment to precise observations and considers carprofen’s impact on the gastric system. The original post-OP protocol resulted in high incidence of peritonitis-indicative symptoms, while refined post-OP protocols resulted in zero incidence of such symptom presentation.</p>
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<p><b>Rat health status.</b> (<b>a</b>) Frequency of health status, treatment protocol, and sex. Blue shades: low frequency carprofen treatment; red/orange shades: high frequency carprofen treatment; brighter shades: female rats; medium shades: male rats; solid black outline: symptomatic (symp.) rats; no outline: healthy rats. The number of animals in each group is labelled in white text. (<b>b</b>) Box plot visualizing the distribution (median value: center bar; interquartile range: shaded box; minimum and maximum values: whiskers) of body weights of all symptomatic rats vs. all healthy rats (left) and symptomatic rats vs. healthy rats within the high frequency carprofen group. (<b>c</b>) Stacked bar plot visualizing the number of symptomatic and healthy animals per surgery type. The number of each surgery type and health outcome used to analyze the effect of surgery on health status is labelled in white on each bar. The bars are overlaid with a line pattern indicating where the animals underwent high frequency carprofen treatment.</p>
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11 pages, 616 KiB  
Systematic Review
Use of Laughter Therapy for In Vitro Fertilization: A Systematic Review
by Daniela Rodríguez-García, Claudio-Alberto Rodríguez-Suárez and Héctor González-de la Torre
Reprod. Med. 2024, 5(4), 252-262; https://doi.org/10.3390/reprodmed5040022 - 2 Nov 2024
Viewed by 1148
Abstract
Background/Objectives: In vitro fertilization (IVF) is an assisted reproductive technique for women and couples experiencing difficulties in achieving a spontaneous pregnancy, often due to stressors that negatively affect fertility. Humor can be beneficial in these stressful situations, helping to reduce symptoms of anxiety [...] Read more.
Background/Objectives: In vitro fertilization (IVF) is an assisted reproductive technique for women and couples experiencing difficulties in achieving a spontaneous pregnancy, often due to stressors that negatively affect fertility. Humor can be beneficial in these stressful situations, helping to reduce symptoms of anxiety and depression. The primary aim was to analyze the effectiveness of laughter therapy in increasing pregnancy rates in women undergoing IVF. The secondary aims were to identify different types of laughter therapy interventions and evaluate their benefits. Methods: A systematic review was conducted using Medline, Web of Science, Scopus, Cinahl, ProQuest and Lilacs, with the search terms “laughter”, “laughter therapy”, “fertilization in vitro” and “fertilization”. Intervention studies published in English, Spanish, or Portuguese were included, with no limits on the date of publication. Studies with other designs, those conducted with animals and grey literature were excluded. The quality of the included studies was assessed using the Joanna Briggs Institute critical appraisal tools. Results: In total, n = 3 studies (1 randomized clinical trial and 2 quasi-experimental) were included, evaluating pregnancy rates and symptoms of depression and anxiety. The interventions included clowns performing magic tricks and interpreting comic situations. Additionally, they incorporated hand clapping, breathing exercises, childlike playfulness, drinking milk, muscle relaxation exercises, candles, and music. Conclusions: This review does not provide clear evidence on the effectiveness of laughter therapy in increasing pregnancy success in IVF. Although it may have some positive effects in reducing symptoms of depression and anxiety, it is important to note that while the reduction of these symptoms may enhance the emotional well-being of patients, it has not been demonstrated to directly lead to an increase in pregnancy rates. Laughter therapy is an innovative, non-pharmacological intervention that is simple, non-invasive, easy to implement and cost-effective; however, the number of available studies is insufficient. More research is needed to provide better and higher-quality evidence using rigorous designs to evaluate this intervention in IVF clinical practice. Full article
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<p>PRISMA flow diagram (Source: ref. [<a href="#B14-reprodmed-05-00022" class="html-bibr">14</a>]).</p>
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10 pages, 1264 KiB  
Article
Animal-Assisted Interventions: Factors Affecting Donkey Behaviours and Attitude Toward Humans
by Serenella d’Ingeo, Valeria Straziota, Marcello Siniscalchi, Onofrio Depalma, Sara Petrassi, Michela Romano and Angelo Quaranta
Animals 2024, 14(21), 3139; https://doi.org/10.3390/ani14213139 - 1 Nov 2024
Viewed by 1035
Abstract
The relationship between humans and donkeys has ancient origins. In recent years, donkeys’ involvement in Animal-Assisted Intervention (AAI) has grown, raising major concerns about their welfare during these activities. We investigated the factors affecting donkeys’ behaviour during an AAI programme for migrants. We [...] Read more.
The relationship between humans and donkeys has ancient origins. In recent years, donkeys’ involvement in Animal-Assisted Intervention (AAI) has grown, raising major concerns about their welfare during these activities. We investigated the factors affecting donkeys’ behaviour during an AAI programme for migrants. We observed an effect of human distance from the animal, the interaction mode, and food presence on the animals’ behaviours and attitudes toward humans. Moreover, we provide preliminary evidence of the effect of AAI on migrants’ welfare and prosocial behaviours and offer insights into the issues to address in future studies. Full article
(This article belongs to the Special Issue New Insights in Human-Animal Relationship: Emotions and Welfare)
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<p>An interaction between humans and one of the donkeys involved in the project.</p>
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<p>Data of the score of the stress-related behaviour category for each of the distances between the humans and animals considered (means with SEM are shown). ***: <span class="html-italic">p</span> &lt; 0.001.</p>
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13 pages, 570 KiB  
Article
I Prefer to Look at an Animal Rather than at a Human: Visual Attention of Neurotypical Children and Children with Autism Spectrum Disorder (ASD) During One-Time First Exposure to an Assistance Dog
by Manon Toutain, Marine Malivoir, Pauline Brugaillères, Inès Tiercelin, Carole Jacq, Yentl Gautier, Camille Cagnot, Albane Péchard, Ronan Jubin, Laurence Henry, Martine Hausberger and Marine Grandgeorge
Pets 2024, 1(3), 315-327; https://doi.org/10.3390/pets1030022 - 25 Oct 2024
Viewed by 663
Abstract
Interacting with animals often provides numerous benefits for children with autism spectrum disorder (ASD). One potential explanation for this is that children with ASD exhibit particular visual attention to animals. This study aimed to characterize the visual attention patterns towards humans and animals [...] Read more.
Interacting with animals often provides numerous benefits for children with autism spectrum disorder (ASD). One potential explanation for this is that children with ASD exhibit particular visual attention to animals. This study aimed to characterize the visual attention patterns towards humans and animals in children with ASD compared to neurotypical (NT) children during one-time first exposure to an assistance dog. Forty children participated (18 ASD, 22 NT). The interventions were videorecorded. Three categories of visual attention (gaze, glance, and mutual gaze) directed towards assistance dogs and human professionals were coded using ethological methods. Both the children with ASD and the NT children gazed more frequently at the assistance dog than at the professional. However, although infrequently, both groups shared more mutual gazes with the professional than with the assistance dog. Both the NT children and the children with ASD preferred to gaze rather than to glance at, or share mutual gazes with, the assistance dogs. The attention patterns of the NT children towards the professionals were consistent, whereas those of the children with ASD were not. Statistical linear models revealed that a child’s status (ASD/NT) was the only factor influencing the frequency and duration of the visual behaviors observed. These results confirm that individuals with ASD exhibit particularly focused attention towards animals. Full article
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<p>Visual attention of (<b>A</b>) the autism spectrum disorders (ASD) and (<b>B</b>) neurotypical (NT) groups of children in occurrence per minute for glances, gazes, and mutual gazes. Wilcoxon test. * <span class="html-italic">p</span> &lt; 0.05, *** <span class="html-italic">p</span> ≤ 0.001.</p>
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21 pages, 357 KiB  
Article
The Experiences of Motor Skill Development in Children with Autism Spectrum Disorder (ASD) Reflected through Parental Responses
by Anetta Müller, Éva Bácsné Bába, Peter Židek, Attila Lengyel, Judit Herpainé Lakó, Nóra Laoues-Czimbalmos, Anikó Molnár and Eszter Boda
Children 2024, 11(10), 1238; https://doi.org/10.3390/children11101238 - 14 Oct 2024
Viewed by 1509
Abstract
Background/Objectives: Understanding movement development in children with autism spectrum disorder (ASD) is critical for creating effective intervention strategies. This study aims to explore parental perspectives on the movement development of children with ASD, focusing on identifying common challenges and successful strategies. The [...] Read more.
Background/Objectives: Understanding movement development in children with autism spectrum disorder (ASD) is critical for creating effective intervention strategies. This study aims to explore parental perspectives on the movement development of children with ASD, focusing on identifying common challenges and successful strategies. The objective is to analyze the experiences of parents to highlight the most effective approaches to support motor, communication, and social skills development in these children. Methods: Using a qualitative approach, we conducted in-depth interviews with parents of children with ASD. The data were analyzed using open and selective coding to identify key themes related to movement development challenges and strategies. The analysis included cross-referencing with the existing literature to support parental insights. Results: This study identified several key themes, including communication barriers, social interaction difficulties, and the importance of personalized movement programs. Parents reported significant challenges in understanding and addressing their children’s movement needs, particularly in group settings. However, activities such as water programs, music and dance, and animal-assisted therapies were found to be highly engaging. Motivation was a critical factor, with rewards and active parental involvement enhancing participation. Conclusions: This study highlights the crucial role of a supportive environment, including professional guidance and family support, in the success of movement development programs. Full article
(This article belongs to the Section Pediatric Mental Health)
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