Search Results (9,231)

The BRI1-EMS-SUPPRESSOR 1 (BES1) family comprises plant-specific transcription factors, which are distinguished by atypical bHLH domains. Over the past two decades, genetic and biochemical studies have established that members of the BRI1-EMS-SUPPRESSOR 1 (BES1) family are crucial for regulating the expression of genes involved in brassinosteroid (BR) response in rapeseed. Due to the significance of the BES1 gene family, extensive research has been conducted to investigate its functional properties. This study presents a comprehensive identification and computational analysis of BES1 genes in ‘Tieguanyin’ (TGY) tea (Camellia sinensis). A total of 10 BES1 genes were initially identified in the TGY genome. Through phylogenetic tree analysis, this study uniquely revealed that CsBES1.2 and CsBES1.5 cluster with SlBES1.8 from Solanum lycopersicum, indicating their critical roles in fruit growth and development. Synteny analysis identified 20 syntenic genes, suggesting the conservation of their evolutionary functions. Analysis of the promoter regions revealed two types of light-responsive cis-elements, with CsBES1.4 exhibiting the highest number of light-related cis-elements (13), followed by CsBES1.9 and CsBES1.10. Additional validation via qRT-PCR experiments showed that CsBES1.9 and CsBES1.10 were significantly upregulated under light exposure, with CsBES1.10 reaching approximately six times the expression level of the control after 4 h. These results suggest that CsBES1.9 and CsBES1.4 could play crucial roles in responding to abiotic stress. This study offers novel insights into the functional roles of the BES1 gene family in ‘Tieguanyin’ tea and establishes a significant foundation for future research, especially in exploring the roles of these genes in response to abiotic stresses, such as light exposure. Full article

Transformers have emerged as the central backbone architecture for modern generative AI. However, most ML applications targeting low-power, low-cost SoCs (TinyML apps) do not employ Transformers as these models are thought to be challenging to quantize and deploy on small devices. This work proposes a methodology to reduce Transformer dimensions with an extensive pruning search. We exploit the intrinsic redundancy of these models to fit them on resource-constrained devices with a well-controlled accuracy tradeoff. We then propose an optimized library to deploy the reduced models using BFLoat16 with no accuracy loss on Commercial Off-The-Shelf (COTS) RISC-V multi-core micro-controllers, enabling the execution of these models at the extreme edge, without the need for complex and accuracy-critical quantization schemes. Our solution achieves up to $220\times$ speedup with respect to a naïve C port of the Multi-Head Self Attention PyTorch kernel: we reduced MobileBert and TinyViT memory footprint up to ∼94% and ∼57%, respectively, and we deployed a tinyLLAMA SLM on microcontroller, achieving a throughput of 1219 tokens/s with an average power of just 57 mW. Full article

Due to the continuous growth of global carbon dioxide emissions, the development of cost-effective carbon dioxide capture technology has attracted extensive attention. Amino-modified chitosan aerogels with lamellar porous structures are good candidates as carbon dioxide adsorbents because of their degradable properties and low energy consumption. Polyethylene polyamine-modified chitosan aerogels (PEPA-CSs) were prepared through a process of crosslinking and freeze-drying using a chitosan solution, polyethylene polyamine (PEPA), and epichlorohydrin (ECH) as raw materials. The amino group of PEPA was proven to be successfully grafted on the chitosan surface by FITR and XPS. The SEM and TEM analysis showed a rich three-dimensional porous structure and a good rigidity and bearing capacity of the PEPA-CS. The adsorption capacity was significantly increased by PEPA grafting with a maximum value of 1.59 mmol/g at 25 °C and 1 bar through both physical and chemical interactions, which indicates a potential for broad application prospects in industrial CO₂-capture applications. Full article

The detection of glucose is crucial for diagnosing diseases such as diabetes and enables timely medical intervention. In this study, a disposable enzymatic screen-printed electrode electrochemical biosensor enhanced with machine learning (ML) for quantifying glucose in serum is presented. The platinum working surface was modified by chemical adsorption with biographene (BGr) and glucose oxidase, and the enzyme was encapsulated in polydopamine (PDP) by electropolymerisation. Electrochemical characterisation and morphological analysis (scanning and transmission electron microscopy) confirmed the modifications. Calibration curves in Cormay serum (CS) and selectivity tests with chronoamperometry were used to evaluate the biosensor’s performance. Non-linear ML regression algorithms for modelling glucose concentration and calibration parameters were tested to find the best-fit model for accurate predictions. The biosensor with BGr and enzyme encapsulation showed excellent performance with a linear range of 0.75–40 mM, a correlation of 0.988, and a detection limit of 0.078 mM. Of the algorithms tested, the decision tree accurately predicted calibration parameters and achieved a coefficient of determination above 0.9 for most metrics. Multilayer perceptron models effectively predicted glucose concentration with a coefficient of determination of 0.828, demonstrating the synergy of biosensor technology and ML for reliable glucose detection. Full article

Cigarette smoke (CS), an intricate blend comprising over 4000 compounds, induces abnormal cellular reactions that harm multiple tissues. Non-alcoholic fatty liver disease (NAFLD) is a prevalent chronic liver disease (CLD), encompassing non-alcoholic fatty liver (NAFL), non-alcoholic steatohepatitis (NASH), cirrhosis, and hepatocellular carcinoma (HCC). Recently, the term NAFLD has been changed to metabolic dysfunction-associated steatotic liver disease (MASLD), and NASH has been renamed metabolic dysfunction-associated steatohepatitis (MASH). A multitude of experiments have confirmed the association between CS and the incidence and progression of MASLD. However, the specific signaling pathways involved need to be updated with new scientific discoveries. CS exposure can disrupt lipid metabolism, induce inflammation and apoptosis, and stimulate liver fibrosis through multiple signaling pathways that promote the progression of MASLD. Currently, there is no officially approved efficacious pharmaceutical intervention in clinical practice. Therefore, lifestyle modifications have emerged as the primary therapeutic approach for managing MASLD. Smoking cessation and the application of a series of natural ingredients have been shown to ameliorate pathological changes in the liver induced by CS, potentially serving as an effective approach to decelerating MASLD development. This article aims to elucidate the specific signaling pathways through which smoking promotes MASLD, while summarizing the reversal factors identified in recent studies, thereby offering novel insights for future research on and the treatment of MASLD. Full article

Glycerohydrogels based on silicon glycerolate, chitosan (CS) and polyvinyl alcohol (PVA) are widely studied for use in biomedical applications. In line with the general trend of replacing synthetic polymers with natural ones in such compositions, it would be of interest to replace PVA with the polysaccharide glucomannan (GM), as well as to introduce functional additives to impart the desired properties, including gelation time, to the final hydrogel. In this work, a comprehensive study of the preparation conditions and properties of glycerohydrogels based on silicon tetraglycerolate, chitosan hydrochloride (CS·HCl) and GM was carried out. Viscometry was used to assess the conformational state of CS·HCl and GM macromolecules, and their associates in solution before gelation. Gelation was studied using the vessel inversion method. The mucoadhesive and the dermoadhesive properties of the glycerohydrogels obtained were assessed using the tearing off method from the model substrates simulating mucous and dermal tissues. The conformational state of the individual polymers and their mixed associates in solution before gelation was estimated; the intrinsic viscosity and the hydrodynamic radius of the macromolecular coils were calculated. The influence of various factors (addition of ε-aminocaproic and hydrochloric acids, sodium chloride, hydroxide and tetraborate to vary the acidity and ionic strength of the medium, as well as temperature) and the molecular weight of chitosan on the gelation time was studied. The gelation time achieved was less than 2 min, which is promising in practical terms, i.e., for creating liquid plasters. Our best samples are not inferior to the commercial preparation “Metrogyl Denta”^® in terms of tearing force during mucoadhesion and dermoadhesion at short gelation times. Thus, the glycerohydrogels synthesized by us and based on silicon tetraglycerolate, CS·HCl and GM could find usage in new biopharmaceutical and biomedical applications. Full article

The nuclear factor Y (NF-Y) transcription factor is widely involved in various plant biological processes, such as embryogenesis, abscisic acid signaling, and abiotic stress responses. This study presents a comprehensive genome-wide identification and expression profile of transcription factors NF-YB and NF-YC in Pinus koraiensis. Eight NF-YB and seven NF-YC transcription factors were identified through bioinformatics analysis, including sequence alignment, phylogenetic tree construction, and conserved motif analysis. We evaluate the expression patterns of NF-YB/C genes in various tissues and somatic embryo maturation processes through the transcriptomics of ABA-treated tissues from multiple nutritional tissues, reproductive tissues, and somatic embryo maturation processes. The Leafy cotyledon1 (LEC1) gene belongs to the LEC1-type gene in the NF-YB family, numbered PkNF-YB7. In this study, we characterized the function of PkLEC1 during somatic embryonic development using genetic transformation techniques. The results indicate that PkNF-YB/C transcription factors are involved in the growth and development of nutritional tissues and reproductive organs, with specific high expression in PkNF-YB7 embryogenic callus, somatic embryos, zygotic embryos, and macropores. Most PkNF YB/C genes do not respond to ABA treatment during the maturation culture process. Compared with the absence of ABA, PkNF-YB8 was up-regulated in ABA treatment for one week (4.1 times) and two weeks (11.6 times). However, PkNF-YC5 was down-regulated in both one week (0.6 times) and two weeks (0.36 times) of culture, but the down-regulation trend was weakened in tissues treated with ABA (0.72–0.83 times). In addition, the promoter of PkNF YB/Cs was rich in elements that respond to various plant hormones, indicating their critical role in hormone pathways. The overexpression of PkLEC1 stimulated the generation of early somatic embryos from callus tissue with no potential for embryogenesis, enhancing the somatic embryogenesis ability of P. koraiensis callus tissue. Full article

In this work, WO₃/TiO₂ core–shell (C-S) nanowires (NWs) were successfully synthesized by the coaxial electrospinning method and subsequent high-temperature calcination treatment. After some microscopic structural characterizations, although the prepared WO₃–TiO₂ and TiO₂–WO₃ C-S NWs displayed quite different surface morphologies, both of the shell coatings were uniform and their typical shell thicknesses were extremely close, with mean values of 22 and 20 nm, respectively. In gas sensing tests, WO₃/TiO₂ C-S NWs exhibited good selectivity towards triethylamine (TEA) without significant interfering gases. Compared with bare WO₃ and TiO₂ NWs, WO₃/TiO₂ C-S NWs showed better gas sensing performance. Specifically, the optimal operating temperature and response of TiO₂–WO₃ C-S NWs to 100 ppm TEA were 130 °C and 106, which were reduced by 70 °C and increased by 5.73 times compared to bare WO₃, respectively. Obviously, the C-S nanostructures contributed to improving the gas sensing performance of materials towards TEA. Finally, some hypothetical sensing mechanisms were proposed, which were expected to have important reference significance for the design of target products applied to TEA sensing. Full article

In recent years, there has been significant investigation into the high efficiency of perovskite solar cells. These cells have the capacity to attain efficiencies above 14%. As the perovskite materials that include lead pose a substantial environmental risk, components that are free from lead are used during the process of solar cell development. In this work, we use a lead-free double-perovskite material, namely Cs₂TiBr₆, as the main absorbing layer in perovskite solar cells to enhance power conversion efficiency (PCE). This work is centered on the development of solar cell structures with materials such as an ETL (electron transport layer) and an HTL (hole transport layer) to enhance the PCE. In this theoretical work, we perform simulations and analysis on double-perovskite Cs₂TiBr₆ to assess its efficacy as an absorber material in various HTLs like Cu₂O and CuI, with a fixed ETL of C60 using SCAPS (Solar Cell Capacitance Simulator, SCAPS 3.3.10) Software. This is a one-dimensional solar cell simulation program. In this work, the thickness of the double-perovskite material is also varied between 0.2 and 2.0 µm, and its efficiency is observed. The effect of temperature variation on efficiency in the range of 300 K to 350 K is observed. The effect of defect density on efficiency is also observed in the range of 1 × 10¹¹ to 1 × 10¹⁶. In this theoretical work, perovskite solar cells, including their absorbing layer, demonstrate outstanding ETLs and HTLs, respectively. As a result, the cells’ achieved PCE is improved. This work demonstrates the effectiveness of this lead-free double-perovskite structure that absorbs light in perovskite solar cells. Full article

Background and Objectives: We aimed to evaluate the current uterotonic administration practices among anesthesiologists and obstetricians and gynecologists (OBGYNs) during cesarean section (CS), focusing on variations in approaches for low- and high-risk postpartum hemorrhage (PPH) cases. The objective was to identify key differences and provide evidence that could contribute to the development of standardized national protocols for uterotonic usage. Materials and Methods: A snapshot online survey was employed between October 2021 and January 2022 and distributed to anesthesiologists and OBGYNs from university-affiliated, government, and private hospitals across Turkey, consisting of 23 questions addressing demographic data, institutional PPH rates, first-line uterotonic choices, administration methods, and dose adjustments for low- and high-risk PPH cases. Specific questions also targeted uterotonic usage in the presence of comorbidities such as pre-eclampsia and cardiac disease. Results: There were 204 responses (54% anesthesiologists and 46% OBGYNs) out of 220, yielding a response rate of 92.7%. Oxytocin was the most common first-line uterotonic for CS with low-risk PPH (99.1% of the anesthesiologists and 96.8% of the OBGYNs). In total, 60% of the anesthesiologists favored an intravenous (IV) bolus followed by infusion, while 56.4% of the OBGYNs preferred IV infusion alone (p < 0.001). For CS with high-risk PPH, approximately half of the participants reported increases in oxytocin dose, while 26.4% of the anesthesiologists and 20.2% of the OBGYNs opted for combined oxytocin and carbetocin use. During intrapartum CS, 69.1% of anesthesiologists and 77.7% of OBGYNs reported no change in dose. However, 11.8% of the anesthesiologists indicated combining oxytocin and carbetocin (p < 0.05). In managing pre-eclampsia and cardiac disease, the anesthesiologists were likely to reduce uterotonic doses (15.5%) and avoid methylergonovine (35.5%) compared to the OBGYNs, who reduced doses less frequently (4.3%), but 79.8% of the OBGYNs avoided methylergonovine (p < 0.001). Conclusions: There was considerable variability in uterotonic administration practices between the anesthesiologists and OBGYNs. Full article

 Cigarette smoke (CS) is a driver of many respiratory diseases, including chronic obstructive pulmonary disease (COPD) and non-small cell lung cancer (NSCLC). Tobacco causes oxidative stress, impaired phagocytosis of alveolar macrophages (AMs), and alterations in gene expression in the lungs of smokers. MicroRNAs (miRNAs) are small non-coding RNAs that influence several regulatory pathways. Previously, we monitored the expressions of hsa-miR-223-5p, 16-5p, 20a-5p, -17-5p, 34a-5p, and 106a-5p in AMs derived from the bronchoalveolar lavage (BAL) of subjects with NSCLC, COPD, and smoker and non-smoker control groups. Here, we investigated the capability of CS conditionate media to modulate the abovementioned miRNAs in primary AMs obtained in the same 43 sex-matched subjects. The expressions of has-miR-34a-5p, 17-5p, 16-5p, 106a-5p, 223-5p, and 20a-5p were assessed before and after in vitro CS exposure by RT-PCR. In addition, a comprehensive bioinformatic analysis of miRNAs KEGGS and PPI linked to inflammation was performed. Distinct and common miRNA expression profiles were identified in response to CS, suggesting their possible role in smoking-related diseases. It is worth noting that, following exposure to CS, the expression levels of hsa-miR-34a-5p and 17-5p in both smokers and non-smokers, 106a-5p in non-smokers, and 20a-5p in smokers, shifted towards those found in individuals with COPD, suggesting them as a risk factor in developing this lung condition. Moreover, CS-focused sub-analysis identified miRNA which exhibited CS-dependent pattern and modulated mRNA involved in the immune system or AMs property regulation. In conclusion, our study uncovered miRNA signatures in AMs exposed to CS, indicating that CS might modify epigenetic patterns that contribute to macrophage activation and lung disease onset and progression. Full article

Joint bearings are widely used in modern industry in order to improve the mechanical properties of the outer surface of its inner ring. A laser quenching experiment was carried out in this paper. First of all, an experimental investigation was conducted on GCr15 ball-bearing material utilizing laser quenching, focusing on the effects of laser irradiation angles ranging from 0° to 10° and laser power levels between 600 W and 1100 W on the degree of hardening and microstructural alterations of the bearing material. Additionally, a reliable finite element analysis model was developed to assess the temperature field throughout the process. The findings indicate that an inclined laser enhances the stability of the hardened layer. Specifically, the hardening effect is minimal when the laser power is below 700 W, and optimal hardening is observed at power levels between 800 W and 900 W. During the laser quenching process when the temperature of the bearing material surpasses Ac1, the cooling rate can exceed 1700 °C/s. In regions where the peak temperature exceeds Ac1, the microstructure will undergo refinement, resulting in a reduction in the size of the martensite and a significant decrease in the number of carbides. In addition, the hardness value of these regions can be increased by 6 to 8 HRC, and the thickness of the quenching layer may exceed 0.3 mm. In the temperature range between Ac1 and Ms, the bearing material undergoes tempering, resulting in lower hardness compared to the base material, along with larger martensite and carbide particles. Furthermore, when using the overlap technique during the laser quenching, there will be a tempering zone both inside and on the surface of the bearing; meanwhile, the heat zones generated by different passes of the laser may have partly interacted, and the hardened zone generated by the previous pass may undergo tempering again. Full article

Tieguanyin tea, a renowned oolong tea, is one of the ten most famous teas in China. The Squamosa Promoter Binding Protein (SBP)-box transcription factor family, widely present in plants, plays a crucial role in plant development, growth, and stress responses. In this study, we identify and analyze 22 CsSBP genes at the genome-wide level. These genes were distributed unevenly across 11 chromosomes. Using Arabidopsis thaliana and Solanum lycopersicum L. as model organisms, we constructed a phylogenetic tree to classify these genes into six distinct subfamilies. Collinearity analysis revealed 20 homologous gene pairs between AtSBP and CsSBP, 21 pairs between SiSBP and CsSBP, and 14 pairs between OsSBP and CsSBP. Cis-acting element analysis indicated that light-responsive elements were the most abundant among the CsSBP genes. Protein motif, domain, and gene architecture analyses demonstrated that members of the same subgroup shared similar exon–intron structures and motif arrangements. Furthermore, we evaluated the expression profiles of nine CsSBP genes under light, shade, and cold stress using qRT-PCR analysis. Notably, CsSBP1, CsSBP17, and CsSBP19 were significantly upregulated under all three stresses. This study provides fundamental insights into the CsSBP gene family and offers a novel perspective on the mechanisms of SBP transcription factor-mediated stress responses, as well as Tieguanyin tea’s adaptation to environmental variations. Full article

Entrepreneurship has been critical in fostering economic growth. The technological innovations and quality of institutions are crucial in promoting entrepreneurship and promoting an environment conducive to entrepreneurial activities. This study investigated the effect of technological innovations and institutional quality on entrepreneurial development with annual data from 2014 to 2021 across Southeastern European countries. The cross-sectional auto-regressive regressive distributed lag model (C-S ARDL), quantile regression and Granger causality were employed to achieve the objectives of this study. A dynamic panel generalized method of moments (GMM) estimator was also applied to perform a robust analysis. The findings revealed a significant long-term relationship between technological innovations and entrepreneurial development, with a coefficient of 0.088. There also exists a significant and positive impact on institutional quality and entrepreneurial development in the long run, with a coefficient of 5.912. Furthermore, the outcome revealed that the exchange rate negatively influences entrepreneurial development in Southeast Europe. The Granger causality reports a bi-directional relationship between technological innovations and entrepreneurial development in Southeastern Europe. The study concluded that a significant relationship exists between technological innovations, institutional quality, and entrepreneurial development in Southeastern Europe. The study recommends that governments of Southeastern European countries strengthen their regulatory structures and institutions to improve the welfare of society through a reduction in political, social, and economic unpredictability while boosting trust and investment from entrepreneurs. Full article

The effect of exogenous mixed-enzyme supplementation (xylanase, β-glucanase, and pectinase) and coccidia vaccine challenge (CVC, Coccivac B-52™) on broilers fed a corn–SBM (CS) and a wheat–CS (WCS)-based diet was examined in this study. On day 14, 448 Cobb by-product breeder male broiler chickens were assigned to treatments (factorial arrangement) in a completely randomized design, with each treatment replicated seven times. Treatment effect was evaluated within each diet type as a 2 (enzyme levels) x 2 (CVC, 0 or 20X) factorial arrangement of treatments 7 and 14 days post-CVC. The 7-day (days 14–21) post-CVC, BWG, and feed efficiency (birds on the CS-based diet) were lower (p < 0.05), while birds on enzyme-supplemented diets had higher (p < 0.05) BWG compared to birds on diets without enzyme supplementation. Between days 21 and 28, an interaction between CVC and exogenous enzyme resulted in higher (p < 0.05) BWG compared with the challenged birds fed diets without enzyme supplementation. For birds fed WCS-based diets, CVC influenced (p < 0.05) BWG and feed efficiency (decreased days 14–21 and increased days 21–28), while CVC birds had higher BWG and feed efficiency 14 days post-CVC. Apparent ileal digestibility of dry matter, energy, and DE were lower (p < 0.05) in CVC broilers fed either the CS- or WCS-based diets (7 and 14 days post-CVC). Interaction between CVC and exogenous enzyme supplementation indicated that CVC, irrespective of exogenous enzyme supplementation with the WCS-based diet, decreased (p < 0.05) Ca utilization (7 days post-CVC) but increased (p < 0.05) Ca utilization compared to CVC birds without enzyme supplementation 14 days post-challenge. Seven days post-CVC, irrespective of the diet type, CVC resulted in lower (p < 0.05) duodenal VH and VH:CD and higher (p < 0.05) CD. Enzyme supplementation influenced (p < 0.05) duodenal CD (increased) and VH:CD (decreased) in birds fed the WCS-based diet. Results from this study showed that complete recovery from CVC was influenced by diet type, with CVC birds fed WCS-based diet having higher BWG and feed efficiency compared to the unchallenged birds. Full article