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Search Results (909)

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Journal = Animals
Section = Animal System and Management

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13 pages, 701 KiB  
Article
Effects of Precision Feeding on Economic and Productive Yields of Hyperprolific Lactating Sows Allocated at Different Farrowing Pens
by María Aparicio-Arnay, Natalia Yeste-Vizcaíno, Nerea Soria, Jorge Cambra, Beatriz Isabel, Carlos Piñeiro and Antonio Gonzalez-Bulnes
Animals 2025, 15(5), 763; https://doi.org/10.3390/ani15050763 - 6 Mar 2025
Viewed by 84
Abstract
The results obtained using precision feeding in maternities (electronic sow feeders, ESFs) may be affected by the type of farrowing pen, as the design of the pen may modify the behavior of the sows. Hence, the aim of the present study was to [...] Read more.
The results obtained using precision feeding in maternities (electronic sow feeders, ESFs) may be affected by the type of farrowing pen, as the design of the pen may modify the behavior of the sows. Hence, the aim of the present study was to determine the effects and possible interactions of electronic feeding systems in different types of farrowing pens (traditional, socialization and loose housing pens) on the productive and economic yields of lactating hyperprolific sows under commercial farm conditions. The results showed an overall lower feed disappearance in sows fed with ESFs and, consequently, a lower amount of feed per weaned piglet and per kg of weaned piglet, with evidence of improved welfare and no negative effects on body weight, condition at weaning or reproductive yields in the following cycle. These effects were, however, modulated by the type of pen and the type of bawl feeder; this finding highlights the need for improvement in the design of facilities and especially feeders to allow the sow a better use of available feed. Full article
(This article belongs to the Section Animal System and Management)
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<p>Schematic representation of the groups studied, with 12 sows fed with either electronic sow feeders (ESF group) or traditional feeders (CON group) in three different housing systems (traditional crates, TCs; social crates, SCs; and loose housing, LH; upper figure). Pictures of the bowl feeders are represented in the lower figure. Half of the sows in both the ESF and CON groups in the LH housing had bowl feeders with or without drainage (DR and NODR, respectively).</p>
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15 pages, 2895 KiB  
Article
Classification of FAMACHA© Scores with Support Vector Machine Algorithm from Body Condition Score and Hematological Parameters in Pelibuey Sheep
by Oswaldo Margarito Torres-Chable, Cem Tırınk, Rosa Inés Parra-Cortés, Miguel Ángel Gastelum Delgado, Ignacio Vázquez Martínez, Armando Gomez-Vazquez, Aldenamar Cruz-Hernandez, Enrique Camacho-Pérez, Dany Alejandro Dzib-Cauich, Uğur Şen, Hacer Tüfekci, Lütfi Bayyurt, Hilal Tozlu Çelik, Ömer Faruk Yılmaz and Alfonso J. Chay-Canul
Animals 2025, 15(5), 737; https://doi.org/10.3390/ani15050737 - 4 Mar 2025
Viewed by 159
Abstract
The aim of this study is to evaluate the model performance in the classification of FAMACHA© scores using Support Vector Machines (SVMs) with a focus on the estimation of the FAMACHA© scoring system used for early diagnosis and treatment management of parasitic infections. [...] Read more.
The aim of this study is to evaluate the model performance in the classification of FAMACHA© scores using Support Vector Machines (SVMs) with a focus on the estimation of the FAMACHA© scoring system used for early diagnosis and treatment management of parasitic infections. FAMACHA© scores are a color-based visual assessment system used to determine parasite load in animals, and in this study, the accuracy of the model was investigated. The model’s accuracy rate was analyzed in detail with metrics such as sensitivity, specificity, and positive/negative predictive values. The results showed that the model had high sensitivity and specificity rates for class 1 and class 3, while the performance was relatively low for class 2. These findings not only demonstrate that SVM is an effective method for classifying FAMACHA© scores but also highlight the need for improvement for class 2. In particular, the high accuracy rate (97.26%) and high kappa value (0.9588) of the model indicate that SVM is a reliable tool for FAMACHA© score estimation. In conclusion, this study demonstrates the potential of SVM technology in veterinary epidemiology and provides important information for future applications. These results may contribute to efforts to improve scientific approaches for the management of parasitic infections. Full article
(This article belongs to the Section Animal System and Management)
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<p>FAMACHA© card [<a href="#B12-animals-15-00737" class="html-bibr">12</a>].</p>
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<p>Correlation coefficients for each FAMACHA© scores. * <span class="html-italic">p</span> &lt; 0.05, ** <span class="html-italic">p</span> &lt; 0.01, *** <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>Variable importance values.</p>
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<p>Confusion matrix for Support Vector Machine algorithm.</p>
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<p>ROC curve for Support Vector Machine algorithm.</p>
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18 pages, 1553 KiB  
Article
Improvement in the Usability of Meat Inspection Findings for Swine Herd Health Management
by Darko Maric, Sebastian Vetter-Lang, Johannes Klinger, Nikolaus Böhm, Karin Schwaiger and Annemarie Käsbohrer
Animals 2025, 15(5), 688; https://doi.org/10.3390/ani15050688 - 26 Feb 2025
Viewed by 297
Abstract
Data from post-mortem inspections conducted using official controls on the meat production of slaughtered pigs are generally considered valuable for identifying herd health issues and ensuring meat safety. However, several studies highlighted that a multi-stage assessment of lung changes would provide more useful [...] Read more.
Data from post-mortem inspections conducted using official controls on the meat production of slaughtered pigs are generally considered valuable for identifying herd health issues and ensuring meat safety. However, several studies highlighted that a multi-stage assessment of lung changes would provide more useful information on animal health than the implemented binary (yes/no) recording. For this purpose, a new scheme was developed and subsequently used by trained official veterinarians at four slaughterhouses in Austria. Implementation of the multi-stage assessment was carried out in parallel with the conventional assessment, and data collected from both schemes were analyzed and compared to evaluate effectiveness. The analysis of the data (n = 20,345) showed that the most common alteration was low-grade (28.4%), followed by moderate-grade (11.3%,) and then high-grade pneumonia (5.2%). In the case of pleurisy, 88.9% of the carcasses showed no alterations of the pleura, and 11.1% had pathological changes (low-grade pleurisy = 4.7%, moderate-grade pleurisy = 2.7%, high-grade pleurisy = 3.7%). Analysis of the results showed a strong heterogeneity of the frequency of alterations between the batches reflecting various underlying animal health issues. Among the influencing factors, the origin of the pigs had the greatest influence. The project demonstrated that the new evaluation can be carried out easily with no extra time effort once staff are trained and the technological platform for reporting is adapted. The more detailed information ensures more useful feedback is provided to the farmers and supervising veterinarians, thereby ensuing animal welfare and contributing to sustainable, improved animal husbandry. Full article
(This article belongs to the Section Animal System and Management)
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<p>Distribution (proportion of all carcasses) of pneumonia findings according to the new scheme, also broken down by slaughterhouses (numbered SH01 to SH04). The average prevalence for carcasses with no pneumonia (55.1%) is displayed as a dotted line.</p>
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<p>Distribution (proportion of all carcasses) of pleurisy findings according to the new scheme, also broken down by slaughterhouses (numbered SH01 to SH04). The average prevalence for carcasses with no pleurisy (88.9%) is displayed as a dotted line.</p>
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<p>Percentage of alterations for pneumonia and pleurisy with new and old schemes. Only those animals where data for both schemes are available are included (n = 5268).</p>
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<p>Probability of a positive finding in the old scheme for the different groups as a result of categorization according to the new scheme. (<b>A</b>) Data for pneumonia. (<b>B</b>) Data for pleurisy (in brackets: positive carcasses for the old scheme/carcasses in the category according to the new scheme). The <span class="html-italic">p</span>-values shown in the figures are taken from the respective post hoc test of a model explained in <a href="#sec2dot3-animals-15-00688" class="html-sec">Section 2.3</a>.</p>
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<p>Three batches with similar percentages of any type of pneumonia findings (87.7, 88.1, and 86%). The number of animals is given in brackets. The left side is the assessment with the new scheme and the right side is the assessment with the old scheme. A clear difference in severity of findings between the batches compared is visible when the new scheme is applied to the same animals in the slaughter batch.</p>
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<p>Results from farm 1, which delivered animals on 18 different days. A total of 4383 animals were involved, and six batches between 14 November 2022 and 15 December 2022 were evaluated according to both schemes. All findings were collected in SH01. Total 1 shows the average of all 18 batches according to the old scheme. Total 2 shows the average of the 6 batches according to the old scheme that were also evaluated with the new scheme. Total 3 is the average of the 6 batches according to the new scheme.</p>
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<p>Results from farm 2, which delivered animals on 16 different days. A total of 1787 animals were involved, all of which were assessed by the old scheme. Findings were collected in SH04.</p>
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13 pages, 1449 KiB  
Article
Storage Conditions of Sperm Samples and Gametic Characterization by Sperm Head Morphometry in Drones (Apis mellifera)
by Milagros Cristina Esteso, Adolfo Toledano-Díaz, Cristina Castaño, Mariano Higes, Raquel Martín-Hernández, Agustin López-Goya, Pilar De la Rúa, Belén Martínez-Madrid and Julián Santiago-Moreno
Animals 2025, 15(5), 672; https://doi.org/10.3390/ani15050672 - 26 Feb 2025
Viewed by 256
Abstract
The present study aimed to evaluate an optimal method to transport and store drone sperm samples, as well as to characterize drone spermatozoa through sperm head morphometry. A total of 291 mature drones were used. We performed three experiments. In a first experiment, [...] Read more.
The present study aimed to evaluate an optimal method to transport and store drone sperm samples, as well as to characterize drone spermatozoa through sperm head morphometry. A total of 291 mature drones were used. We performed three experiments. In a first experiment, sperm variables were assessed under different incubation conditions (5 °C, 15 °C, and 37 °C with 5% CO2). Results showed that sperm viability was optimally maintained at 15 °C (p ˂ 0.05). In the second experiment, the supplementation of extender with catalase (200 UI) improved (p ˂ 0.05) the sperm viability and motility during liquid storage at different incubation times. Finally, a morphometric analysis of sperm head was made: length 5.13 µm, width 0.85 µm, area 3.78 µm2, perimeter 15.01 µm, acrosome length 3.50 µm. The variability in sperm head morphometry was calculated by coefficients of variation (CV) within- and between-drones. The CV within-drone was higher than the CV between-drones for all morphometric parameters regardless of hive origin, indicating a high degree of sperm pleomorphism. Full article
(This article belongs to the Special Issue Apiculture and Challenges for Future—2nd Edition)
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<p>Sperm motility (%) evaluated at different incubation times in Kiev solution (KIEV) and Kiev solution supplemented with catalase (CATALASE), in drones from FAUNIA. The boxes spread from the 1st to the 3rd quartiles. Box plots show the median (horizontal line), and whiskers extend from the smallest up to the largest value. Different letters (a, b, c) indicate significant differences (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Sperm viability (%) evaluated at different incubation times in Kiev solution (KIEV) and Kiev solution supplemented with catalase (CATALASE), in drones from FAUNIA. The boxes spread from the 1st to the 3rd quartiles. Box plots show the median (horizontal line), and whiskers extend from the smallest up to the largest value. Different letters (a, b, c) indicate significant differences (<span class="html-italic">p</span> &lt; 0.05).</p>
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<p>Sperm motility (%) evaluated at different incubation times in Kiev solution (KIEV) and Kiev solution supplemented with catalase (CATALASE), in drones from CIAPA-IRIAF. The boxes spread from the 1st to the 3rd quartiles. Box plots show the median (horizontal line), and whiskers extend from the smallest up to the largest value.</p>
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<p>Fixed and stained sperm head with Hemacolor<sup>®</sup> for morphometric analysis. (<b>A</b>): Stained sperm head. (<b>B</b>): Measurements: length, width, area, perimeter, and length of acrosome.</p>
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20 pages, 1578 KiB  
Article
Characterization of Goat Production Systems in the Northern Dry Forest of Peru Using a Multivariate Analysis
by Victor Temoche, Irene Acosta, Pablo Gonzales, David Godoy Padilla, Omar Jibája, Juancarlos Cruz and Flor-Anita Corredor
Animals 2025, 15(4), 567; https://doi.org/10.3390/ani15040567 - 16 Feb 2025
Viewed by 328
Abstract
Goat production in the dry forest of northern Peru is essential for rural livelihoods but remains poorly characterized regarding its productivity and sustainability. This study used multivariate techniques—a multiple correspondence analysis (MCA), principal component analysis (PCA), factor analysis of mixed data (FAMD), and [...] Read more.
Goat production in the dry forest of northern Peru is essential for rural livelihoods but remains poorly characterized regarding its productivity and sustainability. This study used multivariate techniques—a multiple correspondence analysis (MCA), principal component analysis (PCA), factor analysis of mixed data (FAMD), and hierarchical cluster analysis (HCA)—to analyze data from 284 producers in Tumbes, Piura, and Lambayeque. Surveys captured 48 variables (41 qualitative, seven quantitative) on productivity, socioeconomics, and management. The MCA explained 22.07% of the variability in two dimensions, while the PCA accounted for 63.9%, focusing on productivity and diversification. The FAMD integrated these variables, explaining 51.12% of variability across five dimensions, emphasizing socioeconomic and management differences. The HCA identified three clusters: cluster 1 featured intensive systems with advanced management and commercial focus, cluster 2 included extensive systems limited by water scarcity, and cluster 3 reflected semi-intensive systems with irrigation and diversified production. These findings provide a detailed understanding of goat systems in northern Peru, identifying opportunities to improve resource use and tailor strategies to enhance sustainability. The multivariate analysis proved effective in capturing the complexity of these systems, supporting productivity and improving livelihoods in rural areas. Full article
(This article belongs to the Section Animal System and Management)
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<p>Map of the study areas in the dry forest of northern Peru.</p>
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<p>Graphical representation of the distribution of modalities for the first (Dim 1) and second (Dim 2) dimensions of the MCA applied to the typology of goat production systems.</p>
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<p>Percentage contribution of each principal component analysis dimension to the total variance.</p>
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<p>A graphical representation illustrates various dimensions’ contributions to the principal component analysis.</p>
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<p>Distribution of goat production system clusters in the dry forest of northern Peru.</p>
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17 pages, 525 KiB  
Commentary
Data Ownership and Privacy in Dairy Farming: Insights from U.S. and Global Perspectives
by Richard Barton, Javier Burchard, Victor E. Cabrera, David Cook, Walter Cooley, Roger Cue, Liliana Fadul, Jay Mattison and Amit Saha
Animals 2025, 15(4), 524; https://doi.org/10.3390/ani15040524 - 12 Feb 2025
Viewed by 743
Abstract
In the evolving landscape of dairy farming, data ownership and privacy have become critical issues. This commentary paper delves into the complexities and implications of data management in the dairy industry, informed by insights from a multidisciplinary group of stakeholders. While the authors [...] Read more.
In the evolving landscape of dairy farming, data ownership and privacy have become critical issues. This commentary paper delves into the complexities and implications of data management in the dairy industry, informed by insights from a multidisciplinary group of stakeholders. While the authors bring a U.S. perspective, the challenges discussed are globally relevant, given the dominant role of multinational corporations in shaping data practices across regions. The discussions, conducted through structured meetings and iterative online exchanges, emphasized the pressing legal and ethical concerns, the role of technology, and the necessity of developing comprehensive guidelines to safeguard data integrity and benefit all stakeholders. Key points include the global nature of data protection regulations, the potential of blockchain and IoT devices to enhance transparency, and the importance of equitable value distribution. By fostering an environment of transparency and fairness, the dairy industry can harness the power of data to drive innovation and sustainability while protecting the rights of those who generate it. This paper provides a pathway to address these challenges by raising awareness and proposing general actions for the industry’s future. Full article
(This article belongs to the Section Animal System and Management)
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<p>Key themes and solutions for addressing data ownership and privacy challenges in dairy farming. The diagram highlights the central focus on data ownership and privacy and their relationship with legal and ethical concerns, intellectual property, technological advancements, global standards, and proposed solutions. The outcomes emphasize transparency, sustainability, the protection of farmers’ rights, and global collaboration.</p>
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14 pages, 2171 KiB  
Article
Individual Cow Recognition Based on Ultra-Wideband and Computer Vision
by Aruna Zhao, Huijuan Wu, Daoerji Fan and Kuo Li
Animals 2025, 15(3), 456; https://doi.org/10.3390/ani15030456 - 6 Feb 2025
Viewed by 406
Abstract
This study’s primary goal is to use computer vision and ultra-wideband (UWB) localisation techniques to automatically mark numerals in cow photos. In order to accomplish this, we created a UWB-based cow localisation system that involves installing tags on cow heads and placing several [...] Read more.
This study’s primary goal is to use computer vision and ultra-wideband (UWB) localisation techniques to automatically mark numerals in cow photos. In order to accomplish this, we created a UWB-based cow localisation system that involves installing tags on cow heads and placing several base stations throughout the farm. The system can determine the distance between each base station and the cow using wireless communication technology, which allows it to determine the cow’s current location coordinates. The study employed a neural network to train and optimise the ranging data gathered in the 1–20 m range in order to solve the issue of significant ranging errors in conventional UWB positioning systems. The experimental data indicates that the UWB positioning system’s unoptimized range error has an absolute mean of 0.18 m and a standard deviation of 0.047. However, when using a neural network-trained model, the ranging error is much decreased, with an absolute mean of 0.038 m and a standard deviation of 0.0079. The average root mean square error (RMSE) of the positioning coordinates is decreased to 0.043 m following the positioning computation utilising the optimised range data, greatly increasing the positioning accuracy. This study used the conventional camera shooting method for image acquisition. Following image acquisition, the system extracts the cow’s coordinate information from the image using a perspective transformation method. This allows for accurate cow identification and number labelling when compared to the location coordinates. According to the trial findings, this plan, which integrates computer vision and UWB positioning technologies, achieves high-precision cow labelling and placement in the optimised system and greatly raises the degree of automation and precise management in the farming process. This technology has many potential applications, particularly in the administration and surveillance of big dairy farms, and it offers a strong technical basis for precision farming. Full article
(This article belongs to the Section Animal System and Management)
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<p>Deployment of experiments.</p>
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<p>Block diagram of UWB positioning system hardware.</p>
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<p>Positioning algorithms.</p>
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<p>BP neural network structure: x is input and y is output.</p>
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<p>Examples of transmission transformations: (<b>a</b>) original image; (<b>b</b>) transformed image.</p>
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<p>Results for the training and test sets: (<b>a</b>) the RMSE of the training set; (<b>b</b>) the RMSE of the test set.</p>
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<p>Process of individual identification: (<b>a</b>) schematic of selected areas; (<b>b</b>) results of yolo testing; (<b>c</b>) coordinate conversion results; (<b>d</b>) target identification results.</p>
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10 pages, 517 KiB  
Article
Safeguarding Bee Health: Insights from a Collaborative Monitoring and Prevention Project Against Pesticide Poisonings
by Mara Gasparini, Giovanni Prestini, Franco Rainini, Gabriella Cancemi, Silvia De Palo, Livio Colombari and Michele Mortarino
Animals 2025, 15(3), 449; https://doi.org/10.3390/ani15030449 - 6 Feb 2025
Viewed by 880
Abstract
In recent decades, bee poisoning due to pesticides and agrochemicals has increased, posing significant challenges to honey bee health and contributing to the so-called “Colony Collapse Disorder” (CCD). Poor knowledge about the level of exposure of bee colonies to pesticides and agrochemicals, whether [...] Read more.
In recent decades, bee poisoning due to pesticides and agrochemicals has increased, posing significant challenges to honey bee health and contributing to the so-called “Colony Collapse Disorder” (CCD). Poor knowledge about the level of exposure of bee colonies to pesticides and agrochemicals, whether from the environment or beekeeping management practices, is a major limiting factor in preventing these diseases. Collaboration among different stakeholders, such as beekeeping associations, local veterinary authorities, and researchers, is essential to create monitoring programs that can collect these data and enable the prompt implementation of surveillance and preventive actions to address potential bee colony poisoning incidents caused by these contaminants. The present study describes the results obtained through a collaborative initiative that was implemented for monitoring and preventing pesticide-induced bee poisonings in a territory of northern Italy where the exposure of honey bee colonies to different types of pesticides and agrochemicals may occur. Four sentinel apiaries were selected based on possible sources of pollution throughout the territory. Pollen samples were collected at different times during the years 2021–2022 and analyzed for pesticides using gas and liquid chromatography tandem quadrupole mass spectrometry (GC-MS/MS and LC-MS/MS) techniques. The findings showed the presence of a number of pesticides, such as pyrethroids, permethrin, cypermethrin, amitraz, and pendimethalin, at varying concentrations. Acute bee mortality was observed in a field case related to pyrethroid exposure in 2022, in an urban area. These results confirm the need for timely interventions, improved sampling methods, and continuous monitoring to safeguard bee populations. Collaboration with local beekeepers and public authorities is thus essential in addressing pesticide use and bee health challenges, fostering efficient communication and training efforts to support sustainable beekeeping. Full article
(This article belongs to the Section Animal System and Management)
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<p>Representation of the study area, delimited in red on the map of the Lombardy region (panel (<b>A</b>)), and the details of the location of the sentinel apiaries S1–S4 (panel (<b>B</b>)). Figure adapted from the original available in [<a href="#B9-animals-15-00449" class="html-bibr">9</a>].</p>
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17 pages, 2331 KiB  
Article
miR-215 Modulates Ubiquitination to Impair Inflammasome Activation and Autophagy During Salmonella Typhimurium Infection in Porcine Intestinal Cells
by Carmen Entrenas-García, José M. Suárez-Cárdenas, Raúl Fernández-Rodríguez, Rocío Bautista, M. Gonzalo Claros, Juan J. Garrido and Sara Zaldívar-López
Animals 2025, 15(3), 431; https://doi.org/10.3390/ani15030431 - 4 Feb 2025
Viewed by 729
Abstract
The host response to S. Typhimurium infection can be post-transcriptionally regulated by miRNAs. In this study, we investigated the role of miR-215 using both in vivo porcine infection models and in vitro intestinal epithelial cell lines. Several miRNAs were found to be dysregulated [...] Read more.
The host response to S. Typhimurium infection can be post-transcriptionally regulated by miRNAs. In this study, we investigated the role of miR-215 using both in vivo porcine infection models and in vitro intestinal epithelial cell lines. Several miRNAs were found to be dysregulated in the porcine ileum during infection with wild-type and SPI2-defective mutant strains of S. Typhimurium, with some changes being SPI2-dependent. Notably, miR-215 was significantly downregulated during infection. To explore its functional role, gain-of-function experiments were performed by transfecting porcine intestinal epithelial cells (IPEC-J2) with a miR-215-5p mimic, followed by label-free quantitative (LFQ) proteomic analysis. This analysis identified 157 proteins, of which 35 were downregulated in response to miR-215 overexpression, suggesting they are potential targets of this miRNA. Among these, E2 small ubiquitin-like modifier (SUMO)-conjugating enzyme UBC9 and E3 ubiquitin-ligase HUWE1 were identified as key targets, both of which are upregulated during S. Typhimurium infection. The miR-215-mediated downregulation of these proteins resulted in a significant decrease in overall ubiquitination, a process crucial for regulating inflammasome activation and autophagy. Consistently, inflammasome markers caspase 1 (CASP1) and apoptosis-associated speck-like protein containing a CARD (ASC), as well as autophagy markers microtubule-associated protein 1A/1B-light chain 3 (LC3B) and Ras-related protein Rab-11 (RAB11A), showed decreased expression in miR-215 mimic-transfected and infected IPEC-J2 cells. To further validate these findings, human intestinal epithelial cells (HT29) were used as a complementary model, providing additional insights into conserved immune pathways and extending the observations made in the porcine system. Overall, our findings demonstrate that miR-215 plays a significant role in modulating host inflammasome activation and autophagy by targeting proteins involved in ubiquitination during S. Typhimurium infection. Full article
(This article belongs to the Special Issue Salmonella and Salmonellosis: Implications in Public Health)
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<p>Fold change protein expression on IPEC-J2 cells in the following three experimental situations (columns) studied: (<b>a</b>) miR-215 mimic-transfected cells compared to mock control cells; (<b>b</b>) cells transfected with the miR-215 mimic and infected with <span class="html-italic">S.</span> Typhimurium compared to mock control cells infected with <span class="html-italic">S.</span> Typhimurium (infection duration 2 h); and (<b>c</b>) mock control cells infected with <span class="html-italic">S.</span> Typhimurium compared to mock control cells (infection duration 2 h). Red indicates overexpression and blue indicates downregulation.</p>
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<p>(<b>A</b>) Ubiquitin expression in IPEC-J2 cells, IPEC-J2 cells transfected with negative controls (mock) and IPEC-J2 cells transfected with miR-215 mimic, 2 h after infection with ST (ratio of the relative quantification intensity to GAPDH below each lane). (<b>B</b>) Relative gene expression (to cyclophilin A) of HUWE1 and UBE2I genes. (<b>C</b>) Ubiquitin expression in HT29 cells, HT29 cells transfected with negative controls (mock) and HT29 cells transfected with miR-215 mimic, 2 h and 24 h after infection with ST (ratio of the relative quantification intensity to GAPDH below each lane). * <span class="html-italic">p</span> &lt; 0.05; **: <span class="html-italic">p</span> &lt; 0.01; ***: <span class="html-italic">p</span> &lt; 0.001.</p>
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<p>(<b>A</b>) Relative gene expression (to cyclophilin A) of inflammasome markers <span class="html-italic">IL1β</span>, <span class="html-italic">CASP1</span> and <span class="html-italic">IL18</span> in IPEC-J2 cells after mock or miR-215 mimic transfection and 2 h infection with <span class="html-italic">S</span>. Typhimurium. (<b>B</b>) Western blot of CASP1, ASC, CASP11 and IL1b in HT-29 cells transfected either with miR-215 or mock controls, 2h and 24h after infection by <span class="html-italic">S</span>. Typhimurium and (<b>C</b>) quantification of Western blot by expression ratio (ratio of relative intensity normalized to GAPDH compared to mock controls). Expression in transfected and infected samples were compared to their infected mock controls for each time point. * <span class="html-italic">p</span> &lt; 0.05; **: <span class="html-italic">p</span> &lt; 0.01.</p>
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<p>(<b>A</b>) Relative gene expression (to cyclophilin A) of autophagy markers <span class="html-italic">MAP1ALC3</span> (LC3), <span class="html-italic">SQSTM1</span> (p62) and <span class="html-italic">LAMP1</span> in IPEC-J2 cells after mock or miR-215 mimic transfection and 2 h infection with <span class="html-italic">S</span>. Typhimurium. (<b>B</b>) Western blot of LC3B and RAB11A in HT-29 cells transfected either with miR-215 or mock controls, 2 h and 24 h after infection by <span class="html-italic">S</span>. Typhimurium. (<b>C</b>) Quantification of Western blot by expression ratio (ratio of relative intensity normalized to GAPDH compared to mock controls). Expression in transfected and infected samples were compared to their infected mock controls for each time point. * <span class="html-italic">p</span> &lt; 0.05; **: <span class="html-italic">p</span> &lt; 0.01; ****: <span class="html-italic">p</span> &lt; 0.0001.</p>
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13 pages, 1373 KiB  
Article
Modelling the Spread of Foot and Mouth Disease in Different Livestock Settings in Italy to Assess the Cost Effectiveness of Potential Control Strategies
by Michele Pesciaroli, Alessandro Bellato, Alessandra Scaburri, Annalisa Santi, Alessandro Mannelli and Silvia Bellini
Animals 2025, 15(3), 386; https://doi.org/10.3390/ani15030386 - 29 Jan 2025
Viewed by 475
Abstract
Foot and Mouth disease (FMD) transmission is strongly related to the distribution and density of FMD-susceptible animals. In this study, we applied a model to simulate the spread of FMD in three Italian regions with different livestock demographics (densely, medium, and sparsely populated) [...] Read more.
Foot and Mouth disease (FMD) transmission is strongly related to the distribution and density of FMD-susceptible animals. In this study, we applied a model to simulate the spread of FMD in three Italian regions with different livestock demographics (densely, medium, and sparsely populated) in order to assess the cost effectiveness of the disease control options within the framework of the Italian contingency plan. The results of the simulations showed that stamping-out was sufficient to control the outbreak in the sparsely and medium populated areas whereas, in densely populated areas, only vaccinations could effectively control an outbreak. We also estimated the economic resources needed for the adoption of different control strategies, considering livestock indemnity, cleaning, disinfection and disposal, surveillance, vaccination and personnel costs. The results obtained showed that indemnity due to culling had the highest impact on the costs in all scenarios and vaccination resulted in the most cost-effective option to apply in densely populated livestock areas. The results of this study can be a useful aid to Competent Authorities in the preparation of contingency plans against FMD. Full article
(This article belongs to the Section Animal System and Management)
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<p>Epidemic curves of the SPLA; median values (solid line) and interquartile range values (shaded areas) of the infected farms per day in the SPLA applying SO.</p>
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<p>Epidemic curves of the MPLA; median values (solid line) and interquartile range values (shaded areas) of the infected farms per day in the MPLA applying SO.</p>
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<p>Epidemic curves for the three control strategies; median values (solid line) and interquartile range values (shaded areas) of the infected farms per day in the DPLA applying SO (upper panel), PC (central panel), and V (lower panel).</p>
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<p>Cost of stamping-out (SO), stamping-out and preventive culling, and (PC), stamping-out and vaccination (V) applied to control the disease in the DPLA.</p>
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12 pages, 2268 KiB  
Article
Milk Performance and Grazing Behaviour of Cinisara Cows Supplemented with Low- and High-Polyphenols Faba Bean Varieties
by Massimiliano Lanza, Marialetizia Ponte, Marianna Pipi, Adriana Bonanno, Antonino Di Grigoli, Marcella Avondo and Serena Tumino
Animals 2025, 15(3), 335; https://doi.org/10.3390/ani15030335 - 24 Jan 2025
Viewed by 510
Abstract
The high degradability of crude protein (CP) from fresh grass can impair its utilization in ruminants. The presence of a moderate level of polyphenolic substances in the diet of grazing animals could help to overcome this problem. The study aimed to evaluate the [...] Read more.
The high degradability of crude protein (CP) from fresh grass can impair its utilization in ruminants. The presence of a moderate level of polyphenolic substances in the diet of grazing animals could help to overcome this problem. The study aimed to evaluate the effects of supplementation with two varieties of faba bean, with different polyphenol contents, on milk yield and quality, as well as on grazing behaviour, in Cinisara grazing cows. A total of 30 cows, homogeneous per days in milk (61 ± 29) and milk yield (12.9 kg ± 3.2), fed on a mixed pasture (CP 17.9% DM) and supplied with grains (an equal mixture of barley, oats, and wheat) and mixed hay (CP 10.2% DM), were assigned to three dietary treatments and supplemented as follows: control group (C, 10 cows), 1 kg of hay, 6 kg of grains mixture, and 0.4 kg soybean meal; low polyphenols group (LP, 10 cows), 1 kg of hay, 4 kg of grains mixture, and 2 kg/d of faba bean var. Torrelama (total polyphenols 4.4 mg GAE/g DM; CP 28.4% DM); high polyphenols group (HP, 10 cows), 2 kg of hay, 4 kg of grains mixture, and 2 kg/d of faba bean var. Fanfare (total polyphenols 16.4 mg GAE/g DM; CP 28.9% DM). All groups were allowed to graze for 20 h/d on natural pasture forage. The HP supplement tended to increase the milk yield compared to that of the LP and C groups (17.1 vs. 15.3 and 14.6 kg/d, respectively; p = 0.057) but reduced the protein (3.20 vs. 3.39 and 3.47%; p = 0.009) and casein proportions (2.45 vs. 2.67 and 2.74%, respectively; p = 0.007) compared to those from the LP and C treatments. HP milk also showed a higher milk urea nitrogen (MUN) value compared to that of C milk, while an intermediate level was measured in LP milk (25.5 vs. 22.9 and 20.9 mg/dl, respectively; p = 0.036). No dietary effect was evident in the milk fatty acid profile. Eating time at pasture and biting rate were not affected by supplementation. The results do not seem to suggest a difference in the efficiency of use of supplements with different polyphenol contents. In any case, they seem to demonstrate that the use of faba beans, regardless of their polyphenol content, represents a valid alternative to soyabeans, also taking into account the tendency for an increased milk yield found by integrating pasture grazing with faba beans possessing a high level of polyphenols, without significant worsening of the qualitative characteristics of the milk or negatively affecting grazing behaviour. Full article
(This article belongs to the Special Issue Sustainable Practices for Forage-Based Livestock Production Systems)
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<p>Audio recording of cow grazing activity. (<b>a</b>) Spectrogram of the recorded bites, showing the signal intensity across frequency and time; distinct bands highlight the spectrum profile of each bite. (<b>b</b>) Waveform of the recording, with peaks corresponding to each biting event.</p>
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17 pages, 1303 KiB  
Commentary
Data Integration and Analytics in the Dairy Industry: Challenges and Pathways Forward
by Victor E. Cabrera, Jeffrey Bewley, Mitch Breunig, Tom Breunig, Walt Cooley, Albert De Vries, Robert Fourdraine, Julio O. Giordano, Yijing Gong, Randall Greenfield, Haowen Hu, Andy Lenkaitis, Mutian Niu, Eduardo A. F. Noronha and Michael Sullivan
Animals 2025, 15(3), 329; https://doi.org/10.3390/ani15030329 - 24 Jan 2025
Viewed by 1259
Abstract
The dairy industry faces significant challenges in data integration and analysis, which are critical for informed decision-making, operational optimization, and sustainability. Data integration—combining data from diverse sources, such as herd management systems, sensors, and diagnostics—remains difficult due to the lack of standardization, infrastructure [...] Read more.
The dairy industry faces significant challenges in data integration and analysis, which are critical for informed decision-making, operational optimization, and sustainability. Data integration—combining data from diverse sources, such as herd management systems, sensors, and diagnostics—remains difficult due to the lack of standardization, infrastructure barriers, and proprietary concerns. This commentary explores these issues based on insights from a multidisciplinary group of stakeholders, including industry experts, researchers, and practitioners. Key challenges discussed include the absence of a national animal identification system in the US, high IT resource costs, reluctance to share data due to competitive disadvantages, and differences in global data handling practices. Proposed pathways forward include developing comprehensive data integration guidelines, enhancing farmer awareness through training programs, and fostering collaboration across industry, academia, and technology providers. Additional recommendations involve improving data exchange standards, addressing interoperability issues, and leveraging advanced technologies, such as artificial intelligence and cloud computing. Emphasis is placed on localized data integration solutions for farm-level benefits and broader research applications to advance sustainability, traceability, and profitability within the dairy supply chain. These outcomes provide a foundation for achieving streamlined data systems, enabling actionable insights, and fostering innovation in the dairy industry. Full article
(This article belongs to the Section Animal System and Management)
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<p>Framework for data integration in dairy farming: from collection to decision support.</p>
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<p>Challenges, proposed solutions, and expected outcomes.</p>
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11 pages, 272 KiB  
Article
Effects of Lipids from Soybean Oil or Ground Soybeans on Energy Efficiency and Methane Production in Steers
by Elizabeth Fonsêca Processi, Tiago Cunha Rocha, Laila Cecília Ramos Bendia, Clóvis Carlos Silveira Filho, Alexandre Berndt, Elon Souza Aniceto and Tadeu Silva de Oliveira
Animals 2025, 15(3), 321; https://doi.org/10.3390/ani15030321 - 23 Jan 2025
Viewed by 454
Abstract
Using lipids can correct energy deficiencies in pasture, boost weight gain in ruminants, and enhance profitability in farming activities. The objective of this study was to evaluate the energy losses in feedlot steers fed diets based on corn silage, with or without the [...] Read more.
Using lipids can correct energy deficiencies in pasture, boost weight gain in ruminants, and enhance profitability in farming activities. The objective of this study was to evaluate the energy losses in feedlot steers fed diets based on corn silage, with or without the addition of lipids in the form of soybean oil or ground soybean seeds. Eight steers were randomly assigned to two balanced 4 × 4 Latin squares. The experimental treatments were as follows: corn silage; corn silage and concentrate feed without lipid addition; corn silage and concentrate feed with 5% lipid (soybean oil) addition; corn silage and concentrate feed with 5% lipid (ground soybean seeds) addition. The results showed that steers fed only corn silage had (p < 0.001) lower dry matter intake (DMI) compared to other treatments. Gross energy intake and gross energy in feces mirrored DMI trends (p < 0.05). Diets with concentrate supplements resulted in higher digestible energy intake (p < 0.05) and increased gross energy in urine. Notably, adding lipids decreased (p < 0.05) methane energy losses, although the processing method did not (p > 0.05) impact these outcomes. In conclusion, adding lipids to the diet reduced energy losses through methane emissions, increasing steers’ energy efficiency. Therefore, the inclusion of lipids reduced enteric methane production in steers. Additionally, the method of lipid processing (soybean oil or ground soybean seeds) did not affect energy partitioning. Full article
21 pages, 6426 KiB  
Article
The Use of Antimicrobials in Animal Husbandry as a Potential Factor for the Increased Incidence of Colorectal Cancer: Food Safety and Kinetics in a Murine Model
by Rosa D’Ambrosio, Stefania Cavallo, Roberta Brunetti, Roberta Pellicanò, Emanuela Vaccaro, Giorgia Borriello, Rubina Paradiso, Francesco Paolo Serpe, Sara Lambiase, Francesca Bruzzese, Giuseppe Palma, Domenica Rea, Antonio Barbieri, Marianna D’Amore, Maria Dimatteo, Barbara degli Uberti, Orlando Paciello and Loredana Baldi
Animals 2025, 15(3), 315; https://doi.org/10.3390/ani15030315 - 23 Jan 2025
Viewed by 614
Abstract
The aim of this research was to investigate the effects of the prolonged use of the broad-spectrum antimicrobial widely used in animal husbandry. By means of a mouse model, a translational study was carried out on immunocompetent mice (with a complete immune system). [...] Read more.
The aim of this research was to investigate the effects of the prolonged use of the broad-spectrum antimicrobial widely used in animal husbandry. By means of a mouse model, a translational study was carried out on immunocompetent mice (with a complete immune system). This study highlighted the effect of antimicrobial residues taken in with food on the growth time of cancer and on alterations to the gut microbiota. This project considered the fight against antimicrobial resistance from a One Health perspectivethrough collaboration between human medicine and veterinary medicine. Regarding food safety, antimicrobial residues in products of animal origin are rarely detected; they therefore constitute a negligible factor in determining colorectal cancer. Full article
(This article belongs to the Section Animal System and Management)
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<p>Time scheme of in vivo experiment.</p>
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<p>The in vitro experiments showed no significant effects of the antimicrobials on the CT26 cell line on viability assays.</p>
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<p>Colony formation assays.</p>
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<p>Trend in tumor growth in females.</p>
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<p>Time-course of tumor growth in males.</p>
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<p>Weight trend in females.</p>
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<p>Weight trend in males.</p>
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<p>Taxa bar plots showing the relative frequencies of the phyla in male control mice (not treated with antimicrobials) (<b>A</b>) and in female control mice (<b>B</b>), at the different experimental times (0, 30, and 60 days).</p>
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<p>Taxa bar plots showing the relative frequencies of the genera identified in male control mice (not treated with antimicrobials) (<b>A</b>) and in female control mice (<b>B</b>), at the different experimental times (0, 30, and 60 days).</p>
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<p>(<b>A</b>) Taxa bar plot showing the relative abundance of the phyla identified in male (<b>A</b>) and female mice (<b>B</b>) treated with sulfonamides at the different experimental times (0, 30, and 60 days).</p>
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<p>Taxa bar plot showing the relative abundance of the genera identified in male (<b>A</b>) and female mice (<b>B</b>) treated with sulfonamides at the different experimental times (0, 30, and 60 days).</p>
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<p>Taxa bar plot showing the relative abundance of the phyla identified in male (<b>A</b>) and female mice (<b>B</b>) treated with tetracycline at the different experimental times (0, 30, and 60 days).</p>
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<p>Taxa bar plot showing the relative abundance of the genera identified in male (<b>A</b>) and female mice (<b>B</b>) treated with tetracycline at the different experimental times (0, 30, and 60 days).</p>
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<p>Immunohistochemical pictures of subcutaneous tumor samples with C4 and CD8 cytoplasmic and perinuclear expression (20x). Control group immunostained CD4 (<b>a</b>) and CD8 (<b>b</b>); group treated with sulfonamides immunostained CD4 (<b>c</b>) and CD8 (<b>d</b>); group treated with tetracyclines immunostained CD4 (<b>e</b>) and CD8 (<b>f</b>).</p>
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18 pages, 1319 KiB  
Article
In a Changing World—An Economical Comparison Between Traditional and Wet-And-Drought-Resistant Grasses in Swedish Cattle Production Under Different Weather Scenarios
by Kristina Holmström, Karl-Ivar Kumm, Hans Andersson, Mikaela Jardstedt, Dannylo Sousa and Anna Hessle
Animals 2025, 15(3), 295; https://doi.org/10.3390/ani15030295 - 21 Jan 2025
Viewed by 597
Abstract
This study compared the profitability when feeding silages of different grass species in enterprises with either dairy cows, beef breed bulls, or beef suckler cows. Traditional (TR) grasses timothy and meadow fescue was compared to the alternative wet-and-drought-resistant (WD) grasses tall fescue, festulolium, [...] Read more.
This study compared the profitability when feeding silages of different grass species in enterprises with either dairy cows, beef breed bulls, or beef suckler cows. Traditional (TR) grasses timothy and meadow fescue was compared to the alternative wet-and-drought-resistant (WD) grasses tall fescue, festulolium, and reed canary grass in three different weather scenarios with either normal conditions (Ref), delayed late harvest time due to wet weather conditions (Wet), or decreased grass yield due to dry weather conditions (Dry). Contribution margin calculation was conducted for three geographical regions in Sweden. In the Ref and Wet scenarios, TR was more competitive than WD for dairy cows and beef bulls in all regions. Also in the Dry scenario, TR was more competitive than WD for dairy cows, as the lower production cost of the WD was outweighed by a lower milk yield of cows fed WD compared to cows fed TR. Contrary, for beef bulls, WD gave a higher contribution margin than TR did in the Dry scenario, where the break-even for WD being superior over TR occurred when more than every second year was dry. WD reed canary grass was always more competitive than TR and WD festulolium for beef cows. Full article
(This article belongs to the Section Animal System and Management)
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<p>Localization of three geographical regions in Sweden; the forest districts in Götaland (Gsk), the plain districts in northern Götaland (Gns), and the lower parts of Norrland (Nn) [<a href="#B25-animals-15-00295" class="html-bibr">25</a>]. Gsk and Gns are situated in the southern part with humid, warm temperate climate and Nn is situated in the north with cool summers.</p>
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<p>Basic calculation of contribution margin (CM = contribution to common cost, risk, and profit) in three different cattle production systems; (<b>a</b>) dairy cow, (<b>b</b>) beef breed bulls, and (<b>c</b>) beef cow, fed silage of traditional (TR) or wet-and-drought-resistant (WD) grasses in forest districts Gsk, plain districts Gns, and northern districts Nn in Sweden, in three scenarios: reference (Ref), wet (Wet), and dry (Dry). TR was timothy (dairy cow), meadow fescue (bull), and meadow fescue-timothy (beef cow), WD was tall fescue (dairy cow and bull), WD-f festulolium (beef cow), and WD-r reed canary grass (beef cow). Expressed as Euro per cow and year or per reared bull.</p>
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<p>Basic calculation of contribution margin (CM = contribution to common cost, risk, and profit) in three different cattle production systems; (<b>a</b>) dairy cow, (<b>b</b>) beef breed bulls, and (<b>c</b>) beef cow, fed silage of traditional (TR) or wet-and-drought-resistant (WD) grasses in forest districts Gsk, plain districts Gns, and northern districts Nn in Sweden, in three scenarios: reference (Ref), wet (Wet), and dry (Dry). TR was timothy (dairy cow), meadow fescue (bull), and meadow fescue-timothy (beef cow), WD was tall fescue (dairy cow and bull), WD-f festulolium (beef cow), and WD-r reed canary grass (beef cow). Expressed as Euro per cow and year or per reared bull.</p>
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