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Antibiotics
Volume 13
Issue 12
10.3390/antibiotics13121224
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This is an early access version, the complete PDF, HTML, and XML versions will be available soon.
Article

High-Risk Lineages of Hybrid Plasmids Carrying Virulence and Carbapenemase Genes

by
Valeria V. Shapovalova
1,*,
Polina S. Chulkova
2,*,
Vladimir A. Ageevets
2,
Varvara Nurmukanova
1,
Irina V. Verentsova
3,
Asya A. Girina
4,
Irina N. Protasova
5,
Victoria S. Bezbido
6,
Victor I. Sergevnin
7,
Irina V. Feldblum
7,
Larisa G. Kudryavtseva
8,
Sergey N. Sharafan
8,
Vladislav V. Semerikov
9,
Marina L. Babushkina
9,
Inna R. Valiullina
10,
Nikita S. Chumarev
11,
Guzel S. Isaeva
11,12,
Natalya A. Belyanina
13,
Irina U. Shirokova
13,
Tatiana M. Mrugova
14,
Elena I. Belkova
15,
Svetlana D. Artemuk
15,
Aleksandra A. Meltser
15,
Marina V. Smirnova
15,
Tatyana N. Akkonen
16,
Nataliya A. Golovshchikova
16,
Oleg V. Goloshchapov
17,
Alexey B. Chukhlovin
17,
Lubov N. Popenko
18,
Elena Y. Zenevich
19,
Aleksandr A. Vlasov
20,
Galina V. Mitroshina
21,
Marina S. Bordacheva
21,
Irina V. Ageevets
2,
Ofeliia S. Sulian
2,
Alisa A. Avdeeva
2,
Vladimir V. Gostev
2,22,
Irina A. Tsvetkova
2,
Maria A. Yakunina
23,
Ekaterina U. Vasileva
23,
Alina D. Matsvay
1,
Dmitry I. Danilov
1,
Yulia A. Savochkina
1,
German A. Shipulin
1 and
Sergey V. Sidorenko
2,22add Show full author list remove Hide full author list
1
Centre for Strategic Planning, of the Federal Medical and Biological Agency, Moscow 119121, Russia
2
Pediatric Research and Clinical Center for Infectious Diseases, Saint Petersburg 197022, Russia
3
Khanty-Mansyisk Regional Clinical Hospital, Khanty-Mansiysk 622018, Russia
4
Khanty-Mansiysk State Medical Academy, Khanty-Mansiysk 628011, Russia
5
Department of Microbiology Named After Associate Professor B.M. Zelmanovich, Krasnoyarsk State Medical University Named After Professor V. F. Voyno-Yasenetsky, Krasnoyarsk 660022, Russia
6
Krasnoyarsk Interdistrict Clinical Emergency Hospital Named After N.S. Karpovich, Krasnoyarsk 660062, Russia
7
Department of Epidemiology and Hygiene, Perm State Medical University Named After Academician E.A. Wagner, Perm 614000, Russia
8
Federal Center for Cardiovascular Surgery Named After S.G. Sukhanov of the Ministry of Health of the Russian Federation, Perm 614013, Russia
9
State Budgetary Healthcare Institution of Perm Krai «Perm Regional Clinical Infectious Diseases Hospital», Perm 614014, Russia
10
Republican Clinical Hospital of the Ministry of Health of the Republic of Tatarstan, Kazan 420064, Russia
11
Department of Microbiology Named After Academician V.M. Aristovsky, Kazan State Medical University of the Ministry of Health of the Russian Federation, Kazan 420012, Russia
12
Kazan Scientific Research Institute of Epidemiology and Microbiology, Kazan 420015, Russia
13
Department of Epidemiology, Microbiology and Evidence-Based Medicine, Federal State Budgetary Educational Institution of Higher Education «Privolzhsky Research Medical University» of the Ministry of Health of the Russian Federation (FSBEI HE PRMU MOH Russia), Nizhny Novgorod 603005, Russia
14
State Budgetary Institution of Healthcare of the City of Moscow «Moscow Science and Practical Centre for the Laboratory Research of the Department of Healthcare of the City of Moscow», Moscow 127015, Russia
15
Mariinsky Hospital, Saint Petersburg 191014, Russia
16
City Polyclinic No. 74, Saint Petersburg 197762, Russia
17
R. M. Gorbacheva Memorial Institute of Oncology, Hematology and Transplantation, Pavlov University, Saint Petersburg 197022, Russia
18
I. I. Dzhanelidze Research Institute of Emergency Medical Service, Saint Petersburg 192242, Russia
19
Alexander Hospital, Saint Petersburg 190068, Russia
20
Psychiatric Hospital No. 1 Named After P. P. Kaschenko, Saint Petersburg 195009, Russia
21
Napalkov State Budgetary Healthcare Institution “Saint-Petersburg Clinical Scientific and Practical Center for Specialised Types of Medical Care (Oncological)”, Saint Petersburg 197101, Russia
22
Department of Medical Microbiology, North-Western State Medical University Named After I.I. Mechnikov, St. Petersburg 191015, Russia
23
Murmansk Regional Clinical-Hospital Named After P.A. Bayandin, Murmasnk 183047, Russia
 
add Show full affiliation list
 
remove Hide full affiliation list
*
Authors to whom correspondence should be addressed.
Antibiotics 2024, 13(12), 1224; https://doi.org/10.3390/antibiotics13121224
Submission received: 7 November 2024 / Revised: 9 December 2024 / Accepted: 16 December 2024 / Published: 17 December 2024
(This article belongs to the Special Issue Antibiotic Resistance and Virulence Mechanisms in Gram-Negative Bacteria: An Alliance for Success)
Review Reports Versions Notes

Abstract

Background/Objectives: Carbapenem-resistant Enterobacterales (CRE) are a global health threat due to their high morbidity and mortality rates and limited treatment options. This study examines the plasmid-mediated transmission of virulence and antibiotic resistance determinants in carbapenem-resistant Klebsiella pneumoniae (Kpn) and Escherichia coli (E. coli) isolated from Russian hospitals. Methods: We performed short- and long-read whole-genome sequencing of 53 clinical isolates (48 Kpn and 5 E. coli) attributed to 15 genetic lineages and collected from 21 hospitals across nine Russian cities between 2016 and 2022. Results: The plasmid analysis identified 18 clusters that showed high concordance with replicon typing, with all clusters having a major replicon type. The majority of plasmids in the IncHI1B(pNDM-MAR)/IncFIB(pNDM-Mar)-like cluster (79.16%) carried both antibiotic resistance genes (e.g., blaNDM-1 and blaOXA-48) and virulence factors (VFs) such as siderophore genes. We hypothesized that hybrid plasmids could play a critical role in the dissemination of antibiotic resistance genes and VFs. Comparative analyses with global plasmid databases revealed high-risk lineages of hybrid plasmids that are predominantly spread throughout Russia at present. Conclusions: Our findings underscore the importance of monitoring plasmid backbones for clinical management, surveillance, and infection control activities.
Keywords: Enterobacterales; carbapenem resistance; virulence; plasmids Enterobacterales; carbapenem resistance; virulence; plasmids

Share and Cite

MDPI and ACS Style

Shapovalova, V.V.; Chulkova, P.S.; Ageevets, V.A.; Nurmukanova, V.; Verentsova, I.V.; Girina, A.A.; Protasova, I.N.; Bezbido, V.S.; Sergevnin, V.I.; Feldblum, I.V.; et al. High-Risk Lineages of Hybrid Plasmids Carrying Virulence and Carbapenemase Genes. Antibiotics 2024, 13, 1224. https://doi.org/10.3390/antibiotics13121224

AMA Style

Shapovalova VV, Chulkova PS, Ageevets VA, Nurmukanova V, Verentsova IV, Girina AA, Protasova IN, Bezbido VS, Sergevnin VI, Feldblum IV, et al. High-Risk Lineages of Hybrid Plasmids Carrying Virulence and Carbapenemase Genes. Antibiotics. 2024; 13(12):1224. https://doi.org/10.3390/antibiotics13121224

Chicago/Turabian Style

Shapovalova, Valeria V., Polina S. Chulkova, Vladimir A. Ageevets, Varvara Nurmukanova, Irina V. Verentsova, Asya A. Girina, Irina N. Protasova, Victoria S. Bezbido, Victor I. Sergevnin, Irina V. Feldblum, and et al. 2024. "High-Risk Lineages of Hybrid Plasmids Carrying Virulence and Carbapenemase Genes" Antibiotics 13, no. 12: 1224. https://doi.org/10.3390/antibiotics13121224

APA Style

Shapovalova, V. V., Chulkova, P. S., Ageevets, V. A., Nurmukanova, V., Verentsova, I. V., Girina, A. A., Protasova, I. N., Bezbido, V. S., Sergevnin, V. I., Feldblum, I. V., Kudryavtseva, L. G., Sharafan, S. N., Semerikov, V. V., Babushkina, M. L., Valiullina, I. R., Chumarev, N. S., Isaeva, G. S., Belyanina, N. A., Shirokova, I. U., ... Sidorenko, S. V. (2024). High-Risk Lineages of Hybrid Plasmids Carrying Virulence and Carbapenemase Genes. Antibiotics, 13(12), 1224. https://doi.org/10.3390/antibiotics13121224

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