Fast Screening of Antibacterial Compounds from Fusaria
<p>The inhibition effects of aurofusarin on <span class="html-italic">Lactobacillus</span> spp. and <span class="html-italic">Bifidobacterium</span> spp. Aurofusarin was tested in concentrations between 2 and 128 µM. The IC<sub>50</sub> is marked with a red dotted line. The values are normalized mean values from three independent experiments. Ethanol 1% is used as control.</p> "> Figure 2
<p>Isolated fractions with antibacterial effect. Secondary metabolites from each fungus were fractionized by preparative HPLC. Fractions in red showed antibacterial effect against <span class="html-italic">Staph. aureus.</span> Fractions in yellow showed antibacterial effect against <span class="html-italic">L. acidophilus</span>. <span class="html-italic">F. avenaceum</span> was grown on YMA, <span class="html-italic">F. graminearum</span> on PDA, <span class="html-italic">F. poae</span> and <span class="html-italic">F. pseudograminearum</span> on YES and <span class="html-italic">F. oxysporum</span> on rice. AUR: Aurofusarin. Enn: Enniatins. ZEA: Zearalenone. RUB: Rubrofusarin. BEA: Beauvericin. Fus C: Fusarin C. BIK: Bikaverin. ?: unknown.</p> ">
Abstract
:1. Introduction
2. Results and Discussion
3. Conclusions
4. Materials and Methods
4.1. Secondary Metabolites
4.2. Bacterial Strains
4.3. Fungal Strains
4.4. Culture Media
4.5. Secondary Metabolite Fractionation
4.6. Antibiotic Susceptibility Tests
4.7. Digital Analysis
4.8. Statistical Analysis
Supplementary Materials
Acknowledgments
Author Contributions
Conflicts of Interest
References
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L. acidophilus | E. coli | Staph. aureus | Salm. typhimurium | |
---|---|---|---|---|
Antibiotic Y | - | - | 32 μM | - |
Beauvericin | 32 μM | - | 32 μM | - |
Enniatin mix | 32 μM | - | 64 μM | 128 μM |
Fusaric acid | 64 μM | 64 μM | - | - |
Aurofusarin | 8 μM | - | - | - |
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Sondergaard, T.E.; Fredborg, M.; Oppenhagen Christensen, A.-M.; Damsgaard, S.K.; Kramer, N.F.; Giese, H.; Sørensen, J.L. Fast Screening of Antibacterial Compounds from Fusaria. Toxins 2016, 8, 355. https://doi.org/10.3390/toxins8120355
Sondergaard TE, Fredborg M, Oppenhagen Christensen A-M, Damsgaard SK, Kramer NF, Giese H, Sørensen JL. Fast Screening of Antibacterial Compounds from Fusaria. Toxins. 2016; 8(12):355. https://doi.org/10.3390/toxins8120355
Chicago/Turabian StyleSondergaard, Teis Esben, Marlene Fredborg, Ann-Maria Oppenhagen Christensen, Sofie K. Damsgaard, Nikoline F. Kramer, Henriette Giese, and Jens Laurids Sørensen. 2016. "Fast Screening of Antibacterial Compounds from Fusaria" Toxins 8, no. 12: 355. https://doi.org/10.3390/toxins8120355
APA StyleSondergaard, T. E., Fredborg, M., Oppenhagen Christensen, A. -M., Damsgaard, S. K., Kramer, N. F., Giese, H., & Sørensen, J. L. (2016). Fast Screening of Antibacterial Compounds from Fusaria. Toxins, 8(12), 355. https://doi.org/10.3390/toxins8120355