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This is a Validated Antibody Database (VAD) review about human PDS5B, based on 3 published articles (read how Labome selects the articles), using PDS5B antibody in all methods. It is aimed to help Labome visitors find the most suited PDS5B antibody. Please note the number of articles fluctuates since newly identified citations are added and citations for discontinued catalog numbers are removed regularly.
PDS5B synonym: APRIN; AS3; CG008; sister chromatid cohesion protein PDS5 homolog B; androgen induced inhibitor of proliferation; androgen-induced proliferation inhibitor; androgen-induced prostate proliferative shutoff-associated protein AS3; androgen-induced shutoff 3

Knockout validation
Bethyl
rabbit polyclonal
  • western blot knockout validation; human; 1:2500; loading ...; fig 6a
  • EMSA; human; 1:50; loading ...; fig 3a
  • immunoprecipitation; human; 1:5000; loading ...; fig 2e
In order to describe a role for APRIN in homologous recombination, Bethyl PDS5B antibody (Bethyl, A300-538A) was used in western blot knockout validation on human samples at 1:2500 (fig 6a), in EMSA on human samples at 1:50 (fig 3a) and in immunoprecipitation on human samples at 1:5000 (fig 2e). Nucleic Acids Res (2016) ncbi
Bethyl
rabbit polyclonal
  • western blot knockout validation; human; 1:2500; loading ...; fig 6a
  • EMSA; human; 1:50; loading ...; fig 3a
  • immunoprecipitation; human; 1:5000; loading ...; fig 2e
In order to describe a role for APRIN in homologous recombination, Bethyl PDS5B antibody (Bethyl, A300-538A) was used in western blot knockout validation on human samples at 1:2500 (fig 6a), in EMSA on human samples at 1:50 (fig 3a) and in immunoprecipitation on human samples at 1:5000 (fig 2e). Nucleic Acids Res (2016) ncbi
rabbit polyclonal
  • western blot; human; fig 4
Bethyl PDS5B antibody (Bethyl Laboratories, A300-537A) was used in western blot on human samples (fig 4). PLoS Genet (2016) ncbi
Abcam
rabbit polyclonal
  • western blot; mouse; fig s2b
In order to elucidate how NEK1 regulates cohesin assembly on chromosomes at meiotic prophase I, Abcam PDS5B antibody (Abcam, ab84918) was used in western blot on mouse samples (fig s2b). Cell Rep (2016) ncbi
Articles Reviewed
  1. Couturier A, Fleury H, Patenaude A, Bentley V, Rodrigue A, Coulombe Y, et al. Roles for APRIN (PDS5B) in homologous recombination and in ovarian cancer prediction. Nucleic Acids Res. 2016;44:10879-10897 pubmed
  2. Brieño Enríquez M, Moak S, Toledo M, Filter J, Gray S, Barbero J, et al. Cohesin Removal along the Chromosome Arms during the First Meiotic Division Depends on a NEK1-PP1γ-WAPL Axis in the Mouse. Cell Rep. 2016;17:977-986 pubmed publisher
  3. Kim J, He X, Orr B, Wutz G, Hill V, Peters J, et al. Intact Cohesion, Anaphase, and Chromosome Segregation in Human Cells Harboring Tumor-Derived Mutations in STAG2. PLoS Genet. 2016;12:e1005865 pubmed publisher