KEGG ENZYME: 1.14.15.44

ENZYME: 1.14.15.44

Entry

EC 1.14.15.44               Enzyme

Name

3-dehydro-2,22-dideoxyecdysone 22-hydroxylase;
CYP302A1;
Disembodied;
dib (gene name)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

3-dehydro-2,22-dideoxyecdysone,reduced adrenodoxin:oxygen oxidoreductase (22-hydroxylating)

Reaction(IUBMB)

3-dehydro-2,22-dideoxyecdysone + O2 + 2 reduced adrenodoxin + 2 H+ = 3-dehydro-2-deoxyecdysone + H2O + 2 oxidized adrenodoxin [RN:R08138]

Reaction(KEGG)

R08138;
(other) R08134

Substrate

3-dehydro-2,22-dideoxyecdysone [CPD:C16498];
O2 [CPD:C00007];
reduced adrenodoxin [CPD:C00662];
H+ [CPD:C00080]

Product

3-dehydro-2-deoxyecdysone [CPD:C16497];
H2O [CPD:C00001];
oxidized adrenodoxin [CPD:C00667]

Comment

This cytochrome P-450 (heme-thiolate) monooxygenase catalyses the second of three sequential hydroxylation reactions that occur during the biosynthesis of 20-hydroxyecdysone, the major molting hormone of arthropods. The enzyme also acts on 3-dehydro-2,22,25-deoxyecdysone and 2,22-dideoxyecdysone.

History

EC 1.14.15.44 created 2024

Pathway

ec00981

Insect hormone biosynthesis

Orthology

K10721

3-dehydro-2,22-dideoxyecdysone 22-hydroxylase

Genes

DME:	Dmel_CG12028(dib)

DER:

6543569

DSE:

6610708

DSI:	Dsimw501_GD13808(Dsim_GD13808)

DYA:	Dyak_GE21407

DAN:

6492970

DSR:

110178636

DPO:

4813322

DPE:

6603297

DMN:

108152978

DWI:

6645984

DGR:

6570685

DMO:	Dmoj_GI13888

DAZ:

108613398

DNV:

108651399

DHE:

111597565

DVI:	6623367(dib)

CCAT:

101460308

BOD:

106616584

BDR:

105224875

RZE:	108371648 108373914

AOQ:

129241681

TDA:

119668349

MDE:

101888518

SCAC:

106084429

LCQ:

111683311

LSQ:

119600747

CVJ:

135953296

GFS:

119645257

ECOE:

129946021

CLON:

129610152

HIS:

119649010

AGA:

1276664

ACOZ:

120954028

AARA:

120905337

AMER:

121592437

ASTE:

118513031

AFUN:

125767141

AMOU:

128305863

AALI:

118463165

AAG:

5574850

AALB:

109399201

ASUA:

134214274

CQU:	CpipJ_CPIJ010826

CPII:

120418865

CNS:

116352451

BCOO:

119080437

LLL:

129794003

PPAP:

129803889

AME:

727118

ALAB:

122717599

ADR:

102676488

AFLR:

100868997

BIM:

100746153

BBIF:

117212506

BVK:

117232412

BVAN:

117165081

BHUN:

126872654

BTER:

100649492

BAFF:

126922409

BPYO:

122574482

BPAS:

132911155

FVI:

122538377

CCAL:

108629141

OBB:

114874541

OLG:

117608485

MGEN:

117226961

NMEA:

116434773

CGIG:

122397116

SOC:

105192796

MPHA:

105833629

AEC:

105151250

ACEP:

105619471

PBAR:

105428630

VEM:

105558182

COBS:

139106541(Dib)

HST:

105188651

DQU:

106751622

CFO:

105255491

FEX:

115239142

PMEC:

138942594(Dib)

LHU:

105671250

PGC:

109857487

OBO:

105276254

PCF:

106792227

PFUC:

122517571

VPS:

122638191

VCRB:

124423880

VVE:

124947356

NVI:

100117435

CSOL:

105363957

TPRE:

106648621

LHT:

122501654

LBD:

127279606

MDL:

103574522

CGLO:

123275338

FAS:

105269303

DAM:

107040638

AGIF:

122848153 122848157

CINS:

118069656

VCAN:

122407444

CCIN:

107272565

DSM:

124416224

NPT:	124215983(dib)

NFB:

124178621

NLO:

107219832

NVG:

124299970

AROA:

105692708

TCA:	663098(CYP302A1)

TMOL:

138130080

DPA:

109546687

ESIM:

136410240(dib)

EFOR:

136339337

SOY:

115876287

AGRG:

126743691

ATD:

109601619

CSET:

123310861

HAXR:

123677405

AGB:

108913130

LDC:	111505902 111516498 111516700

DVV:

114336405

NVL:

108569672

DCOR:

135796074

APLN:

108736612 112905525

PPYR:

116171869

OTU:

111427871

BMOR:

692499(Cyp302a1)

BMAN:

114244688

MSEX:

115442491

DPL:	KGM_215569

BANY:

112054556

MJU:

123866591

NIQ:

126768373

VCD:

124530733

PMAC:

106714854

PPOT:

106105453

PXU:

106125504

BPHI:

137931277

PRAP:

110994687

PBX:

123707503

PNAP:

125055826

ZCE:

119831165

CCRC:

123692788

LSIN:

126977480

AAGE:

121731125

HAW:

110384136

HZE:

124630405

TNL:

113494961

SLIU:

111349746

OFU:

114366503

ONU:

135088626

ATRA:

106131560

GMW:

113511833

PXY:

105389853

PGW:

126366737

LGLY:

125226137

CSTB:

134742902

CFEL:

113376629 113376846

CCRN:

123290451

API:

100165806

DNX:

107162755

AGS:

114132427

RMD:

113555199

ACOO:

126841171

DVT:

126906333

BTAB:

109043630

DCI:

103517403

CLEC:

106669063

HHAL:

106682761

NLU:

111060963

HVI:

124357634

MQU:	128985621 128986476

PHU:	Phum_PHUM246210

FOC:	113205818 113209887

TPAL:

117640519 117647070

ZNE:

110836735

CSEC:

111865689

SGRE:

126284443

BROR:

134529810

IEL:

124160129

HAMR:

139686893

FCD:

110845968

DPX:	DAPPUDRAFT_204789

DMK:

116927720

DPZ:

124327471

AFRA:

136031055

PVM:

113803145

PJA:

122255521

PCHN:

125032298 125042544

PMOO:

119596465

HAME:

121867706

PCLA:

123754302(dib)

CQD:

128686480

PTRU:

123501182

ESN:

127004940

MNZ:

135195035

MRJ:

136831279

PORN:

139748325

HAZT:

108665300 108668730

EAF:

111706681

LSM:

121125186

TCF:

131893440

ISC:

8036952

DSV:

119461020

RSAN:

119396571

RMP:

119174740

VDE:

111252580

VJA:

111265704

TUT:	107360358 112538660

DPTE:

113794166

DFR:

124497872

CSCU:

111637964

CVS:

136974842

PTEP:

107437456

ABRU:

129965503 129981265 129984310 129988671

UDV:	129222269 129224134

SDM:	118189374 118203426 118203441

LPOL:

106466752

CJF:

131872744

» show all

Reference

1 [PMID:16503480]

Authors

Rewitz KF, Rybczynski R, Warren JT, Gilbert LI.

Title

Identification, characterization and developmental expression of Halloween genes encoding P450 enzymes mediating ecdysone biosynthesis in the tobacco hornworm, Manduca sexta.

Journal

Insect Biochem Mol Biol 36:188-99 (2006)
DOI:10.1016/j.ibmb.2005.12.002

Sequence

[msex:115442491]

Reference

2 [PMID:17628274]

Authors

Rewitz KF, O'Connor MB, Gilbert LI

Title

Molecular evolution of the insect Halloween family of cytochrome P450s: phylogeny, gene organization and functional conservation.

Journal

Insect Biochem Mol Biol 37:741-53 (2007)
DOI:10.1016/j.ibmb.2007.02.012