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KEGG   ENZYME: 1.14.15.42
Entry
EC 1.14.15.42               Enzyme                                 
Name
L-tyrosine 3-nitrase;
rufO (gene name);
3-nitro-tyrosine synthase
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
L-tyrosine,nitric oxide, reduced ferredoxin:oxygen oxidoreductase (3-nitro-L-tyrosine-forming)
Reaction(IUBMB)
L-tyrosine + nitric oxide + O2 + reduced ferredoxin + H+ = 3-nitro-L-tyrosine + H2O + oxidized ferredoxin [RN:R13422]
Reaction(KEGG)
R13422
Substrate
L-tyrosine [CPD:C00082];
nitric oxide [CPD:C00533];
O2 [CPD:C00007];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080]
Product
3-nitro-L-tyrosine [CPD:C22981];
H2O [CPD:C00001];
oxidized ferredoxin [CPD:C00139]
Comment
A cytochrome P-450 (heme-thiolate) enzyme characterized from the bacterium Streptomyces atratus. The enzyme participates in biosynthesis of rufomycins, circular heptapeptides with anti-mycobacterial activity. The enzyme requires ferredoxin as its electron donor and catalyses a reaction in which nitric oxide is oxidized to a nitro group and inserted into L-tyrosine at the 3 position (not necessarily in this order).
History
EC 1.14.15.42 created 2024
Orthology
K28017  L-tyrosine 3-nitrase
Genes
AVMJQX13_47220
PAUUE8A73_015495
BMYCDJ92_3552
BARDQRY57_00600
LYQMY533_12020
PEZZHW560_21190
NOZDMB37_11340
SVESVEN_6680
STRCAA958_19375
SCXAS200_45490
SCLFBB341_15470
SGVB1H19_04660
SGXH4W23_34060
SPLACP981_34400 CP981_34405
SATAC5746_05225
SROIIAG44_40665
SXNIAG42_37470
STAALDH80_06615
STUBMMF93_02955
SRPAHEP86_35950 HEP86_35955
SPIQOHA34_26005
ACTQOG417_35255
NOABKM31_20895
SENSACE_6112
SACGFDZ84_23330
AABA4R43_12515
SESPBN6_34140
SSYIEKG83_15705
SACXIOD16_18950
KPHYAOZ06_30045
ACTNL083_5492 L083_5493
ACTLL3i22_059330
PFLAPflav_015550
PRYPrubr_36730
SNASnas_2686
 » show all
Reference
1  [PMID:28774961]
  Authors
Tomita H, Katsuyama Y, Minami H, Ohnishi Y.
  Title
Identification and characterization of a bacterial cytochrome P450 monooxygenase catalyzing the 3-nitration of tyrosine in rufomycin biosynthesis.
  Journal
J Biol Chem 292:15859-15869 (2017)
DOI:10.1074/jbc.M117.791269
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.15.42
IUBMB Enzyme Nomenclature: 1.14.15.42
ExPASy - ENZYME nomenclature database: 1.14.15.42
BRENDA, the Enzyme Database: 1.14.15.42

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