KEGG ENZYME: 1.14.15.29

ENZYME: 1.14.15.29

Entry

EC 1.14.15.29               Enzyme

Name

cholest-4-en-3-one 26-monooxygenase [(25S)-3-oxocholest-4-en-26-oate forming];
CYP125;
CYP125A1;
cholest-4-en-3-one 27-monooxygenase (misleading);
cholest-4-en-3-one,NADH:oxygen oxidoreductase (26-hydroxylating);
cholest-4-en-3-one 26-monooxygenase (ambiguous)

Class

Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor

Sysname

cholest-4-en-3-one,[reduced ferredoxin]:oxygen oxidoreductase [(25S)-3-oxocholest-4-en-26-oate-forming]

Reaction(IUBMB)

cholest-4-en-3-one + 6 reduced ferredoxin [iron-sulfur] cluster + 6 H+ + 3 O2 = (25S)-3-oxocholest-4-en-26-oate + 6 oxidized ferredoxin [iron-sulfur] cluster + 4 H2O (overall reaction) [RN:R11357];
(1a) cholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = (25S)-26-hydroxycholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R09859];
(1b) (25S)-26-hydroxycholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = (25S)-26-oxocholest-4-en-3-one + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H2O [RN:R11355];
(1c) (25S)-26-oxocholest-4-en-3-one + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = (25S)-3-oxocholest-4-en-26-oate + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:R11356]

Reaction(KEGG)

R09859 R11355 R11356 R11357

Substrate

cholest-4-en-3-one [CPD:C00599];
reduced ferredoxin [iron-sulfur] cluster [CPD:C00138];
H+ [CPD:C00080];
O2 [CPD:C00007];
(25S)-26-hydroxycholest-4-en-3-one [CPD:C20143];
(25S)-26-oxocholest-4-en-3-one [CPD:C21304]

Product

(25S)-3-oxocholest-4-en-26-oate [CPD:C21076];
oxidized ferredoxin [iron-sulfur] cluster [CPD:C00139];
H2O [CPD:C00001];
(25S)-26-hydroxycholest-4-en-3-one [CPD:C20143];
(25S)-26-oxocholest-4-en-3-one [CPD:C21304]

Comment

A cytochrome P-450 (heme-thiolate) protein found in several bacterial pathogens. The enzyme is involved in degradation of the host's cholesterol. It catalyses the hydroxylation of the C-26 carbon, followed by oxidation of the alcohol to the carboxylic acid via the aldehyde intermediate, initiating the degradation of the alkyl side-chain of cholesterol [4]. The products are exclusively in the (25S) configuration. The enzyme is part of a two-component system that also includes a ferredoxin reductase (most likely KshB, which also interacts with EC 1.14.15.30, 3-ketosteroid 9alpha-monooxygenase). The enzyme also accepts cholesterol as a substrate. cf. EC 1.14.15.28, cholest-4-en-3-one 27-monooxygenase.

History

EC 1.14.15.29 created 2012 as EC 1.14.13.141, modified 2016, transferred 2018 to EC 1.14.15.29

Pathway

ec00984

Steroid degradation

ec01120

Microbial metabolism in diverse environments

Orthology

K15981

cholest-4-en-3-one 26-monooxygenase

Genes

PHAE:

N1037_04625

SSTL:

ABFK29_23670

MSD:	MYSTI_04828

MLAN:

JY572_02560

PAUU:

E8A73_015680

MTU:	Rv3545c(cyp125)

MTV:	RVBD_3545c

MTC:

MT3649

MRA:	MRA_3584(cyp125)

MTF:	TBFG_13578

MTB:	TBMG_03584

MTK:	TBSG_03611

MTZ:	TBXG_003560

MTI:	MRGA423_22410

MTE:	CCDC5079_3286

MTUR:

CFBS_3761(cyp125)

MTL:	CCDC5180_3238

MTO:	MTCTRI2_3609(cyp125)

MTD:	UDA_3545c(cyp125)

MTN:	ERDMAN_3890(cyp125)

MTJ:	J112_19085

MTUB:

MT7199_3607

MTUC:

J113_24790

MTUE:

J114_18960

MTX:	M943_18225

MTUL:

TBHG_03485

MTUT:

HKBT1_3746(cyp125)

MTUU:

HKBT2_3755(cyp125)

MTQ:	HKBS1_3758(cyp125)

MBO:	BQ2027_MB3575C(cyp125)

MBB:	BCG_3609c(cyp125)

MBT:	JTY_3610(cyp125)

MBM:	BCGMEX_3607c(cyp125)

MBK:	K60_036830

MBX:

BCGT_3411

MAF:	MAF_35570(cyp125)

MMIC:

RN08_3921

MCE:	MCAN_35561(cyp125)

MCQ:	BN44_110037(cyp)

MCV:	BN43_90043(cyp)

MCX:	BN42_90040(cyp)

MCZ:	BN45_100040(cyp)

MORY:

MO_003702(cyp125)

MPA:	MAP_0522 MAP_1614c

MAO:	MAP4_2225 MAP4_3345

MAVI:

RC58_11070 RC58_16590

MAVU:

RE97_11080 RE97_16625

MAV:	MAV_0616 MAV_2811

MIT:	OCO_05260 OCO_26450 OCO_44490

MIA:	OCU_05300 OCU_26300 OCU_44240

MID:	MIP_00975 MIP_04041 MIP_06760

MYO:	OEM_05330 OEM_24920 OEM_44690

MCHI:

AN480_02630 AN480_13920 AN480_23165

MIR:	OCQ_05410 OCQ_25010 OCQ_45630

MMAL:

CKJ54_02555 CKJ54_11950 CKJ54_21190

MLP:

MLM_0760

MMAN:

MMAN_12530 MMAN_32680 MMAN_39010 MMAN_48220

MSER:

MTY59_18030 MTY59_24570 MTY59_42330

MSA:	Mycsm_03408 Mycsm_05668 Mycsm_05807

MUL:	MUL_4106(cyp125A7)

MMI:	MMAR_2783(cyp125A6) MMAR_5032(cyp125A7)

MMAE:

MMARE11_27030(cyp125A6) MMARE11_48420(cyp125A7)

MLI:	MULP_02541(cyp125A6) MULP_05284(cyp125A7)

MPSE:

MPSD_28660 MPSD_52310(cyp125)

MSHO:

MSHO_05050(cyp125) MSHO_41800

MMC:	Mmcs_2723 Mmcs_4572 Mmcs_4677

MKM:	Mkms_2767 Mkms_4660 Mkms_4763

MJL:	Mjls_2753 Mjls_4955 Mjls_5062

MMM:	W7S_02575 W7S_12840 W7S_22405

MKN:	MKAN_00770 MKAN_11895 MKAN_23385

MYV:	G155_05185 G155_16110 G155_26175

MYE:	AB431_16515 AB431_25900 AB431_26415

MHAD:

B586_04585 B586_11505

MDX:	BTO20_16195 BTO20_30765 BTO20_31395

MSHG:

MSG_00803 MSG_02677 MSG_04530

MFJ:	MFLOJ_05510 MFLOJ_32070 MFLOJ_40920

MSTO:

MSTO_06820 MSTO_36070 MSTO_44890

MSIM:

MSIM_21650 MSIM_29340 MSIM_43540 MSIM_50820

MSAK:

MSAS_07800 MSAS_28870 MSAS_51740

MKU:	I2456_00515 I2456_13730 I2456_25550

MLW:	MJO58_04310 MJO58_14090 MJO58_24950

MXE:	MYXE_06050(cyp125) MYXE_06850 MYXE_24960

MNV:	MNVI_11210 MNVI_12270(cyp125) MNVI_41530

MPAG:

C0J29_05145 C0J29_16030 C0J29_27600

MNM:	MNVM_23840(cyp125_1) MNVM_23850(cyp125_2) MNVM_23940(cyp125_3) MNVM_24710 MNVM_32450

MGOR:

H0P51_04720 H0P51_15080 H0P51_25680

MCOO:

MCOO_09740 MCOO_26960 MCOO_45830 MCOO_49100

MBAI:

MB901379_02574 MB901379_04423

MSEO:

MSEO_08790(cyp125) MSEO_14890 MSEO_34240 MSEO_47480

MHEK:

JMUB5695_00503(cyp125) JMUB5695_00597 JMUB5695_02405

MGAU:

MGALJ_01050 MGALJ_16000 MGALJ_37000 MGALJ_38180

MLJ:	MLAC_18500 MLAC_47630(cyp125)

MBRD:

MBRA_20280 MBRA_21690 MBRA_38760

MSHJ:

MSHI_38950(cyp125)

MMAM:

K3U93_12860 K3U93_15220 K3U93_15265 K3U93_22525 K3U93_24190

MSPG:

F6B93_00660 F6B93_11525 F6B93_12530 F6B93_15855 F6B93_20340 F6B93_20510

MOT:	LTS72_10310 LTS72_16725 LTS72_28065

MPAA:

MKK62_02560 MKK62_12590

MKY:	IWGMT90018_09400 IWGMT90018_29370 IWGMT90018_44430 IWGMT90018_57170

MADI:

A7U43_06895 A7U43_07415 A7U43_15580

MWU:	PT015_06050 PT015_15405 PT015_24370

MYW:	BVC93_00785 BVC93_09125 BVC93_26765

MYJ:	BCA37_02880 BCA37_11190 BCA37_15940

MSM:	MSMEG_3524 MSMEG_5853 MSMEG_5995

MSG:	MSMEI_3443(cyp125) MSMEI_5695(cyp125) MSMEI_5834(cyp125)

MSB:	LJ00_17530 LJ00_28945 LJ00_29640

MSN:	LI99_17535 LI99_28950 LI99_29645

MSH:	LI98_17540 LI98_28955 LI98_29650

MVA:	Mvan_3012 Mvan_5151 Mvan_5258

MGI:	Mflv_1508 Mflv_1607 Mflv_3290

MSP:	Mspyr1_08920 Mspyr1_09930 Mspyr1_26180

MCB:	Mycch_2866 Mycch_4512 Mycch_4638

MNE:	D174_15365 D174_23810 D174_24505

MYN:	MyAD_15090 MyAD_23355 MyAD_24040

MFT:	XA26_10010 XA26_33020 XA26_53050(igrA)

MPHL:

MPHLCCUG_00534 MPHLCCUG_00665 MPHLCCUG_02457

MVQ:	MYVA_3054 MYVA_5033 MYVA_5168

MLL:	B1R94_24550

MRH:	MycrhN_0940 MycrhN_2286 MycrhN_2423 MycrhN_4947

MTHN:

4412656_02324 4412656_03978 4412656_04060(cyp125)

MHAS:

MHAS_01888 MHAS_03637 MHAS_03739

MDU:	MDUV_12120 MDUV_49370 MDUV_50510

MCHT:

MCHIJ_19700 MCHIJ_38240

MAUU:

NCTC10437_01632 NCTC10437_02873 NCTC10437_04909 NCTC10437_05013

MMAG:

MMAD_29510 MMAD_47220 MMAD_48270

MMOR:

MMOR_12170 MMOR_13290 MMOR_19000 MMOR_34080 MMOR_47980

MFX:	MFAL_08660 MFAL_23500 MFAL_33000

MAIC:

MAIC_05630 MAIC_06670 MAIC_28630

MIJ:	MINS_00290 MINS_09780 MINS_27860

MALV:

MALV_06950 MALV_42590 MALV_43690

MTY:	MTOK_22340 MTOK_38170 MTOK_47940

MPSC:

MPSYJ_16160 MPSYJ_17100 MPSYJ_35270

MARZ:

MARA_08840 MARA_47760 MARA_49370

MGAD:

MGAD_08800 MGAD_22900 MGAD_36130 MGAD_37260

MHEV:

MHEL_28110 MHEL_28970 MHEL_39560 MHEL_52310

MSAR:

MSAR_09920 MSAR_10700 MSAR_39810

MANY:

MANY_14330 MANY_31860 MANY_32710(cyp125)

MAUB:

MAUB_06600 MAUB_26480

MPOF:

MPOR_34500 MPOR_52540 MPOR_53560

MPHU:

MPHO_06000 MPHO_43880

MMUC:

C1S78_015340 C1S78_025350

MMAT:

MMAGJ_34710 MMAGJ_35720 MMAGJ_65550

MBOK:

MBOE_04320 MBOE_21060 MBOE_41450

MFG:	K6L26_05610 K6L26_06165 K6L26_15795 K6L26_16775

MSEI:

MSEDJ_01010 MSEDJ_02110 MSEDJ_42870

MFLV:

NCTC10271_00498 NCTC10271_00616 NCTC10271_02462

MCEE:

MCEL_07300 MCEL_38100 MCEL_39290

MMON:

EWR22_14470 EWR22_24750 EWR22_25305

MGO:	AFA91_10815 AFA91_11455 AFA91_22195

MHOL:

K3U96_03255 K3U96_03790 K3U96_12335

MSEN:

K3U95_12890 K3U95_13570 K3U95_24665 K3U95_25225

MPAE:

K0O64_15625 K0O64_25340 K0O64_25910

MRF:	MJO55_14510 MJO55_23470 MJO55_23975

MDF:	K0O62_15485 K0O62_25025 K0O62_25750

MCRO:

MI149_16585 MI149_25630 MI149_26220

MGRO:

FZ046_02195 FZ046_02785 FZ046_20385

MAUS:

JN090_14345 JN090_25590 JN090_26370

MFRE:

EXE63_18935 EXE63_19500 EXE63_28710

MKR:	MKOR_35680 MKOR_35690(cyp125) MKOR_37070

MPAK:

MIU77_02130 MIU77_02135 MIU77_02670 MIU77_05555 MIU77_08185

MAB:	MAB_0101 MAB_0611 MAB_0613 MAB_1211c

MMV:	MYCMA_0317 MYCMA_0319 MYCMA_0648 MYCMA_13860

MABB:

MASS_0104 MASS_0581 MASS_0583 MASS_1209

MABL:

MMASJCM_0105 MMASJCM_0600 MMASJCM_0603 MMASJCM_0964 MMASJCM_1238

MCHE:

BB28_00505 BB28_02925 BB28_02935 BB28_06010

MIZ:	BAB75_00800 BAB75_03675 BAB75_03685 BAB75_06600

MSTE:

MSTE_00101 MSTE_00570 MSTE_00572 MSTE_01177

MSAO:

MYCSP_02625 MYCSP_02635 MYCSP_05345 MYCSP_15860

MSAL:

DSM43276_00080 DSM43276_00529 DSM43276_00531 DSM43276_01077

MJD:	JDM601_2803(cyp125A7) JDM601_3609(cyp125A7) JDM601_3682(cyp125A7) JDM601_3692(cyp125A7) JDM601_3693(cyp125A7)

MTER:

4434518_02769(cyp125A7_1) 4434518_03588(cyp125A7_2) 4434518_03654(cyp125A7_3) 4434518_03664(cyp125A7_4) 4434518_03665(cyp125A7_5)

MMIN:

MMIN_05220 MMIN_11390 MMIN_12150

MHIB:

MHIB_22420 MHIB_30110 MHIB_30720(cyp125) MHIB_30810

MHER:

K3U94_16065 K3U94_20385 K3U94_20795 K3U94_20850

MVM:	MJO54_16140 MJO54_20300 MJO54_20710 MJO54_20755

ASD:

AS9A_0965

NFA:	NFA_24320 NFA_25870 NFA_5180

NFR:	ERS450000_01508 ERS450000_03713

NCY:	NOCYR_0531 NOCYR_2255(cyp) NOCYR_4383

NBR:	O3I_002870 O3I_010355 O3I_020195

NNO:	NONO_c05330 NONO_c15420 NONO_c20300 NONO_c31770

NSL:	BOX37_02330 BOX37_04650 BOX37_22005

NSR:	NS506_00434 NS506_01300 NS506_05365

NTP:	CRH09_03475 CRH09_16495

NOZ:	DMB37_00315 DMB37_23770 DMB37_31215

NOD:	FOH10_10445 FOH10_11500 FOH10_28500 FOH10_29905

NAH:	F5544_02805 F5544_08550 F5544_13885

NAD:	NCTC11293_01279 NCTC11293_01796

NWL:	NWFMUON74_05270(cyp125) NWFMUON74_28890 NWFMUON74_42500

NIE:	KV110_03190 KV110_10815 KV110_20625 KV110_22925

NTC:	KHQ06_03465 KHQ06_07690 KHQ06_21070

NHU:	H0264_02870 H0264_04035 H0264_19920 H0264_25955 H0264_32670

NYA:	LTV02_01210 LTV02_14405 LTV02_19940 LTV02_28700 LTV02_29850

NGP:	LTT66_03460 LTT66_15685 LTT66_31145

NSPU:

IFM12276_02110 IFM12276_41710 IFM12276_46530

NYU:	D7D52_03585 D7D52_04685 D7D52_07730

RHA:	RHA1_ro04667 RHA1_ro04679

RER:	RER_09260 RER_15090 RER_33720

REY:	O5Y_04125 O5Y_07155 O5Y_15535

REB:	XU06_04370 XU06_07400 XU06_15770

RQI:	C1M55_04605 C1M55_07640 C1M55_16785

ROP:	ROP_47660 ROP_47780

ROA:	Pd630_LPD01173 Pd630_LPD01185

RPY:	Y013_19380

RBY:	CEJ39_05740 CEJ39_06610

RAV:	AAT18_04130 AAT18_05705

RHW:	BFN03_14725

RRZ:	CS378_19765 CS378_20940

RHQ:	IM25_09115 IM25_09940

RHOD:

AOT96_15010 AOT96_18030 AOT96_26970

RRT:	4535765_00647 4535765_00829

RCR:	NCTC10994_01888 NCTC10994_02073

RTM:	G4H71_07045

RKO:	JWS14_25860 JWS14_25920

RGO:	KYT97_05625 KYT97_11055 KYT97_24330

ROZ:	CBI38_04900 CBI38_04960

RPSK:

JWS13_18580 JWS13_18640

RGOR:

NMQ04_02860 NMQ04_03600

RHOP:

D8W71_26835

RHOO:

RA302_04475 RA302_07545 RA302_15995

RHOU:

KZJ41_04125 KZJ41_06245

RHOW:

A4U64_18845 A4U64_20645

RHOJ:

JVH1_12855 JVH1_12915

RHOS:

QWW67_21465 QWW67_23190

RHOQ:

QWW98_21475 QWW98_23200

RHOY:

QNA09_01675 QNA09_03510

RHOH:

CPI83_13215 CPI83_21635 CPI83_24610

RHOX:

RBB84_24000

REQ:	REQ_07240 REQ_09130 REQ_27290

PDEF:

P9209_16335

PSOW:

ABI214_10020 ABI214_25120 ABI214_25220

WHR:	OG579_07835 OG579_09325 OG579_13015

GBR:	Gbro_3970 Gbro_3974

GPO:	GPOL_c42820 GPOL_c42860

GOR:	KTR9_3902(cyp125A1)

GOQ:	ACH46_17490

GTA:	BCM27_20455

GOC:	CXX93_00535

GIT:	C6V83_03930

GRU:	GCWB2_19940 GCWB2_19965

GOM:	D7316_04508 D7316_04509 D7316_04513

GAV:	C5O27_08150 C5O27_08170

GOD:	GKZ92_18870 GKZ92_18890

GOS:	QAD21_19360

GOJ:	GR168_18705

GAM:	GII34_18850 GII34_18870 GII34_18875

GPD:	GII33_03870 GII33_03890

GJI:	H1R19_18800 H1R19_18820

GOI:	LK459_13780 LK459_13805

GHN:	MVF96_19385

GAMI:

IHQ52_22885 IHQ52_22905

GMG:	NWF22_06365 NWF22_06385

GSI:	P5P27_01195

SPIN:

KV203_10830 KV203_17190

TPR:

Tpau_3821

TSM:	ASU32_21780

TPUL:

TPB0596_41620

TSD:	MTP03_43050

DTM:	BJL86_0735

DIT:	C3V38_06275 C3V38_09410

DIZ:	CT688_03330 CT688_17055

DPC:	A6048_03280 A6048_17675

DLU:	A6035_03570 A6035_17000

DKN:	NHB83_03140 NHB83_17275

DCD:	VYL96_03355 VYL96_16720

TOY:	FO059_13835 FO059_15220 FO059_16580

SMA:	SAVERM_5841(cyp22)

SGR:

SGR_5169

SGB:

WQO_09525

SFI:

SFUL_1917

SANL:

KZO11_09300

SGRF:

SGFS_081120

SCYE:

R2B67_26820

SCB:	SCAB_65311

SVL:	Strvi_7419

SRC:	M271_33995

SMAL:

SMALA_2275

SSOI:

I1A49_14545

SBH:	SBI_07560(cyp37)

SVE:

SVEN_2047

SDV:	BN159_6030(cyp125)

SCI:	B446_12240

STRE:

GZL_06167

SLD:

T261_5756

SLC:	SL103_07525

STRM:

M444_11810

SPRI:

SPRI_5168

SCZ:	ABE83_26245

SCX:	AS200_30925

SRW:	TUE45_02924

SCLF:

BB341_20395

SGS:	AVL59_38775

STSI:

A4E84_12135

SALW:

CP975_10455

SGM:	GCM10017557_58210

SCAD:

DN051_26755

SPHW:

NFX46_32455

SLS:	SLINC_2675

SNR:	SNOUR_28615(cyp125)

SALU:

DC74_2810

SALL:

SAZ_15430

SPLU:

LK06_008595

SAUO:

BV401_14835

SSIA:

A7J05_25220

SVU:	B1H20_08570

SPUN:

BFF78_29375

SGV:	B1H19_13495

SLAU:

SLA_1953

SALF:

SMD44_02573

SALJ:

SMD11_4755

SLX:	SLAV_25570

STRO:

STRMOE7_12495

SFK:

KY5_2401

SNZ:	DC008_09955

SGE:	DWG14_05955

SRJ:

SRO_5110

SLK:	SLUN_11575

SDX:	C4B68_28195

SGD:	ELQ87_27615

SQZ:	FQU76_08425

SCYA:

EJ357_13700

STIR:

DDW44_05615

SKA:	CP970_30575

SGZ:	C0216_24630

SVN:	CP980_23215

SHAW:

CEB94_12585

SRK:	FGW37_09970

SFIC:

EIZ62_23415

SGAL:

CP966_10520

SSPO:

DDQ41_04610

SFY:	GFH48_28195

SAQU:

EJC51_15530

SGF:	HEP81_05415

SCAV:

CVT27_08435

SRIM:

CP984_28545

SSUB:

CP968_23110

SPHV:

F9278_12310

STSU:

B7R87_08360

SCHA:

CP983_30410

SGX:	H4W23_12115

SCOE:

CP976_13630

SSPB:

CP982_13670

SNF:	JYK04_03039

SPLA:

CP981_11780

SBRO:

GQF42_14680

STUI:

GCM10017668_19740

SATA:

C5746_12920

SHUN:

DWB77_05376

SCYG:

S1361_12965

SFEU:

IM697_09955

SDW:	K7C20_11185

SCAL:

I6J39_08770

SAUH:

SU9_009535 SU9_027555

SPEU:

CGZ69_10475

SROI:

IAG44_28495

SXN:	IAG42_24920

SHK:	J2N69_10320

SANU:

K7396_25290

STRY:

EQG64_08555

SGOB:

test1122_05000

SINE:

KI385_14730

SLF:	JEQ17_32325

SDEC:

L3078_13815

SAOV:

G3H79_26265

SDUR:

M4V62_29200

SYAN:

NRK68_10695

SAKB:

K1J60_31795

STAA:

LDH80_27500

SNIG:

HEK616_11590

SCAE:

IHE65_31785

STUB:

MMF93_10175

SSPN:

LXH13_12630

SCIR:

STRCI_002577

SXT:	KPP03845_102563

SLON:

LGI35_15655

STUD:

STRTU_005015

SENG:

OJ254_16455

SGK:	PET44_10430

SANT:

QR300_30255

SYUN:

MOV08_28690

SJN:	RI060_29995

SCYN:

N8I84_12960

SOV:	QZH56_25680

SFIY:

F0344_26615

SHAU:

K9S39_31375

SKG:	KJK29_27050

SCOA:

QU709_30820

SLAI:

P8A22_27445

SFP:	QUY26_28315

STTN:

BSL84_10405

SINN:

ABB07_26645

KBU:	Q4V64_14820

SLUO:

PS467_13325

STPB:

QR97_05450

SRPA:

HEP86_12995

SMIB:

SMIR_27105

SCAJ:

O1G22_29345

STRW:

QHG49_24415

STRL:

HEP84_17820

STSD:

HEP87_18190

SRPZ:

HEP85_12965

SCHE:

QRN89_10355

SCAT:

P8A18_08960

STII:

DRB96_40610

STIC:

DRB89_40340

SABD:

N8I86_12980

SCT:	SCAT_1444(cyp)

SCY:	SCATT_14470

KAB:	B7C62_08510

YIA:	LO772_04365

STRH:

GXP74_08805

AGLA:

OIE69_27530

NCA:

Noca_2739

NDK:	I601_3461 I601_3462

NBE:	Back2_27700

NANO:

G5V58_09915 G5V58_09920

NARO:

CFH99_13390

NPI:	G7071_15770

NPC:	KUV85_04395

NMAR:

HPC71_09655 HPC71_09660

NFC:	KG111_08445 KG111_08455

NBI:	SHK19_12330 SHK19_12335

NOF:	MF408_21210

NOCR:

OSR43_09700 OSR43_09705

PSIM:

KR76_14335

AEB:	C6I20_03435

NGV:	CDO52_23700

TCU:

Tcur_3656

ACTW:

F7P10_32425 F7P10_32430

AGRA:

AGRA3207_006097

AMAZ:

LUW76_38130 LUW76_38135

ACIR:

OG979_07925 OG979_07930

SPIQ:

OHA34_28025 OHA34_31840 OHA34_31845 OHA34_31865

ACTQ:

OG417_15045 OG417_15050 OG417_34220

SRO:	Sros_8218 Sros_8869

NOA:	BKM31_52815

NOW:	GBF35_43300

NCX:	Nocox_35605

NGN:	LCN96_48545

NFS:	OIE67_00480

MHAI:

OHB01_03410

FRI:	FraEuI1c_1505 FraEuI1c_5098

SACG:

FDZ84_22810

AMD:

AMED_2551

AMN:

RAM_12965

AMM:

AMES_2524

AMZ:

B737_2525

AOI:

AORI_2528

AJA:	AJAP_26185(cyp125)

AMQ:	AMETH_4822

AMYC:

CU254_10555

AMYB:

BKN51_05250

AAB:	A4R43_15925

AMYY:

YIM_14520

AORI:

SD37_13695

AACD:

LWP59_29050

APRT:

MUY14_23655

AROO:

NQK81_09710

ATHM:

L1857_26850

ARHD:

VSH64_02045

AMOG:

QRX60_26510

ACYN:

ORV05_07375

ACOR:

LCL61_01450

AMYZ:

HUW46_00300

ACAB:

QRX50_44235

ANW:	QP939_49650

AMYD:

K1T34_30925 K1T34_52240

PSDS:

K1T35_06325

KAL:

KALB_3393

KUT:	JJ691_41230

KPHY:

AOZ06_42520

LED:	BBK82_38815

ALO:

CRK59579

PMAD:

BAY61_10985

SACE:

GIY23_13310

CROS:

N8J89_13150

STP:	Strop_2612

SAQ:

Sare_2811

AMS:	AMIS_19330

AIH:	Aiant_18690

ACTL:

L3i22_084210

ASIC:

Q0Z83_065430

AOU:	ACTOB_004137

ACTE:

ACTI_41350

DACT:

KZZ52_43540

CAI:

Caci_0773

JCY:	M6B22_21200

ABAI:

IMCC26256_11916

AQZ:	KSP35_04805 KSP35_04815 KSP35_14595

IAM:	HC251_10060 HC251_15180 HC251_17255 HC251_23270

IMA:	PO878_12445 PO878_16050 PO878_16685 PO878_20710

DHI:	LH044_16665

» show all

Reference

1 [PMID:19843222]

Authors

Rosloniec KZ, Wilbrink MH, Capyk JK, Mohn WW, Ostendorf M, van der Geize R, Dijkhuizen L, Eltis LD

Title

Cytochrome P450 125 (CYP125) catalyses C26-hydroxylation to initiate sterol side-chain degradation in Rhodococcus jostii RHA1.

Journal

Mol Microbiol 74:1031-43 (2009)
DOI:10.1111/j.1365-2958.2009.06915.x

Sequence

[ag:ACU30137]

Reference

2 [PMID:19846552]

Authors

McLean KJ, Lafite P, Levy C, Cheesman MR, Mast N, Pikuleva IA, Leys D, Munro AW

Title

The Structure of Mycobacterium tuberculosis CYP125: molecular basis for cholesterol binding in a P450 needed for host infection.

Journal

J Biol Chem 284:35524-33 (2009)
DOI:10.1074/jbc.M109.032706

Sequence

[mtu:Rv3545c]

Reference

3 [PMID:19846551]

Authors

Capyk JK, Kalscheuer R, Stewart GR, Liu J, Kwon H, Zhao R, Okamoto S, Jacobs WR Jr, Eltis LD, Mohn WW

Title

Mycobacterial cytochrome p450 125 (cyp125) catalyzes the terminal hydroxylation of c27 steroids.

Journal

J Biol Chem 284:35534-42 (2009)
DOI:10.1074/jbc.M109.072132

Sequence

[mtu:Rv3545c]

Reference

4 [PMID:20545858]

Authors

Ouellet H, Guan S, Johnston JB, Chow ED, Kells PM, Burlingame AL, Cox JS, Podust LM, de Montellano PR

Title

Mycobacterium tuberculosis CYP125A1, a steroid C27 monooxygenase that detoxifies intracellularly generated cholest-4-en-3-one.

Journal

Mol Microbiol 77:730-42 (2010)
DOI:10.1111/j.1365-2958.2010.07243.x

Sequence

[mtu:Rv3545c]

Other DBs

ExplorEnz - The Enzyme Database:

1.14.15.29

IUBMB Enzyme Nomenclature:

1.14.15.29

ExPASy - ENZYME nomenclature database:

1.14.15.29

BRENDA, the Enzyme Database:

1.14.15.29

DBGET integrated database retrieval system