#=GF ID   CNF1
#=GF AC   PF05785.17
#=GF DE   Rho-activating domain of cytotoxic necrotizing factor
#=GF AU   Moxon SJ;0000-0003-4644-1816
#=GF SE   Pfam-B_7489 (release 8.0)
#=GF GA   25.00 25.00;
#=GF TC   25.00 25.80;
#=GF NC   24.00 19.50;
#=GF BM   hmmbuild HMM.ann SEED.ann
#=GF SM   hmmsearch -E 1000 --cpu 4 -Z 81514348 HMM pfamseq
#=GF TP   Domain
#=GF WK   Cytotoxic_necrotising_factor_family
#=GF CL   CL0663
#=GF RN   [1]
#=GF RM   12622819
#=GF RT   Expression of cnf1 by Escherichia coli J96 involves a large
#=GF RT   upstream DNA region including the hlyCABD operon, and is
#=GF RT   regulated by the RfaH protein. 
#=GF RA   Landraud L, Gibert M, Popoff MR, Boquet P, Gauthier M; 
#=GF RL   Mol Microbiol 2003;47:1653-1667.
#=GF RN   [2]
#=GF RM   12065482
#=GF RT   Identification of a receptor-binding domain of Bordetella
#=GF RT   dermonecrotic toxin. 
#=GF RA   Matsuzawa T, Kashimoto T, Katahira J, Horiguchi Y; 
#=GF RL   Infect Immun 2002;70:3427-3432.
#=GF RN   [3]
#=GF RM   11427886
#=GF RT   Structure of the Rho-activating domain of Escherichia coli
#=GF RT   cytotoxic necrotizing factor 1. 
#=GF RA   Buetow L, Flatau G, Chiu K, Boquet P, Ghosh P; 
#=GF RL   Nat Struct Biol 2001;8:584-588.
#=GF DR   INTERPRO; IPR008430;
#=GF DR   SCOP; 1hzg; fa;
#=GF DR   SO; 0000417; polypeptide_domain;
#=GF CC   This family consists of several bacterial cytotoxic necrotizing
#=GF CC   factor proteins as well as related dermonecrotic toxin (DNT)
#=GF CC   from Bordetella species. Cytotoxic necrotizing factor 1 (CNF1)
#=GF CC   causes necrosis of rabbit skin and re-organisation of the actin
#=GF CC   cytoskeleton in cultured cells [1]. Bordetella dermonecrotic
#=GF CC   toxin (DNT) stimulates the assembly of actin stress fibres and
#=GF CC   focal adhesions by deamidating or polyaminating Gln63 of the
#=GF CC   small GTPase Rho. DNT is an A-B toxin which is composed of an
#=GF CC   N-terminal receptor-binding (B) domain and a C-terminal
#=GF CC   enzymatically active (A) domain [2].
#=GF SQ   23
#=GS Q2KUX5_BORA1/1173-1456      AC Q2KUX5.1
#=GS A0A160FJK5_9BURK/2078-2183  AC A0A160FJK5.1
#=GS A0A6J5E7H3_9BURK/161-428    AC A0A6J5E7H3.1
#=GS A0A160FPS5_9BURK/1889-2166  AC A0A160FPS5.1
#=GS A0A494XEK9_9BURK/2517-2736  AC A0A494XEK9.1
#=GS A0A1I5A037_9GAMM/107-375    AC A0A1I5A037.1
#=GS A0A6J5DN08_9BURK/187-430    AC A0A6J5DN08.1
#=GS D0Z517_PHODD/723-1009       AC D0Z517.1
#=GS A0A085U2F6_YERRU/99-367     AC A0A085U2F6.1
#=GS A0A077PC09_XENBV/1-156      AC A0A077PC09.1
#=GS Q7VTS2_BORPE/1170-1454      AC Q7VTS2.1
#=GS A0A6J5DUT7_9BURK/305-512    AC A0A6J5DUT7.1
#=GS A0A6J5EC43_9BURK/426-651    AC A0A6J5EC43.1
#=GS D4EA17_SEROD/1-138          AC D4EA17.1
#=GS A0A160FJK5_9BURK/1871-2075  AC A0A160FJK5.1
#=GS Q87GJ5_VIBPA/88-360         AC Q87GJ5.1
#=GS A0A0J6NMF7_9NEIS/123-394    AC A0A0J6NMF7.1
#=GS A0A160FPA1_9BURK/2352-2613  AC A0A160FPA1.1
#=GS A0A1N6KFU4_9BURK/280-564    AC A0A1N6KFU4.1
#=GS A0A014NAG0_9GAMM/694-969    AC A0A014NAG0.1
#=GS A0A6J5DYF9_9BURK/422-653    AC A0A6J5DYF9.1
#=GS A0A160FPA1_9BURK/1304-1539  AC A0A160FPA1.1
#=GS U3ALL7_9VIBR/18-287         AC U3ALL7.1
Q2KUX5_BORA1/1173-1456                 ..........................................................................TLANTD...TGRLNHGDISVLRGRGAIGGK..RTVA.LPSALLIDYVRIDS..D.LL.MDRAERLTNEAFMRQGGVLHGFVQSW..LGSEI.TSTT.TG.....G.MRLGK.WGPGQQ...LSSNVRVLRVSNGNEGTIGLRI--SALPTGMPLILTAGELSGCTSIVAS..KD..GALYFYHFGRR.EGKG...GWLTSREG.VAT...VYRSHLAFG...L.PSV.P.NLEPN..........SY.D.DLVDMLAV.YDRAAVVYLGKEREGGGG...RISR.........SLPANVHVLDYNAAT..A.AADSARLGLTFALVS.SD.GTG.VKWSLLAEDDAIALASAELNVLAS..........................................................
A0A160FJK5_9BURK/2078-2183             ...............................................................delldnagtsl------...---------------------..----.--------------..-.--.--------------------------..-----.----.--.....-.-----.------...-------------------------------------------------..--..-----------.----...--------.---...---------...-.---.-.----R..........GN.D.VLVDMIST.YHKGTINYFGKYIPGEVD...AMVT.........NAKANVNSFDYNKIK..S.DPYGSRVGIAYALLV.KK.SGR.VKVATYSEDMSILMKNGEIN----ynvld.....................................................
A0A6J5E7H3_9BURK/161-428               ......lnlepatgpaadiarirqfgmaltfyrndksasqtrdlssidktayearhdviafgkgtdgtsyvpea------...---------------------..----.--------------..-.--.--------------------------..-----.----.--.....K.AYIGR.AWTTTP..tELSGTDIVKLGAGKDGTAAIMLPFDQLRLGSTVIVTGGPMRGSTMLFAA..DN..HGFYAYHAGTS.SNEP...RWTVSEDG.ARS...IANAYRAMH...P.AHH.AwITARS..........GA.D.ELVSIAKL.EPFSALIYNGAYSTEPGR...KLP-.........---------------..-.---------------.--.---.------------------------derihaplhatgtepndpwhmmtfsyfepgdvrsvgtaqaviskdaggkitvqvmgek
A0A160FPS5_9BURK/1889-2166             .........................................................................q-LTHTD...IGDLVQGDINPLARNGLLYRK..SSGE.IRLGFDA--TRFDS..HtIE.MKKEFSLADSKFSD-------VLDTW..KGGDT.TSQG.RD.....D.VWSGT.WGKDNL...LNDDISIVELTSGKSGTAAIRIKLEDVKEGYPVVVSGGELSGSTIVFGV..DE..QYFYAFHAGQK.TDDQ...KWRPDQEG.AAS...IYQAYLALNgevI.PSL.G.ISEDMalvnehnelaGN.R.GIVDILEK.YESGFFAFSGKSAGSPPG...--EM.........NAEKPIEWLDYAPA-..A.TDSAVEKGRAYALLA.RC.DGD.LKITAYAE----------------gassmh....................................................
A0A494XEK9_9BURK/2517-2736             ................................................rmqagdrninfdefidfssgtqmltr------...---------------------..----.--------------..-.--.--------------------------..-----.----.--.....-.-----.-KGKQA...VPDNVVTITVANGDSGVVATKIPLNAVGKDTPILAYSGDLSGCTVVYAV..RG..DHFYVYHAGLRsNAEGldaNWRTGREG.VHA...LIESH-KVL...T.GEL.V.GELAL..........HN.D.SLVDLFST.YDQAVITYLGKEGTKIGK...----.........-AAPNVGVFDYAEAP..G.KKFSARVGESHAILA.RS.NDE.VKIEVLSEDYLSEV----------kvgrsrddvind..............................................
A0A1I5A037_9GAMM/107-375               .....................................................................kesas------...---VMNGNIRPFIGKGIISME.aKNDN.LPV--TYDYIISDK..N.--.---HGILRENISSVNFG-----IECY..NGDKI.KSAEiIP.....G.TPIGN.YYNKQT...FNHDLKIIQLDSGNCGTVGFRLDINQLTPDRPLLIHGGELSGCTMVYGL..KD..NYFYAFHSGQPgNDKS...IWETKKDG.SKS...IIESHNKMT...G.STS.D.TDKEI..........DI.E.TLSSYLHDnFDICMMAFCGHGETIGSK...----.........---KKVLSFDYNKPSilV.GENKSRVANTMAMLV.NK.GGV.IEINMLSDDMTIDKKTLETDSM--ty........................................................
A0A6J5DN08_9BURK/187-430               ...............fyrndrsesgtrnpslidktryrarhdvvavgkgtdatsyvpeakaymgrawsttptel------...---------------------..----.--------------..-.--.--------------------------..-----.----.--.....-.-----.------...--SGTDVVKLGAGKDGTAAIMLPFREIRPGSTIIVTGGPMRGSTMLFAA..DH..HGFHAYHAGSS.SDHP...RWSVSEDG.ARS...IANAYRTMH...P.GHD.AwITARS..........GA.E.ELVSIARQ.YPFAALIYNGEYSTEPGR...KLP-.........---------------..-.---------------.--.---.------------------------derihaplhasgtdphdpwhmmtfsyfepgdvrsvgtaeaviskdargkvtvqvlgek
D0Z517_PHODD/723-1009                  ...................................................................aenasin------...FQKLAKGDINVLKGRGSISST..RQRA.IEPYFIS----SNA..D.-E.LQGGFYTKKDRFNNLGYDTHFYNSSIgsNGAPT.LNTY.TGeiltdS.SSLGStYWKEHN...LTNNTNIIRVSNSARGANGIKIELEQVQANKPVIITSGNLSGCTTIVAR..KG..GWLYKVHTGTT.EPVA...GF-TSTTG.VQKaveVFELLLKDG...A.GFP.K.VKGIM..........NN.D.FLVNYLADnFDESLITYASSDAKQGSI...-ITV.........-SRDNVLTFPYYTN-..-.-DNLLGFGTSATILArVD.NNT.L-VSSLSESYSLTTDG--------akvsisk...................................................
A0A085U2F6_YERRU/99-367                .......................................................................lin-----D...KRAISNGNLAPLVGQGIISSN.vRNSD.MPI--SINYVCLDK..N.--.---TQKVMLDTKSTDFG-----MNFF..RGDDA.QSAElIP.....G.LPFGN.YYNQQK...FDNNLHVLTVNNGERGTIGCQINLAKIEEGYPLLINGGKLSGCTMVYAV..KG..NSLFIYHAGKSgNDAS...DWKTGSDG.VRS...IIDAHSALT..sL.KND.P.LDSRA..........DN.Q.GLVNYLEShFDFYSLTYCGHGEAVEGG...----.........---EN--VFDYNQDR..AtTDDNVRVGNAMALIV.KH.NGI.IKIQTMSDDMTIDKKNVETQSVA-h.........................................................
A0A077PC09_XENBV/1-156                 .......................................................................mra------...---------------------..----.--------------..-.--.--------------------------..-----.----.--.....-.-----.------...--------------------------IEEKKPIVITSGGLSGCTMLCAH..DQ..QYFYAYHTGQS.N-PS...EWRTGVGG.VKT...TAESHR-LL...T.GKD.P.GEPEP..........HN.N.SLITLFEN.YESAHITYFGK----VGT...KITE.........-NSEKVLTSDYNSF-..-.KSFDPAIGYAYALLA.RV.GNK.VKIKTLSEETHISKSG--------klnfkss...................................................
Q7VTS2_BORPE/1170-1454                 ..........................................................................DYANTD...IGLLNHGDISPLRARPPLGGR..RDID.LPPGLDISFVRYDR..P.VR.MSAPRALDASVFRPVDGPVHGYIQSW..TGAEI.EYAY.--.....G.APAA-.-AREVM...LTDNVRIISIENGDEGAIGVRVRLDTVPVATPLILTGGSLSGCTTMVGV..KE..GYLAFYHTGKS.TELG...DWATAREG.VQA...LYQAHLAMG...Y.API.S.IPAPM..........RN.D.DLVSIAAT.YDRAVIAYLGKDVPGGGS..tRITRh.......dEGAGSVVSFDYNAAV..Q.ASAVPRLGQVYVLIS.ND.GQG.ARAVLLAEDLAWAGSGSALDVLNE..........................................................
A0A6J5DUT7_9BURK/305-512               ......................................................ydaeekagvngdkaadmtpe------...---------------------..----.--------------..-.--.--------------------------..-----.----.--.....-.-----.------...-LANASVIQLGSGKDGVAAVNVCFEDLAPGSTTIVSSGPMRGSTMLFTA..DE..TGFSAYHAGAS.KQTR...AKAADAEA.-SS...IAEAHERFG...S.THAdA.TPQQN..........GM.D.ALYRAATShPFSAIVSSYGDPPAGQPH..pKYAPsndaglgadGRPWSMMNFNYVGA-..-.DQSPREVGSAEAIIR.KDeNGK.ITVQVLA-----------------ergklgy...................................................
A0A6J5EC43_9BURK/426-651               .....................................vgdlkriagkgpisialantplddsirvrdlgsqfff------...---------------------..----.---------YRKDR..D.ITdMNEPRAVRGTDYEHRRDVLALGNGEQ..ATSYV.ANSK.IA.....A.GTFWG.YGPATH...LQG-AQLIELGNGRDGVGAIKLPFASMRPGSTVIVSGGAMNGCTMLFAS..DG..KALYAYHAGST.EMSP...NWLTSREG.AQS...IVDAHTKIG..pK.DQV.P.YRWHG..........TN.D.DLVTIGRQ.YPFSALIYSGQ-------...----.........---------------..-.---------------.--.---.------------------------ylanthavvgagn.............................................
D4EA17_SEROD/1-138                     .....................................................................mhehg------...---------------------..----.--------------..-.--.----------------------VYSY..NGLSI.ESAEiIP.....G.TPMGN.YHNKQM...YPEGLNVIEIANGNCGVIGIRFHLGQLKSNNPLLIHGGALSGCTIAFAI..KD..DCFYAFHCGQSgNNKY...LWETSREG.VDS...IINAHHKLI...G.THS.K.EKVKP..........GL.Q.VLV-----.------------------...----.........---------------..-.---------------.--.---.------------------------er........................................................
A0A160FJK5_9BURK/1871-2075             ...................................................................vdagknv------...--ELKGGDISPLAGKGVLFGK..ITR-.-TPDVEFTFVRNDAvtD.TT.ATPARKFSMAEVTDENGALHGVIETW..SGEQV.TSKA.RN.....G.PALGL.WGDKFS...LDADFSVMEVSNGKSGTVGISIPFDQLREGKPIVISSGELTGCSMIYAV..DD..NKFYAYHAGQS.PGDS...KWLTGKHG.AAS...IYKAHLSLT...G.KSL.P.D----..........--.-.--------.------------------...----.........---------------..-.---------------.--.---.------------------------vqiapgnirdkngmvrl.........................................
Q87GJ5_VIBPA/88-360                    ..........................................................................NVKKADaksITALKSGDLHILKGKGIIGMK.gGDNK.LPFKCTI--VNDDK..N.--.---GAHLSQGTNLATNG-----IKSM..AGDSV.RAAQlIP.....G.TPLGQ.FYNSAP...LDDSFNVVHLPNGQRGVNGLKIPLSEFYSEKKFLFSNGALSGCMTCTAI..DK..NNLYIFHVGKDgNDTS...PWKTNVDG.-SS...LIQKNMKML...L.GQN.S.DSLNN..........GI.Q.GLIDYCSKnFDKAIIQYCGHGEQYSGR...----.........---KNIHLFDYNTP-..Q.KNNPLRVGNNLTLIShSD.NGS.LSISTLCDDMIINSKTCETNSVNS..........................................................
A0A0J6NMF7_9NEIS/123-394               ......................................................................lfer-----D...KAQVLSGSVAPLIGKGVLASA.aGVQA.AAPAGVVDCIRYDR..D.--.---GEKKG------GDAVSADFIKFY..HGDQA.KAEElIP.....G.TPIGN.HYNKFQ...FGDALRLIEIGNGLQGTVGMKFDTGKMQDGDCCLFSAGALSGCTMVYAL..KG..GMLYAYHAGTVkNGDD...EWETASDG.ARS...IVDAHRQLS...G.EKT.A.LDSPV..........GN.NrELAAFLHEhFDHAMMVYSGKGTTPDLA...----.........-DSGHVRHFDYNKTP..L.RQELASVGNALALLK.KT.GDS.LSIEFLGDSMRINHN---------cgvhsqd...................................................
A0A160FPA1_9BURK/2352-2613             ........................................................................te---NAE...LKKLLSGNIAAAIGKGAIYPApnRAVR.-SFPWNFVFARSDS..Q.IT.HDSPQPSTE---LGTTSPY--DIQLG..QGSDIvSYSQ.SS.....R.VSYGD.FWSGEN..dELQKLDVILVPNGNDGANGIKISLDELIPGRPLLISSGALSGCTQIICI..KKseNSIYIYHAGKH.SPDT...KWQTQADG.VKH...IAHVHGVLT...G.QKS.S.LVLPDe........pTS.A.DLTGLYGY.FDNTYIMSSRPLESTSPT...RIER.........QD-------------..-.---------------.--.---.------------------------sthwaysykgdegrfsvgnsqllilkdskgdvq.........................
A0A1N6KFU4_9BURK/280-564               .......................................................................kfs----SD...AHRIRNGKIEALKNKGAIYVG..EKSE.IR--TNFDFYRYDN..D.VK.MSEPVKSSNAGTEKTREVL----YTG..NGKGI.TSLC.QG.....GkMYYGG.WGRGNR..kLSSQTDVIELANGKEGVGAVRIAFADIPPKGSLLVTAGGLSGCTVMFAA..DN..AHFYAYHVGTS.TPTR...NWKTAQDG.VRL...LRQAHEQLK...P.DAT.RkALTHP..........EN.N.DLIDVAKQ.YPFASVIYYGKHGESGAQadtRITGa......agPAAQGTHIHDYFQP-..-.DVKGAPIGTAFALIR.KD.ARGrISVSTLYE----------------rgelpianyksdak............................................
A0A014NAG0_9GAMM/694-969               ......................................................................lfvs-----D...DQQLKKGSITPLRSRGLLVGS..DTVE.TSLQFDI--HRYDS..D.KH.LSEPLPYTSEI-LNEESILHKTVMVF..DGQHM.TSSE.SG.....A.GKIGS.HWDSNK...LSKDIQGIQVANGMSGSVGIRIALDSIHDGNPLIVTSGALSGCSMIYTV..DD..DHFYALHTGQK.PGDN...KWKTGLHG.VAS...-AQHTLQAL...S.GKT.L.DTSGN..........HN.N.DLIDTLGL.FDNSALTFFGKEGTRTDR...----.........-VKSNVTTFDYNLAQ..A.PKFSIRAGYSYALLA.KK.QGK.TIVKVLSEDVSVNSMNKKINVLG-s.........................................................
A0A6J5DYF9_9BURK/422-653               ....................................aggdlkriagkgpinvmpaatplddgkritnfgapfff------...---------------------..----.---------YRKDR..D.VTdMDGPRAVRGTDYDHRREVLVVGDGTQ..TTSQI.ARSK.--.....-.ISAGT.FWGSGPashL-QGAEVIKLGNGRDGVGAIKLPFASIKPGEAVVVSGGAMNGCTMLFAS..DG..KSLYAYHAGLS.QLTP...NWRTAREG.AQA...IVDAHVVMG...PkEQI.R.YKWYG..........SN.D.DLITIGRQ.YPFSALIYNSRDLSGT--...----.........---------------..-.---------------.--.---.------------------------nalvcaggllgcv.............................................
A0A160FPA1_9BURK/1304-1539             gftwkgdfraledrgaisldatrhtvkintpfqfarndnqflsldpvqsthpvpqsgfelfqlgqvddivfssf------...---------------------..----.--------------..-.--.--------------------------..-----.----.--.....D.EKIGF.ISGSSS...SNENVEVLMVPAPEGGAIGIRLNFEDIDKVRPLLVSPGALSDSIFMMGVskQE..GCVLFYHISKP.SVDS...VASARRDStIER...LLEVHNSLM...TsSSM.R.MALPE..........DVaM.GDIQLLARlFDYANIVYS---------...----.........---------------..-.---------------.--.---.------------------------dpsgatgfdvvapgspiskksaiknlsr..............................
U3ALL7_9VIBR/18-287                    .......................................................................rkk---SDD...ISTVIQGELTPLIGKGIIDNN..GSCDrLPFS--ISHVQNDA..K.--.---RQEVREESAASKPG-----LAYF..TGKNV.TSAEmLP.....G.SPIGQ.YYHQTQ...FEDNLNVLEIDNGDKGTFRISFDLNNVKEGEPLLIHGGALSGCSVVFAT..KM..NKLFALHAGQHeNEKT...VWVTGEKG.AES...IAKSIALLT...S.EDP.S.NIQCA..........NN.Q.ELVSYLSSkFDQSVLVYCGDDRPLTSE...----.........---HNVKYFDYDST-..P.ENKDPRVGNALALVS.KK.QGK.INVQVLGDDMAVDKNSFETRSIS-s.........................................................
#=GC seq_cons                          .........................................................................t..............................................h..Dt..p...........t.................h..tGtth.tttt..s.....s.h.hGt.ahspp....hssshpllplsNGppGssul+lshsplpsupPlllouGsLSGCThlaAs..cp..sthYAYHsGps.ssss...pWpTup-G.spo...lhcuHhtht...s.sps.s.hp.ts..........ss.s.sLlshhpp.a-.uhlsY.Gc.ts.sst....................th..hsY.............thG.shsll..pp.tt..h.hthhs-................p.........................................................
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