Graphene blended with SnO₂ and Pd-Pt nanocages for sensitive non-enzymatic electrochemical detection of H₂O₂ released from living cells

Anal Chim Acta. 2018 Jul 19:1014:10-18. doi: 10.1016/j.aca.2018.01.067. Epub 2018 Feb 7.

Authors

Yamin Fu¹, Di Huang¹, Congming Li¹, Lina Zou¹, Baoxian Ye²

Affiliations

¹ College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China.
² College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001, PR China. Electronic address: yebx@zzu.edu.cn.

PMID: 29523247
DOI: 10.1016/j.aca.2018.01.067

Abstract

This paper described a novel, facile and nonenzymatic electrochemical biosensor to detect hydrogen peroxide (H₂O₂). The sensor was fabricated based on Pd-Pt nanocages and SnO₂/graphene nanosheets modified electrode (PdPt NCs@SGN/GCE). The electrochemical behavior of PdPt NCs@SGN/GCE exhibited excellent catalytic activity toward H₂O₂ with fast response, high selectivity, superior sensitivity, low detection limit of 0.3 μM and large linear range from 1 μM to 300 μM. Under these obvious advantages, the constructed biosensor provided to be reliable for determination of H₂O₂ secreted from human cervical cancer cells (Hela cells). Hence, the proposed biosensor is a promising candidate for detection of H₂O₂ in situ released from living cells in clinical diagnostics.

Keywords: Electrochemistry; Hela cell; Hydrogen peroxide; Pd-Pt nanocages; SnO(2).

MeSH terms

Electrochemical Techniques*
Graphite / chemistry*
HeLa Cells
Humans
Hydrogen Peroxide / analysis*
Nanoparticles / chemistry*
Palladium / chemistry
Platinum / chemistry
Tin Compounds / chemistry
Tumor Cells, Cultured

Substances

Tin Compounds
Platinum
Palladium
Graphite
Hydrogen Peroxide
stannic oxide