Photo-triggered recognition between host and guest compounds in a giant vesicle encapsulating photo-pierceable vesicles

Chem Phys Lipids. 2018 Jan:210:70-75. doi: 10.1016/j.chemphyslip.2017.11.008. Epub 2017 Nov 9.

Authors

Kentaro Suzuki¹, Kotaro Machida², Kazuo Yamaguchi², Tadashi Sugawara²

Affiliations

¹ Department of Chemistry, Faculty of Sciences, Kanagawa University, 2946 Tsuchiya, Hiratsuka, Kanagawa, 259-1293, Japan. Electronic address: suzuken@kanagawa-u.ac.jp.
² Department of Chemistry, Faculty of Sciences, Kanagawa University, 2946 Tsuchiya, Hiratsuka, Kanagawa, 259-1293, Japan.

PMID: 29129571
DOI: 10.1016/j.chemphyslip.2017.11.008

Abstract

Here, we used centrifugal precipitation to construct a giant vesicle (GV) encapsulating smaller giant vesicles (GV-in-GV) which comprises a photo-resistant outer GV and a photo-pierceable inner GV; the outer GV contained a fluorescent probe (SYBR Green I) in its inner water pool, and the inner GV contained double-stranded DNA (dsDNA) in its inner water pool. The phospholipid membrane of the inner GV was made photo-pierceable by inclusion of ca. 15mol% of a caged phospholipid in its membrane. Immediately after exposure of the GV-in-GVs to UV irradiation, strong fluorescence was detected in the inner water pool of the outer GV, indicating that dsDNA had been released from the inner GV and had complexed with the fluorescent probe. These dynamics can be recognized as a macroscopic representation of the molecular level function of a caged compound.

Keywords: Caged compound; Fluorescence probe; Giant vesicle; Photo-pierceable GV; Photo-triggered recognition; SYBR Green I; Vesosome; dsDNA.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

DNA / chemistry*
Fluorescent Dyes / chemistry*
Lipid Bilayers / chemistry*
Molecular Structure
Particle Size
Phospholipids / chemistry*
Photochemical Processes
Surface Properties
Water / chemistry*

Substances

Fluorescent Dyes
Lipid Bilayers
Phospholipids
Water
DNA