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In vitro activity of mastoparan-AF alone and in combination with clinically used antibiotics against multiple-antibiotic-resistant Escherichia coli isolates from animals

Peptides. 2012 Jul;36(1):114-20. doi: 10.1016/j.peptides.2012.03.002. Epub 2012 Apr 26.

Abstract

The in vitro activity of mastoparan-AF, an amphipathic antimicrobial peptide isolated from the hornet venom of Vespa affinis, alone and in combination with various clinically used antibiotics, was investigated against 21 Escherichia coli isolates/strains. Most E. coli isolates tested were detected containing multiple-antimicrobial resistance genes. Antimicrobial activity of mastoparan-AF was measured by MIC, MBC, time-kill kinetic assay and chequerboard titration method. Mastoparan-AF exhibited potent antimicrobial activity against most multiple-antibiotic-resistant E. coli isolates at the concentrations ranging from 4 to 16 μg/ml. Combination studies showed that mastoparan-AF acts synergistically with certain antibiotics, i.e., cephalothin or gentamicin, against some multiple-antibiotic-resistant E. coli isolates. In conclusion, mastoparan-AF alone or in combination with other antibiotics could be promising as alternatives for combating multiple-antibiotic-resistant E. coli in future clinical applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Ampicillin / pharmacology
  • Animals
  • Anti-Bacterial Agents / pharmacology*
  • Antimicrobial Cationic Peptides / pharmacology*
  • Cephalothin / pharmacology
  • Chloramphenicol / pharmacology
  • Diarrhea / microbiology
  • Diarrhea / veterinary*
  • Drug Resistance, Multiple, Bacterial / genetics*
  • Drug Synergism
  • Escherichia coli / drug effects*
  • Escherichia coli / genetics
  • Escherichia coli / isolation & purification
  • Escherichia coli Infections / microbiology
  • Escherichia coli Infections / veterinary*
  • Genes, Bacterial
  • Gentamicins / pharmacology
  • Microbial Sensitivity Tests
  • Neomycin / pharmacology
  • Sus scrofa
  • Swine
  • Swine Diseases / microbiology*
  • Tetracycline / pharmacology
  • Wasp Venoms / pharmacology*

Substances

  • Anti-Bacterial Agents
  • Antimicrobial Cationic Peptides
  • Gentamicins
  • Wasp Venoms
  • mastoparan-AF
  • Chloramphenicol
  • Ampicillin
  • Tetracycline
  • Neomycin
  • Cephalothin