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Pax-3 and Pax-7 label muscle progenitor cells during myotomal myogenesis in Coregonus lavaretus (Teleostei: Coregonidae)

Anat Histol Embryol. 2009 Nov;38(6):411-8. doi: 10.1111/j.1439-0264.2009.00961.x. Epub 2009 Sep 28.

Abstract

In Coregonus lavaretus, prior the mesoderm segmentation, in cells adjacent to the notochord called adaxial cells MyoD and slow myosin heavy chain (MyHC-slow) proteins were observed. After somite formation, adaxial cells migrate towards the lateral part of the myotome and form a layer of red muscles. Deeper cells differentiate into white muscle fibres. In situ hybridization using Pax-3 molecular probe revealed, that after somitogenesis, Pax-3 is expressed in a layer of cells superficial to the myotome resembling the "external cells" (found in many teleosts species) or dermomyotome described in Amniota. During later developmental stages Pax-3 gene is expressed in cells in intermyotomal space and then in myoblasts between myotubes. In these cells Pax-7 protein was also observed. Pax-3/7 positive cells which have migrated into the myotomes differentiate into satellite cells/secondary myoblasts and participate in hypertrophic and hyperplastic growth of muscles.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Mesoderm / embryology
  • Muscle Development / physiology*
  • Muscle Fibers, Fast-Twitch / physiology
  • Muscle Fibers, Skeletal
  • Muscle, Skeletal / embryology
  • Muscle, Skeletal / growth & development
  • MyoD Protein / metabolism
  • Myoblasts / metabolism*
  • Myosin Heavy Chains / metabolism
  • Notochord / embryology
  • PAX3 Transcription Factor
  • PAX7 Transcription Factor / genetics*
  • PAX7 Transcription Factor / metabolism*
  • Paired Box Transcription Factors / genetics*
  • Paired Box Transcription Factors / metabolism*
  • Salmonidae / embryology
  • Salmonidae / metabolism*
  • Satellite Cells, Skeletal Muscle / physiology
  • Somites / embryology
  • Zebrafish Proteins / genetics*
  • Zebrafish Proteins / metabolism*

Substances

  • MyoD Protein
  • PAX3 Transcription Factor
  • PAX7 Transcription Factor
  • Paired Box Transcription Factors
  • Zebrafish Proteins
  • pax3a protein, zebrafish
  • Myosin Heavy Chains