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Zinc oxide nanoparticles-chitosan composite film for cholesterol biosensor

Anal Chim Acta. 2008 Jun 2;616(2):207-13. doi: 10.1016/j.aca.2008.04.010. Epub 2008 Apr 11.

Abstract

Zinc oxide nanoparticles (NanoZnO) uniformly dispersed in chitosan (CHIT) have been used to fabricate a hybrid nanocomposite film onto indium-tin-oxide (ITO) glass plate. Cholesterol oxidase (ChOx) has been immobilized onto this NanoZnO-CHIT composite film using physiosorption technique. Both NanoZnO-CHIT/ITO electrode and ChOx/NanoZnO-CHIT/ITO bioelectrode have been characterized using Fourier transform-infrared (FTIR), X-ray diffraction (XRD), cyclic voltammetry (CV), scanning electron microscopy (SEM) and electrochemical impedance spectroscopy (EIS) techniques, respectively. The ChOx/NanoZnO-CHIT/ITO bioelectrode exhibits linearity from 5 to 300 mg dl(-1) of cholesterol with detection limit as 5 mg dl(-1), sensitivity as 1.41x10(-4) A mg dl(-1) and the value of Michaelis-Menten constant (K(m)) as 8.63 mg dl(-1). This cholesterol biosensor can be used to estimate cholesterol in serum samples.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biosensing Techniques*
  • Chitosan / chemistry*
  • Cholesterol / analysis*
  • Cholesterol Oxidase / chemistry
  • Electrochemistry
  • Electrodes
  • Enzymes, Immobilized / chemistry
  • Humans
  • Indium / chemistry
  • Membranes, Artificial*
  • Microscopy, Electron, Scanning / methods
  • Nanoparticles / chemistry*
  • Oxides / chemistry
  • Particle Size
  • Photometry
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectroscopy, Fourier Transform Infrared / methods
  • Tin / chemistry
  • X-Ray Diffraction
  • Zinc Oxide / chemistry*

Substances

  • Enzymes, Immobilized
  • Membranes, Artificial
  • Oxides
  • Indium
  • Tin
  • Chitosan
  • Cholesterol
  • Cholesterol Oxidase
  • Zinc Oxide