Abstract
A new semaphorin inhibitor xanthofulvin was isolated from the cultured broth of a fungus Penicillium sp. SPF-3059 along with a known compound vinaxanthone by solvent extraction and bioassay-guided fractionation. The tautomeric structure of xanthofulvin was determined by spectroscopic analyses. The two compounds exhibited significant semaphorin inhibitory activity with IC50 values of 0.09 and 0.1 microg/ml, respectively, in semaphorin3A-induced growth cone collapse assay using cultured chick dorsal root ganglia neurons.
MeSH terms
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Animals
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Chick Embryo
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Chromones*
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Enzyme Inhibitors / chemistry*
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Enzyme Inhibitors / isolation & purification
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Enzyme Inhibitors / pharmacology*
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Fermentation
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Ganglia, Spinal / drug effects
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Ganglia, Spinal / enzymology
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Growth Cones / metabolism
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Microscopy, Electron, Scanning
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Molecular Structure
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Nuclear Magnetic Resonance, Biomolecular
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Penicillium / chemistry
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Penicillium / metabolism*
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Semaphorin-3A / antagonists & inhibitors*
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Semaphorin-3A / metabolism
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Spectrometry, Mass, Fast Atom Bombardment
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Spectrophotometry, Infrared
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Spectrophotometry, Ultraviolet
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Xanthenes / chemistry*
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Xanthenes / isolation & purification
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Xanthenes / pharmacology
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Xanthones*
Substances
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Chromones
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Enzyme Inhibitors
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Semaphorin-3A
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Xanthenes
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Xanthones
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xanthofulvin