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Evaluation of susceptibility of human herpesvirus 8 to antiviral drugs by quantitative real-time PCR

J Clin Microbiol. 2003 Aug;41(8):3897-900. doi: 10.1128/JCM.41.8.3897-3900.2003.

Abstract

A new in vitro system based on real-time PCR was developed for evaluation of human herpesvirus 8 susceptibility to antiviral agents. Cidofovir had the greatest inhibitory activity against HHV-8 (50% inhibitory concentration [IC(50)], 0.43 microM) followed by ganciclovir (2.61 microM), adefovir (18.00 microM), acyclovir (31.00 microM), and foscarnet (34.15 microM). The potential therapeutic efficacy for HHV-8 (i.e., peak serum drug level/IC(50)) is highest for cidofovir (167) and foscarnet (22).

MeSH terms

  • Antiviral Agents / blood
  • Antiviral Agents / pharmacology*
  • Cidofovir
  • Cytosine / analogs & derivatives*
  • Cytosine / blood
  • Cytosine / pharmacology*
  • Ganciclovir / blood
  • Ganciclovir / pharmacology
  • Herpesvirus 8, Human / drug effects*
  • Herpesvirus 8, Human / genetics
  • Humans
  • Microbial Sensitivity Tests
  • Organophosphonates*
  • Organophosphorus Compounds / blood
  • Organophosphorus Compounds / pharmacology*
  • Polymerase Chain Reaction / methods
  • Regression Analysis

Substances

  • Antiviral Agents
  • Organophosphonates
  • Organophosphorus Compounds
  • Cytosine
  • Cidofovir
  • Ganciclovir