Abstract
Recent studies in humans have highlighted the importance of a distinct cellular entity, the plasmacytoid dendritic cell (PDC). To identify genes for which expression is restricted to human PDCs, a cDNA subtraction technique was applied using cDNA from activated monocyte-derived DCs (MDDCs) as competitor. In the 650 sequences analyzed, 25% were for B-cell transcripts. We also found lymphoid-related genes, immunoglobulinlike transcript 7 (ILT7), granzyme B (GrB), Spi-B, and the receptor tyrosine kinase Eph-B1. Granzyme B was up-regulated on activation, and protein was detected only in PDCs. Eph-B1 protein was expressed in the cytoplasm and the nuclei of PDCs and MDDCs, respectively. Interestingly, several novel molecules have been identified that were predicted to encode for a type 2 transmembrane protein (BRI(3)), a putative cytokine (C-15, a cysteine-rich-secreted protein), and a type 1 leucine-rich repeat protein (MAPA). The identification of genes expressed in PDCs provides new insights into their function and origin.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Amino Acid Sequence
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Animals
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Chromosome Mapping
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Cytokines / biosynthesis
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Cytokines / genetics
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DNA, Complementary / genetics
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / genetics
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Dendritic Cells / classification
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Dendritic Cells / metabolism*
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Ephrin-B1 / biosynthesis*
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Ephrin-B1 / genetics
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Expressed Sequence Tags
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Gene Expression Profiling
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Gene Library
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Granzymes
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Humans
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Membrane Proteins / biosynthesis
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Membrane Proteins / genetics
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Mice
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Molecular Sequence Data
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Monocytes / cytology
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Multigene Family
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Organ Specificity
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Protein Biosynthesis
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Proteins / genetics
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RNA, Messenger / biosynthesis*
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RNA, Messenger / genetics
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Repetitive Sequences, Amino Acid
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Reverse Transcriptase Polymerase Chain Reaction
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Sequence Alignment
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Sequence Homology, Amino Acid
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Serine Endopeptidases / biosynthesis*
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Serine Endopeptidases / genetics
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Species Specificity
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Subtraction Technique
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Transcription Factors / biosynthesis
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Transcription Factors / genetics
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Transcription, Genetic
Substances
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Cytokines
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DNA, Complementary
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DNA-Binding Proteins
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Ephrin-B1
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ITM2C protein, human
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LRRC25 protein, human
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Membrane Proteins
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PLAC8 protein, human
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Proteins
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RNA, Messenger
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Transcription Factors
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SPIB protein, human
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GZMB protein, human
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Granzymes
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Gzmb protein, mouse
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Serine Endopeptidases
Associated data
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GENBANK/AJ422147
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GENBANK/AJ422148
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GENBANK/AJ422149
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GENBANK/AJ422150
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GENBANK/AJ422151