Laying hens, Gallus gallus domesticusshow individual variation in pre-laying behaviour including their ultimate choice of nest site. In housing systems with nestboxes, the majority of hens make a small number of long visits to nestboxes and lay their eggs therein, but some hens make many short visits and occasionally lay outside the nestbox. We investigated the motivational basis of this individual variation using six consistent hens which always laid in nestboxes and six inconsistent hens which sometimes laid outside nestboxes. Each hen was housed in a pen (containing either no nestbox, a semi-enclosed nestbox or an enclosed nestbox) with access to a ring-shaped tunnel which increased the opportunity to perform locomotor activity. Access to the tunnel could be restricted by narrowing the doorway to 140, 125, 110 or 95 mm (compared with a mean hen width of 114 mm). In trials with no nestbox, there was no difference in the pre-laying behaviour of consistent and inconsistent hens. Narrowing the doorway reduced the number of visits to the tunnel, but all hens persisted in visiting the tunnel and doorwidth had no effect on time spent therein. With both designs of nestbox, however, inconsistent hens visited the tunnel more often than consistent hens prior to oviposition, and continued to pass the narrowest doors to enter the tunnel, whilst consistent hens would not pass doors of 110 or 95 mm. After oviposition, there was no difference in the two groups' behaviour in any treatment and no hens would pass doors of either 110 or 95 mm to visit the tunnel. Individual variation in nest-site choice, therefore, appeared to result from different perception of nestboxes rather than lower nesting motivation. Inconsistent hens worked as hard as consistent hens to perform pre-laying locomotion, but appeared to be less responsive to the cues provided by nestboxes than consistent hens, because they persisted with pre-laying locomotion when provided with either design of nestbox.Copyright 1997 The Association for the Study of Animal Behaviour1997The Association for the Study of Animal Behaviour