Abstract
We have examined whether the stress-induced transcriptional activation of YDR1/PDR5/STS1 is mediated by yAP-1 and yAP-2. Of the stresses examined, heat shock-induced, rapid and transient PDR5 expression became very low in a yap1 yap2 double-gene disruptant, indicating that the yAP proteins mediate the response. Similar results were obtained with SNQ2, a close homologue of PDR5. A set of 5'-truncation derivatives of the PDR5 gene identified the region from -484 to -434 as being sufficient for the response. A sequence similar to the yAP-1 recognition element recently identified in the stress-responsive yeast genes was found in this region and in the 5'-flanking sequences of SNQ2.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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ATP-Binding Cassette Transporters / genetics*
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Adaptor Protein Complex alpha Subunits
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Adaptor Proteins, Vesicular Transport
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Base Sequence
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DNA-Binding Proteins / metabolism
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DNA-Binding Proteins / physiology*
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Drug Resistance, Multiple / genetics*
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Fungal Proteins / genetics
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Gene Expression Regulation, Fungal*
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Hot Temperature
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Membrane Proteins / genetics
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Molecular Sequence Data
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Saccharomyces cerevisiae / genetics
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Saccharomyces cerevisiae / physiology*
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Saccharomyces cerevisiae Proteins*
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Transcription Factors / metabolism
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Transcription Factors / physiology*
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Transcription, Genetic
Substances
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ATP-Binding Cassette Transporters
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Adaptor Protein Complex alpha Subunits
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Adaptor Proteins, Vesicular Transport
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DNA-Binding Proteins
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Fungal Proteins
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Membrane Proteins
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PDR5 protein, S cerevisiae
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STS1 protein, S cerevisiae
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Saccharomyces cerevisiae Proteins
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Transcription Factors
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YAP1 protein, S cerevisiae