|
| Status |
Public on Sep 12, 2013 |
| Title |
Transcriptional profiles by deep sequencing (RNA-seq) of in vivo-generated mouse iPSCs, in vitro-generated mouse iPSCs, and mouse ESCs |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have generated “reprogrammable” transgenic mice that ubiquitously express the four Yamanaka factors in an inducible manner. Transitory induction of the transgene results in multiple teratomas emerging from a variety of organs, thus indicating that full reprogramming into iPSCs can occur in vivo. By performing bone marrow transplant experiments, we demonstrate that both hematopoietic cells, as well as non-hematopoietic cells can be reprogrammed in vivo. Remarkably, reprogrammable mice also present circulating iPSCs in the bloodstream (in vivo-iPSCs) with all the expected properties of bona fide iPSCs. Moreover, in contrast to in vitro-iPSCs or embryonic stem cells (ESCs), in vivo-iPSCs have an increased capacity to undergo trophectoderm lineage differentiation, which suggests that in vivo-iPSCs are more plastic or primitive than in vitro-generated iPSCs or ESCs.
|
| |
|
| Overall design |
6 clones of in vivo-generated iPSCs, 5 indendent clones of in vitro-generated iPSCs, and 3 clones of established ESCs
|
| |
|
| Contributor(s) |
Abad M, Graña O, Serrano M |
| Citation(s) |
24025773 |
| |
| Submission date |
Jun 27, 2013 |
| Last update date |
May 15, 2019 |
| Contact name |
Manuel Serrano |
| E-mail(s) |
manuel.serrano@irbbarcelona.org
|
| Organization name |
IRB
|
| Department |
Molecular Medicine
|
| Lab |
Cellular Plasticity and Disease
|
| Street address |
Parc Científic de Barcelona, C/ Baldiri Reixac 10
|
| City |
Barcelona |
| State/province |
Barcelona |
| ZIP/Postal code |
08028 |
| Country |
Spain |
| |
|
| Platforms (1) |
| GPL11002 |
Illumina Genome Analyzer IIx (Mus musculus) |
|
| Samples (14)
|
|
| Relations |
| BioProject |
PRJNA209846 |
| SRA |
SRP026364 |